Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,677,885	A→G	Y102C (TAT→TGT)	b1605 →	putative arginine/ornithine antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,677,885	0	A	G	99.6%	763.9 / ‑5.4	236	Y102C (TAT→TGT)	b1605	putative arginine/ornithine antiporter
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (123/112); total (123/113)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 247 total aligned reads displayed.

TTACCTATGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATAG  >  NC_000913/1677795‑1677974

ttACCTATGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCCACGTCTCCTGTc                                                                                         >  2:3729125/1‑93 (MQ=255)

ttACCTATGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTc                                                                                         >  1:502852/1‑93 (MQ=255)

ttACCTATGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTc                                                                                         <  2:2545374/93‑1 (MQ=255)

 tACCTATGTCAGCGAAGGGCTTGGATAGCTAATAGGATTTTGTTCGGCATGTGCATACTGGCTGTGCGCAGCAATAGAGAACGTCTCCTGTCt                                                                                        >  2:4315141/1‑93 (MQ=255)

 tACCTATGCCCGCGTAGGGGTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAAAGTCGCCTGTCt                                                                                        >  2:2380883/1‑93 (MQ=255)

   ccTATGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTgg                                                                                      >  2:2164774/1‑93 (MQ=255)

    cTATGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGt                                                                                     >  1:6371339/1‑93 (MQ=255)

     tATGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATAGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTg                                                                                    >  2:8287378/1‑93 (MQ=255)

       tGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGAt                                                                                  <  2:810159/93‑1 (MQ=255)

       tGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGAt                                                                                  <  2:1256812/93‑1 (MQ=255)

       tGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGAt                                                                                  <  1:6530160/93‑1 (MQ=255)

          ccGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGCCTGGGGATCGt                                                                               >  2:7041581/1‑93 (MQ=255)

           cgcgAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGtt                                                                              >  2:69179/1‑93 (MQ=255)

           cgcgAAGGGTCTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCgct                                                                              >  2:8343923/1‑91 (MQ=255)

             cgAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGGGATCGtttt                                                                            >  1:902099/1‑93 (MQ=255)

             cgAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGACAACGTCTCCTGTCTGGTGATCGtttt                                                                            >  2:4041271/1‑93 (MQ=255)

               aaGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGtttttt                                                                          >  2:9416967/1‑93 (MQ=255)

                aGGGTTTGGCGACCTAATCGGCTTTTGCTCCGCATAGGGATACTGGCTGTGCGCAGGCATCGCCAACGGCTCCTGTCTGGTGAGCGttttttg                                                                         >  2:7542763/1‑92 (MQ=255)

                 gggTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTcc                                                                        >  1:7322716/1‑93 (MQ=255)

                    tttGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCcgcg                                                                     >  2:1111082/1‑93 (MQ=255)

                        gCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTaa                                                                 <  1:4049062/93‑1 (MQ=255)

                         ggAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAg                                                                <  1:1227977/92‑1 (MQ=255)

                         cGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAg                                                                >  1:9926168/1‑93 (MQ=255)

                           aGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCt                                                              >  1:1241273/1‑93 (MQ=255)

                            gCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCtt                                                             <  1:6074377/93‑1 (MQ=255)

                             cTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCttt                                                            <  1:7882343/93‑1 (MQ=255)

                               aaTCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCttttt                                                          >  2:6298002/1‑93 (MQ=255)

                               aaTCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCttttt                                                          <  1:1698396/93‑1 (MQ=255)

                               aaTCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCttttt                                                          >  2:328177/1‑93 (MQ=255)

                               aaTCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCttttt                                                          <  1:3553799/93‑1 (MQ=255)

                                 tCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTa                                                        >  1:883203/1‑93 (MQ=255)

                                  cGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTAc                                                       <  2:8702433/93‑1 (MQ=255)

                                  cGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTAc                                                       <  1:2097052/93‑1 (MQ=255)

                                  cGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTAc                                                       <  1:8088098/93‑1 (MQ=255)

                                     cTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGa                                                    >  2:6115361/1‑93 (MQ=255)

                                     cTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGa                                                    <  1:6800524/93‑1 (MQ=255)

                                       tttGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTTTGGTGATCGTTTTTGCCGCGTTAAGCTTTTTTACGGaca                                                  <  2:10020628/93‑1 (MQ=255)

                                       tttGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGaca                                                  >  1:5935670/1‑93 (MQ=255)

                                       tttGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGaca                                                  >  2:4612929/1‑93 (MQ=255)

                                        ttGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGacac                                                 <  1:5603281/93‑1 (MQ=255)

                                          tttCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGc                                               <  1:2799261/92‑1 (MQ=255)

                                          gTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGc                                               <  1:10000746/93‑1 (MQ=255)

                                           ttCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGcc                                              <  1:387182/93‑1 (MQ=255)

                                               gCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGaa                                          <  2:7365460/93‑1 (MQ=255)

                                                cATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAAt                                         <  1:5382499/93‑1 (MQ=255)

                                                cATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAAt                                         >  1:5996763/1‑93 (MQ=255)

                                                 aTGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAAtt                                        >  1:9508099/1‑93 (MQ=255)

                                                  tGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTg                                       >  1:4767849/1‑93 (MQ=255)

                                                  tGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTg                                       >  2:1274532/1‑93 (MQ=255)

                                                    gggATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTgcg                                     >  1:7174746/1‑93 (MQ=255)

                                                     ggATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTgcgc                                    >  1:711802/1‑93 (MQ=255)

                                                        tACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTg                                 >  1:7646274/1‑93 (MQ=255)

                                                          cTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGtt                               >  1:4346366/1‑93 (MQ=255)

                                                          cTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGtt                               <  1:6674020/93‑1 (MQ=255)

                                                          cTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGtt                               <  1:3536754/93‑1 (MQ=255)

                                                           tGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGttt                              >  2:1429671/1‑93 (MQ=255)

                                                            ggCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTg                             <  1:3353686/93‑1 (MQ=255)

                                                             gCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTgg                            >  1:1772805/1‑93 (MQ=255)

                                                              cTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGc                           <  1:3102192/93‑1 (MQ=255)

                                                               tgtgCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCg                          <  2:5826896/93‑1 (MQ=255)

                                                                gtgCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGa                         >  2:6435319/1‑93 (MQ=255)

                                                                 tgCGCAGTCATCGCCCACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTAAGGACCCGCCGGAATTGCGCCCGTTTGGCGac                        >  1:2407996/1‑92 (MQ=255)

                                                                 tacacAGTCATCGACAACGTCTCCTGACAGGTGATAGTTTTTTCCGCGATAAGAATTATTACGGCCACGCCGGAATTGCGCATGTTTGGCGAt                        <  2:9243513/89‑1 (MQ=255)

                                                                   cgcAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGTAATTGCGCCTGTTTGGCGATgg                      >  2:8660797/1‑93 (MQ=255)

                                                                   cgcAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATgg                      >  1:3191165/1‑93 (MQ=255)

                                                                   cgcAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATgg                      <  1:3468515/93‑1 (MQ=255)

                                                                   cgcAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATgg                      <  1:8076568/93‑1 (MQ=255)

                                                                    gcAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGt                     >  1:2857578/1‑93 (MQ=255)

                                                                    gcAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGACGGAATTGCGCCTGTTTGGCGATGGt                     <  2:7425654/93‑1 (MQ=255)

                                                                     cAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTa                    >  1:9249771/1‑93 (MQ=255)

                                                                       gTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAAt                  >  1:2216568/1‑93 (MQ=255)

                                                                        tCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATa                 >  2:348944/1‑93 (MQ=255)

                                                                        tCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATa                 >  2:2018272/1‑93 (MQ=255)

                                                                         cATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATAc                <  2:6571141/93‑1 (MQ=255)

                                                                          aTCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATAcc               <  1:4185816/93‑1 (MQ=255)

                                                                            cGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTAGGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTg             <  1:7350568/93‑1 (MQ=255)

                                                                            cGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTg             <  2:6515492/93‑1 (MQ=255)

                                                                            cGCCAACGGCTCCGGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTg             <  2:2912172/93‑1 (MQ=255)

                                                                             gcgaaCGTCTCCTGTCTGGTGATCGTGTTTTCCGCGTTAAGCTTTTTTACGGTCACGCGGTAATTGCGCCGGTTCGGCGATGGTAATACCTgg            <  2:8867410/90‑1 (MQ=255)

                                                                             gCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTgg            >  2:4987123/1‑93 (MQ=255)

                                                                                aaCGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCaa         >  1:994380/1‑93 (MQ=255)

                                                                                 aCGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAAt        >  2:7967556/1‑93 (MQ=255)

                                                                                 aCGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAAt        <  2:9777582/93‑1 (MQ=255)

                                                                                  cGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATc       >  2:7836507/1‑93 (MQ=255)

                                                                                  cGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATc       <  2:5874353/93‑1 (MQ=255)

                                                                                   gTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCg      >  1:3748040/1‑93 (MQ=255)

                                                                                    tctcCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGa     <  2:3833153/93‑1 (MQ=255)

                                                                                    tctcCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGa     >  2:2701829/1‑93 (MQ=255)

                                                                                    tctcCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGa     >  2:1008608/1‑93 (MQ=255)

                                                                                    tctcCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGa     <  1:5171651/93‑1 (MQ=255)

                                                                                     ctcCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGAt    >  1:6172839/1‑93 (MQ=255)

                                                                                      tcCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATa   >  2:3914713/1‑93 (MQ=255)

                                                                                      tcCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATa   >  2:2633450/1‑93 (MQ=255)

                                                                                      tcCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATa   <  1:2332703/93‑1 (MQ=255)

                                                                                      tcCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATa   <  1:8360305/93‑1 (MQ=255)

                                                                                       ccTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATAg  <  2:5245698/93‑1 (MQ=255)

                                                                                       ccTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATAg  <  2:4104251/93‑1 (MQ=255)

                                                                                       ccTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATAg  <  1:8165954/93‑1 (MQ=255)

                                                                                       ccTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATAg  <  2:1704915/93‑1 (MQ=255)

                                                                                       ccTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATAACTAGCAATAGAtcg  >  2:1583747/1‑91 (MQ=255)

TTACCTATGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATAG  >  NC_000913/1677795‑1677974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TCTTTACCTATGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATAGTCGG  >  NC_000913/1677792‑1677978

TCTTTACCTATGCCCGCGACGGATTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGATGTGCGCCGTCATCGCCAACGTCTCCTG                                                                                               <  SRR4302540.9694294/94‑1 (MQ=60)

TCTTTACCTATGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTG                                                                                               <  SRR4302540.2547475/94‑1 (MQ=60)

TCTTTACCTATGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTG                                                                                               <  SRR4302540.2547474/94‑1 (MQ=60)

 CTTTACCTATGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGT                                                                                              <  SRR4302540.4721888/94‑1 (MQ=60)

 CTTTACCTATGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGT                                                                                              <  SRR4302540.7278461/94‑1 (MQ=60)

  TTTACCTATGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTC                                                                                             >  SRR4302540.6972162/1‑94 (MQ=60)

  TTTACCTATGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTC                                                                                             >  SRR4302540.508243/1‑94 (MQ=60)

   TTACCTATGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCT                                                                                            >  SRR4302540.7353775/1‑94 (MQ=60)

    TACCTATGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTG                                                                                           >  SRR4302540.2867250/1‑94 (MQ=60)

    TACCTATGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTG                                                                                           <  SRR4302540.9326112/94‑1 (MQ=60)

    TACCTATGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTG                                                                                           >  SRR4302540.3529065/1‑94 (MQ=60)

    TACCTATGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTG                                                                                           >  SRR4302540.184573/1‑94 (MQ=60)

      CCTATGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGT                                                                                         >  SRR4302540.6442282/1‑94 (MQ=60)

      CCTATGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGT                                                                                         >  SRR4302540.824409/1‑94 (MQ=60)

       CTATGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATGCTGGCTGTGCGCGGTCATCGCCAACGTCTCCTGTCTGGTG                                                                                        <  SRR4302540.2707215/94‑1 (MQ=60)

         ATGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGAT                                                                                      >  SRR4302540.7012176/1‑94 (MQ=60)

         ATGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGAT                                                                                      >  SRR4302540.2920191/1‑94 (MQ=60)

          TGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATC                                                                                     <  SRR4302540.6602662/94‑1 (MQ=60)

          TGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATC                                                                                     <  SRR4302540.8245306/94‑1 (MQ=60)

            CCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGT                                                                                   <  SRR4302540.5688638/94‑1 (MQ=60)

             CCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTT                                                                                  <  SRR4302540.10010660/94‑1 (MQ=60)

               GCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGGGATCGTTTT                                                                                >  SRR4302540.911680/1‑94 (MQ=60)

                  AAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTC                                                                             <  SRR4302540.6252609/94‑1 (MQ=60)

                  AAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTC                                                                             <  SRR4302540.3444364/94‑1 (MQ=60)

                   AGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCC                                                                            <  SRR4302540.6487395/94‑1 (MQ=60)

                   AGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCC                                                                            >  SRR4302540.7404501/1‑94 (MQ=60)

                    GGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCG                                                                           >  SRR4302540.7370683/1‑94 (MQ=60)

                     GGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGC                                                                          >  SRR4302540.8178280/1‑94 (MQ=60)

                       TTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGT                                                                        <  SRR4302540.5108184/94‑1 (MQ=60)

                         TGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTA                                                                      >  SRR4302540.652639/1‑94 (MQ=60)

                          GGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAA                                                                     <  SRR4302540.1028108/94‑1 (MQ=60)

                           GCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCCACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAG                                                                    >  SRR4302540.10042053/1‑94 (MQ=60)

                           GCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAG                                                                    >  SRR4302540.10042052/1‑94 (MQ=60)

                           GCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAG                                                                    <  SRR4302540.4092718/94‑1 (MQ=60)

                            GGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGC                                                                   <  SRR4302540.1240525/94‑1 (MQ=60)

                             GAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCT                                                                  >  SRR4302540.1253941/1‑94 (MQ=60)

                              AGCTAATCGGCTTGTGTTCCGCATGGGGATGCTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTT                                                                 <  SRR4302540.9343476/94‑1 (MQ=60)

                               GCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTT                                                                >  SRR4302540.6827791/1‑94 (MQ=60)

                               GCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTT                                                                <  SRR4302540.6141924/94‑1 (MQ=60)

                               GCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTT                                                                <  SRR4302540.6141925/94‑1 (MQ=60)

                                CTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTT                                                               <  SRR4302540.7974381/94‑1 (MQ=60)

                                CTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTT                                                               <  SRR4302540.7870927/94‑1 (MQ=60)

                                 TAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTT                                                              >  SRR4302540.5142448/1‑94 (MQ=60)

                                 TAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTT                                                              >  SRR4302540.419922/1‑94 (MQ=60)

                                  AATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTT                                                             <  SRR4302540.8695704/94‑1 (MQ=60)

                                  AATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTT                                                             <  SRR4302540.3592627/94‑1 (MQ=60)

                                  AATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTT                                                             <  SRR4302540.1716795/94‑1 (MQ=60)

                                  AATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTT                                                             <  SRR4302540.5537745/94‑1 (MQ=60)

                                  AATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTT                                                             <  SRR4302540.4323943/94‑1 (MQ=60)

                                  AATCGGCTTTTGTGCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTT                                                             <  SRR4302540.7925882/94‑1 (MQ=60)

                                   ATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTA                                                            >  SRR4302540.892573/1‑94 (MQ=60)

                                   ATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTA                                                            >  SRR4302540.3895432/1‑94 (MQ=60)

                                   ATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTCTTGTCCGCGTTAAGCTTTTTTA                                                            >  SRR4302540.9035484/1‑94 (MQ=60)

                                     CGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACG                                                          <  SRR4302540.2119566/94‑1 (MQ=60)

                                     CGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACG                                                          <  SRR4302540.8181984/94‑1 (MQ=60)

                                      GGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGG                                                         >  SRR4302540.7250414/1‑94 (MQ=60)

                                       GCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGA                                                        >  SRR4302540.6221075/1‑94 (MQ=60)

                                        CTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGAC                                                       <  SRR4302540.6875769/94‑1 (MQ=60)

                                         TTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACA                                                      >  SRR4302540.6001283/1‑94 (MQ=60)

                                          TTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACAC                                                     <  SRR4302540.2080385/94‑1 (MQ=60)

                                           TTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACG                                                    <  SRR4302540.5664937/94‑1 (MQ=60)

                                            TGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGC                                                   <  SRR4302540.8035998/94‑1 (MQ=60)

                                             TTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCC                                                  <  SRR4302540.2829495/94‑1 (MQ=60)

                                             GTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCC                                                  <  SRR4302540.10117254/94‑1 (MQ=60)

                                              TTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCG                                                 <  SRR4302540.6701403/94‑1 (MQ=60)

                                              TTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCG                                                 <  SRR4302540.391187/94‑1 (MQ=60)

                                                CCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGA                                               >  SRR4302540.8595592/1‑94 (MQ=60)

                                                CCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGA                                               >  SRR4302540.8389751/1‑94 (MQ=60)

                                                 CGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAA                                              >  SRR4302540.1445107/1‑94 (MQ=60)

                                                  GCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAAT                                             >  SRR4302540.6063215/1‑94 (MQ=60)

                                                  GCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAAT                                             <  SRR4302540.2616250/94‑1 (MQ=60)

                                                   CATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATT                                            >  SRR4302540.9620736/1‑94 (MQ=60)

                                                   CATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATT                                            <  SRR4302540.5441704/94‑1 (MQ=60)

                                                   CATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATT                                            <  SRR4302540.3154561/94‑1 (MQ=60)

                                                    ATGGGGATACTGGCTGTGCTCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTG                                           <  SRR4302540.9895808/94‑1 (MQ=60)

                                                    ATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTG                                           >  SRR4302540.7499425/1‑94 (MQ=60)

                                                    ATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTG                                           >  SRR4302540.4818768/1‑94 (MQ=60)

                                                     TGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGC                                          <  SRR4302540.751735/94‑1 (MQ=60)

                                                      GGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCG                                         >  SRR4302540.7254717/1‑94 (MQ=60)

                                                       GGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGGATTGCGC                                        >  SRR4302540.6060192/1‑94 (MQ=60)

                                                       GGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGC                                        <  SRR4302540.2364416/94‑1 (MQ=60)

                                                       GGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGC                                        >  SRR4302540.719445/1‑94 (MQ=60)

                                                       GGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGC                                        >  SRR4302540.8143354/1‑94 (MQ=60)

                                                          ATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTG                                     >  SRR4302540.7732340/1‑94 (MQ=60)

                                                            ACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTT                                   >  SRR4302540.4393661/1‑94 (MQ=60)

                                                             CTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTT                                  >  SRR4302540.5493111/1‑94 (MQ=60)

                                                             CTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTT                                  <  SRR4302540.6747755/94‑1 (MQ=60)

                                                             CTGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTT                                  <  SRR4302540.3575370/94‑1 (MQ=60)

                                                              TGGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTG                                 <  SRR4302540.7432380/94‑1 (MQ=60)

                                                               GGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGG                                <  SRR4302540.3390442/94‑1 (MQ=60)

                                                               GGCTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGG                                >  SRR4302540.1792043/1‑94 (MQ=60)

                                                                 CTGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCG                              <  SRR4302540.3136166/94‑1 (MQ=60)

                                                                 ATGTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCG                              <  SRR4302540.328922/94‑1 (MQ=60)

                                                                   GTGCGCAGTCATCGCCCACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTAAGGACCCGCCGGAATTGCGCCCGTTTGGCGAC                            >  SRR4302540.2433960/1‑94 (MQ=60)

                                                                   GTGCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGAT                            <  SRR4302540.2234730/94‑1 (MQ=60)

                                                                     GCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGG                          <  SRR4302540.2266773/94‑1 (MQ=60)

                                                                     GCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGG                          >  SRR4302540.3225828/1‑94 (MQ=60)

                                                                     GCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGG                          <  SRR4302540.6017532/94‑1 (MQ=60)

                                                                     GCGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGG                          <  SRR4302540.1421747/94‑1 (MQ=60)

                                                                      CGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGT                         >  SRR4302540.2888537/1‑94 (MQ=60)

                                                                      CGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGT                         <  SRR4302540.3506278/94‑1 (MQ=60)

                                                                      CGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGT                         <  SRR4302540.3506279/94‑1 (MQ=60)

                                                                      CGCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGT                         <  SRR4302540.8170300/94‑1 (MQ=60)

                                                                       GCAGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTA                        >  SRR4302540.9360505/1‑94 (MQ=60)

                                                                         AGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAAT                      >  SRR4302540.2240486/1‑94 (MQ=60)

                                                                         AGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAAT                      >  SRR4302540.935324/1‑94 (MQ=60)

                                                                         AGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAAT                      >  SRR4302540.3251881/1‑94 (MQ=60)

                                                                         AGTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAAT                      >  SRR4302540.5080781/1‑94 (MQ=60)

                                                                          GTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATA                     >  SRR4302540.2925268/1‑94 (MQ=60)

                                                                          GTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATA                     >  SRR4302540.6115970/1‑94 (MQ=60)

                                                                          GTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATA                     <  SRR4302540.10088392/94‑1 (MQ=60)

                                                                          GTCATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATA                     >  SRR4302540.5424146/1‑94 (MQ=60)

                                                                            CATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACC                   >  SRR4302540.4764032/1‑94 (MQ=60)

                                                                             ATCGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCT                  <  SRR4302540.4231309/94‑1 (MQ=60)

                                                                               CGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTAGGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGG                <  SRR4302540.7432566/94‑1 (MQ=60)

                                                                               CGCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGG                >  SRR4302540.1252596/1‑94 (MQ=60)

                                                                                GCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGC               >  SRR4302540.4179856/1‑94 (MQ=60)

                                                                                GCCAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGC               >  SRR4302540.1717164/1‑94 (MQ=60)

                                                                                  CAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAA             >  SRR4302540.1004508/1‑94 (MQ=60)

                                                                                  CAACGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAA             >  SRR4302540.3673074/1‑94 (MQ=60)

                                                                                     CGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCG          <  SRR4302540.4663052/94‑1 (MQ=60)

                                                                                     CGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCG          >  SRR4302540.3788937/1‑94 (MQ=60)

                                                                                     CGTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCG          >  SRR4302540.3788939/1‑94 (MQ=60)

                                                                                      GTCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGA         >  SRR4302540.2288445/1‑94 (MQ=60)

                                                                                       TCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGAT        <  SRR4302540.5227933/94‑1 (MQ=60)

                                                                                       TCTCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGAT        >  SRR4302540.6241616/1‑94 (MQ=60)

                                                                                         TCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATAG      <  SRR4302540.3577281/94‑1 (MQ=60)

                                                                                         TCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATAG      <  SRR4302540.8457319/94‑1 (MQ=60)

                                                                                         TCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATAG      <  SRR4302540.2357839/94‑1 (MQ=60)

                                                                                         TCCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATAG      <  SRR4302540.3964591/94‑1 (MQ=60)

                                                                                          CCTGTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATAGT     <  SRR4302540.8260780/94‑1 (MQ=60)

                                                                                             GTCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATAGTCGG  <  SRR4302540.2675349/94‑1 (MQ=60)

TCTTTACCTATGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATAGTCGG  >  NC_000913/1677792‑1677978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`