Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,680,393 T→C W74R (TGG→CGG)  rstA → response transcriptional regulatory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,680,3930TC100.0% 812.8 / NA 250W74R (TGG→CGG) rstAresponse transcriptional regulatory protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (105/145);  total (105/145)

Only 100 of 260 total aligned reads displayed.

ATTTTGCGAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTC  >  NC_000913/1680303‑1680482
                                                                                          |                                                                                         
aTTTTGCGAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCgg                                                                                         >  2:819005/1‑93 (MQ=255)
aTTTTGCGAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCgg                                                                                         <  2:6248016/93‑1 (MQ=255)
  tttGCGAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTc                                                                                       <  1:3890656/93‑1 (MQ=255)
    tGCGAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTg                                                                                     <  2:9451110/93‑1 (MQ=255)
    tGCGAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTg                                                                                     <  1:3273775/93‑1 (MQ=255)
       gagaAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGAc                                                                                  >  1:1621645/1‑93 (MQ=255)
       gagaAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGAc                                                                                  >  1:661018/1‑93 (MQ=255)
       gagaAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGAc                                                                                  <  2:8444625/93‑1 (MQ=255)
       gagaAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGAc                                                                                  <  1:8029059/93‑1 (MQ=255)
         gaAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCg                                                                                >  1:8579095/1‑93 (MQ=255)
          aaaaTCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGa                                                                               <  2:4339525/93‑1 (MQ=255)
          aaaaTCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGa                                                                               >  1:3467120/1‑93 (MQ=255)
            aaTCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGAtt                                                                             <  1:5357572/93‑1 (MQ=255)
            aaTCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGAtt                                                                             >  1:6644411/1‑93 (MQ=255)
             aTCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTg                                                                            <  2:2300931/93‑1 (MQ=255)
                cGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGttc                                                                         >  2:1789133/1‑93 (MQ=255)
                cGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGttc                                                                         <  2:8468685/93‑1 (MQ=255)
                 ggATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGttct                                                                        >  2:6193897/1‑93 (MQ=255)
                 ggATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGAGTGttct                                                                        >  2:7012819/1‑93 (MQ=255)
                    tttGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTtcttct                                                                     >  2:9783512/1‑93 (MQ=255)
                    tttGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTtcttct                                                                     <  1:3899161/93‑1 (MQ=255)
                     ttGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTa                                                                    <  2:5430231/93‑1 (MQ=255)
                      tGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTaa                                                                   <  2:7455748/93‑1 (MQ=255)
                       ggTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAAc                                                                  >  2:1428838/1‑93 (MQ=255)
                        gtgtTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAAcc                                                                 >  1:786938/1‑93 (MQ=255)
                         tgtTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACct                                                                >  1:1011783/1‑93 (MQ=255)
                         tgtTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACct                                                                <  1:4974736/93‑1 (MQ=255)
                         tgtTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACct                                                                <  1:5751479/93‑1 (MQ=255)
                          gtTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACctc                                                               >  1:3133772/1‑93 (MQ=255)
                          gtTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACctc                                                               >  2:8338264/1‑93 (MQ=255)
                           ttACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACctct                                                              >  2:8241797/1‑93 (MQ=255)
                             aCTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAATCGGTCTGGACCGATTGCTCTTCTAACctgtct                                                            >  1:2202149/1‑93 (MQ=255)
                             aCTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACctctct                                                            <  2:553704/93‑1 (MQ=255)
                                cGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGa                                                         >  2:4916595/1‑93 (MQ=255)
                                 gACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGAt                                                        <  2:3548770/93‑1 (MQ=255)
                                 gACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGAt                                                        <  1:9572740/93‑1 (MQ=255)
                                  aCATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAAGCTCTCTCGATa                                                       >  2:4544501/1‑93 (MQ=255)
                                  aCATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATa                                                       >  1:9731673/1‑93 (MQ=255)
                                  aCATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATa                                                       <  2:9664430/93‑1 (MQ=255)
                                  aCATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATa                                                       <  2:6670210/93‑1 (MQ=255)
                                  aCATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATa                                                       >  2:3206461/1‑93 (MQ=255)
                                   catcatGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAg                                                      <  1:1201539/93‑1 (MQ=255)
                                    atcatGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGc                                                     >  2:2971507/1‑93 (MQ=255)
                                    atcatGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGc                                                     >  1:40746/1‑93 (MQ=255)
                                     tcatGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCg                                                    >  1:7613447/1‑93 (MQ=255)
                                     tcatGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCg                                                    <  2:7242361/93‑1 (MQ=255)
                                     tcatGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCg                                                    >  1:4335188/1‑93 (MQ=255)
                                      catGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGa                                                   >  1:1988951/1‑93 (MQ=255)
                                        tGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGata                                                 <  1:8751730/93‑1 (MQ=255)
                                        tGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGata                                                 <  2:597394/93‑1 (MQ=255)
                                          cTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATg                                               <  2:8444459/93‑1 (MQ=255)
                                          cTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGAGATATg                                               >  2:2478136/1‑93 (MQ=255)
                                            aCCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGaa                                             >  1:2606971/1‑93 (MQ=255)
                                             ccAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAAc                                            >  2:7993989/1‑93 (MQ=255)
                                             ccAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAAc                                            <  1:4704070/93‑1 (MQ=255)
                                             ccAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAAc                                            <  1:6875986/93‑1 (MQ=255)
                                               aGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACca                                          <  1:4122030/93‑1 (MQ=255)
                                                ggCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACcac                                         <  1:9715868/93‑1 (MQ=255)
                                                ggCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACcac                                         >  1:7168117/1‑93 (MQ=255)
                                                 gCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGGTATGAACcaca                                        <  1:2323637/93‑1 (MQ=255)
                                                   aaGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATc                                      <  1:4866911/93‑1 (MQ=255)
                                                     ggACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGATATTCTAACCTCTCTCGATAGCGATATGAACCACATCCt                                    >  1:1705018/1‑93 (MQ=255)
                                                      gACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTg                                   <  1:9086576/93‑1 (MQ=255)
                                                       aCGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTgg                                  >  2:5366654/1‑93 (MQ=255)
                                                         ggCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCa                                <  2:607640/93‑1 (MQ=255)
                                                          gCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCAc                               <  2:7845117/93‑1 (MQ=255)
                                                            aTGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTg                             >  2:7877769/1‑93 (MQ=255)
                                                             tGACCATTTGTCGTGATTTACGCGCACAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTgg                            >  2:3667282/1‑93 (MQ=255)
                                                             tGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTgg                            >  2:1342105/1‑93 (MQ=255)
                                                             tGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTgg                            >  2:4055393/1‑93 (MQ=255)
                                                             tGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTgg                            >  2:9072218/1‑93 (MQ=255)
                                                             tGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTgg                            >  2:8519040/1‑93 (MQ=255)
                                                                ccATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGaaa                         <  2:7199192/93‑1 (MQ=255)
                                                                ccATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGaaa                         <  1:8685601/93‑1 (MQ=255)
                                                                ccATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGaaa                         <  2:881965/93‑1 (MQ=255)
                                                                   tttGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATgg                      <  2:4438577/93‑1 (MQ=255)
                                                                    ttGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATggg                     <  2:5749492/93‑1 (MQ=255)
                                                                      gTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTg                   <  2:2801169/93‑1 (MQ=255)
                                                                      gTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTg                   >  2:4122951/1‑93 (MQ=255)
                                                                       tCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGc                  >  2:1990212/1‑93 (MQ=255)
                                                                        cGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGcc                 <  1:4259006/93‑1 (MQ=255)
                                                                         gTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCt                <  2:9199853/93‑1 (MQ=255)
                                                                         gTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCt                <  1:5917553/93‑1 (MQ=255)
                                                                          tGATTTACGCGCAAAGCGGTCTGGACCGCTTGTTCTTCTAACCTCTCTCGATCGCGATATGAACCACATCCTCGCACTGGAACTGCGTGCCTg               >  2:7330144/1‑93 (MQ=255)
                                                                          tGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTg               <  2:2671941/93‑1 (MQ=255)
                                                                           gATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGc              <  1:6678752/93‑1 (MQ=255)
                                                                           gATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGc              >  1:2974510/1‑93 (MQ=255)
                                                                             tttACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGGCTGCGa            >  2:6654183/1‑93 (MQ=255)
                                                                                aCGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATTGGTGCCTGCGACta         <  2:1604217/93‑1 (MQ=255)
                                                                                aCGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACta         <  1:2111152/93‑1 (MQ=255)
                                                                                aCGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACta         <  1:3753059/93‑1 (MQ=255)
                                                                                 cgcgCAAAGCGGTCTGGACCGATTGTTGTTCTAACCTCTCTCGATAGGGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACtat        <  2:480563/93‑1 (MQ=255)
                                                                                 cgcgCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACtat        <  1:2442855/93‑1 (MQ=255)
                                                                                  gcgcAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACtata       <  2:5650339/93‑1 (MQ=255)
                                                                                     cAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATTGGTGCCTGCGACTATATtc    >  1:777439/1‑93 (MQ=255)
                                                                                     cAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATtc    <  2:5980479/93‑1 (MQ=255)
                                                                                      aaaGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATtct   <  1:6364077/93‑1 (MQ=255)
                                                                                      aaaGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATtct   <  1:6282465/93‑1 (MQ=255)
                                                                                       aaGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATtctc  <  1:7002558/93‑1 (MQ=255)
                                                                                       aaGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATtctc  >  1:8495622/1‑93 (MQ=255)
                                                                                          |                                                                                         
ATTTTGCGAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTC  >  NC_000913/1680303‑1680482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACCATTTTGCGAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAA  >  NC_000913/1680300‑1680485
                                                                                             |                                                                                            
ACCATTTTGCGAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGC                                                                                              <  SRR4302540.10028421/94‑1 (MQ=60)
 CCATTTTGCGAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCG                                                                                             <  SRR4302540.8896743/94‑1 (MQ=60)
 CCATTTTGCGAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCG                                                                                             >  SRR4302540.8991480/1‑94 (MQ=60)
 ACATTTTGCGAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCG                                                                                             <  SRR4302540.1785469/94‑1 (MQ=60)
   ATTTTGCGAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGT                                                                                           >  SRR4302540.6225450/1‑94 (MQ=60)
    TTTTGCGAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTC                                                                                          >  SRR4302540.3056964/1‑94 (MQ=60)
     TTTGCGAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCT                                                                                         <  SRR4302540.3932899/94‑1 (MQ=60)
       TGCGAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGG                                                                                       <  SRR4302540.3309512/94‑1 (MQ=60)
         CGAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGAC                                                                                     >  SRR4302540.668094/1‑94 (MQ=60)
         CGAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGAC                                                                                     >  SRR4302540.1639422/1‑94 (MQ=60)
          GAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACC                                                                                    <  SRR4302540.8122418/94‑1 (MQ=60)
          GAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACC                                                                                    <  SRR4302540.3626734/94‑1 (MQ=60)
           AGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCG                                                                                   <  SRR4302540.5829264/94‑1 (MQ=60)
           AGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCG                                                                                   >  SRR4302540.1944515/1‑94 (MQ=60)
           AGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCG                                                                                   >  SRR4302540.8678816/1‑94 (MQ=60)
            GAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGA                                                                                  >  SRR4302540.3504864/1‑94 (MQ=60)
              AAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATT                                                                                >  SRR4302540.6717927/1‑94 (MQ=60)
               AATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTG                                                                               <  SRR4302540.5416407/94‑1 (MQ=60)
                ATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGT                                                                              <  SRR4302540.8616329/94‑1 (MQ=60)
                ATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGT                                                                              <  SRR4302540.526684/94‑1 (MQ=60)
                ATCCGGATGTGGTGTTCCTCGACATCATGCTACCAGGCAACGACGGCATGACCATTTGTCGTGATTTAGGCGCAAAGCGGTCTGGACCGATTGT                                                                              <  SRR4302540.5565768/94‑1 (MQ=60)
                 TCCGGATTTGGTGTTACTCGAGATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTT                                                                             <  SRR4302540.493494/94‑1 (MQ=60)
                  CCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGctt                                                                            >  SRR4302540.8803559/1‑91 (MQ=60)
                    GGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTT                                                                          >  SRR4302540.6690433/1‑94 (MQ=60)
                     GATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTC                                                                         >  SRR4302540.2885896/1‑94 (MQ=60)
                      ATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCT                                                                        <  SRR4302540.7477657/94‑1 (MQ=60)
                       TTTGGTGTTACTCGACATCATGCTGCCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTA                                                                       <  SRR4302540.3941473/94‑1 (MQ=60)
                       TTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTA                                                                       <  SRR4302540.3941474/94‑1 (MQ=60)
                         TGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAAC                                                                     <  SRR4302540.8500338/94‑1 (MQ=60)
                         TGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAAC                                                                     <  SRR4302540.2039207/94‑1 (MQ=60)
                          TGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACC                                                                    >  SRR4302540.795304/1‑94 (MQ=60)
                           GTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCT                                                                   >  SRR4302540.1022042/1‑94 (MQ=60)
                            TGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTC                                                                  >  SRR4302540.3167960/1‑94 (MQ=60)
                            TGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTC                                                                  <  SRR4302540.5814958/94‑1 (MQ=60)
                            TGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTC                                                                  <  SRR4302540.5028575/94‑1 (MQ=60)
                            TGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTC                                                                  <  SRR4302540.9761033/94‑1 (MQ=60)
                              TTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTC                                                                <  SRR4302540.7440928/94‑1 (MQ=60)
                               TACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAATCGGTCTGGACCGATTGCTCTTCTAACCTGTCT                                                               >  SRR4302540.2225937/1‑94 (MQ=60)
                                 CTCGACATCATGCTACCAGGCAAGGACGGCATTACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCG                                                             >  SRR4302540.1735346/1‑94 (MQ=60)
                                 CTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCG                                                             >  SRR4302540.2818347/1‑94 (MQ=60)
                                  TCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGA                                                            <  SRR4302540.6529464/94‑1 (MQ=60)
                                   CGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGAT                                                           <  SRR4302540.6735745/94‑1 (MQ=60)
                                   CGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGAT                                                           >  SRR4302540.6807463/1‑94 (MQ=60)
                                    GACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGTTTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCTATA                                                          >  SRR4302540.9845942/1‑94 (MQ=60)
                                    GACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATA                                                          <  SRR4302540.9005389/94‑1 (MQ=60)
                                    GACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATA                                                          <  SRR4302540.9685823/94‑1 (MQ=60)
                                    GACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATA                                                          >  SRR4302540.9845943/1‑94 (MQ=60)
                                     ACATCATGCTACCAGCCAAGGACGGCATGACCATTTGTCGTGATTTTCGCGCCAAGCGGTCTGGACCGAATGTTCTTCTAACCTCTCTGGATAG                                                         >  SRR4302540.3924579/1‑94 (MQ=60)
                                      CATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGC                                                        >  SRR4302540.41164/1‑94 (MQ=60)
                                      CATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGC                                                        <  SRR4302540.1213899/94‑1 (MQ=60)
                                      CATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGC                                                        <  SRR4302540.4310666/94‑1 (MQ=60)
                                       ATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGGTTGGTCTTCTAttgtctatcgctatcg                                                       >  SRR4302540.4382380/1‑78 (MQ=60)
                                       ATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCG                                                       >  SRR4302540.7699031/1‑94 (MQ=60)
                                       ATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCG                                                       >  SRR4302540.4215604/1‑94 (MQ=60)
                                       ATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCG                                                       >  SRR4302540.4382381/1‑94 (MQ=60)
                                       ATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTATAACCTCTatagatagag                                                       >  SRR4302540.4215603/1‑84 (MQ=60)
                                        TCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGA                                                      >  SRR4302540.2010506/1‑94 (MQ=60)
                                           TGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATAT                                                   <  SRR4302540.323685/94‑1 (MQ=60)
                                           TGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATAT                                                   <  SRR4302540.8853270/94‑1 (MQ=60)
                                            GCTGCCAGGCAAGGACGGCATGACCATTTGGCGTGCTTTACGCGCAAAGCGGTCTGGACCGAGTGTTCTTCTAACCTCTCTCGATAGCGATATG                                                  <  SRR4302540.3083208/94‑1 (MQ=60)
                                             CTACCAGGCAAGGACGGCATGCCCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGA                                                 <  SRR4302540.6089243/94‑1 (MQ=60)
                                             CTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGA                                                 >  SRR4302540.4042492/1‑94 (MQ=60)
                                              TACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAA                                                >  SRR4302540.8603219/1‑94 (MQ=60)
                                              TACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAA                                                <  SRR4302540.2140480/94‑1 (MQ=60)
                                              TACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAA                                                >  SRR4302540.2635105/1‑94 (MQ=60)
                                               ACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAAC                                               >  SRR4302540.9704478/1‑94 (MQ=60)
                                                GCAGGCAAGGACGGCATGACCATTTGTCGATATTTACGCTCAAAGCGGACTGAACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACC                                              <  SRR4302540.6952193/94‑1 (MQ=60)
                                                CCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACC                                              <  SRR4302540.6952192/94‑1 (MQ=60)
                                                CCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACC                                              <  SRR4302540.4754338/94‑1 (MQ=60)
                                                  AGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTACCCTCTCTCGATAGCGATATGAACCAC                                            <  SRR4302540.4166545/94‑1 (MQ=60)
                                                  AGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCAC                                            >  SRR4302540.7248031/1‑94 (MQ=60)
                                                  AGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCAC                                            <  SRR4302540.3170198/94‑1 (MQ=60)
                                                  AGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCAC                                            <  SRR4302540.4166546/94‑1 (MQ=60)
                                                   GGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACA                                           <  SRR4302540.9830025/94‑1 (MQ=60)
                                                    GCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGGTATGAACCACAT                                          <  SRR4302540.2348657/94‑1 (MQ=60)
                                                    GCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACAT                                          >  SRR4302540.2613233/1‑94 (MQ=60)
                                                     CAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATC                                         >  SRR4302540.2419491/1‑94 (MQ=60)
                                                     CAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATC                                         <  SRR4302540.7837773/94‑1 (MQ=60)
                                                      AAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCC                                        >  SRR4302540.7206786/1‑94 (MQ=60)
                                                      AAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCC                                        <  SRR4302540.5624647/94‑1 (MQ=60)
                                                      AAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCC                                        <  SRR4302540.4919107/94‑1 (MQ=60)
                                                      AAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCC                                        <  SRR4302540.4919108/94‑1 (MQ=60)
                                                       AGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCT                                       <  SRR4302540.6648302/94‑1 (MQ=60)
                                                       AGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCT                                       >  SRR4302540.4034616/1‑94 (MQ=60)
                                                       AGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCT                                       >  SRR4302540.1723453/1‑94 (MQ=60)
                                                       AGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCT                                       <  SRR4302540.3648818/94‑1 (MQ=60)
                                                       AGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGATATTCTAACCTCTCTCGATAGCGATATGAACCACATCCT                                       >  SRR4302540.1723454/1‑94 (MQ=60)
                                                         GACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGG                                     <  SRR4302540.9195738/94‑1 (MQ=60)
                                                         GACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGG                                     >  SRR4302540.5131824/1‑94 (MQ=60)
                                                         GACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGG                                     >  SRR4302540.5438370/1‑94 (MQ=60)
                                                         GACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGACAGCGATATGAACCACATCCTGG                                     >  SRR4302540.9074083/1‑94 (MQ=60)
                                                          ACGGCATGACCATTTGTCGTGATTTACGCTCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGC                                    <  SRR4302540.7933767/94‑1 (MQ=60)
                                                          ACGGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGC                                    <  SRR4302540.7933768/94‑1 (MQ=60)
                                                            GGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCAC                                  <  SRR4302540.11292/94‑1 (MQ=60)
                                                            GGCATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCAC                                  <  SRR4302540.4755936/94‑1 (MQ=60)
                                                              CATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTG                                >  SRR4302540.709251/1‑94 (MQ=60)
                                                               ATGACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGG                               >  SRR4302540.7031831/1‑94 (MQ=60)
                                                                TGACCAGTTGTCGTGATTTACGCGCAAAGCGATCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGA                              <  SRR4302540.345994/94‑1 (MQ=60)
                                                                 GACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAA                             >  SRR4302540.6884839/1‑94 (MQ=60)
                                                                  ACCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAA                            <  SRR4302540.461061/94‑1 (MQ=60)
                                                                   CCATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAAT                           <  SRR4302540.8786454/94‑1 (MQ=60)
                                                                    CATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATG                          >  SRR4302540.3892270/1‑94 (MQ=60)
                                                                     ATTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGG                         <  SRR4302540.8087418/94‑1 (MQ=60)
                                                                      TTTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGG                        >  SRR4302540.8832070/1‑94 (MQ=60)
                                                                       TTGTGGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGT                       <  SRR4302540.9523692/94‑1 (MQ=60)
                                                                       TTGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTANCCTCTCTCGATNGCGATATGANCNNNNTCNTGGCACTngnntngnnn                       >  SRR4302540.2254319/1‑84 (MQ=60)
                                                                        TGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTG                      >  SRR4302540.2230521/1‑94 (MQ=60)
                                                                        TGTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTG                      <  SRR4302540.1369134/94‑1 (MQ=60)
                                                                         GTCGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGC                     >  SRR4302540.2062437/1‑94 (MQ=60)
                                                                           CGTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCT                   <  SRR4302540.4305335/94‑1 (MQ=60)
                                                                            GTGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTG                  <  SRR4302540.5982924/94‑1 (MQ=60)
                                                                             TGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGC                 >  SRR4302540.3007389/1‑94 (MQ=60)
                                                                             TGATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGC                 >  SRR4302540.8679585/1‑94 (MQ=60)
                                                                              GATTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCG                <  SRR4302540.6752523/94‑1 (MQ=60)
                                                                               GTTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGA               <  SRR4302540.1301455/94‑1 (MQ=60)
                                                                                TTTACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGAC              >  SRR4302540.4367859/1‑94 (MQ=60)
                                                                                   ACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTAT           <  SRR4302540.2133810/94‑1 (MQ=60)
                                                                                   ACGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTAT           <  SRR4302540.3794004/94‑1 (MQ=60)
                                                                                    CGCGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATA          <  SRR4302540.2469161/94‑1 (MQ=60)
                                                                                      CGCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATT        <  SRR4302540.2288093/94‑1 (MQ=60)
                                                                                       GCAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATTGGTGCCTGCGACTATATTC       >  SRR4302540.785732/1‑94 (MQ=60)
                                                                                        CAAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCT      <  SRR4302540.950413/94‑1 (MQ=60)
                                                                                         AAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGCTATGAACAACATCCGGGCACTGGAAATGGGTGCCTGCGACTATATTCTA     >  SRR4302540.8594341/1‑94 (MQ=60)
                                                                                         AAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTC     <  SRR4302540.6434943/94‑1 (MQ=60)
                                                                                         AAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTC     >  SRR4302540.8594342/1‑94 (MQ=60)
                                                                                         AAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTC     <  SRR4302540.6434942/94‑1 (MQ=60)
                                                                                         AAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTC     <  SRR4302540.6352383/94‑1 (MQ=60)
                                                                                         AAAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTC     <  SRR4302540.2558794/94‑1 (MQ=60)
                                                                                          AAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTGGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCA    >  SRR4302540.8595331/1‑94 (MQ=60)
                                                                                          AAGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCA    <  SRR4302540.7080257/94‑1 (MQ=60)
                                                                                          AAGCGGTCTGGAACGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCA    <  SRR4302540.6714480/94‑1 (MQ=60)
                                                                                           AGCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAA   <  SRR4302540.7145654/94‑1 (MQ=60)
                                                                                            GCGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAA  <  SRR4302540.1136341/94‑1 (MQ=60)
                                                                                             |                                                                                            
ACCATTTTGCGAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAA  >  NC_000913/1680300‑1680485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑