Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,712,350 (T)9→8 intergenic (+55/‑51) ydgR → / → gst putative transport protein/glutathionine S‑transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,712,3420T.98.4% 1121.2 / 7.8 251intergenic (+47/‑59)ydgR/gstputative transport protein/glutathionine S‑transferase
Reads supporting (aligned to +/- strand):  ref base T (4/0);  new base . (136/111);  total (140/111)
Fisher's exact test for biased strand distribution p-value = 1.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 277 total aligned reads displayed.

GCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGC  >  NC_000913/1712250‑1712430
                                                                                            |                                                                                        
 cAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCtt                                                                                         >  1:6236863/1‑93 (MQ=255)
    gATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCttttt                                                                                      >  1:3115648/1‑93 (MQ=255)
     aTGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCtttttt                                                                                     <  2:952776/93‑1 (MQ=255)
      tGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCttttttt                                                                                    <  2:7089078/93‑1 (MQ=255)
      tGACGCAGCACAAAAAGCGGCAAACGCAGCCGTAGCGTAAAATCCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCttttttt                                                                                    <  2:5135161/93‑1 (MQ=255)
           cTGCAGACAAAGCGGGGAAAGCAGCCGTAGCGTAAAATTCAGGGAACCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCttttttttgttc                                                                               <  2:4902310/93‑5 (MQ=255)
            tgaaGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAACCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCa                                                                             <  1:5841242/90‑1 (MQ=255)
             gCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAg                                                                            >  2:4001354/1‑93 (MQ=255)
              cAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGc                                                                           >  1:6470875/1‑93 (MQ=255)
               aaaaaaaGCGGCGAAACCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCa                                                                          <  1:5548698/89‑1 (MQ=255)
                gACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCaa                                                                         >  2:2997537/1‑93 (MQ=255)
                 aCAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAAc                                                                        >  1:1021018/1‑93 (MQ=255)
                 aCAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAAc                                                                        >  1:254138/1‑93 (MQ=255)
                  cAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACg                                                                       <  1:8140209/93‑1 (MQ=255)
                   aaaGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGc                                                                      <  1:1674623/93‑1 (MQ=255)
                    aaGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCa                                                                     >  1:6929563/1‑93 (MQ=255)
                    aaGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCa                                                                     >  2:9145319/1‑93 (MQ=255)
                    aaGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCa                                                                     >  1:2897942/1‑93 (MQ=255)
                     aGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAACCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCAc                                                                    <  1:2690424/93‑1 (MQ=255)
                     aGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCAc                                                                    >  1:153198/1‑93 (MQ=255)
                     aGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCAc                                                                    >  1:5076581/1‑93 (MQ=255)
                     aGCGGCAAAAGCAGCCGTAGCGTAAAATGCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCAc                                                                    <  1:5194409/93‑1 (MQ=255)
                        ggcgAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGATTTTTTTTT‑TTCCGCAACGCACTAc                                                                 >  2:8178672/1‑93 (MQ=255)
                         gagAAAGCAGCCGTAGCGTAAAATTCAGGAAACCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTAcc                                                                <  2:3869554/91‑1 (MQ=255)
                          caaaaGCAGCCTTAGCTAAAAATTCAGGGAACCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCa                                                               <  1:5339317/91‑1 (MQ=255)
                            aaaGCAGCCGTAGCGTAAAATTCAGGGAACCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATa                                                             <  1:7088477/93‑1 (MQ=255)
                            aaaGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATa                                                             >  1:2427343/1‑93 (MQ=255)
                            aaaGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATa                                                             >  2:9707600/1‑93 (MQ=255)
                            aaaGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATa                                                             <  1:4439499/93‑1 (MQ=255)
                             aaGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATAc                                                            <  2:2765407/93‑1 (MQ=255)
                               gcagcCGTAGCGTAAAATTCAGGGAACCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACtt                                                          <  1:4591904/93‑1 (MQ=255)
                                 agcCGTAGCGTAAAATTCAGGAAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTa                                                        <  2:103346/93‑1 (MQ=255)
                                  gctgTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTaa                                                       >  2:6790592/4‑93 (MQ=255)
                                  gcCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTaa                                                       >  2:2043119/1‑93 (MQ=255)
                                   ccGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTaaa                                                      <  1:4617146/93‑1 (MQ=255)
                                   ccGTAGCGTAAAATTAAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTaaa                                                      <  1:8719537/93‑1 (MQ=255)
                                     gTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAAcc                                                    >  1:8347858/1‑93 (MQ=255)
                                      tAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACca                                                   >  1:418591/1‑93 (MQ=255)
                                      tAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACca                                                   >  1:5752181/1‑93 (MQ=255)
                                      tAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACca                                                   >  1:3533036/1‑93 (MQ=255)
                                       aGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACcac                                                  >  1:1096774/1‑93 (MQ=255)
                                        gCGTAAATTTCAGGAAATCTTTTTTAAAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACcaca                                                 <  2:8493973/93‑1 (MQ=255)
                                        gCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACcaca                                                 >  2:3669415/1‑93 (MQ=255)
                                        gCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACcaca                                                 <  1:4835574/93‑1 (MQ=255)
                                        gCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACcaca                                                 >  2:637469/1‑93 (MQ=255)
                                         catAAAATTGAGGAAATCTTTTTTAAAAGCCGCTAACTTTTCGTTAGCGTC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAg                                                <  1:7177601/91‑1 (MQ=255)
                                         cGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAg                                                >  2:2546719/1‑93 (MQ=255)
                                         cGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAg                                                >  1:8904480/1‑93 (MQ=255)
                                          taaaaaTTCAGGAAAACTCTTTCAAAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGa                                               <  2:6320127/91‑1 (MQ=255)
                                          gaaaaaTTAAGGGAATCTTTTTTAAAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGa                                               <  1:6221154/91‑1 (MQ=255)
                                          gTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGa                                               <  2:8619536/93‑1 (MQ=255)
                                          gTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGa                                               <  1:2993265/93‑1 (MQ=255)
                                          gTAAAATTCAGGCACACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCCCAGa                                               <  2:1471028/93‑1 (MQ=255)
                                            aaaaTTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACa                                             >  2:4287777/1‑93 (MQ=255)
                                             aaaTTCAGGGAACCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACaa                                            <  1:9171750/93‑1 (MQ=255)
                                             aaaTTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACaa                                            >  1:8271692/1‑93 (MQ=255)
                                                ttCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAg                                         >  1:8089587/1‑93 (MQ=255)
                                                tcaaGGAAACCTTTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAg                                         <  2:7419594/90‑1 (MQ=255)
                                                 tCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAgg                                        >  1:2615094/1‑93 (MQ=255)
                                                 tCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAgg                                        >  1:7456690/1‑93 (MQ=255)
                                                  cAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGa                                       <  1:6676717/93‑1 (MQ=255)
                                                  cAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGa                                       <  2:9946292/93‑1 (MQ=255)
                                                      gAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTa                                   <  1:1016995/93‑1 (MQ=255)
                                                      gAAACTCTTTTAAAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTa                                   <  1:8535594/93‑1 (MQ=255)
                                                       aacctctTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTAc                                  <  1:7309289/90‑1 (MQ=255)
                                                        acctctTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTAcc                                 <  2:311613/91‑1 (MQ=255)
                                                        aaCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTAcc                                 >  2:9318605/1‑93 (MQ=255)
                                                         aCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCg                                >  1:837463/1‑93 (MQ=255)
                                                           tctTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCCCTACCATACTTTAAACCACAGACAAAAGGAGTTACCGAt                              >  1:3433647/1‑93 (MQ=255)
                                                           tctTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGAt                              >  2:9966352/1‑93 (MQ=255)
                                                           tctTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGAt                              >  2:609947/1‑93 (MQ=255)
                                                             ttttACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGa                            >  2:5704699/1‑93 (MQ=255)
                                                               ttACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGaaa                          <  1:9741124/93‑1 (MQ=255)
                                                                tACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAAt                         <  2:1235382/93‑1 (MQ=255)
                                                                 aCAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAAtt                        <  2:6491650/93‑1 (MQ=255)
                                                                   aaGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTg                       >  1:2222202/1‑93 (MQ=255)
                                                                    aGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGtt                     <  2:6060392/93‑1 (MQ=255)
                                                                    aGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGtt                     >  2:7714641/1‑93 (MQ=255)
                                                                    aGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGtt                     <  2:1708961/93‑1 (MQ=255)
                                                                     gCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAGAAGGAGTTACCGATGAAATTGTTc                    <  2:9649809/93‑1 (MQ=255)
                                                                      ccGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCt                   <  2:407983/93‑1 (MQ=255)
                                                                       cGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTa                  >  1:4274620/1‑93 (MQ=255)
                                                                        gCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTAc                 >  2:10003304/1‑93 (MQ=255)
                                                                        gCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTAc                 <  2:7390399/93‑1 (MQ=255)
                                                                        gCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACAGATGAAATTGCTCTAc                 >  2:2116494/1‑93 (MQ=255)
                                                                          tAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGGAATTGTTCTACaa               >  1:281618/1‑93 (MQ=255)
                                                                           aaCTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACaaa              >  1:6649248/1‑93 (MQ=255)
                                                                               tttCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCg           >  1:9466242/1‑93 (MQ=255)
                                                                                ttCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCggg         >  1:2437699/1‑93 (MQ=255)
                                                                                ttCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCggg         <  2:6611296/93‑1 (MQ=255)
                                                                                ttCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCggg         <  2:8107801/93‑1 (MQ=255)
                                                                                ttCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCggg         <  2:3769304/93‑1 (MQ=255)
                                                                                 tCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGt        >  1:8054762/1‑93 (MQ=255)
                                                                                   gTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGc      >  1:1340637/1‑93 (MQ=255)
                                                                                   gTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGc      <  1:6351900/93‑1 (MQ=255)
                                                                                    ttAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGcc     >  2:3403505/1‑93 (MQ=255)
                                                                                    ttAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGcc     >  1:3340744/1‑93 (MQ=255)
                                                                                      aGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTg   >  1:2093604/1‑93 (MQ=255)
                                                                                       gCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGc  <  2:2867978/93‑1 (MQ=255)
                                                                                       gCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGc  <  2:4650744/93‑1 (MQ=255)
                                                                                            |                                                                                        
GCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGC  >  NC_000913/1712250‑1712430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCC  >  NC_000913/1712257‑1712442
                                                                                             |                                                                                            
GCCGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTG                                                                                              <  SRR4302540.2792062/94‑1 (MQ=60)
    CTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCA                                                                                         <  SRR4302540.8677313/94‑1 (MQ=60)
     agaaaaaaaagcgggaaagGCAGCCGTAGCGAAAACTTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAG                                                                                        <  SRR4302540.3147028/75‑1 (MQ=60)
     TGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAACCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAG                                                                                        <  SRR4302540.8095018/94‑1 (MQ=60)
     TGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAG                                                                                        <  SRR4302540.8210846/94‑1 (MQ=60)
     TGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAG                                                                                        <  SRR4302540.9818061/94‑1 (MQ=60)
     TGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTCAGCGGC‑TTTTTTTTGTTCAG                                                                                        <  SRR4302540.3332342/94‑1 (MQ=60)
     TGCAGACAAAGCGGCGAAACCAGCCGTAGCGTAAAATTCAGGAAACCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAG                                                                                        <  SRR4302540.5951062/94‑1 (MQ=60)
     TGAAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAACCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAG                                                                                        <  SRR4302540.5905835/94‑1 (MQ=60)
     CGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAG                                                                                        <  SRR4302540.581667/94‑1 (MQ=60)
      GCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGC                                                                                       >  SRR4302540.6542872/1‑94 (MQ=60)
        aaaaAAAGCGGCGAAACCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAA                                                                                     <  SRR4302540.5609823/90‑1 (MQ=60)
        AGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAA                                                                                     >  SRR4302540.5114502/1‑94 (MQ=60)
        AGACAAAGCGGCGAAAGCACCCGTAGCGTAAAAGTCAGCGAAACTCTTGTACAAGCACATAACTTTTAGTTAGCGGC‑TATTTTTTGTTCAGCAC                                                                                     >  SRR4302540.5114503/1‑94 (MQ=35)
        AGACAAAGCGGCAAAAGCAGCCGTAGCGTAAAATTCAGGGAACCTTTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAA                                                                                     <  SRR4302540.6302858/94‑1 (MQ=60)
         GACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAAC                                                                                    >  SRR4302540.4687848/1‑94 (MQ=60)
         GACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAAC                                                                                    >  SRR4302540.1031378/1‑94 (MQ=60)
         GACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAAC                                                                                    >  SRR4302540.256596/1‑94 (MQ=60)
         GACAAAGCGGCGAAAGCAGACGTAGCGTAAAATTCAGGGAACCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAAC                                                                                    <  SRR4302540.6962063/94‑1 (MQ=60)
         GACAAAGCGGCAAAGGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAAC                                                                                    <  SRR4302540.281516/94‑1 (MQ=60)
          aaaaaggggaaaaaccagcgGTAGCATAAAATTCAGGGAAACTCTTTCACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACG                                                                                   <  SRR4302540.2811104/74‑1 (MQ=60)
          ACAAAGCGGCAAAAGCAGCCTTAGCTTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACG                                                                                   <  SRR4302540.1042958/94‑1 (MQ=60)
           aaaacgggaaaaaccacccgtgGCGTAAAATCCAGGGAACCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGC                                                                                  <  SRR4302540.8234697/72‑1 (MQ=60)
           CAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGC                                                                                  <  SRR4302540.8234696/94‑1 (MQ=60)
           CAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGC                                                                                  >  SRR4302540.8637861/1‑94 (MQ=60)
           CAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGC                                                                                  >  SRR4302540.7400914/1‑94 (MQ=60)
            AAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCA                                                                                 >  SRR4302540.7006676/1‑94 (MQ=60)
            AAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCA                                                                                 >  SRR4302540.2465861/1‑94 (MQ=60)
            AAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCA                                                                                 >  SRR4302540.2929553/1‑94 (MQ=60)
            AAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCA                                                                                 <  SRR4302540.5486323/94‑1 (MQ=60)
            AAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCA                                                                                 <  SRR4302540.1692818/94‑1 (MQ=60)
            AAAGCGGCAAAAGCAGCCGTAGCGTAAAATTCAGGGAACCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCA                                                                                 <  SRR4302540.1170/94‑1 (MQ=60)
             AAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCAC                                                                                <  SRR4302540.1471096/94‑1 (MQ=60)
             AAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCAC                                                                                >  SRR4302540.154772/1‑94 (MQ=60)
             AAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCAC                                                                                >  SRR4302540.5131468/1‑94 (MQ=60)
             AAGCGGCAAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCAC                                                                                <  SRR4302540.4522385/94‑1 (MQ=60)
              agggggaaaagcagcgaTAGCGTAAATTTCAGGGAACCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACT                                                                               <  SRR4302540.4142375/77‑1 (MQ=60)
              AGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAACCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACT                                                                               <  SRR4302540.2719491/94‑1 (MQ=60)
              AGCGGCAAAAGCAGCCGTAGCGTAAAATGCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACT                                                                               <  SRR4302540.5250974/94‑1 (MQ=60)
                   CAAAAGCAGCCTTAGCTAAAAATTCAGGGAACCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCAT                                                                          <  SRR4302540.5397859/94‑1 (MQ=60)
                    GAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATA                                                                         >  SRR4302540.3205905/1‑94 (MQ=60)
                    GAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATA                                                                         >  SRR4302540.8411051/1‑94 (MQ=60)
                    GAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATA                                                                         >  SRR4302540.8411050/1‑94 (MQ=60)
                    GAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATA                                                                         >  SRR4302540.2453494/1‑94 (MQ=60)
                     AAAGCAGCCGTAGCGTAAAATTCAGGGAACCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATAC                                                                        <  SRR4302540.7167356/94‑1 (MQ=60)
                     AAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATAC                                                                        <  SRR4302540.4487609/94‑1 (MQ=60)
                      AAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACT                                                                       >  SRR4302540.8213983/1‑94 (MQ=60)
                       AGCAGCCGTAGCTTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTT                                                                      <  SRR4302540.2920842/94‑1 (MQ=60)
                        GCGGCCGTAGCGTAAAATTCAGGGAACCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTT                                                                     <  SRR4302540.2041420/94‑1 (MQ=60)
                        GCAGCCGTAGCGTAAAATTCAGGGAACCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTT                                                                     <  SRR4302540.4641321/94‑1 (MQ=60)
                           GCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAA                                                                  <  SRR4302540.3469570/94‑1 (MQ=60)
                            CTGTAGCGAAAAATTCAGGGAACCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAAC                                                                 <  SRR4302540.4666663/94‑1 (MQ=60)
                            CCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTTAGAAATGCACTACCATACTTTAAAC                                                                 >  SRR4302540.706032/1‑94 (MQ=60)
                            CCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAAC                                                                 <  SRR4302540.4666662/94‑1 (MQ=60)
                            CCGTAGCGTAAAATTAAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAAC                                                                 <  SRR4302540.8820774/94‑1 (MQ=60)
                             CGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACC                                                                >  SRR4302540.7315195/1‑94 (MQ=60)
                             CGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACC                                                                >  SRR4302540.4602074/1‑94 (MQ=60)
                             CGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACC                                                                >  SRR4302540.8444744/1‑94 (MQ=60)
                             CGTAGCGTAAAATTCAGCGAACCGCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTCCCATACTTTAAACC                                                                <  SRR4302540.6507758/94‑1 (MQ=60)
                              GTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCCACGCACTACCATCCTTTAAACCA                                                               >  SRR4302540.2994402/1‑94 (MQ=60)
                              GTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCA                                                               >  SRR4302540.5815670/1‑94 (MQ=60)
                              GTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCA                                                               >  SRR4302540.2994400/1‑94 (MQ=60)
                              GTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCA                                                               >  SRR4302540.5647436/1‑94 (MQ=60)
                              GTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCA                                                               >  SRR4302540.422989/1‑94 (MQ=60)
                              GTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCA                                                               >  SRR4302540.3571604/1‑94 (MQ=60)
                              GTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCA                                                               >  SRR4302540.6728405/1‑94 (MQ=60)
                              GTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATAATTTAAACCA                                                               >  SRR4302540.3571605/1‑94 (MQ=60)
                               TAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCAC                                                              >  SRR4302540.1108065/1‑94 (MQ=60)
                                AGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACA                                                             >  SRR4302540.6637151/1‑94 (MQ=60)
                                AGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACA                                                             >  SRR4302540.3495076/1‑94 (MQ=60)
                                 GCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAG                                                            >  SRR4302540.9007575/1‑94 (MQ=60)
                                 GCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAG                                                            <  SRR4302540.4887372/94‑1 (MQ=60)
                                  cataaaattgaggaaatcttTTTTAAAAGCCGCTAACTTTTCGTTAGCGTC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGA                                                           <  SRR4302540.7257612/74‑1 (MQ=60)
                                   GTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGAC                                                          <  SRR4302540.4995237/94‑1 (MQ=60)
                                   GTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGAC                                                          >  SRR4302540.8236184/1‑94 (MQ=60)
                                   GTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGAC                                                          <  SRR4302540.3026262/94‑1 (MQ=60)
                                   GTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGAC                                                          <  SRR4302540.4662247/94‑1 (MQ=60)
                                   GTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑‑TTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACA                                                         <  SRR4302540.8241826/94‑1 (MQ=60)
                                   GTAAAATTAAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTACAGCAACGCACTACCATACTTTAAACCACAGAC                                                          <  SRR4302540.6290362/94‑1 (MQ=60)
                                   GAAAAATTAAGGGAATCTTTTTTAAAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGAC                                                          <  SRR4302540.6290361/94‑1 (MQ=60)
                                     AAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAA                                                        >  SRR4302540.8367794/1‑94 (MQ=60)
                                      AAATTCAGGGAACCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAA                                                       <  SRR4302540.9281771/94‑1 (MQ=60)
                                      AAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAA                                                       >  SRR4302540.2251599/1‑94 (MQ=60)
                                        ATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAG                                                     <  SRR4302540.673058/94‑1 (MQ=60)
                                        ATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAG                                                     >  SRR4302540.8183482/1‑94 (MQ=60)
                                         TTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGG                                                    >  SRR4302540.8502241/1‑94 (MQ=60)
                                         TTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGG                                                    >  SRR4302540.2643342/1‑94 (MQ=60)
                                         TTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGG                                                    >  SRR4302540.7539827/1‑94 (MQ=60)
                                          ccagggaccCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGA                                                   <  SRR4302540.2555566/85‑1 (MQ=60)
                                           CAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAG                                                  <  SRR4302540.6750472/94‑1 (MQ=60)
                                            AGGGAACCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGT                                                 <  SRR4302540.7171868/94‑1 (MQ=60)
                                            AGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGT                                                 >  SRR4302540.8871831/1‑94 (MQ=60)
                                               GAATCTCTTTGACAAGCCGCTAACTTTTCGTTAGCGGC‑TTGTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTAC                                              <  SRR4302540.2184813/94‑1 (MQ=60)
                                               GAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTAC                                              >  SRR4302540.9322628/1‑94 (MQ=60)
                                               GAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTAC                                              <  SRR4302540.1027301/94‑1 (MQ=60)
                                               GAAACTCTTTTAAAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTAC                                              <  SRR4302540.8634763/94‑1 (MQ=60)
                                                AATCTTTTTTACAAGCCGCTAACTTTTCTTTAGCGGC‑TTTTTGTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACC                                             <  SRR4302540.4998784/94‑1 (MQ=60)
                                                AACCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACC                                             <  SRR4302540.5428020/94‑1 (MQ=60)
                                                AACCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACC                                             <  SRR4302540.7390889/94‑1 (MQ=60)
                                                AAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACC                                             >  SRR4302540.267905/1‑94 (MQ=60)
                                                 AACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCG                                            >  SRR4302540.846354/1‑94 (MQ=60)
                                                 AACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGATCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACAG                                            >  SRR4302540.846671/1‑94 (MQ=60)
                                                   CTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTT‑TTCAGCAATGCTCTACCATACTTTAAACCACAGACATAAGTAGTTACCGAT                                          >  SRR4302540.9051471/1‑94 (MQ=60)
                                                   CTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCCCTACCATACTTTAAACCACAGACAAAAGGAGTTACCGAT                                          >  SRR4302540.3470975/1‑94 (MQ=60)
                                                   CTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGAT                                          >  SRR4302540.4653707/1‑94 (MQ=60)
                                                   CTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGAT                                          >  SRR4302540.6822129/1‑94 (MQ=60)
                                                   CTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGAT                                          >  SRR4302540.5371281/1‑94 (MQ=60)
                                                    TCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCCACTCACTACCATACTCTAAACCACAGACAAAATGAGTTACCGATG                                         >  SRR4302540.4200882/1‑94 (MQ=60)
                                                    TCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATG                                         >  SRR4302540.4311276/1‑94 (MQ=60)
                                                    TCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATG                                         >  SRR4302540.4311278/1‑94 (MQ=60)
                                                    TCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTGACCGATG                                         >  SRR4302540.4738332/1‑94 (MQ=60)
                                                       TTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACTATAGGTGTTACCGATGAAA                                      >  SRR4302540.9073301/1‑94 (MQ=60)
                                                        TTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAAT                                     <  SRR4302540.9855471/94‑1 (MQ=60)
                                                        TTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAAT                                     <  SRR4302540.6379292/94‑1 (MQ=60)
                                                           CAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTG                                   >  SRR4302540.2246157/1‑94 (MQ=60)
                                                           CAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGT                                  >  SRR4302540.1547269/1‑94 (MQ=60)
                                                            AAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTT                                 >  SRR4302540.1030312/1‑94 (MQ=60)
                                                            AAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTT                                 >  SRR4302540.2455872/1‑94 (MQ=60)
                                                             AGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTC                                <  SRR4302540.9941499/94‑1 (MQ=60)
                                                              GCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCT                               <  SRR4302540.6558834/94‑1 (MQ=60)
                                                              GCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCT                               >  SRR4302540.2249270/1‑94 (MQ=60)
                                                              GCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCT                               <  SRR4302540.5369135/94‑1 (MQ=60)
                                                               CCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTA                              >  SRR4302540.4321112/1‑94 (MQ=60)
                                                                CGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTAC                             <  SRR4302540.8987689/94‑1 (MQ=60)
                                                                 GCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACA                            >  SRR4302540.10063681/1‑94 (MQ=60)
                                                                 GCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACA                            >  SRR4302540.1210804/1‑94 (MQ=60)
                                                                 GCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACA                            <  SRR4302540.3549818/94‑1 (MQ=60)
                                                                  CTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGGAATTGTTCTACAA                           >  SRR4302540.284383/1‑94 (MQ=60)
                                                                  CTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAA                           <  SRR4302540.9634833/94‑1 (MQ=60)
                                                                  CTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAA                           <  SRR4302540.2635720/94‑1 (MQ=60)
                                                                  CTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAA                           >  SRR4302540.2675191/1‑94 (MQ=60)
                                                                   TAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAA                          >  SRR4302540.6722803/1‑94 (MQ=60)
                                                                   TAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAA                          >  SRR4302540.2222255/1‑94 (MQ=60)
                                                                     ACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACC                        >  SRR4302540.8462035/1‑94 (MQ=60)
                                                                      CTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCG                       >  SRR4302540.1672699/1‑94 (MQ=60)
                                                                      CTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCG                       >  SRR4302540.4383685/1‑94 (MQ=60)
                                                                      CGTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCG                       <  SRR4302540.5246585/94‑1 (MQ=60)
                                                                       TTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCG                       >  SRR4302540.9578540/1‑94 (MQ=60)
                                                                       TTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTTTGCAAACCGG                      >  SRR4302540.2589176/1‑94 (MQ=60)
                                                                       TTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGG                      >  SRR4302540.740361/1‑94 (MQ=60)
                                                                        TTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGG                     >  SRR4302540.7152943/1‑94 (MQ=60)
                                                                        TTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGG                     >  SRR4302540.2463948/1‑94 (MQ=60)
                                                                         TTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGT                    <  SRR4302540.308991/94‑1 (MQ=60)
                                                                         TTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGT                    >  SRR4302540.1081171/1‑94 (MQ=60)
                                                                         TTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGT                    >  SRR4302540.8148299/1‑94 (MQ=60)
                                                                           CGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGC                  >  SRR4302540.1354676/1‑94 (MQ=60)
                                                                            GTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCC                 >  SRR4302540.7358521/1‑94 (MQ=60)
                                                                            GTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCC                 >  SRR4302540.3377410/1‑94 (MQ=60)
                                                                            GTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCC                 >  SRR4302540.6042091/1‑94 (MQ=60)
                                                                            GTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCC                 <  SRR4302540.6422640/94‑1 (MQ=60)
                                                                              TAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTG               >  SRR4302540.2116083/1‑94 (MQ=60)
                                                                               AGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGC              >  SRR4302540.1988713/1‑94 (MQ=60)
                                                                                  GGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCT           >  SRR4302540.734381/1‑94 (MQ=60)
                                                                                  GGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCT           >  SRR4302540.9031782/1‑94 (MQ=60)
                                                                                     CTTTTTTTTGTTCAGGAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCT         <  SRR4302540.5205549/94‑1 (MQ=60)
                                                                                     CTTTTTTTTGTTCAGCAACGCGCTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGATCTACAAACCGGGTGCCTGCTCTCT         >  SRR4302540.1409487/1‑94 (MQ=60)
                                                                                     CTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCT         <  SRR4302540.1382660/94‑1 (MQ=60)
                                                                                     CTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCT         <  SRR4302540.1382659/94‑1 (MQ=60)
                                                                                     CTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCT         <  SRR4302540.7833825/94‑1 (MQ=60)
                                                                                     CTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCT         >  SRR4302540.1409485/1‑94 (MQ=60)
                                                                                     CTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCATGCTCTCT         >  SRR4302540.1409484/1‑94 (MQ=60)
                                                                                      TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTC        >  SRR4302540.9984922/1‑94 (MQ=60)
                                                                                      TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTC        <  SRR4302540.249040/94‑1 (MQ=60)
                                                                                      TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTC        <  SRR4302540.291989/94‑1 (MQ=60)
                                                                                      TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTC        <  SRR4302540.291995/94‑1 (MQ=60)
                                                                                       TTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCG       <  SRR4302540.7330648/94‑1 (MQ=60)
                                                                                        TTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGCCAAAAGGAGTTACCGATGAGATTGTTCTACCAACCGGGTGacagccatctgct      >  SRR4302540.5428781/1‑81 (MQ=60)
                                                                                        TTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGC      >  SRR4302540.5429064/1‑94 (MQ=60)
                                                                                         TTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCT     <  SRR4302540.5179603/94‑1 (MQ=60)
                                                                                         TTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCT     <  SRR4302540.10044971/94‑1 (MQ=60)
                                                                                          TTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTT    >  SRR4302540.1898131/1‑94 (MQ=60)
                                                                                          TTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTT    >  SRR4302540.7404416/1‑94 (MQ=60)
                                                                                          TTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTT    >  SRR4302540.7404415/1‑94 (MQ=60)
                                                                                          TTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACAGATGAAATTGTTCTACAAAGCGGGTGCCTGCTCTCTCGatc    >  SRR4302540.1898132/1‑91 (MQ=60)
                                                                                            TTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCC  <  SRR4302540.5120233/94‑1 (MQ=60)
                                                                                             |                                                                                            
GACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCC  >  NC_000913/1712257‑1712442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑