Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,712,350 (T)9→8 intergenic (+55/‑51) ydgR → / → gst putative transport protein/glutathionine S‑transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,712,3420T.99.4% 776.4 / ‑2.7 169intergenic (+47/‑59)ydgR/gstputative transport protein/glutathionine S‑transferase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base . (73/95);  total (74/95)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 198 total aligned reads displayed.

GCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGC  >  NC_000913/1712250‑1712430
                                                                                            |                                                                                        
 cAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCtt                                                                                         <  1:1473424/93‑1 (MQ=255)
  aGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCttt                                                                                        <  2:1201141/93‑1 (MQ=255)
  aGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCttt                                                                                        <  1:6212833/93‑1 (MQ=255)
  aGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCttt                                                                                        >  1:1539633/1‑93 (MQ=255)
  aGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCttt                                                                                        <  1:5162297/93‑1 (MQ=255)
  aGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCttt                                                                                        >  1:1887118/1‑93 (MQ=255)
   ggATGACGCTGCAGACAAAGCGTCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCtttt                                                                                       >  1:1143615/1‑93 (MQ=255)
   ggATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCtttt                                                                                       >  2:5433553/1‑93 (MQ=255)
      tGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCttttttt                                                                                    >  2:1392519/1‑93 (MQ=255)
        aCGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCttttttttt                                                                                  >  1:4293864/1‑93 (MQ=255)
        aCGCGGCAGAAAAGGCGGCGAAAGCAGCGGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCttttttttg                                                                                  <  1:2694031/93‑2 (MQ=255)
         cGCTGCAAACAAAGCGGCGAAAGCAGCCGTAGCTTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCttttttttgt                                                                                 <  1:4148696/93‑3 (MQ=255)
           cTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCttttttttgttt                                                                               >  1:4772599/1‑89 (MQ=255)
           cTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCttttttttgttc                                                                               <  2:2638070/93‑5 (MQ=255)
            tGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCa                                                                             >  2:2829961/1‑93 (MQ=255)
             gaaGACAAAGGGGCAAAAGCAGCCGTAGCGAAAAATTCAGGGAACCTTTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAg                                                                            <  2:4460691/91‑1 (MQ=255)
               ggACAAGGGGTCGAAGGCAGGCGTAGCGTAAAATTCAGGGAATCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCa                                                                          <  1:453998/92‑1 (MQ=255)
               aGACAAGGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCa                                                                          <  1:3215627/93‑1 (MQ=255)
               aGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCa                                                                          >  1:3017802/1‑93 (MQ=255)
                gACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCaa                                                                         >  1:2655057/1‑93 (MQ=255)
                gACAAAGCCGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCaa                                                                         >  2:2919254/1‑93 (MQ=255)
                aaCAAAGGGGCGAAAGCAGCAGTAGCGTAAAATTCAGGGAAACTCTTTAACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCaa                                                                         <  1:1668633/92‑1 (MQ=255)
                   aaaGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGc                                                                      >  2:780618/1‑93 (MQ=255)
                   aaaGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGc                                                                      >  2:2552470/1‑93 (MQ=255)
                    aaGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCa                                                                     >  2:2965627/1‑93 (MQ=255)
                    aaGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCa                                                                     >  1:3165994/1‑93 (MQ=255)
                     aGCGGAAAAAGCAGCCGTAGCGTAAAATTCAGGGAACCTCTTTTACAAGCCGCTACCTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCAc                                                                    <  2:2705405/93‑1 (MQ=255)
                      gcggcgAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCAcc                                                                   >  1:3900635/1‑92 (MQ=255)
                        ggcgAAAGCAGCCGTAGCGTAAAATTCAGGGAAACGCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTAc                                                                 <  1:527473/93‑1 (MQ=255)
                           ggaaGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCAt                                                              >  2:6028553/3‑93 (MQ=255)
                            aaaGCAGCCGTAGCGTAAAATTCAGGGAATCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATa                                                             <  2:4773960/93‑1 (MQ=255)
                            aaaGCAGCCGTAGCGTAAAATTCAGGGAACCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATa                                                             <  2:3089757/93‑1 (MQ=255)
                            aaaGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATa                                                             >  2:1715457/1‑93 (MQ=255)
                            aaaGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATa                                                             >  1:56420/1‑93 (MQ=255)
                            aaaGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATa                                                             >  1:5482093/1‑93 (MQ=255)
                             aaGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATAc                                                            <  1:3852506/93‑1 (MQ=255)
                             aaGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATAc                                                            <  2:1572537/93‑1 (MQ=255)
                                 agcCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTa                                                        >  1:3789961/1‑93 (MQ=255)
                                 acccGTAGAGTAAAATTAAGGGAACCTTTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTa                                                        <  1:3630947/91‑1 (MQ=255)
                                  gcCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTaa                                                       >  2:3650846/1‑93 (MQ=255)
                                  gcCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTaa                                                       >  2:360271/1‑93 (MQ=255)
                                   gcGTAGCGAAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTaaa                                                      <  1:1616026/92‑1 (MQ=255)
                                   ccGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGGCACGCACTACCATACTTTaaa                                                      >  2:4202351/1‑93 (MQ=255)
                                   ccGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTaaa                                                      >  2:2305480/1‑93 (MQ=255)
                                     gTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAAcc                                                    <  1:3733101/93‑1 (MQ=255)
                                     gTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAAcc                                                    <  1:6138447/93‑1 (MQ=255)
                                     gTAGCGTAAAATTAAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAAcc                                                    <  1:447839/93‑1 (MQ=255)
                                     gTAGCCTAAAATACAGGGAAACTCTTTTCCAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTGCCATACTTTAAAcc                                                    <  1:608233/93‑1 (MQ=255)
                                      tAGCGTAAAATTCAGGGAACCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACca                                                   <  2:5463267/93‑1 (MQ=255)
                                      tAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAAATTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACca                                                   >  1:8242/1‑93 (MQ=255)
                                        gcatAAAATTCGGGAAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACcaca                                                 <  2:1273435/90‑1 (MQ=255)
                                        gCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACcaca                                                 <  1:2504639/93‑1 (MQ=255)
                                         caaaaaaTTCAGGAAAGCTTTTTTAAAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAg                                                <  2:2436928/90‑1 (MQ=255)
                                          gaaaaaTTCAGGAAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGa                                               <  1:2017339/91‑1 (MQ=255)
                                          gTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGa                                               >  1:6041552/1‑93 (MQ=255)
                                              aaTTTAGGGAAACCTTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACaaa                                           <  1:5035102/93‑1 (MQ=255)
                                                ttCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAg                                         <  1:812859/93‑1 (MQ=255)
                                                 tCAGGAAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAgg                                        <  1:2429344/93‑1 (MQ=255)
                                                  cAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGa                                       >  2:4901452/1‑93 (MQ=255)
                                                    gggAACCTCTTTAACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGt                                     <  2:191164/93‑1 (MQ=255)
                                                    gggAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGt                                     >  1:6004806/1‑93 (MQ=255)
                                                    gggAAACTCTTTTAAAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGt                                     <  1:3415606/93‑1 (MQ=255)
                                                     ggAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGtt                                    <  1:4668956/93‑1 (MQ=255)
                                                     gaaaaCTCTTTTAAAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGtt                                    <  1:2310139/91‑1 (MQ=255)
                                                      gAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTa                                   >  2:5650891/1‑93 (MQ=255)
                                                      gAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTa                                   >  2:284926/1‑93 (MQ=255)
                                                       aaaCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTAc                                  >  1:6016656/1‑93 (MQ=255)
                                                       aaaCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTAc                                  >  1:1494831/1‑93 (MQ=255)
                                                       aaaCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTAc                                  <  1:5841204/93‑1 (MQ=255)
                                                         aCTTTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCg                                <  1:4798536/93‑1 (MQ=255)
                                                          ctctTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGa                               >  1:2555122/1‑93 (MQ=255)
                                                           tctTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGAt                              >  1:6272458/1‑93 (MQ=255)
                                                             ttttACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGa                            >  2:5366591/1‑93 (MQ=255)
                                                              tttAAAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGaa                           <  1:895079/93‑1 (MQ=255)
                                                               ttACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGaaa                          <  2:5139183/93‑1 (MQ=255)
                                                                tACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAAt                         >  1:1116483/1‑93 (MQ=255)
                                                                tACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAAt                         >  2:179838/1‑93 (MQ=255)
                                                                  cacgCCGCTAACTTTGCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTg                       <  2:2235166/90‑1 (MQ=255)
                                                                    aGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGtt                     >  2:214976/1‑93 (MQ=255)
                                                                      ccGCTAACTTTTCGTTATCCGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCt                   <  2:1892818/93‑1 (MQ=255)
                                                                      ccGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCt                   <  2:3930638/93‑1 (MQ=255)
                                                                      ccGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCt                   <  1:1773272/93‑1 (MQ=255)
                                                                      ccGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCt                   <  2:4585180/93‑1 (MQ=255)
                                                                       cGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTa                  >  2:5227533/1‑93 (MQ=255)
                                                                         cTAGCTTTTCTTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACa                <  1:2944326/93‑1 (MQ=255)
                                                                         cTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACa                <  1:1008992/93‑1 (MQ=255)
                                                                         cTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACa                <  2:622436/93‑1 (MQ=255)
                                                                          tAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACaa               >  1:2871995/1‑93 (MQ=255)
                                                                          tAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACaa               >  1:5875194/1‑93 (MQ=255)
                                                                          tAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACaa               >  1:4105921/1‑93 (MQ=255)
                                                                           aaCTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACaaa              >  1:5553591/1‑93 (MQ=255)
                                                                             cTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAAcc            >  2:3343622/1‑93 (MQ=255)
                                                                              ttttCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCg           >  1:4928866/1‑93 (MQ=255)
                                                                              ttttCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCg           >  1:1988366/1‑93 (MQ=255)
                                                                              ttttCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCg           >  1:3370685/1‑93 (MQ=255)
                                                                               tttCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCgg          <  2:3054011/93‑1 (MQ=255)
                                                                               tttCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCgg          <  2:5735502/93‑1 (MQ=255)
                                                                                    ttAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGcc     >  1:308036/1‑93 (MQ=255)
                                                                                       gCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGc  >  1:1813189/1‑93 (MQ=255)
                                                                                       gCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGc  <  1:1018496/93‑1 (MQ=255)
                                                                                            |                                                                                        
GCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGC  >  NC_000913/1712250‑1712430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCC  >  NC_000913/1712257‑1712443
                                                                                             |                                                                                             
GACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTT                                                                                               >  SRR4302533.4319689/1‑94 (MQ=60)
  CGCTGCAAACAAAGCGGCGAAAGCAGCCGTAGCTTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTT                                                                                            <  SRR4302533.4173833/94‑1 (MQ=60)
    CTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCA                                                                                          >  SRR4302533.4998874/1‑94 (MQ=60)
     TGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAG                                                                                         >  SRR4302533.6089321/1‑94 (MQ=60)
       CAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCA                                                                                       >  SRR4302533.3036258/1‑94 (MQ=60)
        ggacaaggggtcgaaggcaggCGTAGCGTAAAATTCAGGGAATCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAA                                                                                      <  SRR4302533.456713/73‑1 (MQ=60)
        AGACAAGGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAA                                                                                      <  SRR4302533.3235451/94‑1 (MQ=60)
        AGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAA                                                                                      >  SRR4302533.2671365/1‑94 (MQ=60)
        AGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAA                                                                                      >  SRR4302533.4913716/1‑94 (MQ=60)
         GAAAAAGGGGCGAAAGCAGCCGTAGCTTAAAATTCAGGAAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAAC                                                                                     <  SRR4302533.3396715/94‑1 (MQ=60)
         AACAAAGGGGCGAAAGCAGCAGTAGCGTAAAATTCAGGGAAACTCTTTAACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAAC                                                                                     <  SRR4302533.1678714/94‑1 (MQ=60)
            AAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCA                                                                                  >  SRR4302533.3185476/1‑94 (MQ=60)
              AGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACC                                                                                >  SRR4302533.3924445/1‑94 (MQ=60)
                GGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAATCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTAC                                                                              <  SRR4302533.5325588/94‑1 (MQ=60)
                CGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTAC                                                                              >  SRR4302533.4033146/1‑94 (MQ=60)
                 GGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACC                                                                             >  SRR4302533.3222248/1‑94 (MQ=60)
                 GGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACGCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACC                                                                             <  SRR4302533.530608/94‑1 (MQ=60)
                   CGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCAT                                                                           >  SRR4302533.82608/1‑94 (MQ=60)
                    GAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATA                                                                          <  SRR4302533.588802/94‑1 (MQ=60)
                    GAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATA                                                                          >  SRR4302533.56796/1‑94 (MQ=60)
                    GAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATA                                                                          >  SRR4302533.5515142/1‑94 (MQ=60)
                    AAAAGCAGCCGTAGCGTAAAATTCAGGAAACCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATA                                                                          <  SRR4302533.2981957/94‑1 (MQ=60)
                      AAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTtttagcaacccccttaccatct                                                                         >  SRR4302533.2729075/1‑72 (MQ=60)
                      AAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACT                                                                        <  SRR4302533.189983/94‑1 (MQ=60)
                      AAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACT                                                                        <  SRR4302533.3876033/94‑1 (MQ=60)
                         CAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTA                                                                     >  SRR4302533.3813168/1‑94 (MQ=60)
                          ACCCGTAGAGTAAAATTAAGGGAACCTTTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAA                                                                    <  SRR4302533.3653286/94‑1 (MQ=60)
                            cgctagccTAAAATTCAGGGAAACTCTTTTCCAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAAC                                                                  <  SRR4302533.6254761/86‑1 (MQ=60)
                            GCGTAGCGAAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAAC                                                                  <  SRR4302533.1625821/94‑1 (MQ=60)
                              GTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCA                                                                <  SRR4302533.6175424/94‑1 (MQ=60)
                              GTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCA                                                                <  SRR4302533.3756003/94‑1 (MQ=60)
                              GTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAAATTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCA                                                                >  SRR4302533.8315/1‑94 (MQ=60)
                              GTAGCGTAAAATTAAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCA                                                                <  SRR4302533.450515/94‑1 (MQ=60)
                              GTAGCCTAAAATACAGGGAAACTCTTTTCCAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTGCCATACTTTAAACCA                                                                <  SRR4302533.611810/94‑1 (MQ=60)
                                AGCGTAAAATTCAGGAAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACA                                                              <  SRR4302533.6166168/94‑1 (MQ=60)
                                 GCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAG                                                             <  SRR4302533.2520080/94‑1 (MQ=60)
                                 GCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAG                                                             >  SRR4302533.1137078/1‑94 (MQ=60)
                                  CGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGA                                                            >  SRR4302533.6078160/1‑94 (MQ=60)
                                   GAAAAATTCAGGAAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGAC                                                           <  SRR4302533.2029770/94‑1 (MQ=60)
                                     AAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAA                                                         <  SRR4302533.1882005/94‑1 (MQ=60)
                                       AATTTAGGGAAACCTTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAA                                                       <  SRR4302533.5065599/94‑1 (MQ=60)
                                        ATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAG                                                      >  SRR4302533.3036390/1‑94 (MQ=60)
                                         TTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGG                                                     >  SRR4302533.2664731/1‑94 (MQ=60)
                                         TTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGG                                                     <  SRR4302533.2029632/94‑1 (MQ=60)
                                         TTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGG                                                     <  SRR4302533.817618/94‑1 (MQ=60)
                                          TCAGGAAACCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGA                                                    <  SRR4302533.232601/94‑1 (MQ=60)
                                          TCAGGAAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGA                                                    <  SRR4302533.3553275/94‑1 (MQ=60)
                                          TCAGGAAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGA                                                    <  SRR4302533.2444361/94‑1 (MQ=60)
                                           CAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACTCCAGACAAAAGGAG                                                   >  SRR4302533.5648616/1‑94 (MQ=60)
                                           CAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAG                                                   <  SRR4302533.2694524/94‑1 (MQ=60)
                                            AGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGT                                                  >  SRR4302533.6041189/1‑94 (MQ=60)
                                            AGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGT                                                  <  SRR4302533.2712244/94‑1 (MQ=60)
                                             GGGAACCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTT                                                 <  SRR4302533.4789190/94‑1 (MQ=60)
                                             GGGAAACTCTTTTAAAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTT                                                 <  SRR4302533.3436711/94‑1 (MQ=60)
                                             GGAAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTT                                                 <  SRR4302533.88118/94‑1 (MQ=60)
                                              GGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTA                                                <  SRR4302533.4697248/94‑1 (MQ=60)
                                              GAAAACTCTTTTAAAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTA                                                <  SRR4302533.2324450/94‑1 (MQ=60)
                                               GAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTAC                                               >  SRR4302533.6053112/1‑94 (MQ=60)
                                               GAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTAC                                               >  SRR4302533.1503838/1‑94 (MQ=60)
                                                AACCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACC                                              <  SRR4302533.1523308/94‑1 (MQ=60)
                                                AAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACC                                              >  SRR4302533.5066693/1‑94 (MQ=60)
                                                AAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACC                                              <  SRR4302533.5876521/94‑1 (MQ=60)
                                                 ACCTCTTTTAAAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCG                                             <  SRR4302533.2550602/94‑1 (MQ=60)
                                                  attttttaaaAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGA                                            <  SRR4302533.3265729/84‑1 (MQ=60)
                                                  ACTTTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGA                                            <  SRR4302533.4827602/94‑1 (MQ=60)
                                                  ACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGA                                            <  SRR4302533.3156769/94‑1 (MQ=60)
                                                  ACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGA                                            >  SRR4302533.2570840/1‑94 (MQ=60)
                                                   CTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGAT                                           >  SRR4302533.6309925/1‑94 (MQ=60)
                                                     CTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGA                                         >  SRR4302533.601104/1‑94 (MQ=60)
                                                     CTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGA                                         >  SRR4302533.1098064/1‑94 (MQ=60)
                                                      TTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAA                                        >  SRR4302533.4627569/1‑94 (MQ=60)
                                                      TTTTAAAAGCCTCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAA                                        <  SRR4302533.3694649/94‑1 (MQ=60)
                                                       TTTAAAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAA                                       <  SRR4302533.900337/94‑1 (MQ=60)
                                                        TTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAAT                                      >  SRR4302533.1123109/1‑94 (MQ=60)
                                                           CAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGT                                   >  SRR4302533.1188232/1‑94 (MQ=60)
                                                           CAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGT                                   >  SRR4302533.5807044/1‑94 (MQ=60)
                                                            AAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTT                                  <  SRR4302533.1822340/94‑1 (MQ=60)
                                                               CCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTA                               <  SRR4302533.1661767/94‑1 (MQ=60)
                                                               CCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTA                               <  SRR4302533.1784073/94‑1 (MQ=60)
                                                                CGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTAC                              >  SRR4302533.4353543/1‑94 (MQ=60)
                                                                 GCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACA                             <  SRR4302533.1549787/94‑1 (MQ=60)
                                                                  CTAGCTTTTCTTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAA                            <  SRR4302533.2962295/94‑1 (MQ=60)
                                                                  CTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAA                            >  SRR4302533.4130890/1‑94 (MQ=60)
                                                                  CTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAA                            >  SRR4302533.5910711/1‑94 (MQ=60)
                                                                  CTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAA                            <  SRR4302533.1014896/94‑1 (MQ=60)
                                                                  CTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAA                            >  SRR4302533.2889666/1‑94 (MQ=60)
                                                                   TAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAA                           >  SRR4302533.5587128/1‑94 (MQ=60)
                                                                    AACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAAC                          <  SRR4302533.2922020/94‑1 (MQ=60)
                                                                    AACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAAC                          <  SRR4302533.4823150/94‑1 (MQ=60)
                                                                    AACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAAC                          <  SRR4302533.60584/94‑1 (MQ=60)
                                                                    AACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAAC                          <  SRR4302533.3834702/94‑1 (MQ=60)
                                                                    AACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAAC                          <  SRR4302533.4528400/94‑1 (MQ=60)
                                                                      CTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCG                        <  SRR4302533.3781410/94‑1 (MQ=60)
                                                                      CTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCG                        >  SRR4302533.4958712/1‑94 (MQ=60)
                                                                      CTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCG                        >  SRR4302533.1497133/1‑94 (MQ=60)
                                                                      CTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCG                        >  SRR4302533.2000531/1‑94 (MQ=60)
                                                                      CTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCG                        >  SRR4302533.3391501/1‑94 (MQ=60)
                                                                       TTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGG                       <  SRR4302533.5568825/94‑1 (MQ=60)
                                                                       TTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGG                       <  SRR4302533.3882934/94‑1 (MQ=60)
                                                                        TTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGG                      <  SRR4302533.1700018/94‑1 (MQ=60)
                                                                        TTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGG                      <  SRR4302533.4302107/94‑1 (MQ=60)
                                                                        TTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGG                      <  SRR4302533.2490934/94‑1 (MQ=60)
                                                                            GTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCC                  >  SRR4302533.309922/1‑94 (MQ=60)
                                                                              TAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTG                <  SRR4302533.4138907/94‑1 (MQ=60)
                                                                              TAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTG                <  SRR4302533.6210610/94‑1 (MQ=60)
                                                                               AGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGC               >  SRR4302533.1824261/1‑94 (MQ=60)
                                                                                GCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCT              <  SRR4302533.1024485/94‑1 (MQ=60)
                                                                                 CGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTC             <  SRR4302533.2727238/94‑1 (MQ=60)
                                                                                 CGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTC             >  SRR4302533.6093475/1‑94 (MQ=60)
                                                                                 CGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTC             >  SRR4302533.2493071/1‑94 (MQ=60)
                                                                                 CGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTC             >  SRR4302533.483690/1‑94 (MQ=60)
                                                                                    gcTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTC           <  SRR4302533.2033416/92‑1 (MQ=60)
                                                                                     CTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCT          >  SRR4302533.2286543/1‑94 (MQ=60)
                                                                                     CTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCT          >  SRR4302533.3839844/1‑94 (MQ=60)
                                                                                      TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTC         >  SRR4302533.4172557/1‑94 (MQ=60)
                                                                                        TTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGC       >  SRR4302533.2065269/1‑94 (MQ=60)
                                                                                         TTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCT      >  SRR4302533.3777405/1‑94 (MQ=60)
                                                                                         TTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCT      <  SRR4302533.18159/94‑1 (MQ=60)
                                                                                          TTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTT     >  SRR4302533.53072/1‑94 (MQ=60)
                                                                                             TGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCC  <  SRR4302533.4516897/94‑1 (MQ=60)
                                                                                             TGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCC  <  SRR4302533.5277875/94‑1 (MQ=60)
                                                                                             |                                                                                             
GACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCC  >  NC_000913/1712257‑1712443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑