Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,716,150	A→G	T88T (ACT→ACC)	ydhA ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,716,150	0	A	G	99.2%	839.8 / ‑5.1	265	T88T (ACT→ACC)	ydhA	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/2); new base G (162/101); total (162/103)
Fisher's exact test for biased strand distribution p-value = 1.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 278 total aligned reads displayed.

GACGCTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACAGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAG  >  NC_000913/1716059‑1716239

 aCGCTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGt                                                                                         <  1:5792793/93‑1 (MQ=255)

  cGCTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTc                                                                                        >  1:4557994/1‑93 (MQ=255)

   gCTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCg                                                                                       >  2:1047339/1‑93 (MQ=255)

    cTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGc                                                                                      >  2:4510732/1‑93 (MQ=255)

    cTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGc                                                                                      <  1:1938441/93‑1 (MQ=255)

     tATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCt                                                                                     >  1:2625129/1‑93 (MQ=255)

     tATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCt                                                                                     <  2:8026705/93‑1 (MQ=255)

      aTTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCtt                                                                                    <  2:3455917/93‑1 (MQ=255)

       ttGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTc                                                                                   >  2:3294723/1‑93 (MQ=255)

        tgtgCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGATAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCa                                                                                  >  2:3724039/1‑93 (MQ=255)

        tgtgCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCa                                                                                  <  1:6277276/93‑1 (MQ=255)

          tgCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATc                                                                                <  2:862447/93‑1 (MQ=255)

          gccgccgcCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATc                                                                                <  2:436592/91‑1 (MQ=255)

             gccgccCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGcc                                                                             >  2:5659182/1‑93 (MQ=255)

             gccgccCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGcc                                                                             <  2:4562465/93‑1 (MQ=255)

              ccgccCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTGAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCt                                                                            >  1:1918022/1‑93 (MQ=255)

               cgccCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCtt                                                                           <  2:7653955/93‑1 (MQ=255)

               cgccCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCtt                                                                           >  2:6566871/1‑93 (MQ=255)

               cgccCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCtt                                                                           >  1:9421253/1‑93 (MQ=255)

               cgccCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCtt                                                                           >  1:9037092/1‑93 (MQ=255)

                cccctTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCttt                                                                          <  2:5671687/92‑1 (MQ=255)

                 ccccTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTc                                                                         <  1:8105756/93‑1 (MQ=255)

                 ccccTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTc                                                                         <  2:6406155/93‑1 (MQ=255)

                 ccccTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTc                                                                         >  1:5389476/1‑93 (MQ=255)

                 ccccTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTc                                                                         >  1:5059120/1‑93 (MQ=255)

                  cccTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCg                                                                        <  1:9534708/93‑1 (MQ=255)

                   ccTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGa                                                                       <  2:8597429/93‑1 (MQ=255)

                    cTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGAc                                                                      >  2:3209490/1‑93 (MQ=255)

                    cTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGAc                                                                      <  1:382245/93‑1 (MQ=255)

                    cTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGAc                                                                      >  1:839038/1‑93 (MQ=255)

                         aaaaTCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGaa                                                                 >  2:9022523/1‑93 (MQ=255)

                                 aaCGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGa                                                         >  2:5088266/1‑93 (MQ=255)

                                  aCGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGAt                                                        >  2:6367484/1‑93 (MQ=255)

                                   cGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGAtt                                                       <  2:8748407/93‑1 (MQ=255)

                                    gCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACACAGATTc                                                      >  2:5758541/1‑93 (MQ=255)

                                      tgtgGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCg                                                    >  1:1052836/1‑93 (MQ=255)

                                        tgGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTc                                                  <  2:688830/93‑1 (MQ=255)

                                        tgGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTc                                                  >  2:823192/1‑93 (MQ=255)

                                        tgGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTAGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTc                                                  >  2:4062564/1‑93 (MQ=255)

                                          gATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAg                                                >  1:4758138/1‑93 (MQ=255)

                                          gATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAg                                                >  1:2313961/1‑93 (MQ=255)

                                            ttttGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAgtg                                              >  2:1929637/1‑93 (MQ=255)

                                            ttttGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAgtg                                              <  1:2772541/93‑1 (MQ=255)

                                            ttttGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAgtg                                              >  2:8708713/1‑93 (MQ=255)

                                             tttGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAgtgt                                             <  2:6668444/93‑1 (MQ=255)

                                             tttGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAgtgt                                             <  2:682240/93‑1 (MQ=255)

                                               tGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTaa                                           >  1:2994732/1‑93 (MQ=255)

                                                    cTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAAcgcgc                                      >  1:9322290/1‑93 (MQ=255)

                                                      gACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACgcgcgc                                    >  2:1748352/1‑93 (MQ=255)

                                                       aCAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGcc                                   >  2:1218548/1‑93 (MQ=255)

                                                       aCAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGcc                                   >  1:858832/1‑93 (MQ=255)

                                                       aCAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGcc                                   <  1:7781081/93‑1 (MQ=255)

                                                        cAGTTATTCAAGACGATGCGGTTGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCa                                  <  1:7224909/93‑1 (MQ=255)

                                                        cAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCa                                  <  2:4347501/93‑1 (MQ=255)

                                                        cAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCa                                  <  2:2033312/93‑1 (MQ=255)

                                                          gTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCaga                                >  2:8604059/1‑93 (MQ=255)

                                                          gTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCaca                                >  2:2307638/1‑91 (MQ=255)

                                                           ttATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCagag                               <  2:7256870/93‑1 (MQ=255)

                                                            tATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAgg                              >  2:251179/1‑93 (MQ=255)

                                                             aTTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGc                             >  1:4304425/1‑93 (MQ=255)

                                                             aTTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGc                             <  1:3303610/93‑1 (MQ=255)

                                                              ttCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCt                            >  1:1341514/1‑93 (MQ=255)

                                                               tCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTg                           >  2:6274711/1‑93 (MQ=255)

                                                               tCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTg                           >  2:538803/1‑93 (MQ=255)

                                                                cAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGa                          <  2:6074150/93‑1 (MQ=255)

                                                                cAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGa                          >  1:3619833/1‑93 (MQ=255)

                                                                cAAGACGAAGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGa                          <  1:8768820/93‑1 (MQ=255)

                                                                 aaGACGATGCGGTCGCGTTTATAGAGGGCCGCTTCATCCCCTTTCGCCCTGAAAACATAGAGTCCGTCAGTGTAACGCGCGCCAGAGGCtaca                         >  2:3245208/1‑90 (MQ=255)

                                                                 aaGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGaa                         >  2:9635726/1‑93 (MQ=255)

                                                                 aaGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGaa                         >  1:5603956/1‑93 (MQ=255)

                                                                  aGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGaaa                        <  1:767417/93‑1 (MQ=255)

                                                                  aGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGaaa                        <  2:1265187/93‑1 (MQ=255)

                                                                    aCGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATg                      >  1:989698/1‑93 (MQ=255)

                                                                     cGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGc                     >  1:7056902/1‑93 (MQ=255)

                                                                      gATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGcc                    <  1:6433060/93‑1 (MQ=255)

                                                                      gATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGcc                    <  1:5920247/93‑1 (MQ=255)

                                                                       aTGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGccc                   >  2:9690271/1‑93 (MQ=255)

                                                                       aTGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGccc                   <  1:9861908/93‑1 (MQ=255)

                                                                        tGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCt                  >  1:4388869/1‑93 (MQ=255)

                                                                          cGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGt                >  1:8531063/1‑93 (MQ=255)

                                                                           ggTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGtt               <  2:3781489/93‑1 (MQ=255)

                                                                           ggTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGtt               <  1:6807308/93‑1 (MQ=255)

                                                                           ggTCGCGTTGATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGGAATGCCCTGtt               <  1:4450160/93‑1 (MQ=255)

                                                                            gTCGCGTTTATAGACGGTCGCTTTATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGttt              >  2:9270118/1‑93 (MQ=255)

                                                                            gTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGttt              <  2:7472036/93‑1 (MQ=255)

                                                                            gTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGttt              >  2:8449814/1‑93 (MQ=255)

                                                                              cgcgTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTga            <  2:2879103/93‑1 (MQ=255)

                                                                              cgcgTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCACTGTTTga            >  1:8193249/1‑93 (MQ=255)

                                                                               gcgTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTgag           >  1:7501435/1‑93 (MQ=255)

                                                                                cgTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTgaga          >  1:3085436/1‑93 (MQ=255)

                                                                                cgTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTgaga          >  1:5873481/1‑93 (MQ=255)

                                                                                cgTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTgaga          <  2:2710419/93‑1 (MQ=255)

                                                                                 gTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGAt         >  2:9477127/1‑93 (MQ=255)

                                                                                 gTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGAt         <  1:4102460/93‑1 (MQ=255)

                                                                                  tttATAGAGGGGTGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATg        <  2:7847324/93‑1 (MQ=255)

                                                                                  tttATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATg        >  1:1116763/1‑93 (MQ=255)

                                                                                   ttATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGc       >  1:1597984/1‑93 (MQ=255)

                                                                                      taGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCagt    <  1:6245574/93‑1 (MQ=255)

                                                                                      taGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCagt    >  1:9761584/1‑93 (MQ=255)

                                                                                      taGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCaga    >  1:3439449/1‑92 (MQ=255)

GACGCTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACAGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAG  >  NC_000913/1716059‑1716239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTGACGCTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACAGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTT  >  NC_000913/1716057‑1716241

GTGACGCTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACG                                                                                             <  SRR4302540.6590038/94‑1 (MQ=60)

GTGACGCTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACG                                                                                             >  SRR4302540.8598930/1‑94 (MQ=60)

GTGACGCTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACG                                                                                             <  SRR4302540.9906707/94‑1 (MQ=60)

 TGACGCTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGG                                                                                            >  SRR4302540.6114953/1‑94 (MQ=60)

   ACGCTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTC                                                                                          >  SRR4302540.4607260/1‑94 (MQ=60)

   ACGCTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTC                                                                                          <  SRR4302540.5856796/94‑1 (MQ=60)

    CGCTATTTTGCGCCGCCCCAGGAAAAATCTCAACGCTGTGGATTTCGTAACTGACAGTTATTCAAGACTATGCGGTCGCGTTTATAGACTGTCG                                                                                         <  SRR4302540.8335347/94‑1 (MQ=60)

    CGCTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCG                                                                                         >  SRR4302540.6406965/1‑94 (MQ=60)

    CGCTATTGTGCGCCGCACCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCG                                                                                         <  SRR4302540.8335348/94‑1 (MQ=60)

     GCTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGC                                                                                        >  SRR4302540.6001694/1‑94 (MQ=60)

     GCTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGC                                                                                        >  SRR4302540.8181207/1‑94 (MQ=60)

      CTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCT                                                                                       <  SRR4302540.1881555/94‑1 (MQ=60)

      CTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCT                                                                                       >  SRR4302540.2653518/1‑94 (MQ=60)

      CTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCT                                                                                       >  SRR4302540.2653517/1‑94 (MQ=60)

      CTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCT                                                                                       <  SRR4302540.1959552/94‑1 (MQ=60)

       TATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTT                                                                                      >  SRR4302540.9890901/1‑94 (MQ=60)

        ATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTC                                                                                     <  SRR4302540.7674240/94‑1 (MQ=60)

        ATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTC                                                                                     >  SRR4302540.726774/1‑94 (MQ=60)

        ATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTC                                                                                     <  SRR4302540.7674241/94‑1 (MQ=60)

        ATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTC                                                                                     <  SRR4302540.1156853/94‑1 (MQ=60)

         TTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCA                                                                                    <  SRR4302540.1069686/94‑1 (MQ=60)

         TTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCA                                                                                    <  SRR4302540.9609611/94‑1 (MQ=60)

          TGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCAT                                                                                   <  SRR4302540.9473027/94‑1 (MQ=60)

          TGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCAT                                                                                   <  SRR4302540.6347109/94‑1 (MQ=60)

               GCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTGAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCT                                                                              >  SRR4302540.1938950/1‑94 (MQ=60)

               GCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCT                                                                              >  SRR4302540.2545571/1‑94 (MQ=60)

               GCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCT                                                                              >  SRR4302540.8254197/1‑94 (MQ=60)

                CCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTT                                                                             >  SRR4302540.9533227/1‑94 (MQ=60)

                CCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTT                                                                             >  SRR4302540.9145555/1‑94 (MQ=60)

                 CGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTT                                                                            >  SRR4302540.6321501/1‑94 (MQ=60)

                 CGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACAGTCGCTTCATCGCCTTT                                                                            <  SRR4302540.9216406/94‑1 (MQ=60)

                 CGCCCCTGGAAAAATCTCAACGCTGTGGACTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGGTTATAGACTGTCGCTTCATCGCCTTC                                                                            >  SRR4302540.9062412/1‑94 (MQ=60)

                  GCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTC                                                                           >  SRR4302540.5448745/1‑94 (MQ=60)

                  GCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTC                                                                           >  SRR4302540.5113821/1‑94 (MQ=60)

                  GCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTC                                                                           >  SRR4302540.4921223/1‑94 (MQ=60)

                  GCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGAACAAGCGGTCGCGTTTTTATACGGTCGCTTCATCCGCTTTC                                                                           >  SRR4302540.3683757/1‑94 (MQ=60)

                   CCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCG                                                                          <  SRR4302540.8199834/94‑1 (MQ=60)

                    CCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGA                                                                         >  SRR4302540.4846486/1‑94 (MQ=60)

                    CCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGA                                                                         <  SRR4302540.9647579/94‑1 (MQ=60)

                     CCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGAC                                                                        >  SRR4302540.564835/1‑94 (MQ=60)

                     CCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGAC                                                                        >  SRR4302540.847937/1‑94 (MQ=60)

                     CCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGAC                                                                        >  SRR4302540.4401231/1‑94 (MQ=60)

                     CCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGAC                                                                        >  SRR4302540.9896289/1‑94 (MQ=60)

                     CCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGAC                                                                        >  SRR4302540.5862242/1‑94 (MQ=60)

                      CTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACC                                                                       <  SRR4302540.386198/94‑1 (MQ=60)

                       TGGAAAAATCTCAACGCTGGGGATTTTGTCACTGACAGTTATTCAAGACGATGCGGCCGCGTTTATAGACGGCCGCTTCATCGCCTTTCG‑CCAG                                                                     >  SRR4302540.3023434/1‑94 (MQ=60)

                          AAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAA                                                                   >  SRR4302540.8518683/1‑94 (MQ=60)

                           AAAATCTCAACGCTGTGGATTGTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAA                                                                  <  SRR4302540.9437476/94‑1 (MQ=60)

                            AAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAA                                                                 >  SRR4302540.955862/1‑94 (MQ=60)

                             AATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAAC                                                                >  SRR4302540.1515679/1‑94 (MQ=60)

                              ATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCGTTTCGACCAGAAAACA                                                               >  SRR4302540.8803560/1‑94 (MQ=60)

                              ATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACA                                                               <  SRR4302540.2224867/94‑1 (MQ=60)

                               tgtgacCGCTGTGGATTTTGTAACTGACACTTATTCAAGACGATGCGGTCGAGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACAT                                                              <  SRR4302540.9496420/88‑1 (MQ=60)

                                CTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATA                                                             >  SRR4302540.3496753/1‑94 (MQ=60)

                                CTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATA                                                             <  SRR4302540.195465/94‑1 (MQ=60)

                                CTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATA                                                             >  SRR4302540.4570406/1‑94 (MQ=60)

                                CTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATA                                                             >  SRR4302540.2097587/1‑94 (MQ=60)

                                CTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTCCATCGACTTTGGACCAAAAAACATA                                                             >  SRR4302540.3496751/1‑94 (MQ=60)

                                 TCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAG                                                            <  SRR4302540.7038018/94‑1 (MQ=60)

                                     CGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTC                                                        <  SRR4302540.7784832/94‑1 (MQ=60)

                                      GCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCATAAAACATAGATTCC                                                       <  SRR4302540.6794665/94‑1 (MQ=60)

                                      GCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCC                                                       <  SRR4302540.7474372/94‑1 (MQ=60)

                                      GCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCC                                                       >  SRR4302540.2789851/1‑94 (MQ=60)

                                      GCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCC                                                       >  SRR4302540.249526/1‑94 (MQ=60)

                                      GCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCC                                                       >  SRR4302540.5759362/1‑94 (MQ=60)

                                       CTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCG                                                      >  SRR4302540.1063552/1‑94 (MQ=60)

                                        ggtgggtgttgttaatgaacgtgattccAGACGATGCGGTCGCGTTTATAGACGGTCGCGTCATCGCCTTTCGACCAGAAAACATAGATTCCGT                                                     <  SRR4302540.5272110/66‑1 (MQ=60)

                                        TGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGT                                                     >  SRR4302540.9207467/1‑94 (MQ=60)

                                          TGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCA                                                   >  SRR4302540.853981/1‑94 (MQ=60)

                                          TGGATTTTGTAACTGACAGTTATTCAAGACGATGAGGTCGCGTTTATAGACCGTCGATTAATCCCCTTTTGACCAGAAAACATAGATTCCGGCA                                                   >  SRR4302540.853982/1‑94 (MQ=60)

                                           TGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAG                                                  <  SRR4302540.3192147/94‑1 (MQ=60)

                                           GGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAG                                                  >  SRR4302540.4808911/1‑94 (MQ=60)

                                           GGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAG                                                  >  SRR4302540.2338870/1‑94 (MQ=60)

                                           GGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAG                                                  >  SRR4302540.4808912/1‑94 (MQ=60)

                                             ATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTG                                                >  SRR4302540.9854318/1‑94 (MQ=60)

                                              TTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGT                                               <  SRR4302540.2802496/94‑1 (MQ=60)

                                              TTTTGTAACTGACAGTAATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCGTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGT                                               <  SRR4302540.6949223/94‑1 (MQ=60)

                                               TTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTA                                              >  SRR4302540.3925921/1‑94 (MQ=60)

                                                TTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAA                                             >  SRR4302540.3027741/1‑94 (MQ=60)

                                                TTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAA                                             >  SRR4302540.1667377/1‑94 (MQ=60)

                                                  GTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACG                                           <  SRR4302540.5967734/94‑1 (MQ=60)

                                                  GTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACG                                           <  SRR4302540.5967733/94‑1 (MQ=60)

                                                  GTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACG                                           <  SRR4302540.6714949/94‑1 (MQ=60)

                                                   TAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGC                                          >  SRR4302540.9994802/1‑94 (MQ=60)

                                                    AACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCG                                         <  SRR4302540.1531605/94‑1 (MQ=60)

                                                     ACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGC                                        >  SRR4302540.9433508/1‑94 (MQ=60)

                                                       TGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGC                                      >  SRR4302540.4063558/1‑94 (MQ=60)

                                                        GACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAACACATAGATTCCGTCAGTGTCACGCGCGCC                                     >  SRR4302540.2995292/1‑94 (MQ=60)

                                                        GACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCC                                     >  SRR4302540.867950/1‑94 (MQ=60)

                                                         ACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCA                                    >  SRR4302540.8364879/1‑94 (MQ=60)

                                                         ACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCA                                    <  SRR4302540.7872035/94‑1 (MQ=60)

                                                          CAGTTATTCAAGACGATGCGGTTGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAG                                   <  SRR4302540.7305462/94‑1 (MQ=60)

                                                          CAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAG                                   >  SRR4302540.688206/1‑94 (MQ=60)

                                                          CAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAG                                   <  SRR4302540.7305463/94‑1 (MQ=60)

                                                            GTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAG                                 <  SRR4302540.6436441/94‑1 (MQ=60)

                                                              TATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGC                               >  SRR4302540.4351278/1‑94 (MQ=60)

                                                               ATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCT                              >  SRR4302540.1355565/1‑94 (MQ=60)

                                                               ATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCT                              <  SRR4302540.3339783/94‑1 (MQ=60)

                                                                 TCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGA                            >  SRR4302540.3659435/1‑94 (MQ=60)

                                                                  CAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAA                           >  SRR4302540.5665617/1‑94 (MQ=60)

                                                                  CAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAA                           >  SRR4302540.6215576/1‑94 (MQ=60)

                                                                  CAAGACGAAGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAA                           <  SRR4302540.8870453/94‑1 (MQ=60)

                                                                   AAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAA                          >  SRR4302540.7817843/1‑94 (MQ=60)

                                                                    AGGCGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAAT                         <  SRR4302540.446005/94‑1 (MQ=60)

                                                                    AGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAAT                         <  SRR4302540.775593/94‑1 (MQ=60)

                                                                    AGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAAT                         >  SRR4302540.572805/1‑94 (MQ=60)

                                                                     GACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATG                        >  SRR4302540.999802/1‑94 (MQ=60)

                                                                      ACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGC                       >  SRR4302540.7135376/1‑94 (MQ=60)

                                                                       CGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCC                      >  SRR4302540.5355318/1‑94 (MQ=60)

                                                                        GATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCC                     <  SRR4302540.6504664/94‑1 (MQ=60)

                                                                        GATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCC                     >  SRR4302540.5144208/1‑94 (MQ=60)

                                                                        GATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCC                     <  SRR4302540.5985652/94‑1 (MQ=60)

                                                                         ATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCT                    <  SRR4302540.9977126/94‑1 (MQ=60)

                                                                         ATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCT                    >  SRR4302540.4436503/1‑94 (MQ=60)

                                                                          agccGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTG                   <  SRR4302540.3062856/90‑1 (MQ=60)

                                                                          TGCGGTCGCGTTTATAGACAGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTG                   <  SRR4302540.3381797/94‑1 (MQ=60)

                                                                           GCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGT                  >  SRR4302540.8630187/1‑94 (MQ=60)

                                                                             GGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTT                <  SRR4302540.6882633/94‑1 (MQ=60)

                                                                             GGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTT                <  SRR4302540.3145923/94‑1 (MQ=60)

                                                                             GGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTT                <  SRR4302540.4498393/94‑1 (MQ=60)

                                                                             GGTCGCGTTGATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGGAATGCCCTGTTT                <  SRR4302540.4498394/94‑1 (MQ=60)

                                                                              GTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGGGCGCCAGAGGCTGAAATGCGCTGTTTG               >  SRR4302540.8659664/1‑94 (MQ=60)

                                                                              GTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTG               >  SRR4302540.8659663/1‑94 (MQ=60)

                                                                              GTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTG               >  SRR4302540.3847256/1‑94 (MQ=60)

                                                                              GTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTG               <  SRR4302540.10012074/94‑1 (MQ=60)

                                                                               TCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCACTGTTTGA              >  SRR4302540.8288451/1‑94 (MQ=60)

                                                                                CGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAG             >  SRR4302540.4196544/1‑94 (MQ=60)

                                                                                CGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAG             >  SRR4302540.7585257/1‑94 (MQ=60)

                                                                                CGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAG             >  SRR4302540.4712219/1‑94 (MQ=60)

                                                                                 GCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGA            >  SRR4302540.5938405/1‑94 (MQ=60)

                                                                                 GCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGA            >  SRR4302540.3119289/1‑94 (MQ=60)

                                                                                  CGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGAT           <  SRR4302540.4369150/94‑1 (MQ=60)

                                                                                  CGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGAT           >  SRR4302540.3384379/1‑94 (MQ=60)

                                                                                  CGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGAT           <  SRR4302540.1002357/94‑1 (MQ=60)

                                                                                   GTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATG          <  SRR4302540.4146721/94‑1 (MQ=60)

                                                                                   GTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATG          <  SRR4302540.4146720/94‑1 (MQ=60)

                                                                                   GTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATG          >  SRR4302540.1128342/1‑94 (MQ=60)

                                                                                    TTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGC         >  SRR4302540.1615453/1‑94 (MQ=60)

                                                                                     TTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCA        >  SRR4302540.8995004/1‑94 (MQ=60)

                                                                                       ATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGT      >  SRR4302540.9876079/1‑94 (MQ=60)

                                                                                       ATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGA      >  SRR4302540.3476840/1‑94 (MQ=60)

                                                                                        TAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTA     <  SRR4302540.6315081/94‑1 (MQ=60)

                                                                                         AGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAG    >  SRR4302540.786439/1‑94 (MQ=60)

                                                                                          GACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGT   <  SRR4302540.5932275/94‑1 (MQ=60)

                                                                                          GACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGT   <  SRR4302540.1139905/94‑1 (MQ=60)

                                                                                           ACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTT  >  SRR4302540.9007854/1‑94 (MQ=60)

GTGACGCTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACAGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTT  >  NC_000913/1716057‑1716241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`