Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,723,269	G→A	P130S (CCA→TCA)	b1647 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,723,269	0	G	A	99.1%	808.5 / ‑4.3	228	P130S (CCA→TCA)	b1647	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (2/0); new base A (101/124); total (104/124)
Fisher's exact test for biased strand distribution p-value = 2.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

Only 100 of 235 total aligned reads displayed.

AGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGA  >  NC_000913/1723179‑1723358

aGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATc                                                                                         <  1:967815/93‑1 (MQ=255)

aGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATc                                                                                         <  2:245150/93‑1 (MQ=255)

  ttCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCgg                                                                                       >  1:7950207/1‑93 (MQ=255)

  ttCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCgg                                                                                       <  1:1429162/93‑1 (MQ=255)

  ttCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCgg                                                                                       <  2:8805205/93‑1 (MQ=255)

  tgcGATGCGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGCCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCgg                                                                                       <  1:1246265/91‑1 (MQ=255)

   tCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGt                                                                                      <  1:9142391/93‑1 (MQ=255)

   tCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGt                                                                                      <  1:2162209/93‑1 (MQ=255)

     gATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGttg                                                                                    >  2:3452128/1‑91 (MQ=255)

       tACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGTGGAt                                                                                  >  2:1299265/1‑93 (MQ=255)

        aCGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGAtt                                                                                 <  1:1951933/93‑1 (MQ=255)

        aCGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGAtt                                                                                 <  1:6949435/93‑1 (MQ=255)

        aCGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGAtt                                                                                 <  2:2638947/93‑1 (MQ=255)

         cGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTa                                                                                <  1:3820483/93‑1 (MQ=255)

          gCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTaa                                                                               <  1:1895871/93‑1 (MQ=255)

             aaaaTGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAg                                                                            >  2:6424702/1‑93 (MQ=255)

             aaaaTGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGAGTAACac                                                                            >  1:734303/1‑92 (MQ=255)

               aaTGACGCCCTTTGCCGCTCTGATGCAGATTTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCa                                                                          <  1:4466425/93‑1 (MQ=255)

                  gACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAAt                                                                       >  2:9302466/1‑93 (MQ=255)

                  gACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAAt                                                                       >  2:9383546/1‑93 (MQ=255)

                   aCGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATc                                                                      <  1:7551813/93‑1 (MQ=255)

                   aCGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATc                                                                      <  2:9541206/93‑1 (MQ=255)

                   aCGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATc                                                                      <  2:3901583/93‑1 (MQ=255)

                    cGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATcc                                                                     <  1:4521935/93‑1 (MQ=255)

                     gCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCa                                                                    >  1:3154254/1‑93 (MQ=255)

                      cACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAg                                                                   <  2:4882614/93‑1 (MQ=255)

                      cACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAg                                                                   >  2:5769847/1‑93 (MQ=255)

                      cACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAg                                                                   >  1:9939322/1‑93 (MQ=255)

                        cTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAgat                                                                 >  2:5310229/1‑93 (MQ=255)

                         tttGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAgatg                                                                <  1:7094444/93‑1 (MQ=255)

                          ttGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAgatga                                                               <  2:6059210/93‑1 (MQ=255)

                            gCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATc                                                             >  2:5025629/1‑93 (MQ=255)

                            gCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATc                                                             <  2:6150198/93‑1 (MQ=255)

                            gCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATc                                                             >  2:7753261/1‑93 (MQ=255)

                               gCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGt                                                          <  2:7421310/93‑1 (MQ=255)

                                ctctGATGCAGATGTTTGAACGCATCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATACACATGATCgggc                                                         >  2:5612465/1‑91 (MQ=255)

                                 tctGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTcg                                                        >  1:2735200/1‑93 (MQ=255)

                                   tGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTcgcg                                                      <  2:1634456/93‑1 (MQ=255)

                                    gATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCga                                                     <  1:6270577/93‑1 (MQ=255)

                                    gATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCga                                                     <  2:1186858/93‑1 (MQ=255)

                                     aTGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCgag                                                    >  2:9510575/1‑93 (MQ=255)

                                      tGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCgaga                                                   <  1:4292780/93‑1 (MQ=255)

                                         aGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGatta                                                >  2:5716236/1‑93 (MQ=255)

                                           aTGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGattaat                                              >  1:5588287/1‑93 (MQ=255)

                                            tGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGattaatt                                             >  1:7855403/1‑93 (MQ=255)

                                            tGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGattaatt                                             >  2:323975/1‑93 (MQ=255)

                                             gTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGattaatta                                            <  1:8811741/93‑1 (MQ=255)

                                             gTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGattaatta                                            <  1:1010707/93‑1 (MQ=255)

                                             gTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGattaatta                                            <  1:174428/93‑1 (MQ=255)

                                              tttGACCGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAg                                           <  2:9846887/93‑1 (MQ=255)

                                              tgtaaaCGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCTCGAGATTAATTAg                                           <  2:5149666/89‑1 (MQ=255)

                                               ttaaaCGCGTCCGCCACTTCATCGGCATCCATTATCGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGc                                          <  1:309875/90‑1 (MQ=255)

                                                  aaCGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGAc                                       >  1:8495471/1‑93 (MQ=255)

                                                   aCGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGCATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACt                                      <  1:5045478/93‑1 (MQ=255)

                                                    cgcgTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTg                                     <  2:2986875/93‑1 (MQ=255)

                                                      cgTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGtt                                   <  2:7526315/93‑1 (MQ=255)

                                                       gcccGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTc                                  <  2:9008296/91‑1 (MQ=255)

                                                       gTCCCCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTc                                  <  2:2662633/93‑1 (MQ=255)

                                                         ccGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCgg                                >  2:6659835/1‑93 (MQ=255)

                                                          cGCCACTTCATCGGCATCCATTTACGGGTCTGTTCGGTGGTTTAACAGCAAATCCAGATGATTGTTTGCGAGATTAATTAGCGACCGTTCGgc                               >  2:9264081/1‑93 (MQ=255)

                                                          cGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGgc                               <  1:7454159/93‑1 (MQ=255)

                                                          cGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGgc                               <  1:3657721/93‑1 (MQ=255)

                                                           gCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGGGAGATTAATTAGCGACTGTTCGgcg                              >  2:5867856/1‑93 (MQ=255)

                                                           gCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGgcg                              >  2:9512242/1‑93 (MQ=255)

                                                            ccACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGgcgc                             >  1:7676832/1‑93 (MQ=255)

                                                              ccTTTATCGGCAACCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGctc                           <  1:8184095/92‑1 (MQ=255)

                                                              aCTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGctt                           >  2:2801862/1‑92 (MQ=255)

                                                              aCTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGctc                           >  2:2747512/1‑93 (MQ=255)

                                                              aCTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGctc                           >  1:9865426/1‑93 (MQ=255)

                                                               cTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGctct                          <  1:338031/93‑1 (MQ=255)

                                                               cTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGctct                          >  1:2597342/1‑93 (MQ=255)

                                                               cTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGctct                          >  2:5692223/1‑93 (MQ=255)

                                                                 tCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTa                        <  2:1129788/93‑1 (MQ=255)

                                                                  cATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTaa                       >  1:29042/1‑93 (MQ=255)

                                                                  cATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTaa                       <  1:5364310/93‑1 (MQ=255)

                                                                  cATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTaa                       >  2:4667223/1‑93 (MQ=255)

                                                                   aTCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAat                      <  1:4689612/93‑1 (MQ=255)

                                                                   aTCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAat                      <  2:7245471/93‑1 (MQ=255)

                                                                     cGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAatga                    <  1:2846665/93‑1 (MQ=255)

                                                                     cGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAatga                    <  2:2321786/93‑1 (MQ=255)

                                                                      ggCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAatgat                   >  2:8204114/1‑93 (MQ=255)

                                                                       gCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAatgatg                  >  2:543751/1‑93 (MQ=255)

                                                                        cATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGAtgt                 >  2:7340727/1‑93 (MQ=255)

                                                                        cATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGAtgt                 <  1:4664243/93‑1 (MQ=255)

                                                                           ccATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGAt              <  2:2342701/93‑1 (MQ=255)

                                                                            cATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATc             >  2:9810475/1‑93 (MQ=255)

                                                                            cATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATc             <  2:1638338/93‑1 (MQ=255)

                                                                             aTTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATcg            >  1:9347853/1‑93 (MQ=255)

                                                                             aTTAACGGGTCTGACCGGTGGATTAACAACAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATcg            <  2:3302106/93‑1 (MQ=255)

                                                                              ttAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATcgc           <  2:2077208/93‑1 (MQ=255)

                                                                              ttAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATcgc           >  1:4987048/1‑93 (MQ=255)

                                                                               tAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATcgcg          <  2:1893503/93‑1 (MQ=255)

                                                                                 aCGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGt        <  1:659625/93‑1 (MQ=255)

                                                                                   gggTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCACGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCa      >  1:4033785/1‑93 (MQ=255)

                                                                                     gTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGt    <  1:6369427/93‑1 (MQ=255)

                                                                                      tCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTg   >  2:4276020/1‑93 (MQ=255)

                                                                                      tCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTg   >  2:798457/1‑93 (MQ=255)

                                                                                      tCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTg   >  2:6838855/1‑93 (MQ=255)

                                                                                      tCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTg   >  2:5843427/1‑93 (MQ=255)

                                                                                       cTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGa  <  1:71945/93‑1 (MQ=255)

AGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGA  >  NC_000913/1723179‑1723358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TAAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGATGTA  >  NC_000913/1723176‑1723362

TAAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGA                                                                                               >  SRR4302540.719930/1‑94 (MQ=60)

TAAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGA                                                                                               <  SRR4302540.5749582/94‑1 (MQ=60)

 AAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGAT                                                                                              >  SRR4302540.7256292/1‑94 (MQ=60)

 AAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGAT                                                                                              >  SRR4302540.1294000/1‑94 (MQ=60)

  AAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATC                                                                                             >  SRR4302540.7386400/1‑94 (MQ=60)

  AAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATC                                                                                             <  SRR4302540.9943343/94‑1 (MQ=60)

  AAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATC                                                                                             <  SRR4302540.6399470/94‑1 (MQ=60)

  AAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATC                                                                                             <  SRR4302540.1692869/94‑1 (MQ=60)

   AGTTCGATACGCCAAAATGACGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCG                                                                                            <  SRR4302540.977793/94‑1 (MQ=60)

    GTTCGATACGCCAAAATGACGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGG                                                                                           >  SRR4302540.8042884/1‑94 (MQ=60)

    GTTCGATACGCCAAAATGACGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGG                                                                                           >  SRR4302540.9703811/1‑94 (MQ=60)

    GTTCGATACGCCAAAATGACGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGG                                                                                           >  SRR4302540.6404952/1‑94 (MQ=60)

     TTCGATACGCCAAAATGACGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGT                                                                                          <  SRR4302540.1444634/94‑1 (MQ=60)

     TTCGATACGCCAAAATGACGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGT                                                                                          >  SRR4302540.9853124/1‑94 (MQ=60)

     TGCGATGCGCCAAAATGACGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGCCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGT                                                                                          <  SRR4302540.1258979/94‑1 (MQ=60)

      TCGATACGCCAAAATGACGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTG                                                                                         <  SRR4302540.4688478/94‑1 (MQ=60)

      TCGATACGCCAAAATGACGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTG                                                                                         <  SRR4302540.9252218/94‑1 (MQ=60)

      TCGATACGCCAAAATGACGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTG                                                                                         <  SRR4302540.2185502/94‑1 (MQ=60)

        GATACGCCAAAATGACGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGA                                                                                       <  SRR4302540.4411674/94‑1 (MQ=60)

         ATACGCCAAAATGACGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGAT                                                                                      >  SRR4302540.248110/1‑94 (MQ=60)

           ACGCCAAAATGACGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTA                                                                                    <  SRR4302540.7026765/94‑1 (MQ=60)

           ACGCCAAAATGACGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTA                                                                                    <  SRR4302540.1973168/94‑1 (MQ=60)

           ACGCCAAAATGACGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTA                                                                                    <  SRR4302540.2084714/94‑1 (MQ=60)

            CGCCAAAATGACGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAA                                                                                   <  SRR4302540.3862066/94‑1 (MQ=60)

            CGCCAAAATGACGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAA                                                                                   >  SRR4302540.3753426/1‑94 (MQ=60)

             GCCAAAATGACGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAAC                                                                                  <  SRR4302540.1916616/94‑1 (MQ=60)

             GCCAAAATGACGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAAC                                                                                  <  SRR4302540.3019858/94‑1 (MQ=60)

               CAAAATGACGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGAGTAACAC                                                                                >  SRR4302540.742154/1‑94 (MQ=60)

                AAAATGACGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGC                                                                               >  SRR4302540.8050354/1‑94 (MQ=60)

                 AAATGACGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCA                                                                              <  SRR4302540.9280467/94‑1 (MQ=60)

                  AATGACGCCCTTTGCCGCTCT‑‑GATGCAGATTTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAA                                                                             <  SRR4302540.4514849/94‑1 (MQ=60)

                  AATGACGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAA                                                                             >  SRR4302540.6481604/1‑94 (MQ=60)

                   ATGACGCACTCTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTACCGGGTCTGATCGGTGGATTAACAGCAAA                                                                            <  SRR4302540.3269891/94‑1 (MQ=60)

                    tgccgcattgTGCCGCTCT‑‑GATCCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAAT                                                                           <  SRR4302540.286222/84‑1 (MQ=60)

                    TGACGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAAT                                                                           <  SRR4302540.7500248/94‑1 (MQ=60)

                      ACGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCC                                                                         >  SRR4302540.4131823/1‑94 (MQ=60)

                      ACGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCC                                                                         <  SRR4302540.4564778/94‑1 (MQ=60)

                      ACGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCC                                                                         >  SRR4302540.7579498/1‑94 (MQ=60)

                      ACGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCC                                                                         <  SRR4302540.7636470/94‑1 (MQ=60)

                       CGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCA                                                                        <  SRR4302540.4570907/94‑1 (MQ=60)

                       CGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCA                                                                        >  SRR4302540.3187915/1‑94 (MQ=60)

                       CGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCA                                                                        >  SRR4302540.3188592/1‑94 (MQ=60)

                        GCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCca                                                                       >  SRR4302540.7988166/1‑92 (MQ=60)

                        GCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAG                                                                       >  SRR4302540.10055386/1‑94 (MQ=60)

                        GCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAG                                                                       >  SRR4302540.1325888/1‑94 (MQ=60)

                           CTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATG                                                                    >  SRR4302540.286413/1‑94 (MQ=60)

                           CTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATG                                                                    <  SRR4302540.5664813/94‑1 (MQ=60)

                            TTTTCCGTTCT‑‑GATGGAGATGTTTGATCGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGA                                                                   <  SRR4302540.1886511/94‑1 (MQ=60)

                            TTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGA                                                                   <  SRR4302540.7173394/94‑1 (MQ=60)

                             TTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGAT                                                                  <  SRR4302540.9122686/94‑1 (MQ=60)

                                 CGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGT                                                              >  SRR4302540.1195838/1‑94 (MQ=60)

                                 CGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGT                                                              <  SRR4302540.10034505/94‑1 (MQ=60)

                                  TCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTC                                                             <  SRR4302540.724639/94‑1 (MQ=60)

                                  GCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTC                                                             >  SRR4302540.7308301/1‑94 (MQ=60)

                                   CTCTTGGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGT                                                              <  SRR4302540.2653137/94‑1 (MQ=60)

                                   CTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCG                                                            >  SRR4302540.2764728/1‑94 (MQ=60)

                                    TCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGC                                                           >  SRR4302540.6799465/1‑94 (MQ=60)

                                         GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAG                                                        <  SRR4302540.2024286/94‑1 (MQ=60)

                                         GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAG                                                        <  SRR4302540.6340343/94‑1 (MQ=60)

                                          GTGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGA                                                       <  SRR4302540.7121068/94‑1 (MQ=60)

                                           TGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGAT                                                      <  SRR4302540.4339482/94‑1 (MQ=60)

                                            GCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATT                                                     >  SRR4302540.8910394/1‑94 (MQ=60)

                                             CAGATGTTTGAACGCGTCCGCCACTTCATAGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTGGGGAGATTA                                                    >  SRR4302540.1721889/1‑94 (MQ=60)

                                              AGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAA                                                   >  SRR4302540.603290/1‑94 (MQ=60)

                                               GATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAAT                                                  >  SRR4302540.5649792/1‑94 (MQ=60)

                                                ATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATT                                                 >  SRR4302540.7947191/1‑94 (MQ=60)

                                                  GTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAG                                               <  SRR4302540.176170/94‑1 (MQ=60)

                                                  GTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAG                                               <  SRR4302540.8913618/94‑1 (MQ=60)

                                                  GTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAG                                               <  SRR4302540.8913617/94‑1 (MQ=60)

                                                  GTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAG                                               <  SRR4302540.1020956/94‑1 (MQ=60)

                                                    TTAAACGCGTCCGCCACTTCATCGGCATCCATTATCGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCG                                             <  SRR4302540.312946/94‑1 (MQ=60)

                                                      GAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGAC                                           >  SRR4302540.8594189/1‑94 (MQ=60)

                                                        ACTCGTCCACCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTG                                         <  SRR4302540.1956401/94‑1 (MQ=60)

                                                        ACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTG                                         <  SRR4302540.690277/94‑1 (MQ=60)

                                                        ACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGCATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTG                                         <  SRR4302540.5100046/94‑1 (MQ=60)

                                                         CGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGT                                        >  SRR4302540.4717694/1‑94 (MQ=60)

                                                          GCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTT                                       <  SRR4302540.4904804/94‑1 (MQ=60)

                                                            GTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCG                                     >  SRR4302540.2657814/1‑94 (MQ=60)

                                                               CGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCG                                  <  SRR4302540.3697772/94‑1 (MQ=60)

                                                               CGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCG                                  <  SRR4302540.7537264/94‑1 (MQ=60)

                                                                GCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGC                                 >  SRR4302540.5277069/1‑94 (MQ=60)

                                                                GCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGC                                 >  SRR4302540.7763612/1‑94 (MQ=60)

                                                                  CACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTC                               >  SRR4302540.9980669/1‑94 (MQ=60)

                                                                   cctttATCGGCAACCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCT                              <  SRR4302540.8279164/89‑1 (MQ=60)

                                                                   ACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCT                              <  SRR4302540.8279165/94‑1 (MQ=60)

                                                                   ACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCT                              >  SRR4302540.2625350/1‑94 (MQ=60)

                                                                   ACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCT                              >  SRR4302540.3996822/1‑94 (MQ=60)

                                                                    CTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTT                             <  SRR4302540.341437/94‑1 (MQ=60)

                                                                      TCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAA                           >  SRR4302540.29350/1‑94 (MQ=60)

                                                                       CATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAAT                          <  SRR4302540.5077765/94‑1 (MQ=60)

                                                                       CATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAAT                          <  SRR4302540.5423238/94‑1 (MQ=60)

                                                                        ATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATG                         >  SRR4302540.3712397/1‑94 (MQ=60)

                                                                        ATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATG                         <  SRR4302540.4739765/94‑1 (MQ=60)

                                                                          CGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGAT                       <  SRR4302540.4475081/94‑1 (MQ=60)

                                                                          CGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGAT                       <  SRR4302540.2877482/94‑1 (MQ=60)

                                                                          CGGC‑TCCATTAACGGGTCTGACGGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATG                      <  SRR4302540.3547968/94‑1 (MQ=60)

                                                                           GGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATG                      <  SRR4302540.556203/94‑1 (MQ=60)

                                                                             CATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTG                    <  SRR4302540.4714174/94‑1 (MQ=60)

                                                                             CATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTG                    <  SRR4302540.4714173/94‑1 (MQ=60)

                                                                              ATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGA                   <  SRR4302540.10066419/94‑1 (MQ=60)

                                                                                CCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATC                 <  SRR4302540.7710467/94‑1 (MQ=60)

                                                                                CCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATC                 <  SRR4302540.7710468/94‑1 (MQ=60)

                                                                                CCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATC                 <  SRR4302540.3160401/94‑1 (MQ=60)

                                                                                 CATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCG                >  SRR4302540.9459204/1‑94 (MQ=60)

                                                                                  ATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGG               >  SRR4302540.8193580/1‑94 (MQ=60)

                                                                                  ATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGC               >  SRR4302540.9773794/1‑94 (MQ=60)

                                                                                  ATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGC               >  SRR4302540.5041047/1‑94 (MQ=60)

                                                                                  ATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGC               >  SRR4302540.5041046/1‑94 (MQ=60)

                                                                                      ACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTC           <  SRR4302540.666665/94‑1 (MQ=60)

                                                                                      ACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTC           <  SRR4302540.7826682/94‑1 (MQ=60)

                                                                                       CGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGTCGCTCTTAATGAGGTGATCGCGGTCG          >  SRR4302540.289621/1‑94 (MQ=60)

                                                                                       CGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCACGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCA          >  SRR4302540.4077282/1‑94 (MQ=60)

                                                                                          GTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTG       >  SRR4302540.1675805/1‑94 (MQ=60)

                                                                                          GTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTG       <  SRR4302540.6440356/94‑1 (MQ=60)

                                                                                            CTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGAT     >  SRR4302540.2054976/1‑94 (MQ=60)

                                                                                            CTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGAT     <  SRR4302540.72682/94‑1 (MQ=60)

                                                                                             TGATCTGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGATG    <  SRR4302540.2061753/94‑1 (MQ=60)

                                                                                             TGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGATG    <  SRR4302540.8415782/94‑1 (MQ=60)

                                                                                             TGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGATG    >  SRR4302540.2945853/1‑94 (MQ=60)

                                                                                             TGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGATG    >  SRR4302540.1635272/1‑94 (MQ=60)

                                                                                              GATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGATGT   >  SRR4302540.7904491/1‑94 (MQ=60)

                                                                                               ATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGATGTA  <  SRR4302540.7885725/94‑1 (MQ=60)

                                                                                               ATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGATGTA  <  SRR4302540.130108/94‑1 (MQ=60)

TAAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCT‑‑GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGATGTA  >  NC_000913/1723176‑1723362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`