Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,723,269	G→A	P130S (CCA→TCA)	b1647 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,723,269	0	G	A	100.0%	774.9 / NA	210	P130S (CCA→TCA)	b1647	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (102/108); total (102/108)

Only 100 of 217 total aligned reads displayed.

AGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGA  >  NC_000913/1723179‑1723358

aGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATc                                                                                         <  1:1380766/93‑1 (MQ=255)

aGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATc                                                                                         >  2:1756599/1‑93 (MQ=255)

 gTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCGTTAACGGGTCTGATCg                                                                                        <  2:4085434/93‑1 (MQ=255)

 gTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCg                                                                                        <  2:5356937/93‑1 (MQ=255)

  ttCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCgg                                                                                       <  2:4747950/93‑1 (MQ=255)

   tCGAGACGCCAAAATGACGCCCTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGt                                                                                      <  1:2570769/93‑1 (MQ=255)

     gATACGCCAAAATCACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTgg                                                                                    <  1:3601177/93‑1 (MQ=255)

        aCGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGAtt                                                                                 >  1:4422463/1‑93 (MQ=255)

        aCGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGAtt                                                                                 >  2:2910137/1‑93 (MQ=255)

        aCGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGAtt                                                                                 >  1:5768753/1‑93 (MQ=255)

         cGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTa                                                                                <  1:5964689/93‑1 (MQ=255)

           ccAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAAc                                                                              <  2:1936633/93‑1 (MQ=255)

            cAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACa                                                                             <  2:149521/93‑1 (MQ=255)

             aaaaTGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAg                                                                            >  1:1983930/1‑93 (MQ=255)

             aaaaTGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAg                                                                            >  2:3232301/1‑93 (MQ=255)

             aaaaTGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAg                                                                            >  2:5697261/1‑93 (MQ=255)

             aaaaTGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGGCTGATCGGTGGATTAACAg                                                                            <  2:6153220/93‑1 (MQ=255)

              aaaTGACGCACTTTGCCTCTCTGATGCAGATGTTTGAACGCGTCCGCCACGTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGc                                                                           <  1:3373142/93‑1 (MQ=255)

              aaaTGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGc                                                                           >  2:2680076/1‑93 (MQ=255)

              aaaTGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGc                                                                           >  1:3227923/1‑93 (MQ=255)

              aaaTGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGc                                                                           <  2:3129388/93‑1 (MQ=255)

               aaTGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCa                                                                          <  2:2811085/93‑1 (MQ=255)

                aTGACGCACTTTGCCGCTCTGATGCAGATTTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCaa                                                                         <  1:2203746/93‑1 (MQ=255)

                  gACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAAt                                                                       >  2:2416782/1‑93 (MQ=255)

                  gACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAAt                                                                       >  2:5583144/1‑93 (MQ=255)

                    cGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATcc                                                                     >  2:4690789/1‑93 (MQ=255)

                       ccTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAga                                                                  <  2:2590216/92‑1 (MQ=255)

                        cTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAgat                                                                 >  2:2213043/1‑93 (MQ=255)

                         tttGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAgatg                                                                >  2:624153/1‑93 (MQ=255)

                          ttGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAgatga                                                               <  1:5619735/93‑1 (MQ=255)

                          ttGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAgatga                                                               <  2:2822795/93‑1 (MQ=255)

                           tGCCTCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAgatgat                                                              <  2:723981/93‑1 (MQ=255)

                            gCCTCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATc                                                             <  2:4891395/93‑1 (MQ=255)

                            gCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATc                                                             >  2:3786213/1‑93 (MQ=255)

                             ccGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCg                                                            <  1:963061/93‑1 (MQ=255)

                             cagCTCTGATGCAGATGTTTGAAGGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCg                                                            <  1:1864871/91‑1 (MQ=255)

                              cgtgctGATGCGGATGTTTGACCGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCgg                                                           <  2:5868915/89‑1 (MQ=255)

                               gCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGt                                                          <  2:2712387/93‑1 (MQ=255)

                                gtctGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTc                                                         >  1:3980351/2‑93 (MQ=255)

                                ctctGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTc                                                         <  1:2915497/93‑1 (MQ=255)

                                ctctGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTc                                                         <  1:5532038/93‑1 (MQ=255)

                                  ctGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTcgc                                                       >  2:5066165/1‑93 (MQ=255)

                                  ctGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTcgc                                                       >  2:391821/1‑93 (MQ=255)

                                    gATGCACATGTTTGACCGCGTCCGCCACTTCATCGGCATCCATTAACGGCTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCga                                                     <  1:4651917/93‑1 (MQ=255)

                                      tGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCgaga                                                   <  1:1546541/93‑1 (MQ=255)

                                      tGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCgaga                                                   <  1:308758/93‑1 (MQ=255)

                                      tGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCgaga                                                   <  1:366604/93‑1 (MQ=255)

                                         aGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGatta                                                <  2:1809988/93‑1 (MQ=255)

                                          gATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGattaa                                               >  2:5740948/1‑93 (MQ=255)

                                            tGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGattaatt                                             <  1:470437/93‑1 (MQ=255)

                                             gTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGattaatta                                            >  2:3824574/1‑93 (MQ=255)

                                             gTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGattaatta                                            <  1:2781774/93‑1 (MQ=255)

                                             gTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGattaatta                                            <  1:494025/93‑1 (MQ=255)

                                              tttGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAg                                           >  2:4556139/1‑93 (MQ=255)

                                               ttGACCGCGTCCGCCACTTCATCGGCATCCATTAACGGTTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGc                                          <  1:1715770/93‑1 (MQ=255)

                                               ttGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGc                                          >  2:470185/1‑93 (MQ=255)

                                                tGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCg                                         <  2:1871818/93‑1 (MQ=255)

                                                  aaCGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGAc                                       <  1:2726901/93‑1 (MQ=255)

                                                   ccgcgTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACt                                      <  2:1100455/92‑1 (MQ=255)

                                                   aCGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACt                                      <  2:6016049/93‑1 (MQ=255)

                                                    cgcgTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTg                                     <  1:1176993/93‑1 (MQ=255)

                                                     gcgTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGt                                    <  2:883683/93‑1 (MQ=255)

                                                     gcgTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGt                                    <  2:1291844/93‑1 (MQ=255)

                                                       gTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTc                                  >  1:5862598/1‑93 (MQ=255)

                                                       gTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTc                                  <  1:897928/93‑1 (MQ=255)

                                                       gTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTc                                  >  1:1423819/1‑93 (MQ=255)

                                                       gTCCGCCACTTCAACGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTc                                  <  1:3750815/93‑1 (MQ=255)

                                                       gTCCGCCACTCCATCGGCATCAATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTc                                  <  2:485715/93‑1 (MQ=255)

                                                        tCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCg                                 <  1:5369354/93‑1 (MQ=255)

                                                        tCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCg                                 >  1:2029522/1‑93 (MQ=255)

                                                         ccGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCgg                                >  1:431455/1‑93 (MQ=255)

                                                            ccACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGgcgc                             >  2:5490831/1‑93 (MQ=255)

                                                            ccACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGgcgc                             >  2:3674578/1‑93 (MQ=255)

                                                            ccACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGgcgc                             >  2:2102416/1‑93 (MQ=255)

                                                              ccTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGctc                           <  2:4218795/92‑1 (MQ=255)

                                                              aCTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGctc                           <  1:5722111/93‑1 (MQ=255)

                                                                 tCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTa                        >  1:2295712/1‑93 (MQ=255)

                                                                   aTCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAat                      <  1:5052962/93‑1 (MQ=255)

                                                                    tCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAatg                     >  1:261509/1‑93 (MQ=255)

                                                                     cGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAatga                    <  2:2777734/93‑1 (MQ=255)

                                                                      ggCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAatgat                   <  1:4970882/93‑1 (MQ=255)

                                                                       gCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAatgatg                  >  1:326558/1‑93 (MQ=255)

                                                                          actaTTATCGGGTGTGATCGTGGTATTACCAGCAATCCAAGATGATCGGTCCCGAGATAAATTACCGATTGTT‑GGCGCTCTTAATGATGTGAt              <  2:772632/90‑1 (MQ=255)

                                                                           tcATTAACGGGTCTGATCGGGGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACGGTTCGGCGCTCTTAATGATGTGAt              <  1:4145071/92‑1 (MQ=255)

                                                                           ccATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGAt              >  2:4336499/1‑93 (MQ=255)

                                                                           ccATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGAt              >  1:3818876/1‑93 (MQ=255)

                                                                            cATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATc             <  1:2792951/93‑1 (MQ=255)

                                                                             aTTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATcg            >  1:4516250/1‑93 (MQ=255)

                                                                             aTTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATcg            >  2:3748671/1‑93 (MQ=255)

                                                                             aTTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATcg            >  2:1626413/1‑93 (MQ=255)

                                                                              ttAACGGGTCTGATCGGTGGATTAACAGCAAACCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATcgc           <  2:5451366/93‑1 (MQ=255)

                                                                               tAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATcgcg          <  1:126060/93‑1 (MQ=255)

                                                                                aaCGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCgg         <  2:5553912/93‑1 (MQ=255)

                                                                                aaCGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCgg         >  2:3466082/1‑93 (MQ=255)

                                                                                   gggTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCa      >  1:4053070/1‑93 (MQ=255)

                                                                                   gggTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCa      <  2:3288601/93‑1 (MQ=255)

                                                                                    ggTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAg     >  2:5939848/1‑93 (MQ=255)

                                                                                    ggTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAg     <  2:2364166/93‑1 (MQ=255)

                                                                                     gTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGt    <  1:2382352/93‑1 (MQ=255)

                                                                                       cTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGa  <  2:510659/93‑1 (MQ=255)

AGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGA  >  NC_000913/1723179‑1723358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TAAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGATGTA  >  NC_000913/1723176‑1723362

TAAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGA                                                                                               >  SRR4302533.1364025/1‑94 (MQ=60)

TAAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGA                                                                                               >  SRR4302533.2857135/1‑94 (MQ=60)

 AAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCCCTTCATCGGCATCCATTAACGGGTCTGAT                                                                                              >  SRR4302533.4611765/1‑94 (MQ=60)

 AAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGAT                                                                                              <  SRR4302533.5478083/94‑1 (MQ=60)

  AAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATC                                                                                             <  SRR4302533.3698109/94‑1 (MQ=60)

  AAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATC                                                                                             >  SRR4302533.3067852/1‑94 (MQ=60)

   AGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCG                                                                                            <  SRR4302533.1389080/94‑1 (MQ=60)

      TCGAGACGCCAAAATGACGCCCTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTG                                                                                         <  SRR4302533.2586588/94‑1 (MQ=60)

        GATACGCCAAAATCACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGA                                                                                       <  SRR4302533.3623335/94‑1 (MQ=60)

         ATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGAT                                                                                      <  SRR4302533.1647408/94‑1 (MQ=60)

          TACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATT                                                                                     >  SRR4302533.5803578/1‑94 (MQ=60)

          TACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATT                                                                                     >  SRR4302533.4449202/1‑94 (MQ=60)

           ACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTA                                                                                    <  SRR4302533.2429123/94‑1 (MQ=60)

            CGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAA                                                                                   >  SRR4302533.988711/1‑94 (MQ=60)

            CGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAA                                                                                   <  SRR4302533.6000783/94‑1 (MQ=60)

              CCAAAATGACGCACTTTGCCGTTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACA                                                                                 <  SRR4302533.2350937/94‑1 (MQ=60)

               CAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAG                                                                                >  SRR4302533.1996070/1‑94 (MQ=60)

               CAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAG                                                                                <  SRR4302533.3766136/94‑1 (MQ=60)

                AAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGC                                                                               >  SRR4302533.3247823/1‑94 (MQ=60)

                AAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGCGGATTAACAGC                                                                               >  SRR4302533.4181000/1‑94 (MQ=60)

                 AAATGACGCACTTTGCCTCTCTGATGCAGATGTTTGAACGCGTCCGCCACGTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCA                                                                              <  SRR4302533.3393973/94‑1 (MQ=60)

                  AATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAA                                                                             >  SRR4302533.2807777/1‑94 (MQ=60)

                   ATGACGCACTTTGCCGCTCTGATGCAGATTTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAA                                                                            <  SRR4302533.2217329/94‑1 (MQ=60)

                       CGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCA                                                                        >  SRR4302533.5585023/1‑94 (MQ=60)

                        GCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAG                                                                       <  SRR4302533.1258618/94‑1 (MQ=60)

                         cccttggctGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGA                                                                      <  SRR4302533.3250823/85‑1 (MQ=60)

                          CCTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGAT                                                                     <  SRR4302533.3769898/94‑1 (MQ=60)

                          ACTTTGCCGCTCTGATGCAGATGTTTGACCGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGAT                                                                     <  SRR4302533.3925708/94‑1 (MQ=60)

                          ACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGAT                                                                     >  SRR4302533.704685/1‑94 (MQ=60)

                          ACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGAT                                                                     <  SRR4302533.5659020/94‑1 (MQ=60)

                           CTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATG                                                                    >  SRR4302533.4976346/1‑94 (MQ=60)

                            TTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGA                                                                   >  SRR4302533.3624380/1‑94 (MQ=60)

                             TTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGAT                                                                  <  SRR4302533.5653700/94‑1 (MQ=60)

                                CCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGG                                                               <  SRR4302533.968719/94‑1 (MQ=60)

                                CAGCTCTGATGCAGATGTTTGAAGGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGG                                                               <  SRR4302533.1876247/94‑1 (MQ=60)

                                 CGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGT                                                              >  SRR4302533.5174809/1‑94 (MQ=60)

                                  GGTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTC                                                             >  SRR4302533.4004587/1‑94 (MQ=60)

                                  GCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTC                                                             <  SRR4302533.3787578/94‑1 (MQ=60)

                                   CTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCG                                                            <  SRR4302533.5565438/94‑1 (MQ=60)

                                   CTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCG                                                            >  SRR4302533.1849221/1‑94 (MQ=60)

                                   CTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCG                                                            <  SRR4302533.2933377/94‑1 (MQ=60)

                                    TCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGGCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGC                                                           >  SRR4302533.1822772/1‑94 (MQ=60)

                                     CTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCG                                                          >  SRR4302533.556461/1‑94 (MQ=60)

                                      TGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGG                                                         >  SRR4302533.1296890/1‑94 (MQ=60)

                                       GATGCAGATGTTTGACCGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAG                                                        <  SRR4302533.1970455/94‑1 (MQ=60)

                                       GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAG                                                        >  SRR4302533.5045274/1‑94 (MQ=60)

                                       GATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAG                                                        <  SRR4302533.2003790/94‑1 (MQ=60)

                                       GATGCACATGTTTGACCGCGTCCGCCACTTCATCGGCATCCATTAACGGCTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAG                                                        <  SRR4302533.4680105/94‑1 (MQ=60)

                                         TGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGAT                                                      <  SRR4302533.1555861/94‑1 (MQ=60)

                                         TGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGAT                                                      <  SRR4302533.310647/94‑1 (MQ=60)

                                         TGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGAT                                                      <  SRR4302533.368823/94‑1 (MQ=60)

                                          GCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATT                                                     <  SRR4302533.4711831/94‑1 (MQ=60)

                                           CAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTA                                                    <  SRR4302533.4441311/94‑1 (MQ=60)

                                           CAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTA                                                    <  SRR4302533.3606022/94‑1 (MQ=60)

                                           CAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTA                                                    <  SRR4302533.928953/94‑1 (MQ=60)

                                           CAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGGGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTA                                                    >  SRR4302533.3185903/1‑94 (MQ=60)

                                            ggatgcaTGAACGCGTCCGCCACTTCATCGGCATCAATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAA                                                   <  SRR4302533.6075496/87‑1 (MQ=60)

                                               TGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTA                                                <  SRR4302533.473249/94‑1 (MQ=60)

                                                TTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAG                                               <  SRR4302533.4819756/94‑1 (MQ=60)

                                                GTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAG                                               <  SRR4302533.2798862/94‑1 (MQ=60)

                                                GTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAG                                               <  SRR4302533.496972/94‑1 (MQ=60)

                                                GTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAG                                               <  SRR4302533.5902161/94‑1 (MQ=60)

                                                  TTGACCGCGTCCGCCACTTCATCGGCATCCATTAACGGTTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCG                                             <  SRR4302533.1726173/94‑1 (MQ=60)

                                                     AACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACT                                          <  SRR4302533.2743626/94‑1 (MQ=60)

                                                     AACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACT                                          >  SRR4302533.1430853/1‑94 (MQ=60)

                                                       CGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGT                                        <  SRR4302533.5006472/94‑1 (MQ=60)

                                                       CGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGT                                        <  SRR4302533.1184055/94‑1 (MQ=60)

                                                       CGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGT                                        <  SRR4302533.2881743/94‑1 (MQ=60)

                                                        CCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTT                                       <  SRR4302533.4031680/94‑1 (MQ=60)

                                                         CGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTC                                      >  SRR4302533.1432431/1‑94 (MQ=60)

                                                         CGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTC                                      >  SRR4302533.5898044/1‑94 (MQ=60)

                                                         CGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTC                                      <  SRR4302533.4456308/94‑1 (MQ=60)

                                                          GTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCG                                     >  SRR4302533.2042049/1‑94 (MQ=60)

                                                          GTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCG                                     >  SRR4302533.2222144/1‑94 (MQ=60)

                                                          GTCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCG                                     <  SRR4302533.903198/94‑1 (MQ=60)

                                                          GTCCGCCACTTCAACGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCG                                     <  SRR4302533.3773812/94‑1 (MQ=60)

                                                           TCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGG                                    <  SRR4302533.5401610/94‑1 (MQ=60)

                                                           TCCGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGG                                    >  SRR4302533.434027/1‑94 (MQ=60)

                                                             CGCCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGGTGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCG                                  >  SRR4302533.2752543/1‑94 (MQ=60)

                                                               CCACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCT                                >  SRR4302533.3344057/1‑94 (MQ=60)

                                                                 ACTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCT                              <  SRR4302533.5756662/94‑1 (MQ=60)

                                                                  CTTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTT                             >  SRR4302533.1453475/1‑94 (MQ=60)

                                                                   TTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTA                            >  SRR4302533.2309929/1‑94 (MQ=60)

                                                                   TTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTA                            >  SRR4302533.5786994/1‑94 (MQ=60)

                                                                   TTCATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTA                            >  SRR4302533.294114/1‑94 (MQ=60)

                                                                     CATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAAT                          >  SRR4302533.271845/1‑94 (MQ=60)

                                                                      ATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATG                         <  SRR4302533.5083512/94‑1 (MQ=60)

                                                                      ATCGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATG                         >  SRR4302533.263095/1‑94 (MQ=60)

                                                                        CGGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGAT                       <  SRR4302533.4390817/94‑1 (MQ=60)

                                                                         GGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATG                      <  SRR4302533.5001020/94‑1 (MQ=60)

                                                                         GGCATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATG                      >  SRR4302533.328544/1‑94 (MQ=60)

                                                                           CATCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTG                    >  SRR4302533.5831075/1‑94 (MQ=60)

                                                                             TCCATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGAT                  >  SRR4302533.3842230/1‑94 (MQ=60)

                                                                              TCATTAACGGGTCTGATCGGGGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACGGTTCGGCGCTCTTAATGATGTGATC                 <  SRR4302533.4170193/94‑1 (MQ=60)

                                                                               CATTACGGGGTCTGATCGGTGTATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCG                <  SRR4302533.5853845/94‑1 (MQ=60)

                                                                               CATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCG                >  SRR4302533.2621182/1‑94 (MQ=60)

                                                                               CATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCG                >  SRR4302533.4543596/1‑94 (MQ=60)

                                                                               CATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCG                <  SRR4302533.2810131/94‑1 (MQ=60)

                                                                                ATTAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGC               >  SRR4302533.215098/1‑94 (MQ=60)

                                                                                  TAACGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGG             <  SRR4302533.126849/94‑1 (MQ=60)

                                                                                     CGGGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCA          >  SRR4302533.4077752/1‑94 (MQ=60)

                                                                                       GGTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGT        >  SRR4302533.2540552/1‑94 (MQ=60)

                                                                                        GTCTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTG       <  SRR4302533.2397084/94‑1 (MQ=60)

                                                                                          CTGATCGGTGGATTACCAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGAT     <  SRR4302533.4581587/94‑1 (MQ=60)

                                                                                          CTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGAT     <  SRR4302533.4159162/94‑1 (MQ=60)

                                                                                          CTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGAT     <  SRR4302533.2281316/94‑1 (MQ=60)

                                                                                          CTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGAT     >  SRR4302533.903686/1‑94 (MQ=60)

                                                                                          CTGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGAT     >  SRR4302533.2501148/1‑94 (MQ=60)

                                                                                           TGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGATG    <  SRR4302533.5646889/94‑1 (MQ=60)

                                                                                           TGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGATG    >  SRR4302533.765582/1‑94 (MQ=60)

                                                                                           TGATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGATG    <  SRR4302533.5481590/94‑1 (MQ=60)

                                                                                            GATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGATGT   <  SRR4302533.5916294/94‑1 (MQ=60)

                                                                                             ATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGATGTA  <  SRR4302533.3234618/94‑1 (MQ=60)

                                                                                             ATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGATGTA  >  SRR4302533.4073808/1‑94 (MQ=60)

                                                                                             ATCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGATGTA  <  SRR4302533.651616/94‑1 (MQ=60)

TAAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGATGTA  >  NC_000913/1723176‑1723362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`