Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,729,644	T→C	M845T (ATG→ACG)	lhr →	Lhr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,729,644	0	T	C	99.6%	775.8 / ‑4.9	239	M845T (ATG→ACG)	lhr	Lhr
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (137/101); total (137/102)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.97e-01

Only 100 of 257 total aligned reads displayed.

CTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATG  >  NC_000913/1729554‑1729733

cTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGc                                                                                         >  2:7095943/1‑93 (MQ=255)

 tCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGAcgcg                                                                                        >  2:2945216/1‑93 (MQ=255)

  cAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGt                                                                                       <  2:6848577/93‑1 (MQ=255)

   aGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTc                                                                                      <  2:2823506/93‑1 (MQ=255)

   aGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTc                                                                                      >  2:6741898/1‑93 (MQ=255)

    gggATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGCTCGTTATGATCTTCCCGCACTGGAACGTTAGACGAGTcc                                                                                     >  1:683673/1‑92 (MQ=255)

     ggATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGc                                                                                    <  2:9276912/93‑1 (MQ=255)

     ggATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGc                                                                                    >  2:5460173/1‑93 (MQ=255)

      gATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGCTTGACGCGTCGcc                                                                                   <  1:764337/93‑1 (MQ=255)

       atatCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCCCTGGAACGTTTGACGCGTCGCCt                                                                                  >  2:6938742/1‑93 (MQ=255)

        tatCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTg                                                                                 >  1:4871697/1‑93 (MQ=255)

        tatCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTg                                                                                 >  1:3094045/1‑93 (MQ=255)

        tatCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTg                                                                                 >  1:9466785/1‑93 (MQ=255)

         atCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGa                                                                                >  2:7301464/1‑93 (MQ=255)

         atCCGGATTTTCCGGTCAGTCTCGAAACCCTACGCGAATGTATGC‑AGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGaa                                                                               <  1:3815323/93‑1 (MQ=255)

          tCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGaa                                                                               >  1:1547786/1‑93 (MQ=255)

           ccGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAAc                                                                              >  2:8183518/1‑93 (MQ=255)

             ggATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACgg                                                                            <  1:4809636/93‑1 (MQ=255)

              gATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGt                                                                           <  1:5395967/93‑1 (MQ=255)

                ttttCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTgg                                                                         >  1:1468489/1‑93 (MQ=255)

                ttttCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTGCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTgg                                                                         <  2:9846188/93‑1 (MQ=255)

                ttttCCGGTCATTCTCGAAACCCTACGAGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTgg                                                                         <  1:1872580/93‑1 (MQ=255)

                    ccGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAAAGGAGGCgca                                                                     >  2:8219242/1‑91 (MQ=255)

                        tCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTc                                                                 >  1:9255891/1‑93 (MQ=255)

                        tCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTc                                                                 >  1:856811/1‑93 (MQ=255)

                            tctcGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAat                                                             <  2:3240226/93‑1 (MQ=255)

                             ctcGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAata                                                            <  1:3018342/93‑1 (MQ=255)

                             ctcGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAata                                                            <  1:2232695/93‑1 (MQ=255)

                                 aaaCCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCg                                                        >  1:40135/1‑93 (MQ=255)

                                 aaaCCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCg                                                        >  2:84979/1‑93 (MQ=255)

                                   aCCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGAt                                                      <  2:5537696/93‑1 (MQ=255)

                                    cccTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATg                                                     <  2:7572838/93‑1 (MQ=255)

                                     ccTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGt                                                    <  1:6081616/93‑1 (MQ=255)

                                      cTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTa                                                   >  1:5814049/1‑93 (MQ=255)

                                       tACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTaa                                                  <  1:8333719/93‑1 (MQ=255)

                                        aCGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAAc                                                 >  2:5148233/1‑93 (MQ=255)

                                        aCGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAAc                                                 <  2:2340462/93‑1 (MQ=255)

                                         cgcgAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACg                                                <  2:7061735/93‑1 (MQ=255)

                                         cgcgAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACg                                                >  1:6301326/1‑93 (MQ=255)

                                          gcgAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGa                                               >  2:7668318/1‑93 (MQ=255)

                                            gAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAATGAcc                                             <  2:359283/93‑1 (MQ=255)

                                             aaTGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCa                                            >  2:7909792/1‑93 (MQ=255)

                                             aaTGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCa                                            <  2:4111913/93‑1 (MQ=255)

                                             aaTGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCa                                            <  1:4367739/93‑1 (MQ=255)

                                              aTGTGTGCAAGATGTTTATGATGTTCGCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGAccgc                                           <  1:3519642/93‑3 (MQ=255)

                                               tGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAACTTCAAATATTCGATGTAACGACCACt                                          >  2:9931390/1‑93 (MQ=255)

                                               tGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACt                                          >  1:1182683/1‑93 (MQ=255)

                                                gTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTa                                         <  1:324368/93‑1 (MQ=255)

                                                gTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTa                                         >  1:6388483/1‑93 (MQ=255)

                                                gTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTa                                         <  1:881119/93‑1 (MQ=255)

                                                 tCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTAc                                        <  1:8121790/93‑1 (MQ=255)

                                                  cTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACg                                       >  1:9191044/1‑93 (MQ=255)

                                                   tGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATc                                                          >  2:3178428/1‑73 (MQ=255)

                                                   tGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGc                                      >  1:1070173/1‑93 (MQ=255)

                                                   tGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGc                                      >  1:2129762/1‑93 (MQ=255)

                                                   tGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGc                                      <  1:1407343/93‑1 (MQ=255)

                                                   tGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGc                                      >  2:2465755/1‑93 (MQ=255)

                                                   tGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTAAAATATCCGATGTAACGACCACAACGc                                      >  1:5661479/1‑93 (MQ=255)

                                                    gCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGcc                                     >  2:1743774/1‑93 (MQ=255)

                                                    gCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGcc                                     <  1:4683986/93‑1 (MQ=255)

                                                      aaGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACCCCCt                                   >  2:2393233/1‑93 (MQ=255)

                                                       aGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTc                                  >  1:1389757/1‑93 (MQ=255)

                                                       aGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTc                                  >  2:5849664/1‑93 (MQ=255)

                                                       aGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTc                                  >  2:6441938/1‑93 (MQ=255)

                                                       aGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTc                                  <  2:4264882/93‑1 (MQ=255)

                                                       aGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTc                                  >  1:7118029/1‑93 (MQ=255)

                                                         aTGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGc                                <  1:2565757/93‑1 (MQ=255)

                                                           gTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCt                              >  2:8915147/1‑93 (MQ=255)

                                                             ttATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCttt                            <  2:4609901/93‑1 (MQ=255)

                                                             ttATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCttt                            <  2:10038795/93‑1 (MQ=255)

                                                                 gATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGcc                        >  1:6492414/1‑93 (MQ=255)

                                                                 gATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGcc                        >  2:1439993/1‑93 (MQ=255)

                                                                  aTCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCca                       <  1:3542632/93‑1 (MQ=255)

                                                                   tCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCcac                      >  1:5646904/1‑93 (MQ=255)

                                                                    ccgcccGCACAGGAACGGATGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGTCCACTACGCCCTCGCCTTTCGCcaca                     <  1:7882724/90‑1 (MQ=255)

                                                                    cTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGCTGTAAGGACCACTACTCCCTCGCCTTTCGCcaaa                     >  1:1046105/1‑91 (MQ=255)

                                                                       cccGAACTGGAACGTTTTACGCGTCGCCTGAACGGTGGCGAAATTCAAATATTCGATGTAAGGTCCACTACGCCCGCGACTTTCACCACAAGt                  >  2:7060846/1‑93 (MQ=255)

                                                                        ccGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGtt                 <  2:2129499/93‑1 (MQ=255)

                                                                          gCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTa               <  2:8012869/93‑1 (MQ=255)

                                                                          cgccTGGGACGGTTGACCGGTTGCCTGAACGGGGGGCAAAATCAAAAAACCGGAGTAACCGCCCCTTCCCCCTCGCCTTTCGCCACAAGTTTa               <  2:475318/90‑1 (MQ=255)

                                                                           cACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTAt              >  2:3818001/1‑93 (MQ=255)

                                                                            aCTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTAtt             >  1:8709619/1‑93 (MQ=255)

                                                                            aCTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTAtt             >  2:9759601/1‑93 (MQ=255)

                                                                             cTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTg            >  1:1294181/1‑93 (MQ=255)

                                                                             cTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTg            <  1:6013142/93‑1 (MQ=255)

                                                                               ggAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGtt          >  2:5383152/1‑93 (MQ=255)

                                                                               ggAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGtt          >  2:1595357/1‑93 (MQ=255)

                                                                                gAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGTCACAAGTTTATTGTTc         >  1:8986621/1‑93 (MQ=255)

                                                                                gAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTc         <  2:1827542/93‑1 (MQ=255)

                                                                                gAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTc         <  2:9422496/93‑1 (MQ=255)

                                                                                 aaCGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCg        <  1:2721394/93‑1 (MQ=255)

                                                                                 aaCGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCg        >  1:708506/1‑93 (MQ=255)

                                                                                 aaCGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCg        <  1:7723801/93‑1 (MQ=255)

                                                                                  aCGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCgg       <  2:6518777/93‑1 (MQ=255)

                                                                                   cGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGc      <  2:8052222/93‑1 (MQ=255)

                                                                                   cGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGc      <  1:9568487/93‑1 (MQ=255)

                                                                                    gTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCt     >  2:8818479/1‑93 (MQ=255)

                                                                                     tttGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTa    >  1:459459/1‑93 (MQ=255)

                                                                                     tttGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTa    <  1:4831120/93‑1 (MQ=255)

                                                                                       tGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATg  <  1:7259195/93‑1 (MQ=255)

CTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATG  >  NC_000913/1729554‑1729733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCG  >  NC_000913/1729552‑1729736

CGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACG                                                                                             >  SRR4302540.4378088/1‑94 (MQ=60)

  CTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCG                                                                                           >  SRR4302540.84278/1‑94 (MQ=60)

    CAGGGATATCCGGATTTTCCGGTCATTCTCGAACCCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTC                                                                                         <  SRR4302540.553547/94‑1 (MQ=60)

    CAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTG                                                                                         >  SRR4302540.6076752/1‑94 (MQ=60)

     AGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCG                                                                                        >  SRR4302540.691011/1‑94 (MQ=60)

     AGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCG                                                                                        >  SRR4302540.6163204/1‑94 (MQ=60)

     AGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCG                                                                                        <  SRR4302540.937079/94‑1 (MQ=60)

       GGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCC                                                                                      <  SRR4302540.9264769/94‑1 (MQ=60)

        GATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCT                                                                                     <  SRR4302540.407382/94‑1 (MQ=60)

        GATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGCTTGACGCGTCGCCT                                                                                     <  SRR4302540.772477/94‑1 (MQ=60)

         ATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGCATGTCTGCAAGATGTTTATGATCTTCACGCACTGGAACGTGTGCTGCGTCGCCGG                                                                                    >  SRR4302540.9579090/1‑94 (MQ=60)

         ATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTG                                                                                    >  SRR4302540.9579088/1‑94 (MQ=60)

         ATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTG                                                                                    >  SRR4302540.4923964/1‑94 (MQ=60)

         ATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTG                                                                                    >  SRR4302540.3127968/1‑94 (MQ=60)

         ATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTG                                                                                    >  SRR4302540.8973603/1‑94 (MQ=60)

         ATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTG                                                                                    >  SRR4302540.6123222/1‑94 (MQ=60)

           ATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAA                                                                                  >  SRR4302540.1564856/1‑94 (MQ=60)

           ATCCGGATTTTCCGGTCAGTCTCGAAACCCTACGCGAATGTATGC‑AGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAAC                                                                                 <  SRR4302540.3856867/94‑1 (MQ=60)

            TCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAAC                                                                                 <  SRR4302540.4740217/94‑1 (MQ=60)

            TCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAAC                                                                                 <  SRR4302540.3856866/94‑1 (MQ=60)

              CGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGG                                                                               >  SRR4302540.9354395/1‑94 (MQ=60)

               GGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGT                                                                              <  SRR4302540.4861115/94‑1 (MQ=60)

                GATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTG                                                                             >  SRR4302540.3409036/1‑94 (MQ=60)

                GATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTG                                                                             <  SRR4302540.3562227/94‑1 (MQ=60)

                GATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTG                                                                             <  SRR4302540.5455296/94‑1 (MQ=60)

                 ATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGG                                                                            >  SRR4302540.1484520/1‑94 (MQ=60)

                 ATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGG                                                                            <  SRR4302540.4967355/94‑1 (MQ=60)

                 ATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGGGG                                                                            >  SRR4302540.1484518/1‑94 (MQ=60)

                  TTTTCCGGTCATTCTCGAAACCCTACGAGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGC                                                                           <  SRR4302540.1893097/94‑1 (MQ=60)

                      CCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAA                                                                       >  SRR4302540.4672122/1‑94 (MQ=60)

                         GTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTC                                                                    >  SRR4302540.9366649/1‑94 (MQ=60)

                         GTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTC                                                                    >  SRR4302540.865912/1‑94 (MQ=60)

                         GTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTC                                                                    >  SRR4302540.489983/1‑94 (MQ=60)

                         GTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTA                                                                    >  SRR4302540.8839844/1‑94 (MQ=60)

                         GTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGCTTGACGCGTCGCCTGAACGGTGGCGAAATTC                                                                    >  SRR4302540.8856768/1‑94 (MQ=60)

                          TCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCA                                                                   >  SRR4302540.7168885/1‑94 (MQ=60)

                           CTTTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAA                                                                  >  SRR4302540.6426229/1‑94 (MQ=60)

                           CATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAA                                                                  >  SRR4302540.5458436/1‑94 (MQ=60)

                            ATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAA                                                                 >  SRR4302540.956091/1‑94 (MQ=60)

                               CTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATAT                                                              <  SRR4302540.2256725/94‑1 (MQ=60)

                               CTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATAT                                                              <  SRR4302540.3051519/94‑1 (MQ=60)

                                 ctcttccgatctagcgctTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCC                                                            <  SRR4302540.3212968/76‑1 (MQ=60)

                                  GAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCG                                                           >  SRR4302540.5852711/1‑94 (MQ=60)

                                  GAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCG                                                           >  SRR4302540.40547/1‑94 (MQ=60)

                                    AACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGAT                                                         <  SRR4302540.6504650/94‑1 (MQ=60)

                                      CCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGT                                                       <  SRR4302540.5832861/94‑1 (MQ=60)

                                      CCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGT                                                       >  SRR4302540.7021564/1‑94 (MQ=60)

                                      CCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGT                                                       >  SRR4302540.7830435/1‑94 (MQ=60)

                                       CCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTA                                                      >  SRR4302540.5878311/1‑94 (MQ=60)

                                       CCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTA                                                      <  SRR4302540.6149272/94‑1 (MQ=60)

                                        CTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGACATTCAAATATCCGATGTAC                                                     >  SRR4302540.8788769/1‑94 (MQ=60)

                                         TACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAAC                                                    <  SRR4302540.8430458/94‑1 (MQ=60)

                                          ACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACG                                                   >  SRR4302540.6371498/1‑94 (MQ=60)

                                          ACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACG                                                   <  SRR4302540.4154312/94‑1 (MQ=60)

                                           CGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGA                                                  >  SRR4302540.2817524/1‑94 (MQ=60)

                                            GCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGAC                                                 <  SRR4302540.3719995/94‑1 (MQ=60)

                                             CGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACC                                                <  SRR4302540.2670458/94‑1 (MQ=60)

                                               AATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCAC                                              <  SRR4302540.4415200/94‑1 (MQ=60)

                                                ATGTGTGCAAGATGTTTATGATGTTCGCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCGCT                                             <  SRR4302540.3557991/94‑1 (MQ=60)

                                                ATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT                                             >  SRR4302540.1194892/1‑94 (MQ=60)

                                                 TGTCTGCAAGATGTTTATGATCTTCCCGCCCTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAAAGACAACTA                                            >  SRR4302540.1436935/1‑94 (MQ=60)

                                                 TGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTA                                            >  SRR4302540.6459601/1‑94 (MQ=60)

                                                  GTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTAC                                           <  SRR4302540.7398518/94‑1 (MQ=60)

                                                  GTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTAC                                           <  SRR4302540.6803734/94‑1 (MQ=60)

                                                  GTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTAC                                           <  SRR4302540.327617/94‑1 (MQ=60)

                                                  GTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTAC                                           <  SRR4302540.890464/94‑1 (MQ=60)

                                                  GTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTAC                                           >  SRR4302540.332323/1‑94 (MQ=60)

                                                   TCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACG                                          <  SRR4302540.8216055/94‑1 (MQ=60)

                                                   TCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACG                                          >  SRR4302540.9301273/1‑94 (MQ=60)

                                                   TCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACG                                          <  SRR4302540.8343452/94‑1 (MQ=60)

                                                   TCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACG                                          <  SRR4302540.8343712/94‑1 (MQ=60)

                                                    CTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCGGATGTACATACCACTACGC                                         >  SRR4302540.5723819/1‑94 (MQ=60)

                                                    CTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACTC                                         >  SRR4302540.4920421/1‑94 (MQ=60)

                                                    CTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGC                                         >  SRR4302540.3867671/1‑94 (MQ=60)

                                                    CTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGC                                         >  SRR4302540.802262/1‑94 (MQ=60)

                                                    CTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGC                                         >  SRR4302540.4429344/1‑94 (MQ=60)

                                                    CTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGC                                         >  SRR4302540.1081080/1‑94 (MQ=60)

                                                    CTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGC                                         >  SRR4302540.2152613/1‑94 (MQ=60)

                                                    CTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTAAAATATCCGATGTAACGACCACAACGC                                         >  SRR4302540.5723820/1‑94 (MQ=60)

                                                     TGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCC                                        <  SRR4302540.1422496/94‑1 (MQ=60)

                                                      GCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCC                                       <  SRR4302540.4734091/94‑1 (MQ=60)

                                                       CAAGATGTTTATGATCTTCCCGCCCTGGAACGTTTGACGCGTCGGCTGCACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCT                                      <  SRR4302540.1096380/94‑1 (MQ=60)

                                                       CAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCT                                      <  SRR4302540.1096379/94‑1 (MQ=60)

                                                        AAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTC                                     >  SRR4302540.7197270/1‑94 (MQ=60)

                                                        AAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTC                                     >  SRR4302540.1404632/1‑94 (MQ=60)

                                                        AAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTC                                     >  SRR4302540.1404627/1‑94 (MQ=60)

                                                         AGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCG                                    >  SRR4302540.7877340/1‑94 (MQ=60)

                                                           ATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCC                                  >  SRR4302540.630493/1‑94 (MQ=60)

                                                           ATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCC                                  <  SRR4302540.2593416/94‑1 (MQ=60)

                                                               TTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTC                              >  SRR4302540.1615648/1‑94 (MQ=60)

                                                                  TGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCC                           >  SRR4302540.6564616/1‑94 (MQ=60)

                                                                    ATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCAC                         >  SRR4302540.5709055/1‑94 (MQ=60)

                                                                    ATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCAC                         <  SRR4302540.3581331/94‑1 (MQ=60)

                                                                     TCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGCTGTAAGGACCACTACTCCCTCGCCTTTCGCCAAA                        >  SRR4302540.1056739/1‑94 (MQ=60)

                                                                     TCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACA                        >  SRR4302540.1056744/1‑94 (MQ=60)

                                                                      CTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAG                       >  SRR4302540.6543242/1‑94 (MQ=60)

                                                                      CTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAA                       <  SRR4302540.129733/94‑1 (MQ=60)

                                                                      CTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAA                       >  SRR4302540.3109059/1‑94 (MQ=60)

                                                                       TTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAG                      <  SRR4302540.7174911/94‑1 (MQ=60)

                                                                       TTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAG                      <  SRR4302540.217213/94‑1 (MQ=60)

                                                                        TCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGTCACAAGT                     >  SRR4302540.2489223/1‑94 (MQ=60)

                                                                        TCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGT                     <  SRR4302540.1490750/94‑1 (MQ=60)

                                                                         CCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTT                    >  SRR4302540.2844611/1‑94 (MQ=60)

                                                                         CCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCCATGTAACGACCACTACGCCCTCGGCTTTCGCCACAAATT                    >  SRR4302540.9951983/1‑94 (MQ=60)

                                                                          CCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTT                   <  SRR4302540.1992331/94‑1 (MQ=60)

                                                                            GCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTAT                 <  SRR4302540.6076796/94‑1 (MQ=60)

                                                                            GCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTAT                 >  SRR4302540.4828224/1‑94 (MQ=60)

                                                                             CACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATT                <  SRR4302540.2496174/94‑1 (MQ=60)

                                                                             CACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATT                >  SRR4302540.2091279/1‑94 (MQ=60)

                                                                             CACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATT                >  SRR4302540.8810717/1‑94 (MQ=60)

                                                                              ACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTG               >  SRR4302540.9640682/1‑94 (MQ=60)

                                                                              ACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTG               >  SRR4302540.1307576/1‑94 (MQ=60)

                                                                              ACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTG               >  SRR4302540.1307577/1‑94 (MQ=60)

                                                                               CTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGT              <  SRR4302540.6079823/94‑1 (MQ=60)

                                                                                TGGAACGTTTGGCGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTT             <  SRR4302540.755684/94‑1 (MQ=60)

                                                                                TGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTT             >  SRR4302540.3211704/1‑94 (MQ=60)

                                                                                TGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTT             <  SRR4302540.1361867/94‑1 (MQ=60)

                                                                                 GGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGTCACAAGTTTATTGTTC            >  SRR4302540.9094014/1‑94 (MQ=60)

                                                                                  GAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCG           >  SRR4302540.716122/1‑94 (MQ=60)

                                                                                  GAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCG           <  SRR4302540.9292149/94‑1 (MQ=60)

                                                                                  GAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCG           <  SRR4302540.4694896/94‑1 (MQ=60)

                                                                                   AACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGG          <  SRR4302540.2750731/94‑1 (MQ=60)

                                                                                   AACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGG          <  SRR4302540.7813738/94‑1 (MQ=60)

                                                                                   AACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGG          <  SRR4302540.7813739/94‑1 (MQ=60)

                                                                                   AACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGG          <  SRR4302540.8451877/94‑1 (MQ=60)

                                                                                    ACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGACTTTCGCCACAAGTTTATTGTTCGGC         >  SRR4302540.1617274/1‑94 (MQ=60)

                                                                                     CGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCT        <  SRR4302540.7317243/94‑1 (MQ=60)

                                                                                     CGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCT        <  SRR4302540.9681552/94‑1 (MQ=60)

                                                                                      GTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTA       >  SRR4302540.464364/1‑94 (MQ=60)

                                                                                       TTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTAT      <  SRR4302540.4882869/94‑1 (MQ=60)

                                                                                       TTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCATCGCCTTTCGCCACAAGTTTATTGTTCGGCTAT      <  SRR4302540.4882868/94‑1 (MQ=60)

                                                                                         TGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGT    <  SRR4302540.7340196/94‑1 (MQ=60)

                                                                                          GACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTC   <  SRR4302540.8144571/94‑1 (MQ=60)

                                                                                          GACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTC   <  SRR4302540.7964225/94‑1 (MQ=60)

                                                                                           ACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCG  <  SRR4302540.6193484/94‑1 (MQ=60)

CGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCG  >  NC_000913/1729552‑1729736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`