Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,741,574 2 bp→GA coding (94‑95/1374 nt) ydhE → putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,741,5740AG100.0% 617.5 / NA 191S32D (AGT→GAT) ydhEputative transport protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (70/121);  total (70/121)
*NC_0009131,741,5750GA100.0% 694.0 / NA 191S32D (AGT→GAT) ydhEputative transport protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (70/121);  total (70/121)

Only 100 of 195 total aligned reads displayed.

GAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCAGTACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTG  >  NC_000913/1741486‑1741661
                                                                                         ||                                                                                      
      tatCAGTGAAGCGTGTCTGTTATTAGCATTAGCAATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGAt                                                                               <  1:1773977/93‑1 (MQ=255)
      tatCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGAt                                                                               >  2:224221/1‑93 (MQ=255)
      aatCAGTGAAGCTCGTCTGTTATTAGCATTAGCAATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATTCCGTGAt                                                                               <  2:2479208/92‑1 (MQ=255)
        tCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATgg                                                                             >  1:8110998/1‑93 (MQ=255)
        tCAGTGAAGCGCGTCTGTTATTAGCATTAGCAACCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATgg                                                                             <  1:221555/93‑1 (MQ=255)
        tCAGTGAAGCGCGGCTGTTAGTAGCATTAGTAAGCCCGGTG‑ATGCGCGCGCAAATCGCCCAAACTGCGATGGGTGTTGTCGATACCGTGATgg                                                                             <  1:7607696/93‑1 (MQ=255)
          aGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGTGTTTTGTCGATACCGTGATggcg                                                                           >  2:5541198/1‑93 (MQ=255)
           gTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATggcgg                                                                          >  1:6994326/1‑93 (MQ=255)
            tGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATggcggg                                                                         <  1:4086883/93‑1 (MQ=255)
            tGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATggcggg                                                                         <  2:6503382/93‑1 (MQ=255)
             gAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATggcgggc                                                                        <  1:8439259/93‑1 (MQ=255)
              aaGCGCGTCTGTTATTAGCATTAGCAATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATggcgggcg                                                                       >  2:2348948/1‑93 (MQ=255)
              aaGCGCGTCTGTTATTAGCATTAGCAATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATggcgggcg                                                                       <  1:5674310/93‑1 (MQ=255)
              aaGCGCGTCTGTTATTAGCATTAGCAATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATggcgggcg                                                                       >  1:2393200/1‑93 (MQ=255)
               aGCGCGTCTGTTATTAGCATTAGCAATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGgcgggcgg                                                                      >  2:1427859/1‑93 (MQ=255)
                gcgcGTCTGTTATTAGCATTAGCAATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGggcggc                                                                     >  1:5869038/1‑93 (MQ=255)
                 cgcgTCTGTTATTAGCATTAGCAATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCt                                                                    <  2:7154079/93‑1 (MQ=255)
                  gcgTCTGTTATTAGCATTAGCAATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCta                                                                   >  2:3396138/1‑93 (MQ=255)
                  gcgTCTGTTATTAGCATTAGCAATCCCGGTG‑ATGCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATGCCGTGATGGGGGGCGGCta                                                                   <  1:7919616/93‑1 (MQ=255)
                    gTCTGTTATTAGCATTAGCAATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCtata                                                                 <  2:3087201/93‑1 (MQ=255)
                    gTCTGTTATTAGCATTAGCAATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCtata                                                                 <  2:3027745/93‑1 (MQ=255)
                     tttGTTATTAGCATAACCAAT‑CCGGTGTATTCTCGCACAAACCGCCCAAACTGCGTTTGGTTTTGTCGATACCGGGAGGGCGGGCGGCTATAg                                                                <  2:2821237/91‑1 (MQ=255)
                     tCTGTTATTAGCATTAGCAATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAg                                                                <  2:3723297/93‑1 (MQ=255)
                     tCTGTTATTAGCATTAGCAATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAg                                                                >  2:8610178/1‑93 (MQ=255)
                     gcTGTTATTAGCATTAGCAATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAg                                                                <  1:4125111/92‑1 (MQ=255)
                      cTGTTATTAGCATTAGCAATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAgg                                                               >  1:268547/1‑92 (MQ=255)
                      cTGTTATTAGCATTAGCAATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGt                                                               <  2:1250105/93‑1 (MQ=255)
                       tGTTATTAGCATTAGCAATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTg                                                              >  1:10015461/1‑93 (MQ=255)
                         ttattaGCATTAGCAATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGcc                                                            >  2:1519148/1‑93 (MQ=255)
                          tattaGCATTAGCAATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCa                                                           <  2:7628395/93‑1 (MQ=255)
                           attagcattagcAATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCAc                                                          >  1:7275306/1‑93 (MQ=255)
                             tagcattagcaATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCg                                                        >  1:8177889/1‑93 (MQ=255)
                              agcattagcaATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGa                                                       <  2:418778/93‑1 (MQ=255)
                                cattagcaATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACa                                                     <  1:8999161/93‑1 (MQ=255)
                                cattagcaATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACa                                                     <  2:6238561/93‑1 (MQ=255)
                                cattagcaATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACa                                                     <  1:3109000/93‑1 (MQ=255)
                                cattagcaATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACa                                                     <  2:934397/93‑1 (MQ=255)
                                cattagcaATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAATGCCACCGACa                                                     >  2:5268119/1‑93 (MQ=255)
                                 attagcaATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACAt                                                    >  2:1486036/1‑93 (MQ=255)
                                 attagcaATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACAt                                                    >  1:1286146/1‑93 (MQ=255)
                                 attagcaATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATACTGCCACCGACAt                                                    <  1:4849627/93‑1 (MQ=255)
                                  ttagcaATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATg                                                   <  2:145794/93‑1 (MQ=255)
                                  ttagcaATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATg                                                   <  1:869237/93‑1 (MQ=255)
                                  ttagcaATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATg                                                   >  1:8360215/1‑93 (MQ=255)
                                  ttagcaATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATg                                                   >  1:6099064/1‑93 (MQ=255)
                                  ttagcaATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATg                                                   <  2:467475/93‑1 (MQ=255)
                                  ttagcaATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATg                                                   >  1:5318473/1‑93 (MQ=255)
                                   tagcaATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGAGATgg                                                  <  2:4868712/93‑1 (MQ=255)
                                    agcaATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATggc                                                 <  1:4687250/93‑1 (MQ=255)
                                    agcaATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATggc                                                 <  1:1534846/93‑1 (MQ=255)
                                    agcaATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTAATGGCGGGCGGCTATAGTGCCACCGACATggc                                                 <  2:9517045/93‑1 (MQ=255)
                                      caATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATggcgg                                               <  2:3977763/93‑1 (MQ=255)
                                        aTCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGgcggcg                                             <  1:1051305/93‑1 (MQ=255)
                                         tCCCGGTG‑ATTCTCGCGCAAATCGCCCCAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCCTTATAGCCACCCACATGGcggggg                                            >  1:9399088/1‑93 (MQ=255)
                                          cccGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGt                                           <  2:162123/93‑1 (MQ=255)
                                          cccGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGt                                           <  1:914880/93‑1 (MQ=255)
                                            cGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCCCCGACATGGcggggggcc                                         >  2:2569342/1‑90 (MQ=255)
                                             ggTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGgcgc                                        >  2:2528035/1‑93 (MQ=255)
                                              gTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGGGGTCGCt                                       >  2:9145820/1‑93 (MQ=255)
                                              gTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGGCGCt                                       >  1:6670079/1‑93 (MQ=255)
                                                  aTTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATc                                    <  1:6044330/93‑1 (MQ=255)
                                                   ttCTCGCGCAGATCGCCCAAACTACGATGGGGTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCg                                   <  2:7249164/93‑1 (MQ=255)
                                                    tctcGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCgg                                  >  2:5336142/1‑93 (MQ=255)
                                                    tctcGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCgg                                  >  1:8774295/1‑93 (MQ=255)
                                                      tcGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTa                                <  1:5514885/93‑1 (MQ=255)
                                                      tcGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTa                                <  1:6447506/93‑1 (MQ=255)
                                                       cgtgcAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTAc                               <  1:7076120/90‑1 (MQ=255)
                                                       cgcgGAAATCGCCCAACCTCCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGGGGTCGCTATCGGTAc                               <  2:4146569/93‑1 (MQ=255)
                                                       cgcgCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTAc                               <  1:4973511/93‑1 (MQ=255)
                                                       cgcgCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTAc                               <  2:9834344/93‑1 (MQ=255)
                                                       cgcgCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTAc                               <  1:5166388/93‑1 (MQ=255)
                                                        gcgcAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACt                              >  1:1595768/1‑93 (MQ=255)
                                                          gcAAGTCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTCTAGTGGCACCGACCTGGCGGCGGTCGCTATTGGGACTTc                            >  2:6493961/1‑93 (MQ=255)
                                                          gcAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTc                            <  2:6666375/93‑1 (MQ=255)
                                                            aaaTCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTa                          <  2:6231923/93‑1 (MQ=255)
                                                              aTCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCTGTACTTCTATc                        <  2:7047339/93‑1 (MQ=255)
                                                              aTCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATc                        >  1:1965693/1‑93 (MQ=255)
                                                                tgCCAAAACTGCGATGGGTTTTGGCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTg                      <  2:5130397/92‑1 (MQ=255)
                                                                cGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTg                      <  1:5282109/93‑1 (MQ=255)
                                                                  cccAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGc                    >  2:8103717/1‑93 (MQ=255)
                                                                   ccAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCt                   >  1:9302885/1‑93 (MQ=255)
                                                                   ccAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGGACTTCTATCTGGCt                   >  2:1899044/1‑93 (MQ=255)
                                                                    cAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCtt                  <  1:9254132/93‑1 (MQ=255)
                                                                      aaCTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTcc                <  1:9685917/93‑1 (MQ=255)
                                                                       aCTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCg               <  2:5703980/93‑1 (MQ=255)
                                                                       aCTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCg               <  1:1689463/93‑1 (MQ=255)
                                                                        cTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCgg              <  2:8734238/93‑1 (MQ=255)
                                                                        cTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCgg              <  2:842691/93‑1 (MQ=255)
                                                                        cTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGGCGCTATCGGTACTTCTATCTGGCTTCCgg              >  1:3196573/1‑93 (MQ=255)
                                                                         tGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGc             <  1:6702361/93‑1 (MQ=255)
                                                                         cgcgcTGGGTTTGGGCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTCCTTCTATCTGGCTTCCGGc             <  1:629063/92‑1 (MQ=255)
                                                                             aTGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATc         >  2:6378009/1‑93 (MQ=255)
                                                                               gggTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCAACGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCct       >  2:3924518/1‑93 (MQ=255)
                                                                                 gTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCctct     >  2:2315134/1‑93 (MQ=255)
                                                                                 gTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCctct     <  2:2109250/93‑1 (MQ=255)
                                                                                 gTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCctct     <  1:6569112/93‑1 (MQ=255)
                                                                                 gTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCctct     <  1:146728/93‑1 (MQ=255)
                                                                                  ttttGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCtt    <  2:3482531/93‑1 (MQ=255)
                                                                                   tttGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCttt   <  1:4551685/93‑1 (MQ=255)
                                                                                    ttGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTg  <  2:262671/93‑1 (MQ=255)
                                                                                         ||                                                                                      
GAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTG‑ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCAGTACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTG  >  NC_000913/1741486‑1741661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCAGTACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGT  >  NC_000913/1741481‑1741663
                                                                                             |                                                                                           
GTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCG                                                                                             >  SRR4302540.4485695/1‑94 (MQ=60)
    AGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATAGCCCAAACTGCGATGGGTTTTGTCAATAC                                                                                         >  SRR4302540.1492694/1‑94 (MQ=60)
     GAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACC                                                                                        <  SRR4302540.419808/94‑1 (MQ=60)
       AGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGT                                                                                      <  SRR4302540.720593/94‑1 (MQ=60)
       AGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGT                                                                                      <  SRR4302540.7218524/94‑1 (MQ=60)
        GTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTG                                                                                     <  SRR4302540.4289769/94‑1 (MQ=60)
          ATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGAT                                                                                   <  SRR4302540.2335739/94‑1 (MQ=60)
           TATCAGTGAAGCGTGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATG                                                                                  <  SRR4302540.1793239/94‑1 (MQ=60)
           TATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATG                                                                                  >  SRR4302540.8261948/1‑94 (MQ=60)
           TATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATG                                                                                  <  SRR4302540.9559255/94‑1 (MQ=60)
            ATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGG                                                                                 >  SRR4302540.8205136/1‑94 (MQ=60)
             TCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGC                                                                                <  SRR4302540.7693185/94‑1 (MQ=60)
             TCAGTGAAGCGCGTCTGTTATTAGCATTAGCAACCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGC                                                                                <  SRR4302540.223703/94‑1 (MQ=60)
             TCAGTGAAGCGCGGCTGTTAGTAGCATTAGTAAGCCCGGTGATGCGCGCGCAAATCGCCCAAACTGCGATGGGTGTTGTCGATACCGTGATGGC                                                                                <  SRR4302540.7693186/94‑1 (MQ=53)
               AGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGG                                                                              >  SRR4302540.7071955/1‑94 (MQ=60)
                GTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGG                                                                             >  SRR4302540.1433797/1‑94 (MQ=60)
                 TGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGC                                                                            <  SRR4302540.4130962/94‑1 (MQ=60)
                 TGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGC                                                                            <  SRR4302540.4130963/94‑1 (MQ=60)
                 TGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGC                                                                            <  SRR4302540.9421665/94‑1 (MQ=60)
                  GAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCG                                                                           >  SRR4302540.2418986/1‑94 (MQ=60)
                  GAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCG                                                                           <  SRR4302540.8537224/94‑1 (MQ=60)
                   AAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGG                                                                          <  SRR4302540.5736794/94‑1 (MQ=60)
                    AGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC                                                                         >  SRR4302540.5933917/1‑94 (MQ=60)
                       GCGTCTGTTATTAGCATTAGCAATCCCGGTGATGCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATGCCGTGATGGGGGGCGGC‑‑TAT                                                                    <  SRR4302540.8011923/94‑1 (MQ=60)
                        CGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATA                                                                   <  SRR4302540.8763764/94‑1 (MQ=60)
                         GTCTGTTATTAGCATTAGCAATCCCGGTGATTCTTGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAG                                                                  <  SRR4302540.1089389/94‑1 (MQ=60)
                         GTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAG                                                                  >  SRR4302540.3184730/1‑94 (MQ=60)
                          TCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGG                                                                 >  SRR4302540.271196/1‑94 (MQ=60)
                          GCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGT                                                                 <  SRR4302540.4169669/94‑1 (MQ=60)
                           CTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTG                                                                >  SRR4302540.10132049/1‑94 (MQ=60)
                             GTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCC                                                              >  SRR4302540.9164127/1‑94 (MQ=60)
                              TTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCA                                                             <  SRR4302540.6028513/94‑1 (MQ=60)
                               TATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCAC                                                            >  SRR4302540.7356484/1‑94 (MQ=60)
                                 TTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGGGGC‑‑TATAGagcaacca                                                          >  SRR4302540.3848874/1‑86 (MQ=60)
                                 TTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCG                                                          >  SRR4302540.8272881/1‑94 (MQ=60)
                                     CATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACAT                                                      <  SRR4302540.3143022/94‑1 (MQ=60)
                                     CATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACAT                                                      <  SRR4302540.9107124/94‑1 (MQ=60)
                                     CATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACAT                                                      >  SRR4302540.1299406/1‑94 (MQ=60)
                                     CATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACAT                                                      <  SRR4302540.8426082/94‑1 (MQ=60)
                                      ATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATG                                                     <  SRR4302540.4901584/94‑1 (MQ=60)
                                      ATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATG                                                     >  SRR4302540.6166963/1‑94 (MQ=60)
                                      ATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATG                                                     >  SRR4302540.8457229/1‑94 (MQ=60)
                                      ATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATG                                                     >  SRR4302540.5376712/1‑94 (MQ=60)
                                      ATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATG                                                     >  SRR4302540.6166964/1‑94 (MQ=60)
                                      ATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATACTGCCACCGACATG                                                     <  SRR4302540.4901583/94‑1 (MQ=60)
                                       TTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGG                                                    <  SRR4302540.878463/94‑1 (MQ=60)
                                         TGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCG                                                  <  SRR4302540.2995678/94‑1 (MQ=60)
                                         AGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCG                                                  <  SRR4302540.1551827/94‑1 (MQ=60)
                                         AGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCG                                                  <  SRR4302540.5630178/94‑1 (MQ=60)
                                         AGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCG                                                  <  SRR4302540.4737380/94‑1 (MQ=60)
                                         AGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCAACGACCTAGCG                                                  >  SRR4302540.6991498/1‑94 (MQ=60)
                                             ATCCCGGTGATTCTCGCGCAAATCGCCCCAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCCTTATA‑‑GCCACCCACATGGCGGGGG                                              >  SRR4302540.9510911/1‑94 (MQ=60)
                                             ATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGG                                              <  SRR4302540.1062001/94‑1 (MQ=60)
                                             ATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGG                                              <  SRR4302540.1582236/94‑1 (MQ=60)
                                               CCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTC                                            <  SRR4302540.4657506/94‑1 (MQ=60)
                                               CCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTC                                            >  SRR4302540.8813166/1‑94 (MQ=60)
                                               CCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTC                                            <  SRR4302540.924550/94‑1 (MQ=60)
                                               CCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGCCGGC‑‑TATAGTGCCACCGACGTGACGGCCGTC                                            >  SRR4302540.8813167/1‑94 (MQ=60)
                                                CCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCG                                           <  SRR4302540.5145634/94‑1 (MQ=60)
                                                 CGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGC                                          <  SRR4302540.6536657/94‑1 (MQ=60)
                                                  GGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGGCGCT                                         >  SRR4302540.6743786/1‑94 (MQ=60)
                                                     GATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGCTATC                                      <  SRR4302540.6393976/94‑1 (MQ=60)
                                                     GATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGCTATC                                      <  SRR4302540.181663/94‑1 (MQ=60)
                                                     GATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGCTATC                                      <  SRR4302540.9296641/94‑1 (MQ=60)
                                                     GATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGCTATC                                      <  SRR4302540.9407136/94‑1 (MQ=60)
                                                      ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGCTATCG                                     <  SRR4302540.6111469/94‑1 (MQ=60)
                                                       TTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGCTATCGG                                    >  SRR4302540.8875942/1‑94 (MQ=60)
                                                          TCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGGCACCGACATGGCGGCGGTCGCTATCGGGAC                                 >  SRR4302540.5814110/1‑94 (MQ=60)
                                                          TCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTAC                                 <  SRR4302540.7154398/94‑1 (MQ=60)
                                                          TCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTAC                                 <  SRR4302540.5575629/94‑1 (MQ=60)
                                                          TCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTAC                                 <  SRR4302540.6519277/94‑1 (MQ=60)
                                                          TCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTAC                                 <  SRR4302540.1653266/94‑1 (MQ=60)
                                                           CGTGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACT                                <  SRR4302540.7154860/94‑1 (MQ=60)
                                                           CGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACT                                >  SRR4302540.1613205/1‑94 (MQ=60)
                                                           CGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACT                                <  SRR4302540.9021158/94‑1 (MQ=60)
                                                           CGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACT                                <  SRR4302540.5027333/94‑1 (MQ=60)
                                                           CGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACT                                <  SRR4302540.5222579/94‑1 (MQ=60)
                                                              GCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGCTCTCGGTCGTTCT                             >  SRR4302540.5575316/1‑94 (MQ=60)
                                                               CAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTA                            <  SRR4302540.2679361/94‑1 (MQ=60)
                                                                AAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGCTCTCCGCACTTCTAT                           >  SRR4302540.1719508/1‑94 (MQ=60)
                                                                AAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTAT                           >  SRR4302540.5353858/1‑94 (MQ=60)
                                                                 AATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATC                          >  SRR4302540.1987041/1‑94 (MQ=60)
                                                                  ATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCT                         <  SRR4302540.8197715/94‑1 (MQ=60)
                                                                    CGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGG                       <  SRR4302540.5339818/94‑1 (MQ=60)
                                                                      CCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCT                     >  SRR4302540.9414000/1‑94 (MQ=60)
                                                                       CCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTT                    <  SRR4302540.1991757/94‑1 (MQ=60)
                                                                        CAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTC                   <  SRR4302540.9364883/94‑1 (MQ=60)
                                                                        CAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTC                   <  SRR4302540.6653639/94‑1 (MQ=60)
                                                                          AACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCG                 <  SRR4302540.9799864/94‑1 (MQ=60)
                                                                          AACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCG                 <  SRR4302540.7819552/94‑1 (MQ=60)
                                                                          AACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCG                 <  SRR4302540.6750430/94‑1 (MQ=60)
                                                                           ACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGG                <  SRR4302540.1707782/94‑1 (MQ=60)
                                                                           ACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGGCGCTATCGGTACTTCTATCTGGCTTCCGG                >  SRR4302540.3231292/1‑94 (MQ=60)
                                                                             TGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCG              <  SRR4302540.635755/94‑1 (MQ=60)
                                                                             TGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCG              <  SRR4302540.6776343/94‑1 (MQ=60)
                                                                                GATGGGTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATC           <  SRR4302540.4832890/94‑1 (MQ=60)
                                                                                     GTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTT      <  SRR4302540.6641902/94‑1 (MQ=60)
                                                                                     GTTTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTT      <  SRR4302540.148250/94‑1 (MQ=60)
                                                                                       TTTGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTG    <  SRR4302540.4600909/94‑1 (MQ=60)
                                                                                         TGTCGATACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGT  >  SRR4302540.1951187/1‑94 (MQ=60)
                                                                                             |                                                                                           
GTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCAGTACCGTGATGGCGGGCGGC‑‑TATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGT  >  NC_000913/1741481‑1741663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑