Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,741,574 2 bp→GA coding (94‑95/1374 nt) ydhE → putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,741,5740AG100.0% 613.4 / NA 189S32D (AGT→GAT) ydhEputative transport protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (103/86);  total (103/86)
*NC_0009131,741,5750GA100.0% 709.5 / NA 189S32D (AGT→GAT) ydhEputative transport protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (103/86);  total (103/86)

Only 100 of 192 total aligned reads displayed.

AAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCAGTACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTG  >  NC_000913/1741487‑1741661
                                                                                       ||                                                                                      
aaGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATAcc                                                                                    <  2:5851287/93‑1 (MQ=255)
  gTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGTGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGt                                                                                  <  2:5171075/93‑1 (MQ=255)
  gTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGt                                                                                  <  1:2231979/93‑1 (MQ=255)
  gTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGt                                                                                  >  1:6223642/1‑93 (MQ=255)
   tataTCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTg                                                                                 >  2:2809970/1‑93 (MQ=255)
   tataTCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTg                                                                                 >  1:2601482/1‑93 (MQ=255)
   tataTCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTg                                                                                 >  1:4065005/1‑93 (MQ=255)
     tatCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGAt                                                                               >  2:4355588/1‑93 (MQ=255)
     tatCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGAt                                                                               <  2:722255/93‑1 (MQ=255)
     tatCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGAt                                                                               <  2:1612800/93‑1 (MQ=255)
     tatCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGAt                                                                               >  2:1443330/1‑93 (MQ=255)
      atCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATg                                                                              <  1:4572621/93‑1 (MQ=255)
       tCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATgg                                                                             >  2:932641/1‑93 (MQ=255)
        cAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATggc                                                                            >  2:2526692/1‑93 (MQ=255)
         aGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATggcg                                                                           >  1:1029268/1‑93 (MQ=255)
         aGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATggcg                                                                           >  1:5078532/1‑93 (MQ=255)
          gTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATggcgg                                                                          >  1:4626299/1‑93 (MQ=255)
            gAAGCGCGTCTGTTATTAGCATTAGCAATCCGGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATggcgggc                                                                        <  1:1320727/93‑1 (MQ=255)
               gcgcGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGggcggc                                                                     <  1:5827042/93‑1 (MQ=255)
               gcgcGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGggcggc                                                                     <  1:2372928/93‑1 (MQ=255)
                 gcgTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCta                                                                   >  1:5917653/1‑93 (MQ=255)
                   gTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCtata                                                                 >  2:3314955/1‑93 (MQ=255)
                   gTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCtata                                                                 >  1:768104/1‑93 (MQ=255)
                   gTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCtata                                                                 <  2:1001127/93‑1 (MQ=255)
                   gTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCtata                                                                 <  1:3921164/93‑1 (MQ=255)
                   gTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCtata                                                                 >  1:3657886/1‑93 (MQ=255)
                    tCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAg                                                                >  1:4264765/1‑93 (MQ=255)
                      tGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTg                                                              >  2:1417902/1‑93 (MQ=255)
                      tGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTg                                                              >  2:4391868/1‑93 (MQ=255)
                       gTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGc                                                             >  1:477355/1‑93 (MQ=255)
                       gTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGc                                                             >  1:4830621/1‑93 (MQ=255)
                        ttattaGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGcc                                                            <  1:5845305/93‑1 (MQ=255)
                        ttattaGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGcc                                                            <  2:6237761/93‑1 (MQ=255)
                          attagcattagcAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCAc                                                          >  2:1183706/1‑93 (MQ=255)
                             agcattagcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGa                                                       <  1:2856256/93‑1 (MQ=255)
                             agcattagcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGa                                                       >  2:558416/1‑93 (MQ=255)
                             agcattagcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGa                                                       >  1:6301137/1‑93 (MQ=255)
                                attagcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACAt                                                    <  1:5188176/93‑1 (MQ=255)
                                attagcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACAt                                                    <  1:4114614/93‑1 (MQ=255)
                                attagcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGACGGCTATAGTGCCACCGACAt                                                    <  2:1980517/93‑1 (MQ=255)
                                 ttagcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGGCACCGACATg                                                   >  2:664563/1‑93 (MQ=255)
                                 ttagcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATg                                                   >  2:4790856/1‑93 (MQ=255)
                                  tagcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATgg                                                  <  1:4339318/93‑1 (MQ=255)
                                       aTCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGTTATAGTGCCACCGACATGgcggcg                                             >  2:4110211/1‑93 (MQ=255)
                                       aTCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGgcggcg                                             >  1:896823/1‑93 (MQ=255)
                                       aTCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGgcggcg                                             >  2:5735898/1‑93 (MQ=255)
                                       aTCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGgcggcg                                             >  2:4004617/1‑93 (MQ=255)
                                        tCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGcggcgg                                            >  1:5039573/1‑93 (MQ=255)
                                           cGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCggcgggcg                                         >  2:3038918/1‑93 (MQ=255)
                                           cGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCg                                         >  1:2449485/1‑93 (MQ=255)
                                           cGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCg                                         <  2:3063058/93‑1 (MQ=255)
                                           cGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCg                                         <  1:2264046/93‑1 (MQ=255)
                                            ggTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGc                                        <  1:1412341/93‑1 (MQ=255)
                                            ggTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGc                                        <  1:5494424/93‑1 (MQ=255)
                                             gTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCt                                       >  2:2051268/1‑93 (MQ=255)
                                             gTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCt                                       <  2:2023518/93‑1 (MQ=255)
                                             gTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGAGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCt                                       >  1:3613854/1‑93 (MQ=255)
                                              tGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCtt                                      >  2:3734744/1‑92 (MQ=255)
                                              tGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTa                                      <  1:3351096/93‑1 (MQ=255)
                                               gATTCTCGCGCAAATCGCCCAAGCTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTAt                                     <  2:1803686/93‑1 (MQ=255)
                                               gATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTAt                                     >  2:1879747/1‑93 (MQ=255)
                                               gATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTAt                                     <  1:6205212/93‑1 (MQ=255)
                                               gATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTAt                                     <  1:5669369/93‑1 (MQ=255)
                                               gATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGGCGCTAt                                     >  2:3467987/1‑93 (MQ=255)
                                                aTTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGGCGCTATc                                    >  2:5187594/1‑93 (MQ=255)
                                                   ctcGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGt                                 >  2:116237/1‑93 (MQ=255)
                                                    tcGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTa                                >  2:299958/1‑93 (MQ=255)
                                                    tcGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGGCGCTATCggga                                >  1:6024828/1‑91 (MQ=255)
                                                     cgcgCAAATCGCCCAAACTGCGATTGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTAc                               <  2:4500663/93‑1 (MQ=255)
                                                        gcAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTc                            <  1:571689/93‑1 (MQ=255)
                                                        gcAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTc                            >  2:3126994/1‑93 (MQ=255)
                                                          aaaTCGCCCAAACTGCGATTGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTa                          <  2:6047613/93‑1 (MQ=255)
                                                          aaaTCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTa                          <  2:4044235/93‑1 (MQ=255)
                                                          aaaTCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGGCGCTATCGGTACTTCTa                          >  1:5244293/1‑93 (MQ=255)
                                                           aaTCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTAt                         >  2:2697911/1‑93 (MQ=255)
                                                           aaTCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGGACTTCTAt                         >  1:4324289/1‑93 (MQ=255)
                                                            aTCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATc                        <  1:1079259/93‑1 (MQ=255)
                                                             tCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCt                       <  2:2452740/93‑1 (MQ=255)
                                                               gCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTgg                     >  2:512792/1‑93 (MQ=255)
                                                                cccAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGc                    <  2:509003/93‑1 (MQ=255)
                                                                    aaCTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTcc                >  2:222405/1‑93 (MQ=255)
                                                                    aaCTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTcc                >  1:1930871/1‑93 (MQ=255)
                                                                    aaCTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTcc                <  1:3044403/93‑1 (MQ=255)
                                                                     aCTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCg               <  1:3192725/93‑1 (MQ=255)
                                                                       tGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGc             >  2:1868750/1‑93 (MQ=255)
                                                                         cGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGa           <  2:3753935/93‑1 (MQ=255)
                                                                          gATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGAt          <  1:1042265/93‑1 (MQ=255)
                                                                          gATGGGTTTTGTCGATACCGTGATGGCGGGAGGCTATAGTGCCACCGACCTGGCGGCGGTCCCCATAGGTACTTCTATCTGGCTTCCCGCGaa          >  2:3278492/1‑92 (MQ=255)
                                                                           aTGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATc         >  2:524393/1‑93 (MQ=255)
                                                                           aTGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATc         >  2:2937245/1‑93 (MQ=255)
                                                                            tGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATcc        <  2:565929/93‑1 (MQ=255)
                                                                            tGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATcc        >  1:1214624/1‑93 (MQ=255)
                                                                             gggTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTCTCGGGACTTCTATCTGGCTTCCGGGGATCct       >  2:2489892/1‑93 (MQ=255)
                                                                             gggTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCct       >  1:2577549/1‑93 (MQ=255)
                                                                              ggTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCctc      <  1:2121209/93‑1 (MQ=255)
                                                                                ttttGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCtt    <  2:1828172/93‑1 (MQ=255)
                                                                                ttttGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCtt    >  2:831374/1‑93 (MQ=255)
                                                                                 tttGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCttt   <  1:3118650/93‑1 (MQ=255)
                                                                                  ttGTCGATACCGTGATGTCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTg  <  2:3221933/93‑1 (MQ=255)
                                                                                  ttGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTg  <  2:1370927/93‑1 (MQ=255)
                                                                                       ||                                                                                      
AAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCAGTACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTG  >  NC_000913/1741487‑1741661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCAGTACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGT  >  NC_000913/1741486‑1741663
                                                                                        |                                                                                         
GAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACC                                                                                      >  SRR4302533.5863228/1‑94 (MQ=60)
  AGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGT                                                                                    >  SRR4302533.6260939/1‑94 (MQ=60)
  AGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGT                                                                                    >  SRR4302533.4396024/1‑94 (MQ=60)
   GTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTG                                                                                   >  SRR4302533.909343/1‑94 (MQ=60)
   GTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTG                                                                                   >  SRR4302533.4089749/1‑94 (MQ=60)
   GTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTG                                                                                   >  SRR4302533.2617464/1‑94 (MQ=60)
   GTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTG                                                                                   <  SRR4302533.2245749/94‑1 (MQ=60)
      TATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATG                                                                                <  SRR4302533.831188/94‑1 (MQ=60)
      TATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATG                                                                                >  SRR4302533.3192218/1‑94 (MQ=60)
       ATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGG                                                                               >  SRR4302533.402222/1‑94 (MQ=60)
       ATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGG                                                                               <  SRR4302533.4600332/94‑1 (MQ=60)
         CAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCG                                                                             <  SRR4302533.2442191/94‑1 (MQ=60)
         CAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCG                                                                             >  SRR4302533.5109227/1‑94 (MQ=60)
         CAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCG                                                                             >  SRR4302533.1035316/1‑94 (MQ=60)
          AGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGG                                                                            >  SRR4302533.4654336/1‑94 (MQ=60)
          AGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGG                                                                            <  SRR4302533.4323390/94‑1 (MQ=60)
           GTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGG                                                                           <  SRR4302533.1796673/94‑1 (MQ=60)
            TGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGC                                                                          <  SRR4302533.200492/94‑1 (MQ=60)
             GAAGCGCGTCTGTTATTAGCATTAGCAATCCGGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCG                                                                         <  SRR4302533.1328656/94‑1 (MQ=60)
               AGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC                                                                       <  SRR4302533.2974203/94‑1 (MQ=60)
                GCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCT                                                                      <  SRR4302533.2387612/94‑1 (MQ=60)
                GCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCT                                                                      <  SRR4302533.5862251/94‑1 (MQ=60)
                 CGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTA                                                                     >  SRR4302533.5953415/1‑94 (MQ=60)
                   CGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATA                                                                   >  SRR4302533.3680378/1‑94 (MQ=60)
                   CGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATA                                                                   >  SRR4302533.772634/1‑94 (MQ=60)
                   CGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATA                                                                   <  SRR4302533.6312933/94‑1 (MQ=60)
                    GTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAG                                                                  >  SRR4302533.4290450/1‑94 (MQ=60)
                    GTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAG                                                                  <  SRR4302533.3945110/94‑1 (MQ=60)
                       TGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGC                                                               >  SRR4302533.5372919/1‑94 (MQ=60)
                       TGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGC                                                               >  SRR4302533.480205/1‑94 (MQ=60)
                       TGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGC                                                               >  SRR4302533.5999715/1‑94 (MQ=60)
                       TGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGC                                                               >  SRR4302533.4859899/1‑94 (MQ=60)
                       TGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGC                                                               >  SRR4302533.1221883/1‑94 (MQ=60)
                       TGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGGGC                                                               >  SRR4302533.1305467/1‑94 (MQ=60)
                         TTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCA                                                             <  SRR4302533.5880643/94‑1 (MQ=60)
                         TTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCA                                                             >  SRR4302533.2484946/1‑94 (MQ=60)
                         TTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCA                                                             <  SRR4302533.5090385/94‑1 (MQ=60)
                         TTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGAGCGGCTATACTGCCA                                                             >  SRR4302533.4612442/1‑94 (MQ=60)
                          TATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCAC                                                            >  SRR4302533.4150891/1‑94 (MQ=60)
                             TAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGA                                                         >  SRR4302533.6338773/1‑94 (MQ=60)
                              AGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGAC                                                        <  SRR4302533.2873831/94‑1 (MQ=60)
                                 ATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGGGGCTATAGTGCCACCGACATG                                                     >  SRR4302533.3328940/1‑94 (MQ=60)
                                 ATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATG                                                     <  SRR4302533.4139617/94‑1 (MQ=60)
                                 ATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATG                                                     <  SRR4302533.5219487/94‑1 (MQ=60)
                                  TTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGG                                                    <  SRR4302533.1064363/94‑1 (MQ=60)
                                   TAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGC                                                   <  SRR4302533.4365472/94‑1 (MQ=60)
                                   TAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGC                                                   <  SRR4302533.23112/94‑1 (MQ=60)
                                      CAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGC                                                <  SRR4302533.4778470/94‑1 (MQ=60)
                                       AATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCG                                               >  SRR4302533.6091322/1‑94 (MQ=60)
                                       AATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCG                                               >  SRR4302533.902087/1‑94 (MQ=60)
                                        ATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGG                                              >  SRR4302533.4842105/1‑94 (MQ=60)
                                        ATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGG                                              >  SRR4302533.5070083/1‑94 (MQ=60)
                                          CCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGACGGGCGGCTATAGTGCCACCGACATGGCGGCGGTC                                            <  SRR4302533.5198709/94‑1 (MQ=60)
                                           CCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCG                                           >  SRR4302533.2464603/1‑94 (MQ=60)
                                            CGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGC                                          <  SRR4302533.2278056/94‑1 (MQ=60)
                                             GGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCT                                         <  SRR4302533.5527569/94‑1 (MQ=60)
                                             GGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCT                                         <  SRR4302533.1410529/94‑1 (MQ=60)
                                             GGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCT                                         <  SRR4302533.1420880/94‑1 (MQ=60)
                                             GGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGAGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCT                                         >  SRR4302533.3636091/1‑94 (MQ=60)
                                               TGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTAT                                       <  SRR4302533.3371797/94‑1 (MQ=60)
                                                GATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATC                                      <  SRR4302533.6242426/94‑1 (MQ=60)
                                                GATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATC                                      <  SRR4302533.5703613/94‑1 (MQ=60)
                                                GATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATC                                      <  SRR4302533.76192/94‑1 (MQ=60)
                                                 ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCCCCGACATGGCGGCGGTCGCTATCG                                     <  SRR4302533.4883974/94‑1 (MQ=60)
                                                 ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGGCGCTATCG                                     >  SRR4302533.2675793/1‑94 (MQ=60)
                                                 ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATACTGCCACCGACATGGCGGCGGTCGCTATCG                                     <  SRR4302533.591443/94‑1 (MQ=60)
                                                   TCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGT                                   <  SRR4302533.3659302/94‑1 (MQ=60)
                                                    CTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGGCGCTATCGGGA                                  >  SRR4302533.6061337/1‑94 (MQ=60)
                                                     TCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTAC                                 <  SRR4302533.6252111/94‑1 (MQ=60)
                                                      CGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACT                                <  SRR4302533.3128582/94‑1 (MQ=60)
                                                         GCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCT                             <  SRR4302533.575064/94‑1 (MQ=60)
                                                          CAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTA                            >  SRR4302533.5635492/1‑94 (MQ=60)
                                                          CAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGGCGCTATCGGTACTTCTA                            >  SRR4302533.5275815/1‑94 (MQ=60)
                                                           AAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGGACTTCTAT                           >  SRR4302533.4350330/1‑94 (MQ=60)
                                                             ATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCT                         <  SRR4302533.1085620/94‑1 (MQ=60)
                                                                 CCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCT                     <  SRR4302533.3691431/94‑1 (MQ=60)
                                                                  CCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTT                    >  SRR4302533.2276979/1‑94 (MQ=60)
                                                                    AAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCC                  >  SRR4302533.1942649/1‑94 (MQ=60)
                                                                     AACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCG                 >  SRR4302533.4091088/1‑94 (MQ=60)
                                                                     AACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCG                 <  SRR4302533.3063038/94‑1 (MQ=60)
                                                                      ACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGG                >  SRR4302533.5246778/1‑94 (MQ=60)
                                                                      ACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGG                <  SRR4302533.3212364/94‑1 (MQ=60)
                                                                        TGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCG              <  SRR4302533.361876/94‑1 (MQ=60)
                                                                           GATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATC           <  SRR4302533.1048393/94‑1 (MQ=60)
                                                                            ATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCC          >  SRR4302533.1221920/1‑94 (MQ=60)
                                                                             TGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCT         >  SRR4302533.2593409/1‑94 (MQ=60)
                                                                              GGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTC        >  SRR4302533.2230430/1‑94 (MQ=60)
                                                                               GGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTTCGGCGATCCTCT       >  SRR4302533.5746745/1‑94 (MQ=60)
                                                                               GGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCT       <  SRR4302533.2134284/94‑1 (MQ=60)
                                                                                  TTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTG    <  SRR4302533.3137830/94‑1 (MQ=60)
                                                                                    TGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGT  <  SRR4302533.5902991/94‑1 (MQ=60)
                                                                                        |                                                                                         
GAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCAGTACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGT  >  NC_000913/1741486‑1741663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑