Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,751,176 G→A L227F (CTT→TTT)  b1673 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,751,1760GA91.5% 686.5 / 30.5 223L227F (CTT→TTT) b1673hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (10/9);  new base A (112/92);  total (122/101)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.29e-01

Only 100 of 230 total aligned reads displayed.

TACGTGCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCTTTC  >  NC_000913/1751086‑1751264
                                                                                          |                                                                                        
tACGTGCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAAtt                                                                                        >  1:1987094/1‑93 (MQ=255)
 aCGTGCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGCCATGGTTGTTATTCGCACCAATAAATTc                                                                                       >  2:7576115/1‑93 (MQ=255)
  cGTGCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCa                                                                                      <  2:2656826/93‑1 (MQ=255)
  cGTGCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCa                                                                                      <  2:8934423/93‑1 (MQ=255)
  cGTGCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCa                                                                                      <  2:7428778/93‑1 (MQ=255)
      cTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTCCTTGGCACGACATGGTTGTTATTCGCACAAATAAATTCAGtca                                                                                  >  2:2318500/1‑93 (MQ=255)
      cTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGtca                                                                                  <  1:6993185/93‑1 (MQ=255)
       tGTCCAGCGTGACTTGGGATCTGCATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGtcat                                                                                 <  2:7201679/93‑1 (MQ=255)
       tGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGtcat                                                                                 >  2:4921356/1‑93 (MQ=255)
       tGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGtcat                                                                                 >  2:1118808/1‑93 (MQ=255)
        gTCCAGCGTGACTTGGGATCTGAGTACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGtcatc                                                                                >  2:7049392/1‑93 (MQ=255)
        gTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGtcatc                                                                                >  1:7214142/1‑93 (MQ=255)
        gTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGtcatc                                                                                <  1:9093074/93‑1 (MQ=255)
        gTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGtcatc                                                                                <  2:4991173/93‑1 (MQ=255)
         tCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGtcatca                                                                               <  2:2062192/93‑1 (MQ=255)
         tCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGtcatca                                                                               <  1:2208529/93‑1 (MQ=255)
          ccAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTcatcat                                                                              >  2:2224428/1‑93 (MQ=255)
          ccAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTcatcat                                                                              <  1:631841/93‑1 (MQ=255)
             gCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTTAGTCATCATGTa                                                                           >  1:7967342/1‑93 (MQ=255)
              cGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTaa                                                                          <  1:1057169/93‑1 (MQ=255)
              cGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTaa                                                                          <  1:1702813/93‑1 (MQ=255)
                 gACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCa                                                                       <  2:8256090/93‑1 (MQ=255)
                   cTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCAtt                                                                     <  1:1329827/93‑1 (MQ=255)
                    ttGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTg                                                                    >  1:2716908/1‑93 (MQ=255)
                     tGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGATATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTga                                                                   <  1:6786931/93‑1 (MQ=255)
                      gggATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTgag                                                                  >  2:8355860/1‑93 (MQ=255)
                       ggATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTgaga                                                                 <  1:5131438/93‑1 (MQ=255)
                       ggATCTGAATACTCTGCCCACGACTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTgaga                                                                 <  2:5504107/93‑1 (MQ=255)
                       ggATCTGAATACTATGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGAACCAATAAGTTCAGTCATCATGTAATCATTgaga                                                                 <  1:4231420/93‑1 (MQ=255)
                        gATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGAc                                                                <  1:503277/93‑1 (MQ=255)
                          tCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGc                                                              >  2:4332939/1‑93 (MQ=255)
                          tCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGc                                                              <  2:7260359/93‑1 (MQ=255)
                            tGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCtt                                                            >  2:2979026/1‑93 (MQ=255)
                            tGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCtt                                                            >  1:7676645/1‑93 (MQ=255)
                              aaTACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCa                                                          <  2:6100179/93‑1 (MQ=255)
                              aaTACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCa                                                          <  2:9031643/93‑1 (MQ=255)
                               aTACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCAt                                                         >  2:5578106/1‑93 (MQ=255)
                                  ctctGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTTAGTCATCATGTAATCATTGAGACGCTTCAactc                                                      <  1:2913073/93‑1 (MQ=255)
                                  ctctGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCAtctc                                                      <  2:1584174/93‑1 (MQ=255)
                                  ctctGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCAtctc                                                      >  1:2563405/1‑93 (MQ=255)
                                  ctctGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGGTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCAtctc                                                      >  2:6207959/1‑93 (MQ=255)
                                   tctGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTcc                                                     <  1:7651190/93‑1 (MQ=255)
                                   tctGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTcc                                                     <  2:7157523/93‑1 (MQ=255)
                                   tctGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTcc                                                     <  2:2027197/93‑1 (MQ=255)
                                    ctGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCt                                                    <  2:9630040/93‑1 (MQ=255)
                                       cccACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGAc                                                 >  1:4783156/1‑93 (MQ=255)
                                         cACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGa                                               <  1:4626016/93‑1 (MQ=255)
                                          aCGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGAt                                              >  1:9873291/1‑93 (MQ=255)
                                            gATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCg                                            <  1:2161599/93‑1 (MQ=255)
                                            gATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCg                                            >  1:9916675/1‑93 (MQ=255)
                                            gATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCg                                            >  1:7255535/1‑93 (MQ=255)
                                             aTTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCgg                                           >  1:2848699/1‑93 (MQ=255)
                                             aTTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCgg                                           >  2:4149628/1‑93 (MQ=255)
                                               tgtgGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCa                                         <  2:1087764/93‑1 (MQ=255)
                                                 tgGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAAt                                       <  2:9516353/93‑1 (MQ=255)
                                                  ggCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATg                                      >  2:6760288/1‑93 (MQ=255)
                                                   gCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGt                                     <  2:3491199/93‑1 (MQ=255)
                                                    catACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGtt                                    <  1:535030/91‑1 (MQ=255)
                                                       aCTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGAc                                 <  2:1736240/93‑1 (MQ=255)
                                                        cTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGAcc                                <  2:6638615/93‑1 (MQ=255)
                                                         ttGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGAccc                               >  1:3395668/1‑93 (MQ=255)
                                                          tGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGAcccc                              <  1:8851277/93‑1 (MQ=255)
                                                          tGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGAcccc                              <  2:8226820/93‑1 (MQ=255)
                                                           tgCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCt                             <  1:3546683/92‑1 (MQ=255)
                                                              aCGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTc                          >  1:3843186/1‑93 (MQ=255)
                                                              aCGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTc                          >  2:3612800/1‑93 (MQ=255)
                                                              aCGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTc                          >  1:811048/1‑93 (MQ=255)
                                                              aCGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTc                          <  1:5901659/93‑1 (MQ=255)
                                                              aCGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTc                          >  1:6446685/1‑93 (MQ=255)
                                                              aCGACATGGATGATATTCGCACAAATAAATTCAGTCATCATGTAATCATAGAAAAACTTCAACTACTGACGAACGGCAATGTTGACCCCTTTc                          <  2:9244883/93‑1 (MQ=255)
                                                                gACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGt                        >  2:6741926/1‑93 (MQ=255)
                                                                  cATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTcc                      >  1:5063733/1‑93 (MQ=255)
                                                                   aTGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCTCTTTCGTccc                     >  2:615573/1‑93 (MQ=255)
                                                                    tGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCt                    >  1:3246746/1‑93 (MQ=255)
                                                                      gttgttATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTc                  <  1:9739623/93‑1 (MQ=255)
                                                                       ttgttATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCa                 >  1:458/1‑93 (MQ=255)
                                                                        tgttATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCaa                >  1:956027/1‑93 (MQ=255)
                                                                        tgttATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCaa                >  2:3152540/1‑93 (MQ=255)
                                                                         gttATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAAc               >  2:945118/1‑93 (MQ=255)
                                                                         gttATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAAc               <  2:6538382/93‑1 (MQ=255)
                                                                         gttATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAAc               <  1:5263135/93‑1 (MQ=255)
                                                                         gttATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAAc               >  1:1559507/1‑93 (MQ=255)
                                                                         gttATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAAc               >  1:5690040/1‑93 (MQ=255)
                                                                         gttATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAAc               >  2:2819322/1‑93 (MQ=255)
                                                                          ttATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAAcc              >  1:8774544/1‑93 (MQ=255)
                                                                             ttCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCa           >  2:9091742/1‑93 (MQ=255)
                                                                              tCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCaa          >  2:9291336/1‑93 (MQ=255)
                                                                                 cACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCg       >  1:7420748/1‑93 (MQ=255)
                                                                                  aCCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGc      >  2:3637198/1‑93 (MQ=255)
                                                                                  aCCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCCATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGc      >  1:6385303/1‑93 (MQ=255)
                                                                                  aCCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGc      >  2:7624886/1‑93 (MQ=255)
                                                                                   ccAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCt     >  2:3605934/1‑93 (MQ=255)
                                                                                   ccAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCt     >  2:95459/1‑93 (MQ=255)
                                                                                    cAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCtt    >  2:8629982/1‑93 (MQ=255)
                                                                                    cAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCtt    >  2:4461412/1‑93 (MQ=255)
                                                                                    cAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCtt    >  2:4692275/1‑93 (MQ=255)
                                                                                    cAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCtt    >  2:6512434/1‑93 (MQ=255)
                                                                                    cAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCtt    >  1:8196921/1‑93 (MQ=255)
                                                                                     aaTAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCttt   >  1:8680514/1‑93 (MQ=255)
                                                                                      aTAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCTTTc  >  1:3751481/1‑93 (MQ=255)
                                                                                          |                                                                                        
TACGTGCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCTTTC  >  NC_000913/1751086‑1751264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTTTACGTGCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCTTTCAGGT  >  NC_000913/1751083‑1751268
                                                                                             |                                                                                            
CTTTACGTGCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAA                                                                                              >  SRR4302540.5632357/1‑94 (MQ=60)
 TTTACGTGCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAAT                                                                                             >  SRR4302540.10006883/1‑94 (MQ=60)
 TTTACGTGCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAAT                                                                                             >  SRR4302540.8019050/1‑94 (MQ=60)
 TTTACGTGCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAAT                                                                                             <  SRR4302540.8339177/94‑1 (MQ=60)
  TTACGTGCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATT                                                                                            >  SRR4302540.2008637/1‑94 (MQ=60)
  TTACGTGCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATT                                                                                            <  SRR4302540.241097/94‑1 (MQ=60)
      GTGCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGT                                                                                        >  SRR4302540.3234545/1‑94 (MQ=60)
       ttcggtctagtgtttatttGGATCTGAATTCTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTC                                                                                       <  SRR4302540.7753983/75‑1 (MQ=60)
        GCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCA                                                                                      <  SRR4302540.9456821/94‑1 (MQ=60)
         CTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCAT                                                                                     >  SRR4302540.331722/1‑94 (MQ=60)
         CTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCAT                                                                                     >  SRR4302540.4992693/1‑94 (MQ=60)
         CTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCAT                                                                                     <  SRR4302540.7070803/94‑1 (MQ=60)
          TGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATC                                                                                    >  SRR4302540.7294557/1‑94 (MQ=60)
           GTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCA                                                                                   <  SRR4302540.9202318/94‑1 (MQ=60)
            TCCAGCGTGACTTGGGATCTGAATCCTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCAT                                                                                  <  SRR4302540.8423065/94‑1 (MQ=60)
            TCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCAT                                                                                  <  SRR4302540.2232382/94‑1 (MQ=60)
             CCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATG                                                                                 <  SRR4302540.638559/94‑1 (MQ=60)
              CAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGT                                                                                <  SRR4302540.374924/94‑1 (MQ=60)
               AGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTTAGTCATCATGTA                                                                               >  SRR4302540.8060195/1‑94 (MQ=60)
               AGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTA                                                                               <  SRR4302540.2456611/94‑1 (MQ=60)
                 CGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAAT                                                                             <  SRR4302540.3554598/94‑1 (MQ=60)
                 CGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAAT                                                                             <  SRR4302540.1067930/94‑1 (MQ=60)
                 CGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAAT                                                                             <  SRR4302540.1721241/94‑1 (MQ=60)
                      CTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTG                                                                        >  SRR4302540.2746205/1‑94 (MQ=60)
                      CTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTG                                                                        >  SRR4302540.2908745/1‑94 (MQ=60)
                      CTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTG                                                                        <  SRR4302540.1343722/94‑1 (MQ=60)
                        TGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGATATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAG                                                                      <  SRR4302540.6861980/94‑1 (MQ=60)
                        TGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAG                                                                      >  SRR4302540.5143649/1‑94 (MQ=60)
                          GGATCTGAATACTCTGCGCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGAC                                                                    <  SRR4302540.4647872/94‑1 (MQ=60)
                          GGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGAC                                                                    <  SRR4302540.5187014/94‑1 (MQ=60)
                          GGATCTGAATACTATGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGAACCAATAAGTTCAGTCATCATGTAATCATTGAGAC                                                                    <  SRR4302540.4277480/94‑1 (MQ=60)
                           GATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACG                                                                   <  SRR4302540.508670/94‑1 (MQ=60)
                              CTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTT                                                                >  SRR4302540.7763412/1‑94 (MQ=60)
                              CTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTT                                                                >  SRR4302540.7330997/1‑94 (MQ=60)
                              CTGAATACTCTGCCCACGATTGTGGCGTACTTGGAACGCCATGGTTGTTATTCGCACCATTAAATTCAGGCATCATGTAATCATTGAAACGCTT                                                                >  SRR4302540.2186452/1‑94 (MQ=60)
                               TGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTC                                                               >  SRR4302540.2859265/1‑94 (MQ=60)
                               TGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTC                                                               >  SRR4302540.6704432/1‑94 (MQ=60)
                                 AATACTCTGCCCCCGATTGTGGCGTCCTTGGCACGACATGGTTGTTATTCGCACCACTAAATTCAGTCCTCATGTAATCATTtcgcccctcact                                                             >  SRR4302540.6213908/1‑82 (MQ=60)
                                 AATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCAT                                                             >  SRR4302540.6213909/1‑94 (MQ=60)
                                 AATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCAT                                                             <  SRR4302540.4717220/94‑1 (MQ=60)
                                   TACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCT                                                           >  SRR4302540.625098/1‑94 (MQ=60)
                                   TACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCT                                                           <  SRR4302540.3834260/94‑1 (MQ=60)
                                   TACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCT                                                           <  SRR4302540.239608/94‑1 (MQ=60)
                                    ACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTC                                                          <  SRR4302540.1687145/94‑1 (MQ=60)
                                    ACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTC                                                          >  SRR4302540.2591037/1‑94 (MQ=60)
                                     CTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTTAGTCATCATGTAATCATTGAGACGCTTCAACTCC                                                         <  SRR4302540.2944930/94‑1 (MQ=60)
                                     CTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCC                                                         <  SRR4302540.5397925/94‑1 (MQ=60)
                                      TCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCT                                                        <  SRR4302540.7737322/94‑1 (MQ=60)
                                       CTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTG                                                       >  SRR4302540.95239/1‑94 (MQ=60)
                                         GCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGAC                                                     >  SRR4302540.4834287/1‑94 (MQ=60)
                                         GCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGAC                                                     >  SRR4302540.534791/1‑94 (MQ=60)
                                         GCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGAC                                                     <  SRR4302540.1109815/94‑1 (MQ=60)
                                         GCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGAC                                                     <  SRR4302540.1887890/94‑1 (MQ=60)
                                           CCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGA                                                   >  SRR4302540.644997/1‑94 (MQ=60)
                                           CCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGCCGA                                                   >  SRR4302540.7284718/1‑94 (MQ=60)
                                           CCACGATTGTGGCGTACTTGGCACGACATGGGTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGA                                                   >  SRR4302540.6870406/1‑94 (MQ=60)
                                            CACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGAT                                                  >  SRR4302540.9988597/1‑94 (MQ=60)
                                            CACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGAT                                                  <  SRR4302540.4240155/94‑1 (MQ=60)
                                            CACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGAT                                                  <  SRR4302540.4675580/94‑1 (MQ=60)
                                            CACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGAT                                                  >  SRR4302540.9988596/1‑94 (MQ=60)
                                            CACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGAT                                                  <  SRR4302540.9894964/94‑1 (MQ=60)
                                             ACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATC                                                 <  SRR4302540.486972/94‑1 (MQ=60)
                                              CGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCG                                                >  SRR4302540.7336496/1‑94 (MQ=60)
                                              CGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCG                                                >  SRR4302540.10032489/1‑94 (MQ=60)
                                               GATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGG                                               <  SRR4302540.2184881/94‑1 (MQ=60)
                                               GATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGG                                               >  SRR4302540.802009/1‑94 (MQ=60)
                                               GATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGG                                               >  SRR4302540.2879549/1‑94 (MQ=60)
                                               GATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGAACCAATAAATTCAGTCATTATGTAATCATTGCGACGCTTCATCTCCTGACGATCGG                                               >  SRR4302540.802010/1‑94 (MQ=60)
                                                GTTGTGGCGTACTTGGCAGGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGC                                              <  SRR4302540.2001469/94‑1 (MQ=60)
                                                ATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGC                                              >  SRR4302540.7030593/1‑94 (MQ=60)
                                                 TTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCA                                             <  SRR4302540.4405394/94‑1 (MQ=60)
                                                  TGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAA                                            <  SRR4302540.10018094/94‑1 (MQ=60)
                                                       CATACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTG                                       <  SRR4302540.540821/94‑1 (MQ=60)
                                                        GTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGA                                      >  SRR4302540.7757432/1‑94 (MQ=60)
                                                           CTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGTCAATGTTGACCC                                   >  SRR4302540.5490730/1‑94 (MQ=60)
                                                           CTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCC                                   >  SRR4302540.853952/1‑94 (MQ=60)
                                                           CTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCC                                   >  SRR4302540.3432708/1‑94 (MQ=60)
                                                           CTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCC                                   >  SRR4302540.7355793/1‑94 (MQ=60)
                                                            TTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCC                                  >  SRR4302540.10066445/1‑94 (MQ=60)
                                                             TGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCT                                 <  SRR4302540.8953671/94‑1 (MQ=60)
                                                              TGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTT                                <  SRR4302540.6635221/94‑1 (MQ=60)
                                                              TGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTT                                <  SRR4302540.3585422/94‑1 (MQ=60)
                                                                GACGACATGGTTTTTAATCCCACCAATAAATTCAGTCATCAAGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTC                              <  SRR4302540.8105413/94‑1 (MQ=60)
                                                                CACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTC                              >  SRR4302540.3884982/1‑94 (MQ=60)
                                                                CACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTC                              <  SRR4302540.9173156/94‑1 (MQ=60)
                                                                CACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTC                              >  SRR4302540.819663/1‑94 (MQ=60)
                                                                CACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTC                              >  SRR4302540.819662/1‑94 (MQ=60)
                                                                CACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTC                              >  SRR4302540.7404264/1‑94 (MQ=60)
                                                                CACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTC                              >  SRR4302540.6518448/1‑94 (MQ=60)
                                                                 ACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCG                             <  SRR4302540.5966831/94‑1 (MQ=60)
                                                                    ACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCC                          >  SRR4302540.5118481/1‑94 (MQ=60)
                                                                     CATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGCCCC                         <  SRR4302540.1477603/94‑1 (MQ=60)
                                                                      ATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCACT                        >  SRR4302540.2725673/1‑94 (MQ=60)
                                                                      ATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCT                        >  SRR4302540.9761290/1‑94 (MQ=60)
                                                                      ATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCT                        >  SRR4302540.5108490/1‑94 (MQ=60)
                                                                      ATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCT                        <  SRR4302540.890922/94‑1 (MQ=60)
                                                                      ATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCT                        >  SRR4302540.3282101/1‑94 (MQ=60)
                                                                      ATGGGTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCT                        <  SRR4302540.2891197/94‑1 (MQ=60)
                                                                        GGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTC                      <  SRR4302540.76109/94‑1 (MQ=60)
                                                                         GTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCA                     >  SRR4302540.780536/1‑94 (MQ=60)
                                                                         GTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCA                     >  SRR4302540.462/1‑94 (MQ=60)
                                                                         GTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCA                     <  SRR4302540.9853959/94‑1 (MQ=60)
                                                                          TTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAA                    >  SRR4302540.965937/1‑94 (MQ=60)
                                                                           TGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAAC                   >  SRR4302540.1576638/1‑94 (MQ=60)
                                                                           TGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAAC                   >  SRR4302540.5752678/1‑94 (MQ=60)
                                                                           TGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAAC                   >  SRR4302540.5752963/1‑94 (MQ=60)
                                                                            GTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACC                  >  SRR4302540.4511898/1‑94 (MQ=60)
                                                                            GTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACC                  >  SRR4302540.8876192/1‑94 (MQ=60)
                                                                            GTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACC                  <  SRR4302540.5320563/94‑1 (MQ=60)
                                                                              TATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGC                >  SRR4302540.8814206/1‑94 (MQ=60)
                                                                              TATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGC                >  SRR4302540.5238924/1‑94 (MQ=60)
                                                                                  CGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATC            <  SRR4302540.3632034/94‑1 (MQ=60)
                                                                                   GCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCG           <  SRR4302540.2143918/94‑1 (MQ=60)
                                                                                   GCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCG           >  SRR4302540.7503401/1‑94 (MQ=60)
                                                                                    CACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCCATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGC          >  SRR4302540.6456385/1‑94 (MQ=60)
                                                                                    CACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGC          >  SRR4302540.6456386/1‑94 (MQ=60)
                                                                                      CCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCTT        >  SRR4302540.8292180/1‑94 (MQ=60)
                                                                                       CAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCTTT       >  SRR4302540.8781326/1‑94 (MQ=60)
                                                                                        AATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCTTTC      >  SRR4302540.3792408/1‑94 (MQ=60)
                                                                                          TAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCTTTCAG    >  SRR4302540.1416348/1‑94 (MQ=60)
                                                                                            AATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCTTTCAGGT  >  SRR4302540.5364559/1‑94 (MQ=60)
                                                                                             |                                                                                            
CTTTACGTGCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCTTTCAGGT  >  NC_000913/1751083‑1751268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑