Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,751,176 G→A L227F (CTT→TTT)  b1673 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,751,1760GA99.5% 758.7 / ‑5.4 204L227F (CTT→TTT) b1673hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (109/94);  total (110/94)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.35e-01

Only 100 of 208 total aligned reads displayed.

TACGTGCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCTTTCA  >  NC_000913/1751086‑1751265
                                                                                          |                                                                                         
tACGTGCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAAtt                                                                                         >  2:3929894/1‑93 (MQ=255)
tACGTGCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAAtt                                                                                         >  2:5065783/1‑93 (MQ=255)
tACGTGCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAAtt                                                                                         >  1:300619/1‑93 (MQ=255)
 aCGTGCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTc                                                                                        <  2:2657778/93‑1 (MQ=255)
 aCGTGCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTc                                                                                        >  1:3884378/1‑93 (MQ=255)
   gTGCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAg                                                                                      <  1:5352426/93‑1 (MQ=255)
   gTGCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAg                                                                                      >  1:3310406/1‑93 (MQ=255)
     gCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGTCATGGTTGTTATTCGCACCAATAAATTCAGtc                                                                                    >  1:675868/1‑93 (MQ=255)
     gCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGtc                                                                                    <  1:1201753/93‑1 (MQ=255)
     gCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGtc                                                                                    <  2:238108/93‑1 (MQ=255)
        gTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGtcatc                                                                                 <  1:5145847/93‑1 (MQ=255)
        gTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGtcatc                                                                                 <  2:5902290/93‑1 (MQ=255)
            aGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGt                                                                             >  1:4893189/1‑93 (MQ=255)
             gCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTa                                                                            <  2:4179484/93‑1 (MQ=255)
             gCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTa                                                                            >  2:5349193/1‑93 (MQ=255)
             gCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTAATTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTa                                                                            <  1:3610802/93‑1 (MQ=255)
               gTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAAt                                                                          >  2:1937729/1‑93 (MQ=255)
               gTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAAt                                                                          <  2:3534604/93‑1 (MQ=255)
               gTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAAt                                                                          <  2:692669/93‑1 (MQ=255)
               gTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAAt                                                                          <  1:409546/93‑1 (MQ=255)
                tGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATc                                                                         <  1:5117116/93‑1 (MQ=255)
                  aCTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCAt                                                                       <  2:4067406/93‑1 (MQ=255)
                   cTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCAtt                                                                      >  1:6288842/1‑93 (MQ=255)
                   cTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCAtt                                                                      >  2:3390816/1‑93 (MQ=255)
                   cTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCAtt                                                                      >  2:4691698/1‑93 (MQ=255)
                   cTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCAtt                                                                      <  1:1898576/93‑1 (MQ=255)
                    ttGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTTGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTg                                                                     >  2:2523810/1‑93 (MQ=255)
                    ttGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTg                                                                     <  2:6001209/93‑1 (MQ=255)
                    ttGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTg                                                                     >  2:1758038/1‑93 (MQ=255)
                     tGGTATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTga                                                                    <  2:5514988/93‑1 (MQ=255)
                     tGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTga                                                                    <  1:3570365/93‑1 (MQ=255)
                     tGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTga                                                                    >  2:6182898/1‑93 (MQ=255)
                        gATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGAc                                                                 <  1:5997211/93‑1 (MQ=255)
                        gATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGAc                                                                 <  1:2644181/93‑1 (MQ=255)
                         aTCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACg                                                                >  2:2674226/1‑93 (MQ=255)
                          tCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGc                                                               >  2:6281326/1‑93 (MQ=255)
                            tGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCtt                                                             >  1:2037217/1‑93 (MQ=255)
                            tGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGAtt                                                             >  1:6027886/1‑93 (MQ=255)
                             gAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTc                                                            <  2:3432236/93‑1 (MQ=255)
                              aaTACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCa                                                           >  2:5130358/1‑93 (MQ=255)
                                tACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCAtc                                                         >  1:4755513/1‑93 (MQ=255)
                                tACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCAtc                                                         >  2:445538/1‑93 (MQ=255)
                                   tctGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTcc                                                      >  1:1854375/1‑93 (MQ=255)
                                   tctGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTcc                                                      <  1:4246967/93‑1 (MQ=255)
                                   tctGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTcc                                                      <  1:21954/93‑1 (MQ=255)
                                    ctGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCt                                                     <  2:3857898/93‑1 (MQ=255)
                                     tGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTg                                                    >  1:3104224/1‑93 (MQ=255)
                                       cccACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGAc                                                  <  2:3293656/93‑1 (MQ=255)
                                          aCGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGAt                                               <  1:1001842/93‑1 (MQ=255)
                                           cGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATc                                              <  1:4579273/93‑1 (MQ=255)
                                            gATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCg                                             >  1:1453522/1‑93 (MQ=255)
                                            gATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCg                                             >  2:1436367/1‑93 (MQ=255)
                                            gATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCg                                             >  2:5750700/1‑93 (MQ=255)
                                             aTTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCgg                                            >  1:5325453/1‑93 (MQ=255)
                                             aTTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCgg                                            >  2:2326798/1‑93 (MQ=255)
                                                gtgGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCaa                                         >  2:3310674/1‑93 (MQ=255)
                                                 tgGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAAt                                        <  1:1109190/93‑1 (MQ=255)
                                                 tgGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAAt                                        <  1:6112900/93‑1 (MQ=255)
                                                 tgGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAAt                                        >  1:4632967/1‑93 (MQ=255)
                                                 tgGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAAt                                        <  2:6239546/93‑1 (MQ=255)
                                                  ggCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATg                                       >  2:2146970/1‑93 (MQ=255)
                                                   gCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGt                                      <  1:826978/93‑1 (MQ=255)
                                                   gCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGt                                      >  2:6079582/1‑93 (MQ=255)
                                                   gCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGt                                      >  1:3509972/1‑93 (MQ=255)
                                                   gCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGt                                      >  1:4500837/1‑93 (MQ=255)
                                                   gCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGt                                      <  1:934687/93‑1 (MQ=255)
                                                     gTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTg                                    <  2:4509803/93‑1 (MQ=255)
                                                      tACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGa                                   >  2:1154955/1‑93 (MQ=255)
                                                      tACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGa                                   >  1:607877/1‑93 (MQ=255)
                                                      tACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGa                                   >  1:1930945/1‑93 (MQ=255)
                                                       aCTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGAc                                  >  2:2119091/1‑93 (MQ=255)
                                                         ttGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGAccc                                <  1:1811228/93‑1 (MQ=255)
                                                         ttGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGAccc                                >  1:971913/1‑93 (MQ=255)
                                                           ggCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCt                              <  1:561851/93‑1 (MQ=255)
                                                              aCGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTc                           >  2:2980028/1‑93 (MQ=255)
                                                              aCGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTc                           >  1:3680525/1‑93 (MQ=255)
                                                                   aTGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTccc                      >  2:578878/1‑93 (MQ=255)
                                                                    tGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCt                     >  1:2754581/1‑93 (MQ=255)
                                                                    tGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCt                     >  1:5478948/1‑93 (MQ=255)
                                                                       ttgttATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCa                  <  1:5021381/93‑1 (MQ=255)
                                                                       ttgttATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCa                  >  1:3743074/1‑93 (MQ=255)
                                                                       ttgttATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCa                  <  2:5732083/93‑1 (MQ=255)
                                                                        tgttATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCaa                 <  2:1367240/93‑1 (MQ=255)
                                                                        tgttATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCaa                 <  2:562140/93‑1 (MQ=255)
                                                                         gttATTCGCACCAATAAATTCAGTCATCATGTAATCATTTAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAAc                <  2:2049551/93‑1 (MQ=255)
                                                                         gttATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAAc                <  1:2454408/93‑1 (MQ=255)
                                                                            aTTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGc             >  2:2238439/1‑93 (MQ=255)
                                                                             ttCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCa            >  2:5171685/1‑93 (MQ=255)
                                                                              tCGCACCAATAAATTCAGTCATCATGTAATCATTGATACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCaa           <  1:3115781/93‑1 (MQ=255)
                                                                              tCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCaa           <  2:2613074/93‑1 (MQ=255)
                                                                                gCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATc         <  2:4788240/93‑1 (MQ=255)
                                                                                  aCCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGc       >  2:1280764/1‑93 (MQ=255)
                                                                                  aCCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGc       >  1:1493334/1‑93 (MQ=255)
                                                                                   ccAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCt      >  2:5859844/1‑93 (MQ=255)
                                                                                   ccAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCt      >  2:706308/1‑93 (MQ=255)
                                                                                    cAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCtt     >  1:2871566/1‑93 (MQ=255)
                                                                                    cAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCtt     >  2:1573765/1‑93 (MQ=255)
                                                                                     aaTAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCttt    >  1:4213401/1‑93 (MQ=255)
                                                                                     aaTAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCttt    >  2:4332099/1‑93 (MQ=255)
                                                                                       tAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCTTTCa  >  1:5747/1‑93 (MQ=255)
                                                                                          |                                                                                         
TACGTGCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCTTTCA  >  NC_000913/1751086‑1751265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTTTACGTGCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCTTTCAGG  >  NC_000913/1751083‑1751267
                                                                                             |                                                                                           
CTTTACGTGCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAA                                                                                             >  SRR4302533.2276597/1‑94 (MQ=60)
 TTTACGTGCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAAT                                                                                            <  SRR4302533.3952783/94‑1 (MQ=60)
 TTTACGTGCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAAT                                                                                            >  SRR4302533.2612857/1‑94 (MQ=60)
  TTACGTGCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATT                                                                                           >  SRR4302533.302458/1‑94 (MQ=60)
  TTACGTGCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATT                                                                                           <  SRR4302533.4851810/94‑1 (MQ=60)
   TACGTGCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTC                                                                                          >  SRR4302533.3908078/1‑94 (MQ=60)
     CGTGCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAG                                                                                        >  SRR4302533.3330847/1‑94 (MQ=60)
     CGTGCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAG                                                                                        <  SRR4302533.1559002/94‑1 (MQ=60)
      GTGCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGT                                                                                       >  SRR4302533.93637/1‑94 (MQ=60)
      GTGCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGT                                                                                       <  SRR4302533.5384546/94‑1 (MQ=60)
       TGNTGTNNAGCGTGACTTGGGNNCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTC                                                                                      <  SRR4302533.6132095/94‑1 (MQ=60)
       TGCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGTCATGGTTGTTATTCGCACCAATAAATTCAGTC                                                                                      >  SRR4302533.679861/1‑94 (MQ=60)
       TGCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTC                                                                                      >  SRR4302533.3992686/1‑94 (MQ=60)
        GCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCA                                                                                     <  SRR4302533.1208974/94‑1 (MQ=60)
         CTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTTGCACCAATAAATTCACTCAT                                                                                    >  SRR4302533.269535/1‑94 (MQ=60)
           GTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGTCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCA                                                                                  <  SRR4302533.2798692/94‑1 (MQ=60)
           GTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCA                                                                                  <  SRR4302533.5176946/94‑1 (MQ=60)
            TCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCAT                                                                                 <  SRR4302533.412592/94‑1 (MQ=60)
             CCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATG                                                                                <  SRR4302533.2015181/94‑1 (MQ=60)
              CAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGT                                                                               >  SRR4302533.4922849/1‑94 (MQ=60)
               AGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTA                                                                              >  SRR4302533.4864605/1‑94 (MQ=60)
                GCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTAATTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAA                                                                             <  SRR4302533.3633025/94‑1 (MQ=60)
                 CGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAAT                                                                            <  SRR4302533.2072394/94‑1 (MQ=60)
                 CGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAAT                                                                            <  SRR4302533.674881/94‑1 (MQ=60)
                 CGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAAT                                                                            <  SRR4302533.4639689/94‑1 (MQ=60)
                  GTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATC                                                                           <  SRR4302533.412003/94‑1 (MQ=60)
                  GTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATC                                                                           >  SRR4302533.3820995/1‑94 (MQ=60)
                  GTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATC                                                                           >  SRR4302533.3126252/1‑94 (MQ=60)
                   TGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCA                                                                          <  SRR4302533.5148039/94‑1 (MQ=60)
                   TGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCA                                                                          >  SRR4302533.2442087/1‑94 (MQ=60)
                   TGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCA                                                                          <  SRR4302533.4580121/94‑1 (MQ=60)
                   TGACTTGGGATCTGAATACTCCGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCA                                                                          <  SRR4302533.5163670/94‑1 (MQ=60)
                    GACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCAT                                                                         <  SRR4302533.1215892/94‑1 (MQ=60)
                     ACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATT                                                                        >  SRR4302533.6326354/1‑94 (MQ=60)
                      CTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTG                                                                       <  SRR4302533.1910167/94‑1 (MQ=60)
                        TGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAG                                                                     <  SRR4302533.707480/94‑1 (MQ=60)
                        TGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAG                                                                     >  SRR4302533.3105389/1‑94 (MQ=60)
                        TGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAG                                                                     <  SRR4302533.4966667/94‑1 (MQ=60)
                        TGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAG                                                                     <  SRR4302533.3592369/94‑1 (MQ=60)
                         GGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGA                                                                    <  SRR4302533.4034906/94‑1 (MQ=60)
                          GGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGAC                                                                   <  SRR4302533.1887827/94‑1 (MQ=60)
                           GATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACG                                                                  <  SRR4302533.2660428/94‑1 (MQ=60)
                           GATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACG                                                                  <  SRR4302533.6033533/94‑1 (MQ=60)
                              CTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTT                                                               >  SRR4302533.2049779/1‑94 (MQ=60)
                              CTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGATT                                                               >  SRR4302533.6064414/1‑94 (MQ=60)
                               TGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTC                                                              >  SRR4302533.1087820/1‑94 (MQ=60)
                                 AATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCAT                                                            <  SRR4302533.575457/94‑1 (MQ=60)
                                  ATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATC                                                           >  SRR4302533.4784370/1‑94 (MQ=60)
                                     CTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCC                                                        >  SRR4302533.1865682/1‑94 (MQ=60)
                                      TCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCT                                                       <  SRR4302533.22100/94‑1 (MQ=60)
                                      TCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCT                                                       <  SRR4302533.4272601/94‑1 (MQ=60)
                                       CTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTG                                                      >  SRR4302533.3123307/1‑94 (MQ=60)
                                       CTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTG                                                      >  SRR4302533.5503942/1‑94 (MQ=60)
                                         GCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGAC                                                    <  SRR4302533.2539003/94‑1 (MQ=60)
                                             ACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATC                                                <  SRR4302533.1007692/94‑1 (MQ=60)
                                             ACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATA                                                <  SRR4302533.354687/94‑1 (MQ=60)
                                              CGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCG                                               >  SRR4302533.1462289/1‑94 (MQ=60)
                                              CGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCG                                               <  SRR4302533.4607027/94‑1 (MQ=60)
                                              CGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCG                                               <  SRR4302533.6038242/94‑1 (MQ=60)
                                               GATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGG                                              >  SRR4302533.5357366/1‑94 (MQ=60)
                                                 TTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCA                                            <  SRR4302533.968570/94‑1 (MQ=60)
                                                  TGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAA                                           <  SRR4302533.6082638/94‑1 (MQ=60)
                                                   GTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAAT                                          <  SRR4302533.6313762/94‑1 (MQ=60)
                                                   GTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAAT                                          >  SRR4302533.4661043/1‑94 (MQ=60)
                                                    TGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATG                                         <  SRR4302533.6149793/94‑1 (MQ=60)
                                                    TGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATG                                         <  SRR4302533.1115774/94‑1 (MQ=60)
                                                     GGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGT                                        >  SRR4302533.4528100/1‑94 (MQ=60)
                                                     GGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGT                                        >  SRR4302533.3531609/1‑94 (MQ=60)
                                                      GCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTT                                       <  SRR4302533.831830/94‑1 (MQ=60)
                                                      GCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTT                                       <  SRR4302533.940176/94‑1 (MQ=60)
                                                        GTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGA                                     >  SRR4302533.611454/1‑94 (MQ=60)
                                                        GTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGA                                     >  SRR4302533.3216700/1‑94 (MQ=60)
                                                        GTACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGA                                     >  SRR4302533.1942724/1‑94 (MQ=60)
                                                         TACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGAC                                    >  SRR4302533.1485937/1‑94 (MQ=60)
                                                          ACTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACC                                   >  SRR4302533.4315579/1‑94 (MQ=60)
                                                           CTTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCC                                  >  SRR4302533.977619/1‑94 (MQ=60)
                                                            TTGGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCC                                 <  SRR4302533.1822293/94‑1 (MQ=60)
                                                              GGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTT                               <  SRR4302533.565183/94‑1 (MQ=60)
                                                              GGCACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTT                               >  SRR4302533.5465764/1‑94 (MQ=60)
                                                                CACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTC                             >  SRR4302533.3703142/1‑94 (MQ=60)
                                                                 ACGACATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCG                            >  SRR4302533.5361407/1‑94 (MQ=60)
                                                                     CATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCC                        <  SRR4302533.426258/94‑1 (MQ=60)
                                                                     CATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCC                        >  SRR4302533.4757373/1‑94 (MQ=60)
                                                                      ATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCT                       >  SRR4302533.2771472/1‑94 (MQ=60)
                                                                      ATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCT                       >  SRR4302533.3751996/1‑94 (MQ=60)
                                                                      ATGGTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCT                       >  SRR4302533.5511978/1‑94 (MQ=60)
                                                                         GTTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCA                    >  SRR4302533.3766031/1‑94 (MQ=60)
                                                                          TTGTTATTCTCAACAATAAATTCAGTCAGCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAA                   <  SRR4302533.1667231/94‑1 (MQ=60)
                                                                          TTGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAA                   <  SRR4302533.5051791/94‑1 (MQ=60)
                                                                           TGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCTTCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAAC                  <  SRR4302533.3399802/94‑1 (MQ=60)
                                                                           TGTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAAC                  <  SRR4302533.368395/94‑1 (MQ=60)
                                                                            GTTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACC                 <  SRR4302533.2469557/94‑1 (MQ=60)
                                                                             TTATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCG                <  SRR4302533.5144339/94‑1 (MQ=60)
                                                                              TATTCGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGC               <  SRR4302533.2751058/94‑1 (MQ=60)
                                                                                 TCGCACCAATAAATTCAGTCATCATGTAATCATTGATACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAAT            <  SRR4302533.3134937/94‑1 (MQ=60)
                                                                                  CGCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATC           >  SRR4302533.3608753/1‑94 (MQ=60)
                                                                                   GCACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCG          >  SRR4302533.532889/1‑94 (MQ=60)
                                                                                    CACCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGC         >  SRR4302533.1502338/1‑94 (MQ=60)
                                                                                      CCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCTT       >  SRR4302533.2889234/1‑94 (MQ=60)
                                                                                      CCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCTT       >  SRR4302533.2889236/1‑94 (MQ=60)
                                                                                      CCAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCTT       >  SRR4302533.5573373/1‑94 (MQ=60)
                                                                                       CAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCTTT      >  SRR4302533.4238891/1‑94 (MQ=60)
                                                                                       CAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCTTT      <  SRR4302533.1855358/94‑1 (MQ=60)
                                                                                       CAATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCTTT      >  SRR4302533.1934321/1‑94 (MQ=60)
                                                                                        AATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCTTTC     <  SRR4302533.5967271/94‑1 (MQ=60)
                                                                                         ATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCTTTCA    >  SRR4302533.5802/1‑94 (MQ=60)
                                                                                         ATAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCTTTCA    <  SRR4302533.1148311/94‑1 (MQ=60)
                                                                                          TAAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCTTTCAG   >  SRR4302533.3084585/1‑94 (MQ=60)
                                                                                           AAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCTTTCAGG  <  SRR4302533.4121519/94‑1 (MQ=60)
                                                                                           AAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCTTTCAGG  <  SRR4302533.5552670/94‑1 (MQ=60)
                                                                                           AAATTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCTTTCAGG  <  SRR4302533.2175325/94‑1 (MQ=60)
                                                                                             |                                                                                           
CTTTACGTGCTGTCCAGCGTGACTTGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCTTTCAGG  >  NC_000913/1751083‑1751267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑