Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,757,874 T→C H225R (CAT→CGT)  b1680 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,757,8740TC99.0% 649.6 / ‑2.9 202H225R (CAT→CGT) b1680hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base C (120/80);  total (120/82)
Fisher's exact test for biased strand distribution p-value = 1.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.40e-01

Only 100 of 209 total aligned reads displayed.

TCATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTATGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACTTT  >  NC_000913/1757782‑1757962
                                                                                            |                                                                                        
tCATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTAt                                                                                          >  1:1082067/1‑93 (MQ=255)
  aTAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGc                                                                                        <  1:8227627/93‑1 (MQ=255)
     gAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCTAATTCCGGTGGGGCCATACAGTTTACGCCCg                                                                                     >  1:2733735/1‑93 (MQ=255)
     gAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCg                                                                                     >  2:4460907/1‑93 (MQ=255)
     gAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAAGTCCGGTGGGGCCATACAGTTTATGCCCg                                                                                     <  1:6809/93‑1 (MQ=255)
     gAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGACAATTCCGGTGGGGCCATACAGTTTACGCCCg                                                                                     >  2:3625921/1‑93 (MQ=255)
       aCCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGga                                                                                   <  2:5886165/93‑1 (MQ=255)
       aCCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGga                                                                                   <  1:2127261/93‑1 (MQ=255)
        ccGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGgag                                                                                  <  1:5141452/93‑1 (MQ=255)
         cGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGgaga                                                                                 <  2:5190885/93‑1 (MQ=255)
          gCCCCCTTCCCACGGCGGCATCTCCTGCCACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGgaggc                                                                                >  1:5815618/1‑91 (MQ=255)
            ccccTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGcaca                                                                              >  2:6404049/1‑93 (MQ=255)
            ccccTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAaca                                                                              >  1:3291128/1‑93 (MQ=255)
             cccTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGGCATACAGTTTACGCCCGGAGAacac                                                                             >  2:70534/1‑93 (MQ=255)
             cccTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAacac                                                                             >  1:9546545/1‑93 (MQ=255)
             cccTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAacac                                                                             >  2:9447999/1‑93 (MQ=255)
             cccTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAacac                                                                             <  1:7527337/93‑1 (MQ=255)
               cTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGt                                                                           <  2:1132909/93‑1 (MQ=255)
               cTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGt                                                                           >  2:5480045/1‑93 (MQ=255)
                 tCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTaa                                                                         >  2:4147969/1‑93 (MQ=255)
                     ccggcggCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGt                                                                     <  1:2898521/92‑1 (MQ=255)
                     aCGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGt                                                                     >  1:9122328/1‑93 (MQ=255)
                      cggcggCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTc                                                                    <  2:1098956/93‑1 (MQ=255)
                        gcggcATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGc                                                                  >  1:1212159/1‑93 (MQ=255)
                         cggcATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCa                                                                 <  1:148627/93‑1 (MQ=255)
                          ggcATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCaa                                                                >  1:7510190/1‑93 (MQ=255)
                          cgcATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCaa                                                                <  2:3384653/92‑1 (MQ=255)
                            cATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATc                                                              >  2:7748875/1‑93 (MQ=255)
                              tctcCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCa                                                            >  1:1418472/1‑93 (MQ=255)
                              tctcCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCa                                                            >  2:1078591/1‑93 (MQ=255)
                              tctcCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCa                                                            >  2:2875075/1‑93 (MQ=255)
                              tctcCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCa                                                            <  2:9147712/93‑1 (MQ=255)
                              tctcCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCa                                                            >  2:191312/1‑93 (MQ=255)
                                   tGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTCAAAGTCGCAATCCAGCGcc                                                       >  2:1627434/1‑93 (MQ=255)
                                   tGCAACAAGGCTTCTGTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGcc                                                       <  1:9766322/93‑1 (MQ=255)
                                    gCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCt                                                      >  2:8776495/1‑93 (MQ=255)
                                     caacaaGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTg                                                     <  2:969329/93‑1 (MQ=255)
                                      aacaaGTCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGa                                                    <  2:4230747/93‑1 (MQ=255)
                                      aacaaGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGa                                                    >  2:303735/1‑93 (MQ=255)
                                       acaaGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGaa                                                   >  1:2416900/1‑93 (MQ=255)
                                       acaaGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGaa                                                   >  1:8036973/1‑93 (MQ=255)
                                       acaaGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGaa                                                   >  1:8192811/1‑93 (MQ=255)
                                         aaGGCTTCTTTCACATAAAGAATGCCCATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACa                                                 <  1:8266578/93‑1 (MQ=255)
                                          aGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACAt                                                >  1:2602444/1‑93 (MQ=255)
                                           ggCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATc                                               >  2:1195335/1‑93 (MQ=255)
                                            gCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATcc                                              >  2:9880229/1‑93 (MQ=255)
                                            gCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATcc                                              <  1:8797292/93‑1 (MQ=255)
                                            gCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATcc                                              <  1:9463159/93‑1 (MQ=255)
                                             cttcttTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCa                                             <  1:230125/93‑1 (MQ=255)
                                              ttcttTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCAc                                            <  2:9254430/93‑1 (MQ=255)
                                              ttcttTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCAc                                            >  2:9050118/1‑93 (MQ=255)
                                              ttcttTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCAc                                            >  2:10019690/1‑93 (MQ=255)
                                              ttcttTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCAc                                            <  2:595743/93‑1 (MQ=255)
                                              ttcttTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCAc                                            >  1:5352945/1‑93 (MQ=255)
                                              ttcttTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCAc                                            >  2:2685413/1‑93 (MQ=255)
                                               tcttTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCAAGCGCCTGAACATCCAcg                                           >  1:2191658/1‑92 (MQ=255)
                                                cttTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATTCACCg                                          >  2:7270398/1‑93 (MQ=255)
                                                 tttCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCgg                                         >  1:3428937/1‑93 (MQ=255)
                                                  ttCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGga                                        >  1:1302530/1‑93 (MQ=255)
                                                   tCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGgat                                       >  1:2644826/1‑93 (MQ=255)
                                                   tCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGgat                                       <  1:3068232/93‑1 (MQ=255)
                                                    cacaTAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGgatg                                      <  2:8283816/93‑1 (MQ=255)
                                                     acaTAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGgatga                                     <  1:7251400/93‑1 (MQ=255)
                                                     acaTAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGgatga                                     <  1:3843308/93‑1 (MQ=255)
                                                           aGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGACGATGCATc                               <  1:6846291/93‑1 (MQ=255)
                                                            gAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCa                              >  2:2012622/1‑93 (MQ=255)
                                                            gAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCa                              >  2:8479914/1‑93 (MQ=255)
                                                             aaTGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCAc                             <  1:4912370/93‑1 (MQ=255)
                                                                   aaTTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTg                       >  2:6182569/1‑93 (MQ=255)
                                                                   aaTTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTg                       >  1:7849183/1‑93 (MQ=255)
                                                                   aaTTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTg                       >  2:6626008/1‑93 (MQ=255)
                                                                     ttCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAg                     >  2:8645682/1‑93 (MQ=255)
                                                                     ttCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAg                     >  1:643031/1‑93 (MQ=255)
                                                                      tCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAgc                    >  1:6931076/1‑93 (MQ=255)
                                                                        cGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAgcgc                  >  1:7018877/1‑93 (MQ=255)
                                                                         ggTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGcc                 >  2:7608133/1‑93 (MQ=255)
                                                                          gTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCa                <  2:7354320/93‑1 (MQ=255)
                                                                          gTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCa                >  2:5678784/1‑93 (MQ=255)
                                                                           tGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCAt               <  2:1684870/93‑1 (MQ=255)
                                                                           tGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCAt               <  2:240672/93‑1 (MQ=255)
                                                                            ggggCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATc              >  1:9149606/1‑93 (MQ=255)
                                                                             gggCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATcc             >  2:3182409/1‑93 (MQ=255)
                                                                             gggCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATcc             >  2:7683911/1‑93 (MQ=255)
                                                                               gCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCGCCGCCTGAGCGCCATccac           <  1:217458/93‑1 (MQ=255)
                                                                               gCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCAGCGCCTGAGCGCCATccac           >  1:6739292/1‑93 (MQ=255)
                                                                                ccATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATccacc          <  2:6655956/93‑1 (MQ=255)
                                                                                 cATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATccacca         >  2:7126997/1‑93 (MQ=255)
                                                                                 cATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATccacca         <  1:9884576/93‑1 (MQ=255)
                                                                                 cATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATccacca         <  1:191312/93‑1 (MQ=255)
                                                                                 cATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATccacca         >  1:1655271/1‑93 (MQ=255)
                                                                                  aTACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAg        >  1:7249693/1‑93 (MQ=255)
                                                                                  aTACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAg        >  1:9485427/1‑93 (MQ=255)
                                                                                   tACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGc       >  2:7075946/1‑93 (MQ=255)
                                                                                    aCAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCa      <  2:6927954/93‑1 (MQ=255)
                                                                                      aGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACt    <  1:4269183/93‑1 (MQ=255)
                                                                                      aGTTTACGCCCGGAGAACACGTAAAAGTCGCAATACAGCGCCTGCACATCCACCGGCTAATGCATCACCGCCTGAGAGCCGCCCACCAACACt    >  1:5690062/1‑93 (MQ=255)
                                                                                       gTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACtt   >  2:4389696/1‑93 (MQ=255)
                                                                                       gTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACtt   >  2:9679660/1‑93 (MQ=255)
                                                                                        tttACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACttt  >  1:2383712/1‑93 (MQ=255)
                                                                                        tttACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACttt  >  1:4106708/1‑93 (MQ=255)
                                                                                            |                                                                                        
TCATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTATGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACTTT  >  NC_000913/1757782‑1757962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTATGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACTTTTGCGC  >  NC_000913/1757781‑1757967
                                                                                             |                                                                                             
ATCATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTAT                                                                                               >  SRR4302540.1093149/1‑94 (MQ=60)
ATCATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTAC                                                                                               <  SRR4302540.2653310/94‑1 (MQ=60)
ATCATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTAC                                                                                               <  SRR4302540.581929/94‑1 (MQ=60)
 TCATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACG                                                                                              <  SRR4302540.2520063/94‑1 (MQ=60)
 TCATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACG                                                                                              <  SRR4302540.8051206/94‑1 (MQ=60)
   ATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCC                                                                                            <  SRR4302540.8323267/94‑1 (MQ=60)
   ATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCC                                                                                            <  SRR4302540.8323268/94‑1 (MQ=60)
   ATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCC                                                                                            >  SRR4302540.7604715/1‑94 (MQ=60)
     AGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCTAATTCCGGTGGGGCCATACAGTTTACGCCCG                                                                                          >  SRR4302540.2763236/1‑94 (MQ=60)
     AGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCG                                                                                          >  SRR4302540.8585923/1‑94 (MQ=60)
      GAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGG                                                                                         <  SRR4302540.7752650/94‑1 (MQ=60)
      GAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAAGTCCGGTGGGGCCATACAGTTTATGCCCGG                                                                                         <  SRR4302540.6885/94‑1 (MQ=60)
        ACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAG                                                                                       <  SRR4302540.2150088/94‑1 (MQ=60)
         CCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGA                                                                                      <  SRR4302540.5197140/94‑1 (MQ=60)
         CCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGA                                                                                      >  SRR4302540.4660876/1‑94 (MQ=60)
          CGCCCCCTTCCCACGGCGGCATCTCCTGCCACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGgc                                                                                     >  SRR4302540.5879899/1‑92 (MQ=60)
            CCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACA                                                                                   >  SRR4302540.3327136/1‑94 (MQ=60)
             CCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACAC                                                                                  >  SRR4302540.9659488/1‑94 (MQ=60)
              CCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACG                                                                                 >  SRR4302540.1404335/1‑94 (MQ=60)
              CCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACG                                                                                 <  SRR4302540.2196217/94‑1 (MQ=60)
              CCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACG                                                                                 <  SRR4302540.7611578/94‑1 (MQ=60)
               CCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGT                                                                                <  SRR4302540.5026817/94‑1 (MQ=60)
               CCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGCGGCCATACAGTTTACGCCCGGAGAACACGT                                                                                >  SRR4302540.2218996/1‑94 (MQ=60)
                CTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTA                                                                               >  SRR4302540.4282399/1‑94 (MQ=60)
                CTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTA                                                                               <  SRR4302540.5713741/94‑1 (MQ=60)
                 TTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAA                                                                              >  SRR4302540.8289543/1‑94 (MQ=60)
                  TCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAA                                                                             <  SRR4302540.7094952/94‑1 (MQ=60)
                   CCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCGCTACAGTTTACGCCCGGAGAcgagtgacac                                                                            >  SRR4302540.7060778/1‑84 (MQ=60)
                    CCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAG                                                                           <  SRR4302540.2038379/94‑1 (MQ=60)
                     CACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGT                                                                          >  SRR4302540.243000/1‑94 (MQ=60)
                     CACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGT                                                                          >  SRR4302540.9231982/1‑94 (MQ=60)
                      CCGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTC                                                                         <  SRR4302540.2930136/94‑1 (MQ=60)
                      ACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTC                                                                         >  SRR4302540.7334548/1‑94 (MQ=60)
                        GGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGC                                                                       >  SRR4302540.1224588/1‑94 (MQ=60)
                        GGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGC                                                                       >  SRR4302540.9725770/1‑94 (MQ=60)
                        GGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGC                                                                       >  SRR4302540.6045195/1‑94 (MQ=60)
                          CGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAA                                                                     <  SRR4302540.150161/94‑1 (MQ=60)
                          CGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAA                                                                     >  SRR4302540.7594137/1‑94 (MQ=60)
                          CGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAA                                                                     >  SRR4302540.2421440/1‑94 (MQ=60)
                           GGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAAT                                                                    <  SRR4302540.924224/94‑1 (MQ=60)
                           GGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCACACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAAT                                                                    <  SRR4302540.1795906/94‑1 (MQ=60)
                              ATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCA                                                                 >  SRR4302540.3974242/1‑94 (MQ=60)
                              ATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCA                                                                 >  SRR4302540.1433807/1‑94 (MQ=60)
                               TCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAG                                                                <  SRR4302540.691825/94‑1 (MQ=60)
                               TCTCCTGCAACAAGGATTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCTTACAGTTTACCCCGGGAGAAAACGAAAAAGGCGCAATCCAG                                                                >  SRR4302540.4442115/1‑94 (MQ=60)
                                    TGCAACAAGGCTTCTGTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCT                                                           <  SRR4302540.9880853/94‑1 (MQ=60)
                                     GCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTG                                                          <  SRR4302540.6847537/94‑1 (MQ=60)
                                       AACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAA                                                        >  SRR4302540.2442944/1‑94 (MQ=60)
                                       AACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAA                                                        >  SRR4302540.8130382/1‑94 (MQ=60)
                                       AACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAA                                                        >  SRR4302540.8288008/1‑94 (MQ=60)
                                          AAGGCTTCTTTCACATAAAGAATGCCCATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACAT                                                     <  SRR4302540.8362630/94‑1 (MQ=60)
                                          AAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACAT                                                     >  SRR4302540.2932734/1‑94 (MQ=60)
                                          AAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACAT                                                     >  SRR4302540.2630514/1‑94 (MQ=60)
                                           AGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGTCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATC                                                    <  SRR4302540.4324303/94‑1 (MQ=60)
                                            GGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCC                                                   <  SRR4302540.3225145/94‑1 (MQ=60)
                                             GCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCA                                                  <  SRR4302540.9575434/94‑1 (MQ=60)
                                             GCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCA                                                  <  SRR4302540.8899015/94‑1 (MQ=60)
                                              CTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCAC                                                 >  SRR4302540.5411714/1‑94 (MQ=60)
                                              CTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCAC                                                 <  SRR4302540.232350/94‑1 (MQ=60)
                                              CTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCAC                                                 >  SRR4302540.3446615/1‑94 (MQ=60)
                                               TTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCAAGCGCCTGAACATCCACG                                                >  SRR4302540.2215329/1‑94 (MQ=60)
                                                 CTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGG                                              >  SRR4302540.9576539/1‑94 (MQ=60)
                                                 CTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGG                                              >  SRR4302540.3466196/1‑94 (MQ=60)
                                                  TTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGA                                             >  SRR4302540.1316040/1‑94 (MQ=60)
                                                  TTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGA                                             >  SRR4302540.1315717/1‑94 (MQ=60)
                                                   TTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGAT                                            >  SRR4302540.2673517/1‑94 (MQ=60)
                                                    TCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATG                                           <  SRR4302540.2404529/94‑1 (MQ=60)
                                                    TCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATG                                           <  SRR4302540.3101893/94‑1 (MQ=60)
                                                      ACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGAT                                         <  SRR4302540.3885107/94‑1 (MQ=60)
                                                      ACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGAT                                         <  SRR4302540.7332318/94‑1 (MQ=60)
                                                      ACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGAT                                         <  SRR4302540.7332316/94‑1 (MQ=60)
                                                       CATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATG                                        <  SRR4302540.2928531/94‑1 (MQ=60)
                                                        ATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCTCCTGAACATCCACCGGATGATGC                                       >  SRR4302540.6532647/1‑94 (MQ=60)
                                                        ATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGC                                       >  SRR4302540.2698983/1‑94 (MQ=60)
                                                         TAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCA                                      >  SRR4302540.7185108/1‑94 (MQ=60)
                                                          AAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCAT                                     >  SRR4302540.8050939/1‑94 (MQ=60)
                                                           AAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATC                                    <  SRR4302540.9236637/94‑1 (MQ=60)
                                                            AGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCA                                   <  SRR4302540.10042248/94‑1 (MQ=60)
                                                            AGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGACGATGCATCA                                   <  SRR4302540.6922136/94‑1 (MQ=60)
                                                              AATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACC                                 <  SRR4302540.4965265/94‑1 (MQ=60)
                                                                TGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGC                               >  SRR4302540.1179775/1‑94 (MQ=60)
                                                                 GCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCC                              <  SRR4302540.2666120/94‑1 (MQ=60)
                                                                   CAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTG                            >  SRR4302540.529859/1‑94 (MQ=60)
                                                                   CAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTG                            >  SRR4302540.7940895/1‑94 (MQ=60)
                                                                    AATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGA                           >  SRR4302540.38385/1‑94 (MQ=60)
                                                                     ATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAG                          >  SRR4302540.649882/1‑94 (MQ=60)
                                                                      TTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGC                         >  SRR4302540.7008205/1‑94 (MQ=60)
                                                                       TCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCG                        >  SRR4302540.1546296/1‑94 (MQ=60)
                                                                        CCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGC                       >  SRR4302540.7096834/1‑94 (MQ=60)
                                                                          GGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCA                     >  SRR4302540.8257316/1‑94 (MQ=60)
                                                                            TGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATC                   >  SRR4302540.9259478/1‑94 (MQ=60)
                                                                             GGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCAGCGCCTGAGCGCCATCC                  >  SRR4302540.9888602/1‑94 (MQ=60)
                                                                              GGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCA                 >  SRR4302540.2199620/1‑94 (MQ=60)
                                                                               GTCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCAC                <  SRR4302540.9508141/94‑1 (MQ=60)
                                                                               GGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCAGCGCCTGAGCGCCATCCAC                >  SRR4302540.6813738/1‑94 (MQ=60)
                                                                               GGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCAC                <  SRR4302540.5789382/94‑1 (MQ=60)
                                                                                GCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCGCCGCCTGAGCGCCATCCACC               <  SRR4302540.219574/94‑1 (MQ=60)
                                                                                GCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACC               <  SRR4302540.1434537/94‑1 (MQ=60)
                                                                                 CCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCA              >  SRR4302540.1673309/1‑94 (MQ=60)
                                                                                  CATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAG             <  SRR4302540.9999995/94‑1 (MQ=60)
                                                                                  CATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAG             >  SRR4302540.9597890/1‑94 (MQ=60)
                                                                                  CATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAG             <  SRR4302540.193216/94‑1 (MQ=60)
                                                                                  CATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAG             >  SRR4302540.7330589/1‑94 (MQ=60)
                                                                                  CATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCACCACCGCCTGAGCGCCATCCACCAG             >  SRR4302540.9050008/1‑94 (MQ=60)
                                                                                   ATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGC            <  SRR4302540.724136/94‑1 (MQ=60)
                                                                                      CAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATACAGCGCCTGCACATCCACCGGCTAATGCATCACCGCCTGAGAGCCGCCCACCAACACT         >  SRR4302540.5752700/1‑94 (MQ=60)
                                                                                       AGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACTT        >  SRR4302540.6926262/1‑94 (MQ=60)
                                                                                       AGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACTT        <  SRR4302540.4315619/94‑1 (MQ=60)
                                                                                        GTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACTTT       >  SRR4302540.4151014/1‑94 (MQ=60)
                                                                                        GTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACTTT       >  SRR4302540.2409395/1‑94 (MQ=60)
                                                                                        GTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACTTT       >  SRR4302540.9612995/1‑94 (MQ=60)
                                                                                        GTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACTTT       <  SRR4302540.2498164/94‑1 (MQ=60)
                                                                                           TACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACTTTTGC    >  SRR4302540.635255/1‑94 (MQ=60)
                                                                                             CGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACTTTTGCGC  >  SRR4302540.9775359/1‑94 (MQ=60)
                                                                                             |                                                                                             
ATCATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTATGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACTTTTGCGC  >  NC_000913/1757781‑1757967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑