Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,758,261 T→C E96G (GAA→GGA)  b1680 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,758,2610TC100.0% 536.8 / NA 166E96G (GAA→GGA) b1680hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (94/72);  total (94/72)

Only 100 of 176 total aligned reads displayed.

TTAGCGTGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTTCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCATTTTCTCG  >  NC_000913/1758171‑1758350
                                                                                          |                                                                                         
ttAGCGTGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGAtccctccc                                                                                         <  1:9394650/93‑1 (MQ=255)
 tAGCGTGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCcg                                                                                        <  1:8741747/93‑1 (MQ=255)
     gtggtgCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCcgtcgt                                                                                    <  2:5723683/93‑1 (MQ=255)
      tggtgCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTg                                                                                   >  1:5454164/1‑93 (MQ=255)
      tggtgCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTg                                                                                   <  1:9220896/93‑1 (MQ=255)
      tggtgCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTg                                                                                   >  2:3020929/1‑93 (MQ=255)
       ggtgCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGc                                                                                  <  1:8305202/93‑1 (MQ=255)
         tgCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCcg                                                                                >  1:2065897/1‑93 (MQ=255)
          gCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCcgc                                                                               <  1:5416949/93‑1 (MQ=255)
          gCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCcgc                                                                               <  1:9915241/93‑1 (MQ=255)
             ccATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGa                                                                            >  2:5571500/1‑93 (MQ=255)
               aTCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGTGACCAGATTGATCCCTCCCGTCGTGCCGCGGACg                                                                          >  2:7723775/1‑93 (MQ=255)
                 ctgactgaTGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGaa                                                                        >  1:8322867/1‑93 (MQ=255)
                  tgactgaTGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAac                                                                       >  1:7277353/1‑93 (MQ=255)
                   gactgaTGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAaca                                                                      >  1:555932/1‑93 (MQ=255)
                     ctgaTGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACAcc                                                                    >  2:4912573/1‑93 (MQ=255)
                     ctgaTGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACAcc                                                                    >  2:3603180/1‑93 (MQ=255)
                     ctgaTGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACAcc                                                                    >  1:8023844/1‑93 (MQ=255)
                      tgatgatgaTGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCa                                                                   <  2:6118010/93‑1 (MQ=255)
                        atgatgatgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGCCGAACACCAGc                                                                 >  1:2214043/1‑93 (MQ=255)
                        atgatgatgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCCTCGTGCCGCGGACGAACACCAGc                                                                 >  2:5502093/1‑93 (MQ=255)
                         tgatgatgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGct                                                                >  1:1038096/1‑93 (MQ=255)
                         tgatgatgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGct                                                                >  2:3320641/1‑93 (MQ=255)
                          gatgatgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGctc                                                               <  1:8078842/93‑1 (MQ=255)
                            tgatgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCCGCTCtt                                                             >  1:2784602/1‑93 (MQ=255)
                            tgatgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCtt                                                             <  1:8479915/93‑1 (MQ=255)
                            tgatgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCtt                                                             >  2:1198617/1‑93 (MQ=255)
                             gatgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTc                                                            >  2:2163101/1‑93 (MQ=255)
                              atgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTcc                                                           <  2:24888/93‑1 (MQ=255)
                               tgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTccg                                                          >  2:8646826/1‑93 (MQ=255)
                               tgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTccg                                                          >  1:8154564/1‑93 (MQ=255)
                               tgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTccg                                                          <  2:2735019/93‑1 (MQ=255)
                               tgTTATCCCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTccg                                                          <  2:2735018/93‑1 (MQ=255)
                                gtcaTCGCCCACCCGCACGATGCTGTCGCCCGATCAATTGGCGACCAGATTGTCGCCTACCGCCGTGCCGCGGACGAACACCAGCTCTTccga                                                         <  2:6460739/90‑2 (MQ=255)
                                gTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTccgc                                                         >  1:2155075/1‑93 (MQ=255)
                                  tATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTccgccg                                                       >  1:8566448/1‑93 (MQ=255)
                                    tCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGaa                                                     >  2:2132515/1‑93 (MQ=255)
                                     cgcccgccCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAAc                                                    <  2:7797188/93‑1 (MQ=255)
                                       cccgcccgCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACgg                                                  <  2:4480762/93‑1 (MQ=255)
                                       cccgcccgCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACgg                                                  <  1:6503600/93‑1 (MQ=255)
                                       cccgcccgCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACgg                                                  >  1:2699429/1‑93 (MQ=255)
                                        ccgcccgcACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACggg                                                 >  2:4955734/1‑93 (MQ=255)
                                          gcgcgcACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCa                                               <  1:1141594/90‑1 (MQ=255)
                                          gcccgcACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCa                                               <  1:9588476/93‑1 (MQ=255)
                                          gcccgcACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCa                                               <  2:918859/93‑1 (MQ=255)
                                          gcccgcACGGGGCTGTGGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAGCACCAGCCCGTCCGCCGAACGGGCa                                               >  2:8981230/1‑93 (MQ=255)
                                           cccgcACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCAt                                              >  1:9453867/1‑93 (MQ=255)
                                           cccgcACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCAt                                              <  2:2368348/93‑1 (MQ=255)
                                            ccgcACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCAtt                                             >  2:8266384/1‑93 (MQ=255)
                                             cgcACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATta                                            >  2:3981801/1‑93 (MQ=255)
                                             cgcACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATta                                            >  2:8602274/1‑93 (MQ=255)
                                              gcACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATtaa                                           >  2:4093743/1‑93 (MQ=255)
                                              gcACGTTGCTGCTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATtaa                                           <  2:6550031/93‑1 (MQ=255)
                                                ccGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATtaata                                         <  2:3429527/92‑1 (MQ=255)
                                                aCGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATtaata                                         <  1:3679065/93‑1 (MQ=255)
                                                 cGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATtaataa                                        >  2:799437/1‑93 (MQ=255)
                                                  gTTGCTGTTGGTCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATaaa                                       <  2:1195258/93‑1 (MQ=255)
                                                   ttGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATaaaa                                      >  1:1950819/1‑92 (MQ=255)
                                                   ttGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAAc                                      <  1:3863088/93‑1 (MQ=255)
                                                   ttGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAAc                                      >  2:5673424/1‑93 (MQ=255)
                                                   ttGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCACCTCTTCCGCCGAACGGGCATTAATAAAc                                      >  1:1950818/1‑93 (MQ=255)
                                                    tGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACa                                     <  1:2257704/93‑1 (MQ=255)
                                                    tGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACa                                     >  2:3913378/1‑93 (MQ=255)
                                                     tcTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAg                                    <  1:6553275/92‑1 (MQ=255)
                                                       tGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTTCGCCGAACGGGCATCAATAAACAACg                                  >  1:7234788/1‑93 (MQ=255)
                                                        gTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGa                                 <  2:3156896/93‑1 (MQ=255)
                                                        gTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGa                                 <  2:4172920/93‑1 (MQ=255)
                                                        gTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGa                                 <  2:4172921/93‑1 (MQ=255)
                                                        gTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGa                                 <  2:4621854/93‑1 (MQ=255)
                                                         ttGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGAt                                <  1:9556101/93‑1 (MQ=255)
                                                         ttGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGAt                                <  2:3812075/93‑1 (MQ=255)
                                                         ttGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGAt                                <  1:2938930/93‑1 (MQ=255)
                                                          tGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATg                               >  1:6821450/1‑93 (MQ=255)
                                                            ccccAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGcc                             >  2:1293241/1‑93 (MQ=255)
                                                             cccAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGccc                            >  1:7355996/1‑93 (MQ=255)
                                                             cccAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGccc                            <  2:6052245/93‑1 (MQ=255)
                                                             cccAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGccc                            <  2:6052255/93‑1 (MQ=255)
                                                               cAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGc                          <  1:9262571/93‑1 (MQ=255)
                                                               cAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGc                          >  2:6321076/1‑93 (MQ=255)
                                                               cAGCTATGGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGc                          <  2:4343324/93‑1 (MQ=255)
                                                                aGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCt                         >  2:7359735/1‑93 (MQ=255)
                                                                   tATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTgc                      >  1:432899/1‑93 (MQ=255)
                                                                     ttGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTgcgc                    <  1:1607491/93‑1 (MQ=255)
                                                                        gCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACg                 <  2:3495774/93‑1 (MQ=255)
                                                                           aCCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCGGCTTGCGCACGTtc              >  1:2921648/1‑93 (MQ=255)
                                                                            ccAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTtct             <  1:4662762/93‑1 (MQ=255)
                                                                             cAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTtctc            <  1:5766254/93‑1 (MQ=255)
                                                                             cAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTtctc            <  1:9774827/93‑1 (MQ=255)
                                                                              aGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTcc           >  2:5307287/1‑93 (MQ=255)
                                                                               gATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTcct          >  1:6752321/1‑92 (MQ=255)
                                                                               gATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCa          <  1:6197680/93‑1 (MQ=255)
                                                                                aTTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCAt         <  1:1251772/93‑1 (MQ=255)
                                                                                   gATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCAtttt      >  1:9706762/1‑93 (MQ=255)
                                                                                    aTCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCATTTtc     >  2:492973/1‑93 (MQ=255)
                                                                                    aTCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCATTTtc     >  1:7426396/1‑93 (MQ=255)
                                                                                      cccTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCATTTtctc   >  2:6798732/1‑93 (MQ=255)
                                                                                      cccTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCATTTtctc   >  1:4245649/1‑93 (MQ=255)
                                                                                       ccTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCATTTTCTCg  <  1:4548928/93‑1 (MQ=255)
                                                                                       ccTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCATTTTCTCg  >  2:192180/1‑93 (MQ=255)
                                                                                       ccTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCATTTTCTCg  >  2:9464950/1‑93 (MQ=255)
                                                                                          |                                                                                         
TTAGCGTGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTTCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCATTTTCTCG  >  NC_000913/1758171‑1758350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGTTAGCGTGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTTCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCATTTTCTCGGTCG  >  NC_000913/1758169‑1758354
                                                                                            |                                                                                             
TGTTAGCGTGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCTCGCACGTTGCTGTTGCCCCAGCTATTTGCGACCAGATTGATCCCTCC                                                                                              >  SRR4302540.5336236/1‑94 (MQ=60)
TGTTAGCGTGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCC                                                                                              <  SRR4302540.8648296/94‑1 (MQ=60)
TGTTAGCGTGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCC                                                                                              <  SRR4302540.1221322/94‑1 (MQ=60)
AGTTAGCGTGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCC                                                                                              <  SRR4302540.8648297/94‑1 (MQ=60)
 GTTAGCGTGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCC                                                                                             <  SRR4302540.8059390/94‑1 (MQ=60)
 GTTAGCATGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGGTCCCTCCC                                                                                             <  SRR4302540.8762447/94‑1 (MQ=60)
  TTAGCGTGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCG                                                                                            <  SRR4302540.9506440/94‑1 (MQ=60)
   TAGCGTGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGT                                                                                           <  SRR4302540.8843221/94‑1 (MQ=60)
       GTGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTG                                                                                       >  SRR4302540.5514194/1‑94 (MQ=60)
        TGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGC                                                                                      <  SRR4302540.9331482/94‑1 (MQ=60)
         GGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCC                                                                                     <  SRR4302540.8401640/94‑1 (MQ=60)
          GTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCG                                                                                    >  SRR4302540.2088125/1‑94 (MQ=60)
          GTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCCCCCGTCGTGCCG                                                                                    >  SRR4302540.2352088/1‑94 (MQ=60)
            GCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCG                                                                                  <  SRR4302540.10031046/94‑1 (MQ=60)
            GCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCG                                                                                  <  SRR4302540.5476516/94‑1 (MQ=60)
             CTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCTCTCCCGGCGTGCCGCGG                                                                                 >  SRR4302540.8827031/1‑94 (MQ=60)
                 ATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGA                                                                             >  SRR4302540.6912496/1‑94 (MQ=60)
                  TCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAA                                                                            >  SRR4302540.8419466/1‑94 (MQ=60)
                  TCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAA                                                                            >  SRR4302540.8419467/1‑94 (MQ=60)
                   CTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAAC                                                                           >  SRR4302540.7358549/1‑94 (MQ=60)
                   CTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAAC                                                                           >  SRR4302540.7530098/1‑94 (MQ=60)
                    TGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACA                                                                          >  SRR4302540.561992/1‑94 (MQ=60)
                      ACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACC                                                                        >  SRR4302540.8117161/1‑94 (MQ=60)
                       CTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCA                                                                       >  SRR4302540.2147849/1‑94 (MQ=60)
                         GATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGCCGAACACCAGC                                                                     >  SRR4302540.2237942/1‑94 (MQ=60)
                          ATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCT                                                                    >  SRR4302540.9769559/1‑94 (MQ=60)
                          ATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCT                                                                    >  SRR4302540.1048653/1‑94 (MQ=60)
                           TGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTC                                                                   >  SRR4302540.9448784/1‑94 (MQ=60)
                            GATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCT                                                                  <  SRR4302540.8172609/94‑1 (MQ=60)
                             ATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCCGCTCTT                                                                 >  SRR4302540.2814681/1‑94 (MQ=60)
                             ATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTT                                                                 <  SRR4302540.2930701/94‑1 (MQ=60)
                              TGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTC                                                                <  SRR4302540.8578413/94‑1 (MQ=60)
                               GATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCC                                                               <  SRR4302540.5977696/94‑1 (MQ=60)
                               GATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCC                                                               >  SRR4302540.3847126/1‑94 (MQ=60)
                               GATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCC                                                               <  SRR4302540.4624482/94‑1 (MQ=60)
                                ATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCG                                                              >  SRR4302540.8249244/1‑94 (MQ=60)
                                 TGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGC                                                             >  SRR4302540.2178268/1‑94 (MQ=60)
                                 TGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGC                                                             >  SRR4302540.9880118/1‑94 (MQ=60)
                                   TTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCG                                                           >  SRR4302540.8665929/1‑94 (MQ=60)
                                      TCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAAC                                                        <  SRR4302540.4414550/94‑1 (MQ=60)
                                        GCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGG                                                      >  SRR4302540.2728563/1‑94 (MQ=60)
                                         CCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGG                                                     <  SRR4302540.6575889/94‑1 (MQ=60)
                                            GCGCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCAT                                                  <  SRR4302540.1153528/94‑1 (MQ=60)
                                            GCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGCAAGGGCAT                                                  >  SRR4302540.2759064/1‑94 (MQ=60)
                                            GCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCAT                                                  <  SRR4302540.9701655/94‑1 (MQ=60)
                                            GCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCAT                                                  >  SRR4302540.9566075/1‑94 (MQ=60)
                                             CCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATT                                                 >  SRR4302540.4998513/1‑94 (MQ=60)
                                             CCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATT                                                 <  SRR4302540.1262950/94‑1 (MQ=60)
                                                GCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAAT                                              <  SRR4302540.7457781/94‑1 (MQ=60)
                                                  CCGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAA                                            <  SRR4302540.864820/94‑1 (MQ=60)
                                                  ACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAA                                            <  SRR4302540.3719278/94‑1 (MQ=60)
                                                  ACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCCCCCGTCGGGCCGCGTGCAACCACCAGCTCTTCCCCCTGACGGGCATTAATAA                                            >  SRR4302540.3688207/1‑94 (MQ=42)
                                                   CGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAA                                           >  SRR4302540.3315876/1‑94 (MQ=60)
                                                    GTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAAC                                          <  SRR4302540.2189798/94‑1 (MQ=60)
                                                    GTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAAA                                          >  SRR4302540.1972042/1‑94 (MQ=60)
                                                    GTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCACCTCTTCCGCCGAACGGGCATTAATAAAC                                          >  SRR4302540.1972041/1‑94 (MQ=60)
                                                     TTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACA                                         <  SRR4302540.4253383/94‑1 (MQ=60)
                                                     TTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACA                                         <  SRR4302540.3905079/94‑1 (MQ=60)
                                                      TGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAG                                        >  SRR4302540.8370285/1‑94 (MQ=60)
                                                      TGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAG                                        <  SRR4302540.2281937/94‑1 (MQ=60)
                                                       TCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGC                                       <  SRR4302540.6625953/94‑1 (MQ=60)
                                                        CTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTTCGCCGAACGGGCATCAATAAACAACG                                      >  SRR4302540.7315487/1‑94 (MQ=60)
                                                          GTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGAT                                    <  SRR4302540.6666211/94‑1 (MQ=60)
                                                           TTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATG                                   <  SRR4302540.9669108/94‑1 (MQ=60)
                                                           TTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATG                                   >  SRR4302540.6341036/1‑94 (MQ=60)
                                                           TTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATG                                   <  SRR4302540.1534819/94‑1 (MQ=60)
                                                           TTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATG                                   >  SRR4302540.6896969/1‑94 (MQ=60)
                                                           TTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATG                                   <  SRR4302540.2971190/94‑1 (MQ=60)
                                                              CCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCC                                >  SRR4302540.7438031/1‑94 (MQ=60)
                                                                CCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGC                              >  SRR4302540.10019625/1‑94 (MQ=60)
                                                                 CAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCT                             <  SRR4302540.9373373/94‑1 (MQ=60)
                                                                  AGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTT                            <  SRR4302540.9275816/94‑1 (MQ=60)
                                                                  AGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTT                            >  SRR4302540.5745934/1‑94 (MQ=60)
                                                                   GCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTG                           >  SRR4302540.6864506/1‑94 (MQ=60)
                                                                    CTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGC                          >  SRR4302540.437477/1‑94 (MQ=60)
                                                                     TATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCG                         <  SRR4302540.5573512/94‑1 (MQ=60)
                                                                       TTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCA                       <  SRR4302540.1625085/94‑1 (MQ=60)
                                                                       TTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCA                       >  SRR4302540.9551156/1‑94 (MQ=60)
                                                                          GCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGT                    <  SRR4302540.9574538/94‑1 (MQ=60)
                                                                            GACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCGGCTTGCGCACGTTC                  >  SRR4302540.2953664/1‑94 (MQ=60)
                                                                              CCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTC                <  SRR4302540.4712674/94‑1 (MQ=60)
                                                                               CAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCC               <  SRR4302540.9889412/94‑1 (MQ=60)
                                                                               CAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCC               <  SRR4302540.5829921/94‑1 (MQ=60)
                                                                               CAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCC               <  SRR4302540.7374720/94‑1 (MQ=60)
                                                                                AGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCT              >  SRR4302540.6826931/1‑94 (MQ=60)
                                                                                AGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCA              >  SRR4302540.8233593/1‑94 (MQ=60)
                                                                                AGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCA              >  SRR4302540.200976/1‑94 (MQ=60)
                                                                                 GATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCAT             <  SRR4302540.6266672/94‑1 (MQ=60)
                                                                                  ATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCATT            <  SRR4302540.1264553/94‑1 (MQ=60)
                                                                                    TGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTACTAAACAGCGATGCCCGCTTGCGCACCTTCTCCATTTT          >  SRR4302540.1907257/1‑94 (MQ=60)
                                                                                    TGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCATTTT          >  SRR4302540.9820849/1‑94 (MQ=60)
                                                                                     GATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCATTTTC         >  SRR4302540.7509127/1‑94 (MQ=60)
                                                                                       TCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCATTTTCTC       >  SRR4302540.4291846/1‑94 (MQ=60)
                                                                                        CCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCATTTTCTCG      <  SRR4302540.8789768/94‑1 (MQ=60)
                                                                                         CCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCATTTTCTCGG     <  SRR4302540.4598134/94‑1 (MQ=60)
                                                                                          CTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCCTTTTCTCGAT    >  SRR4302540.891110/1‑94 (MQ=60)
                                                                                           TCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCATTTTCTCGGTC   >  SRR4302540.3094122/1‑94 (MQ=60)
                                                                                           TCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCATTTTCTCGGTC   <  SRR4302540.8147275/94‑1 (MQ=60)
                                                                                            CCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCATTTTCTCGGTCG  >  SRR4302540.9497395/1‑94 (MQ=60)
                                                                                            |                                                                                             
TGTTAGCGTGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTTCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCATTTTCTCGGTCG  >  NC_000913/1758169‑1758354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑