Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,757,874 T→C H225R (CAT→CGT)  b1680 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,757,8740TC100.0% 623.4 / NA 190H225R (CAT→CGT) b1680hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (120/70);  total (120/70)

Only 100 of 192 total aligned reads displayed.

ATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTATGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACTTTT  >  NC_000913/1757784‑1757963
                                                                                          |                                                                                         
aTAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGc                                                                                         <  2:5493810/93‑1 (MQ=255)
 tAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGcc                                                                                        <  1:4031863/93‑1 (MQ=255)
 tAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGcc                                                                                        >  1:360686/1‑93 (MQ=255)
 tAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGcc                                                                                        >  2:2667355/1‑93 (MQ=255)
 tAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGcc                                                                                        >  2:2116311/1‑93 (MQ=255)
 tAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGcc                                                                                        <  1:2546159/93‑1 (MQ=255)
  aGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGccc                                                                                       >  2:4735631/1‑93 (MQ=255)
   gAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCg                                                                                      <  1:265323/93‑1 (MQ=255)
   gAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCg                                                                                      <  2:5218482/93‑1 (MQ=255)
   gAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCg                                                                                      >  1:2294104/1‑93 (MQ=255)
      ccGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGgag                                                                                   >  1:2476561/1‑93 (MQ=255)
       cGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGgaga                                                                                  >  2:5202546/1‑93 (MQ=255)
        ccccccTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGaa                                                                                 <  1:818655/92‑1 (MQ=255)
         cccccTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAac                                                                                >  1:3891921/1‑93 (MQ=255)
          ctccTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGTGGCCATACAGTTTACGCCCGGAGAaca                                                                               <  1:2728529/91‑1 (MQ=255)
          ccccTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAaca                                                                               >  1:2776524/1‑93 (MQ=255)
          ccccTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAaca                                                                               <  2:5365001/93‑1 (MQ=255)
          ccccTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAaca                                                                               >  1:4645407/1‑93 (MQ=255)
          ccccTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAaca                                                                               <  2:6287908/93‑1 (MQ=255)
           cccTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAacac                                                                              >  2:2464378/1‑93 (MQ=255)
           cccTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAacac                                                                              <  1:1926801/93‑1 (MQ=255)
            ccTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACg                                                                             <  2:1298817/93‑1 (MQ=255)
            ccTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACg                                                                             >  2:5974940/1‑93 (MQ=255)
             cTTCCCACGGCGTCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGt                                                                            <  1:4148705/93‑1 (MQ=255)
             cTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGt                                                                            <  1:4433566/93‑1 (MQ=255)
                 ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTaaaa                                                                        >  1:781407/1‑93 (MQ=255)
                 ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTaaaa                                                                        <  1:2873195/93‑1 (MQ=255)
                 ccACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTaaaa                                                                        >  1:3880363/1‑93 (MQ=255)
                  cACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAg                                                                       >  2:4844082/1‑93 (MQ=255)
                  cACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAg                                                                       <  1:4590511/93‑1 (MQ=255)
                    cggctgCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTc                                                                     <  1:5426453/93‑1 (MQ=255)
                     ggcggcATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCg                                                                    >  2:4590716/1‑93 (MQ=255)
                      gcggcATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGc                                                                   <  2:620202/93‑1 (MQ=255)
                       cggcATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCa                                                                  >  2:2152625/1‑93 (MQ=255)
                        ggcATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCaa                                                                 <  1:1046007/93‑1 (MQ=255)
                        ggcATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCaa                                                                 <  2:4315756/93‑1 (MQ=255)
                         gcATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAAt                                                                <  2:3669942/93‑1 (MQ=255)
                         gaaTCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAAt                                                                >  2:6210257/3‑93 (MQ=255)
                            tctcCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCa                                                             <  2:5927391/93‑1 (MQ=255)
                            tctcCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCa                                                             >  1:4017857/1‑93 (MQ=255)
                             ctcCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAg                                                            <  1:6208967/93‑1 (MQ=255)
                              tcCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAgc                                                           >  1:3703332/1‑93 (MQ=255)
                               ccTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAgcg                                                          <  2:349725/93‑1 (MQ=255)
                               ccTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAgcg                                                          >  2:404778/1‑93 (MQ=255)
                                 tGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGcc                                                        >  2:501882/1‑93 (MQ=255)
                                  gCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCt                                                       <  1:4793212/93‑1 (MQ=255)
                                   caacaaGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTg                                                      >  1:5505211/1‑93 (MQ=255)
                                    aacaaGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGa                                                     >  1:2496497/1‑93 (MQ=255)
                                      caaGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAAc                                                   >  1:6111373/1‑93 (MQ=255)
                                       aaGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACa                                                  <  2:2778107/93‑1 (MQ=255)
                                       aaGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACa                                                  >  2:4965680/1‑93 (MQ=255)
                                       aaGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACa                                                  >  1:1606683/1‑93 (MQ=255)
                                        aGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACAt                                                 >  2:2068752/1‑93 (MQ=255)
                                        aGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACAt                                                 <  1:5160383/93‑1 (MQ=255)
                                        aGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACAt                                                 >  1:2520629/1‑93 (MQ=255)
                                         ggCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATc                                                <  2:5989543/93‑1 (MQ=255)
                                          gCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATcc                                               >  1:3419989/1‑93 (MQ=255)
                                            ttcttTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCAc                                             <  2:369512/93‑1 (MQ=255)
                                            ttcttTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCAc                                             >  1:1030521/1‑93 (MQ=255)
                                                ttCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGga                                         <  2:1660119/93‑1 (MQ=255)
                                                ttCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGga                                         >  2:308816/1‑93 (MQ=255)
                                                 tCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGgat                                        <  2:3367212/93‑1 (MQ=255)
                                                     aTAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGatgatg                                    >  1:3297051/1‑93 (MQ=255)
                                                     aTAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGatgatg                                    >  1:233132/1‑93 (MQ=255)
                                                      tAAAGAATGCCGATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGc                                   <  1:1399948/93‑1 (MQ=255)
                                                      tAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGc                                   >  2:2226862/1‑93 (MQ=255)
                                                      tAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGc                                   >  2:1716935/1‑93 (MQ=255)
                                                       aaaGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCa                                  <  1:3763207/93‑1 (MQ=255)
                                                        aaGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCAt                                 >  1:6183013/1‑93 (MQ=255)
                                                        aaGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCAt                                 <  1:661875/93‑1 (MQ=255)
                                                         aGAATGCGAATTCCGGTGGGGACAGACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATc                                <  2:3823139/93‑1 (MQ=255)
                                                          gAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCa                               >  1:2304103/1‑93 (MQ=255)
                                                          gAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCa                               <  2:2977928/93‑1 (MQ=255)
                                                          gAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCa                               >  2:4926319/1‑93 (MQ=255)
                                                           aaTGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCAc                              >  2:3214031/1‑93 (MQ=255)
                                                           aaTGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCAc                              <  2:3936687/93‑1 (MQ=255)
                                                             tGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCg                            >  2:3888895/1‑93 (MQ=255)
                                                             tGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCg                            >  1:4058522/1‑93 (MQ=255)
                                                                cAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCt                         >  2:2326853/1‑93 (MQ=255)
                                                                  aTTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGa                       <  1:2025528/93‑1 (MQ=255)
                                                                   ttCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAg                      >  1:4316518/1‑93 (MQ=255)
                                                                    tCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAgc                     <  2:5431303/93‑1 (MQ=255)
                                                                       ggTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGcc                  >  1:3950167/1‑93 (MQ=255)
                                                                        gTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCa                 >  1:904417/1‑93 (MQ=255)
                                                                        gTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCa                 >  1:1259568/1‑93 (MQ=255)
                                                                          ggggCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCATCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATc               <  1:4086093/93‑1 (MQ=255)
                                                                          ggggCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATc               <  2:2325641/93‑1 (MQ=255)
                                                                           gggCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATcc              <  2:3135327/93‑1 (MQ=255)
                                                                           gggCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATcc              <  2:1351624/93‑1 (MQ=255)
                                                                            ggCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATcca             <  1:5183875/93‑1 (MQ=255)
                                                                               cATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATccacca          >  2:4676539/1‑93 (MQ=255)
                                                                                aTACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAg         >  2:3833891/1‑93 (MQ=255)
                                                                                aTACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAg         <  1:3829881/93‑1 (MQ=255)
                                                                                aTACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAg         >  1:2607894/1‑93 (MQ=255)
                                                                                 tACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGc        >  1:4494268/1‑93 (MQ=255)
                                                                                 tACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGc        >  1:3746798/1‑93 (MQ=255)
                                                                                 tACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGc        >  1:974534/1‑93 (MQ=255)
                                                                                      tttACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACttt   >  1:5941242/1‑93 (MQ=255)
                                                                                      tttACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACttt   <  2:3843708/93‑1 (MQ=255)
                                                                                       ttACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACtttt  >  1:5807881/1‑93 (MQ=255)
                                                                                          |                                                                                         
ATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTATGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACTTTT  >  NC_000913/1757784‑1757963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTATGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACTTTTGC  >  NC_000913/1757782‑1757965
                                                                                            |                                                                                           
TCATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACG                                                                                            >  SRR4302533.4302378/1‑94 (MQ=60)
TCATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACG                                                                                            <  SRR4302533.2581818/94‑1 (MQ=60)
TCATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACG                                                                                            <  SRR4302533.1356354/94‑1 (MQ=60)
 CATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGC                                                                                           <  SRR4302533.561552/94‑1 (MQ=60)
 CATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGC                                                                                           <  SRR4302533.3426467/94‑1 (MQ=60)
 CATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGC                                                                                           <  SRR4302533.133397/94‑1 (MQ=60)
  ATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCC                                                                                          <  SRR4302533.3740285/94‑1 (MQ=60)
  ATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCC                                                                                          >  SRR4302533.362880/1‑94 (MQ=60)
   TAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCC                                                                                         <  SRR4302533.2561825/94‑1 (MQ=60)
   TAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCC                                                                                         <  SRR4302533.4056407/94‑1 (MQ=60)
    AGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCG                                                                                        >  SRR4302533.2308306/1‑94 (MQ=60)
     GAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGG                                                                                       <  SRR4302533.266937/94‑1 (MQ=60)
       ACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAG                                                                                     >  SRR4302533.2491836/1‑94 (MQ=60)
         CGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAA                                                                                   >  SRR4302533.5977190/1‑94 (MQ=60)
          GCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAAC                                                                                  >  SRR4302533.3915675/1‑94 (MQ=60)
          GCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGATTACGCCCGGAGAAG                                                                                  >  SRR4302533.3915674/1‑94 (MQ=60)
          CCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAAC                                                                                  <  SRR4302533.823451/94‑1 (MQ=60)
           CCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACA                                                                                 >  SRR4302533.2793581/1‑94 (MQ=60)
           CCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACA                                                                                 >  SRR4302533.4673559/1‑94 (MQ=60)
            CTCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGTGGCCATACAGTTTACGCCCGGAGAACAC                                                                                <  SRR4302533.2745267/94‑1 (MQ=60)
             CCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACG                                                                               >  SRR4302533.3213890/1‑94 (MQ=60)
             CCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACG                                                                               <  SRR4302533.1938561/94‑1 (MQ=60)
               CTTCCCACGGCGTCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTA                                                                             <  SRR4302533.4173842/94‑1 (MQ=60)
               CTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTA                                                                             <  SRR4302533.4460371/94‑1 (MQ=60)
                 TCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAA                                                                           >  SRR4302533.2597873/1‑94 (MQ=60)
                  CCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAA                                                                          >  SRR4302533.786008/1‑94 (MQ=60)
                  CCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAA                                                                          >  SRR4302533.3904039/1‑94 (MQ=60)
                   CCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAG                                                                         <  SRR4302533.2890874/94‑1 (MQ=60)
                   CCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAG                                                                         <  SRR4302533.5447489/94‑1 (MQ=60)
                   CCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAG                                                                         >  SRR4302533.4605912/1‑94 (MQ=60)
                    CACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGT                                                                        <  SRR4302533.4618316/94‑1 (MQ=60)
                     ACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGGTTACGCCCGGAGAACACGTAAAAGTA                                                                       >  SRR4302533.3772754/1‑94 (MQ=60)
                      CGGCTGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCG                                                                      <  SRR4302533.5459133/94‑1 (MQ=60)
                         CGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAA                                                                   >  SRR4302533.2498641/1‑94 (MQ=60)
                          GGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAAT                                                                  <  SRR4302533.1735421/94‑1 (MQ=60)
                          GGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAAT                                                                  <  SRR4302533.1052155/94‑1 (MQ=60)
                          GGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAAT                                                                  <  SRR4302533.2535298/94‑1 (MQ=60)
                           GCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATC                                                                 >  SRR4302533.6163771/1‑94 (MQ=60)
                             ATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCA                                                               >  SRR4302533.4042319/1‑94 (MQ=60)
                             ATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCA                                                               >  SRR4302533.3390389/1‑94 (MQ=60)
                               CTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGC                                                             >  SRR4302533.3726078/1‑94 (MQ=60)
                               CTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGC                                                             <  SRR4302533.6246207/94‑1 (MQ=60)
                                TCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCG                                                            >  SRR4302533.3651005/1‑94 (MQ=60)
                                TCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCG                                                            >  SRR4302533.2634711/1‑94 (MQ=60)
                                TCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCG                                                            >  SRR4302533.2384696/1‑94 (MQ=60)
                                 CCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGC                                                           >  SRR4302533.2708081/1‑94 (MQ=60)
                                 CCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGC                                                           >  SRR4302533.2319157/1‑94 (MQ=60)
                                  CTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCC                                                          >  SRR4302533.3791420/1‑94 (MQ=60)
                                    GCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTG                                                        >  SRR4302533.5538428/1‑94 (MQ=60)
                                    GCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTG                                                        <  SRR4302533.4822252/94‑1 (MQ=60)
                                     CAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGA                                                       >  SRR4302533.4090521/1‑94 (MQ=60)
                                     CAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGA                                                       >  SRR4302533.4954625/1‑94 (MQ=60)
                                     CAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGA                                                       >  SRR4302533.2511897/1‑94 (MQ=60)
                                      AACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAA                                                      >  SRR4302533.4139553/1‑94 (MQ=60)
                                      AACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAA                                                      >  SRR4302533.4377692/1‑94 (MQ=60)
                                      AACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAA                                                      >  SRR4302533.1243889/1‑94 (MQ=60)
                                       ACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAAC                                                     >  SRR4302533.6148261/1‑94 (MQ=60)
                                        CAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACA                                                    >  SRR4302533.1616434/1‑94 (MQ=60)
                                         AAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACAT                                                   >  SRR4302533.2536159/1‑94 (MQ=60)
                                         AAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACAT                                                   >  SRR4302533.3459919/1‑94 (MQ=60)
                                         AAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACAT                                                   >  SRR4302533.2761028/1‑94 (MQ=60)
                                          AGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATC                                                  <  SRR4302533.5191527/94‑1 (MQ=60)
                                           GGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCC                                                 >  SRR4302533.3441121/1‑94 (MQ=60)
                                           GGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCC                                                 >  SRR4302533.4203229/1‑94 (MQ=60)
                                           GGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCA                                                 >  SRR4302533.4051957/1‑94 (MQ=60)
                                            GCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCA                                                >  SRR4302533.4076845/1‑94 (MQ=60)
                                            GCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCA                                                <  SRR4302533.287729/94‑1 (MQ=60)
                                             CTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCAC                                               >  SRR4302533.1036583/1‑94 (MQ=60)
                                               TCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCG                                             <  SRR4302533.896603/94‑1 (MQ=60)
                                                  TTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGAT                                          <  SRR4302533.545874/94‑1 (MQ=60)
                                                   TCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATG                                         <  SRR4302533.6287505/94‑1 (MQ=60)
                                                     ACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGAT                                       >  SRR4302533.3913844/1‑94 (MQ=60)
                                                      CATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATG                                      >  SRR4302533.234572/1‑94 (MQ=60)
                                                      CATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATG                                      >  SRR4302533.3317399/1‑94 (MQ=60)
                                                        TAAAGAATGCCGATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCA                                    <  SRR4302533.1408386/94‑1 (MQ=60)
                                                         AAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCAT                                   >  SRR4302533.6220138/1‑94 (MQ=60)
                                                         AAAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCAT                                   <  SRR4302533.3786274/94‑1 (MQ=60)
                                                          AAGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATC                                  <  SRR4302533.665780/94‑1 (MQ=60)
                                                           AGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCA                                 >  SRR4302533.591281/1‑94 (MQ=60)
                                                           AGAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCA                                 >  SRR4302533.2318374/1‑94 (MQ=60)
                                                            GAATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCAC                                >  SRR4302533.6245674/1‑94 (MQ=60)
                                                              ATGCCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCG                              >  SRR4302533.4083229/1‑94 (MQ=60)
                                                                 CCAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCT                           >  SRR4302533.2314836/1‑94 (MQ=60)
                                                                  CAATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTG                          >  SRR4302533.4766525/1‑94 (MQ=60)
                                                                   AATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGA                         >  SRR4302533.1943347/1‑94 (MQ=60)
                                                                    ATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAG                        >  SRR4302533.4342511/1‑94 (MQ=60)
                                                                    ATTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAG                        <  SRR4302533.2038033/94‑1 (MQ=60)
                                                                     TTCCGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGC                       >  SRR4302533.3919553/1‑94 (MQ=60)
                                                                        CGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCC                    >  SRR4302533.764607/1‑94 (MQ=60)
                                                                        CGGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCC                    >  SRR4302533.3974252/1‑94 (MQ=60)
                                                                         GGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCA                   >  SRR4302533.1273929/1‑94 (MQ=60)
                                                                         GGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCA                   >  SRR4302533.1267102/1‑94 (MQ=60)
                                                                         GGTGGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCA                   >  SRR4302533.909719/1‑94 (MQ=60)
                                                                            GGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCATCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCC                <  SRR4302533.4110966/94‑1 (MQ=60)
                                                                            GGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCC                >  SRR4302533.5781015/1‑94 (MQ=60)
                                                                            GGGGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCC                >  SRR4302533.4501160/1‑94 (MQ=60)
                                                                              GGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCAC              >  SRR4302533.2064473/1‑94 (MQ=60)
                                                                              GGCCATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCAC              <  SRR4302533.5215169/94‑1 (MQ=60)
                                                                                 CATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAG           >  SRR4302533.2623909/1‑94 (MQ=60)
                                                                                  ATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGC          >  SRR4302533.980254/1‑94 (MQ=60)
                                                                                  ATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGC          >  SRR4302533.4521484/1‑94 (MQ=60)
                                                                                  ATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGC          <  SRR4302533.3853294/94‑1 (MQ=60)
                                                                                  ATACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGC          >  SRR4302533.3769769/1‑94 (MQ=60)
                                                                                   TACAGTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCA         >  SRR4302533.1576232/1‑94 (MQ=60)
                                                                                       GTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACTTT     >  SRR4302533.5977170/1‑94 (MQ=60)
                                                                                       GTTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATAACCGCCTGAGCGCCATCCACCAGCACTTT     >  SRR4302533.4017269/1‑94 (MQ=60)
                                                                                        TTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACTTTT    >  SRR4302533.5842953/1‑94 (MQ=60)
                                                                                        TTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACTTTT    <  SRR4302533.5202627/94‑1 (MQ=60)
                                                                                        TTTACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACTTTT    <  SRR4302533.950333/94‑1 (MQ=60)
                                                                                          TACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACTTTTGC  <  SRR4302533.4602374/94‑1 (MQ=60)
                                                                                          TACGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACTTTTGC  >  SRR4302533.1900638/1‑94 (MQ=60)
                                                                                            |                                                                                           
TCATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCTTTCACATAAAGAATGCCAATTCCGGTGGGGCCATACAGTTTATGCCCGGAGAACACGTAAAAGTCGCAATCCAGCGCCTGAACATCCACCGGATGATGCATCACCGCCTGAGCGCCATCCACCAGCACTTTTGC  >  NC_000913/1757782‑1757965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑