Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,791,298 T→C T95T (ACA→ACG)  btuD ← ATP‑binding component of vitamin B12 transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,791,2980TC98.5% 621.7 / ‑1.4 198T95T (ACA→ACG) btuDATP‑binding component of vitamin B12 transport system
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base C (94/101);  total (95/103)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.04e-01

Only 100 of 206 total aligned reads displayed.

ATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCA  >  NC_000913/1791208‑1791387
                                                                                          |                                                                                         
 ttGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTc                                                                                        <  2:7672031/93‑1 (MQ=255)
   ggTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAg                                                                                      >  2:2128056/1‑93 (MQ=255)
      gCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTa                                                                                   <  2:9411862/93‑1 (MQ=255)
      gCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTa                                                                                   <  2:4470629/93‑1 (MQ=255)
      gCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTa                                                                                   <  2:5748217/93‑1 (MQ=255)
        tACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGt                                                                                 <  2:7518751/93‑1 (MQ=255)
        tACGTCCCAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGt                                                                                 <  2:3227943/93‑1 (MQ=255)
           gTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGcc                                                                              >  2:7821947/1‑93 (MQ=255)
            tCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCa                                                                             >  1:2421672/1‑93 (MQ=255)
            tCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCa                                                                             >  1:9046572/1‑93 (MQ=255)
            gccGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTTCTGATGCAGCGTCATGTAGTGCCa                                                                             <  2:7561165/92‑1 (MQ=255)
              cGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGCGCCAGa                                                                           <  2:6905587/93‑1 (MQ=255)
               gAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGAc                                                                          >  1:8793655/1‑93 (MQ=255)
                aGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACTCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGAcc                                                                         <  1:6979652/93‑1 (MQ=255)
                aGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGAcc                                                                         <  1:9957061/93‑1 (MQ=255)
                aGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGAcc                                                                         <  1:4057192/93‑1 (MQ=255)
                     gTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGCAGTGCCAGACCGGCGt                                                                    >  2:4773661/1‑93 (MQ=255)
                        atcaAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGc                                                                 <  2:6603352/93‑1 (MQ=255)
                        atcaAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGc                                                                 <  1:7386899/93‑1 (MQ=255)
                        atcaAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGc                                                                 >  2:9611265/1‑93 (MQ=255)
                         tcaAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCa                                                                >  2:1411719/1‑93 (MQ=255)
                            agagCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAAc                                                             <  1:565670/93‑1 (MQ=255)
                            agagCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAAc                                                             >  2:4395214/1‑93 (MQ=255)
                            agagCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAAc                                                             <  2:967247/93‑1 (MQ=255)
                             gagCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTCTCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAAcg                                                            <  2:6005355/93‑1 (MQ=255)
                             gagCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAAcg                                                            >  2:6486466/1‑93 (MQ=255)
                              agCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAAcgg                                                           <  2:1140438/93‑1 (MQ=255)
                              agCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAAcgg                                                           <  2:5857126/93‑1 (MQ=255)
                               gCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAAcggc                                                          >  1:4863774/1‑93 (MQ=255)
                                ccAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAAcggcg                                                         >  2:7252787/1‑93 (MQ=255)
                                ccAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAAcggcg                                                         <  1:9893868/93‑1 (MQ=255)
                                   gcgcCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGgcggcg                                                      <  2:8861151/93‑1 (MQ=255)
                                   gcgcCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGgcggcg                                                      >  1:5209093/1‑93 (MQ=255)
                                   gcgcCCCTGCGACATCATTCAGTAGTTCGGGACGCGTTTTATCGTGCTGATGTAGCGTCAGGGAGCGCCAGACCGGCGTTGCAAAgggcgggt                                                      >  2:5044637/1‑91 (MQ=255)
                                    cgcCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGt                                                     >  1:6234249/1‑93 (MQ=255)
                                      ccccTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTct                                                   >  1:5152798/1‑93 (MQ=255)
                                        ccTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTctgc                                                 <  1:7248882/93‑1 (MQ=255)
                                        ccTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTctgc                                                 <  2:2427666/93‑1 (MQ=255)
                                         cTGCGACATCATTCAGTAGTTCGGAACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGCTGCAAACGGCGGCGTctgcg                                                >  2:280057/1‑92 (MQ=255)
                                          tGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTctgctg                                               >  2:5386178/1‑93 (MQ=255)
                                          tGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTctgctg                                               >  1:2394016/1‑93 (MQ=255)
                                            cGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCtgtt                                             <  1:9408201/93‑1 (MQ=255)
                                             gACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCtgttg                                            >  1:6675734/1‑93 (MQ=255)
                                              gcaccatTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGTTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCtgttgt                                           <  2:1838047/89‑1 (MQ=255)
                                              aCATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCtgttgt                                           >  2:1495275/1‑93 (MQ=255)
                                              aCATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCtgttgt                                           >  1:1922110/1‑93 (MQ=255)
                                               catcatTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTtgtg                                          >  1:2272596/1‑93 (MQ=255)
                                               catcatTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTtgtg                                          <  2:6986815/93‑1 (MQ=255)
                                                 tcatTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGaa                                        >  1:5395980/1‑93 (MQ=255)
                                                  catTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGaaa                                       >  2:5220129/1‑93 (MQ=255)
                                                   atTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAg                                      <  2:8445958/93‑1 (MQ=255)
                                                    ttCAGTAGTTCGGTACGCGTTTTATCGTGGTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGa                                     <  1:1150963/93‑1 (MQ=255)
                                                     tCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGAt                                    <  2:2569713/93‑1 (MQ=255)
                                                     tCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGGGAAAGAt                                    >  2:803486/1‑93 (MQ=255)
                                                      cAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATa                                   >  1:9229555/1‑93 (MQ=255)
                                                      cAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATa                                   <  2:9114260/93‑1 (MQ=255)
                                                      cAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGCCTGCTGTTGTGAAAGATa                                   >  1:3389238/1‑93 (MQ=255)
                                                       agtagtTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAg                                  <  1:3014936/93‑1 (MQ=255)
                                                       agtagtTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAg                                  <  1:4131272/93‑1 (MQ=255)
                                                        gtagtTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGTCGTCTGCTGTTGTGTACGATAgg                                 >  2:5602669/1‑93 (MQ=255)
                                                        gtagtTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAgg                                 <  1:9414502/93‑1 (MQ=255)
                                                        gtagtTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAgg                                 >  1:5588537/1‑93 (MQ=255)
                                                        gtagtTCGGTACGCGTTTTATCGAGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAgg                                 <  2:5503161/93‑1 (MQ=255)
                                                        gtagtTCGCTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAgg                                 <  2:3369694/93‑1 (MQ=255)
                                                         tagtTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGgc                                >  2:3659153/1‑93 (MQ=255)
                                                         tagtTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGgc                                <  1:3035790/93‑1 (MQ=255)
                                                          agtTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGgcg                               <  2:3445646/93‑1 (MQ=255)
                                                          agtTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGgcg                               <  1:2879248/93‑1 (MQ=255)
                                                             tCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGa                            >  2:913263/1‑93 (MQ=255)
                                                             tCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGa                            <  1:637872/93‑1 (MQ=255)
                                                                gTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGc                         <  1:5864338/93‑1 (MQ=255)
                                                                  aCGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAg                       <  1:8305810/93‑1 (MQ=255)
                                                                   cgcgTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAgc                      <  1:7516767/93‑1 (MQ=255)
                                                                    gcgTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAgcg                     >  1:1119373/1‑93 (MQ=255)
                                                                    gcgTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCCGCGGCTGCTGGTGGGAGAGAGAGGCGCGGTGCAgcg                     >  1:4930538/1‑93 (MQ=255)
                                                                     cgTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAgcgc                    <  1:7903645/93‑1 (MQ=255)
                                                                      gTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGcgcg                   >  1:4245976/1‑93 (MQ=255)
                                                                      gTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGcgcg                   >  2:1065486/1‑93 (MQ=255)
                                                                        tttATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAg                 <  1:2904844/93‑1 (MQ=255)
                                                                         ttATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGt                >  1:6175530/1‑93 (MQ=255)
                                                                          tATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGtt               <  1:4829900/93‑1 (MQ=255)
                                                                           aTCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGttt              <  1:5385577/93‑1 (MQ=255)
                                                                            tCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGtttt             <  2:6318732/93‑1 (MQ=255)
                                                                            tCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGtttt             >  1:1780425/1‑93 (MQ=255)
                                                                             cGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTttg            <  2:8896822/93‑1 (MQ=255)
                                                                             cGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTttg            <  1:2694041/93‑1 (MQ=255)
                                                                             cGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTttg            <  1:3706528/93‑1 (MQ=255)
                                                                              gTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTttgt           <  1:2252241/93‑1 (MQ=255)
                                                                               tGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGGTGCAAACGGCCGGGTCTGCTGGTGTGAAAGATAGGCGCGATGCAGCGCGAGCTttgtt          >  2:1579203/1‑93 (MQ=255)
                                                                                gCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTttgttg         <  1:2392760/93‑1 (MQ=255)
                                                                                gCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTttgttg         >  1:8450825/1‑93 (MQ=255)
                                                                                gCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTttgttg         >  1:4320299/1‑93 (MQ=255)
                                                                                  tGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGGTTTGGTGCg       >  2:5260947/1‑93 (MQ=255)
                                                                                  tGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGAAGCAGCGCGAGTTTTGTTGCg       >  1:1337111/1‑93 (MQ=255)
                                                                                    aTGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGa     <  1:807304/93‑1 (MQ=255)
                                                                                     tGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGAc    <  2:2854854/93‑1 (MQ=255)
                                                                                      gCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGAcc   >  2:3167116/1‑93 (MQ=255)
                                                                                      gCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGAcc   >  2:3771290/1‑93 (MQ=255)
                                                                                       cAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCa  <  1:4958152/93‑1 (MQ=255)
                                                                                       cAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCa  <  1:652240/93‑1 (MQ=255)
                                                                                          |                                                                                         
ATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCA  >  NC_000913/1791208‑1791387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGC  >  NC_000913/1791206‑1791390
                                                                                            |                                                                                            
TGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCG                                                                                             <  SRR4302540.317603/94‑1 (MQ=60)
TGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCG                                                                                             >  SRR4302540.5698478/1‑94 (MQ=60)
  ATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTC                                                                                           >  SRR4302540.4729703/1‑94 (MQ=60)
     GGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGG                                                                                        >  SRR4302540.9837862/1‑94 (MQ=60)
        GCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAG                                                                                     <  SRR4302540.2277209/94‑1 (MQ=60)
          TACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTG                                                                                   <  SRR4302540.7820898/94‑1 (MQ=60)
             GTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCA                                                                                >  SRR4302540.9155168/1‑94 (MQ=60)
             GTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCA                                                                                >  SRR4302540.2447767/1‑94 (MQ=60)
              TCCGAGTTTGTCACCAAGAGCCAGCCCCCCTGCGACATCATTCAGTAGTTCGGTACTCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAG                                                                               <  SRR4302540.3195592/94‑1 (MQ=60)
                CGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGAC                                                                             >  SRR4302540.8895363/1‑94 (MQ=60)
                 GAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACC                                                                            <  SRR4302540.2759820/94‑1 (MQ=60)
                 GAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACC                                                                            <  SRR4302540.5234451/94‑1 (MQ=60)
                  AGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACTCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCG                                                                           <  SRR4302540.7057188/94‑1 (MQ=60)
                  AGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCG                                                                           <  SRR4302540.4100933/94‑1 (MQ=60)
                  AGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCG                                                                           <  SRR4302540.4100935/94‑1 (MQ=60)
                  AGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCG                                                                           <  SRR4302540.10073272/94‑1 (MQ=60)
                   GTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGG                                                                          >  SRR4302540.7019252/1‑94 (MQ=60)
                    TTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGC                                                                         >  SRR4302540.2926573/1‑94 (MQ=60)
                      TGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGT                                                                       >  SRR4302540.1593522/1‑94 (MQ=60)
                        TCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTG                                                                     <  SRR4302540.9233319/94‑1 (MQ=60)
                         CATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGC                                                                    <  SRR4302540.7566179/94‑1 (MQ=60)
                          ATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCA                                                                   <  SRR4302540.7469204/94‑1 (MQ=60)
                          ATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCA                                                                   <  SRR4302540.7469206/94‑1 (MQ=60)
                             AAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAAC                                                                >  SRR4302540.121420/1‑94 (MQ=60)
                             AAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAAC                                                                <  SRR4302540.8416044/94‑1 (MQ=60)
                              AGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACG                                                               <  SRR4302540.571819/94‑1 (MQ=60)
                              AGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACG                                                               >  SRR4302540.4352783/1‑94 (MQ=60)
                                AGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGC                                                             <  SRR4302540.5682866/94‑1 (MQ=60)
                                AGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGC                                                             >  SRR4302540.4915938/1‑94 (MQ=60)
                                  CCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGG                                                           <  SRR4302540.10009408/94‑1 (MQ=60)
                                    AGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCG                                                         >  SRR4302540.5265842/1‑94 (MQ=60)
                                    AGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCG                                                         >  SRR4302540.5094941/1‑94 (MQ=60)
                                    AGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCG                                                         >  SRR4302540.3652566/1‑94 (MQ=60)
                                     GCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGT                                                        >  SRR4302540.6303585/1‑94 (MQ=60)
                                     GCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGT                                                        >  SRR4302540.6303586/1‑94 (MQ=60)
                                      CGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTC                                                       <  SRR4302540.6687913/94‑1 (MQ=60)
                                       GCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCT                                                      >  SRR4302540.4126673/1‑94 (MQ=60)
                                       GCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCT                                                      >  SRR4302540.5208711/1‑94 (MQ=60)
                                        CCCATGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTG                                                     <  SRR4302540.3961153/94‑1 (MQ=60)
                                          CCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCT                                                   <  SRR4302540.6378593/94‑1 (MQ=60)
                                          CCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCT                                                   <  SRR4302540.884973/94‑1 (MQ=60)
                                          CCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCT                                                   <  SRR4302540.7329765/94‑1 (MQ=60)
                                           CTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTG                                                  >  SRR4302540.2419810/1‑94 (MQ=60)
                                              CGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTG                                               >  SRR4302540.10027992/1‑94 (MQ=60)
                                              CGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTG                                               <  SRR4302540.9520084/94‑1 (MQ=60)
                                              CGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTG                                               >  SRR4302540.6749480/1‑94 (MQ=60)
                                              CGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTG                                               >  SRR4302540.1886099/1‑94 (MQ=60)
                                               GACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGT                                              >  SRR4302540.1943070/1‑94 (MQ=60)
                                               GACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGT                                              <  SRR4302540.5453615/94‑1 (MQ=60)
                                               GACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGT                                              <  SRR4302540.8130990/94‑1 (MQ=60)
                                                ACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTG                                             >  SRR4302540.2296990/1‑94 (MQ=60)
                                                  ATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAA                                           >  SRR4302540.5455309/1‑94 (MQ=60)
                                                  AACATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGAGCCAGACCGGCGGTGCAAACGGCGGCGGCTGCTGTTGTGAA                                           >  SRR4302540.9095339/1‑94 (MQ=60)
                                                     ATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGA                                        >  SRR4302540.5363997/1‑94 (MQ=60)
                                                      TTCAGTAGTTCGGTACGCGTTTTATCGTGGTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGAT                                       <  SRR4302540.1162958/94‑1 (MQ=60)
                                                       TCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATA                                      >  SRR4302540.9340194/1‑94 (MQ=60)
                                                       TCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGCCTGCTGTTGTGAAAGATA                                      >  SRR4302540.3426217/1‑94 (MQ=60)
                                                         AGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGG                                    <  SRR4302540.4175950/94‑1 (MQ=60)
                                                         AGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGG                                    >  SRR4302540.5650043/1‑94 (MQ=60)
                                                         AGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGG                                    <  SRR4302540.3048087/94‑1 (MQ=60)
                                                          GTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGC                                   <  SRR4302540.9526428/94‑1 (MQ=60)
                                                           TAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCG                                  <  SRR4302540.3069108/94‑1 (MQ=60)
                                                           GAGTTCGGTGCGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCG                                  <  SRR4302540.1144246/94‑1 (MQ=60)
                                                            AGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGC                                 >  SRR4302540.5747761/1‑94 (MQ=60)
                                                            AGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGC                                 <  SRR4302540.2910564/94‑1 (MQ=60)
                                                               TCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGAT                              <  SRR4302540.644657/94‑1 (MQ=60)
                                                                  GTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCA                           >  SRR4302540.2435426/1‑94 (MQ=60)
                                                                  GTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCA                           <  SRR4302540.5929174/94‑1 (MQ=60)
                                                                  GTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCA                           >  SRR4302540.4982045/1‑94 (MQ=60)
                                                                   TACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAG                          <  SRR4302540.5469792/94‑1 (MQ=60)
                                                                   TACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAG                          >  SRR4302540.9525403/1‑94 (MQ=60)
                                                                   TACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAG                          <  SRR4302540.2317393/94‑1 (MQ=60)
                                                                    ACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGC                         <  SRR4302540.8402253/94‑1 (MQ=60)
                                                                     CGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCG                        >  SRR4302540.1130984/1‑94 (MQ=60)
                                                                     CGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCG                        <  SRR4302540.7600810/94‑1 (MQ=60)
                                                                     CGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCCGCGGCTGCTGGTGGGAGAGAGAGGCGCGGTGCAGCG                        >  SRR4302540.4983690/1‑94 (MQ=60)
                                                                       CGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCG                      >  SRR4302540.4292175/1‑94 (MQ=60)
                                                                       CGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCG                      <  SRR4302540.7995822/94‑1 (MQ=60)
                                                                        GTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTGGCAAACGGCGGCGTCTGCTGGTGTGAAAGATAGGCGCGATGCAGCGCGA                     >  SRR4302540.2968256/1‑94 (MQ=60)
                                                                        GTGTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGA                     <  SRR4302540.7284920/94‑1 (MQ=60)
                                                                         TTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCCGCGCGAG                    >  SRR4302540.2676717/1‑94 (MQ=60)
                                                                         TTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAG                    >  SRR4302540.4191439/1‑94 (MQ=60)
                                                                          TTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGT                   <  SRR4302540.2936553/94‑1 (MQ=60)
                                                                          TTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGT                   >  SRR4302540.6113841/1‑94 (MQ=60)
                                                                          TTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGT                   >  SRR4302540.6244330/1‑94 (MQ=60)
                                                                          TTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGA                   <  SRR4302540.2053163/94‑1 (MQ=60)
                                                                            TATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTT                 <  SRR4302540.4913824/94‑1 (MQ=60)
                                                                            TATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTT                 <  SRR4302540.4881628/94‑1 (MQ=60)
                                                                             ATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTT                <  SRR4302540.5444810/94‑1 (MQ=60)
                                                                             ATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTT                >  SRR4302540.1799754/1‑94 (MQ=60)
                                                                               CGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGT              <  SRR4302540.2723128/94‑1 (MQ=60)
                                                                               CGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGT              <  SRR4302540.3746984/94‑1 (MQ=60)
                                                                                GTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTT             <  SRR4302540.2276415/94‑1 (MQ=60)
                                                                                 TGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGTCGCGATGCAGCGCGAGTTTTGTTT            >  SRR4302540.9804053/1‑94 (MQ=60)
                                                                                 TGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTG            >  SRR4302540.4367333/1‑94 (MQ=60)
                                                                                 TGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTG            >  SRR4302540.8548936/1‑94 (MQ=60)
                                                                                  GCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGC           <  SRR4302540.2418540/94‑1 (MQ=60)
                                                                                  GCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGC           <  SRR4302540.2418541/94‑1 (MQ=60)
                                                                                  GCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGC           >  SRR4302540.65512/1‑94 (MQ=60)
                                                                                   CTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGAAGCAGCGCGAGTTTTGTTGCG          >  SRR4302540.1351111/1‑94 (MQ=60)
                                                                                     GATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGA        >  SRR4302540.3052553/1‑94 (MQ=60)
                                                                                     GATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGA        >  SRR4302540.936093/1‑94 (MQ=60)
                                                                                      ATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGAC       <  SRR4302540.815883/94‑1 (MQ=60)
                                                                                      ATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGAC       >  SRR4302540.6325675/1‑94 (MQ=60)
                                                                                       TGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACC      <  SRR4302540.8299246/94‑1 (MQ=60)
                                                                                         CAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCAT    <  SRR4302540.659178/94‑1 (MQ=60)
                                                                                         CAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCAT    <  SRR4302540.5011742/94‑1 (MQ=60)
                                                                                         CAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCAT    <  SRR4302540.5011741/94‑1 (MQ=60)
                                                                                          AGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATG   <  SRR4302540.9519104/94‑1 (MQ=60)
                                                                                          AGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATG   >  SRR4302540.1095768/1‑94 (MQ=60)
                                                                                          AGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATG   <  SRR4302540.9692476/94‑1 (MQ=60)
                                                                                          AGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATG   <  SRR4302540.7184838/94‑1 (MQ=60)
                                                                                          AGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATG   <  SRR4302540.8764329/94‑1 (MQ=60)
                                                                                           GCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGC  <  SRR4302540.3806126/94‑1 (MQ=60)
                                                                                            |                                                                                            
TGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGC  >  NC_000913/1791206‑1791390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑