Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,822,045 G→A L169L (CTG→CTA)  b1741 → putative excinuclease subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,822,0450GA91.5% 702.4 / 29.1 237L169L (CTG→CTA) b1741putative excinuclease subunit
Reads supporting (aligned to +/- strand):  ref base G (13/7);  new base A (116/99);  total (130/107)
Fisher's exact test for biased strand distribution p-value = 4.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.11e-01

Only 100 of 251 total aligned reads displayed.

CCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCA  >  NC_000913/1821954‑1822134
                                                                                           |                                                                                         
 ccccAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCctact                                                                                         >  1:872271/1‑93 (MQ=255)
 ccccAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCctact                                                                                         <  2:1068252/93‑1 (MQ=255)
 ccccAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCctact                                                                                         >  1:1004240/1‑93 (MQ=255)
  cccAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCctactg                                                                                        >  2:6455763/1‑93 (MQ=255)
  cccAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCctactg                                                                                        >  1:298311/1‑93 (MQ=255)
  cccAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCctactg                                                                                        >  1:2243664/1‑93 (MQ=255)
  cccAACCTGTTTGGCCTGTTTACCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCctgctg                                                                                        >  2:4230947/1‑93 (MQ=255)
   ccAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTgg                                                                                       >  1:4743607/1‑93 (MQ=255)
    cAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTggg                                                                                      >  2:2352013/1‑93 (MQ=255)
     aaCCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGa                                                                                     >  1:7927687/1‑93 (MQ=255)
     aaCCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGa                                                                                     <  2:2531237/93‑1 (MQ=255)
           tttGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGaa                                                                               >  2:3178436/1‑93 (MQ=255)
            ttGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAAc                                                                              <  1:2049171/93‑1 (MQ=255)
            ttGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAAc                                                                              <  1:8321007/93‑1 (MQ=255)
            ttGACCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAAc                                                                              <  1:9396741/93‑1 (MQ=255)
             tGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAAcc                                                                             <  2:5690669/93‑1 (MQ=255)
              gtccTTTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGTCCTTCTGGGACTGGTACCg                                                                            >  2:8977544/3‑93 (MQ=255)
               gCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGGCTGGAACCGt                                                                           >  2:3124441/1‑93 (MQ=255)
               gCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGt                                                                           <  1:1445273/93‑1 (MQ=255)
                ccTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGtt                                                                          <  1:9163661/93‑1 (MQ=255)
                 cTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTa                                                                         <  2:8518731/93‑1 (MQ=255)
                   gTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGGCTGGAACCGTTAAg                                                                       >  2:5646207/1‑93 (MQ=255)
                    tttGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGt                                                                      >  2:8855298/1‑93 (MQ=255)
                    tttGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGt                                                                      >  2:6970132/1‑93 (MQ=255)
                    tttGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGt                                                                      <  2:3428319/93‑1 (MQ=255)
                    tttGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAAC‑AAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTc                                                                     >  2:1014294/1‑93 (MQ=255)
                          aaTAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGt                                                                >  1:1085765/1‑93 (MQ=255)
                          aaTAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGt                                                                >  1:1364530/1‑93 (MQ=255)
                            tAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCg                                                              >  2:7110076/1‑93 (MQ=255)
                              ggCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTg                                                            <  2:8439631/93‑1 (MQ=255)
                               gcgcgcAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGc                                                           >  2:576331/1‑93 (MQ=255)
                               gcgcgcAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGc                                                           <  2:7542141/93‑1 (MQ=255)
                               gcgcgcAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGc                                                           <  2:4459697/93‑1 (MQ=255)
                                cgcgcAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCa                                                          >  1:9263649/1‑93 (MQ=255)
                                 gcgcAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCAt                                                         <  2:4653057/93‑1 (MQ=255)
                                   gcAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGt                                                       <  1:8550176/93‑1 (MQ=255)
                                   gcAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTCGAACCGTTAAGTCGCGGTCGTGCATGt                                                       >  1:1510064/1‑93 (MQ=255)
                                    cAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGtt                                                      >  2:877204/1‑93 (MQ=255)
                                     aGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGttt                                                     <  2:9344814/93‑1 (MQ=255)
                                      gatttGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGtttt                                                    >  1:3743435/3‑93 (MQ=255)
                                       cTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTc                                                   <  1:4621005/93‑1 (MQ=255)
                                        tttGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCg                                                  >  1:6976830/1‑93 (MQ=255)
                                        tttGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCg                                                  >  1:7287350/1‑93 (MQ=255)
                                           gCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTc                                               >  1:2335001/1‑93 (MQ=255)
                                           gCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTc                                               >  2:543105/1‑93 (MQ=255)
                                           gCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTc                                               >  1:3410061/1‑93 (MQ=255)
                                           gCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTc                                               <  2:4053466/93‑1 (MQ=255)
                                             aaGCATTGCAGACCATCGCTGATGAACAAAAACTTTGGTATGGCCTACTGGGACTGGAACGGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAg                                             >  1:6117509/1‑93 (MQ=255)
                                                cATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGGCTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAgcgc                                          <  1:3039549/93‑1 (MQ=255)
                                                cATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAgcgc                                          >  2:367032/1‑93 (MQ=255)
                                                cATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAgcgc                                          <  1:2697309/93‑1 (MQ=255)
                                                 aTTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCt                                         >  1:5962292/1‑93 (MQ=255)
                                                    gCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTaaa                                      >  1:5018034/1‑93 (MQ=255)
                                                     cAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTaaaa                                     <  1:3233859/93‑1 (MQ=255)
                                                      aGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAAc                                    >  1:7611179/1‑93 (MQ=255)
                                                      aGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAAc                                    >  1:1816339/1‑93 (MQ=255)
                                                      aGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAAc                                    >  2:7709049/1‑93 (MQ=255)
                                                       gACCATCGCTGATGAAGAAAAACTTTGTTATGGCCTGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACg                                   >  2:6706443/1‑93 (MQ=255)
                                                       gACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACg                                   >  1:9101189/1‑93 (MQ=255)
                                                       gACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACg                                   <  2:138012/93‑1 (MQ=255)
                                                       gACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACg                                   >  2:1701923/1‑93 (MQ=255)
                                                       gACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACg                                   >  1:1273592/1‑93 (MQ=255)
                                                       gACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGGGCATGTTTTCGTCCACCGCAAAAAct                                   >  1:4362789/1‑92 (MQ=255)
                                                         ccATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGtt                                 <  1:9864920/93‑1 (MQ=255)
                                                         ccATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGtt                                 >  1:7468135/1‑93 (MQ=255)
                                                         ccATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGtt                                 >  1:6078777/1‑93 (MQ=255)
                                                            tCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTgcg                              >  1:6991553/1‑93 (MQ=255)
                                                             cGCTGATGAACAAAAACTTTGTTATGGCCTGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTgcgc                             <  2:8037404/93‑1 (MQ=255)
                                                              gCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGcc                            >  2:2092711/1‑93 (MQ=255)
                                                                tgatgaACAAAAACTTTGTTATGGCCTACTGGGGCTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCgg                          >  2:7450407/1‑93 (MQ=255)
                                                                tgatgaACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCgg                          <  2:9563419/93‑1 (MQ=255)
                                                                 tttgaACAAATACTTTGTTTTGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTATCGTTTAGCGCTTATACGTTGCGCCTGa                         >  1:8947394/3‑93 (MQ=255)
                                                                 gatgaACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGa                         >  1:2739817/1‑93 (MQ=255)
                                                                  atgaACAAAAACTTTGTTATGGCCTACTGGGGCTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAg                        >  1:28032/1‑93 (MQ=255)
                                                                  atgaACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAg                        >  2:2596210/1‑93 (MQ=255)
                                                                  atgaACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAg                        <  2:9200064/93‑1 (MQ=255)
                                                                   tgaACAAAAACTTTGTTATGGCCTGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGc                       >  2:8913901/1‑93 (MQ=255)
                                                                   tgaACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGc                       >  2:6153785/1‑93 (MQ=255)
                                                                    gaACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCa                      >  2:4843008/1‑93 (MQ=255)
                                                                       cAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCAtgc                   <  1:3646762/93‑1 (MQ=255)
                                                                         aaaaCTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCAtgctg                 >  2:7835348/1‑93 (MQ=255)
                                                                         aaaaCTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCAtgctg                 >  1:5869004/1‑93 (MQ=255)
                                                                           aaCTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCg               >  1:5377485/1‑93 (MQ=255)
                                                                            aCTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCgg              >  2:1961545/1‑93 (MQ=255)
                                                                             cTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGt             <  1:7739016/93‑1 (MQ=255)
                                                                               ttGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTaa           <  2:5034952/93‑1 (MQ=255)
                                                                                 gTTATGGCCTGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAag         >  1:8159591/1‑93 (MQ=255)
                                                                                 gTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAag         <  1:9543197/93‑1 (MQ=255)
                                                                                 gTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAag         >  1:4229521/1‑93 (MQ=255)
                                                                                  ttATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCTCCGGAGCATGCTGCGGTAAaga        <  1:6656066/93‑1 (MQ=255)
                                                                                  ttATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAaga        <  1:2643553/93‑1 (MQ=255)
                                                                                  ttATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAaga        <  1:6608612/93‑1 (MQ=255)
                                                                                   tATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAagag       >  2:1197057/1‑93 (MQ=255)
                                                                                    aTGGCCTGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAagaga      >  2:6509119/1‑93 (MQ=255)
                                                                                      ggCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGc    >  2:2367125/1‑93 (MQ=255)
                                                                                       gCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGcc   <  1:6283152/93‑1 (MQ=255)
                                                                                       gCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGcc   >  2:2212342/1‑93 (MQ=255)
                                                                                        ccTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCa  >  2:8107616/1‑93 (MQ=255)
                                                                                        ccTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCa  >  1:9955745/1‑93 (MQ=255)
                                                                                        ccTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCCCCGGAGCATGCTGCGGTAAAGAGAGCCa  <  1:6068335/93‑1 (MQ=255)
                                                                                           |                                                                                         
CCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCA  >  NC_000913/1821954‑1822134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAG  >  NC_000913/1821953‑1822138
                                                                                            |                                                                                             
GCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTAC                                                                                              <  SRR4302540.4437085/94‑1 (MQ=60)
GCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTAC                                                                                              >  SRR4302540.2545105/1‑94 (MQ=60)
 CCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACT                                                                                             >  SRR4302540.1014432/1‑94 (MQ=60)
 CCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACT                                                                                             >  SRR4302540.1014433/1‑94 (MQ=60)
 CCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACT                                                                                             >  SRR4302540.881524/1‑94 (MQ=60)
 CCCCCAACCTGTTTGGCCTGTGTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTGCT                                                                                             <  SRR4302540.1229714/94‑1 (MQ=60)
  CCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTG                                                                                            >  SRR4302540.301262/1‑94 (MQ=60)
  CCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTG                                                                                            >  SRR4302540.598507/1‑94 (MQ=60)
  CCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTG                                                                                            >  SRR4302540.2267778/1‑94 (MQ=60)
   CCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGG                                                                                           >  SRR4302540.4794229/1‑94 (MQ=60)
   CCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGG                                                                                           <  SRR4302540.2187254/94‑1 (MQ=60)
    CCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGG                                                                                          >  SRR4302540.4820308/1‑94 (MQ=60)
    CCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGG                                                                                          <  SRR4302540.831988/94‑1 (MQ=60)
     CAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGA                                                                                         >  SRR4302540.8020082/1‑94 (MQ=60)
      AACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGAC                                                                                        >  SRR4302540.7079602/1‑94 (MQ=60)
        CCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTG                                                                                      <  SRR4302540.7300534/94‑1 (MQ=60)
        CCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTG                                                                                      >  SRR4302540.7396589/1‑94 (MQ=60)
        CCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTG                                                                                      <  SRR4302540.6584737/94‑1 (MQ=60)
         CTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTGCTGGGACTGG                                                                                     >  SRR4302540.6932566/1‑94 (MQ=60)
         CTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGG                                                                                     >  SRR4302540.932010/1‑94 (MQ=60)
           GTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAA                                                                                   >  SRR4302540.4675555/1‑94 (MQ=60)
           GTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAA                                                                                   <  SRR4302540.9547622/94‑1 (MQ=60)
             TTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACC                                                                                 <  SRR4302540.2071232/94‑1 (MQ=60)
             TTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACC                                                                                 <  SRR4302540.182315/94‑1 (MQ=60)
             TTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACC                                                                                 <  SRR4302540.8417595/94‑1 (MQ=60)
             TTGACCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACC                                                                                 <  SRR4302540.9508550/94‑1 (MQ=60)
                GCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTT                                                                              <  SRR4302540.1460985/94‑1 (MQ=60)
                GCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTT                                                                              <  SRR4302540.1460984/94‑1 (MQ=60)
                 CCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTA                                                                             <  SRR4302540.9273626/94‑1 (MQ=60)
                 CCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTA                                                                             >  SRR4302540.457739/1‑94 (MQ=60)
                 CCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTA                                                                             <  SRR4302540.7938450/94‑1 (MQ=60)
                  CTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGGCTGGAACCGTTAA                                                                            >  SRR4302540.7068011/1‑94 (MQ=60)
                  CTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAA                                                                            >  SRR4302540.9269645/1‑94 (MQ=60)
                  CTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAA                                                                            >  SRR4302540.4207535/1‑94 (MQ=60)
                      TTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCG                                                                        >  SRR4302540.9496858/1‑94 (MQ=60)
                      TTGCCAATAGGCGCGCAACTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCG                                                                        <  SRR4302540.9086051/94‑1 (MQ=60)
                       TGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGC                                                                       >  SRR4302540.8763766/1‑94 (MQ=60)
                        GCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCG                                                                      >  SRR4302540.6691332/1‑94 (MQ=60)
                         CGAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGG                                                                     <  SRR4302540.7894765/94‑1 (MQ=60)
                          CAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGT                                                                    >  SRR4302540.1096893/1‑94 (MQ=60)
                          CAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGT                                                                    >  SRR4302540.1378948/1‑94 (MQ=60)
                          CAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGT                                                                    >  SRR4302540.2953333/1‑94 (MQ=60)
                          CAATAGGCGCGCAGCGTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGT                                                                    <  SRR4302540.2679392/94‑1 (MQ=60)
                            ATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCG                                                                  <  SRR4302540.6163620/94‑1 (MQ=60)
                            ATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCG                                                                  >  SRR4302540.761367/1‑94 (MQ=60)
                              AGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTG                                                                >  SRR4302540.9362011/1‑94 (MQ=60)
                               GGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGC                                                               <  SRR4302540.8904679/94‑1 (MQ=60)
                                GCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCA                                                              >  SRR4302540.3897150/1‑94 (MQ=60)
                                GCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCA                                                              >  SRR4302540.9374462/1‑94 (MQ=60)
                                GCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCA                                                              >  SRR4302540.3337771/1‑94 (MQ=60)
                                GCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCA                                                              <  SRR4302540.5270391/94‑1 (MQ=60)
                                   CGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGT                                                           >  SRR4302540.2750233/1‑94 (MQ=60)
                                   CGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTCGAACCGTTAAGTCGCGGTCGTGCATGT                                                           >  SRR4302540.1526748/1‑94 (MQ=60)
                                    GCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTT                                                          <  SRR4302540.8649495/94‑1 (MQ=60)
                                      AGATTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTT                                                        >  SRR4302540.3784288/1‑94 (MQ=60)
                                       GCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTC                                                       <  SRR4302540.3532114/94‑1 (MQ=60)
                                       GCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTC                                                       <  SRR4302540.609269/94‑1 (MQ=60)
                                        CTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCG                                                      >  SRR4302540.7054349/1‑94 (MQ=60)
                                        CTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCG                                                      >  SRR4302540.7368670/1‑94 (MQ=60)
                                        CTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCG                                                      <  SRR4302540.3221303/94‑1 (MQ=60)
                                        CTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCG                                                      <  SRR4302540.4670540/94‑1 (MQ=60)
                                           TGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTC                                                   >  SRR4302540.2360158/1‑94 (MQ=60)
                                           TGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTC                                                   >  SRR4302540.3447204/1‑94 (MQ=60)
                                            GCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCA                                                  >  SRR4302540.8118795/1‑94 (MQ=60)
                                            GCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCA                                                  <  SRR4302540.8276061/94‑1 (MQ=60)
                                            GCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCA                                                  <  SRR4302540.4284092/94‑1 (MQ=60)
                                             CAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAG                                                 >  SRR4302540.6185640/1‑94 (MQ=60)
                                             CAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGGTATGGCCTACTGGGACTGGAACGGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAG                                                 >  SRR4302540.6185639/1‑94 (MQ=60)
                                              AAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGC                                                <  SRR4302540.6137563/94‑1 (MQ=60)
                                               AGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCG                                               <  SRR4302540.3033072/94‑1 (MQ=60)
                                                 CATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGGCTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCT                                             <  SRR4302540.3072906/94‑1 (MQ=60)
                                                 CATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCT                                             <  SRR4302540.2726421/94‑1 (MQ=60)
                                                 CATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCT                                             >  SRR4302540.6028255/1‑94 (MQ=60)
                                                   TTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAA                                           <  SRR4302540.5072095/94‑1 (MQ=60)
                                                   TTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAA                                           >  SRR4302540.7622563/1‑94 (MQ=60)
                                                    TGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAA                                          >  SRR4302540.5072354/1‑94 (MQ=60)
                                                      CAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAAC                                        <  SRR4302540.2739177/94‑1 (MQ=60)
                                                      CAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAAC                                        >  SRR4302540.7696724/1‑94 (MQ=60)
                                                      CAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAAC                                        <  SRR4302540.3269030/94‑1 (MQ=60)
                                                      CAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAAC                                        >  SRR4302540.1836178/1‑94 (MQ=60)
                                                      CAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAAC                                        >  SRR4302540.1836179/1‑94 (MQ=60)
                                                       AGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACG                                       >  SRR4302540.1286713/1‑94 (MQ=60)
                                                       AGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACG                                       <  SRR4302540.7735659/94‑1 (MQ=60)
                                                       AGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACG                                       >  SRR4302540.6094515/1‑94 (MQ=60)
                                                       AGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACG                                       >  SRR4302540.9210552/1‑94 (MQ=60)
                                                       AGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGGGCATGTTTTCGTccaccgcaaaaact                                       >  SRR4302540.4410219/1‑80 (MQ=60)
                                                         ACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTT                                     >  SRR4302540.6146374/1‑94 (MQ=60)
                                                         ACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTT                                     >  SRR4302540.6146373/1‑94 (MQ=60)
                                                         ACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTT                                     <  SRR4302540.3223841/94‑1 (MQ=60)
                                                         ACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTT                                     >  SRR4302540.7551450/1‑94 (MQ=60)
                                                          CCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTG                                    <  SRR4302540.9980160/94‑1 (MQ=60)
                                                           CATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGC                                   <  SRR4302540.2841825/94‑1 (MQ=60)
                                                            ATCGCTGATGAACAAAAACTTTGTTATGGCCTGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCG                                  <  SRR4302540.2772515/94‑1 (MQ=60)
                                                            ATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCG                                  >  SRR4302540.7069158/1‑94 (MQ=60)
                                                            ATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCG                                  >  SRR4302540.1408549/1‑94 (MQ=60)
                                                            AGCGCTGATGAACAAAAACTTTGTTATGGCCTGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCG                                  <  SRR4302540.1294939/94‑1 (MQ=60)
                                                              CGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCC                                <  SRR4302540.6923403/94‑1 (MQ=60)
                                                               GCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCG                               >  SRR4302540.5823064/1‑94 (MQ=60)
                                                                CTGATGAACAAAAACTTTGTTATGGCCTGCTGGGACTGGAACCGTTAAGTCGCGGTCATGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGG                              >  SRR4302540.1244748/1‑94 (MQ=60)
                                                                CTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGG                              <  SRR4302540.2546776/94‑1 (MQ=60)
                                                                 tttTGAACAAATACTTTGTTTTGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTATCGTTTAGCGCTTATACGTTGCGCCTGA                             >  SRR4302540.9052695/4‑94 (MQ=60)
                                                                 TGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGA                             >  SRR4302540.2769397/1‑94 (MQ=60)
                                                                 TGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGA                             <  SRR4302540.2174458/94‑1 (MQ=60)
                                                                  GATGAACAAAAACTTTGTTATGGCCTGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAG                            <  SRR4302540.152212/94‑1 (MQ=60)
                                                                  GATGAACAAAAACTTTGTTATGGCCTACTGGGGCTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAG                            >  SRR4302540.28331/1‑94 (MQ=60)
                                                                  GATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAG                            >  SRR4302540.36932/1‑94 (MQ=60)
                                                                   ATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGC                           >  SRR4302540.2280294/1‑94 (MQ=60)
                                                                   ATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGC                           >  SRR4302540.9607063/1‑94 (MQ=60)
                                                                     GAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGACGGAGCAT                         >  SRR4302540.262948/1‑94 (MQ=60)
                                                                        CAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCT                      <  SRR4302540.3686708/94‑1 (MQ=60)
                                                                         AAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTG                     >  SRR4302540.5933883/1‑94 (MQ=60)
                                                                         AAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTG                     <  SRR4302540.8817509/94‑1 (MQ=60)
                                                                          AAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGC                    >  SRR4302540.1765862/1‑94 (MQ=60)
                                                                           AAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCG                   >  SRR4302540.9821158/1‑94 (MQ=60)
                                                                           AAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCG                   >  SRR4302540.5436622/1‑94 (MQ=60)
                                                                              CTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTA                <  SRR4302540.7829364/94‑1 (MQ=60)
                                                                              CTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTA                >  SRR4302540.5726867/1‑94 (MQ=60)
                                                                               TTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAA               >  SRR4302540.8574473/1‑94 (MQ=60)
                                                                               TTTGTTATGGCCTACTGGGACTGGAACCGTGAAGTCGCGCTAGTGCATGTTTTCTTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAA               <  SRR4302540.9744808/94‑1 (MQ=60)
                                                                               TTTGGTATGGCCTACTGGGGCTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAA               <  SRR4302540.10025539/94‑1 (MQ=60)
                                                                                 TGTTATGGCCTGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAG             >  SRR4302540.8254336/1‑94 (MQ=60)
                                                                                 TGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAG             <  SRR4302540.6633676/94‑1 (MQ=60)
                                                                                 TGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAG             >  SRR4302540.3615160/1‑94 (MQ=60)
                                                                                 TGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAG             >  SRR4302540.4275563/1‑94 (MQ=60)
                                                                                  GTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGA            <  SRR4302540.9656119/94‑1 (MQ=60)
                                                                                   TTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCTCCGGAGCATGCTGCGGTAAAGAG           <  SRR4302540.6729674/94‑1 (MQ=60)
                                                                                   TTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAG           <  SRR4302540.6681786/94‑1 (MQ=60)
                                                                                   TTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAG           <  SRR4302540.2672232/94‑1 (MQ=60)
                                                                                    TATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGA          <  SRR4302540.4481028/94‑1 (MQ=60)
                                                                                     ATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAG         >  SRR4302540.9084601/1‑94 (MQ=60)
                                                                                        GCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGCGCCA      >  SRR4302540.10072103/1‑94 (MQ=60)
                                                                                        GCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCA      <  SRR4302540.6353077/94‑1 (MQ=60)
                                                                                        GCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCA      >  SRR4302540.10071950/1‑94 (MQ=60)
                                                                                         CCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCAT     >  SRR4302540.4288621/1‑94 (MQ=60)
                                                                                         CCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCAT     >  SRR4302540.1614748/1‑94 (MQ=60)
                                                                                         CCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCAT     >  SRR4302540.2139724/1‑94 (MQ=60)
                                                                                         CCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCCCCGGAGCATGCTGCGGTAAAGAGAGCCAT     <  SRR4302540.6135802/94‑1 (MQ=60)
                                                                                          CTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATG    >  SRR4302540.5067276/1‑94 (MQ=60)
                                                                                          CTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATG    >  SRR4302540.2698579/1‑94 (MQ=60)
                                                                                           TACTGGGGCTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGA   <  SRR4302540.1757055/94‑1 (MQ=60)
                                                                                           TACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGA   >  SRR4302540.7930930/1‑94 (MQ=60)
                                                                                            ACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAG  <  SRR4302540.8022972/94‑1 (MQ=60)
                                                                                            |                                                                                             
GCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAG  >  NC_000913/1821953‑1822138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑