Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,822,045 G→A L169L (CTG→CTA)  b1741 → putative excinuclease subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,822,0450GA100.0% 879.5 / NA 239L169L (CTG→CTA) b1741putative excinuclease subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (129/110);  total (129/110)

Only 100 of 245 total aligned reads displayed.

CCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCA  >  NC_000913/1821955‑1822134
                                                                                          |                                                                                         
ccccAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCctact                                                                                         <  1:3729387/93‑1 (MQ=255)
 cccAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCctactg                                                                                        >  2:3181475/1‑93 (MQ=255)
 cccAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCctactg                                                                                        <  1:441526/93‑1 (MQ=255)
 cccAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCctactg                                                                                        <  1:6102750/93‑1 (MQ=255)
 cccAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCctactg                                                                                        <  1:1812904/93‑1 (MQ=255)
  ccAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTgg                                                                                       >  1:5446491/1‑93 (MQ=255)
  ccAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTgg                                                                                       <  1:6263181/93‑1 (MQ=255)
   cAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTggg                                                                                      >  2:3113364/1‑93 (MQ=255)
   cAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTggg                                                                                      >  2:3382653/1‑93 (MQ=255)
    aaCCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGa                                                                                     >  2:2608951/1‑93 (MQ=255)
    aaCCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGa                                                                                     <  1:1432426/93‑1 (MQ=255)
    aaCCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGa                                                                                     >  1:4636819/1‑93 (MQ=255)
      ccTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACt                                                                                   <  1:2900017/93‑1 (MQ=255)
       cTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTg                                                                                  >  1:1048854/1‑93 (MQ=255)
       cTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTg                                                                                  >  1:2660284/1‑93 (MQ=255)
        tGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTgg                                                                                 >  1:3975749/1‑93 (MQ=255)
        tGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTgg                                                                                 <  2:4505419/93‑1 (MQ=255)
         gTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGa                                                                                >  2:3598726/1‑93 (MQ=255)
         gTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGa                                                                                >  1:5679782/1‑93 (MQ=255)
         gTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGa                                                                                >  2:6065469/1‑93 (MQ=255)
          tttGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGaa                                                                               >  2:4342054/1‑93 (MQ=255)
          tttGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGaa                                                                               <  1:5000944/93‑1 (MQ=255)
           ttGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAAc                                                                              <  1:5110292/93‑1 (MQ=255)
            tGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAAcc                                                                             >  2:141468/1‑93 (MQ=255)
             ggCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCg                                                                            >  2:5890270/1‑93 (MQ=255)
              gCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGt                                                                           >  1:3863182/1‑93 (MQ=255)
                cTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACTATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGGACCGTTa                                                                         >  1:4025538/1‑93 (MQ=255)
                 tGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTaa                                                                        <  2:905839/93‑1 (MQ=255)
                 tGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTaa                                                                        <  1:2087150/93‑1 (MQ=255)
                   tttGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGt                                                                      <  1:1835359/93‑1 (MQ=255)
                    ttGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTc                                                                     >  1:165618/1‑93 (MQ=255)
                    ttGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTc                                                                     <  2:5681582/93‑1 (MQ=255)
                       ccAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTcgcg                                                                  >  2:2238680/1‑93 (MQ=255)
                        cAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCgg                                                                 >  2:6112943/1‑93 (MQ=255)
                         aaTAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGt                                                                <  1:4107827/93‑1 (MQ=255)
                         aaTAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGt                                                                <  1:2096692/93‑1 (MQ=255)
                             ggCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTg                                                            >  2:403676/1‑93 (MQ=255)
                             ggCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTg                                                            <  2:734223/93‑1 (MQ=255)
                              gcgcgcAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGc                                                           <  2:4916554/93‑1 (MQ=255)
                                   cAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGtt                                                      >  2:557622/1‑93 (MQ=255)
                                   cAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGtt                                                      >  2:1356089/1‑93 (MQ=255)
                                   cAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGtt                                                      >  2:5319439/1‑93 (MQ=255)
                                    aGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGttt                                                     <  1:2952261/93‑1 (MQ=255)
                                     gCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGtttt                                                    <  2:4784487/93‑1 (MQ=255)
                                     gCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGtttt                                                    <  1:3429931/93‑1 (MQ=255)
                                     gCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGtttt                                                    >  1:2216025/1‑93 (MQ=255)
                                     gCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGtttt                                                    <  1:4227154/93‑1 (MQ=255)
                                      cTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTc                                                   <  1:3741492/93‑1 (MQ=255)
                                       tttGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCg                                                  <  1:1725257/93‑1 (MQ=255)
                                       tttGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCg                                                  >  1:65841/1‑93 (MQ=255)
                                         tGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGtt                                                >  2:1922522/1‑93 (MQ=255)
                                             aGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAgc                                            <  2:2775198/93‑1 (MQ=255)
                                              gCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAgcg                                           >  2:5036527/1‑93 (MQ=255)
                                                aTTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCt                                         >  2:1050351/1‑93 (MQ=255)
                                                   gCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTaaa                                      <  2:3709736/93‑1 (MQ=255)
                                                   gCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTaaa                                      <  2:3005724/93‑1 (MQ=255)
                                                    cAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTaaaa                                     <  2:4797453/93‑1 (MQ=255)
                                                    cAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTaaaa                                     >  2:1967670/1‑93 (MQ=255)
                                                      gACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACg                                   >  2:4096579/1‑93 (MQ=255)
                                                        ccATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGtt                                 <  1:189023/93‑1 (MQ=255)
                                                          aTCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTgc                               <  2:4444151/93‑1 (MQ=255)
                                                           tCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTgcg                              >  1:1247783/1‑93 (MQ=255)
                                                             gCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGcc                            <  2:540714/93‑1 (MQ=255)
                                                             gCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGcc                            >  1:5722209/1‑93 (MQ=255)
                                                             gCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGcc                            >  1:5258946/1‑93 (MQ=255)
                                                             gCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGcc                            >  2:4243230/1‑93 (MQ=255)
                                                              cTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCg                           >  1:4793973/1‑93 (MQ=255)
                                                               tgatgaACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCgg                          <  1:4278175/93‑1 (MQ=255)
                                                               tgatgaACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCgg                          >  1:5051129/1‑93 (MQ=255)
                                                                gatgaACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGa                         <  2:1582576/93‑1 (MQ=255)
                                                                gatgaACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGa                         >  1:1377464/1‑93 (MQ=255)
                                                                 atgaACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAg                        >  1:4056592/1‑93 (MQ=255)
                                                                 atgaACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAg                        <  2:2357703/93‑1 (MQ=255)
                                                                  tgaACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGc                       <  1:593043/93‑1 (MQ=255)
                                                                  tgaACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGc                       >  1:5389470/1‑93 (MQ=255)
                                                                   gaACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCa                      <  1:2551059/93‑1 (MQ=255)
                                                                   gaACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCa                      <  1:3505125/93‑1 (MQ=255)
                                                                     aCAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCAtg                    <  2:3330460/93‑1 (MQ=255)
                                                                     aCAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCAtg                    >  1:3974278/1‑93 (MQ=255)
                                                                      cAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCAtgc                   <  1:48888/93‑1 (MQ=255)
                                                                       aaaaaCTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCAtgct                  >  1:5601573/1‑93 (MQ=255)
                                                                        aaaaCTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCAtgctg                 <  2:2493272/93‑1 (MQ=255)
                                                                         aagcTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCAtgctgc                <  2:2165823/90‑1 (MQ=255)
                                                                         aaaCTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCAtgctgc                <  1:120622/93‑1 (MQ=255)
                                                                         aaaCTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCAtgctgc                >  2:3528012/1‑93 (MQ=255)
                                                                         aaaCTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCAtgctgc                <  2:2642242/93‑1 (MQ=255)
                                                                          aaCTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCg               >  1:2204498/1‑93 (MQ=255)
                                                                          aaCTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCg               >  1:2675349/1‑93 (MQ=255)
                                                                            cTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGt             >  1:3397878/1‑93 (MQ=255)
                                                                             tttGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTACAACGTTGCGCCGGAGCATGCTGCGGTa            >  1:505437/1‑93 (MQ=255)
                                                                             tttGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTa            >  2:1346787/1‑93 (MQ=255)
                                                                             tttGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTa            <  1:2596269/93‑1 (MQ=255)
                                                                             tttGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTa            <  2:1809254/93‑1 (MQ=255)
                                                                               tGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTaaa          <  2:2911551/93‑1 (MQ=255)
                                                                                gTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAag         <  1:3891671/93‑1 (MQ=255)
                                                                                  tATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAagag       >  2:297203/1‑93 (MQ=255)
                                                                                  tATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAagag       >  2:2763533/1‑93 (MQ=255)
                                                                                      gCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGcc   <  1:3640905/93‑1 (MQ=255)
                                                                                      gCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGcc   <  1:3065975/93‑1 (MQ=255)
                                                                                       ccTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCa  <  1:936015/93‑1 (MQ=255)
                                                                                          |                                                                                         
CCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCA  >  NC_000913/1821955‑1822134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAG  >  NC_000913/1821952‑1822138
                                                                                             |                                                                                             
AGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTA                                                                                               >  SRR4302533.964737/1‑94 (MQ=60)
  CCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACT                                                                                             <  SRR4302533.3452495/94‑1 (MQ=60)
   CCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTG                                                                                            <  SRR4302533.3752265/94‑1 (MQ=60)
    CCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGG                                                                                           >  SRR4302533.5479302/1‑94 (MQ=60)
    CCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGG                                                                                           <  SRR4302533.1823976/94‑1 (MQ=60)
    CCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGG                                                                                           <  SRR4302533.444165/94‑1 (MQ=60)
    CCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGG                                                                                           <  SRR4302533.6139591/94‑1 (MQ=60)
     CCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGG                                                                                          <  SRR4302533.6300617/94‑1 (MQ=60)
     CCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGG                                                                                          >  SRR4302533.4997780/1‑94 (MQ=60)
     CCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGG                                                                                          >  SRR4302533.3449770/1‑94 (MQ=60)
      CAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGA                                                                                         >  SRR4302533.2223063/1‑94 (MQ=60)
      CAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGA                                                                                         >  SRR4302533.4664916/1‑94 (MQ=60)
       AACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGAC                                                                                        <  SRR4302533.3678961/94‑1 (MQ=60)
       AACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGAC                                                                                        <  SRR4302533.1441083/94‑1 (MQ=60)
        ACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACT                                                                                       >  SRR4302533.2026147/1‑94 (MQ=60)
         CCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTG                                                                                      >  SRR4302533.2676620/1‑94 (MQ=60)
         CCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTG                                                                                      <  SRR4302533.2917836/94‑1 (MQ=60)
         CCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTG                                                                                      >  SRR4302533.1055021/1‑94 (MQ=60)
          CTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGG                                                                                     <  SRR4302533.6296733/94‑1 (MQ=60)
          CTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGG                                                                                     >  SRR4302533.3999952/1‑94 (MQ=60)
           TGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGA                                                                                    >  SRR4302533.5714086/1‑94 (MQ=60)
            GTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAA                                                                                   >  SRR4302533.1286992/1‑94 (MQ=60)
            GTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAA                                                                                   <  SRR4302533.1608191/94‑1 (MQ=60)
            GTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAA                                                                                   >  SRR4302533.6279105/1‑94 (MQ=60)
             TTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAAC                                                                                  <  SRR4302533.5031257/94‑1 (MQ=60)
              TTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACC                                                                                 <  SRR4302533.5141176/94‑1 (MQ=60)
               TGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCG                                                                                >  SRR4302533.6072448/1‑94 (MQ=60)
                GGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGT                                                                               >  SRR4302533.3886762/1‑94 (MQ=60)
                GGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGT                                                                               >  SRR4302533.3516547/1‑94 (MQ=60)
                 GCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTT                                                                              <  SRR4302533.5691661/94‑1 (MQ=60)
                  CCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACTATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGGACCGTTA                                                                             >  SRR4302533.4050044/1‑94 (MQ=60)
                   CTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAA                                                                            >  SRR4302533.298988/1‑94 (MQ=60)
                    TGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAG                                                                           <  SRR4302533.2100003/94‑1 (MQ=60)
                    TGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAG                                                                           >  SRR4302533.2963987/1‑94 (MQ=60)
                    TGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAG                                                                           <  SRR4302533.404000/94‑1 (MQ=60)
                      TTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTC                                                                         >  SRR4302533.166670/1‑94 (MQ=60)
                      TTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTC                                                                         <  SRR4302533.1846558/94‑1 (MQ=60)
                        TGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGC                                                                       <  SRR4302533.3832608/94‑1 (MQ=60)
                         GCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCG                                                                      <  SRR4302533.5622474/94‑1 (MQ=60)
                         GCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCG                                                                      <  SRR4302533.389165/94‑1 (MQ=60)
                           CAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGT                                                                    >  SRR4302533.406677/1‑94 (MQ=60)
                           CAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGT                                                                    >  SRR4302533.689936/1‑94 (MQ=60)
                            AATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTC                                                                   <  SRR4302533.4132803/94‑1 (MQ=60)
                            AATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTC                                                                   <  SRR4302533.1216455/94‑1 (MQ=60)
                            AATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTC                                                                   <  SRR4302533.2646781/94‑1 (MQ=60)
                            AATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTC                                                                   <  SRR4302533.4285523/94‑1 (MQ=60)
                            AATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTC                                                                   <  SRR4302533.2109601/94‑1 (MQ=60)
                              TAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGT                                                                 <  SRR4302533.5715028/94‑1 (MQ=60)
                                 GCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCA                                                              <  SRR4302533.861433/94‑1 (MQ=60)
                                  CGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCAT                                                             <  SRR4302533.3279166/94‑1 (MQ=60)
                                  CGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCAT                                                             <  SRR4302533.5513206/94‑1 (MQ=60)
                                     GCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTT                                                          >  SRR4302533.1186125/1‑94 (MQ=60)
                                     GCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTT                                                          >  SRR4302533.2252840/1‑94 (MQ=60)
                                       AGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTT                                                        <  SRR4302533.2970314/94‑1 (MQ=60)
                                       AGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTT                                                        >  SRR4302533.1864918/1‑94 (MQ=60)
                                       AGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTT                                                        <  SRR4302533.4785619/94‑1 (MQ=60)
                                       AGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTT                                                        >  SRR4302533.2229705/1‑94 (MQ=60)
                                        GCTTTGCACGCATTGCAGCCCATCACTGATGAACAATAACTTTGTTATGGCCTACTGGGTCTGGCACCGTTAGGTCGCGGTCGTGCATGTTTTC                                                       <  SRR4302533.6345150/94‑1 (MQ=60)
                                        GCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTC                                                       <  SRR4302533.4252714/94‑1 (MQ=60)
                                        GCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTC                                                       >  SRR4302533.2679177/1‑94 (MQ=60)
                                        GCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTC                                                       <  SRR4302533.3451120/94‑1 (MQ=60)
                                         CTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCG                                                      <  SRR4302533.3764436/94‑1 (MQ=60)
                                         CTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCG                                                      >  SRR4302533.4351092/1‑94 (MQ=60)
                                         CTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCG                                                      >  SRR4302533.66267/1‑94 (MQ=60)
                                          TTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGT                                                     <  SRR4302533.5110254/94‑1 (MQ=60)
                                          TTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGT                                                     <  SRR4302533.1735727/94‑1 (MQ=60)
                                            TGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTC                                                   >  SRR4302533.5463697/1‑94 (MQ=60)
                                               AAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGC                                                >  SRR4302533.2756071/1‑94 (MQ=60)
                                               AAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGC                                                <  SRR4302533.5095412/94‑1 (MQ=60)
                                               AAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGC                                                <  SRR4302533.1670758/94‑1 (MQ=60)
                                                AGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCG                                               <  SRR4302533.354742/94‑1 (MQ=60)
                                                   ATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTA                                            >  SRR4302533.2155062/1‑94 (MQ=60)
                                                   ATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTA                                            >  SRR4302533.1810450/1‑94 (MQ=60)
                                                       CAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAAC                                        <  SRR4302533.183269/94‑1 (MQ=60)
                                                        AGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACG                                       <  SRR4302533.1851594/94‑1 (MQ=60)
                                                        AGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACG                                       <  SRR4302533.4830575/94‑1 (MQ=60)
                                                          ACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTT                                     >  SRR4302533.2581316/1‑94 (MQ=60)
                                                          ACCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTT                                     >  SRR4302533.4750584/1‑94 (MQ=60)
                                                           CTATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTG                                    >  SRR4302533.5373861/1‑94 (MQ=60)
                                                           CCATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTG                                    <  SRR4302533.190201/94‑1 (MQ=60)
                                                             ATCGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCG                                  >  SRR4302533.1255235/1‑94 (MQ=60)
                                                               CGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCC                                >  SRR4302533.5290521/1‑94 (MQ=60)
                                                               CGCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCC                                >  SRR4302533.5756761/1‑94 (MQ=60)
                                                                GCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCG                               <  SRR4302533.1436281/94‑1 (MQ=60)
                                                                GCTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCG                               >  SRR4302533.4823017/1‑94 (MQ=60)
                                                                 CTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGG                              >  SRR4302533.5083953/1‑94 (MQ=60)
                                                                 CTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGG                              >  SRR4302533.5081674/1‑94 (MQ=60)
                                                                 CTGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGG                              >  SRR4302533.4118801/1‑94 (MQ=60)
                                                                  TGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGG                             >  SRR4302533.239683/1‑94 (MQ=60)
                                                                  TGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGA                             <  SRR4302533.3969985/94‑1 (MQ=60)
                                                                  TGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGA                             <  SRR4302533.4303901/94‑1 (MQ=60)
                                                                  TGATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGA                             >  SRR4302533.1385750/1‑94 (MQ=60)
                                                                   GATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAG                            >  SRR4302533.4081286/1‑94 (MQ=60)
                                                                   GATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAG                            >  SRR4302533.2730348/1‑94 (MQ=60)
                                                                    ATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGC                           >  SRR4302533.5421857/1‑94 (MQ=60)
                                                                    ATGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGC                           >  SRR4302533.5914342/1‑94 (MQ=60)
                                                                     TGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCA                          <  SRR4302533.596543/94‑1 (MQ=60)
                                                                     TGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCA                          >  SRR4302533.3761906/1‑94 (MQ=60)
                                                                     TGAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCA                          >  SRR4302533.1787374/1‑94 (MQ=60)
                                                                      GAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCAT                         <  SRR4302533.1383170/94‑1 (MQ=60)
                                                                      GAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCAT                         <  SRR4302533.3526733/94‑1 (MQ=60)
                                                                      GAACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCAT                         <  SRR4302533.2566760/94‑1 (MQ=60)
                                                                       AACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATG                        >  SRR4302533.3998477/1‑94 (MQ=60)
                                                                        ACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGC                       >  SRR4302533.4589456/1‑94 (MQ=60)
                                                                        ACAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGC                       >  SRR4302533.4346068/1‑94 (MQ=60)
                                                                         CAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCT                      <  SRR4302533.1643606/94‑1 (MQ=60)
                                                                         CAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCT                      >  SRR4302533.5635422/1‑94 (MQ=60)
                                                                         CAAAAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCT                      <  SRR4302533.49226/94‑1 (MQ=60)
                                                                            AAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCG                   >  SRR4302533.2691759/1‑94 (MQ=60)
                                                                            AAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCG                   <  SRR4302533.121386/94‑1 (MQ=60)
                                                                            AAACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCG                   >  SRR4302533.2218085/1‑94 (MQ=60)
                                                                              ACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGT                 >  SRR4302533.3418862/1‑94 (MQ=60)
                                                                              ACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGT                 <  SRR4302533.396594/94‑1 (MQ=60)
                                                                              ACTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGT                 >  SRR4302533.5715689/1‑94 (MQ=60)
                                                                               CTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTACAACGTTGCGCCGGAGCATGCTGCGGTA                >  SRR4302533.508436/1‑94 (MQ=60)
                                                                               CTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTA                >  SRR4302533.3703323/1‑94 (MQ=60)
                                                                               CTTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTA                >  SRR4302533.2990790/1‑94 (MQ=60)
                                                                                TTTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAA               <  SRR4302533.2612221/94‑1 (MQ=60)
                                                                                 TTGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAA              <  SRR4302533.2659032/94‑1 (MQ=60)
                                                                                  TGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAG             >  SRR4302533.3999602/1‑94 (MQ=60)
                                                                                  TGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAG             >  SRR4302533.3493976/1‑94 (MQ=60)
                                                                                  TGTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAG             <  SRR4302533.3844063/94‑1 (MQ=60)
                                                                                   GTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGA            >  SRR4302533.4070861/1‑94 (MQ=60)
                                                                                   GTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGA            <  SRR4302533.3915418/94‑1 (MQ=60)
                                                                                   GTTATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGA            >  SRR4302533.2108974/1‑94 (MQ=60)
                                                                                     TATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGA          >  SRR4302533.5400879/1‑94 (MQ=60)
                                                                                      ATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAG         >  SRR4302533.5057102/1‑94 (MQ=60)
                                                                                      ATGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAG         >  SRR4302533.4954684/1‑94 (MQ=60)
                                                                                       TGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGC        >  SRR4302533.4149952/1‑94 (MQ=60)
                                                                                       TGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGC        <  SRR4302533.2141637/94‑1 (MQ=60)
                                                                                       TGGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGC        <  SRR4302533.5168706/94‑1 (MQ=60)
                                                                                        GGCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCC       >  SRR4302533.949377/1‑94 (MQ=60)
                                                                                         GCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCA      <  SRR4302533.3663295/94‑1 (MQ=60)
                                                                                         GCCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCA      <  SRR4302533.3084805/94‑1 (MQ=60)
                                                                                          CCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCAT     <  SRR4302533.941511/94‑1 (MQ=60)
                                                                                          CCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCAT     >  SRR4302533.897425/1‑94 (MQ=60)
                                                                                          CCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCAT     >  SRR4302533.2475216/1‑94 (MQ=60)
                                                                                          CCTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCAT     <  SRR4302533.4144924/94‑1 (MQ=60)
                                                                                           CTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATG    >  SRR4302533.1332968/1‑94 (MQ=60)
                                                                                           CTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATG    >  SRR4302533.2739504/1‑94 (MQ=60)
                                                                                           CTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATG    <  SRR4302533.5898661/94‑1 (MQ=60)
                                                                                           CTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATG    >  SRR4302533.148339/1‑94 (MQ=60)
                                                                                           CTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATG    >  SRR4302533.5083170/1‑94 (MQ=60)
                                                                                           CTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATG    >  SRR4302533.3431231/1‑94 (MQ=60)
                                                                                           CTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATG    <  SRR4302533.3191874/94‑1 (MQ=60)
                                                                                           CTACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAACATGCTGCGGTAAAGAGAGCCATG    <  SRR4302533.297614/94‑1 (MQ=60)
                                                                                            TACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGCTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGA   >  SRR4302533.1062192/1‑94 (MQ=60)
                                                                                             ACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAG  <  SRR4302533.5890903/94‑1 (MQ=60)
                                                                                             ACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAG  >  SRR4302533.376784/1‑94 (MQ=60)
                                                                                             ACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAG  >  SRR4302533.3723405/1‑94 (MQ=60)
                                                                                             ACTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAG  <  SRR4302533.3132511/94‑1 (MQ=60)
                                                                                             |                                                                                             
AGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAG  >  NC_000913/1821952‑1822138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑