Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,827,618 A→G R47R (CGT→CGC)  b1746 ← putative aldehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,827,6180AG99.3% 471.0 / ‑5.2 150R47R (CGT→CGC) b1746putative aldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (64/85);  total (64/86)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 159 total aligned reads displayed.

GGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTT  >  NC_000913/1827528‑1827704
                                                                                          |                                                                                      
ggCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCcgcgcg                                                                                      <  2:7645994/93‑1 (MQ=255)
  cTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCggg                                                                                    >  2:3398270/1‑93 (MQ=255)
  cTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCggg                                                                                    >  1:9320232/1‑93 (MQ=255)
   tttATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGc                                                                                   <  1:7185745/93‑1 (MQ=255)
   tttATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGc                                                                                   <  1:4422804/93‑1 (MQ=255)
   tttATTGCTTTCCAGCAGGTCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGc                                                                                   <  1:2197327/93‑1 (MQ=255)
    ttATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCt                                                                                  <  1:7294902/93‑1 (MQ=255)
     tATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTg                                                                                 >  2:4191096/1‑93 (MQ=255)
      aTTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGc                                                                                >  2:4685217/1‑93 (MQ=255)
       ttGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGcc                                                                               >  1:2347409/1‑93 (MQ=255)
        tGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGccc                                                                              >  1:3022928/1‑93 (MQ=255)
          cTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGa                                                                            <  1:3518828/93‑1 (MQ=255)
           tttCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGAc                                                                           >  2:9495436/1‑93 (MQ=255)
            ttCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACa                                                                          >  1:5196491/1‑93 (MQ=255)
             tCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACaa                                                                         <  2:3381329/93‑1 (MQ=255)
               cagcagTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGc                                                                       >  1:2100121/1‑93 (MQ=255)
                cgcagTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGcc                                                                      <  1:7678944/92‑1 (MQ=255)
                cgcagTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGcc                                                                      <  1:7678945/92‑1 (MQ=255)
                 gcagTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACACGCCt                                                                     >  1:1897618/1‑93 (MQ=255)
                 gcagTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCt                                                                     <  1:4546309/93‑1 (MQ=255)
                  cagTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACCAGCCTg                                                                    >  1:7200311/1‑93 (MQ=255)
                    gTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCt                                                                  <  1:3196597/93‑1 (MQ=255)
                     tGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTc                                                                 <  1:8036114/93‑1 (MQ=255)
                       cGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGa                                                               <  2:1651189/93‑1 (MQ=255)
                        ggCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGAc                                                              <  2:8139448/93‑1 (MQ=255)
                         gCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGAcc                                                             <  2:2431966/93‑1 (MQ=255)
                         gCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGAcc                                                             <  2:7010944/93‑1 (MQ=255)
                           aaaGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTg                                                           <  2:10046090/93‑1 (MQ=255)
                           aaaGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTg                                                           <  1:7586587/93‑1 (MQ=255)
                           aaaGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTg                                                           >  1:5435598/1‑93 (MQ=255)
                            aaGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGa                                                          >  1:651776/1‑93 (MQ=255)
                            aaGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGa                                                          >  1:7033275/1‑93 (MQ=255)
                              gCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGc                                                        <  1:1970053/93‑1 (MQ=255)
                                 ttCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggc                                                     >  1:6690091/1‑93 (MQ=255)
                                 ttCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggc                                                     >  1:6025953/1‑93 (MQ=255)
                                  tCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggca                                                    <  2:1849349/93‑1 (MQ=255)
                                  tCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggca                                                    <  2:7915133/93‑1 (MQ=255)
                                  tCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggca                                                    <  2:1849348/93‑1 (MQ=255)
                                   cGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcat                                                   <  1:50208/93‑1 (MQ=255)
                                    gACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatc                                                  <  2:8319314/93‑1 (MQ=255)
                                     aCAGCGCCATGACGTGCAGCAAATCGAAGCCGCGCCCAGCGCGAAACCGCCGCGCGGGCTGCCGGACAAGCTTGCTCGACCTGAGcggcatcg                                                 <  2:4437494/93‑1 (MQ=255)
                                     aCAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatcg                                                 >  1:2785772/1‑93 (MQ=255)
                                         cGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatcggcat                                             <  1:5803634/93‑1 (MQ=255)
                                          ggCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCggcatcggcatc                                            <  2:4837539/93‑1 (MQ=255)
                                          ggCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCggcatcggcatc                                            >  2:470651/1‑93 (MQ=255)
                                           gCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGcatca                                           <  2:9969377/93‑1 (MQ=255)
                                           gCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGcatca                                           <  1:3632142/93‑1 (MQ=255)
                                            cATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGcatcat                                          <  2:8691634/93‑1 (MQ=255)
                                             aTGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAtt                                         <  1:4084508/93‑1 (MQ=255)
                                             aTGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAtt                                         >  2:5100035/1‑93 (MQ=255)
                                              tGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttg                                        <  1:3855572/93‑1 (MQ=255)
                                              tGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttg                                        >  1:8672182/1‑93 (MQ=255)
                                              tGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttg                                        <  2:3127423/93‑1 (MQ=255)
                                                aCGTTCAGCAAATGAAGGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgcc                                      >  1:9178006/1‑93 (MQ=255)
                                                 cGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgcct                                     <  2:7431854/93‑1 (MQ=255)
                                                  gTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgcctt                                    >  1:8846732/1‑93 (MQ=255)
                                                  gTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgcctt                                    >  2:6880049/1‑93 (MQ=255)
                                                  gTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAACCCTGCTCGACCTCAGTGGCATCCGCATCAttgcctt                                    >  2:5260725/1‑93 (MQ=255)
                                                   ttCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgccttg                                   >  2:8509463/1‑93 (MQ=255)
                                                   ttCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgccttg                                   >  1:1321759/1‑93 (MQ=255)
                                                    tCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgccttgc                                  <  1:8838550/93‑1 (MQ=255)
                                                    tCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgccttgc                                  >  2:7345530/1‑93 (MQ=255)
                                                     cAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgccttgcc                                 >  1:4109359/1‑93 (MQ=255)
                                                      aGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCa                                <  2:2973308/93‑1 (MQ=255)
                                                      aGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCa                                <  2:3440725/93‑1 (MQ=255)
                                                       gCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCAt                               <  1:8165140/93‑1 (MQ=255)
                                                         aaaTGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATaa                             <  1:1176299/93‑1 (MQ=255)
                                                         aaaTGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATaa                             <  1:6536177/93‑1 (MQ=255)
                                                           aTGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAaca                           >  1:1670844/1‑93 (MQ=255)
                                                           aTGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAaca                           >  2:7528762/1‑93 (MQ=255)
                                                            tGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAacac                          <  2:2584079/93‑1 (MQ=255)
                                                             gAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACAcc                         <  2:3744663/93‑1 (MQ=255)
                                                              aaaGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCt                        >  2:3081521/1‑93 (MQ=255)
                                                              aaaGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCt                        <  1:9532022/93‑1 (MQ=255)
                                                              aaaGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCt                        >  1:7102223/1‑93 (MQ=255)
                                                               aaGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTc                       <  2:6268782/93‑1 (MQ=255)
                                                               aaGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTACCTTGCCATAACACCTc                       <  2:2305156/93‑1 (MQ=255)
                                                                aGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTcc                      >  2:7299919/1‑92 (MQ=255)
                                                                 gCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGc                     <  2:941371/93‑1 (MQ=255)
                                                                  ccGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGcc                    <  2:5702669/93‑1 (MQ=255)
                                                                  ccGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGcc                    <  1:5980574/93‑1 (MQ=255)
                                                                   cgcgCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGccc                   <  1:1084517/93‑1 (MQ=255)
                                                                      gcCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGAt                <  2:6785002/93‑1 (MQ=255)
                                                                      gcCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGAt                >  1:4633101/1‑93 (MQ=255)
                                                                       cccAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATa               <  2:2821647/93‑1 (MQ=255)
                                                                         cAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATAcc             >  1:2152812/1‑93 (MQ=255)
                                                                         cAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATAcc             <  2:9194886/93‑1 (MQ=255)
                                                                         cAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATAcc             >  1:2439418/1‑93 (MQ=255)
                                                                          aGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCg            <  1:4146321/93‑1 (MQ=255)
                                                                           gcgcGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCgg           >  1:7618418/1‑93 (MQ=255)
                                                                            cgcgGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGa          <  2:5397868/93‑1 (MQ=255)
                                                                            cgcgGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGa          <  2:6782573/93‑1 (MQ=255)
                                                                              cgGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAAACCCTCGCCCGTTCCCGGAtt        >  2:3308419/1‑93 (MQ=255)
                                                                               ggAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTa       <  1:4748106/93‑1 (MQ=255)
                                                                                gAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTAc      >  2:9396949/1‑93 (MQ=255)
                                                                                  aaCGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGc    >  2:9110105/1‑93 (MQ=255)
                                                                                  aaCGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGc    >  2:4360442/1‑93 (MQ=255)
                                                                                  aaCGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTAGCCCGATACCGGATTACGc    >  2:8845685/1‑93 (MQ=255)
                                                                                   aCGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCt   <  2:888483/93‑1 (MQ=255)
                                                                                    cgccgcGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCtt  <  1:5482984/93‑1 (MQ=255)
                                                                                          |                                                                                      
GGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTT  >  NC_000913/1827528‑1827704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACAC  >  NC_000913/1827525‑1827709
                                                                                             |                                                                                           
TTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCG                                                                                             >  SRR4302540.3628752/1‑94 (MQ=60)
    GCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGG                                                                                         >  SRR4302540.9431436/1‑94 (MQ=60)
      TTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCT                                                                                       <  SRR4302540.7265850/94‑1 (MQ=60)
      TTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCT                                                                                       <  SRR4302540.4470726/94‑1 (MQ=60)
      TTTATTGCTTTCCAGCAGGTCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCT                                                                                       <  SRR4302540.2221057/94‑1 (MQ=60)
      TTTATTGCTTGCCAGCAGTGCGGCAAAGCGTTCGACACCGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCT                                                                                       <  SRR4302540.4470729/94‑1 (MQ=60)
       TTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTG                                                                                      <  SRR4302540.2996803/94‑1 (MQ=60)
       TTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTG                                                                                      <  SRR4302540.7376317/94‑1 (MQ=60)
       TTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTG                                                                                      <  SRR4302540.7376316/94‑1 (MQ=60)
        TATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGC                                                                                     >  SRR4302540.324438/1‑94 (MQ=60)
        TATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGC                                                                                     >  SRR4302540.8973209/1‑94 (MQ=60)
         ATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCC                                                                                    >  SRR4302540.2372695/1‑94 (MQ=60)
          TTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCC                                                                                   >  SRR4302540.3056144/1‑94 (MQ=60)
             CTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGAC                                                                                <  SRR4302540.3557164/94‑1 (MQ=60)
              TTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACA                                                                               >  SRR4302540.5253080/1‑94 (MQ=60)
                 CCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGC                                                                            >  SRR4302540.2122666/1‑94 (MQ=60)
                 CCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGC                                                                            >  SRR4302540.2122664/1‑94 (MQ=60)
                 CCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGC                                                                            >  SRR4302540.8933754/1‑94 (MQ=60)
                 CCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGC                                                                            >  SRR4302540.7627207/1‑94 (MQ=60)
                   CGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCT                                                                          <  SRR4302540.7765815/94‑1 (MQ=60)
                   CGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCT                                                                          <  SRR4302540.7765816/94‑1 (MQ=60)
                   AGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACACGCCT                                                                          >  SRR4302540.1918382/1‑94 (MQ=60)
                    GCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACCAGCCTG                                                                         >  SRR4302540.7280590/1‑94 (MQ=60)
                    GCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTG                                                                         <  SRR4302540.4595497/94‑1 (MQ=60)
                       GTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTC                                                                      <  SRR4302540.3231316/94‑1 (MQ=60)
                        TGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCG                                                                     <  SRR4302540.8129511/94‑1 (MQ=60)
                        TGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCG                                                                     <  SRR4302540.1281052/94‑1 (MQ=60)
                           GGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACC                                                                  >  SRR4302540.9560058/1‑94 (MQ=60)
                            GCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCT                                                                 <  SRR4302540.4982/94‑1 (MQ=60)
                             CAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTG                                                                >  SRR4302540.5495382/1‑94 (MQ=60)
                              AAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGA                                                               >  SRR4302540.7111447/1‑94 (MQ=60)
                              AAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGA                                                               <  SRR4302540.7671780/94‑1 (MQ=60)
                              AAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGA                                                               >  SRR4302540.658710/1‑94 (MQ=60)
                                AGCTTTCGACAACGGCATGACGTTCAGCAAATGAATGCCTCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGC                                                             <  SRR4302540.871214/94‑1 (MQ=60)
                                 GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCG                                                            <  SRR4302540.1991436/94‑1 (MQ=60)
                                   GTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGC                                                          >  SRR4302540.6763954/1‑94 (MQ=60)
                                   GTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGC                                                          >  SRR4302540.6092819/1‑94 (MQ=60)
                                    GTCGACAACGGCATGACGTTCAGCAAATGAACGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGCCACGCCTGCTCGACCTGAGCGGCA                                                         <  SRR4302540.6950482/94‑1 (MQ=60)
                                      CGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATC                                                       <  SRR4302540.50709/94‑1 (MQ=60)
                                       GACAACGGCATGCCGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGACACGCCGCGCGGCATGCACGACAAGCCTGCTCGACCTGAGCGGCATCG                                                      >  SRR4302540.4166600/1‑94 (MQ=60)
                                       GACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCG                                                      >  SRR4302540.2815860/1‑94 (MQ=60)
                                         CAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGC                                                    <  SRR4302540.7756826/94‑1 (MQ=60)
                                         CAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGC                                                    <  SRR4302540.1995652/94‑1 (MQ=60)
                                          AACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCA                                                   <  SRR4302540.5552056/94‑1 (MQ=60)
                                           ACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCAT                                                  <  SRR4302540.9718139/94‑1 (MQ=60)
                                            CGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATC                                                 <  SRR4302540.5867760/94‑1 (MQ=60)
                                              GCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAT                                               <  SRR4302540.3671898/94‑1 (MQ=60)
                                                ATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTG                                             >  SRR4302540.859768/1‑94 (MQ=60)
                                                ATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTG                                             >  SRR4302540.8772917/1‑94 (MQ=60)
                                                ATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTG                                             <  SRR4302540.4128555/94‑1 (MQ=60)
                                                ATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCCGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTG                                             <  SRR4302540.6724463/94‑1 (MQ=60)
                                                 TGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGC                                            <  SRR4302540.6451628/94‑1 (MQ=60)
                                                 TGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGC                                            <  SRR4302540.3897492/94‑1 (MQ=60)
                                                  GACGTTCAGCAAATGAAGGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCC                                           >  SRR4302540.9288080/1‑94 (MQ=60)
                                                    CGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTT                                         >  SRR4302540.8949073/1‑94 (MQ=60)
                                                     GTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTG                                        <  SRR4302540.5452854/94‑1 (MQ=60)
                                                     GTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTG                                        >  SRR4302540.3685960/1‑94 (MQ=60)
                                                     GTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTG                                        >  SRR4302540.1335529/1‑94 (MQ=60)
                                                       TCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCC                                      >  SRR4302540.4153716/1‑94 (MQ=60)
                                                       TCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCC                                      <  SRR4302540.8940779/94‑1 (MQ=60)
                                                          GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATA                                   <  SRR4302540.8259953/94‑1 (MQ=60)
                                                            AAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAAC                                 <  SRR4302540.1188458/94‑1 (MQ=60)
                                                            AAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAAC                                 <  SRR4302540.6608719/94‑1 (MQ=60)
                                                            AAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAAC                                 <  SRR4302540.4787811/94‑1 (MQ=60)
                                                            AAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCCGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTTCCATAAC                                 >  SRR4302540.7796509/1‑94 (MQ=60)
                                                             AATGAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACA                                >  SRR4302540.1689015/1‑94 (MQ=60)
                                                                GAAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCT                             >  SRR4302540.7181245/1‑94 (MQ=60)
                                                                 AAAGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTC                            <  SRR4302540.9644862/94‑1 (MQ=60)
                                                                   AGCCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGC                          >  SRR4302540.2232131/1‑94 (MQ=60)
                                                                     CCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCC                        <  SRR4302540.6046797/94‑1 (MQ=60)
                                                                     CCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCC                        <  SRR4302540.6046796/94‑1 (MQ=60)
                                                                     CCGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCC                        <  SRR4302540.180262/94‑1 (MQ=60)
                                                                      CGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCG                       <  SRR4302540.1095638/94‑1 (MQ=60)
                                                                      CGCGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCG                       >  SRR4302540.3979708/1‑94 (MQ=60)
                                                                      CGCGCCCAGCGCGGAAACGCCGCGCGGGCCGCCCGACAAGCCTGCTCGACCTGAGGGGCATCGGCATCATTGCCTTCCCATAACACCTCGCCCG                       >  SRR4302540.5730766/1‑94 (MQ=60)
                                                                        CGCCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGAT                     >  SRR4302540.4682716/1‑94 (MQ=60)
                                                                          CCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATAC                   >  SRR4302540.2789596/1‑94 (MQ=60)
                                                                          CCCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATAC                   <  SRR4302540.7264207/94‑1 (MQ=60)
                                                                           CCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACC                  >  SRR4302540.2465682/1‑94 (MQ=60)
                                                                           CCAGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACC                  >  SRR4302540.2175970/1‑94 (MQ=60)
                                                                             AGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGG                <  SRR4302540.4191195/94‑1 (MQ=60)
                                                                             AGCGCGGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGG                >  SRR4302540.7704073/1‑94 (MQ=60)
                                                                                  GGAAACGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTAC           <  SRR4302540.4798773/94‑1 (MQ=60)
                                                                                  GGAAACGCCGCGCGGGCCGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTAC           >  SRR4302540.6778888/1‑94 (MQ=60)
                                                                                       CGCCGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTC      <  SRR4302540.5543346/94‑1 (MQ=60)
                                                                                          CGCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACA   >  SRR4302540.9299669/1‑94 (MQ=60)
                                                                                           GCGCGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACAC  <  SRR4302540.8756445/94‑1 (MQ=60)
                                                                                             |                                                                                           
TTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACAC  >  NC_000913/1827525‑1827709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑