Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,837,903 A→G K138R (AAA→AGA)  b1757 → putative thiosulfate sulfur transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,837,9030AG100.0% 790.6 / NA 241K138R (AAA→AGA) b1757putative thiosulfate sulfur transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (121/120);  total (121/120)

Only 100 of 246 total aligned reads displayed.

TCGACGCCGTCAAAACGCGACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAAACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACCTGCCGGTGACT  >  NC_000913/1837813‑1837992
                                                                                          |                                                                                         
tCGACGCCGTCAAAACGCGACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGAc                                                                                         >  2:5326462/1‑93 (MQ=255)
 cGACGCCGTCAAAACGCGACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACt                                                                                        <  1:9096992/93‑1 (MQ=255)
 cGACGCCGTCAAAACGCGACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACt                                                                                        <  1:7273845/93‑1 (MQ=255)
 cGACGCCGTCAAAACGCGACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACt                                                                                        <  1:6121929/93‑1 (MQ=255)
 cGACGCCGTCAAAACGCGACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCCGCAAAGACt                                                                                        <  1:6664901/93‑1 (MQ=255)
    cGCCGTCAAAACGCGACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGcc                                                                                     <  2:9786200/93‑1 (MQ=255)
     gCCGTCAAAACGCGACTGCAAAAAGCTGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCg                                                                                    <  2:2484339/93‑1 (MQ=255)
      ccGTCAAAACGCGACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGc                                                                                   <  2:7250511/93‑1 (MQ=255)
         tCAAAACGCGACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCAtt                                                                                >  2:5377276/1‑93 (MQ=255)
               cgcgACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGagc                                                                          <  1:4995975/93‑1 (MQ=255)
                gcgACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTTCAAAGACTGCCGCATTTTGagca                                                                         >  2:5600807/1‑93 (MQ=255)
                gcgACTGCAAAAAGCAGGGTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACATTCCCGTCTGCCAAGACTGCCGCATTCTGagcg                                                                         >  2:4288209/1‑92 (MQ=255)
                  gACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGagcagc                                                                       >  2:3168764/1‑93 (MQ=255)
                  gACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGagcagc                                                                       <  1:3821925/93‑1 (MQ=255)
                  gACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGagcagc                                                                       >  2:9433401/1‑93 (MQ=255)
                   aCTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCt                                                                      <  2:1179482/93‑1 (MQ=255)
                   aCTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCt                                                                      >  1:1872741/1‑93 (MQ=255)
                    cTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTg                                                                     <  2:3556131/93‑1 (MQ=255)
                    cTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTg                                                                     >  1:2409434/1‑93 (MQ=255)
                    cTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTg                                                                     >  2:8363016/1‑93 (MQ=255)
                       cAAAAAGCAGGTTTAACGCATTTCGCCATCCTGAGTGGCGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGtt                                                                  <  2:8255899/93‑1 (MQ=255)
                        aaaaaGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGttt                                                                 >  2:6686707/1‑93 (MQ=255)
                         aaaaGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTa                                                                <  1:9697303/93‑1 (MQ=255)
                         aaaaGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTa                                                                >  2:3991539/1‑93 (MQ=255)
                          aaaGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTAt                                                               >  1:5703404/1‑93 (MQ=255)
                          aaaGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTAt                                                               >  2:5445619/1‑93 (MQ=255)
                              cAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGc                                                           >  2:6395643/1‑93 (MQ=255)
                              cAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGc                                                           <  1:3714577/93‑1 (MQ=255)
                                ggTTTCCCGCTTCTCTCCCTCCTGAGTGCCGCGCTCCGCGGACCTTCCCGTCTGCCACGCCTGCCGCGTTTTGCGCAGCTGGTTTATCCGccc                                                         <  1:7702125/93‑3 (MQ=255)
                                ggTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCaa                                                         <  2:6458415/93‑1 (MQ=255)
                                 gTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAAt                                                        <  1:7132821/93‑1 (MQ=255)
                                 gTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAAt                                                        >  1:2889303/1‑93 (MQ=255)
                                  tttAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATg                                                       <  1:7471302/93‑1 (MQ=255)
                                  tttAACGCATATCTCCATCCTGAGTGACGCCCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATg                                                       <  1:8524232/93‑1 (MQ=255)
                                   ttAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATgg                                                      <  2:9327301/93‑1 (MQ=255)
                                    tAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGc                                                     >  2:3431430/1‑93 (MQ=255)
                                      aCGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTg                                                   >  1:2470137/1‑93 (MQ=255)
                                       cGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGc                                                  <  2:1644258/93‑1 (MQ=255)
                                         cATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCAc                                                >  1:1264692/1‑93 (MQ=255)
                                         cATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCAc                                                <  1:6786092/93‑1 (MQ=255)
                                         cATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCAc                                                <  2:3070168/93‑1 (MQ=255)
                                          atatCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACg                                               >  1:1009006/1‑93 (MQ=255)
                                           tatCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGa                                              >  2:2213920/1‑93 (MQ=255)
                                           tatCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGa                                              <  1:4067300/93‑1 (MQ=255)
                                            atCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGAc                                             <  2:8921563/93‑1 (MQ=255)
                                            atCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGAc                                             <  1:8885355/93‑1 (MQ=255)
                                            atCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGAc                                             <  2:5732625/93‑1 (MQ=255)
                                             tctcCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGAcc                                            >  1:4848089/1‑93 (MQ=255)
                                              ctcCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCt                                           >  2:8692806/1‑93 (MQ=255)
                                               tcCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTg                                          >  1:1241901/1‑93 (MQ=255)
                                                ccATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGc                                         >  1:1456490/1‑93 (MQ=255)
                                                ccATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGc                                         >  1:5028820/1‑93 (MQ=255)
                                                  ctCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGcaa                                       <  1:1631729/92‑1 (MQ=255)
                                                  aTCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGcaa                                       >  1:8785333/1‑93 (MQ=255)
                                                  aTCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGcaa                                       >  2:4501989/1‑93 (MQ=255)
                                                   tCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGcaac                                      <  2:4716003/93‑1 (MQ=255)
                                                     cTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGcaacaa                                    <  1:4874412/93‑1 (MQ=255)
                                                     cTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGcaacaa                                    >  1:6217799/1‑93 (MQ=255)
                                                       gAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACACgg                                  >  1:3482319/1‑93 (MQ=255)
                                                        aGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGt                                 <  2:3314178/93‑1 (MQ=255)
                                                          tGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTaa                               >  2:8766289/1‑93 (MQ=255)
                                                          tGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTaa                               >  1:6320028/1‑93 (MQ=255)
                                                           gACGCGCTAAGCGACCCTTCCCGTCTGCAAAGACTGCCGCATTGTGAGCAGCTGGTTTAGCCGCAATGGCTGCACGACCTGCAACAAGGTaaa                              <  2:5135066/93‑1 (MQ=255)
                                                           gACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTaaa                              >  2:2643334/1‑93 (MQ=255)
                                                            aCGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAag                             <  2:6572507/93‑1 (MQ=255)
                                                            aCGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAag                             <  1:5808731/93‑1 (MQ=255)
                                                             cgcgCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAaga                            <  2:997196/93‑1 (MQ=255)
                                                              gcgcTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAagag                           >  1:9272976/1‑93 (MQ=255)
                                                              gcgcTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAagag                           <  1:5205168/93‑1 (MQ=255)
                                                               cgcTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAgg                          >  2:1840760/1‑93 (MQ=255)
                                                                gcTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGt                         <  2:7423597/93‑1 (MQ=255)
                                                                 cTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGtt                        >  2:9634665/1‑93 (MQ=255)
                                                                 cTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGtt                        <  1:5377277/93‑1 (MQ=255)
                                                                  tAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTa                       <  1:8476980/93‑1 (MQ=255)
                                                                  tAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTa                       >  1:8391729/1‑93 (MQ=255)
                                                                    aGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACg                     <  1:3000248/93‑1 (MQ=255)
                                                                     gCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGGTACgg                    >  1:4191345/1‑93 (MQ=255)
                                                                     gCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGGAAAGAGGTTACgg                    >  1:7682708/1‑93 (MQ=255)
                                                                      cGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGc                   <  1:802860/93‑1 (MQ=255)
                                                                           cTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAAc              <  1:1955566/93‑1 (MQ=255)
                                                                           cTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAAc              >  1:4404357/1‑93 (MQ=255)
                                                                           cTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAAc              <  2:7809663/93‑1 (MQ=255)
                                                                            ttCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGATTACGGCGAAAcc             <  1:8006723/93‑1 (MQ=255)
                                                                               ccGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACCTGc          >  2:2397722/1‑93 (MQ=255)
                                                                               ccGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACCTGc          <  2:3715852/93‑1 (MQ=255)
                                                                               ccGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACCTGc          >  2:815188/1‑93 (MQ=255)
                                                                                cGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACCTGcc         >  2:1072593/1‑93 (MQ=255)
                                                                                  tCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACCTGCCgg       <  2:6998789/93‑1 (MQ=255)
                                                                                  tCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACCTGCCgg       >  1:5413995/1‑93 (MQ=255)
                                                                                  tCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACCTGCCgg       <  1:7623715/93‑1 (MQ=255)
                                                                                   cTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCCGCAACAAGGTAAAGAGGTTACGGCGAAACCTGCCGGt      <  2:4224554/93‑1 (MQ=255)
                                                                                   cTGCAAAGACTGCCGCATTTTGAGCAGCTGGCTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACCGCGAAAACTGccagg      >  2:280816/1‑90 (MQ=255)
                                                                                    tGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACCTGCCGGTg     >  1:7501726/1‑93 (MQ=255)
                                                                                     gCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACCTGCCGGTGa    >  1:3943885/1‑93 (MQ=255)
                                                                                     gCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACCTGCCGGTGa    >  2:1525616/1‑93 (MQ=255)
                                                                                     gCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACCTGCCGGTGa    <  1:6446391/93‑1 (MQ=255)
                                                                                     gCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACCTGCCGGTGa    >  2:9967993/1‑93 (MQ=255)
                                                                                      cAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGCAGTTACGGCGAACCCTGCCGGAGAc   >  2:5224514/1‑93 (MQ=255)
                                                                                      cAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACCTGCCGGTGAc   >  2:9059187/1‑93 (MQ=255)
                                                                                      cAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGATGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACCTGCCGGTGAc   <  1:8441912/93‑1 (MQ=255)
                                                                                          |                                                                                         
TCGACGCCGTCAAAACGCGACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAAACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACCTGCCGGTGACT  >  NC_000913/1837813‑1837992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGTCGACGCCGTCAAAACGCGACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAAACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACCTGCCGGTGACTGGAA  >  NC_000913/1837810‑1837996
                                                                                             |                                                                                             
GTGTCGACGCCGTCAAAACGCGACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAG                                                                                               <  SRR4302540.7784738/94‑1 (MQ=60)
ATGTCGACGCCGTCAAAACGCGACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAG                                                                                               >  SRR4302540.3565060/1‑94 (MQ=60)
ATGTCGACGCCGTCAAAACGCGACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAG                                                                                               >  SRR4302540.3335168/1‑94 (MQ=60)
ATGTCGACGCCGTCAAAACGCGACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAG                                                                                               >  SRR4302540.2250025/1‑94 (MQ=60)
ATGTCGACGCCGTCAAAACGCGACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAG                                                                                               >  SRR4302540.1688260/1‑94 (MQ=60)
  GTCGACGCCGTCAAAACGCGACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGAC                                                                                             <  SRR4302540.2946978/94‑1 (MQ=60)
  GTCGACGCCGTCAAAACGCGACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGAC                                                                                             <  SRR4302540.2946977/94‑1 (MQ=60)
   TCGACGCCGTCAAAACGCGACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACT                                                                                            <  SRR4302540.2809131/94‑1 (MQ=60)
   TCGACGCCGTCAAAACGCGACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACT                                                                                            <  SRR4302540.4450083/94‑1 (MQ=60)
    CGACGCCGTCAAAACGCGACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTG                                                                                           <  SRR4302540.7355010/94‑1 (MQ=60)
    CGACGCCGTCAAAACGCGACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTG                                                                                           <  SRR4302540.9206294/94‑1 (MQ=60)
    CGACGCCGTCAAAACGCGACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTG                                                                                           <  SRR4302540.6190112/94‑1 (MQ=60)
    CGACGCCGTCAAAACGCGACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTG                                                                                           <  SRR4302540.8032505/94‑1 (MQ=60)
    CGACGCCGTCAAAACGCGACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCCGCAAAGACTG                                                                                           <  SRR4302540.6738570/94‑1 (MQ=60)
         CCGTCAAAACGCGACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCA                                                                                      >  SRR4302540.9354043/1‑94 (MQ=60)
           GTCAAAACGCGACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATT                                                                                    >  SRR4302540.8540405/1‑94 (MQ=60)
             CAAAACGCGACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTT                                                                                  >  SRR4302540.292744/1‑94 (MQ=60)
              AAAACGCGACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTG                                                                                 >  SRR4302540.4981289/1‑94 (MQ=60)
               AAACGCGACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGA                                                                                >  SRR4302540.6347014/1‑94 (MQ=60)
                 ACGCGACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGC                                                                              <  SRR4302540.2470325/94‑1 (MQ=60)
                  CGCGACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCA                                                                             <  SRR4302540.5050061/94‑1 (MQ=60)
                  CGCGACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCA                                                                             <  SRR4302540.5050060/94‑1 (MQ=60)
                     GACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCT                                                                          <  SRR4302540.3863523/94‑1 (MQ=60)
                     GACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCT                                                                          >  SRR4302540.1377646/1‑94 (MQ=60)
                     GACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCT                                                                          <  SRR4302540.3788749/94‑1 (MQ=60)
                     GACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCT                                                                          >  SRR4302540.1893258/1‑94 (MQ=60)
                     GACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCT                                                                          >  SRR4302540.7025863/1‑94 (MQ=60)
                     GACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCT                                                                          >  SRR4302540.1395697/1‑94 (MQ=60)
                     GACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGACGCATTTTGAGCAGCG                                                                          >  SRR4302540.1893256/1‑94 (MQ=60)
                      ACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTG                                                                         >  SRR4302540.2435410/1‑94 (MQ=60)
                      ACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTG                                                                         >  SRR4302540.496191/1‑94 (MQ=60)
                       CTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGG                                                                        >  SRR4302540.8112121/1‑94 (MQ=60)
                       CTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGAGCTAAGCGAACCTTCCCGTCTGAAAAGACTGCCGCATTTTGAGCAGCTGG                                                                        >  SRR4302540.5903557/1‑94 (MQ=60)
                        TGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGT                                                                       >  SRR4302540.7788631/1‑94 (MQ=60)
                          CAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTT                                                                     >  SRR4302540.4019624/1‑94 (MQ=60)
                            AAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTAT                                                                   <  SRR4302540.9525157/94‑1 (MQ=60)
                            AAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTAT                                                                   >  SRR4302540.5766202/1‑94 (MQ=60)
                            AAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTAT                                                                   <  SRR4302540.9811322/94‑1 (MQ=60)
                              AAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCC                                                                 >  SRR4302540.6707107/1‑94 (MQ=60)
                               AGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCG                                                                >  SRR4302540.2782785/1‑94 (MQ=60)
                               AGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCG                                                                <  SRR4302540.8538429/94‑1 (MQ=60)
                                 CAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCA                                                              >  SRR4302540.9007999/1‑94 (MQ=60)
                                 CAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCA                                                              <  SRR4302540.3755109/94‑1 (MQ=60)
                                  AGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAA                                                             >  SRR4302540.527733/1‑94 (MQ=60)
                                   GGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAAT                                                            >  SRR4302540.2920774/1‑94 (MQ=60)
                                   GGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAAT                                                            <  SRR4302540.8955268/94‑1 (MQ=60)
                                   GGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAAT                                                            <  SRR4302540.4540256/94‑1 (MQ=60)
                                   GGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAAT                                                            <  SRR4302540.2342245/94‑1 (MQ=60)
                                   GGTTTAACGCATATCTCCATCCGGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAAT                                                            <  SRR4302540.1627929/94‑1 (MQ=60)
                                    GTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATG                                                           >  SRR4302540.8598342/1‑94 (MQ=60)
                                    GTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATG                                                           <  SRR4302540.7212279/94‑1 (MQ=60)
                                     TTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGG                                                          <  SRR4302540.7554665/94‑1 (MQ=60)
                                     TTTAACGCATATCTCCATCCTGAGTGACGCCCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGG                                                          <  SRR4302540.8623283/94‑1 (MQ=60)
                                      TTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGC                                                         <  SRR4302540.7679669/94‑1 (MQ=60)
                                       TAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCT                                                        <  SRR4302540.4681424/94‑1 (MQ=60)
                                        AACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTG                                                       >  SRR4302540.2496729/1‑94 (MQ=60)
                                          CGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCA                                                     >  SRR4302540.6686404/1‑94 (MQ=60)
                                           GCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCAC                                                    >  SRR4302540.1277691/1‑94 (MQ=60)
                                           GCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATGTTGAGCAGCTGGTGTATGCGCAATGGCTGCAC                                                    >  SRR4302540.1277690/1‑94 (MQ=60)
                                            CATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACG                                                   <  SRR4302540.6861128/94‑1 (MQ=60)
                                            CATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACG                                                   >  SRR4302540.8393285/1‑94 (MQ=60)
                                            CATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACG                                                   >  SRR4302540.1019239/1‑94 (MQ=60)
                                            CATAGCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACG                                                   <  SRR4302540.6861127/94‑1 (MQ=60)
                                             ATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGA                                                  >  SRR4302540.5841876/1‑94 (MQ=60)
                                             ATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGA                                                  >  SRR4302540.4131605/1‑94 (MQ=60)
                                              TATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGAC                                                 <  SRR4302540.4111145/94‑1 (MQ=60)
                                               ATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACC                                                >  SRR4302540.7247209/1‑94 (MQ=60)
                                               ATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACC                                                >  SRR4302540.5970272/1‑94 (MQ=60)
                                               ATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACC                                                <  SRR4302540.8988206/94‑1 (MQ=60)
                                               ATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACC                                                >  SRR4302540.4900029/1‑94 (MQ=60)
                                               ATCTACATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACC                                                >  SRR4302540.7069452/1‑94 (MQ=60)
                                                 CTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTG                                              >  SRR4302540.1254572/1‑94 (MQ=60)
                                                  TCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGC                                             >  SRR4302540.1472333/1‑94 (MQ=60)
                                                  TCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGC                                             >  SRR4302540.5083241/1‑94 (MQ=60)
                                                    CATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAA                                           >  SRR4302540.8887013/1‑94 (MQ=60)
                                                    CATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAA                                           >  SRR4302540.7741017/1‑94 (MQ=60)
                                                     CTCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAAC                                          <  SRR4302540.1649586/94‑1 (MQ=60)
                                                      TCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACA                                         >  SRR4302540.1948693/1‑94 (MQ=60)
                                                       CGTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCACACACTGCCGCATTTTGAGCACCGAGTTTATCCTCAATGGCTccacaacctcacacag                                        >  SRR4302540.298181/1‑78 (MQ=55)
                                                       CCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAA                                        >  SRR4302540.6286978/1‑94 (MQ=60)
                                                       CCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAA                                        >  SRR4302540.6286977/1‑94 (MQ=60)
                                                        CTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAG                                       <  SRR4302540.4926720/94‑1 (MQ=60)
                                                         TGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACACGG                                      >  SRR4302540.3520237/1‑94 (MQ=60)
                                                           AGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTA                                    >  SRR4302540.7536841/1‑94 (MQ=60)
                                                            GTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAA                                   >  SRR4302540.6390407/1‑94 (MQ=60)
                                                              GACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAG                                 <  SRR4302540.7595348/94‑1 (MQ=60)
                                                               GCGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGA                                <  SRR4302540.8258136/94‑1 (MQ=60)
                                                               ACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGA                                <  SRR4302540.5872926/94‑1 (MQ=60)
                                                               ACGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGA                                <  SRR4302540.5438134/94‑1 (MQ=60)
                                                                CGCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAG                               >  SRR4302540.9383850/1‑94 (MQ=60)
                                                                 GCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGG                              <  SRR4302540.8793251/94‑1 (MQ=60)
                                                                 GCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGG                              <  SRR4302540.5261865/94‑1 (MQ=60)
                                                                 GCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGG                              <  SRR4302540.937559/94‑1 (MQ=60)
                                                                 GCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGG                              <  SRR4302540.1743915/94‑1 (MQ=60)
                                                                 GCGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTGTGAGCAGCTGGTTTAGCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGG                              <  SRR4302540.5261864/94‑1 (MQ=60)
                                                                  CGCTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGT                             >  SRR4302540.7578596/1‑94 (MQ=60)
                                                                   GCTAAGCGAACCTTCCCGTCTGAAAAGACTGGCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGCAAAGAGGTT                            >  SRR4302540.3689258/1‑94 (MQ=60)
                                                                    CTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTA                           <  SRR4302540.5436410/94‑1 (MQ=60)
                                                                    CTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTA                           >  SRR4302540.8489116/1‑94 (MQ=60)
                                                                    CTAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTGTGAGCAGCTGGTTTATCCGCAAGGGCTGCACGACCTGCAACAAGGTAAAGAGGTTA                           <  SRR4302540.7378244/94‑1 (MQ=60)
                                                                     TAAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTAC                          <  SRR4302540.8575439/94‑1 (MQ=60)
                                                                      AAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACG                         >  SRR4302540.8645705/1‑94 (MQ=60)
                                                                      AAGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACG                         >  SRR4302540.390107/1‑94 (MQ=60)
                                                                       AGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGCGCAGCTGGTTTAcacggcgtggctgcccgacgtgtaacaaggtaacggtgttactg                        >  SRR4302540.4236929/1‑50 (MQ=60)
                                                                       AGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGG                        <  SRR4302540.3033300/94‑1 (MQ=60)
                                                                       AGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGGTACGG                        >  SRR4302540.4236930/1‑94 (MQ=60)
                                                                       AGCGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGGAAAGAGGTTACGG                        >  SRR4302540.7769736/1‑94 (MQ=60)
                                                                         CGAACCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCG                      <  SRR4302540.811389/94‑1 (MQ=60)
                                                                             CCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAAC                  >  SRR4302540.4452113/1‑94 (MQ=60)
                                                                             CCTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAAC                  >  SRR4302540.3883672/1‑94 (MQ=60)
                                                                              CTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACC                 <  SRR4302540.85600/94‑1 (MQ=60)
                                                                              CTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACC                 <  SRR4302540.2403921/94‑1 (MQ=60)
                                                                              CTTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACC                 <  SRR4302540.1976831/94‑1 (MQ=60)
                                                                               TTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACCT                >  SRR4302540.490648/1‑94 (MQ=60)
                                                                               TTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACCT                >  SRR4302540.340156/1‑94 (MQ=60)
                                                                               TTCCCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGATTACGGCGAAACCT                <  SRR4302540.8099894/94‑1 (MQ=60)
                                                                                  CCGTTTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACCTGCC             <  SRR4302540.3253555/94‑1 (MQ=60)
                                                                                  CCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACCTGCC             <  SRR4302540.1047394/94‑1 (MQ=60)
                                                                                  CCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACCTGCC             <  SRR4302540.5464047/94‑1 (MQ=60)
                                                                                  CCGTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCAGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACCTGCC             <  SRR4302540.8072242/94‑1 (MQ=60)
                                                                                    GTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACCTGCCGG           >  SRR4302540.5473524/1‑94 (MQ=60)
                                                                                    GTCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACCTGCCGG           >  SRR4302540.5473525/1‑94 (MQ=60)
                                                                                     TCTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACCTGCCGGT          <  SRR4302540.7709453/94‑1 (MQ=60)
                                                                                      CTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACCTGCCGGTG         >  SRR4302540.7585549/1‑94 (MQ=60)
                                                                                      CTGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACCTGCCGGTG         >  SRR4302540.912625/1‑94 (MQ=60)
                                                                                       TGCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACCTGCCGGTGA        >  SRR4302540.3986479/1‑94 (MQ=60)
                                                                                       TGCAAAGACTGCCGCATTTTGAGAAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACCTGCCGGTGA        >  SRR4302540.5139220/1‑94 (MQ=60)
                                                                                        GCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACCTGCCGGTGAC       <  SRR4302540.6518151/94‑1 (MQ=60)
                                                                                        GCAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACCTGCCGGTGAC       >  SRR4302540.6472996/1‑94 (MQ=60)
                                                                                         CAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACCTGCCGGTGACT      >  SRR4302540.6283555/1‑94 (MQ=60)
                                                                                         CAAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGATGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACCTGCCGGTGACT      <  SRR4302540.8539907/94‑1 (MQ=60)
                                                                                          AAAGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACCTGCCGGTGACTG     >  SRR4302540.8946917/1‑94 (MQ=60)
                                                                                            AGACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACCTGCCGGTGACTGGA   >  SRR4302540.8628849/1‑94 (MQ=60)
                                                                                             GACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACCTGCCGGTGACTGGAA  >  SRR4302540.4667828/1‑94 (MQ=60)
                                                                                             GACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACCTGCCGGTGACTGGAA  >  SRR4302540.9957552/1‑94 (MQ=60)
                                                                                             |                                                                                             
ATGTCGACGCCGTCAAAACGCGACTGCAAAAAGCAGGTTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTTCCCGTCTGCAAAAACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAACAAGGTAAAGAGGTTACGGCGAAACCTGCCGGTGACTGGAA  >  NC_000913/1837810‑1837996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑