Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,839,484 A→G intergenic (‑51/‑30) b1758 ← / → b1759 putative cytochrome oxidase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,839,4840AG99.3% 952.0 / ‑3.5 296intergenic (‑51/‑30)b1758/b1759putative cytochrome oxidase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (155/139);  total (156/140)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

Only 100 of 298 total aligned reads displayed.

GTTTAATCCGGGGATGAAGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCATTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTTTACTCGCG  >  NC_000913/1839394‑1839573
                                                                                          |                                                                                         
gTTTAATCCGGGGATGAAGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGtt                                                                                         >  1:8595329/1‑93 (MQ=255)
 tttAATCCGGGGATGAAGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGttt                                                                                        >  1:2754278/1‑93 (MQ=255)
    aaTCCGGGGATGAAGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTc                                                                                     <  2:7110787/93‑1 (MQ=255)
     aTCCGTGGATGAAGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCg                                                                                    <  1:3248336/93‑1 (MQ=255)
     aTCCGGGGATGAAGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCg                                                                                    >  2:8430645/1‑93 (MQ=255)
      tCCGGGGATGAAGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGc                                                                                   <  2:1652251/93‑1 (MQ=255)
        cGGGGATGAAGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCtt                                                                                 <  2:4299375/93‑1 (MQ=255)
         ggggATGAAGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTa                                                                                <  1:1495567/93‑1 (MQ=255)
         ggggATGAAGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTa                                                                                <  1:9221209/93‑1 (MQ=255)
            gATGAAGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCt                                                                             <  1:4507873/93‑1 (MQ=255)
             aTGAAGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTg                                                                            >  2:5801042/1‑93 (MQ=255)
              tGAAGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGCTATTATGGCGTTTTTCGCTTATCTGc                                                                           >  1:6688824/1‑93 (MQ=255)
              tGAAGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGc                                                                           >  2:8565147/1‑93 (MQ=255)
               gAAGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGcc                                                                          >  2:412520/1‑93 (MQ=255)
               gAAGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGcc                                                                          <  2:6084253/93‑1 (MQ=255)
                  gATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCgt                                                                       >  2:5892062/1‑93 (MQ=255)
                   aTGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCgtg                                                                      <  1:7078420/93‑1 (MQ=255)
                    tGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCgtgt                                                                     <  1:9146012/93‑1 (MQ=255)
                    tGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCATTTTTCGCTTATCTGCCCgtgt                                                                     >  1:9795097/1‑93 (MQ=255)
                         gTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAAt                                                                >  1:7214176/1‑93 (MQ=255)
                         gTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAAt                                                                >  1:4824158/1‑93 (MQ=255)
                            tAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTa                                                             >  1:7058131/1‑93 (MQ=255)
                             aGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTAt                                                            >  2:7549894/1‑93 (MQ=255)
                              gCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATg                                                           >  2:8867562/1‑93 (MQ=255)
                               cacaGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGa                                                          <  2:10027759/93‑1 (MQ=255)
                               cacaGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGa                                                          <  1:8193632/93‑1 (MQ=255)
                               cacaGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGa                                                          <  2:1888155/93‑1 (MQ=255)
                                acaGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGaa                                                         <  1:8116316/93‑1 (MQ=255)
                                 caGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCgtg                                                                      <  1:6196255/79‑1 (MQ=255)
                                 caGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCgtg                                                                      >  2:6196254/1‑79 (MQ=255)
                                 caGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGaaa                                                        <  1:585868/93‑1 (MQ=255)
                                 caGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGaaa                                                        >  2:4596754/1‑93 (MQ=255)
                                  aGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGaaaa                                                       <  1:5448365/93‑1 (MQ=255)
                                  aGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGaaaa                                                       <  1:5242654/93‑1 (MQ=255)
                                     cACTCCTTAAATATAACGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGa                                                    <  1:8972262/93‑1 (MQ=255)
                                     cACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGa                                                    <  2:2329910/93‑1 (MQ=255)
                                      aCTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGAt                                                   <  1:3980846/93‑1 (MQ=255)
                                       cTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCTTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGAtt                                                  <  2:6880749/93‑1 (MQ=255)
                                       cTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGAtt                                                  >  1:6360545/1‑93 (MQ=255)
                                       cTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGAtt                                                  <  2:4355452/93‑1 (MQ=255)
                                       cTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGAtt                                                  >  2:5375770/1‑93 (MQ=255)
                                        tCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTg                                                 >  2:9675074/1‑93 (MQ=255)
                                        tCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTg                                                 >  2:8387192/1‑93 (MQ=255)
                                        tCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTg                                                 <  1:4705506/93‑1 (MQ=255)
                                         ccTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGa                                                >  1:6566569/1‑93 (MQ=255)
                                          cTTAAATATAAAGCCTTTCTGATTTAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGaa                                               <  1:2587659/93‑1 (MQ=255)
                                          cTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGaa                                               <  1:10039549/93‑1 (MQ=255)
                                          cTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGaa                                               <  2:3539131/93‑1 (MQ=255)
                                           ttAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAg                                              <  1:7287727/93‑1 (MQ=255)
                                            tAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAgt                                             >  2:6339770/1‑93 (MQ=255)
                                            tAAACATAGAGCCTTTCTGATTTAGGAACCGTGCGGATATTATGGCTTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAgt                                             <  1:2139986/93‑1 (MQ=255)
                                             taaTATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAgtt                                            <  1:7355566/92‑1 (MQ=255)
                                               atatAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAgttgt                                          <  1:1816120/93‑1 (MQ=255)
                                                tataAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAgttgtt                                         >  2:7238320/1‑93 (MQ=255)
                                                tataAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAgttgtt                                         >  2:7184916/1‑93 (MQ=255)
                                                aataAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAgttgtt                                         <  1:6933913/92‑1 (MQ=255)
                                                 ataAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGttgttg                                        >  2:8673269/1‑93 (MQ=255)
                                                 ataAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGttgttg                                        >  2:2692361/1‑93 (MQ=255)
                                                  taAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTgc                                       <  2:8210432/93‑1 (MQ=255)
                                                  taAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTgc                                       >  2:1337683/1‑93 (MQ=255)
                                                  taAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTgc                                       <  2:9052608/93‑1 (MQ=255)
                                                   aaaGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTgcc                                      <  2:7739324/93‑1 (MQ=255)
                                                   aaaGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTgcc                                      <  2:5231387/93‑1 (MQ=255)
                                                     aGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTgccgc                                    <  1:7973778/93‑1 (MQ=255)
                                                       ccTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCca                                  <  1:1934501/93‑1 (MQ=255)
                                                        cTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCcat                                 <  2:1181547/93‑1 (MQ=255)
                                                        cTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCcat                                 >  2:2064701/1‑93 (MQ=255)
                                                        cTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGGTGTTGCCGCcat                                 >  2:2764645/1‑93 (MQ=255)
                                                         tttCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCcatc                                >  1:7411435/1‑93 (MQ=255)
                                                         tttCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCcatc                                >  2:2160959/1‑93 (MQ=255)
                                                          ttCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCcatca                               <  2:9236830/93‑1 (MQ=255)
                                                          ttCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCcatca                               <  2:8779642/93‑1 (MQ=255)
                                                          ttCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCcatca                               <  2:4829623/93‑1 (MQ=255)
                                                            cTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCAtt                             >  2:3849080/1‑93 (MQ=255)
                                                            cTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCAtt                             >  1:6439600/1‑93 (MQ=255)
                                                             tGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTg                            >  1:5045298/1‑93 (MQ=255)
                                                              gATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCATGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGa                           >  2:3063513/1‑93 (MQ=255)
                                                               aTTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGaa                          <  1:8401481/93‑1 (MQ=255)
                                                                ttGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAAc                         >  1:455273/1‑93 (MQ=255)
                                                                ttGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAAc                         >  1:1638307/1‑93 (MQ=255)
                                                                ttGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAAc                         >  1:7480926/1‑93 (MQ=255)
                                                                ttGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAAc                         >  1:5126749/1‑93 (MQ=255)
                                                                    gCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGa                     <  2:741690/93‑1 (MQ=255)
                                                                       aCAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATgg                  <  1:9344668/93‑1 (MQ=255)
                                                                       aCAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATgg                  >  2:4904426/1‑93 (MQ=255)
                                                                       aCAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATgg                  >  2:7985057/1‑93 (MQ=255)
                                                                        cAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGc                 >  1:7731777/1‑93 (MQ=255)
                                                                         aGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCa                >  1:564788/1‑93 (MQ=255)
                                                                             cGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAAt            <  1:4249398/93‑1 (MQ=255)
                                                                               gATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAAttt          >  2:1942841/1‑93 (MQ=255)
                                                                               gATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAAttt          >  1:6752513/1‑93 (MQ=255)
                                                                                  attatGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTTCCGCCATCATTGAACGTGATGGCAAAATTTTAc       <  2:5602503/93‑1 (MQ=255)
                                                                                  attatGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTTTAc       >  2:2526749/1‑93 (MQ=255)
                                                                                   ttatGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTTTACt      <  2:3254653/93‑1 (MQ=255)
                                                                                   ttatGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGG‑AAAATTTTAcct     >  1:3341161/1‑91 (MQ=255)
                                                                                    tatGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTTTACTc     >  1:2387272/1‑93 (MQ=255)
                                                                                     atGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTTTACTcg    <  2:3662872/93‑1 (MQ=255)
                                                                                      tGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTTTACTcgc   <  2:1375146/93‑1 (MQ=255)
                                                                                      tGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTTTACTcgc   >  2:147694/1‑93 (MQ=255)
                                                                                       ggCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTTTACTcgcg  >  1:3013417/1‑93 (MQ=255)
                                                                                          |                                                                                         
GTTTAATCCGGGGATGAAGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCATTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTTTACTCGCG  >  NC_000913/1839394‑1839573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACGGTTTAATCCGGGGATGAAGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCATTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTTTACTCGCGCAAC  >  NC_000913/1839391‑1839577
                                                                                             |                                                                                             
ACGGTTTAATCCGGGGATGAAGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCG                                                                                               >  SRR4302540.9821630/1‑94 (MQ=60)
  GGTTTAATCCGGGGATGAAGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTT                                                                                             >  SRR4302540.8695244/1‑94 (MQ=60)
  GGTTTAATCCGGGGATGAAGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTT                                                                                             >  SRR4302540.4256810/1‑94 (MQ=60)
   GTTTAGTCCGGGGATGAAGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTT                                                                                            <  SRR4302540.8996389/94‑1 (MQ=60)
   GTTTAATCCGGGGATGAAGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTT                                                                                            >  SRR4302540.2784044/1‑94 (MQ=60)
   GTTTAATCCGGGGATGAAGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTT                                                                                            >  SRR4302540.3283660/1‑94 (MQ=60)
     TTAATCCGGGGATGAAGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTT                                                                                          <  SRR4302540.9725511/94‑1 (MQ=60)
        ATCCGTGGATGAAGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGC                                                                                       <  SRR4302540.3283713/94‑1 (MQ=60)
        ATCCGGGGATGAAGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGC                                                                                       >  SRR4302540.3290131/1‑94 (MQ=60)
        ATCCGGGGATGAAGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGC                                                                                       >  SRR4302540.4555695/1‑94 (MQ=60)
          CCGGGGATGAAGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTT                                                                                     <  SRR4302540.684087/94‑1 (MQ=60)
            gcttATGAAGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTAT                                                                                   <  SRR4302540.8202157/90‑1 (MQ=60)
            GGGGATGAAGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTAT                                                                                   <  SRR4302540.9331795/94‑1 (MQ=60)
            GGGGATGAAGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTAT                                                                                   <  SRR4302540.1512027/94‑1 (MQ=60)
             GGGATGAAGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCATTTTTCGCTTATC                                                                                  <  SRR4302540.9168565/94‑1 (MQ=60)
              GGATGAAGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCT                                                                                 <  SRR4302540.7507957/94‑1 (MQ=60)
              GGATGAAGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCT                                                                                 <  SRR4302540.8247627/94‑1 (MQ=60)
               GATGAAGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTG                                                                                <  SRR4302540.4556722/94‑1 (MQ=60)
               GATGAAGATGGCGGTATAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTG                                                                                <  SRR4302540.4556721/94‑1 (MQ=60)
                ATGAAGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGCTATTATGGCGTTTTTCGCTTATCTGC                                                                               >  SRR4302540.6762674/1‑94 (MQ=60)
                  GAAGATGGCGGTCTAGCACAGGCACTCCTTAAAGATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCC                                                                             <  SRR4302540.9788021/94‑1 (MQ=60)
                   AAGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCG                                                                            >  SRR4302540.7518464/1‑94 (MQ=60)
                    AGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGT                                                                           >  SRR4302540.2877027/1‑94 (MQ=60)
                     GATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTG                                                                          <  SRR4302540.6613464/94‑1 (MQ=60)
                     GATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTG                                                                          <  SRR4302540.6613463/94‑1 (MQ=60)
                      cgatctagcgcttACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGT                                                                         <  SRR4302540.6265242/81‑1 (MQ=60)
                      ATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGT                                                                         <  SRR4302540.1990787/94‑1 (MQ=60)
                      ATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGT                                                                         <  SRR4302540.7157197/94‑1 (MQ=60)
                      ATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATATGCCCGTGT                                                                         >  SRR4302540.5595925/1‑94 (MQ=60)
                      ATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCATTTTTCGCTTATCTGCCCGTGT                                                                         >  SRR4302540.9909822/1‑94 (MQ=60)
                       TGGCTGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTG                                                                        <  SRR4302540.2686867/94‑1 (MQ=60)
                       TGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTG                                                                        <  SRR4302540.9255865/94‑1 (MQ=60)
                        GGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGT                                                                       >  SRR4302540.7425788/1‑94 (MQ=60)
                        GGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGT                                                                       >  SRR4302540.6476041/1‑94 (MQ=60)
                         GCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTA                                                                      <  SRR4302540.9365714/94‑1 (MQ=60)
                          CGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAA                                                                     >  SRR4302540.151881/1‑94 (MQ=60)
                          CGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAA                                                                     >  SRR4302540.8306825/1‑94 (MQ=60)
                          CGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAA                                                                     >  SRR4302540.3041663/1‑94 (MQ=60)
                           GGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAAT                                                                    >  SRR4302540.8154518/1‑94 (MQ=60)
                           GGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAAT                                                                    >  SRR4302540.4875811/1‑94 (MQ=60)
                           GGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAAT                                                                    >  SRR4302540.4875813/1‑94 (MQ=60)
                           GGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAAT                                                                    <  SRR4302540.1932238/94‑1 (MQ=60)
                           GGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAAT                                                                    >  SRR4302540.7294593/1‑94 (MQ=60)
                           GGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAAT                                                                    >  SRR4302540.4875812/1‑94 (MQ=60)
                            GTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATT                                                                   <  SRR4302540.4592965/94‑1 (MQ=60)
                             TCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTT                                                                  <  SRR4302540.6679702/94‑1 (MQ=60)
                              CTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTA                                                                 >  SRR4302540.7136620/1‑94 (MQ=60)
                                AGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATG                                                               >  SRR4302540.8591727/1‑94 (MQ=60)
                                AGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATG                                                               <  SRR4302540.6400211/94‑1 (MQ=60)
                                 GCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGA                                                              >  SRR4302540.9346812/1‑94 (MQ=60)
                                 GCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGA                                                              >  SRR4302540.1902845/1‑94 (MQ=60)
                                  CACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAA                                                             <  SRR4302540.8288838/94‑1 (MQ=60)
                                  CACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAA                                                             <  SRR4302540.9242225/94‑1 (MQ=60)
                                   ACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAA                                                            <  SRR4302540.8210509/94‑1 (MQ=60)
                                   ACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAA                                                            <  SRR4302540.8210508/94‑1 (MQ=60)
                                    CAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAA                                                           <  SRR4302540.592189/94‑1 (MQ=60)
                                    CAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAA                                                           >  SRR4302540.4915821/1‑94 (MQ=60)
                                     AGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAAT                                                          >  SRR4302540.4842657/1‑94 (MQ=60)
                                     AGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAAT                                                          <  SRR4302540.5508319/94‑1 (MQ=60)
                                     AGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAAT                                                          <  SRR4302540.5299842/94‑1 (MQ=60)
                                     AGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAAT                                                          <  SRR4302540.154844/94‑1 (MQ=60)
                                       GCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGA                                                        >  SRR4302540.560394/1‑94 (MQ=60)
                                        CACTCCTTAAATATAACGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGAT                                                       <  SRR4302540.9078842/94‑1 (MQ=60)
                                        CACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGAT                                                       >  SRR4302540.6827248/1‑94 (MQ=60)
                                        CACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGAT                                                       >  SRR4302540.7234610/1‑94 (MQ=60)
                                         ACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATT                                                      <  SRR4302540.4023723/94‑1 (MQ=60)
                                         ACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATT                                                      >  SRR4302540.6431373/1‑94 (MQ=60)
                                          acCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGGTTTTCGCTTATCTGCCCGTGTGTAATTTATTAAAATGATTG                                                     >  SRR4302540.5723325/3‑94 (MQ=60)
                                          CTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGACTG                                                     >  SRR4302540.1710077/1‑94 (MQ=60)
                                           TCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGA                                                    >  SRR4302540.6639338/1‑94 (MQ=60)
                                           TCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGA                                                    <  SRR4302540.4755786/94‑1 (MQ=60)
                                            CCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAA                                                   <  SRR4302540.7140835/94‑1 (MQ=60)
                                             CTTAAATATAAAGCCTTTCTGATTTAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAG                                                  <  SRR4302540.2615554/94‑1 (MQ=60)
                                             CTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGCAAATGATTGAAT                                                  >  SRR4302540.5174107/1‑94 (MQ=60)
                                             CTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAG                                                  >  SRR4302540.7440737/1‑94 (MQ=60)
                                             CTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAG                                                  <  SRR4302540.10156286/94‑1 (MQ=60)
                                             CTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAG                                                  >  SRR4302540.5478350/1‑94 (MQ=60)
                                              TTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGT                                                 <  SRR4302540.7369050/94‑1 (MQ=60)
                                               TAAACATAGAGCCTTTCTGATTTAGGAACCGTGCGGATATTATGGCTTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTT                                                <  SRR4302540.2162985/94‑1 (MQ=60)
                                                TAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTG                                               <  SRR4302540.7437597/94‑1 (MQ=60)
                                                AAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTG                                               <  SRR4302540.7484131/94‑1 (MQ=60)
                                                AAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTG                                               <  SRR4302540.4728916/94‑1 (MQ=60)
                                                AAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTG                                               >  SRR4302540.7361677/1‑94 (MQ=60)
                                                AAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTG                                               <  SRR4302540.6775952/94‑1 (MQ=60)
                                                 AATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGT                                              <  SRR4302540.2587111/94‑1 (MQ=60)
                                                  ATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTT                                             <  SRR4302540.372058/94‑1 (MQ=60)
                                                  ATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTT                                             <  SRR4302540.1835958/94‑1 (MQ=60)
                                                  ATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTT                                             <  SRR4302540.1835959/94‑1 (MQ=60)
                                                  ATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTT                                             >  SRR4302540.532367/1‑94 (MQ=60)
                                                   TATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTATTTTATGAAAATGATTGAAGTTGTTG                                            <  SRR4302540.594236/94‑1 (MQ=60)
                                                   TATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTG                                            >  SRR4302540.5270365/1‑94 (MQ=60)
                                                   TATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTG                                            <  SRR4302540.7011082/94‑1 (MQ=60)
                                                   TATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTG                                            <  SRR4302540.3374342/94‑1 (MQ=60)
                                                   AATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTG                                            <  SRR4302540.7011080/94‑1 (MQ=60)
                                                     TAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCC                                          >  SRR4302540.646106/1‑94 (MQ=60)
                                                     TAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCC                                          <  SRR4302540.7758007/94‑1 (MQ=60)
                                                     TAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCC                                          <  SRR4302540.8035733/94‑1 (MQ=60)
                                                       AAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGACAATGATTGAAGTTGTTGCCGC                                        >  SRR4302540.7156759/1‑94 (MQ=60)
                                                       AAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGC                                        >  SRR4302540.7373807/1‑94 (MQ=60)
                                                        AGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCC                                       <  SRR4302540.8066679/94‑1 (MQ=60)
                                                         GCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCA                                      <  SRR4302540.1381698/94‑1 (MQ=60)
                                                          CCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCAT                                     <  SRR4302540.1955580/94‑1 (MQ=60)
                                                          CCTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCAT                                     >  SRR4302540.6786217/1‑94 (MQ=60)
                                                          CCTTTCCGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCAT                                     <  SRR4302540.8834654/94‑1 (MQ=60)
                                                           CTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATC                                    >  SRR4302540.7493986/1‑94 (MQ=60)
                                                           CTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATC                                    <  SRR4302540.7126390/94‑1 (MQ=60)
                                                           CTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATC                                    <  SRR4302540.6346652/94‑1 (MQ=60)
                                                           CTTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATC                                    <  SRR4302540.10028285/94‑1 (MQ=60)
                                                            TTTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCA                                   >  SRR4302540.2091708/1‑94 (MQ=60)
                                                             TTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCAT                                  >  SRR4302540.3134535/1‑94 (MQ=60)
                                                             TTCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCAT                                  <  SRR4302540.4124049/94‑1 (MQ=60)
                                                              TCTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATT                                 >  SRR4302540.6511272/1‑94 (MQ=60)
                                                               CTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTG                                >  SRR4302540.5099865/1‑94 (MQ=60)
                                                               CTGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGGAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTG                                >  SRR4302540.5099869/1‑94 (MQ=60)
                                                                TGATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGA                               >  SRR4302540.994561/1‑94 (MQ=60)
                                                                 GATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAA                              <  SRR4302540.2176869/94‑1 (MQ=60)
                                                                  ATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAAC                             >  SRR4302540.460131/1‑94 (MQ=60)
                                                                  ATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAAC                             >  SRR4302540.5182272/1‑94 (MQ=60)
                                                                  ATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAAC                             <  SRR4302540.8498989/94‑1 (MQ=60)
                                                                  ATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAAC                             >  SRR4302540.1656222/1‑94 (MQ=60)
                                                                  ATTGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAAC                             >  SRR4302540.7564425/1‑94 (MQ=60)
                                                                    TGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGT                           >  SRR4302540.9283896/1‑94 (MQ=60)
                                                                    TGAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGT                           >  SRR4302540.2567766/1‑94 (MQ=60)
                                                                     GAGCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTG                          <  SRR4302540.8479712/94‑1 (MQ=60)
                                                                       GCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGAT                        <  SRR4302540.5824406/94‑1 (MQ=60)
                                                                       GCAACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGAT                        >  SRR4302540.8542927/1‑94 (MQ=60)
                                                                          ACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGC                     >  SRR4302540.7356111/1‑94 (MQ=60)
                                                                          ACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGC                     >  SRR4302540.7822033/1‑94 (MQ=60)
                                                                          ACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGC                     <  SRR4302540.9455997/94‑1 (MQ=60)
                                                                          ACAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGC                     <  SRR4302540.5261495/94‑1 (MQ=60)
                                                                           CAGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCA                    >  SRR4302540.570928/1‑94 (MQ=60)
                                                                            AGTGCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAA                   >  SRR4302540.4645307/1‑94 (MQ=60)
                                                                               GCGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAAT                <  SRR4302540.3532741/94‑1 (MQ=60)
                                                                                CGGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATT               <  SRR4302540.4295632/94‑1 (MQ=60)
                                                                                 GGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTT              >  SRR4302540.7617317/1‑94 (MQ=60)
                                                                                 GGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTT              >  SRR4302540.9274648/1‑94 (MQ=60)
                                                                                 GGATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTT              >  SRR4302540.6827126/1‑94 (MQ=60)
                                                                                  GATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTTT             >  SRR4302540.6067039/1‑94 (MQ=60)
                                                                                  GATATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTTT             <  SRR4302540.7978875/94‑1 (MQ=60)
                                                                                    TATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTTTAC           <  SRR4302540.2621303/94‑1 (MQ=60)
                                                                                    TATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTTTAC           <  SRR4302540.4590720/94‑1 (MQ=60)
                                                                                     ATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTTTACT          <  SRR4302540.6735750/94‑1 (MQ=60)
                                                                                     ATTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGG‑AAAATTTTACct         >  SRR4302540.3377829/1‑92 (MQ=60)
                                                                                      TTATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTTTACTC         >  SRR4302540.2413001/1‑94 (MQ=60)
                                                                                       TATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTTTACTCG        >  SRR4302540.7242305/1‑94 (MQ=60)
                                                                                        ATGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTTTACTCGC       <  SRR4302540.2197024/94‑1 (MQ=60)
                                                                                         TGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTTTACTCGCG      >  SRR4302540.3046557/1‑94 (MQ=60)
                                                                                         TGGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTTTACTCGCG      <  SRR4302540.1756730/94‑1 (MQ=60)
                                                                                          GGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCGATCATTGAACGTGATGGCAAAATTTTACTCGCGC     >  SRR4302540.6282109/1‑94 (MQ=60)
                                                                                          GGCGTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTTTACTCGCGC     >  SRR4302540.5900554/1‑94 (MQ=60)
                                                                                             GTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTTTACTCGCGCAAC  >  SRR4302540.8985051/1‑94 (MQ=60)
                                                                                             GTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTTTACTCGCGCAAC  <  SRR4302540.2008690/94‑1 (MQ=60)
                                                                                             GTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTTTACTCGCGCAAC  >  SRR4302540.3119481/1‑94 (MQ=60)
                                                                                             GTTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTTTACTCGCGCAAC  >  SRR4302540.3324869/1‑94 (MQ=60)
                                                                                             |                                                                                             
ACGGTTTAATCCGGGGATGAAGATGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCATTTTTCGCTTATCTGCCCGTGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTTTACTCGCGCAAC  >  NC_000913/1839391‑1839577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑