Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,845,852	T→C	T61A (ACC→GCC)	selD ←	selenophosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,845,852	0	T	C	99.6%	734.9 / ‑4.9	230	T61A (ACC→GCC)	selD	selenophosphate synthase
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (159/70); total (159/71)
Fisher's exact test for biased strand distribution p-value = 3.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.03e-01

Only 100 of 241 total aligned reads displayed.

GCTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTAT  >  NC_000913/1845763‑1845941

gCTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGcc                                                                                        >  1:723574/1‑93 (MQ=255)

   gATGGCGTTAGTCGCCGCAATGCGGCCCAAATCGAAAAGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCAtt                                                                                     >  2:6524185/1‑93 (MQ=255)

   gATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCAtt                                                                                     >  1:6890740/1‑93 (MQ=255)

   gATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCAtt                                                                                     >  2:1643004/1‑93 (MQ=255)

     tGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGc                                                                                   >  2:8615986/1‑93 (MQ=255)

      ggCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGcc                                                                                  <  2:9178585/93‑1 (MQ=255)

      ggCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGcc                                                                                  <  2:7546198/93‑1 (MQ=255)

       gCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGccc                                                                                 >  2:7238044/1‑93 (MQ=255)

        cGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCa                                                                                <  1:4481031/93‑1 (MQ=255)

         gTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAg                                                                               >  1:1979011/1‑93 (MQ=255)

         gTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAg                                                                               >  1:9210632/1‑93 (MQ=255)

         gTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAg                                                                               >  1:10071/1‑93 (MQ=255)

          ttAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGa                                                                              <  1:185490/93‑1 (MQ=255)

          ttAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGa                                                                              >  1:8926014/1‑93 (MQ=255)

          ttAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGa                                                                              >  1:473690/1‑93 (MQ=255)

           tAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGCCGCCATTGACCAGac                                                                             >  2:1814866/1‑92 (MQ=255)

            aGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTAGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATc                                                                            >  2:2668624/1‑93 (MQ=255)

             gTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCg                                                                           >  1:5449820/1‑93 (MQ=255)

               cgccgcAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTa                                                                         <  1:6752100/93‑1 (MQ=255)

               cgccgcAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTa                                                                         <  1:9735317/93‑1 (MQ=255)

                   gcAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACAcc                                                                     >  2:8311041/1‑93 (MQ=255)

                    cAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCCTAAAGAAGTCGGTGGTACTGATAACGCTGGCGACATTGCCCAGATCGTACAgcg                                                                    >  2:3069844/1‑93 (MQ=255)

                    cAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACAccg                                                                    >  1:7918526/1‑93 (MQ=255)

                     aaTGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACAccgc                                                                   <  1:5885636/93‑1 (MQ=255)

                      aTGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACAccgcc                                                                  >  1:572852/1‑93 (MQ=255)

                      aTGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACAccgcc                                                                  >  1:6107170/1‑93 (MQ=255)

                       tGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCAATTGCCCAGATCGCACAccgccg                                                                 >  2:7933590/1‑93 (MQ=255)

                        gCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACcgccgc                                                                <  2:7652672/93‑1 (MQ=255)

                          ggCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCAt                                                              >  1:1308414/1‑93 (MQ=255)

                          ggCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCAt                                                              <  2:5769061/93‑1 (MQ=255)

                          ggCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCAt                                                              >  1:945664/1‑93 (MQ=255)

                             cAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCAtcgt                                                           >  2:5628960/1‑93 (MQ=255)

                              aaaaTCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCAtcgtc                                                          >  2:68354/1‑93 (MQ=255)

                               aaaTCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCAtcgtcg                                                         >  2:5376209/1‑93 (MQ=255)

                               aaaTCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCAtcgtcg                                                         >  1:8409916/1‑93 (MQ=255)

                                 aTCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTcgcg                                                       <  1:251919/93‑1 (MQ=255)

                                    gAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGt                                                    >  2:1884343/1‑93 (MQ=255)

                                    gAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGt                                                    >  2:2534379/1‑93 (MQ=255)

                                    gAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGt                                                    >  2:9423861/1‑93 (MQ=255)

                                    gAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGt                                                    >  2:3619075/1‑93 (MQ=255)

                                    gAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGt                                                    >  1:4357772/1‑93 (MQ=255)

                                    gAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGt                                                    <  1:7199772/93‑1 (MQ=255)

                                    gAAAGGATTATCAACGATCGGCATAAAGAAGTCGGGGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGt                                                    >  2:4271593/1‑93 (MQ=255)

                                      aaGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCCGATCGTACACCGCCGCATCGTCGCGGGttt                                                  >  1:3058043/1‑93 (MQ=255)

                                      aaGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGttt                                                  >  2:8394879/1‑93 (MQ=255)

                                      aaGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGttt                                                  >  1:1167895/1‑93 (MQ=255)

                                       aGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTc                                                 >  1:1328064/1‑93 (MQ=255)

                                           ttATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTa                                             >  1:659251/1‑93 (MQ=255)

                                           ttATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTa                                             <  2:5099324/93‑1 (MQ=255)

                                            tATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTAc                                            <  1:1647766/93‑1 (MQ=255)

                                            tATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTAc                                            <  2:49535/93‑1 (MQ=255)

                                            tATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTAc                                            >  2:5931180/1‑93 (MQ=255)

                                             aTCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGGTTCATTAcc                                           >  2:6261249/1‑93 (MQ=255)

                                              tCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTAccc                                          >  2:4342386/1‑93 (MQ=255)

                                              tCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTAccc                                          >  1:2434870/1‑93 (MQ=255)

                                               cAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCca                                         <  1:7690707/93‑1 (MQ=255)

                                                  cGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACaa                                      <  2:4020118/93‑1 (MQ=255)

                                                   gATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAg                                     >  1:8779827/1‑93 (MQ=255)

                                                    aTCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGc                                    <  1:179778/93‑1 (MQ=255)

                                                     tCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCa                                   >  2:7101011/1‑93 (MQ=255)

                                                         cATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGATTCATTACCCACAAGCAAAtc                               >  1:2140914/1‑92 (MQ=255)

                                                         cATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGACGCATCGTCGCGGGTTTCATTACCCACAAGCAAAtt                               >  2:10046491/1‑93 (MQ=255)

                                                          aTAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTc                              >  1:705453/1‑93 (MQ=255)

                                                            aaaGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCgg                            >  2:8038378/1‑93 (MQ=255)

                                                             aagaagTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGa                           >  1:9534248/1‑93 (MQ=255)

                                                             aagaagTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCAGa                           <  2:4567938/93‑1 (MQ=255)

                                                             aagaagTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGCTTCATTACCCACAAGCAAATTCGGa                           >  1:8479244/1‑93 (MQ=255)

                                                              agaagTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGAt                          >  1:2623910/1‑93 (MQ=255)

                                                               gaagTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACACGCAAATTCGGATc                         >  1:2236096/1‑93 (MQ=255)

                                                               gaagTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATc                         >  2:2935179/1‑93 (MQ=255)

                                                                  gTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATcaac                      <  2:9064690/93‑1 (MQ=255)

                                                                   tCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATcaaca                     >  1:2319942/1‑93 (MQ=255)

                                                                   tCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATcaaca                     <  1:825811/93‑1 (MQ=255)

                                                                   tCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATcaaca                     <  1:995539/93‑1 (MQ=255)

                                                                   tCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATAGTAAACCGCCGCATCGTCGCGGGTATCATTACCCCCAAGCAAATTCCGATcaaca                     >  2:4794947/1‑93 (MQ=255)

                                                                     ggtggtACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACaaa                   >  2:7355887/1‑93 (MQ=255)

                                                                      gtggtACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAAc                  <  2:5466036/93‑1 (MQ=255)

                                                                      gtggtACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAAc                  >  2:4696317/1‑93 (MQ=255)

                                                                       tggtACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCTGATCAACAAACt                 >  2:6299464/1‑93 (MQ=255)

                                                                        ggtACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACtt                <  2:325255/93‑1 (MQ=255)

                                                                         gtACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTc               <  1:4170265/93‑1 (MQ=255)

                                                                         gtACTGATAACGCTGGCGCCATTGCCCAGATAGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTc               <  1:6164888/93‑1 (MQ=255)

                                                                          tACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCg              >  2:2092259/1‑93 (MQ=255)

                                                                          tACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCg              >  1:3781666/1‑93 (MQ=255)

                                                                           aCTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGc             >  2:6955415/1‑93 (MQ=255)

                                                                           aCTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGc             <  1:577844/93‑1 (MQ=255)

                                                                            cTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGcc            >  1:4582764/1‑93 (MQ=255)

                                                                             tGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCGGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCt           >  2:7831922/1‑93 (MQ=255)

                                                                             tGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCt           >  1:3153559/1‑93 (MQ=255)

                                                                              gATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTAATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTg          >  1:7376057/1‑93 (MQ=255)

                                                                              gATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGGTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGACTg          >  2:3729169/1‑93 (MQ=255)

                                                                               aTAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGc         <  2:1366204/93‑1 (MQ=255)

                                                                                tAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCt        <  1:5312126/93‑1 (MQ=255)

                                                                                tAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGCTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCt        >  1:6486250/1‑93 (MQ=255)

                                                                                  aCGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCa      <  2:6492891/93‑1 (MQ=255)

                                                                                  aCGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCa      <  2:1171692/93‑1 (MQ=255)

                                                                                   cGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCAc     >  1:4020300/1‑93 (MQ=255)

                                                                                     cTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTa   <  1:3373531/93‑1 (MQ=255)

                                                                                      tGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTAt  >  2:9511539/1‑93 (MQ=255)

                                                                                      tGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTAt  >  1:1096827/1‑93 (MQ=255)

GCTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTAT  >  NC_000913/1845763‑1845941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TATCGCTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAG  >  NC_000913/1845759‑1845945

TATCGCTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGC                                                                                               >  SRR4302540.3354768/1‑94 (MQ=60)

TATCGCTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTAATAACGCTGGC                                                                                               <  SRR4302540.8638311/94‑1 (MQ=60)

 ATCGCTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCG                                                                                              <  SRR4302540.5140143/94‑1 (MQ=60)

  TCGCTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGC                                                                                             <  SRR4302540.4787034/94‑1 (MQ=60)

   CGCTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCC                                                                                            >  SRR4302540.731329/1‑94 (MQ=60)

     CTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCAT                                                                                          >  SRR4302540.8251524/1‑94 (MQ=60)

      TGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATT                                                                                         <  SRR4302540.1165285/94‑1 (MQ=60)

      TGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATT                                                                                         >  SRR4302540.6967162/1‑94 (MQ=60)

            CGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAG                                                                                   >  SRR4302540.10179/1‑94 (MQ=60)

            CGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAG                                                                                   >  SRR4302540.9321149/1‑94 (MQ=60)

            CGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAG                                                                                   >  SRR4302540.2000483/1‑94 (MQ=60)

            CGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAG                                                                                   <  SRR4302540.4529628/94‑1 (MQ=60)

             GTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGA                                                                                  >  SRR4302540.2552063/1‑94 (MQ=60)

             GTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGA                                                                                  >  SRR4302540.478760/1‑94 (MQ=60)

             GTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGA                                                                                  >  SRR4302540.3242872/1‑94 (MQ=60)

             GTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGA                                                                                  >  SRR4302540.9029834/1‑94 (MQ=60)

              TTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGAT                                                                                 <  SRR4302540.187334/94‑1 (MQ=60)

              TTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGAT                                                                                 <  SRR4302540.187335/94‑1 (MQ=60)

                AGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCG                                                                               >  SRR4302540.9573896/1‑94 (MQ=60)

                AGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCG                                                                               >  SRR4302540.5509791/1‑94 (MQ=60)

                AGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCG                                                                               <  SRR4302540.8573834/94‑1 (MQ=60)

                 GTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGT                                                                              <  SRR4302540.8843190/94‑1 (MQ=60)

                  TCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTA                                                                             <  SRR4302540.1675776/94‑1 (MQ=60)

                   CGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCTGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTAC                                                                            <  SRR4302540.6826709/94‑1 (MQ=60)

                   CGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTAC                                                                            <  SRR4302540.61580/94‑1 (MQ=60)

                   CGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTAC                                                                            <  SRR4302540.6826708/94‑1 (MQ=60)

                   CGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTAC                                                                            >  SRR4302540.890621/1‑94 (MQ=60)

                   CGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTAC                                                                            <  SRR4302540.9849625/94‑1 (MQ=60)

                   CGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTAC                                                                            >  SRR4302540.2154165/1‑94 (MQ=60)

                    GCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACA                                                                           <  SRR4302540.4407258/94‑1 (MQ=60)

                    GCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACA                                                                           <  SRR4302540.5469542/94‑1 (MQ=60)

                    GCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACA                                                                           >  SRR4302540.5652796/1‑94 (MQ=60)

                       GCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCG                                                                        >  SRR4302540.8010830/1‑94 (MQ=60)

                       GCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCG                                                                        <  SRR4302540.6933378/94‑1 (MQ=60)

                        CAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGC                                                                       >  SRR4302540.951059/1‑94 (MQ=60)

                         AATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCC                                                                      >  SRR4302540.252316/1‑94 (MQ=60)

                         AATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCC                                                                      >  SRR4302540.579069/1‑94 (MQ=60)

                         AATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCC                                                                      <  SRR4302540.5950662/94‑1 (MQ=60)

                         AATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCC                                                                      >  SRR4302540.6175190/1‑94 (MQ=60)

                             CGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCAT                                                                  >  SRR4302540.955525/1‑94 (MQ=60)

                             CGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCAT                                                                  <  SRR4302540.6079881/94‑1 (MQ=60)

                             CGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCAT                                                                  >  SRR4302540.7088105/1‑94 (MQ=60)

                             CGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCAT                                                                  >  SRR4302540.1321995/1‑94 (MQ=60)

                             CGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCAT                                                                  >  SRR4302540.1321994/1‑94 (MQ=60)

                              GGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATC                                                                 >  SRR4302540.2212533/1‑94 (MQ=60)

                                CCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGT                                                               <  SRR4302540.1271768/94‑1 (MQ=60)

                                 CAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTC                                                              <  SRR4302540.1620152/94‑1 (MQ=60)

                                 CAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTC                                                              <  SRR4302540.2371534/94‑1 (MQ=60)

                                  AAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCG                                                             >  SRR4302540.7540853/1‑94 (MQ=60)

                                  AAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCG                                                             >  SRR4302540.8507527/1‑94 (MQ=60)

                                    AATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCG                                                           >  SRR4302540.2495032/1‑94 (MQ=60)

                                     ATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGG                                                          <  SRR4302540.254356/94‑1 (MQ=60)

                                       CGACAGGATTTTCAACGATCGGCATAAAGGAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACCCCGCCGCATCGTCGCGGGT                                                        <  SRR4302540.4731945/94‑1 (MQ=60)

                                       CGAAAGGATTATCAACGATCGGCATACAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGT                                                        >  SRR4302540.657746/1‑94 (MQ=60)

                                       CGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGT                                                        >  SRR4302540.4405167/1‑94 (MQ=60)

                                       CGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGT                                                        >  SRR4302540.4405169/1‑94 (MQ=60)

                                       CGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGT                                                        >  SRR4302540.5636859/1‑94 (MQ=60)

                                        GAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTT                                                       <  SRR4302540.7280039/94‑1 (MQ=60)

                                        GAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTT                                                       >  SRR4302540.8378769/1‑94 (MQ=60)

                                        GAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGCACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGCT                                                       >  SRR4302540.7300210/1‑94 (MQ=60)

                                         AAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCCGATCGTACACCGCCGCATCGTCGCGGGTTT                                                      >  SRR4302540.3091595/1‑94 (MQ=60)

                                         AAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTT                                                      >  SRR4302540.3091593/1‑94 (MQ=60)

                                         AAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTT                                                      >  SRR4302540.1179996/1‑94 (MQ=60)

                                          AAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTC                                                     >  SRR4302540.1341933/1‑94 (MQ=60)

                                           AGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCA                                                    >  SRR4302540.5899366/1‑94 (MQ=60)

                                           AGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCA                                                    >  SRR4302540.6275142/1‑94 (MQ=60)

                                           AGCATTATCAACGATCGTGATAAAGAAGTCGCTGGGACTGATAACGCTGGGGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCA                                                    <  SRR4302540.9708923/94‑1 (MQ=60)

                                            GGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCAT                                                   <  SRR4302540.10047614/94‑1 (MQ=60)

                                             GATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATT                                                  >  SRR4302540.4991433/1‑94 (MQ=60)

                                              ATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTA                                                 >  SRR4302540.8514668/1‑94 (MQ=60)

                                              ATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTA                                                 >  SRR4302540.666285/1‑94 (MQ=60)

                                               TTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTAC                                                >  SRR4302540.1334147/1‑94 (MQ=60)

                                               TTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTAC                                                <  SRR4302540.3865062/94‑1 (MQ=60)

                                                TATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACC                                               <  SRR4302540.1665748/94‑1 (MQ=60)

                                                 ATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCC                                              >  SRR4302540.2461097/1‑94 (MQ=60)

                                                  TCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCA                                             >  SRR4302540.4083203/1‑94 (MQ=60)

                                                  TCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCA                                             >  SRR4302540.1881160/1‑94 (MQ=60)

                                                   CAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCAC                                            <  SRR4302540.7778127/94‑1 (MQ=60)

                                                      CGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAG                                         >  SRR4302540.8881485/1‑94 (MQ=60)

                                                       GATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGC                                        >  SRR4302540.4530850/1‑94 (MQ=60)

                                                        ATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCA                                       <  SRR4302540.181565/94‑1 (MQ=60)

                                                          CGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAA                                     >  SRR4302540.9106953/1‑94 (MQ=60)

                                                           GTCATAAAGAAGTCGGTGGTTCTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCTCATCGTCGCGGGTGTCAGTACCCACAAGCAAAT                                    >  SRR4302540.9100069/1‑94 (MQ=60)

                                                            GCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATT                                   >  SRR4302540.1614826/1‑94 (MQ=60)

                                                            GCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCAAAAGCAAATT                                   >  SRR4302540.2261611/1‑94 (MQ=60)

                                                            GCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGATTCATTACCCACAAGCAAATC                                   >  SRR4302540.2163920/1‑94 (MQ=60)

                                                             CATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTC                                  >  SRR4302540.4671239/1‑94 (MQ=60)

                                                             CATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTC                                  >  SRR4302540.713027/1‑94 (MQ=60)

                                                              ATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCG                                 >  SRR4302540.1944264/1‑94 (MQ=60)

                                                              ATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCG                                 >  SRR4302540.4303502/1‑94 (MQ=60)

                                                               TAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGG                                <  SRR4302540.7210314/94‑1 (MQ=60)

                                                               TAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGG                                >  SRR4302540.7914759/1‑94 (MQ=60)

                                                               TAAAGAAGCCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGGTTCATTACCCACAAGCAAATTCGG                                >  SRR4302540.7140842/1‑94 (MQ=60)

                                                                AAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGA                               >  SRR4302540.9647114/1‑94 (MQ=60)

                                                                AAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGCTTCATTACCCACAAGCAAATTCGGA                               >  SRR4302540.8577730/1‑94 (MQ=60)

                                                                 AAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGAT                              <  SRR4302540.5029393/94‑1 (MQ=60)

                                                                 AAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGAT                              >  SRR4302540.2652282/1‑94 (MQ=60)

                                                                  AGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACACGCAAATTCGGATC                             >  SRR4302540.2260150/1‑94 (MQ=60)

                                                                   GAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGGTTCATTACCCACAAGCAAATTCGGATCA                            >  SRR4302540.4140710/1‑94 (MQ=60)

                                                                     AGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAAC                          >  SRR4302540.3757074/1‑94 (MQ=60)

                                                                     AGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAAC                          >  SRR4302540.9590461/1‑94 (MQ=60)

                                                                      GTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACA                         >  SRR4302540.2344922/1‑94 (MQ=60)

                                                                       TCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAA                        <  SRR4302540.834594/94‑1 (MQ=60)

                                                                       TCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAA                        <  SRR4302540.1005677/94‑1 (MQ=60)

                                                                         GGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAAC                      <  SRR4302540.6683286/94‑1 (MQ=60)

                                                                         GGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAAC                      >  SRR4302540.3452789/1‑94 (MQ=60)

                                                                         CGTGGGACTGATCACGCGGGCGCCATTGCCGAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAAC                      <  SRR4302540.7599726/94‑1 (MQ=60)

                                                                           TGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTT                    >  SRR4302540.107095/1‑94 (MQ=60)

                                                                            GGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTC                   >  SRR4302540.5819588/1‑94 (MQ=60)

                                                                             GTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCG                  >  SRR4302540.3822910/1‑94 (MQ=60)

                                                                             GTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCG                  <  SRR4302540.4215497/94‑1 (MQ=60)

                                                                             GTACTGATAACGCTGGCGCCATTGCCCAGATAGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCG                  <  SRR4302540.6233578/94‑1 (MQ=60)

                                                                             GTACTGAGAACGCTGGCCCCATTGCCAAGATCGTCCACCGCCCCATCGGCGCGGGTTTCATTCCCCACAAGCAAATTCGGATCAACAAACTTCG                  <  SRR4302540.6233579/94‑1 (MQ=60)

                                                                               ACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCC                <  SRR4302540.584105/94‑1 (MQ=60)

                                                                               ACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCC                >  SRR4302540.4632156/1‑94 (MQ=60)

                                                                                CTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCT               >  SRR4302540.3187889/1‑94 (MQ=60)

                                                                                CTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCT               >  SRR4302540.6471619/1‑94 (MQ=60)

                                                                                CTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCT               >  SRR4302540.2180055/1‑94 (MQ=60)

                                                                                 TGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTAATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTG              >  SRR4302540.7458262/1‑94 (MQ=60)

                                                                                   ATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGCTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCT            >  SRR4302540.6558381/1‑94 (MQ=60)

                                                                                    TAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTC           <  SRR4302540.5370283/94‑1 (MQ=60)

                                                                                    TAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTC           <  SRR4302540.2686364/94‑1 (MQ=60)

                                                                                     AACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCA          >  SRR4302540.3164392/1‑94 (MQ=60)

                                                                                     AACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCA          >  SRR4302540.743636/1‑94 (MQ=60)

                                                                                     AACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCA          <  SRR4302540.8080680/94‑1 (MQ=60)

                                                                                      ACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCAC         >  SRR4302540.4063613/1‑94 (MQ=60)

                                                                                         CTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTAT      >  SRR4302540.1108118/1‑94 (MQ=60)

                                                                                         CTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTAT      <  SRR4302540.3410399/94‑1 (MQ=60)

                                                                                         CTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTAT      <  SRR4302540.6190291/94‑1 (MQ=60)

                                                                                           GGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGC    <  SRR4302540.6890898/94‑1 (MQ=60)

                                                                                           GGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGC    <  SRR4302540.9671123/94‑1 (MQ=60)

                                                                                             CGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAG  <  SRR4302540.4550049/94‑1 (MQ=60)

                                                                                             CGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAG  <  SRR4302540.5997849/94‑1 (MQ=60)

TATCGCTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAG  >  NC_000913/1845759‑1845945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`