Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,845,852	T→C	T61A (ACC→GCC)	selD ←	selenophosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,845,852	0	T	C	100.0%	565.2 / NA	176	T61A (ACC→GCC)	selD	selenophosphate synthase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (96/80); total (96/80)

Only 100 of 180 total aligned reads displayed.

GCTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTA  >  NC_000913/1845763‑1845940

   gATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCAtt                                                                                    <  1:1143051/93‑1 (MQ=255)

   gATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCAtt                                                                                    >  2:5864355/1‑93 (MQ=255)

    aTGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTg                                                                                   >  1:3556861/1‑93 (MQ=255)

     tGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGc                                                                                  >  1:2970525/1‑93 (MQ=255)

      ggCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGcc                                                                                 <  1:3441017/93‑1 (MQ=255)

        cGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCa                                                                               <  2:3136680/93‑1 (MQ=255)

         gTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAg                                                                              >  2:777680/1‑93 (MQ=255)

          ttAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGa                                                                             >  2:1534193/1‑93 (MQ=255)

           tAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGAt                                                                            <  1:6034166/93‑1 (MQ=255)

            aGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATc                                                                           >  1:2113614/1‑93 (MQ=255)

            aGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATc                                                                           <  1:2315227/93‑1 (MQ=255)

             gTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCg                                                                          >  1:1386701/1‑93 (MQ=255)

             gTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCg                                                                          <  2:2164913/93‑1 (MQ=255)

              tCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGt                                                                         >  2:3896226/1‑93 (MQ=255)

              tCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGt                                                                         >  1:5615840/1‑93 (MQ=255)

              tCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGt                                                                         >  2:3926373/1‑93 (MQ=255)

                gccgcAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTac                                                                       <  2:4148193/93‑1 (MQ=255)

                gccgcAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTac                                                                       <  2:1430561/93‑1 (MQ=255)

                 ccgcAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTaca                                                                      >  2:426189/1‑93 (MQ=255)

                 ccgcAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTaca                                                                      >  1:1855678/1‑93 (MQ=255)

                   gcAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACAcc                                                                    <  2:4373599/93‑1 (MQ=255)

                     aaTGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACAccgc                                                                  <  1:4045538/93‑1 (MQ=255)

                      aTGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACAccgcc                                                                 >  1:5891442/1‑93 (MQ=255)

                      aTGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACAccgcc                                                                 >  2:1110879/1‑93 (MQ=255)

                       tGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACAccgccg                                                                <  2:4514047/93‑1 (MQ=255)

                        gCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACcgccgc                                                               <  1:5099505/93‑1 (MQ=255)

                          ggCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCAt                                                             <  1:3884321/93‑1 (MQ=255)

                          ggCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCAt                                                             <  1:5569528/93‑1 (MQ=255)

                          ggCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCAt                                                             >  1:4542449/1‑93 (MQ=255)

                           gCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCAtc                                                            >  2:445516/1‑93 (MQ=255)

                             cAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCAtcgt                                                          >  1:491219/1‑93 (MQ=255)

                             cAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCAtcgt                                                          <  1:4526432/93‑1 (MQ=255)

                             cAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCAtcgt                                                          <  2:2968727/93‑1 (MQ=255)

                              aaaaTCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCAtcgtc                                                         <  1:4912492/93‑1 (MQ=255)

                              aaaaTCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCAtcgtc                                                         <  1:3937006/93‑1 (MQ=255)

                                  tCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCgg                                                     <  1:371807/93‑1 (MQ=255)

                                  tCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCgg                                                     >  1:5706664/1‑93 (MQ=255)

                                    gAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGt                                                   <  1:1671254/93‑1 (MQ=255)

                                    gAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGt                                                   <  2:613207/93‑1 (MQ=255)

                                      aaGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGttt                                                 >  1:4800312/1‑93 (MQ=255)

                                      aaGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGttt                                                 >  2:6043489/1‑93 (MQ=255)

                                       aGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTc                                                >  1:2600453/1‑93 (MQ=255)

                                        ggATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCa                                               <  1:81106/93‑1 (MQ=255)

                                         gATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCTTTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCAt                                              >  2:4093261/1‑93 (MQ=255)

                                         gATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCAt                                              <  1:73736/93‑1 (MQ=255)

                                           ttATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGGTTCATTa                                            >  2:368420/1‑93 (MQ=255)

                                           ttATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCAAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTa                                            <  2:5672313/93‑1 (MQ=255)

                                            tATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTAc                                           <  2:2188735/93‑1 (MQ=255)

                                            tATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTAc                                           <  1:3802628/93‑1 (MQ=255)

                                             aTCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTAcc                                          >  1:3469669/1‑93 (MQ=255)

                                              tCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTAccc                                         >  2:127636/1‑93 (MQ=255)

                                               cAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCca                                        <  1:2946945/93‑1 (MQ=255)

                                                aaCGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGGCCAGATCGTACACCGCCGCGTCGTCGCGGGTTTCATTACCcac                                       <  2:1836458/93‑1 (MQ=255)

                                                aaCGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCcac                                       >  1:2062023/1‑93 (MQ=255)

                                                 aCGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCcaca                                      <  1:5694106/93‑1 (MQ=255)

                                                  cGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACaa                                     <  1:3132363/93‑1 (MQ=255)

                                                  cGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACaa                                     <  2:4492126/93‑1 (MQ=255)

                                                  cGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACaa                                     <  2:3260584/93‑1 (MQ=255)

                                                  cGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACaa                                     <  2:681251/93‑1 (MQ=255)

                                                   gATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAg                                    <  2:965277/93‑1 (MQ=255)

                                                   gATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAg                                    >  2:5289497/1‑93 (MQ=255)

                                                   gATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAg                                    >  2:6223520/1‑93 (MQ=255)

                                                     tCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCa                                  >  1:5830150/1‑93 (MQ=255)

                                                     tCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCa                                  >  2:3473279/1‑93 (MQ=255)

                                                       ggCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCaaa                                <  2:5535604/93‑1 (MQ=255)

                                                        gCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAAt                               >  1:5860595/1‑93 (MQ=255)

                                                        gCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAAt                               <  2:1210121/93‑1 (MQ=255)

                                                         cATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAAtt                              <  2:25626/93‑1 (MQ=255)

                                                          aTAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTc                             <  2:2934935/93‑1 (MQ=255)

                                                          aTAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTc                             >  2:3395510/1‑93 (MQ=255)

                                                          aTAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTc                             <  2:36145/93‑1 (MQ=255)

                                                          aTAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTc                             >  1:4726379/1‑93 (MQ=255)

                                                           tAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCg                            >  1:1764773/1‑93 (MQ=255)

                                                           tAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCg                            >  2:5626241/1‑93 (MQ=255)

                                                            aaaGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCgg                           >  2:2333005/1‑93 (MQ=255)

                                                              agaagTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGAt                         <  2:918623/93‑1 (MQ=255)

                                                              agaagTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGAt                         >  1:1206931/1‑93 (MQ=255)

                                                              agaagTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGAt                         >  1:1638884/1‑93 (MQ=255)

                                                              agaagTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGAt                         <  2:5082699/93‑1 (MQ=255)

                                                               gaagTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATc                        <  1:3312201/93‑1 (MQ=255)

                                                               gaagTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATc                        <  1:5980800/93‑1 (MQ=255)

                                                               gaagTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATc                        <  1:6089783/93‑1 (MQ=255)

                                                               gaagTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATc                        >  1:6196130/1‑93 (MQ=255)

                                                               gaagTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATc                        >  2:5159846/1‑93 (MQ=255)

                                                               gaagTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATc                        <  2:4537411/93‑1 (MQ=255)

                                                                     ggtggtACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACaaa                  <  2:4651049/93‑1 (MQ=255)

                                                                     ggtggtACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACaaa                  <  2:1693144/93‑1 (MQ=255)

                                                                      gtggtACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAAc                 >  1:6276307/1‑93 (MQ=255)

                                                                       tggtACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACt                <  1:5354107/93‑1 (MQ=255)

                                                                        ggtACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACtt               <  1:2006296/93‑1 (MQ=255)

                                                                        ggaaCTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACtt               >  1:112605/4‑93 (MQ=255)

                                                                         gtACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTc              <  1:4358627/93‑1 (MQ=255)

                                                                         gtACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTc              >  2:631486/1‑93 (MQ=255)

                                                                           aCTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGc            <  1:4311187/93‑1 (MQ=255)

                                                                            cTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGcc           <  2:4988396/93‑1 (MQ=255)

                                                                              gATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACCAACTTCGCCTg         >  2:2758822/1‑93 (MQ=255)

                                                                                tAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCt       >  1:1628904/1‑93 (MQ=255)

                                                                                tAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCt       >  1:3577450/1‑93 (MQ=255)

                                                                                 aaCGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTc      >  2:2049428/1‑93 (MQ=255)

                                                                                     cTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTa  <  1:1709214/93‑1 (MQ=255)

GCTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTA  >  NC_000913/1845763‑1845940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TATCGCTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAG  >  NC_000913/1845759‑1845945

TATCGCTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGC                                                                                               >  SRR4302533.1117053/1‑94 (MQ=60)

TATCGCTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGC                                                                                               <  SRR4302533.3169628/94‑1 (MQ=60)

  TCGCTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGC                                                                                             <  SRR4302533.5528848/94‑1 (MQ=60)

   CGCTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCC                                                                                            >  SRR4302533.1268359/1‑94 (MQ=60)

      TGATGGGTTTAGTCGCCGCAATGCGGCCAAAAGCGAAAGGATTATCACCGATCGGAATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATT                                                                                         <  SRR4302533.4649701/94‑1 (MQ=60)

       GATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTG                                                                                        <  SRR4302533.1149865/94‑1 (MQ=60)

       GATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTG                                                                                        >  SRR4302533.3578781/1‑94 (MQ=60)

       GATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTG                                                                                        <  SRR4302533.1729937/94‑1 (MQ=60)

        ATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGC                                                                                       >  SRR4302533.277901/1‑94 (MQ=60)

        ATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGC                                                                                       >  SRR4302533.2988688/1‑94 (MQ=60)

          GGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCC                                                                                     <  SRR4302533.3462264/94‑1 (MQ=60)

          GGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCC                                                                                     >  SRR4302533.4767447/1‑94 (MQ=60)

           GCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCA                                                                                    <  SRR4302533.4622675/94‑1 (MQ=60)

            CGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAG                                                                                   >  SRR4302533.6264371/1‑94 (MQ=60)

              TTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGAT                                                                                 <  SRR4302533.3861600/94‑1 (MQ=60)

              GTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGAT                                                                                 <  SRR4302533.590202/94‑1 (MQ=60)

               TAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATC                                                                                <  SRR4302533.6080342/94‑1 (MQ=60)

               TAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATC                                                                                <  SRR4302533.6070732/94‑1 (MQ=60)

               TAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATC                                                                                >  SRR4302533.2126648/1‑94 (MQ=60)

                AGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCG                                                                               >  SRR4302533.1640675/1‑94 (MQ=60)

                AGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCG                                                                               >  SRR4302533.1395047/1‑94 (MQ=60)

                AGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCG                                                                               <  SRR4302533.2329566/94‑1 (MQ=60)

                 GTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGT                                                                              >  SRR4302533.5649787/1‑94 (MQ=60)

                    GCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACA                                                                           >  SRR4302533.1866995/1‑94 (MQ=60)

                      CGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACC                                                                         >  SRR4302533.1795861/1‑94 (MQ=60)

                      CGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACC                                                                         >  SRR4302533.3809128/1‑94 (MQ=60)

                       GCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCG                                                                        <  SRR4302533.53239/94‑1 (MQ=60)

                         AATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCC                                                                      <  SRR4302533.4070158/94‑1 (MQ=60)

                         AATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCC                                                                      <  SRR4302533.3591872/94‑1 (MQ=60)

                         AATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCC                                                                      >  SRR4302533.5927029/1‑94 (MQ=60)

                          ATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCG                                                                     >  SRR4302533.1192570/1‑94 (MQ=60)

                            GCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCA                                                                   <  SRR4302533.5130313/94‑1 (MQ=60)

                             CGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCAT                                                                  >  SRR4302533.4569956/1‑94 (MQ=60)

                              GGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATC                                                                 <  SRR4302533.5603193/94‑1 (MQ=60)

                              GGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATC                                                                 >  SRR4302533.3666184/1‑94 (MQ=60)

                              GGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATC                                                                 <  SRR4302533.3908021/94‑1 (MQ=60)

                               GCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCG                                                                >  SRR4302533.1610831/1‑94 (MQ=60)

                                CCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGT                                                               >  SRR4302533.494155/1‑94 (MQ=60)

                                 CAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTC                                                              <  SRR4302533.4553837/94‑1 (MQ=60)

                                  AAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCG                                                             <  SRR4302533.4942251/94‑1 (MQ=60)

                                  AAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCG                                                             <  SRR4302533.3961038/94‑1 (MQ=60)

                                   AAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGC                                                            <  SRR4302533.1694670/94‑1 (MQ=60)

                                   AAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGC                                                            <  SRR4302533.4352359/94‑1 (MQ=60)

                                     atcgaaatagTTATCAACAATCGGCCAAAAGAAATCGGTGGTACTAATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGG                                                          <  SRR4302533.5717196/84‑1 (MQ=60)

                                     ATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGG                                                          <  SRR4302533.4425076/94‑1 (MQ=60)

                                     ATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGG                                                          >  SRR4302533.5741134/1‑94 (MQ=60)

                                      TCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGG                                                         <  SRR4302533.5855242/94‑1 (MQ=60)

                                      TCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGG                                                         <  SRR4302533.374050/94‑1 (MQ=60)

                                        GAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTT                                                       <  SRR4302533.1681355/94‑1 (MQ=60)

                                        GAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGGT                                                       >  SRR4302533.2346178/1‑94 (MQ=60)

                                         AAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTT                                                      >  SRR4302533.4829388/1‑94 (MQ=60)

                                         AAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTT                                                      >  SRR4302533.5069824/1‑94 (MQ=60)

                                         AAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTT                                                      >  SRR4302533.4072635/1‑94 (MQ=60)

                                          AAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTC                                                     >  SRR4302533.2616431/1‑94 (MQ=60)

                                            GGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCAT                                                   <  SRR4302533.81621/94‑1 (MQ=60)

                                             GATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATT                                                  <  SRR4302533.74211/94‑1 (MQ=60)

                                               TTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTAC                                                <  SRR4302533.315675/94‑1 (MQ=60)

                                                TATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACC                                               <  SRR4302533.3825897/94‑1 (MQ=60)

                                                TATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACC                                               >  SRR4302533.3491087/1‑94 (MQ=60)

                                                   CAGCGATCGCCATAAAGAAGTCGCTGTCACTGATAACGCTGGCGCCATCGCCCAGACCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCAC                                            <  SRR4302533.1298875/94‑1 (MQ=60)

                                                   CAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCAC                                            >  SRR4302533.93465/1‑94 (MQ=60)

                                                   CAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCAC                                            >  SRR4302533.2074740/1‑94 (MQ=60)

                                                   CAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCAC                                            <  SRR4302533.2964967/94‑1 (MQ=60)

                                                     ACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAA                                          <  SRR4302533.5728489/94‑1 (MQ=60)

                                                      CGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAG                                         <  SRR4302533.5893514/94‑1 (MQ=60)

                                                      CGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAG                                         <  SRR4302533.3151622/94‑1 (MQ=60)

                                                       GATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGC                                        >  SRR4302533.3464393/1‑94 (MQ=60)

                                                        ATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCA                                       >  SRR4302533.5865382/1‑94 (MQ=60)

                                                        ATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCA                                       >  SRR4302533.2123249/1‑94 (MQ=60)

                                                          CGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAA                                     <  SRR4302533.306294/94‑1 (MQ=60)

                                                           GGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAAT                                    >  SRR4302533.5896025/1‑94 (MQ=60)

                                                             CATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTC                                  >  SRR4302533.614686/1‑94 (MQ=60)

                                                             CATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTC                                  >  SRR4302533.4755034/1‑94 (MQ=60)

                                                              ATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCG                                 >  SRR4302533.1775519/1‑94 (MQ=60)

                                                                 AAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGAT                              >  SRR4302533.1214178/1‑94 (MQ=60)

                                                                 AAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGAT                              >  SRR4302533.1648807/1‑94 (MQ=60)

                                                                  AGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATC                             >  SRR4302533.6233303/1‑94 (MQ=60)

                                                                   GAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCA                            <  SRR4302533.3332656/94‑1 (MQ=60)

                                                                   GAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCA                            <  SRR4302533.6017024/94‑1 (MQ=60)

                                                                   GAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCA                            <  SRR4302533.6126593/94‑1 (MQ=60)

                                                                      GTCGGTGGTANTGATAACGCTGGCGCCATTGCCCAGATCGTACANCNCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACA                         >  SRR4302533.6012408/1‑94 (MQ=60)

                                                                        CGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAA                       >  SRR4302533.3402524/1‑94 (MQ=60)

                                                                        CGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAA                       <  SRR4302533.2176763/94‑1 (MQ=60)

                                                                         GGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAAC                      >  SRR4302533.564774/1‑94 (MQ=60)

                                                                         GGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAAC                      >  SRR4302533.669827/1‑94 (MQ=60)

                                                                         GGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAAC                      >  SRR4302533.6313786/1‑94 (MQ=60)

                                                                           TGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTT                    <  SRR4302533.5386243/94‑1 (MQ=60)

                                                                           TGGAACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTT                    >  SRR4302533.113317/1‑94 (MQ=60)

                                                                            GGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTC                   <  SRR4302533.2018652/94‑1 (MQ=60)

                                                                             GTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCG                  <  SRR4302533.4024441/94‑1 (MQ=60)

                                                                             GTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCG                  <  SRR4302533.4384934/94‑1 (MQ=60)

                                                                               ACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCC                <  SRR4302533.4337140/94‑1 (MQ=60)

                                                                                   ATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCT            >  SRR4302533.5526599/1‑94 (MQ=60)

                                                                                   ATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCT            >  SRR4302533.3599495/1‑94 (MQ=60)

                                                                                   ATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCT            >  SRR4302533.1638769/1‑94 (MQ=60)

                                                                                     AACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCA          >  SRR4302533.4895277/1‑94 (MQ=60)

                                                                                         CTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTAT      <  SRR4302533.1890865/94‑1 (MQ=60)

                                                                                         CTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTAT      <  SRR4302533.1719571/94‑1 (MQ=60)

                                                                                          TGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATG     >  SRR4302533.2792805/1‑94 (MQ=60)

                                                                                           GGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGC    <  SRR4302533.4600967/94‑1 (MQ=60)

                                                                                             CGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAG  <  SRR4302533.2497837/94‑1 (MQ=60)

                                                                                             CGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAG  <  SRR4302533.5041074/94‑1 (MQ=60)

                                                                                             CGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAG  >  SRR4302533.134606/1‑94 (MQ=60)

TATCGCTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAG  >  NC_000913/1845759‑1845945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`