Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,858,325	A→G	D344D (GAT→GAC)	b1776 ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,858,325	0	A	G	99.7%	955.0 / ‑5.3	299	D344D (GAT→GAC)	b1776	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (141/157); total (142/157)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.58e-01

Only 100 of 310 total aligned reads displayed.

GTTTTGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAA  >  NC_000913/1858235‑1858414

   ttGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGAc                                                                                      <  2:9875025/93‑1 (MQ=255)

   ttGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGAc                                                                                      >  2:3188157/1‑93 (MQ=255)

    tGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGAcc                                                                                     <  1:540909/93‑1 (MQ=255)

     gTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCa                                                                                    <  1:8766836/93‑1 (MQ=255)

     gTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCa                                                                                    >  1:2175286/1‑93 (MQ=255)

      ttATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCAt                                                                                   >  1:2675502/1‑93 (MQ=255)

       tATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATc                                                                                  >  1:7065617/1‑93 (MQ=255)

       tATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATc                                                                                  <  2:2962916/93‑1 (MQ=255)

       tATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATc                                                                                  >  1:4380616/1‑93 (MQ=255)

       tATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATc                                                                                  >  1:9816164/1‑93 (MQ=255)

        aTCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCg                                                                                 >  1:10000159/1‑93 (MQ=255)

         tCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGc                                                                                <  1:7386983/93‑1 (MQ=255)

         tCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGc                                                                                <  1:1648864/93‑1 (MQ=255)

           tGTTCCATGCGATAATGTTCCTTCAGGAGAGAGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCAt                                                                              >  2:7572820/1‑93 (MQ=255)

           tGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCAt                                                                              >  1:7815473/1‑93 (MQ=255)

            gTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATc                                                                             >  1:4699942/1‑93 (MQ=255)

              tCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCaa                                                                           <  2:7167050/93‑1 (MQ=255)

                 aTGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAAcc                                                                        >  1:756085/1‑93 (MQ=255)

                 aTGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAAcc                                                                        <  2:5052291/93‑1 (MQ=255)

                  tGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAAccc                                                                       >  1:5682193/1‑93 (MQ=255)

                   gCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCt                                                                      <  2:6863718/93‑1 (MQ=255)

                    cGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCtt                                                                     <  1:1210828/93‑1 (MQ=255)

                    cGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCtt                                                                     >  2:2659294/1‑93 (MQ=255)

                     gATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTc                                                                    >  2:5285250/1‑93 (MQ=255)

                      aTAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCTACCATCGCATCAAACCCTTcg                                                                   >  1:7924456/1‑93 (MQ=255)

                      aTAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTcg                                                                   >  2:5916004/1‑93 (MQ=255)

                      aTAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTcg                                                                   >  2:4855217/1‑93 (MQ=255)

                      aTAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTcg                                                                   >  1:3274679/1‑93 (MQ=255)

                       tAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTcgc                                                                  >  2:3920769/1‑93 (MQ=255)

                       tAACGTTCCTTCAGGAGAGTGGGTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTcgc                                                                  >  2:7316550/1‑93 (MQ=255)

                           gTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCa                                                              <  1:9632779/93‑1 (MQ=255)

                           gTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCa                                                              >  2:1709989/1‑93 (MQ=255)

                            ttCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCAt                                                             <  2:4223251/93‑1 (MQ=255)

                            ttCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCAt                                                             >  1:8453375/1‑93 (MQ=255)

                            tgtcTTCAGTAGAGTGTTTAATCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCAt                                                             <  1:7722150/90‑1 (MQ=255)

                             tCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCAtt                                                            <  1:2878314/93‑1 (MQ=255)

                             tCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCAtt                                                            >  2:2530898/1‑93 (MQ=255)

                             tCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCAtt                                                            >  1:8349154/1‑93 (MQ=255)

                             tCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGCTTTGTCGACCATCGCATCAAACCCTTCGCGCCAtt                                                            >  2:2770906/1‑93 (MQ=255)

                              ccTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTg                                                           >  2:5729165/1‑93 (MQ=255)

                                  cAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGAc                                                       <  2:6784499/93‑1 (MQ=255)

                                  cAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGAc                                                       >  1:7642577/1‑93 (MQ=255)

                                   aGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACa                                                      <  2:8539433/93‑1 (MQ=255)

                                   aGGAGAGTGCTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACGATCGCATCAAACCCTTCGCGCCATTTCGACa                                                      <  2:799337/93‑1 (MQ=255)

                                     gagaGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAgg                                                    >  2:3339018/1‑93 (MQ=255)

                                     gagaGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAgg                                                    <  2:3553907/93‑1 (MQ=255)

                                     gagaGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAgg                                                    >  2:8078866/1‑93 (MQ=255)

                                      ggagTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGc                                                   <  1:9445892/92‑1 (MQ=255)

                                      agagTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGc                                                   >  1:3457193/1‑93 (MQ=255)

                                      agagTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGc                                                   >  2:4821543/1‑93 (MQ=255)

                                      agagTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGc                                                   <  1:5057493/93‑1 (MQ=255)

                                       gagTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGcc                                                  >  1:7156641/1‑93 (MQ=255)

                                          tGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGAt                                               >  1:9423903/1‑93 (MQ=255)

                                           ggTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATa                                              >  1:1676733/1‑93 (MQ=255)

                                             ttATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACg                                            <  2:9665600/93‑1 (MQ=255)

                                              tATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGa                                           <  2:748688/93‑1 (MQ=255)

                                              tATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGa                                           <  1:3070988/93‑1 (MQ=255)

                                              tATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGa                                           >  1:2240610/1‑93 (MQ=255)

                                              tATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGa                                           <  1:1936052/93‑1 (MQ=255)

                                               aTTCATCAAATTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCAACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGAt                                          <  2:9520962/93‑1 (MQ=255)

                                                  catcaAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCg                                       >  1:8692708/1‑93 (MQ=255)

                                                  catcaAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCg                                       <  1:2945500/93‑1 (MQ=255)

                                                   atcaAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGt                                      <  1:1085140/93‑1 (MQ=255)

                                                   atcaAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGt                                      <  2:937807/93‑1 (MQ=255)

                                                   atcaAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGt                                      >  2:6033394/1‑93 (MQ=255)

                                                    tcaAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTg                                     >  2:9685027/1‑93 (MQ=255)

                                                     caAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGa                                    >  1:1545227/1‑93 (MQ=255)

                                                     caAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGa                                    <  1:3925016/93‑1 (MQ=255)

                                                      aaaGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAACCCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGat                                   <  2:4073280/93‑1 (MQ=255)

                                                      aaaGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGat                                   >  2:8180657/1‑93 (MQ=255)

                                                      aaaGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGat                                   >  1:7093772/1‑93 (MQ=255)

                                                      aaaGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGat                                   <  1:9578815/93‑1 (MQ=255)

                                                      aaaGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTAat                                   <  1:8931315/93‑1 (MQ=255)

                                                       aaGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGatc                                  <  2:3671692/93‑1 (MQ=255)

                                                       aaGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGatc                                  <  1:2098502/93‑1 (MQ=255)

                                                         gTCGTAAGTCATGATCGCTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGatcat                                <  1:6351399/93‑1 (MQ=255)

                                                          tCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGatcatc                               >  2:6410916/1‑93 (MQ=255)

                                                              aaGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGtt                           <  1:1791098/93‑1 (MQ=255)

                                                               aGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGttt                          <  2:7844491/93‑1 (MQ=255)

                                                                   aTGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACt                      <  1:6101010/93‑1 (MQ=255)

                                                                     gATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACttt                    <  2:2614212/93‑1 (MQ=255)

                                                                      aTCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTg                   >  2:9327842/1‑93 (MQ=255)

                                                                       tCACTTTGCTGGCGGTTTTGTCGACCATCACATCAACCCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCTTCGGTTTGACTTTGa                  <  2:1004573/93‑1 (MQ=255)

                                                                       tCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGa                  >  2:1141831/1‑93 (MQ=255)

                                                                       tCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGa                  >  2:1948278/1‑93 (MQ=255)

                                                                       tCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGa                  <  1:2773041/93‑1 (MQ=255)

                                                                        cACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGAt                 >  2:4000645/1‑93 (MQ=255)

                                                                        cACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGAt                 <  2:8739374/93‑1 (MQ=255)

                                                                          cTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAg               >  1:5165519/1‑93 (MQ=255)

                                                                          cTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAg               <  1:373179/93‑1 (MQ=255)

                                                                           tttGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAgc              <  1:8618143/93‑1 (MQ=255)

                                                                           tttGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAgc              >  2:8399456/1‑93 (MQ=255)

                                                                           tttGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAgc              >  1:8548638/1‑93 (MQ=255)

                                                                            ttGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAgcg             >  2:9287585/1‑93 (MQ=255)

                                                                              gATTGCGGTTTTGTCGACCATCGCATCAACCCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGcgcc           <  1:6908956/93‑1 (MQ=255)

                                                                              gATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGcgcc           >  1:9385076/1‑93 (MQ=255)

                                                                              gATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGcgcc           <  1:4968968/93‑1 (MQ=255)

                                                                                 tGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCt        <  1:5775172/93‑1 (MQ=255)

                                                                                  gCGGTTTTGTCGCCCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTg       <  2:4641448/93‑1 (MQ=255)

                                                                                    ggTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGc     >  2:135544/1‑93 (MQ=255)

GTTTTGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAA  >  NC_000913/1858235‑1858414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGTTTTGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAATAG  >  NC_000913/1858234‑1858417

GGTTTTGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTC                                                                                            >  SRR4302540.3460510/1‑94 (MQ=60)

 GTTTTGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCG                                                                                           >  SRR4302540.1067997/1‑94 (MQ=60)

 GTTTTGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCG                                                                                           >  SRR4302540.8454065/1‑94 (MQ=60)

   TTTGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGAC                                                                                         >  SRR4302540.9537539/1‑94 (MQ=60)

    TTGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACC                                                                                        <  SRR4302540.7817654/94‑1 (MQ=60)

    TTGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACC                                                                                        <  SRR4302540.165845/94‑1 (MQ=60)

    TTGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACC                                                                                        <  SRR4302540.575575/94‑1 (MQ=60)

     TGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCA                                                                                       <  SRR4302540.8723534/94‑1 (MQ=60)

     TGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCA                                                                                       >  SRR4302540.2198744/1‑94 (MQ=60)

     TGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCA                                                                                       >  SRR4302540.168911/1‑94 (MQ=60)

     TGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCA                                                                                       <  SRR4302540.546765/94‑1 (MQ=60)

      TTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCAT                                                                                      <  SRR4302540.6529010/94‑1 (MQ=60)

      GTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCAT                                                                                      <  SRR4302540.8868454/94‑1 (MQ=60)

      GTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCAT                                                                                      >  SRR4302540.2704465/1‑94 (MQ=60)

       TTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATC                                                                                     >  SRR4302540.9931023/1‑94 (MQ=60)

       TTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATC                                                                                     >  SRR4302540.7144195/1‑94 (MQ=60)

       TTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATC                                                                                     >  SRR4302540.4428181/1‑94 (MQ=60)

       TTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATC                                                                                     >  SRR4302540.4428180/1‑94 (MQ=60)

        TATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCG                                                                                    >  SRR4302540.2819794/1‑94 (MQ=60)

        TATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCG                                                                                    >  SRR4302540.10116663/1‑94 (MQ=60)

        TATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCG                                                                                    <  SRR4302540.9446916/94‑1 (MQ=60)

         ATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGC                                                                                   >  SRR4302540.1141805/1‑94 (MQ=60)

          TCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCA                                                                                  <  SRR4302540.1666854/94‑1 (MQ=60)

          TCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCA                                                                                  <  SRR4302540.1666859/94‑1 (MQ=60)

          TCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCA                                                                                  <  SRR4302540.7469290/94‑1 (MQ=60)

          TCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCA                                                                                  <  SRR4302540.8400626/94‑1 (MQ=60)

          TCTGTTCCATGCGATAACGCTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCA                                                                                  >  SRR4302540.3590645/1‑94 (MQ=60)

           CTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCAT                                                                                 >  SRR4302540.7906806/1‑94 (MQ=60)

           CTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCAT                                                                                 <  SRR4302540.955584/94‑1 (MQ=60)

            TGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATC                                                                                >  SRR4302540.4750179/1‑94 (MQ=60)

             GTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCA                                                                               <  SRR4302540.7819107/94‑1 (MQ=60)

              TTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAA                                                                              >  SRR4302540.3532346/1‑94 (MQ=60)

                CCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAAC                                                                            >  SRR4302540.7737567/1‑94 (MQ=60)

                 CATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACC                                                                           >  SRR4302540.764141/1‑94 (MQ=60)

                 CATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACC                                                                           >  SRR4302540.7161475/1‑94 (MQ=60)

                 CATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACC                                                                           <  SRR4302540.7107635/94‑1 (MQ=60)

                 CATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACC                                                                           <  SRR4302540.764008/94‑1 (MQ=60)

                  CTGCGATAACGTTCCTTCATGAGAGTGTTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCC                                                                          <  SRR4302540.3435539/94‑1 (MQ=60)

                  ATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCC                                                                          <  SRR4302540.2226242/94‑1 (MQ=60)

                  ATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCC                                                                          >  SRR4302540.2268288/1‑94 (MQ=60)

                  ATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCC                                                                          >  SRR4302540.5744753/1‑94 (MQ=60)

                   TGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCT                                                                         <  SRR4302540.5048793/94‑1 (MQ=60)

                    GCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTT                                                                        <  SRR4302540.7691412/94‑1 (MQ=60)

                     CGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTC                                                                       <  SRR4302540.2968204/94‑1 (MQ=60)

                     CGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTC                                                                       <  SRR4302540.1223240/94‑1 (MQ=60)

                      GATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGTGGTTTTGTCGACCATCGCATCAAACCCTTCG                                                                      >  SRR4302540.165443/1‑94 (MQ=60)

                      GATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCTACCATCGCATCAAACCCTTCG                                                                      >  SRR4302540.8016822/1‑94 (MQ=60)

                      GATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCG                                                                      >  SRR4302540.9240258/1‑94 (MQ=60)

                      GATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCG                                                                      >  SRR4302540.3310431/1‑94 (MQ=60)

                       ATAACGTTCCTTCAGGAGAGTGGTTCTTTCTCAAAGTCGTAAGTCATGATCACTTTGATTCCGGTTTTGTCGCCCATCGCATCCAACCCTTCGC                                                                     >  SRR4302540.5578148/1‑94 (MQ=60)

                        TAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCG                                                                    >  SRR4302540.7171035/1‑94 (MQ=60)

                          ACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCC                                                                  <  SRR4302540.3218448/94‑1 (MQ=60)

                            GTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCAT                                                                <  SRR4302540.9746279/94‑1 (MQ=60)

                            GTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCAT                                                                <  SRR4302540.9746280/94‑1 (MQ=60)

                            GTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCAT                                                                >  SRR4302540.8551518/1‑94 (MQ=60)

                            GTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCAT                                                                <  SRR4302540.2254931/94‑1 (MQ=60)

                            GTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCAT                                                                >  SRR4302540.4236480/1‑94 (MQ=60)

                            GTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTATTGTCGACCATCGCATCAAACCCTTCGCGCCAT                                                                >  SRR4302540.9799260/1‑94 (MQ=60)

                             tgtCTTCAGTAGAGTGTTTAATCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATT                                                               <  SRR4302540.7811883/91‑1 (MQ=60)

                             TTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATT                                                               >  SRR4302540.8446055/1‑94 (MQ=60)

                             TTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATT                                                               >  SRR4302540.1870580/1‑94 (MQ=60)

                             TTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATT                                                               <  SRR4302540.7811882/94‑1 (MQ=60)

                              TCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTG                                                              <  SRR4302540.2909615/94‑1 (MQ=60)

                                  TCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGAC                                                          >  SRR4302540.7728591/1‑94 (MQ=60)

                                   CAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACA                                                         <  SRR4302540.7197715/94‑1 (MQ=60)

                                    AGGATAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAG                                                        <  SRR4302540.8586069/94‑1 (MQ=60)

                                    AGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAG                                                        <  SRR4302540.5883631/94‑1 (MQ=60)

                                    AGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAG                                                        <  SRR4302540.894327/94‑1 (MQ=60)

                                    AGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAG                                                        <  SRR4302540.4560783/94‑1 (MQ=60)

                                     GGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGG                                                       <  SRR4302540.958906/94‑1 (MQ=60)

                                      GAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGC                                                      >  SRR4302540.3494825/1‑94 (MQ=60)

                                       GGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCC                                                     <  SRR4302540.9558027/94‑1 (MQ=60)

                                       AGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCC                                                     >  SRR4302540.5814477/1‑94 (MQ=60)

                                       AGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCC                                                     <  SRR4302540.4972131/94‑1 (MQ=60)

                                       AGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCC                                                     <  SRR4302540.5112170/94‑1 (MQ=60)

                                       AGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCC                                                     >  SRR4302540.7236406/1‑94 (MQ=60)

                                       AGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCC                                                     <  SRR4302540.7273840/94‑1 (MQ=60)

                                         AGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGA                                                   >  SRR4302540.10005139/1‑94 (MQ=60)

                                          GTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGAT                                                  >  SRR4302540.9535892/1‑94 (MQ=60)

                                          GTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGAT                                                  >  SRR4302540.1476175/1‑94 (MQ=60)

                                          GTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGAT                                                  >  SRR4302540.1035715/1‑94 (MQ=60)

                                          GTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGAT                                                  >  SRR4302540.8136993/1‑94 (MQ=60)

                                           TGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATA                                                 >  SRR4302540.6114985/1‑94 (MQ=60)

                                           TGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATA                                                 <  SRR4302540.8584548/94‑1 (MQ=60)

                                           TGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATA                                                 >  SRR4302540.1694947/1‑94 (MQ=60)

                                            GGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAG                                                >  SRR4302540.7934789/1‑94 (MQ=60)

                                            GGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAC                                                >  SRR4302540.526863/1‑94 (MQ=60)

                                             GTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACG                                               <  SRR4302540.28018/94‑1 (MQ=60)

                                             GTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACG                                               >  SRR4302540.8512346/1‑94 (MQ=60)

                                             GTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACG                                               <  SRR4302540.2230791/94‑1 (MQ=60)

                                              TTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGA                                              >  SRR4302540.2264693/1‑94 (MQ=60)

                                               TATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGAT                                             <  SRR4302540.5136580/94‑1 (MQ=60)

                                               TATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGAT                                             <  SRR4302540.1957143/94‑1 (MQ=60)

                                               TATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGAT                                             <  SRR4302540.3007560/94‑1 (MQ=60)

                                               TATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGAT                                             <  SRR4302540.1151972/94‑1 (MQ=60)

                                               TATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGAT                                             <  SRR4302540.3104685/94‑1 (MQ=60)

                                                ATTCATCAAAGTCGTAAGTCATGATCGCTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATG                                            <  SRR4302540.4170665/94‑1 (MQ=60)

                                                ATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGACGATACGATG                                            >  SRR4302540.3203106/1‑94 (MQ=60)

                                                  TCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCG                                          >  SRR4302540.6305786/1‑94 (MQ=60)

                                                  TCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCG                                          >  SRR4302540.8793628/1‑94 (MQ=60)

                                                   CATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGT                                         <  SRR4302540.2977891/94‑1 (MQ=60)

                                                    ATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTG                                        >  SRR4302540.745319/1‑94 (MQ=60)

                                                    ATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTG                                        <  SRR4302540.7082112/94‑1 (MQ=60)

                                                    ATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTG                                        <  SRR4302540.1096266/94‑1 (MQ=60)

                                                    ATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTG                                        >  SRR4302540.212056/1‑94 (MQ=60)

                                                     TCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGA                                       <  SRR4302540.8327851/94‑1 (MQ=60)

                                                     TCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGA                                       >  SRR4302540.1562283/1‑94 (MQ=60)

                                                      CAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGAT                                      <  SRR4302540.5651830/94‑1 (MQ=60)

                                                      CAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGAT                                      >  SRR4302540.7172713/1‑94 (MQ=60)

                                                      CAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGAT                                      >  SRR4302540.10019107/1‑94 (MQ=60)

                                                      CAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGAT                                      <  SRR4302540.3967487/94‑1 (MQ=60)

                                                       AAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATC                                     >  SRR4302540.9524780/1‑94 (MQ=60)

                                                       AAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATC                                     <  SRR4302540.9691937/94‑1 (MQ=60)

                                                       AAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTAATC                                     <  SRR4302540.9035558/94‑1 (MQ=60)

                                                        AAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCA                                    <  SRR4302540.2121033/94‑1 (MQ=60)

                                                         AGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCAT                                   <  SRR4302540.8823570/94‑1 (MQ=60)

                                                          GTCGTAAGTCATGATCGCTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATC                                  <  SRR4302540.6422131/94‑1 (MQ=60)

                                                          GTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATC                                  >  SRR4302540.3774965/1‑94 (MQ=60)

                                                          GTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATC                                  <  SRR4302540.5143580/94‑1 (MQ=60)

                                                          GTCGGAAGTCATGATCGCTTTGATTGCGGTTTTGGCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATC                                  <  SRR4302540.5030180/94‑1 (MQ=60)

                                                            CGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGG                                >  SRR4302540.2500132/1‑94 (MQ=60)

                                                            CGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGG                                <  SRR4302540.290755/94‑1 (MQ=60)

                                                              TAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTT                              >  SRR4302540.718092/1‑94 (MQ=60)

                                                              TAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTT                              <  SRR4302540.8832229/94‑1 (MQ=60)

                                                               AAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTT                             <  SRR4302540.1810563/94‑1 (MQ=60)

                                                                AGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTG                            >  SRR4302540.1272086/1‑94 (MQ=60)

                                                                AGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTG                            <  SRR4302540.9639249/94‑1 (MQ=60)

                                                                AGGCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCGTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGtgttg                            >  SRR4302540.1272085/1‑89 (MQ=60)

                                                                 GTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGA                           <  SRR4302540.9760072/94‑1 (MQ=60)

                                                                  TCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGAC                          >  SRR4302540.1842737/1‑94 (MQ=60)

                                                                   CATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACT                         >  SRR4302540.1711040/1‑94 (MQ=60)

                                                                   CATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACT                         <  SRR4302540.2705383/94‑1 (MQ=60)

                                                                    ATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTT                        >  SRR4302540.6204533/1‑94 (MQ=60)

                                                                    ATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTT                        <  SRR4302540.6168939/94‑1 (MQ=60)

                                                                    ATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACACTTCGCGCCATTGCGACAGGCCGATACGCTGCGAGATCATCGGCTTGACTT                        >  SRR4302540.4993966/1‑94 (MQ=60)

                                                                     tgagctcttggagttCGGTTTTGTCGACAATCGCATCAATCCCGTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTT                       <  SRR4302540.438106/79‑1 (MQ=60)

                                                                       gtgACTTTGATTGCGTTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGA                     <  SRR4302540.77083/91‑1 (MQ=60)

                                                                        TCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGAT                    <  SRR4302540.2803004/94‑1 (MQ=60)

                                                                        TCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGAT                    <  SRR4302540.2803003/94‑1 (MQ=60)

                                                                        TCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGAT                    <  SRR4302540.4265421/94‑1 (MQ=60)

                                                                         CACTTTGATTGCGGTTTTGTCNANCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATA                   <  SRR4302540.2192598/94‑1 (MQ=60)

                                                                         CACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATA                   <  SRR4302540.3786956/94‑1 (MQ=60)

                                                                         CACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATA                   <  SRR4302540.8914617/94‑1 (MQ=60)

                                                                          ACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAG                  >  SRR4302540.6829080/1‑94 (MQ=60)

                                                                          ACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAG                  >  SRR4302540.5221695/1‑94 (MQ=60)

                                                                           CTTTGATTGCGGTTTTGTCGACCATCGCATCTAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGC                 <  SRR4302540.9551659/94‑1 (MQ=60)

                                                                           CTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGC                 >  SRR4302540.8647950/1‑94 (MQ=60)

                                                                           CTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGC                 >  SRR4302540.8647949/1‑94 (MQ=60)

                                                                           CTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGC                 <  SRR4302540.377036/94‑1 (MQ=60)

                                                                            TTTGATTGCGGTTTTGTCGACCATCGCATCAACCCCTTCGCGCCATTGTGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCG                <  SRR4302540.8718258/94‑1 (MQ=60)

                                                                            TTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCG                <  SRR4302540.9733903/94‑1 (MQ=60)

                                                                            TTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCG                <  SRR4302540.8718257/94‑1 (MQ=60)

                                                                            TTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCG                <  SRR4302540.3089824/94‑1 (MQ=60)

                                                                             TTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGC               <  SRR4302540.7581110/94‑1 (MQ=60)

                                                                              TGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCC              >  SRR4302540.9496784/1‑94 (MQ=60)

                                                                              TGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCC              <  SRR4302540.8660947/94‑1 (MQ=60)

                                                                              TGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCC              <  SRR4302540.2018062/94‑1 (MQ=60)

                                                                              TGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCC              <  SRR4302540.2034867/94‑1 (MQ=60)

                                                                               GATTGCGGTTTTGTCGACCATCGCATCAACCCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCG             <  SRR4302540.6985813/94‑1 (MQ=60)

                                                                               GATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCG             <  SRR4302540.5022730/94‑1 (MQ=60)

                                                                               GATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCG             <  SRR4302540.5450704/94‑1 (MQ=60)

                                                                                 TTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCT           <  SRR4302540.1744413/94‑1 (MQ=60)

                                                                                  TGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTG          <  SRR4302540.5838966/94‑1 (MQ=60)

                                                                                     GGTTTTGTCGACCATCGCATCAACCCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCT       <  SRR4302540.7965122/94‑1 (MQ=60)

                                                                                     GGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCT       >  SRR4302540.9497229/1‑94 (MQ=60)

                                                                                        TTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAAT    >  SRR4302540.969879/1‑94 (MQ=60)

                                                                                        TTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAAT    <  SRR4302540.6654390/94‑1 (MQ=60)

                                                                                         TTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAATA   <  SRR4302540.5724539/94‑1 (MQ=60)

                                                                                          TGTCGACCATCGCATCAACCCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAATAG  <  SRR4302540.1600138/94‑1 (MQ=60)

GGTTTTGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAATAG  >  NC_000913/1858234‑1858417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`