Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,919,873 G→A D24N (GAT→AAT)  b1836 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,919,8730GA99.3% 964.4 / ‑4.5 271D24N (GAT→AAT) b1836hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (133/136);  total (135/136)
Fisher's exact test for biased strand distribution p-value = 2.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 279 total aligned reads displayed.

AGCTAAATGGAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTGATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTATCCCTGCCCTG  >  NC_000913/1919783‑1919962
                                                                                          |                                                                                         
 gCTAAATGGAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATc                                                                                        >  1:8821780/1‑93 (MQ=255)
 gCTAAATGGAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATc                                                                                        >  1:194888/1‑93 (MQ=255)
 gCTAAATGGAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATc                                                                                        <  1:7062030/93‑1 (MQ=255)
  cTAAATGGAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATca                                                                                       >  1:1495137/1‑92 (MQ=255)
  cTAAATGGAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCg                                                                                       <  1:563645/93‑1 (MQ=255)
   tAAATGGAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGa                                                                                      >  1:7409019/1‑93 (MQ=255)
   tAAATGGAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGa                                                                                      >  2:6359080/1‑93 (MQ=255)
   tAAATGGAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGa                                                                                      >  2:7268492/1‑93 (MQ=255)
    aaaTGGAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGaa                                                                                     <  1:1021609/93‑1 (MQ=255)
    aaaTGGAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGaa                                                                                     >  2:3658767/1‑93 (MQ=255)
    aaaTGGAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGaa                                                                                     >  2:2367265/1‑93 (MQ=255)
    aaaTGGAGAGCACGAAGAAGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGaa                                                                                     <  2:8575072/93‑1 (MQ=255)
     aaTGGAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTGATCGAAc                                                                                    >  1:8608308/1‑93 (MQ=255)
      aTGGAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACg                                                                                   >  2:2981486/1‑93 (MQ=255)
       tGGAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGGACTACACGGTGAATCGCCAGGTAATCGTACGt                                                                                  >  1:4332468/1‑93 (MQ=255)
        ggAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGtt                                                                                 <  1:6104180/93‑1 (MQ=255)
         gagaGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTa                                                                                <  1:9828687/93‑1 (MQ=255)
          agagCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTaa                                                                               >  1:178082/1‑93 (MQ=255)
          agagCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTaa                                                                               <  2:7433084/93‑1 (MQ=255)
             gCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCa                                                                            <  1:437196/93‑1 (MQ=255)
              cACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCAt                                                                           <  2:9877674/93‑1 (MQ=255)
               aCGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCAtt                                                                          <  1:7823366/93‑1 (MQ=255)
               aCGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCAtt                                                                          >  1:4277828/1‑93 (MQ=255)
               aCGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCAtt                                                                          >  1:1300718/1‑93 (MQ=255)
               aCGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCAtt                                                                          >  2:6756814/1‑93 (MQ=255)
                  aaGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCt                                                                       >  2:873235/1‑93 (MQ=255)
                       gAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAAACATTCCTTGTaa                                                                  >  1:5707549/1‑93 (MQ=255)
                        aaaaCCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTaaa                                                                 <  1:651398/93‑1 (MQ=255)
                        aaaaCCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTaaa                                                                 >  1:9653230/1‑93 (MQ=255)
                         aaaCCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAAt                                                                >  2:6993311/1‑93 (MQ=255)
                           aCCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCt                                                              <  2:8941418/93‑1 (MQ=255)
                             cAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGa                                                            <  2:5508033/93‑1 (MQ=255)
                             cAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGa                                                            <  2:4097852/93‑1 (MQ=255)
                              aGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGAc                                                           <  1:9560575/93‑1 (MQ=255)
                               gtgtGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGAcc                                                          >  2:1891501/1‑93 (MQ=255)
                                tgtgCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGAccc                                                         <  1:630147/93‑1 (MQ=255)
                                 gtgCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGAcccc                                                        >  1:3920429/1‑93 (MQ=255)
                                  tgCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCg                                                       >  2:8646327/1‑93 (MQ=255)
                                  tgCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCg                                                       >  2:1638706/1‑93 (MQ=255)
                                  tgCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCg                                                       <  2:8165579/93‑1 (MQ=255)
                                  tgCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCg                                                       <  1:2154910/93‑1 (MQ=255)
                                    cgcATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGAt                                                     >  2:8903496/1‑93 (MQ=255)
                                     gcATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGAtt                                                    >  2:918604/1‑93 (MQ=255)
                                     gcATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGAtt                                                    >  1:7107353/1‑93 (MQ=255)
                                     gcATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGAtt                                                    >  2:9661459/1‑93 (MQ=255)
                                      cATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGAttt                                                   >  1:8050943/1‑93 (MQ=255)
                                      cATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATATGACCCCGAttt                                                   >  1:4129458/1‑93 (MQ=255)
                                       aTAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTTAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTta                                                  >  1:266842/1‑93 (MQ=255)
                                       aTAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGAtttt                                                  >  2:7161758/1‑92 (MQ=255)
                                        tAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTtat                                                 >  1:4881066/1‑93 (MQ=255)
                                        tAGGCGCTTTTGAAATCGACGAAGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTtat                                                 >  2:6406405/1‑93 (MQ=255)
                                         aGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTtatg                                                >  2:2503377/1‑93 (MQ=255)
                                          ggCGCTTTTGAAATCGACGACGGCGAATTACACGGAGAATCGCCAGGCAATCTGACGTTAACCATTCCTTGTAGATCTGACCCCCACTtatgt                                               >  2:2289368/1‑93 (MQ=255)
                                           gcgcTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTtatgta                                              <  1:2312906/93‑1 (MQ=255)
                                            cgcTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTtatgtat                                             >  1:7943106/1‑93 (MQ=255)
                                             gcTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTtatgtatg                                            <  1:7342132/93‑1 (MQ=255)
                                               ttttGAAATCGACGACGGCGAATTACGCGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCa                                          <  1:6823803/93‑1 (MQ=255)
                                                tttGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCaa                                         <  2:7944110/93‑1 (MQ=255)
                                                tttGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCaa                                         <  2:4657436/93‑1 (MQ=255)
                                                tttGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCaa                                         >  1:6424004/1‑93 (MQ=255)
                                                 ttGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAAc                                        <  1:3221505/93‑1 (MQ=255)
                                                 ttGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAAc                                        >  1:8504963/1‑93 (MQ=255)
                                                  tGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACt                                       <  2:2672419/93‑1 (MQ=255)
                                                  tGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACt                                       >  2:4871989/1‑93 (MQ=255)
                                                    aaaTCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTgg                                     <  2:4980734/93‑1 (MQ=255)
                                                       tCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATg                                  <  2:6038261/93‑1 (MQ=255)
                                                          acgacgGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCt                               <  2:9371620/93‑1 (MQ=255)
                                                           cgacgGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTg                              <  2:3584209/93‑1 (MQ=255)
                                                            gacgGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTgg                             <  1:3993167/93‑1 (MQ=255)
                                                            gacgGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTgg                             <  1:5290259/93‑1 (MQ=255)
                                                             acgGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTggg                            <  1:3700980/93‑1 (MQ=255)
                                                              cgGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGa                           <  2:7679157/93‑1 (MQ=255)
                                                              cgGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGa                           <  2:7805161/93‑1 (MQ=255)
                                                               ggCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGAt                          <  1:2595196/93‑1 (MQ=255)
                                                                gCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATg                         >  2:3353588/1‑93 (MQ=255)
                                                                 cGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGc                        >  2:5238048/1‑93 (MQ=255)
                                                                 cGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGc                        <  2:2910375/93‑1 (MQ=255)
                                                                  gAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCt                       <  2:4463221/93‑1 (MQ=255)
                                                                  gAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCt                       <  2:7226751/93‑1 (MQ=255)
                                                                   aaTTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTg                      >  1:6057235/1‑93 (MQ=255)
                                                                   aaTTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTg                      >  1:7233114/1‑93 (MQ=255)
                                                                      tACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGaaa                   >  1:4072451/1‑93 (MQ=255)
                                                                      tACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGaaa                   <  2:1044430/93‑1 (MQ=255)
                                                                         acGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCa                >  2:8498681/1‑93 (MQ=255)
                                                                         acGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCa                <  1:9410048/93‑1 (MQ=255)
                                                                          cGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAg               <  1:1705423/93‑1 (MQ=255)
                                                                            gTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTa             <  2:9812301/93‑1 (MQ=255)
                                                                            gTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTa             <  1:1142943/93‑1 (MQ=255)
                                                                            gTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTa             >  2:7364290/1‑93 (MQ=255)
                                                                            gTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTa             <  2:3936236/93‑1 (MQ=255)
                                                                            gTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTa             <  1:3389719/93‑1 (MQ=255)
                                                                              gAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTATc           >  2:505432/1‑93 (MQ=255)
                                                                              gAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTATc           >  2:1503147/1‑93 (MQ=255)
                                                                                aTCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTATccc         >  2:5807674/1‑93 (MQ=255)
                                                                                 tCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTATccct        >  1:6684293/1‑93 (MQ=255)
                                                                                  cGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTATccctg       <  1:4687466/93‑1 (MQ=255)
                                                                                   gCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTATccctgc      <  2:4233064/93‑1 (MQ=255)
                                                                                    ccAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTATccctgcc     >  2:4798357/1‑93 (MQ=255)
                                                                                    ccAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTATccctgcc     >  2:1384478/1‑93 (MQ=255)
                                                                                      aGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTATccctgccct   >  1:9765475/1‑93 (MQ=255)
                                                                                          |                                                                                         
AGCTAAATGGAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTGATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTATCCCTGCCCTG  >  NC_000913/1919783‑1919962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCAGCTAAATGGAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTGATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTATCCCTGCCCTGCTAA  >  NC_000913/1919780‑1919966
                                                                                             |                                                                                             
GGCAGCTAAATGGAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTA                                                                                               <  SRR4302540.1983323/94‑1 (MQ=60)
GGCAGCTAAATGGAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTA                                                                                               <  SRR4302540.5606830/94‑1 (MQ=60)
   AGCTAAATGGAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATC                                                                                            >  SRR4302540.9942732/1‑94 (MQ=60)
   AGCTAAATGGAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATC                                                                                            >  SRR4302540.196823/1‑94 (MQ=60)
   AGCTAAATGGAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATC                                                                                            >  SRR4302540.8923782/1‑94 (MQ=60)
    GCTAAATGGAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCG                                                                                           <  SRR4302540.7140580/94‑1 (MQ=60)
     CTAAATGGAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGA                                                                                          >  SRR4302540.7491549/1‑94 (MQ=60)
     CTAAATGGAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGA                                                                                          >  SRR4302540.6897427/1‑94 (MQ=60)
     CTAAATGGAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGA                                                                                          <  SRR4302540.569774/94‑1 (MQ=60)
     CTAAATGGAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGA                                                                                          <  SRR4302540.569776/94‑1 (MQ=60)
      TAAATGGAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAA                                                                                         >  SRR4302540.8220312/1‑94 (MQ=60)
       AAATGGAGAGCACGAAGATGAAACCCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAAC                                                                                        <  SRR4302540.230485/94‑1 (MQ=60)
       AAATGGAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTGATCGAAC                                                                                        >  SRR4302540.8708338/1‑94 (MQ=60)
       AAATGGAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAAC                                                                                        >  SRR4302540.1907528/1‑94 (MQ=60)
       AAATGGAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAAC                                                                                        <  SRR4302540.1031975/94‑1 (MQ=60)
       AAATGGAAAGCACGAAGAGGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAAC                                                                                        <  SRR4302540.6522255/94‑1 (MQ=60)
        AATGGAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACG                                                                                       <  SRR4302540.117009/94‑1 (MQ=60)
         ATGGAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGGACTACACGGTGAATCGCCAGGTAATCGTACGT                                                                                      >  SRR4302540.4379630/1‑94 (MQ=60)
         ATGGAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGT                                                                                      >  SRR4302540.4379631/1‑94 (MQ=60)
          TGGAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTT                                                                                     <  SRR4302540.1686227/94‑1 (MQ=60)
          TGGAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTT                                                                                     <  SRR4302540.8086376/94‑1 (MQ=60)
           GGAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTA                                                                                    >  SRR4302540.4052022/1‑94 (MQ=60)
           GGAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTA                                                                                    <  SRR4302540.6172153/94‑1 (MQ=60)
            GAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAA                                                                                   >  SRR4302540.6721107/1‑94 (MQ=60)
            GAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAA                                                                                   <  SRR4302540.9943655/94‑1 (MQ=60)
            GAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAA                                                                                   >  SRR4302540.179849/1‑94 (MQ=60)
             AGAGCCTGAAGATGAAAACCAGTGTGCCCACAGGCGATTTTGAAATCGACGGCGGCAAATTACACGGTAAATCGCCAGGTAATCGAACGTTAAC                                                                                  <  SRR4302540.6872930/94‑1 (MQ=60)
              GAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACC                                                                                 >  SRR4302540.7262214/1‑94 (MQ=60)
                GCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCGT                                                                               >  SRR4302540.6238515/1‑94 (MQ=60)
                GCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCAT                                                                               <  SRR4302540.441836/94‑1 (MQ=60)
                GCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCAT                                                                               <  SRR4302540.12293/94‑1 (MQ=60)
                 CACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATT                                                                              >  SRR4302540.4324354/1‑94 (MQ=60)
                 CACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATT                                                                              >  SRR4302540.1327884/1‑94 (MQ=60)
                 CACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATT                                                                              >  SRR4302540.1314202/1‑94 (MQ=60)
                  ACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTC                                                                             <  SRR4302540.7914792/94‑1 (MQ=60)
                    GAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCT                                                                           >  SRR4302540.8893758/1‑94 (MQ=60)
                    GAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCT                                                                           >  SRR4302540.6635548/1‑94 (MQ=60)
                     AAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTT                                                                          <  SRR4302540.8613327/94‑1 (MQ=60)
                      AGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTG                                                                         >  SRR4302540.5840018/1‑94 (MQ=60)
                      AGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTG                                                                         >  SRR4302540.200441/1‑94 (MQ=60)
                       GATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGT                                                                        <  SRR4302540.9803054/94‑1 (MQ=60)
                        ATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTA                                                                       >  SRR4302540.3706285/1‑94 (MQ=60)
                         TGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAAACATTCCTTGTAA                                                                      >  SRR4302540.5770386/1‑94 (MQ=60)
                          GAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAA                                                                     >  SRR4302540.8566159/1‑94 (MQ=60)
                          GAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAA                                                                     <  SRR4302540.1761559/94‑1 (MQ=60)
                          GAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAA                                                                     >  SRR4302540.9766886/1‑94 (MQ=60)
                           AAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAAT                                                                    <  SRR4302540.658327/94‑1 (MQ=60)
                            AAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATC                                                                   >  SRR4302540.954848/1‑94 (MQ=60)
                            AAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATC                                                                   <  SRR4302540.9569765/94‑1 (MQ=60)
                             AACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGTTAATCGAACGTTAACCATTCCTTGTAAATCT                                                                  <  SRR4302540.2461032/94‑1 (MQ=60)
                             AACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCT                                                                  >  SRR4302540.427723/1‑94 (MQ=60)
                               CCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGA                                                                >  SRR4302540.9512310/1‑94 (MQ=60)
                               ACAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGA                                                                <  SRR4302540.627998/94‑1 (MQ=60)
                                CAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGAC                                                               <  SRR4302540.5161197/94‑1 (MQ=60)
                                CAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGAC                                                               <  SRR4302540.15008/94‑1 (MQ=60)
                                CAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGGAATCGAACGTTAACCATTCCTTGTAAATCTGAC                                                               >  SRR4302540.3107668/1‑94 (MQ=60)
                                 AGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACC                                                              <  SRR4302540.9673606/94‑1 (MQ=60)
                                 AGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACC                                                              >  SRR4302540.4710028/1‑94 (MQ=60)
                                  GTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCC                                                             >  SRR4302540.5699390/1‑94 (MQ=60)
                                   TGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCC                                                            <  SRR4302540.636849/94‑1 (MQ=60)
                                   TGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCC                                                            >  SRR4302540.3962861/1‑94 (MQ=60)
                                    GTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCG                                                           <  SRR4302540.8002887/94‑1 (MQ=60)
                                     TGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGA                                                          <  SRR4302540.2178100/94‑1 (MQ=60)
                                       CGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATT                                                        >  SRR4302540.7186439/1‑94 (MQ=60)
                                       CGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATT                                                        <  SRR4302540.505635/94‑1 (MQ=60)
                                        GCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTT                                                       >  SRR4302540.8144449/1‑94 (MQ=60)
                                        GCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTT                                                       >  SRR4302540.4498295/1‑94 (MQ=60)
                                        GCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTT                                                       >  SRR4302540.7047772/1‑94 (MQ=60)
                                        GCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATATGACCCCGATTT                                                       >  SRR4302540.4174098/1‑94 (MQ=60)
                                         CATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTTAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTA                                                      >  SRR4302540.269470/1‑94 (MQ=60)
                                          ATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCTAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTAT                                                     >  SRR4302540.158257/1‑94 (MQ=60)
                                          ATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTAT                                                     >  SRR4302540.10023146/1‑94 (MQ=60)
                                          ATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTAT                                                     >  SRR4302540.4933477/1‑94 (MQ=60)
                                              GCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTAT                                                 <  SRR4302540.2337801/94‑1 (MQ=60)
                                              GCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTAT                                                 >  SRR4302540.8035686/1‑94 (MQ=60)
                                              GCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTAT                                                 <  SRR4302540.7530042/94‑1 (MQ=60)
                                               CGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGCTTTATGTATG                                                >  SRR4302540.4161252/1‑94 (MQ=60)
                                                GCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGC                                               <  SRR4302540.7424054/94‑1 (MQ=60)
                                                GCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGC                                               <  SRR4302540.3100601/94‑1 (MQ=60)
                                                 CTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCA                                              <  SRR4302540.6788861/94‑1 (MQ=60)
                                                  TTTTGAAATCGACGACGGCGAATTACGCGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAA                                             <  SRR4302540.6899344/94‑1 (MQ=60)
                                                  TTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAA                                             >  SRR4302540.7384876/1‑94 (MQ=60)
                                                  TTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAA                                             <  SRR4302540.7230004/94‑1 (MQ=60)
                                                  TTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAA                                             >  SRR4302540.6495508/1‑94 (MQ=60)
                                                   TTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAAC                                            >  SRR4302540.8603796/1‑94 (MQ=60)
                                                    TTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACT                                           <  SRR4302540.6127341/94‑1 (MQ=60)
                                                    TTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACT                                           <  SRR4302540.3256516/94‑1 (MQ=60)
                                                    TTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACT                                           <  SRR4302540.6127340/94‑1 (MQ=60)
                                                     TGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTG                                          <  SRR4302540.2221859/94‑1 (MQ=60)
                                                      GAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGG                                         <  SRR4302540.2569044/94‑1 (MQ=60)
                                                       AAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGA                                        >  SRR4302540.4811560/1‑94 (MQ=60)
                                                       AAATCGACGACGGCGAATTACACGGTGAATCGCCAGGGAATCGAACGTTAACCATTCGTTGTAAATCTGACCCCGATTTATGTATGCAACTGGA                                        >  SRR4302540.4811559/1‑94 (MQ=60)
                                                        agtcgacgacggccaactacacgggcaagccgCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGAT                                       <  SRR4302540.4023086/62‑1 (MQ=60)
                                                        AATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGAT                                       <  SRR4302540.713928/94‑1 (MQ=60)
                                                        AATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGAT                                       <  SRR4302540.5457572/94‑1 (MQ=60)
                                                        AATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGAT                                       <  SRR4302540.6467502/94‑1 (MQ=60)
                                                        AATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGAT                                       <  SRR4302540.5933902/94‑1 (MQ=60)
                                                         ATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATG                                      <  SRR4302540.8612896/94‑1 (MQ=60)
                                                         ATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATG                                      >  SRR4302540.7448710/1‑94 (MQ=60)
                                                         ATCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATG                                      <  SRR4302540.1914727/94‑1 (MQ=60)
                                                          TCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGC                                     >  SRR4302540.7475151/1‑94 (MQ=60)
                                                          TCGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGC                                     >  SRR4302540.6985830/1‑94 (MQ=60)
                                                           CGACGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCC                                    >  SRR4302540.3418035/1‑94 (MQ=60)
                                                              CGACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGG                                 <  SRR4302540.3818334/94‑1 (MQ=60)
                                                               GACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGG                                <  SRR4302540.5348091/94‑1 (MQ=60)
                                                               GACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGG                                <  SRR4302540.4036142/94‑1 (MQ=60)
                                                                ACGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGA                               <  SRR4302540.3741380/94‑1 (MQ=60)
                                                                 CGGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGAT                              <  SRR4302540.6522390/94‑1 (MQ=60)
                                                                  GGCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATG                             <  SRR4302540.2623185/94‑1 (MQ=60)
                                                                   GCGAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGC                            >  SRR4302540.2007682/1‑94 (MQ=60)
                                                                     GAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTTACCCCGGTTTATGTTTGCAACTGGATGCCTGGGATGTTG                          >  SRR4302540.2385600/1‑94 (MQ=60)
                                                                     GAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTG                          >  SRR4302540.7313785/1‑94 (MQ=60)
                                                                     GAATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTG                          >  SRR4302540.6124556/1‑94 (MQ=60)
                                                                      AATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGA                         >  SRR4302540.971671/1‑94 (MQ=60)
                                                                      AATTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGA                         >  SRR4302540.4140294/1‑94 (MQ=60)
                                                                        TTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAA                       >  SRR4302540.4116349/1‑94 (MQ=60)
                                                                        TTACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAA                       <  SRR4302540.4102224/94‑1 (MQ=60)
                                                                          ACACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACC                     >  SRR4302540.3959963/1‑94 (MQ=60)
                                                                            ACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAG                   <  SRR4302540.9521943/94‑1 (MQ=60)
                                                                            ACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAG                   >  SRR4302540.9058081/1‑94 (MQ=60)
                                                                            ACGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAG                   >  SRR4302540.8083651/1‑94 (MQ=60)
                                                                             CGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGT                  <  SRR4302540.7515280/94‑1 (MQ=60)
                                                                             CGGTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGT                  <  SRR4302540.1723862/94‑1 (MQ=60)
                                                                               GTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTAT                <  SRR4302540.1154888/94‑1 (MQ=60)
                                                                               GTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTAT                <  SRR4302540.3426702/94‑1 (MQ=60)
                                                                               GTGAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTAT                <  SRR4302540.4365414/94‑1 (MQ=60)
                                                                                 GAATCGCCAGGTAATCGATCGTTAACCATTCCTCGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTATCC              <  SRR4302540.10107898/94‑1 (MQ=60)
                                                                                 GAATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTATCA              <  SRR4302540.2692747/94‑1 (MQ=60)
                                                                                  AATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTATCCC             >  SRR4302540.7346197/1‑94 (MQ=60)
                                                                                  AATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTATCCC             >  SRR4302540.106700/1‑94 (MQ=60)
                                                                                  AATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTATCCC             <  SRR4302540.4370847/94‑1 (MQ=60)
                                                                                   ATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTATCCCT            >  SRR4302540.6758112/1‑94 (MQ=60)
                                                                                   ATCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTATCCCT            >  SRR4302540.3736504/1‑94 (MQ=60)
                                                                                    TCGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTATCCCTG           <  SRR4302540.341855/94‑1 (MQ=60)
                                                                                     CGCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTATCCCTGC          <  SRR4302540.4737596/94‑1 (MQ=60)
                                                                                      GCCAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTATCCCTGCC         >  SRR4302540.9750229/1‑94 (MQ=60)
                                                                                        CAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTATCCCTGCCCT       <  SRR4302540.4299227/94‑1 (MQ=60)
                                                                                        CAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTATCCCTGCCCT       >  SRR4302540.9880000/1‑94 (MQ=60)
                                                                                        CAGGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTATCCCTGCCCG       >  SRR4302540.9879999/1‑94 (MQ=60)
                                                                                          GGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTATCCCTGCCCTGC     >  SRR4302540.516309/1‑94 (MQ=60)
                                                                                          GGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTATCCCTGCCCTGC     >  SRR4302540.6040857/1‑94 (MQ=60)
                                                                                          GGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTATCCCTGCCCTGC     >  SRR4302540.4590373/1‑94 (MQ=60)
                                                                                          GGTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTATCCCTGCCCTGC     >  SRR4302540.7308804/1‑94 (MQ=60)
                                                                                           GTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTATCCCTGCCCTGCT    <  SRR4302540.9538689/94‑1 (MQ=60)
                                                                                           GTAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTATCCCTGCCCTGCT    >  SRR4302540.3156291/1‑94 (MQ=60)
                                                                                            TAATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGCAACCAGTATCCCTGCCCTGCTA   >  SRR4302540.6981379/1‑94 (MQ=60)
                                                                                             AATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTATCCCTGCCCTGCTAA  <  SRR4302540.2693881/94‑1 (MQ=60)
                                                                                             AATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTATCCCTGCCCTGCTAA  >  SRR4302540.3120124/1‑94 (MQ=60)
                                                                                             |                                                                                             
GGCAGCTAAATGGAGAGCACGAAGATGAAAACCAGTGTGCGCATAGGCGCTTTTGAAATCGACGACGGCGAATTACACGGTGAATCGCCAGGTGATCGAACGTTAACCATTCCTTGTAAATCTGACCCCGATTTATGTATGCAACTGGATGCCTGGGATGCTGAAACCAGTATCCCTGCCCTGCTAA  >  NC_000913/1919780‑1919966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑