Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,923,720	T→C	V86A (GTT→GCT)	b1843 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,923,720	0	T	C	99.6%	756.4 / ‑4.9	234	V86A (GTT→GCT)	b1843	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (119/114); total (120/114)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.14e-01

Only 100 of 237 total aligned reads displayed.

CCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGTGCAT  >  NC_000913/1923631‑1923809

ccTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGa                                                                                        <  2:4261428/93‑1 (MQ=255)

 cTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGaa                                                                                       <  2:619649/93‑1 (MQ=255)

  ttCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAat                                                                                      >  1:9146353/1‑93 (MQ=255)

  ttCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAat                                                                                      >  1:819097/1‑93 (MQ=255)

   tCCTGCCCCACCCGATCTCTCCCTTTTAGACACGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCTTGCCTTCCCGCTGCata                                                                                     >  2:7261024/1‑93 (MQ=255)

   tCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAata                                                                                     >  2:560090/1‑93 (MQ=255)

    ccTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAatat                                                                                    >  2:6011656/1‑93 (MQ=255)

     cTGCCCCACCCGATCTCTCACTGTTAGCCCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAAtata                                                                                   <  1:4815245/93‑1 (MQ=255)

     cTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAAtata                                                                                   <  2:3097919/93‑1 (MQ=255)

     cTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAAtata                                                                                   <  2:659032/93‑1 (MQ=255)

      tGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATaa                                                                                  <  2:8965311/93‑1 (MQ=255)

      tGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATaa                                                                                  <  2:2450501/93‑1 (MQ=255)

      tGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATaa                                                                                  <  2:6430738/93‑1 (MQ=255)

      cgCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATaa                                                                                  <  2:1893848/92‑1 (MQ=255)

       ggcccgcccgCTCTCTCTCTGGTAGACCCTCTTTGTTATGCTGCAACGACCTGGCGAATGACCATCATTGCCGGCCTCCCCGCTGAATAGAAc                                                                                 <  2:2675819/91‑1 (MQ=255)

         cccACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGa                                                                               <  2:3881837/93‑1 (MQ=255)

          ccACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGAt                                                                              <  1:4161713/93‑1 (MQ=255)

          ccACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGAt                                                                              <  2:5676344/93‑1 (MQ=255)

           cACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATc                                                                             <  2:8639242/93‑1 (MQ=255)

           cACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTCCCGGCCTCCCCGCTGAATATAACGATc                                                                             <  2:8510114/93‑1 (MQ=255)

             cccGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGc                                                                           <  1:4555022/93‑1 (MQ=255)

             cccGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGc                                                                           >  1:6365631/1‑93 (MQ=255)

              ccGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCt                                                                          <  2:6688192/93‑1 (MQ=255)

              ccGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCt                                                                          >  1:5805573/1‑93 (MQ=255)

                gATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCttt                                                                        >  1:8032353/1‑93 (MQ=255)

                 aTCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCtttt                                                                       >  2:5093999/1‑92 (MQ=255)

                 aTCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTa                                                                       >  1:9750548/1‑93 (MQ=255)

                 aTCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTa                                                                       >  2:2175223/1‑93 (MQ=255)

                 aTCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTa                                                                       >  1:10026473/1‑93 (MQ=255)

                 aTCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTa                                                                       <  1:7645136/93‑1 (MQ=255)

                   ctctcACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTAtt                                                                     <  2:8308964/93‑1 (MQ=255)

                   ctctcACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTAtt                                                                     >  2:6769409/1‑93 (MQ=255)

                    tctcACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTc                                                                    >  2:1102987/1‑93 (MQ=255)

                    tctcACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTc                                                                    <  1:5923213/93‑1 (MQ=255)

                      tcACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGt                                                                  <  1:2442596/93‑1 (MQ=255)

                      tcACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGt                                                                  >  1:2127398/1‑93 (MQ=255)

                      tcACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGt                                                                  >  1:3724133/1‑93 (MQ=255)

                       cACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTg                                                                 >  1:7242500/1‑93 (MQ=255)

                        aCTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATCTAACGATCGCTTTATTCGTgg                                                                >  2:5306524/1‑93 (MQ=255)

                         cTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGa                                                               >  2:4690035/1‑93 (MQ=255)

                         cTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGa                                                               >  1:9854541/1‑93 (MQ=255)

                          tGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGaa                                                              >  2:2296484/1‑93 (MQ=255)

                           gTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGTAAt                                                             >  1:7440574/1‑93 (MQ=255)

                            ttAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCGTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAAtt                                                            <  2:4915731/93‑1 (MQ=255)

                             tAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTg                                                           <  2:1288239/93‑1 (MQ=255)

                                aCCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCgg                                                        <  2:2882419/93‑1 (MQ=255)

                                 cccGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGgt                                                       >  2:5938356/1‑93 (MQ=255)

                                  ccGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGgtg                                                      <  1:5538834/93‑1 (MQ=255)

                                  ccGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGgtg                                                      >  1:3417033/1‑93 (MQ=255)

                                     cTTTGTTATGCCGCAACGACCTGGCGAATGCCCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTc                                                   <  1:3040432/93‑1 (MQ=255)

                                      tttGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCg                                                  >  1:1747067/1‑93 (MQ=255)

                                        tGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCa                                                >  2:9513722/1‑93 (MQ=255)

                                         gTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCAc                                               >  1:264894/1‑93 (MQ=255)

                                          ttATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCAcc                                              >  1:6993454/1‑93 (MQ=255)

                                            aTGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGt                                            >  1:7104877/1‑93 (MQ=255)

                                            aTGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGt                                            <  1:8284629/93‑1 (MQ=255)

                                              gCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTgg                                          <  1:4381405/93‑1 (MQ=255)

                                               ccGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGc                                         <  2:7141436/93‑1 (MQ=255)

                                                cGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCg                                        >  1:9742003/1‑93 (MQ=255)

                                                 gCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGa                                       <  1:6588904/93‑1 (MQ=255)

                                                     cGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGaaaaa                                   >  1:8516710/1‑93 (MQ=255)

                                                     cGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGaaaaa                                   <  1:7562873/93‑1 (MQ=255)

                                                     cGACCTGGCGAATGACCATCATTCCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGaaaaa                                   <  2:5532304/93‑1 (MQ=255)

                                                       aCCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACg                                 >  1:9581313/1‑93 (MQ=255)

                                                         cTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATTACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGcc                               <  2:2006607/93‑1 (MQ=255)

                                                         cTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGcc                               >  2:9701469/1‑93 (MQ=255)

                                                         cTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGcc                               >  1:288102/1‑93 (MQ=255)

                                                         cTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGcc                               >  1:3904325/1‑93 (MQ=255)

                                                          tGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCt                              <  2:1706335/93‑1 (MQ=255)

                                                          tGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCt                              >  2:8254368/1‑93 (MQ=255)

                                                          tGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCt                              >  1:4436382/1‑93 (MQ=255)

                                                           ggCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTg                             <  1:2582176/93‑1 (MQ=255)

                                                           ggCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTg                             <  2:6946658/93‑1 (MQ=255)

                                                              gAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGa                          >  2:9177828/1‑93 (MQ=255)

                                                              gAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGa                          <  2:3604805/93‑1 (MQ=255)

                                                               aaTGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGAt                         <  1:1643061/93‑1 (MQ=255)

                                                                aTGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATc                        <  2:3999042/93‑1 (MQ=255)

                                                                aTGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATc                        <  2:2613241/93‑1 (MQ=255)

                                                                   gccAGCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTAc                     <  2:1576390/92‑1 (MQ=255)

                                                                    ccATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTAcc                    <  1:8328540/93‑1 (MQ=255)

                                                                     catcatTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCa                   >  1:3962883/1‑93 (MQ=255)

                                                                     catcatTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCa                   >  1:1058046/1‑93 (MQ=255)

                                                                       tcatTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATc                 <  1:5140122/93‑1 (MQ=255)

                                                                       tcatTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATc                 <  1:2977129/93‑1 (MQ=255)

                                                                       tcatTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATc                 <  2:5168995/93‑1 (MQ=255)

                                                                          ttGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCaga              <  2:420636/93‑1 (MQ=255)

                                                                            gCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGt            <  1:5366552/93‑1 (MQ=255)

                                                                               ggCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCAt         >  2:7974142/1‑93 (MQ=255)

                                                                               ggCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCAt         >  1:2241841/1‑93 (MQ=255)

                                                                               ggCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGGGTTCGCACCGCGGCGAAAAACGCCTGGGATCTACCATCAGAGTCAt         >  2:3939665/1‑93 (MQ=255)

                                                                                gCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATg        >  1:2023980/1‑93 (MQ=255)

                                                                                 ccTTCCCGTTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATgg       >  1:7975986/1‑93 (MQ=255)

                                                                                 ccTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATgg       <  2:7877933/93‑1 (MQ=255)

                                                                                 ccTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATgg       <  1:6202246/93‑1 (MQ=255)

                                                                                 ccTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATgg       <  1:1365862/93‑1 (MQ=255)

                                                                                  cTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGt      <  2:7547054/93‑1 (MQ=255)

                                                                                   ttCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGTg     >  2:229953/1‑93 (MQ=255)

                                                                                   ttCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGTg     >  1:3537629/1‑93 (MQ=255)

                                                                                    tCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGTGc    >  1:4639698/1‑93 (MQ=255)

                                                                                    tCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGTGc    >  1:8770166/1‑93 (MQ=255)

CCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGTGCAT  >  NC_000913/1923631‑1923809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGTGCATGT  >  NC_000913/1923627‑1923811

GTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGC                                                                                             <  SRR4302540.2738613/94‑1 (MQ=60)

GTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGC                                                                                             >  SRR4302540.8140103/1‑94 (MQ=60)

 TGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCT                                                                                            <  SRR4302540.6398324/94‑1 (MQ=60)

     CTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCCACGACCTGGCGAGTGAACATCATTGCCGGCCTTCCCGCTGAAT                                                                                        >  SRR4302540.1708328/1‑94 (MQ=60)

     CTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAAT                                                                                        >  SRR4302540.2563045/1‑94 (MQ=60)

     CTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAAT                                                                                        >  SRR4302540.9256208/1‑94 (MQ=60)

     CTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAAT                                                                                        >  SRR4302540.827804/1‑94 (MQ=60)

       TCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCCTCATTGCCGGCCTTCCCGCTGAATAT                                                                                      >  SRR4302540.4533116/1‑94 (MQ=60)

        CCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATA                                                                                     >  SRR4302540.7093267/1‑94 (MQ=60)

         CTGCCCCACCCGATCTCTCACTGTTAGCCCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAA                                                                                    <  SRR4302540.4866785/94‑1 (MQ=60)

         CTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAA                                                                                    <  SRR4302540.4866786/94‑1 (MQ=60)

          TGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAAC                                                                                   <  SRR4302540.8454234/94‑1 (MQ=60)

            CCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGA                                                                                 >  SRR4302540.7854772/1‑94 (MQ=60)

             CCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGAT                                                                                >  SRR4302540.7000903/1‑94 (MQ=60)

              CCCCCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATC                                                                               <  SRR4302540.4207085/94‑1 (MQ=60)

              CCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATC                                                                               <  SRR4302540.4654997/94‑1 (MQ=60)

              CCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATC                                                                               <  SRR4302540.4206821/94‑1 (MQ=60)

              CCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATC                                                                               >  SRR4302540.1731790/1‑94 (MQ=60)

               CACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCG                                                                              <  SRR4302540.8437770/94‑1 (MQ=60)

                ACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGC                                                                             >  SRR4302540.6436509/1‑94 (MQ=60)

                 CCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGTCCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCT                                                                            <  SRR4302540.4604270/94‑1 (MQ=60)

                 CCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCT                                                                            >  SRR4302540.5590897/1‑94 (MQ=60)

                 CCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCT                                                                            >  SRR4302540.5869726/1‑94 (MQ=60)

                 CCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCT                                                                            <  SRR4302540.4604272/94‑1 (MQ=60)

                  CCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTT                                                                           >  SRR4302540.7660214/1‑94 (MQ=60)

                   CGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTT                                                                          >  SRR4302540.8125737/1‑94 (MQ=60)

                    GATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTA                                                                         >  SRR4302540.9864961/1‑94 (MQ=60)

                    GATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTA                                                                         >  SRR4302540.9864964/1‑94 (MQ=60)

                    GATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTA                                                                         <  SRR4302540.5872750/94‑1 (MQ=60)

                    GATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTA                                                                         >  SRR4302540.2640402/1‑94 (MQ=60)

                    GATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTA                                                                         <  SRR4302540.10024115/94‑1 (MQ=60)

                    GATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTA                                                                         >  SRR4302540.10143136/1‑94 (MQ=60)

                    GATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCCCTTTA                                                                         >  SRR4302540.9864962/1‑94 (MQ=60)

                     ATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTAT                                                                        <  SRR4302540.6606953/94‑1 (MQ=60)

                     ATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTAT                                                                        >  SRR4302540.9057981/1‑94 (MQ=60)

                     ATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTAT                                                                        <  SRR4302540.8106943/94‑1 (MQ=60)

                     ATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTAT                                                                        <  SRR4302540.7682040/94‑1 (MQ=60)

                     ATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTAT                                                                        <  SRR4302540.7731186/94‑1 (MQ=60)

                        TCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCG                                                                     <  SRR4302540.5988659/94‑1 (MQ=60)

                         CTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGT                                                                    <  SRR4302540.3380461/94‑1 (MQ=60)

                         CTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGT                                                                    >  SRR4302540.2150225/1‑94 (MQ=60)

                         CTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGT                                                                    >  SRR4302540.3764764/1‑94 (MQ=60)

                         CTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGT                                                                    >  SRR4302540.3790899/1‑94 (MQ=60)

                         CTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGT                                                                    >  SRR4302540.6793627/1‑94 (MQ=60)

                         CTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGAACATGATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGT                                                                    >  SRR4302540.4898201/1‑94 (MQ=60)

                          TCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTG                                                                   >  SRR4302540.7323291/1‑94 (MQ=60)

                          TCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTG                                                                   <  SRR4302540.2468901/94‑1 (MQ=60)

                            ACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGA                                                                 >  SRR4302540.9969710/1‑94 (MQ=60)

                             CTGTTAGACCCGCTTTGTTATGCCGCAACGACCGGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAA                                                                <  SRR4302540.9823722/94‑1 (MQ=60)

                              TGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGTAAT                                                               >  SRR4302540.7523477/1‑94 (MQ=60)

                              TGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGTAAT                                                               >  SRR4302540.7523180/1‑94 (MQ=60)

                              TGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAAT                                                               <  SRR4302540.1616197/94‑1 (MQ=60)

                               GTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATT                                                              <  SRR4302540.321373/94‑1 (MQ=60)

                                TTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTG                                                             <  SRR4302540.5852124/94‑1 (MQ=60)

                                 TAGACCCGCTTTTGTAGGCCGCAACGGCCTGTCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACTATCGCTTTATTCGTGGAATTTC                                                            <  SRR4302540.192974/94‑1 (MQ=60)

                                    ACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGT                                                         >  SRR4302540.1059521/1‑94 (MQ=60)

                                     CCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTG                                                        >  SRR4302540.3454215/1‑94 (MQ=60)

                                      CCGCTTTGTTATGCCGCAACGCCCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGT                                                       <  SRR4302540.2094567/94‑1 (MQ=60)

                                      CCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGT                                                       <  SRR4302540.5599840/94‑1 (MQ=60)

                                       CGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTT                                                      <  SRR4302540.2184756/94‑1 (MQ=60)

                                       CGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTT                                                      <  SRR4302540.7517025/94‑1 (MQ=60)

                                       CGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTT                                                      >  SRR4302540.5264385/1‑94 (MQ=60)

                                        GCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTC                                                     >  SRR4302540.1086330/1‑94 (MQ=60)

                                         CTTTGTTATGCCGCAACGACCTGGCGAATGCCCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCG                                                    <  SRR4302540.3073801/94‑1 (MQ=60)

                                         CTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCG                                                    >  SRR4302540.1765984/1‑94 (MQ=60)

                                           TTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCA                                                  >  SRR4302540.1516352/1‑94 (MQ=60)

                                            TGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCAC                                                 >  SRR4302540.267490/1‑94 (MQ=60)

                                             TTTATGCCGCCACGGCCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACC                                                <  SRR4302540.3599663/94‑1 (MQ=60)

                                             GTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACC                                                >  SRR4302540.7071074/1‑94 (MQ=60)

                                              TTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCG                                               <  SRR4302540.8695431/94‑1 (MQ=60)

                                               TATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGT                                              >  SRR4302540.6233611/1‑94 (MQ=60)

                                               TATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGT                                              >  SRR4302540.7183927/1‑94 (MQ=60)

                                                ATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTG                                             <  SRR4302540.8380855/94‑1 (MQ=60)

                                                ATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTG                                             <  SRR4302540.8380857/94‑1 (MQ=60)

                                                  GCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGC                                           <  SRR4302540.4428980/94‑1 (MQ=60)

                                                  GCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGC                                           <  SRR4302540.4428978/94‑1 (MQ=60)

                                                   CCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCG                                          >  SRR4302540.9856350/1‑94 (MQ=60)

                                                     GCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAA                                        <  SRR4302540.6661883/94‑1 (MQ=60)

                                                       AACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTCGGGAAAA                                      >  SRR4302540.5641622/1‑94 (MQ=60)

                                                        ACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAA                                     >  SRR4302540.8615685/1‑94 (MQ=60)

                                                         CGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAAC                                    <  SRR4302540.7647698/94‑1 (MQ=60)

                                                          GACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACG                                   >  SRR4302540.9694454/1‑94 (MQ=60)

                                                          GACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACC                                   >  SRR4302540.2927845/1‑94 (MQ=60)

                                                           ACCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGC                                  >  SRR4302540.609773/1‑94 (MQ=60)

                                                            CCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCC                                 >  SRR4302540.290945/1‑94 (MQ=60)

                                                            CCTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCC                                 >  SRR4302540.3946667/1‑94 (MQ=60)

                                                             CTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCT                                <  SRR4302540.2844299/94‑1 (MQ=60)

                                                             CTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCT                                <  SRR4302540.2844300/94‑1 (MQ=60)

                                                             CTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCT                                >  SRR4302540.9927690/1‑94 (MQ=60)

                                                             CTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCT                                >  SRR4302540.2294116/1‑94 (MQ=60)

                                                             CTGGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCT                                >  SRR4302540.4484454/1‑94 (MQ=60)

                                                               GGCGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGG                              <  SRR4302540.2610012/94‑1 (MQ=60)

                                                                 CGAATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGA                            >  SRR4302540.7530827/1‑94 (MQ=60)

                                                                   AATGACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATC                          <  SRR4302540.1661013/94‑1 (MQ=60)

                                                                       ACCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACC                      >  SRR4302540.3042273/1‑94 (MQ=60)

                                                                        CCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCA                     <  SRR4302540.8425202/94‑1 (MQ=60)

                                                                        CCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCA                     >  SRR4302540.1068817/1‑94 (MQ=60)

                                                                        CCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCA                     >  SRR4302540.4005620/1‑94 (MQ=60)

                                                                        CCATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCA                     >  SRR4302540.9508287/1‑94 (MQ=60)

                                                                          ATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATC                   >  SRR4302540.4117056/1‑94 (MQ=60)

                                                                          ATCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGCATCTACCATC                   >  SRR4302540.6337389/1‑94 (MQ=60)

                                                                           TCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTTGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCA                  <  SRR4302540.1992215/94‑1 (MQ=60)

                                                                           TCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCA                  <  SRR4302540.5195801/94‑1 (MQ=60)

                                                                           TCATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCA                  <  SRR4302540.3010026/94‑1 (MQ=60)

                                                                            CATTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAG                 >  SRR4302540.4821576/1‑94 (MQ=60)

                                                                             CTTGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGA                <  SRR4302540.3548701/94‑1 (MQ=60)

                                                                               TGCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGT              >  SRR4302540.8273762/1‑94 (MQ=60)

                                                                                GCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTC             >  SRR4302540.3795405/1‑94 (MQ=60)

                                                                                GCCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTC             <  SRR4302540.5425517/94‑1 (MQ=60)

                                                                                 CCGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCA            <  SRR4302540.4026928/94‑1 (MQ=60)

                                                                                  CGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCAT           <  SRR4302540.2522566/94‑1 (MQ=60)

                                                                                  CGGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCAT           >  SRR4302540.2265933/1‑94 (MQ=60)

                                                                                   GGCCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATG          >  SRR4302540.2045851/1‑94 (MQ=60)

                                                                                    GCCTTCCCGTTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGG         >  SRR4302540.8068904/1‑94 (MQ=60)

                                                                                     CCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGT        >  SRR4302540.3959125/1‑94 (MQ=60)

                                                                                     CCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGT        <  SRR4302540.6271284/94‑1 (MQ=60)

                                                                                     CCTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGT        <  SRR4302540.1380310/94‑1 (MQ=60)

                                                                                      CTTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGTG       >  SRR4302540.3576254/1‑94 (MQ=60)

                                                                                       TTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGTGC      >  SRR4302540.8871804/1‑94 (MQ=60)

                                                                                       TTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGTGC      <  SRR4302540.8889452/94‑1 (MQ=60)

                                                                                       TTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGTGC      <  SRR4302540.8280935/94‑1 (MQ=60)

                                                                                       TTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGTGC      >  SRR4302540.4689374/1‑94 (MQ=60)

                                                                                       TTCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGCGTTCGCACCGTGGCGAAAAACGCCTGGCCTCTACCATCAGAGTCATGTTGC      >  SRR4302540.2079336/1‑94 (MQ=60)

                                                                                        TCCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGTGCA     >  SRR4302540.832659/1‑94 (MQ=60)

                                                                                         CCCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGTGCAT    >  SRR4302540.7212590/1‑94 (MQ=60)

                                                                                          CCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGTGCATG   >  SRR4302540.8258515/1‑94 (MQ=60)

                                                                                          CCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGTGCATG   >  SRR4302540.8258516/1‑94 (MQ=60)

                                                                                          CCGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGTGCATG   >  SRR4302540.7078457/1‑94 (MQ=60)

                                                                                           CGCTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGTGCATGT  >  SRR4302540.2837598/1‑94 (MQ=60)

GTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGAAAAACGCCTGGGATCTACCATCAGAGTCATGGTGCATGT  >  NC_000913/1923627‑1923811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`