Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,924,185 A→G G14G (GGA→GGG)  b1844 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,924,1850AG99.6% 713.5 / ‑3.9 223coding (42/663 nt)b1844hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (118/104);  minor base . (0/1);  total (118/105)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01

Only 100 of 244 total aligned reads displayed.

AAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGATCGTCCTATTAG  >  NC_000913/1924095‑1924274
                                                                                           |                                                                                         
 aGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCa‑ggggggg                                                                                        <  1:1821447/93‑1 (MQ=255)
   gCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCa‑gggggggga                                                                                      <  2:451332/93‑3 (MQ=255)
    cGATATCATTCCATGACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATc                                                                                     <  1:5311939/93‑1 (MQ=255)
      atatCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGt                                                                                   >  2:3969642/1‑93 (MQ=255)
       tatCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGTATCGtt                                                                                  <  1:9815356/93‑1 (MQ=255)
       tatCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGtt                                                                                  >  2:2642707/1‑93 (MQ=255)
       tatCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGtt                                                                                  <  2:3507299/93‑1 (MQ=255)
        atCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTg                                                                                 >  1:10028383/1‑93 (MQ=255)
         tCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTga                                                                                >  2:608165/1‑93 (MQ=255)
           aTTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTgaga                                                                              >  2:9443896/1‑93 (MQ=255)
            ttCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGAt                                                                             <  1:1294906/93‑1 (MQ=255)
            ttCCATTACGCTAGGCTTTTTCAGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGAt                                                                             >  1:232070/1‑93 (MQ=255)
             tCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGAtt                                                                            >  2:4667389/1‑93 (MQ=255)
             tCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGAtt                                                                            >  1:9401122/1‑93 (MQ=255)
              ccATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTg                                                                           >  1:7369428/1‑93 (MQ=255)
                aTTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGcc                                                                         >  2:287285/1‑93 (MQ=255)
                 ttACGCTAGGCTTTTTCGGTCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCct                                                                        >  1:9741783/1‑93 (MQ=255)
                  tACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCctc                                                                       <  2:4936014/93‑1 (MQ=255)
                  tACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCctc                                                                       >  1:759502/1‑93 (MQ=255)
                  tACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCctc                                                                       >  2:3028579/1‑93 (MQ=255)
                  tACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCctc                                                                       <  2:9237438/93‑1 (MQ=255)
                    cGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTg                                                                     <  1:4154247/93‑1 (MQ=255)
                     gCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGt                                                                    >  1:1089092/1‑93 (MQ=255)
                      cTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGtt                                                                   <  2:7686099/93‑1 (MQ=255)
                      cTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGtt                                                                   >  2:1147119/1‑93 (MQ=255)
                       tAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTg                                                                  >  1:596775/1‑93 (MQ=255)
                        aGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGa                                                                 >  2:7581949/1‑93 (MQ=255)
                        aGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGa                                                                 <  1:6388536/93‑1 (MQ=255)
                        aGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGa                                                                 <  2:5576255/93‑1 (MQ=255)
                         ggCTTTTTCGGCCTGGAGCATGACATGTTGAGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGAGCGCTGAGATTGATTCTGCTGAt                                                                >  2:2185655/1‑93 (MQ=255)
                          gCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATg                                                               >  1:7821916/1‑93 (MQ=255)
                            tttttCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTc                                                             >  2:8409352/1‑93 (MQ=255)
                             ttttCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCa                                                            >  1:9851323/1‑93 (MQ=255)
                             ttttCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCa                                                            >  2:9851212/1‑93 (MQ=255)
                             ttttCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCa                                                            >  1:2141434/1‑93 (MQ=255)
                              tttCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCAt                                                           >  1:3057836/1‑93 (MQ=255)
                              tttCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCAt                                                           <  2:2285196/93‑1 (MQ=255)
                               ttCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCAtt                                                          >  1:7479121/1‑93 (MQ=255)
                                   gCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACg                                                      <  1:3326666/93‑1 (MQ=255)
                                   gCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACg                                                      >  2:4079176/1‑93 (MQ=255)
                                    ccTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACgg                                                     >  1:5328010/1‑93 (MQ=255)
                                    ccTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACgg                                                     >  1:7884830/1‑93 (MQ=255)
                                    ccTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACgg                                                     >  1:5109807/1‑93 (MQ=255)
                                       ggAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGaaa                                                  >  2:2470800/1‑93 (MQ=255)
                                         aGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGaaaaa                                                <  1:8325203/93‑1 (MQ=255)
                                         aGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGaaaaa                                                <  1:9526005/93‑1 (MQ=255)
                                          gCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAAt                                               <  2:4418214/93‑1 (MQ=255)
                                          gCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAAt                                               >  1:5440352/1‑93 (MQ=255)
                                          gCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAAt                                               <  1:6703710/93‑1 (MQ=255)
                                           cATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATc                                              <  2:1765213/93‑1 (MQ=255)
                                             tGCCATGTTGCGCATTATCGATACAGACACCTGCGGTTTGCA‑GGGGGGGCTCGTTGCGATTGTGTCTGTTGATGTGATTGTCGGAAAAAtaga                                            >  1:5172794/1‑90 (MQ=255)
                                             tGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGt                                            <  1:6944864/93‑1 (MQ=255)
                                             tGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGt                                            >  1:5172792/1‑93 (MQ=255)
                                               ccATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTc                                           >  1:3895319/1‑93 (MQ=255)
                                               ccATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCa                                          <  2:1561144/93‑1 (MQ=255)
                                                cATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCaa                                         <  2:4329807/93‑1 (MQ=255)
                                                cATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCaa                                         <  2:9188525/93‑1 (MQ=255)
                                                cATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCaa                                         >  2:8709972/1‑93 (MQ=255)
                                                cATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCATCaa                                         >  2:4838599/1‑93 (MQ=255)
                                                 aTGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAAc                                        >  1:8545614/1‑93 (MQ=255)
                                                  tGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAAcc                                       >  2:6530311/1‑93 (MQ=255)
                                                   gTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAAccc                                      >  2:8317697/1‑93 (MQ=255)
                                                   gTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAAccc                                      <  2:5750268/93‑1 (MQ=255)
                                                   gTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAAccc                                      >  1:3108643/1‑93 (MQ=255)
                                                     tGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCa                                    <  1:2774392/93‑1 (MQ=255)
                                                        gcATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAATATCGTCAACCCCATGa                                 >  1:8968468/1‑93 (MQ=255)
                                                        gcATTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGa                                 <  2:5199484/93‑1 (MQ=255)
                                                        gcATTATCGATACAGAAACCTGCGGTTGGCAGGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATg                                  <  1:3659413/93‑1 (MQ=255)
                                                         ctttATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAg                                >  1:4509447/3‑93 (MQ=255)
                                                         cATTATCGAAACAAAAACCTGCGGTTTGCA‑GGGGAGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAg                                <  2:7243349/93‑1 (MQ=255)
                                                          aTTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGc                               >  1:4859240/1‑93 (MQ=255)
                                                          aTTATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGc                               >  1:8039689/1‑93 (MQ=255)
                                                           ttATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGcc                              <  2:7348039/93‑1 (MQ=255)
                                                           ttATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGcc                              <  1:1065851/93‑1 (MQ=255)
                                                            tATCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGccc                             >  1:7142510/1‑92 (MQ=255)
                                                             aTCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCAc                            >  1:2736472/1‑93 (MQ=255)
                                                              tCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCCTGAGCCAcc                           >  2:204213/1‑93 (MQ=255)
                                                              tCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCAcc                           >  1:1183643/1‑93 (MQ=255)
                                                              tCGATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCAcc                           <  2:6433367/93‑1 (MQ=255)
                                                                gATACAGAAGCCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTg                         <  1:7688195/93‑1 (MQ=255)
                                                                gATACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTg                         >  2:8080423/1‑93 (MQ=255)
                                                                  tACAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGt                       >  2:8138088/1‑93 (MQ=255)
                                                                    cAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTgc                     >  1:8845608/1‑93 (MQ=255)
                                                                     aGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTgcg                    >  1:3276386/1‑93 (MQ=255)
                                                                     aGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTgcg                    >  2:6713507/1‑93 (MQ=255)
                                                                      gAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTgcgc                   <  1:4543651/93‑1 (MQ=255)
                                                                      gAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTgcgc                   >  1:1629324/1‑93 (MQ=255)
                                                                       aaaCCTGCGGTTTGCA‑GGG‑GGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGccc                 <  1:8704201/93‑1 (MQ=255)
                                                                        aaCCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGccc                 >  2:3271125/1‑93 (MQ=255)
                                                                         aCCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGcccc                >  2:783593/1‑93 (MQ=255)
                                                                          ccTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCg               <  2:1861513/93‑1 (MQ=255)
                                                                               ggTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGATCGt          >  2:6400350/1‑93 (MQ=255)
                                                                                gTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGATCGTc         >  1:6465458/1‑93 (MQ=255)
                                                                                gTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGATCGTc         <  1:2093788/93‑1 (MQ=255)
                                                                                   tGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGATCGTCCTa      <  2:4288241/93‑1 (MQ=255)
                                                                                   tGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGATCGTCCTa      <  1:6497590/93‑1 (MQ=255)
                                                                                    gCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGATCGTCCTAt     <  1:8199540/93‑1 (MQ=255)
                                                                                     ga‑gggggggATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGATCGTCCTAtt    <  2:7193604/92‑1 (MQ=255)
                                                                                     cA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGATCGTCCTAtt    <  2:6920804/93‑1 (MQ=255)
                                                                                      a‑gggggggATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGATCGTCCTATTa   <  2:8468166/93‑1 (MQ=255)
                                                                                           |                                                                                         
AAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCA‑GGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGATCGTCCTATTAG  >  NC_000913/1924095‑1924274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGATCGTCCTATTAGTCCA  >  NC_000913/1924092‑1924278
                                                                                                 |                                                                                             
GGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGG                                                                                               <  SRR4302540.3719764/94‑1 (MQ=60)
GGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGG                                                                                               <  SRR4302540.7827633/94‑1 (MQ=60)
GGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGG                                                                                               >  SRR4302540.5288555/1‑94 (MQ=60)
GGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGG                                                                                               >  SRR4302540.1250746/1‑94 (MQ=60)
  CAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGG                                                                                             >  SRR4302540.1724786/1‑94 (MQ=60)
  CAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGG                                                                                             <  SRR4302540.5848681/94‑1 (MQ=60)
  CAAGGCAATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGG                                                                                             <  SRR4302540.5848683/94‑1 (MQ=60)
   AAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGG                                                                                            <  SRR4302540.9531756/94‑1 (MQ=60)
    AGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGA                                                                                           <  SRR4302540.1841349/94‑1 (MQ=60)
      GCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATC                                                                                         <  SRR4302540.4789166/94‑1 (MQ=60)
       CGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCG                                                                                        <  SRR4302540.8013244/94‑1 (MQ=60)
       CGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCG                                                                                        >  SRR4302540.5183596/1‑94 (MQ=60)
       CGATATCATTCCATGACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCG                                                                                        <  SRR4302540.5370095/94‑1 (MQ=60)
        GATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGT                                                                                       >  SRR4302540.8424382/1‑94 (MQ=60)
          TATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGTATCGTTG                                                                                     <  SRR4302540.9930212/94‑1 (MQ=60)
          TATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTG                                                                                     <  SRR4302540.6415179/94‑1 (MQ=60)
          TATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTG                                                                                     >  SRR4302540.2769544/1‑94 (MQ=60)
          TATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTG                                                                                     <  SRR4302540.7103394/94‑1 (MQ=60)
          TATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTG                                                                                     >  SRR4302540.10145056/1‑94 (MQ=60)
             CATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGA                                                                                  >  SRR4302540.8237560/1‑94 (MQ=60)
              ATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGAT                                                                                 <  SRR4302540.9155978/94‑1 (MQ=60)
              ATTCCATTACGCTAGGCTTTTTCAGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGAT                                                                                 >  SRR4302540.234312/1‑94 (MQ=60)
               TTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATT                                                                                <  SRR4302540.1308309/94‑1 (MQ=60)
               TTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATT                                                                                >  SRR4302540.9512962/1‑94 (MQ=60)
                TCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTG                                                                               >  SRR4302540.7451576/1‑94 (MQ=60)
                   ATTACGCTAGGCTTTTTCGGTCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCT                                                                            >  SRR4302540.9856130/1‑94 (MQ=60)
                   ATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCT                                                                            >  SRR4302540.537837/1‑94 (MQ=60)
                    TTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTC                                                                           >  SRR4302540.767585/1‑94 (MQ=60)
                     TACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCT                                                                          <  SRR4302540.6546398/94‑1 (MQ=60)
                       CGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGT                                                                        <  SRR4302540.4199247/94‑1 (MQ=60)
                       CGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGT                                                                        >  SRR4302540.1100270/1‑94 (MQ=60)
                       CGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGT                                                                        >  SRR4302540.1100271/1‑94 (MQ=60)
                       CGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGT                                                                        >  SRR4302540.8930148/1‑94 (MQ=60)
                        GCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTT                                                                       >  SRR4302540.6902162/1‑94 (MQ=60)
                        GCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTT                                                                       <  SRR4302540.9058957/94‑1 (MQ=60)
                         CTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTG                                                                      >  SRR4302540.603184/1‑94 (MQ=60)
                          TAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGA                                                                     >  SRR4302540.4678803/1‑94 (MQ=60)
                          TAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGA                                                                     >  SRR4302540.250561/1‑94 (MQ=60)
                           AGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGAT                                                                    <  SRR4302540.1237744/94‑1 (MQ=60)
                           AGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGAT                                                                    <  SRR4302540.6459654/94‑1 (MQ=60)
                           AGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGAT                                                                    <  SRR4302540.6459655/94‑1 (MQ=60)
                            GGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTtctg                                                                   >  SRR4302540.7913327/1‑90 (MQ=60)
                            GGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATG                                                                   >  SRR4302540.7913326/1‑94 (MQ=60)
                             GCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGT                                                                  <  SRR4302540.10150001/94‑1 (MQ=60)
                              CTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTC                                                                 >  SRR4302540.5518594/1‑94 (MQ=60)
                               TTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCA                                                                >  SRR4302540.9966466/1‑94 (MQ=60)
                               TTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCA                                                                >  SRR4302540.2164446/1‑94 (MQ=60)
                                TTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAACCCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCAT                                                               <  SRR4302540.3331353/94‑1 (MQ=60)
                                TTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCAT                                                               >  SRR4302540.3091386/1‑94 (MQ=60)
                                TTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCAT                                                               <  SRR4302540.7395416/94‑1 (MQ=60)
                                 TTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATT                                                              >  SRR4302540.7562591/1‑94 (MQ=60)
                                 TTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATT                                                              >  SRR4302540.7427050/1‑94 (MQ=60)
                                   TCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGA                                                            <  SRR4302540.6744638/94‑1 (MQ=60)
                                   TCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGA                                                            >  SRR4302540.2129030/1‑94 (MQ=60)
                                    CGGCCAGGAGCATGACAAGTTGCGCATTA‑‑‑ATAAATCAGAAAGCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGAC                                                           <  SRR4302540.1195203/94‑1 (MQ=53)
                                      GCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGG                                                         >  SRR4302540.5386374/1‑94 (MQ=60)
                                      GCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGG                                                         >  SRR4302540.5165111/1‑94 (MQ=60)
                                      GCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGG                                                         <  SRR4302540.3363174/94‑1 (MQ=60)
                                      GCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGG                                                         >  SRR4302540.7976888/1‑94 (MQ=60)
                                      GCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑AAGAAGCCTGCGCTTCGCA‑GGGGGGGATCGTTGAGATTGACTCTGTTGATGTCATTGACGG                                                         <  SRR4302540.220765/94‑1 (MQ=60)
                                       CCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGA                                                        >  SRR4302540.963548/1‑94 (MQ=60)
                                       CCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGA                                                        >  SRR4302540.9163906/1‑94 (MQ=60)
                                       CCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGA                                                        >  SRR4302540.3088122/1‑94 (MQ=60)
                                        CTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGGA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAA                                                       <  SRR4302540.8900909/94‑1 (MQ=60)
                                        CTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGAGCGTTGAGATTGCCTCTGTTGATGTCATTGACGGGA                                                       >  SRR4302540.2106456/1‑94 (MQ=60)
                                            AGCATTCCATGTTGCGCATTATCGATA‑‑‑CAGAAGCCTGCGGTTTGCA‑GGGGGCGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAAT                                                   <  SRR4302540.5167728/94‑1 (MQ=60)
                                            AGCATGTCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAAT                                                   <  SRR4302540.9638796/94‑1 (MQ=60)
                                            AGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAAT                                                   <  SRR4302540.8421825/94‑1 (MQ=60)
                                            AGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAAT                                                   >  SRR4302540.377366/1‑94 (MQ=60)
                                            AGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAAT                                                   <  SRR4302540.8421827/94‑1 (MQ=60)
                                            AGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAAT                                                   <  SRR4302540.9638795/94‑1 (MQ=60)
                                            AGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAAT                                                   >  SRR4302540.5500208/1‑94 (MQ=60)
                                            AGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAAT                                                   >  SRR4302540.5500207/1‑94 (MQ=60)
                                            AGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAAT                                                   >  SRR4302540.7485202/1‑94 (MQ=60)
                                             GCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATC                                                  <  SRR4302540.6777699/94‑1 (MQ=60)
                                             GCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATC                                                  <  SRR4302540.3033483/94‑1 (MQ=60)
                                             GCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATC                                                  <  SRR4302540.1984189/94‑1 (MQ=60)
                                             GCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATC                                                  <  SRR4302540.1465844/94‑1 (MQ=60)
                                              CATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCG                                                 >  SRR4302540.7661324/1‑94 (MQ=60)
                                              CATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCG                                                 <  SRR4302540.8107337/94‑1 (MQ=60)
                                               ATGCCATGTTGCGCATTATCGATA‑‑‑CAGACACCTGCGGTTTGCA‑GGGGGGGCTCGTTGCGATTGTGTCTGTTGATGTGATTGTCGGAAAAATaga                                                >  SRR4302540.5229094/1‑91 (MQ=59)
                                               ATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCAGGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCG                                                 <  SRR4302540.1189587/94‑1 (MQ=60)
                                               ATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGT                                                >  SRR4302540.5229093/1‑94 (MQ=60)
                                               ATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGT                                                >  SRR4302540.5229092/1‑94 (MQ=60)
                                                TGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTC                                               <  SRR4302540.7022163/94‑1 (MQ=60)
                                                 GCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCAGGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTC                                               >  SRR4302540.3937598/1‑94 (MQ=60)
                                                 GCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCA                                              <  SRR4302540.3310545/94‑1 (MQ=60)
                                                 GCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCA                                              >  SRR4302540.7756735/1‑94 (MQ=60)
                                                  CCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAA                                             >  SRR4302540.7593697/1‑94 (MQ=60)
                                                   CATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAAC                                            >  SRR4302540.8644898/1‑94 (MQ=60)
                                                     TGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCC                                          >  SRR4302540.3142663/1‑94 (MQ=60)
                                                      GTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCC                                         >  SRR4302540.3096697/1‑94 (MQ=60)
                                                      GTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCC                                         <  SRR4302540.4239037/94‑1 (MQ=60)
                                                       TTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCA                                        <  SRR4302540.8658707/94‑1 (MQ=60)
                                                        TGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCAT                                       <  SRR4302540.2804374/94‑1 (MQ=60)
                                                         GCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGCCGGAAAAATCGTCAACCCCATG                                      >  SRR4302540.4527009/1‑94 (MQ=60)
                                                          CGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAATATCGTCAACCCCATGA                                     >  SRR4302540.9074848/1‑94 (MQ=60)
                                                          CGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGA                                     >  SRR4302540.4091412/1‑94 (MQ=60)
                                                           GCTTTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAG                                    >  SRR4302540.4558310/1‑94 (MQ=60)
                                                           GCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCAGGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGA                                     <  SRR4302540.3283839/94‑1 (MQ=60)
                                                           GCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAG                                    >  SRR4302540.4558311/1‑94 (MQ=60)
                                                           GCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAG                                    >  SRR4302540.5831197/1‑94 (MQ=60)
                                                           GCATTATCGATA‑‑‑CAGAAACCTGCGGTTGGCAGGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGA                                     <  SRR4302540.3699477/94‑1 (MQ=60)
                                                            CATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGC                                   >  SRR4302540.4911340/1‑94 (MQ=60)
                                                            CATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGC                                   >  SRR4302540.8133116/1‑94 (MQ=60)
                                                             ATTATCGATA‑‑‑CAGAAACCTGCGGTTTGTA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCC                                  >  SRR4302540.161031/1‑94 (MQ=60)
                                                             ATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCC                                  >  SRR4302540.6776882/1‑94 (MQ=60)
                                                              TTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCC                                 >  SRR4302540.7222085/1‑94 (MQ=60)
                                                              TTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCC                                 >  SRR4302540.5308064/1‑94 (MQ=60)
                                                              TTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCA                                 <  SRR4302540.1076714/94‑1 (MQ=60)
                                                              TTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCA                                 <  SRR4302540.7903887/94‑1 (MQ=60)
                                                              TTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCA                                 <  SRR4302540.7903891/94‑1 (MQ=60)
                                                               TATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCAC                                >  SRR4302540.10000000/1‑94 (MQ=60)
                                                               TATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCAC                                >  SRR4302540.2766007/1‑94 (MQ=60)
                                                                ATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACC                               >  SRR4302540.1195864/1‑94 (MQ=60)
                                                                  CGATA‑‑‑CAGAAACCTGCGGTTTGCAGGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCT                              >  SRR4302540.2451318/1‑94 (MQ=60)
                                                                   GATA‑‑‑CAGAAGCCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGG                            <  SRR4302540.7775485/94‑1 (MQ=60)
                                                                      A‑‑‑CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGC                         >  SRR4302540.8947932/1‑94 (MQ=60)
                                                                          CAGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCG                        >  SRR4302540.3312167/1‑94 (MQ=60)
                                                                           AGAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGC                       >  SRR4302540.1647161/1‑94 (MQ=60)
                                                                            GAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCC                      >  SRR4302540.9912845/1‑94 (MQ=60)
                                                                            GAAACCTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCC                      <  SRR4302540.4592817/94‑1 (MQ=60)
                                                                             AAACCTGCGGTTTGCA‑GGG‑GGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCC                    <  SRR4302540.8805239/94‑1 (MQ=60)
                                                                                 CTGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGAT                 <  SRR4302540.9966355/94‑1 (MQ=60)
                                                                                  TGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGATC                <  SRR4302540.345983/94‑1 (MQ=60)
                                                                                  TGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGATC                <  SRR4302540.4968087/94‑1 (MQ=60)
                                                                                  CGCGGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGATC                <  SRR4302540.5269457/94‑1 (MQ=60)
                                                                                     GGTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGATCGTC             >  SRR4302540.6537411/1‑94 (MQ=60)
                                                                                      GTTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGATCGTCC            <  SRR4302540.2116269/94‑1 (MQ=60)
                                                                                       TTTGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGATCGTCCT           >  SRR4302540.6525875/1‑94 (MQ=60)
                                                                                         TGCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGATCGTCCTAT         <  SRR4302540.6569836/94‑1 (MQ=60)
                                                                                          GCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGATCGTCCTATT        <  SRR4302540.8294825/94‑1 (MQ=60)
                                                                                          GCA‑GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGATCGTCCTATT        <  SRR4302540.8294824/94‑1 (MQ=60)
                                                                                              GGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGATCGTCCTATTAGT     >  SRR4302540.9626488/1‑94 (MQ=60)
                                                                                              GGGGGGGATCGTTGAGATTGCCTCTCGTGTTGTCATTTACGTAAAAATCGTCAACCCCATGAGTCACCTGGAGCGCCCacatcgtcctgcgagt     >  SRR4302540.9626487/1‑78 (MQ=37)
                                                                                               GGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGATCGTCCTATTAGTC    >  SRR4302540.2938440/1‑94 (MQ=60)
                                                                                               GGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGATCGTCCTATTAGTC    <  SRR4302540.1551585/94‑1 (MQ=60)
                                                                                               GGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGGCAACCCCATGAGCCACCTGGTGCGCCCCGATCGTACTATTAGTC    >  SRR4302540.4570392/1‑94 (MQ=60)
                                                                                                GGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGATCGTCCTATTAGTCC   <  SRR4302540.2964093/94‑1 (MQ=60)
                                                                                                 GGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGATCGTCCTATTAGTCCA  >  SRR4302540.99512/1‑94 (MQ=60)
                                                                                                 |                                                                                             
GGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATA‑‑‑CAGAAACCTGCGGTTTGCA‑GGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGATCGTCCTATTAGTCCA  >  NC_000913/1924092‑1924278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑