Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,953,902 T→C Q377R (CAG→CGG)  b1872 ← biotin sulfoxide reductase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,953,9020TC99.6% 754.3 / ‑4.9 234Q377R (CAG→CGG) b1872biotin sulfoxide reductase 2
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (122/111);  total (122/112)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

Only 100 of 243 total aligned reads displayed.

ATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATT  >  NC_000913/1953812‑1953991
                                                                                          |                                                                                         
aTTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGc                                                                                         >  2:2739383/1‑93 (MQ=255)
 tttCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGcc                                                                                        >  1:6540681/1‑93 (MQ=255)
 tttCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAAACGCCGCACGGTGTACCAATCCGcc                                                                                        >  2:1251487/1‑93 (MQ=255)
  ttCCGGCAGCACGCCGCCAACACTCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGccc                                                                                       >  1:5831108/1‑93 (MQ=255)
   tCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCt                                                                                      <  2:1883298/93‑1 (MQ=255)
     cGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTaa                                                                                    >  1:6624499/1‑93 (MQ=255)
      ggCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAAc                                                                                   <  1:9357420/93‑1 (MQ=255)
       gcagcaCGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACa                                                                                  <  1:3211926/93‑1 (MQ=255)
        cagcaCGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACAt                                                                                 <  1:2958731/93‑1 (MQ=255)
        cagcaCGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACAt                                                                                 <  2:1356772/93‑1 (MQ=255)
         agcaCGCCGCCAACACGCGTCGGGTTGTCACCGTTGGAGTAATGATAAGAGAAACGAAAACCGCCGCCCGGTGTACAAATCCGCCCTAACATc                                                                                >  1:8739387/1‑93 (MQ=255)
         agcaCGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATc                                                                                <  1:3741586/93‑1 (MQ=255)
          gcaCGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATcg                                                                               >  2:7242014/1‑93 (MQ=255)
           caCGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATcgc                                                                              <  1:7163531/93‑1 (MQ=255)
           caCGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATcgc                                                                              <  1:5343890/93‑1 (MQ=255)
            aCGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATcgcc                                                                             >  2:3083090/1‑93 (MQ=255)
             cgccgcCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATcgccg                                                                            >  2:7801928/1‑93 (MQ=255)
               ccgccAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCgccgcc                                                                          <  2:642493/93‑1 (MQ=255)
                 gccAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAg                                                                        <  1:6844016/93‑1 (MQ=255)
                  ccAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGc                                                                       <  2:9902684/93‑1 (MQ=255)
                  ccAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGc                                                                       <  2:239426/93‑1 (MQ=255)
                   cAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCg                                                                      <  1:7456200/93‑1 (MQ=255)
                    aaCACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGt                                                                     >  2:2859980/1‑93 (MQ=255)
                     acacGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTc                                                                    <  2:789809/93‑1 (MQ=255)
                      cacGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCa                                                                   <  2:3014771/93‑1 (MQ=255)
                       acGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCAc                                                                  >  1:9168186/1‑93 (MQ=255)
                          cgTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAg                                                               >  1:3479883/1‑93 (MQ=255)
                           gTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGc                                                              >  1:5107310/1‑93 (MQ=255)
                           gTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGc                                                              <  1:609255/93‑1 (MQ=255)
                            tCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCa                                                             >  1:4998614/1‑93 (MQ=255)
                            tCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCa                                                             >  1:2233251/1‑93 (MQ=255)
                            tCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCa                                                             >  2:5175145/1‑93 (MQ=255)
                              gggTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATc                                                           <  2:4338714/93‑1 (MQ=255)
                              gggTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATc                                                           <  1:9791916/93‑1 (MQ=255)
                              gggTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATc                                                           >  2:4024863/1‑93 (MQ=255)
                               ggTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATcc                                                          >  2:804024/1‑93 (MQ=255)
                               ggTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATcc                                                          <  2:7091554/93‑1 (MQ=255)
                                gTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCa                                                         <  1:7738393/93‑1 (MQ=255)
                                 ttGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAg                                                        >  1:248774/1‑93 (MQ=255)
                                 ttGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAg                                                        <  2:7411349/93‑1 (MQ=255)
                                 ttGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAg                                                        >  1:9214049/1‑93 (MQ=255)
                                 ttGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAg                                                        <  1:1636084/93‑1 (MQ=255)
                                  tGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAgt                                                       <  2:2508764/93‑1 (MQ=255)
                                   gCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAgtg                                                      <  2:378405/93‑1 (MQ=255)
                                     cACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGtt                                                    <  2:8584234/93‑1 (MQ=255)
                                      cccGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGttt                                                   <  2:6148732/92‑1 (MQ=255)
                                      aCCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGttt                                                   >  2:2002410/1‑93 (MQ=255)
                                      aCCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGttt                                                   <  2:4926849/93‑1 (MQ=255)
                                      aCCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGttt                                                   <  1:5263031/93‑1 (MQ=255)
                                       ccGTTGGGGTAATGATAAGAGAAACCAAAACCCCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCCTCACCTGCCTCCAGTGTTTc                                                  >  2:6852567/1‑93 (MQ=255)
                                       ccGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTc                                                  <  2:8343650/93‑1 (MQ=255)
                                       ccGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTc                                                  <  1:7853598/93‑1 (MQ=255)
                                        cGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCt                                                 >  2:9733400/1‑93 (MQ=255)
                                         gTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTg                                                >  1:8134348/1‑93 (MQ=255)
                                            ggAGCAATCATAAGAGAAACCAAAACCGCCCCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTc                                             <  1:3102471/93‑1 (MQ=255)
                                               gTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCAcc                                          <  1:4232097/93‑1 (MQ=255)
                                                tAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCa                                         >  2:2680618/1‑93 (MQ=255)
                                                 aaTGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCat                                        >  2:146753/1‑93 (MQ=255)
                                                 aaTGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCat                                        >  1:2374206/1‑93 (MQ=255)
                                                  aTGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCata                                       >  2:2291219/1‑93 (MQ=255)
                                                   tGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCatat                                      >  2:1582776/1‑93 (MQ=255)
                                                   tGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCatat                                      >  2:3453910/1‑93 (MQ=255)
                                                    gaaaaGAGAAACCAAAACCGCCGCCCGGTGTACCAACCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATAtt                                     <  1:2144216/90‑1 (MQ=255)
                                                      tAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGc                                   <  2:1172773/93‑1 (MQ=255)
                                                       aaGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCt                                  >  1:3991359/1‑93 (MQ=255)
                                                          agaAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGgc                               <  2:5726195/93‑1 (MQ=255)
                                                          agaAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGgc                               >  1:8850118/1‑93 (MQ=255)
                                                          agaAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGgc                               <  1:9445686/93‑1 (MQ=255)
                                                           gaAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGgcg                              <  2:6802217/93‑1 (MQ=255)
                                                              aCCAAAACCGCCCCCCGGTCTACCAATCCGCCCTAACAGCGCCGCCAGCCTCACCAGCATCCAGTGTTTCTGTTCCCCATATTGCTGGCGCTg                           <  2:2051610/93‑1 (MQ=255)
                                                               ccAAAACCGCCGCCCGTTGTGCCAATCCGCCCTAACACCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGa                          <  1:964550/93‑1 (MQ=255)
                                                               ccAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGa                          >  1:1983449/1‑93 (MQ=255)
                                                               ccAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGa                          <  1:8326441/93‑1 (MQ=255)
                                                                cAAACCCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGaa                         <  2:1377793/93‑1 (MQ=255)
                                                                cAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGaa                         <  2:8859699/93‑1 (MQ=255)
                                                                 aaaaCCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAAt                        <  2:4549849/93‑1 (MQ=255)
                                                                 aaaaCCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAAt                        <  1:1174728/93‑1 (MQ=255)
                                                                   aaCCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTc                      <  1:9066615/93‑1 (MQ=255)
                                                                   aaCCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTc                      >  2:3430184/1‑93 (MQ=255)
                                                                   aaCCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTc                      >  1:2260104/1‑93 (MQ=255)
                                                                     ccgccgCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTccc                    >  2:6703538/1‑93 (MQ=255)
                                                                      cgccgcCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTcccc                   <  2:2566898/93‑1 (MQ=255)
                                                                        ccgccCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAg                 <  1:3308076/93‑1 (MQ=255)
                                                                          gccCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGcc               <  2:5814098/93‑1 (MQ=255)
                                                                           cccGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCt              >  1:4561428/1‑93 (MQ=255)
                                                                            ccGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTg             >  2:6954722/1‑93 (MQ=255)
                                                                            ccGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTg             >  1:4609785/1‑93 (MQ=255)
                                                                              ggTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGcc           >  1:2628835/1‑93 (MQ=255)
                                                                                tgtACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCAt         <  1:1236292/93‑1 (MQ=255)
                                                                                tgtACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCAt         >  2:10042744/1‑93 (MQ=255)
                                                                                 gtACCAACCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCGGCCATc        <  2:5575518/93‑1 (MQ=255)
                                                                                  tACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCa       <  1:4708688/93‑1 (MQ=255)
                                                                                  tACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCa       <  2:4077149/93‑1 (MQ=255)
                                                                                    ccAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGc     <  1:7550879/93‑1 (MQ=255)
                                                                                     cAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCa    <  1:5669534/93‑1 (MQ=255)
                                                                                     cAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCa    >  1:4333310/1‑93 (MQ=255)
                                                                                      aaTCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCAt   >  2:9104770/1‑93 (MQ=255)
                                                                                       aTCCGCCCTTACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCAtt  >  2:62780/1‑93 (MQ=255)
                                                                                       aTCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCAtt  <  1:683105/93‑1 (MQ=255)
                                                                                       aTCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCAtt  >  2:4452678/1‑93 (MQ=255)
                                                                                          |                                                                                         
ATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATT  >  NC_000913/1953812‑1953991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGC  >  NC_000913/1953812‑1953995
                                                                                          |                                                                                             
ATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCC                                                                                            >  SRR4302540.9757602/1‑94 (MQ=60)
ATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCC                                                                                            >  SRR4302540.6613262/1‑94 (MQ=60)
 TTTCCGGCAGCACGCCGCCAACACTCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCC                                                                                           >  SRR4302540.5895583/1‑94 (MQ=60)
  TTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCGCCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCT                                                                                          <  SRR4302540.701570/94‑1 (MQ=60)
   TCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTA                                                                                         <  SRR4302540.5316692/94‑1 (MQ=60)
    CCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAA                                                                                        >  SRR4302540.6697837/1‑94 (MQ=60)
     CGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAAC                                                                                       >  SRR4302540.593295/1‑94 (MQ=60)
      GGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACA                                                                                      <  SRR4302540.9468865/94‑1 (MQ=60)
       GCAGCACGCCGCCAACACGCGTCGGTTTGCCGCCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACAT                                                                                     <  SRR4302540.5983169/94‑1 (MQ=60)
       GCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACAT                                                                                     <  SRR4302540.3246793/94‑1 (MQ=60)
       GCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACAT                                                                                     >  SRR4302540.8359632/1‑94 (MQ=60)
        CAGCACGCCGCCAACACGCGTCGGGTTGTCACCGTTGGAGTAATGATAAGAGAAACGAAAACCGCCGCCCGGTGTACAAATCCGCCCTAACATC                                                                                    >  SRR4302540.8840842/1‑94 (MQ=60)
        CAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATC                                                                                    <  SRR4302540.2991335/94‑1 (MQ=60)
         AGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCG                                                                                   <  SRR4302540.3782411/94‑1 (MQ=60)
         AGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCG                                                                                   <  SRR4302540.1313777/94‑1 (MQ=60)
           CACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAACCCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCC                                                                                 >  SRR4302540.3554151/1‑94 (MQ=60)
           CACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCC                                                                                 <  SRR4302540.6914138/94‑1 (MQ=60)
           CACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCC                                                                                 <  SRR4302540.7243394/94‑1 (MQ=60)
           CACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCC                                                                                 <  SRR4302540.5402508/94‑1 (MQ=60)
            ACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCG                                                                                >  SRR4302540.7545334/1‑94 (MQ=60)
               CCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCA                                                                             >  SRR4302540.637604/1‑94 (MQ=60)
                CGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAG                                                                            >  SRR4302540.4638241/1‑94 (MQ=60)
                 GCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGC                                                                           <  SRR4302540.6919832/94‑1 (MQ=60)
                  CCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCG                                                                          >  SRR4302540.2347974/1‑94 (MQ=60)
                  CCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCG                                                                          >  SRR4302540.1986596/1‑94 (MQ=60)
                  CCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCG                                                                          >  SRR4302540.8116157/1‑94 (MQ=60)
                  CCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCG                                                                          >  SRR4302540.5595940/1‑94 (MQ=60)
                  CCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCG                                                                          >  SRR4302540.2347973/1‑94 (MQ=60)
                   CAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGT                                                                         <  SRR4302540.7539331/94‑1 (MQ=60)
                     ACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCA                                                                       <  SRR4302540.6632524/94‑1 (MQ=60)
                     ACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCA                                                                       >  SRR4302540.2256753/1‑94 (MQ=60)
                      CACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCAC                                                                      >  SRR4302540.9278178/1‑94 (MQ=60)
                         GCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAG                                                                   >  SRR4302540.3517775/1‑94 (MQ=60)
                          CGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGC                                                                  >  SRR4302540.5162596/1‑94 (MQ=60)
                           GTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCA                                                                 <  SRR4302540.615768/94‑1 (MQ=60)
                           GTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCA                                                                 >  SRR4302540.2257287/1‑94 (MQ=60)
                           GTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCA                                                                 >  SRR4302540.5052734/1‑94 (MQ=60)
                           GTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCA                                                                 >  SRR4302540.5052735/1‑94 (MQ=60)
                             CGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATC                                                               >  SRR4302540.4750158/1‑94 (MQ=60)
                              GGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCC                                                              <  SRR4302540.9906616/94‑1 (MQ=60)
                              GGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCC                                                              <  SRR4302540.9906615/94‑1 (MQ=60)
                              GGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCC                                                              >  SRR4302540.1837474/1‑94 (MQ=60)
                              GGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCC                                                              <  SRR4302540.9251501/94‑1 (MQ=60)
                                GTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAG                                                            <  SRR4302540.7828732/94‑1 (MQ=60)
                                GTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAG                                                            >  SRR4302540.9324596/1‑94 (MQ=60)
                                GTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAG                                                            >  SRR4302540.251184/1‑94 (MQ=60)
                                 TTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGT                                                           <  SRR4302540.1653978/94‑1 (MQ=60)
                                    CCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTT                                                        >  SRR4302540.686701/1‑94 (MQ=60)
                                     CACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTT                                                       >  SRR4302540.9460945/1‑94 (MQ=60)
                                     CACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGGCACCAGCATCCAGTGTTT                                                       <  SRR4302540.9284591/94‑1 (MQ=60)
                                      ACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTC                                                      <  SRR4302540.5320457/94‑1 (MQ=60)
                                      ACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATACAGTGTTTC                                                      >  SRR4302540.4191308/1‑94 (MQ=60)
                                       CCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACTAGCATCCAGTGTTTCT                                                     <  SRR4302540.8176627/94‑1 (MQ=60)
                                       CCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCT                                                     <  SRR4302540.7945360/94‑1 (MQ=60)
                                       CCGTGGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCT                                                     <  SRR4302540.5351222/94‑1 (MQ=60)
                                        CGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTG                                                    >  SRR4302540.8228757/1‑94 (MQ=60)
                                         GTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGT                                                   >  SRR4302540.217119/1‑94 (MQ=60)
                                           TGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTC                                                 >  SRR4302540.2825462/1‑94 (MQ=60)
                                            GGAGTAATGATAAGAACGACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCA                                                <  SRR4302540.9098340/94‑1 (MQ=60)
                                            GGAGCAATCATAAGAGAAACCAAAACCGCCCCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCA                                                <  SRR4302540.3136446/94‑1 (MQ=60)
                                              AGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACC                                              <  SRR4302540.5198399/94‑1 (MQ=60)
                                              AGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGACGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACC                                              >  SRR4302540.9596150/1‑94 (MQ=60)
                                               GTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCA                                             <  SRR4302540.4278160/94‑1 (MQ=60)
                                                TAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCAT                                            >  SRR4302540.3113706/1‑94 (MQ=60)
                                                TAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCAT                                            >  SRR4302540.2399787/1‑94 (MQ=60)
                                                 AATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATA                                           >  SRR4302540.5645342/1‑94 (MQ=60)
                                                 AATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATA                                           >  SRR4302540.20565/1‑94 (MQ=60)
                                                  ATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATAT                                          >  SRR4302540.2498653/1‑94 (MQ=60)
                                                   TGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATT                                         >  SRR4302540.7203850/1‑94 (MQ=60)
                                                   TGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATT                                         >  SRR4302540.3420577/1‑94 (MQ=60)
                                                    GAAAAGAGAAACCAAAACCGCCGCCCGGTGTACCAACCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTG                                        <  SRR4302540.2167260/94‑1 (MQ=60)
                                                     ATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGC                                       >  SRR4302540.8465712/1‑94 (MQ=60)
                                                     ATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGC                                       >  SRR4302540.9534788/1‑94 (MQ=60)
                                                      TAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCT                                      <  SRR4302540.10046041/94‑1 (MQ=60)
                                                      TAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCT                                      >  SRR4302540.3237849/1‑94 (MQ=60)
                                                      TAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCT                                      >  SRR4302540.4034317/1‑94 (MQ=60)
                                                        AGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGG                                    >  SRR4302540.4543930/1‑94 (MQ=60)
                                                         GAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGC                                   >  SRR4302540.8952501/1‑94 (MQ=60)
                                                          AGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCG                                  <  SRR4302540.9557821/94‑1 (MQ=60)
                                                           GAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGC                                 >  SRR4302540.6857345/1‑94 (MQ=60)
                                                              ACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGA                              >  SRR4302540.2004951/1‑94 (MQ=60)
                                                               CCAAAACCGCCGCCCGTTGTGCCAATCCGCCCTAACACCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAA                             <  SRR4302540.974510/94‑1 (MQ=60)
                                                               CCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAA                             <  SRR4302540.8423075/94‑1 (MQ=60)
                                                               CCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAA                             >  SRR4302540.3348377/1‑94 (MQ=60)
                                                                CAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAAT                            <  SRR4302540.2271852/94‑1 (MQ=60)
                                                                 AAAGCCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATT                           <  SRR4302540.9359236/94‑1 (MQ=60)
                                                                 AAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATT                           >  SRR4302540.8914279/1‑94 (MQ=60)
                                                                 AAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATT                           <  SRR4302540.1186869/94‑1 (MQ=60)
                                                                  AAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTC                          >  SRR4302540.8761210/1‑94 (MQ=60)
                                                                  AAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTC                          >  SRR4302540.2284350/1‑94 (MQ=60)
                                                                  AAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTC                          >  SRR4302540.78898/1‑94 (MQ=60)
                                                                   AACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCC                         <  SRR4302540.9175481/94‑1 (MQ=60)
                                                                     CCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCC                       <  SRR4302540.10081230/94‑1 (MQ=60)
                                                                      CGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCA                      >  SRR4302540.1241656/1‑94 (MQ=60)
                                                                       GCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAG                     <  SRR4302540.4707085/94‑1 (MQ=60)
                                                                       GCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAG                     >  SRR4302540.333210/1‑94 (MQ=60)
                                                                       CCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAG                     <  SRR4302540.9010427/94‑1 (MQ=60)
                                                                        CCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGC                    <  SRR4302540.3344317/94‑1 (MQ=60)
                                                                         CGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCC                   <  SRR4302540.7264881/94‑1 (MQ=60)
                                                                          GGCCGGTGTACCAGTCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCT                  <  SRR4302540.286671/94‑1 (MQ=60)
                                                                          GCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCT                  >  SRR4302540.4445025/1‑94 (MQ=60)
                                                                          GCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCT                  >  SRR4302540.4610710/1‑94 (MQ=60)
                                                                           CCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTG                 >  SRR4302540.4659273/1‑94 (MQ=60)
                                                                           CCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTG                 >  SRR4302540.4059920/1‑94 (MQ=60)
                                                                           CCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTG                 >  SRR4302540.7582431/1‑94 (MQ=60)
                                                                             CGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCC               >  SRR4302540.2657284/1‑94 (MQ=60)
                                                                             CGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCC               >  SRR4302540.3157351/1‑94 (MQ=60)
                                                                                TGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATC            <  SRR4302540.1248916/94‑1 (MQ=60)
                                                                                 GTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCA           >  SRR4302540.5053029/1‑94 (MQ=60)
                                                                                 GTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCA           >  SRR4302540.5830648/1‑94 (MQ=60)
                                                                                  TACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAG          <  SRR4302540.4296981/94‑1 (MQ=60)
                                                                                  TACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAG          >  SRR4302540.2575326/1‑94 (MQ=60)
                                                                                  TACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAG          <  SRR4302540.4759010/94‑1 (MQ=60)
                                                                                   ACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGC         >  SRR4302540.3980199/1‑94 (MQ=60)
                                                                                   ACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGC         <  SRR4302540.3621759/94‑1 (MQ=60)
                                                                                    CCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCA        >  SRR4302540.4380481/1‑94 (MQ=60)
                                                                                    CCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCA        <  SRR4302540.7635518/94‑1 (MQ=60)
                                                                                    CCAATCCACCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCA        <  SRR4302540.7635519/94‑1 (MQ=60)
                                                                                     CAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCAT       <  SRR4302540.5731963/94‑1 (MQ=60)
                                                                                       ATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTG     <  SRR4302540.690435/94‑1 (MQ=60)
                                                                                        TCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGG    >  SRR4302540.4876833/1‑94 (MQ=60)
                                                                                         CCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTG   <  SRR4302540.3781186/94‑1 (MQ=60)
                                                                                          CGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGC  <  SRR4302540.2148339/94‑1 (MQ=60)
                                                                                          CGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGC  >  SRR4302540.7416922/1‑94 (MQ=60)
                                                                                          |                                                                                             
ATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGC  >  NC_000913/1953812‑1953995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑