Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,969,062 T→C T552A (ACA→GCA)  tar ← methyl‑accepting chemotaxis protein II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,969,0620TC91.8% 716.9 / 44.5 270T552A (ACA→GCA) tarmethyl‑accepting chemotaxis protein II
Reads supporting (aligned to +/- strand):  ref base T (5/17);  new base C (127/121);  total (132/138)
Fisher's exact test for biased strand distribution p-value = 1.33e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.37e-01

Only 100 of 274 total aligned reads displayed.

AAAACAGCGTGGTCGAAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGTTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTATT  >  NC_000913/1968972‑1969152
                                                                                          |                                                                                          
   aCNGCGTGGTCGAAATTCGAANNCGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTccc                                                                                       <  1:1228899/93‑1 (MQ=255)
   aCAGCGTGGTCGAAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTccc                                                                                       >  2:1902779/1‑93 (MQ=255)
   aCAGCGTGGTCGAAATTCGAATACGATTAAACAGCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTccc                                                                                       <  1:1115661/93‑1 (MQ=255)
   aCAGCGCGCTCAAAAGTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTccc                                                                                       <  2:3584657/93‑1 (MQ=255)
    cAGCGTGGTCGAAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGTTTCCCa                                                                                      >  1:7031203/1‑93 (MQ=255)
    cAGCGTGGTCGAAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCa                                                                                      <  2:6172845/93‑1 (MQ=255)
     aGCGTGGTCGAAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAg                                                                                     <  2:5462960/93‑1 (MQ=255)
      gCGTGGTCGAAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGt                                                                                    >  1:4290951/1‑93 (MQ=255)
      gCGTGGTCGAAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGt                                                                                    <  1:1919956/93‑1 (MQ=255)
        gTGGTCGAAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGttt                                                                                  <  2:4269672/93‑1 (MQ=255)
           gTCGAAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGTTTCCCAGTTTGGa                                                                               <  1:9016293/93‑1 (MQ=255)
           gTCGAAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGa                                                                               >  2:7378814/1‑93 (MQ=255)
             cGAAATTCGAATACGATTATACATCAACGCTCCCCATCAGGCGACAATGTCCGCTTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATc                                                                             <  1:2588951/93‑1 (MQ=255)
             cGAAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATc                                                                             >  1:6824927/1‑93 (MQ=255)
              gAAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCt                                                                            >  2:5515627/1‑93 (MQ=255)
              gAAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCt                                                                            >  1:2258333/1‑93 (MQ=255)
              caaaTTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGTTTCCCAGTTTGGATCt                                                                            <  1:8405020/92‑1 (MQ=255)
                aaTTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTCTGGATCTTg                                                                          <  2:792543/93‑1 (MQ=255)
                 aTTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTCGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGt                                                                         >  2:576626/1‑93 (MQ=255)
                  ttCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGtt                                                                        >  1:4388954/1‑93 (MQ=255)
                    cGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCa                                                                      >  2:9891525/1‑93 (MQ=255)
                    cGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCa                                                                      <  1:8628953/93‑1 (MQ=255)
                    cGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCa                                                                      <  2:8793922/93‑1 (MQ=255)
                     gAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATGCTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAg                                                                     <  1:2807208/93‑1 (MQ=255)
                     gAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGTTTCCCAGTTTGGATCTTGTTCAg                                                                     <  2:7192143/93‑1 (MQ=255)
                     gAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAg                                                                     <  1:6770084/93‑1 (MQ=255)
                     gAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGGTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAg                                                                     <  2:4037470/93‑1 (MQ=255)
                      aaTACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGc                                                                    >  1:8167705/1‑93 (MQ=255)
                       aTACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCa                                                                   >  2:6420488/1‑93 (MQ=255)
                        tACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCaa                                                                  >  2:38936/1‑93 (MQ=255)
                        tACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCaa                                                                  <  2:519391/93‑1 (MQ=255)
                         aCGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAAt                                                                 <  2:1513788/93‑1 (MQ=255)
                         aCGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAAt                                                                 <  1:4123860/93‑1 (MQ=255)
                          cGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAAtt                                                                >  2:3712133/1‑93 (MQ=255)
                           gATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTc                                                               <  1:2406567/93‑1 (MQ=255)
                            aTTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCg                                                              >  1:6377802/1‑93 (MQ=255)
                            aTTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCg                                                              >  2:2572958/1‑93 (MQ=255)
                             ttAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGc                                                             >  1:7406753/1‑93 (MQ=255)
                             ttAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGc                                                             >  1:8573743/1‑93 (MQ=255)
                              tAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCa                                                            <  2:3210632/93‑1 (MQ=255)
                               aaaCATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAg                                                           <  1:8888896/93‑1 (MQ=255)
                               aaaCATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAg                                                           <  2:6344151/93‑1 (MQ=255)
                                aaCATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGt                                                          <  2:5211215/93‑1 (MQ=255)
                                 aCATCAACGCTCCGCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTc                                                         <  2:6745102/93‑1 (MQ=255)
                                  cATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCg                                                        >  1:5995524/1‑93 (MQ=255)
                                   aTCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGt                                                       <  2:7293832/93‑1 (MQ=255)
                                   aTCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGt                                                       <  1:7904830/93‑1 (MQ=255)
                                   aTCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGt                                                       <  2:5905929/93‑1 (MQ=255)
                                      aaCGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGc                                                    <  1:1451137/93‑1 (MQ=255)
                                      aaCGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGc                                                    <  2:7612879/93‑1 (MQ=255)
                                          cTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAg                                                <  2:940504/93‑1 (MQ=255)
                                            ccccATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGcc                                              >  2:904139/1‑93 (MQ=255)
                                               cATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCggt                                           <  1:4989927/93‑1 (MQ=255)
                                               cATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCggg                                           >  2:3482279/1‑92 (MQ=255)
                                                aTCAGGCGTCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCggtg                                          <  1:8260069/93‑1 (MQ=255)
                                                aTCAGGCGGCAATGACCGCGTTAGTAAATGCTCGTCAATATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCggtg                                          <  1:5344900/93‑1 (MQ=255)
                                                  cAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCggtggt                                        <  2:4945974/93‑1 (MQ=255)
                                                    ggcggcAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCggtgggtg                                      >  2:5643681/1‑93 (MQ=255)
                                                    ggcggcAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTg                                      >  1:3008006/1‑93 (MQ=255)
                                                     gcggcAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGc                                     >  2:3097106/1‑93 (MQ=255)
                                                     gcggcAATGACCGCGTTAGGAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGc                                     >  1:2205480/1‑93 (MQ=255)
                                                      cggcAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCt                                    >  1:9877420/1‑93 (MQ=255)
                                                       ggcAATGACCGCGTTAGTAAATACTCGTCAAAATGTTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTc                                   <  1:3864571/93‑1 (MQ=255)
                                                        gcAATGACCGCGTTAGTAAATACTCGTCAAAATGTTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCa                                  <  1:6564206/93‑1 (MQ=255)
                                                        gcAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCa                                  >  1:3475102/1‑93 (MQ=255)
                                                         cAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCAc                                 >  2:2727993/1‑93 (MQ=255)
                                                         cAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCAc                                 <  1:5221152/93‑1 (MQ=255)
                                                          aaTGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCAcg                                >  1:10023509/1‑92 (MQ=255)
                                                           aTGACCGCGTTAGTAAATACTCGTCAGAATCCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTg                               <  1:5033252/93‑1 (MQ=255)
                                                           aTGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTg                               <  1:7605741/93‑1 (MQ=255)
                                                            tGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTgg                              <  1:2030645/93‑1 (MQ=255)
                                                               ccGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAg                           <  1:4020809/93‑1 (MQ=255)
                                                                cgcgTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAgg                          >  1:6483305/1‑93 (MQ=255)
                                                                 gcgTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTTGCTGAGCCGGAGGGTGCTCACTGGCAGGa                         >  2:6298033/1‑93 (MQ=255)
                                                                 gcgTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGa                         <  2:1390472/93‑1 (MQ=255)
                                                                 gcgTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGa                         <  2:9221626/93‑1 (MQ=255)
                                                                 gcgTTAGTAAAAACGCGTCAAAATGCTTCCTAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCGCACTGGCAGGa                         <  2:8602947/93‑1 (MQ=255)
                                                                    ttAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACgg                      >  1:3241746/1‑93 (MQ=255)
                                                                     tAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACggg                     <  2:1143471/93‑1 (MQ=255)
                                                                     tAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACggg                     <  2:7772731/93‑1 (MQ=255)
                                                                      aGTAAATACTCGTCAAAATGTTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGa                    <  1:39944/93‑1 (MQ=255)
                                                                      aGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGa                    <  1:5775380/93‑1 (MQ=255)
                                                                      aGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGa                    <  1:5893650/93‑1 (MQ=255)
                                                                      aGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGa                    >  1:8074002/1‑93 (MQ=255)
                                                                       gTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGAt                   <  2:2004484/93‑1 (MQ=255)
                                                                       gTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGAt                   <  1:8339248/93‑1 (MQ=255)
                                                                        tAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATg                  >  1:7789695/1‑93 (MQ=255)
                                                                        tAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATg                  <  2:6675748/93‑1 (MQ=255)
                                                                         aaaTACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATgg                 <  2:8301193/93‑1 (MQ=255)
                                                                          aaTACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGgt                <  1:159805/93‑1 (MQ=255)
                                                                           aTACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGgtg               >  1:9344675/1‑93 (MQ=255)
                                                                            tACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGgtgt              <  1:2319700/93‑1 (MQ=255)
                                                                             aCTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGtt             <  2:1686637/93‑1 (MQ=255)
                                                                               tCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTg           >  1:8449202/1‑93 (MQ=255)
                                                                               tCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTg           <  1:9287032/93‑1 (MQ=255)
                                                                                 gTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCg         >  1:6605820/1‑93 (MQ=255)
                                                                                  tCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCgg        <  1:6794593/93‑1 (MQ=255)
                                                                                    aaaaTGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGGTGCTCACTGGCAGGACGGGATGGGGTTTGCgggt      >  1:7938912/1‑91 (MQ=255)
                                                                                      aaTGTTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTa    <  2:7031202/93‑1 (MQ=255)
                                                                                       aTGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTAt   >  1:3370986/1‑93 (MQ=255)
                                                                                          |                                                                                          
AAAACAGCGTGGTCGAAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGTTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTATT  >  NC_000913/1968972‑1969152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTAAAAACAGCGTGGTCGAAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGTTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTATTGGT  >  NC_000913/1968969‑1969155
                                                                                             |                                                                                             
TTAAAAACAGCGTGGTCGAAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGC                                                                                               >  SRR4302540.5926357/1‑94 (MQ=60)
TTAAAAACAGCGTGGTCGAAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGC                                                                                               >  SRR4302540.5926358/1‑94 (MQ=60)
ATAAAAACAGCGTGGTCGAAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGC                                                                                               <  SRR4302540.2996614/94‑1 (MQ=60)
   AAAACAGCGTGGTCGAAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTC                                                                                            <  SRR4302540.2273701/94‑1 (MQ=60)
     AACAGCGTGGTCGAAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCC                                                                                          <  SRR4302540.8833569/94‑1 (MQ=60)
      ACNGCGTGGTCGAAATTCGAANNCGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCA                                                                                         <  SRR4302540.1241456/94‑1 (MQ=60)
      ACAGCGTGGTCGAAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGTTTCCCA                                                                                         >  SRR4302540.7109341/1‑94 (MQ=60)
      ACAGCGTGGTCGAAATTCGAATACGATTAAACAGCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCA                                                                                         <  SRR4302540.1127220/94‑1 (MQ=60)
        AGCGTGGTCGAAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGT                                                                                       >  SRR4302540.3711950/1‑94 (MQ=60)
        AGCGTGGTCGAAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGT                                                                                       >  SRR4302540.4337633/1‑94 (MQ=60)
         GCGTGGTCGAAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGTTTCCCAGTT                                                                                      <  SRR4302540.9893742/94‑1 (MQ=60)
         GCGTGGTCGAAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTT                                                                                      <  SRR4302540.1940904/94‑1 (MQ=60)
         GCGTGGTCGAAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTT                                                                                      >  SRR4302540.3487954/1‑94 (MQ=60)
         GCGTGGTCGAAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTT                                                                                      >  SRR4302540.8251414/1‑94 (MQ=60)
            TGGTCGAAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGG                                                                                   >  SRR4302540.4150654/1‑94 (MQ=60)
             GGTCGAAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGA                                                                                  >  SRR4302540.5260950/1‑94 (MQ=60)
              GTCGAAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGTTTCCCAGTTTGGAT                                                                                 <  SRR4302540.9124480/94‑1 (MQ=60)
               TCGAAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATC                                                                                >  SRR4302540.6900485/1‑94 (MQ=60)
                CGAAATTCGAATACGATTATACATCAACGCTCCCCATCAGGCGACAATGTCCGCTTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCT                                                                               <  SRR4302540.2616864/94‑1 (MQ=60)
                CGAAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCT                                                                               <  SRR4302540.7064490/94‑1 (MQ=60)
                CGAAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCT                                                                               <  SRR4302540.8573021/94‑1 (MQ=60)
                CGAAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCT                                                                               >  SRR4302540.2282572/1‑94 (MQ=60)
                 GAAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTT                                                                              >  SRR4302540.686752/1‑94 (MQ=60)
                 GAAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTT                                                                              >  SRR4302540.9123863/1‑94 (MQ=60)
                 GAAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTT                                                                              >  SRR4302540.6010239/1‑94 (MQ=60)
                 CAAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGTTTCCCAGTTTGGATCTT                                                                              <  SRR4302540.8502577/94‑1 (MQ=60)
                  AAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGTTTCCCAGTTTGGATCTTG                                                                             <  SRR4302540.3233121/94‑1 (MQ=60)
                  AAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTG                                                                             >  SRR4302540.7747486/1‑94 (MQ=60)
                    ATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTT                                                                           <  SRR4302540.5955732/94‑1 (MQ=60)
                    ATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTT                                                                           >  SRR4302540.6899554/1‑94 (MQ=60)
                    ATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTT                                                                           >  SRR4302540.4436588/1‑94 (MQ=60)
                    ATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTT                                                                           >  SRR4302540.1097541/1‑94 (MQ=60)
                       CGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAG                                                                        <  SRR4302540.8729183/94‑1 (MQ=60)
                       CAAATACGATTAAACATCAACGCTCCCCAGCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAG                                                                        <  SRR4302540.8729182/94‑1 (MQ=60)
                        GAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATGCTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGC                                                                       <  SRR4302540.2837527/94‑1 (MQ=60)
                        GAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGC                                                                       >  SRR4302540.8262553/1‑94 (MQ=60)
                        GAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGC                                                                       >  SRR4302540.9102399/1‑94 (MQ=60)
                        GAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGC                                                                       <  SRR4302540.6844903/94‑1 (MQ=60)
                          ATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAA                                                                     >  SRR4302540.679825/1‑94 (MQ=60)
                          ATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAA                                                                     >  SRR4302540.9305283/1‑94 (MQ=60)
                          ATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAA                                                                     >  SRR4302540.4039541/1‑94 (MQ=60)
                            ACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATT                                                                   <  SRR4302540.4168398/94‑1 (MQ=60)
                             CGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTC                                                                  <  SRR4302540.10080729/94‑1 (MQ=60)
                              GATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCG                                                                 >  SRR4302540.2921682/1‑94 (MQ=60)
                              GATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCG                                                                 >  SRR4302540.6448809/1‑94 (MQ=60)
                              GATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCG                                                                 <  SRR4302540.2432514/94‑1 (MQ=60)
                              GATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTCCCCAGTTTGGATCTTGTTCAGCAATTCG                                                                 <  SRR4302540.2402837/94‑1 (MQ=60)
                               ATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGC                                                                >  SRR4302540.8673357/1‑94 (MQ=60)
                               ATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGC                                                                >  SRR4302540.7489263/1‑94 (MQ=60)
                                 TAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAG                                                              <  SRR4302540.6331571/94‑1 (MQ=60)
                                 TAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAG                                                              <  SRR4302540.6452608/94‑1 (MQ=60)
                                 TAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAG                                                              <  SRR4302540.6331570/94‑1 (MQ=60)
                                 TAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAG                                                              <  SRR4302540.3405348/94‑1 (MQ=60)
                                 TAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAG                                                              <  SRR4302540.3405055/94‑1 (MQ=60)
                                 TAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAG                                                              <  SRR4302540.8380119/94‑1 (MQ=60)
                                  AAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGT                                                             <  SRR4302540.8991796/94‑1 (MQ=60)
                                  AAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGT                                                             >  SRR4302540.8306217/1‑94 (MQ=60)
                                  AAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGT                                                             <  SRR4302540.2342378/94‑1 (MQ=60)
                                    ACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCG                                                           >  SRR4302540.6061956/1‑94 (MQ=60)
                                     CATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGT                                                          <  SRR4302540.2388817/94‑1 (MQ=60)
                                      CTCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTG                                                         <  SRR4302540.7997021/94‑1 (MQ=60)
                                      ATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTG                                                         >  SRR4302540.2073832/1‑94 (MQ=60)
                                      ATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTG                                                         <  SRR4302540.5139777/94‑1 (MQ=60)
                                      ATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTG                                                         <  SRR4302540.7997020/94‑1 (MQ=60)
                                      ATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTG                                                         >  SRR4302540.6868950/1‑94 (MQ=60)
                                      ATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTG                                                         <  SRR4302540.1316130/94‑1 (MQ=60)
                                      ATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTG                                                         <  SRR4302540.4190705/94‑1 (MQ=60)
                                       TCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGG                                                        <  SRR4302540.6016340/94‑1 (MQ=60)
                                         AACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCT                                                      <  SRR4302540.1466913/94‑1 (MQ=60)
                                           CGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGA                                                    >  SRR4302540.117619/1‑94 (MQ=60)
                                           CGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGA                                                    >  SRR4302540.117618/1‑94 (MQ=60)
                                             CTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGC                                                  >  SRR4302540.8065374/1‑94 (MQ=60)
                                              TCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCG                                                 >  SRR4302540.6842310/1‑94 (MQ=60)
                                              TCCCCAGCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCC                                                 <  SRR4302540.4174066/94‑1 (MQ=60)
                                                 CCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGT                                              >  SRR4302540.4344926/1‑94 (MQ=60)
                                                  CATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTG                                             >  SRR4302540.5944132/1‑94 (MQ=60)
                                                  CATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTG                                             <  SRR4302540.8503510/94‑1 (MQ=60)
                                                  CATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTG                                             <  SRR4302540.5043954/94‑1 (MQ=60)
                                                  CATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTG                                             >  SRR4302540.8364463/1‑94 (MQ=60)
                                                   ATCAGGCGTCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGG                                            <  SRR4302540.8356056/94‑1 (MQ=60)
                                                   ATCAGGCGGCAATGACCGCGTTAGTAAATGCTCGTCAATATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGG                                            <  SRR4302540.5403532/94‑1 (MQ=60)
                                                   ATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGG                                            <  SRR4302540.1592426/94‑1 (MQ=60)
                                                   ATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGG                                            >  SRR4302540.5291404/1‑94 (MQ=60)
                                                    TCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGTTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGT                                           <  SRR4302540.296977/94‑1 (MQ=60)
                                                      AGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTG                                         >  SRR4302540.3041111/1‑94 (MQ=60)
                                                       GGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGC                                        >  SRR4302540.8460671/1‑94 (MQ=60)
                                                       GGCGGCAATGACCGCGTTAGGAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGC                                        >  SRR4302540.2229308/1‑94 (MQ=60)
                                                        GCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCT                                       >  SRR4302540.9992776/1‑94 (MQ=60)
                                                        GCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCT                                       >  SRR4302540.139527/1‑94 (MQ=60)
                                                         CGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTC                                      <  SRR4302540.5678773/94‑1 (MQ=60)
                                                          GGCAATGACCGCGTTAGTAAATACTCGTCAAAATGTTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCA                                     <  SRR4302540.3906577/94‑1 (MQ=60)
                                                          GGCAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCA                                     >  SRR4302540.3512942/1‑94 (MQ=60)
                                                           GCAATGACCGCGTTAGTAAATACTCGTCAAAATGTTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCAC                                    <  SRR4302540.6636955/94‑1 (MQ=60)
                                                           GCAATGACCGCGTTAGTAAATACTCGTCAAAATGTTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCAC                                    <  SRR4302540.6636956/94‑1 (MQ=60)
                                                            CAATGACCGCGTTAGTAAATACTCGTCAAAATGTTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACT                                   <  SRR4302540.3977861/94‑1 (MQ=60)
                                                            CAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCTGTGGTTGCTCACT                                   >  SRR4302540.8505200/1‑94 (MQ=60)
                                                            CAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACT                                   <  SRR4302540.5278073/94‑1 (MQ=60)
                                                            CAATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACG                                   >  SRR4302540.10140143/1‑94 (MQ=60)
                                                             AATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTG                                  <  SRR4302540.2006536/94‑1 (MQ=60)
                                                             AATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTG                                  >  SRR4302540.2519966/1‑94 (MQ=60)
                                                             AATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTG                                  >  SRR4302540.825344/1‑94 (MQ=60)
                                                              ATGACCGCGTTAGTAAATACTCGTCAGAATCCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGG                                 <  SRR4302540.5087710/94‑1 (MQ=60)
                                                              ATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGG                                 <  SRR4302540.7691209/94‑1 (MQ=60)
                                                              ATGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGG                                 <  SRR4302540.8002822/94‑1 (MQ=60)
                                                               TGACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGC                                <  SRR4302540.2052563/94‑1 (MQ=60)
                                                                 acctgGTTAGTAAATGCTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAG                              <  SRR4302540.7895549/89‑1 (MQ=60)
                                                                 ACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAG                              >  SRR4302540.2317151/1‑94 (MQ=60)
                                                                 ACCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAG                              <  SRR4302540.7895251/94‑1 (MQ=60)
                                                                  CCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGG                             >  SRR4302540.6555416/1‑94 (MQ=60)
                                                                  CCGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGG                             <  SRR4302540.4064133/94‑1 (MQ=60)
                                                                   CGCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGG                            >  SRR4302540.1943580/1‑94 (MQ=60)
                                                                    GCGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGAC                           <  SRR4302540.4046234/94‑1 (MQ=60)
                                                                     CGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACG                          <  SRR4302540.6986689/94‑1 (MQ=60)
                                                                     CGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACG                          >  SRR4302540.937576/1‑94 (MQ=60)
                                                                     CGTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACG                          >  SRR4302540.7761030/1‑94 (MQ=60)
                                                                      GTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGG                         >  SRR4302540.8292953/1‑94 (MQ=60)
                                                                      GTTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGG                         >  SRR4302540.3277028/1‑94 (MQ=60)
                                                                       TTAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGG                        <  SRR4302540.5305523/94‑1 (MQ=60)
                                                                        TAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGA                       >  SRR4302540.8167691/1‑94 (MQ=60)
                                                                        TAGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGA                       >  SRR4302540.161874/1‑94 (MQ=60)
                                                                         AGTAAATACTCGTCAAAATGTTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGAT                      <  SRR4302540.40356/94‑1 (MQ=60)
                                                                         AGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGAT                      <  SRR4302540.5839180/94‑1 (MQ=60)
                                                                         AGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGAT                      <  SRR4302540.5958747/94‑1 (MQ=60)
                                                                         AGTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGAT                      >  SRR4302540.39878/1‑94 (MQ=60)
                                                                          GTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATG                     >  SRR4302540.7880748/1‑94 (MQ=60)
                                                                          GTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATG                     <  SRR4302540.939119/94‑1 (MQ=60)
                                                                          GTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATG                     >  SRR4302540.6673072/1‑94 (MQ=60)
                                                                          GTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATG                     >  SRR4302540.4997738/1‑94 (MQ=60)
                                                                          GTAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATG                     <  SRR4302540.8436046/94‑1 (MQ=60)
                                                                           TAAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGG                    <  SRR4302540.6608484/94‑1 (MQ=60)
                                                                            AAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGT                   <  SRR4302540.3159124/94‑1 (MQ=60)
                                                                            AAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGT                   <  SRR4302540.7476515/94‑1 (MQ=60)
                                                                            AAATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGG                   >  SRR4302540.7963926/1‑94 (MQ=60)
                                                                             AATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTG                  >  SRR4302540.9456004/1‑94 (MQ=60)
                                                                             AATACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTG                  <  SRR4302540.161415/94‑1 (MQ=60)
                                                                              ATACTCGTCAAAATGTTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGT                 >  SRR4302540.4878511/1‑94 (MQ=60)
                                                                               TACTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTT                <  SRR4302540.2344674/94‑1 (MQ=60)
                                                                                 CTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTG              >  SRR4302540.5064037/1‑94 (MQ=60)
                                                                                 CTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTG              >  SRR4302540.8547293/1‑94 (MQ=60)
                                                                                 CTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGATTGCTCACTTGCAGGGCGGGGTGGTGATTG              >  SRR4302540.7295927/1‑94 (MQ=60)
                                                                                 CTCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGACGGCGGTTGCTCACTGGCAGGACGGGATGGAGATTG              >  SRR4302540.868716/1‑94 (MQ=60)
                                                                                  TCGTCAAAATGTTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGC             >  SRR4302540.4351865/1‑94 (MQ=60)
                                                                                  TCGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGC             <  SRR4302540.9398012/94‑1 (MQ=60)
                                                                                  TCGACAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGC             <  SRR4302540.3727181/94‑1 (MQ=60)
                                                                                   CGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCG            >  SRR4302540.6678966/1‑94 (MQ=60)
                                                                                   CGTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCG            >  SRR4302540.6678967/1‑94 (MQ=60)
                                                                                    GTCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGGTCACTGGCAGGACGGGATTGTGTTTGCGG           >  SRR4302540.7640087/1‑94 (MQ=60)
                                                                                     TCAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGT          <  SRR4302540.6869734/94‑1 (MQ=60)
                                                                                      CAAAATGTTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTT         <  SRR4302540.2442728/94‑1 (MQ=60)
                                                                                      CAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTT         >  SRR4302540.1647613/1‑94 (MQ=60)
                                                                                      CAAAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGGTGCTCACTGGCAGGACGGGATGGGGTTTGCGGGT         >  SRR4302540.8031433/1‑94 (MQ=60)
                                                                                        AAATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTT       >  SRR4302540.2717096/1‑94 (MQ=60)
                                                                                         AATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTAT      <  SRR4302540.8645991/94‑1 (MQ=60)
                                                                                         AATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTAT      >  SRR4302540.3407837/1‑94 (MQ=60)
                                                                                          ATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTTTT     >  SRR4302540.3038453/1‑94 (MQ=60)
                                                                                          ATGCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTATT     <  SRR4302540.9910044/94‑1 (MQ=60)
                                                                                           TGTTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTATTG    <  SRR4302540.2108098/94‑1 (MQ=60)
                                                                                            GCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTATTGG   <  SRR4302540.1683084/94‑1 (MQ=60)
                                                                                            GCTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTATTGG   <  SRR4302540.813178/94‑1 (MQ=60)
                                                                                             CTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGTGGTTTATTGGT  <  SRR4302540.2019022/94‑1 (MQ=60)
                                                                                             CTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGAGGGTTATTGGT  >  SRR4302540.7236552/1‑94 (MQ=60)
                                                                                             |                                                                                             
TTAAAAACAGCGTGGTCGAAATTCGAATACGATTAAACATCAACGCTCCCCATCAGGCGGCAATGACCGCGTTAGTAAATACTCGTCAAAATGTTTCCCAGTTTGGATCTTGTTCAGCAATTCGCAGTCGTGGCTGAGCCGGTGGTTGCTCACTGGCAGGACGGGATGGTGTTTGCGGTTTATTGGT  >  NC_000913/1968969‑1969155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑