Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,972,288	G→A	T354M (ACG→ATG)	cheA ←	CheA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,972,288	0	G	A	98.8%	856.1 / ‑2.8	241	T354M (ACG→ATG)	cheA	CheA
Reads supporting (aligned to +/- strand): ref base G (1/2); new base A (109/128); total (110/131)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01

Only 100 of 255 total aligned reads displayed.

TGATCGAGGCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCGTCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATGCGA  >  NC_000913/1972198‑1972377

tGATCGAGGCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATc                                                                                         >  2:5014940/1‑93 (MQ=255)

tGATCGAGGCTATTGCGTACCAGATGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATc                                                                                         <  2:6891030/93‑1 (MQ=255)

 gATCGATGCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCa                                                                                        <  2:4021747/93‑1 (MQ=255)

 gATCGAGGCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCa                                                                                        >  1:8416797/1‑93 (MQ=255)

  gtCGAGGGTATTTCGTACCAGGTTGGTCAGCGGGTTCATAATGCGTTCTATCAGGCTTTTGTCGGGTTCAGTAGAACTGCCCACCAGCATCAg                                                                                       <  1:2182692/92‑1 (MQ=255)

   tCGAGGCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCGTCAGt                                                                                      >  2:4735768/1‑93 (MQ=255)

   tCGAGGCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGt                                                                                      <  1:5135492/93‑1 (MQ=255)

    cGAGGCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGtt                                                                                     >  1:2657285/1‑93 (MQ=255)

     gaagCTACTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCATTTc                                                                                    <  1:6637995/90‑1 (MQ=255)

     gAGGCTATTGCGTCCCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTc                                                                                    <  2:2426634/93‑1 (MQ=255)

     gAGGCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTc                                                                                    <  2:9931249/93‑1 (MQ=255)

       ggCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTa                                                                                  >  2:10041525/1‑93 (MQ=255)

        gCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTAc                                                                                 <  2:5958490/93‑1 (MQ=255)

        gCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTAc                                                                                 >  2:3450133/1‑93 (MQ=255)

        gCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTAc                                                                                 <  2:7695539/93‑1 (MQ=255)

        gCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTAc                                                                                 >  1:6029347/1‑93 (MQ=255)

          tATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCt                                                                               <  1:6319471/93‑1 (MQ=255)

           aTTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTg                                                                              >  1:3586931/1‑93 (MQ=255)

           aTTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTg                                                                              >  1:3958230/1‑93 (MQ=255)

             tGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCt                                                                            <  2:3407670/93‑1 (MQ=255)

                 tACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGcc                                                                        >  1:9604952/1‑93 (MQ=255)

                   ccAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGa                                                                      >  1:6998340/1‑93 (MQ=255)

                    cAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAg                                                                     >  1:6895956/1‑93 (MQ=255)

                      ggTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGtt                                                                   >  1:860649/1‑93 (MQ=255)

                      ggTGGGTAAGCGGGTCGATGATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGtt                                                                   <  1:4141809/93‑1 (MQ=255)

                       gTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGttt                                                                  <  2:6240583/93‑1 (MQ=255)

                         gggTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTc                                                                >  2:6605281/1‑93 (MQ=255)

                         gggTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTc                                                                >  2:857729/1‑93 (MQ=255)

                          ggTGAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACGGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTcc                                                               <  2:4244948/93‑1 (MQ=255)

                          ggTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTcc                                                               >  2:165686/1‑93 (MQ=255)

                          ggTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTcc                                                               <  1:4163814/93‑1 (MQ=255)

                           gTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTccc                                                              <  1:1142778/93‑1 (MQ=255)

                           gTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTccc                                                              <  1:7855038/93‑1 (MQ=255)

                           gTCAGCGGGTCGATAATGCGTGCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTccc                                                              <  1:1941769/93‑1 (MQ=255)

                            tCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCg                                                             >  2:8258227/1‑93 (MQ=255)

                             cAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGc                                                            >  1:6608123/1‑93 (MQ=255)

                               gCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCa                                                          >  1:3742775/1‑93 (MQ=255)

                                 gggTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGa                                                        <  2:5805144/93‑1 (MQ=255)

                                 gggTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGa                                                        >  1:2531873/1‑93 (MQ=255)

                                 gggTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGa                                                        >  2:7189505/1‑93 (MQ=255)

                                  ggTCGATAATGCGTTCTATCAGGCTGTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTGTTCCCGCCAGAt                                                       <  2:6011987/93‑1 (MQ=255)

                                   gttgATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATc                                                      >  1:23322/4‑93 (MQ=255)

                                   gTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTCGCAATGCCCACCAGCCTCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGAta                                                      <  2:8990372/93‑2 (MQ=255)

                                   gTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATc                                                      <  1:2952508/93‑1 (MQ=255)

                                    tCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCa                                                     <  1:660222/93‑1 (MQ=255)

                                    tCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCa                                                     >  2:5220195/1‑93 (MQ=255)

                                      gATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACg                                                   >  1:5058978/1‑93 (MQ=255)

                                        tAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGcc                                                 >  1:4649624/1‑92 (MQ=255)

                                        tAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCa                                                 >  1:886203/1‑93 (MQ=255)

                                        tAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCa                                                 <  2:8499480/93‑1 (MQ=255)

                                             cGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGc                                            <  2:4552665/93‑1 (MQ=255)

                                              gTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCCCGCACCAGcc                                           >  2:9794531/1‑93 (MQ=255)

                                               ttCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCg                                          >  2:4880978/1‑93 (MQ=255)

                                                 cTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCGGCATCAGTTGTACCTGCTTGCCGAGGTTTCGCGCCAGATCACGAAACAGGCggg                                        >  1:1102320/1‑93 (MQ=255)

                                                 cTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCggg                                        <  2:7308765/93‑1 (MQ=255)

                                                 cTATCAGGCTTTGGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCggg                                        <  1:7458897/93‑1 (MQ=255)

                                                  tATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCgggg                                       <  1:5547062/93‑1 (MQ=255)

                                                   aTCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGa                                      <  2:452543/93‑1 (MQ=255)

                                                   aTCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGa                                      <  2:7369314/93‑1 (MQ=255)

                                                   aTCAGGCTTTTGGCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGa                                      <  1:7094862/93‑1 (MQ=255)

                                                    acAGGCTTTTCTCGAGTTGAGTAGATCTGCCCAGCAGCATCAGTTCTGCCTGCTTGCCGAGTTTTCCCGCCAGATCACGCGCCAGCCGGGGAt                                     <  2:6756919/92‑1 (MQ=255)

                                                      aGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAg                                   >  1:9045272/1‑93 (MQ=255)

                                                      aGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGCGATAg                                   >  1:8786228/1‑93 (MQ=255)

                                                         cTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGa                                <  2:7266883/93‑1 (MQ=255)

                                                         cTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGa                                >  1:8287860/1‑93 (MQ=255)

                                                          ttttGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGAc                               <  2:9715048/93‑1 (MQ=255)

                                                          ttttGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGAc                               <  2:2090553/93‑1 (MQ=255)

                                                           tttGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACt                              <  1:9144185/93‑1 (MQ=255)

                                                            ttGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTa                             >  1:3246276/1‑93 (MQ=255)

                                                            ttGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTa                             >  1:8029127/1‑93 (MQ=255)

                                                               tCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTaaaa                          <  1:4499813/93‑1 (MQ=255)

                                                                cGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTaaaaa                         >  2:2729510/1‑93 (MQ=255)

                                                                 gAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAAc                        <  2:2995213/93‑1 (MQ=255)

                                                                   gTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACat                      <  2:2510668/93‑1 (MQ=255)

                                                                   gTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACat                      >  2:8733266/1‑93 (MQ=255)

                                                                   gTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACat                      <  1:7608261/93‑1 (MQ=255)

                                                                    ttCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACata                     <  2:5490057/93‑1 (MQ=255)

                                                                    ttCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACata                     <  1:2602844/93‑1 (MQ=255)

                                                                    ttCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACata                     <  1:7220063/93‑1 (MQ=255)

                                                                     tCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACatat                    <  1:8148641/93‑1 (MQ=255)

                                                                       cgTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTc                  <  1:97137/92‑1 (MQ=255)

                                                                       aGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTc                  <  1:1414691/93‑1 (MQ=255)

                                                                        gTAGAACTGCCCCCCCGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTcc                 <  2:7717111/93‑1 (MQ=255)

                                                                        gTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTcc                 >  2:642355/1‑93 (MQ=255)

                                                                          aGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCGCCAGCCGGGGATAGCGACTAAAAACATATTCCAt               <  2:9395775/93‑1 (MQ=255)

                                                                            aaCTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCg             >  2:6294166/1‑93 (MQ=255)

                                                                              cTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGc           >  1:5505106/1‑93 (MQ=255)

                                                                                 cccACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGcatc        <  1:3479075/93‑1 (MQ=255)

                                                                                 cccACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGcatc        <  2:1778942/93‑1 (MQ=255)

                                                                                 cccACAAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGcatc        <  1:4329066/93‑1 (MQ=255)

                                                                                  ccaccaGGATCTGTAATACCTGCTTGGCGAGTTTTCCCGCCAGATCGCGCTCCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGcatca       <  2:7818216/93‑1 (MQ=255)

                                                                                  ccaccaGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGcatca       >  2:3029613/1‑93 (MQ=255)

                                                                                  ccaccaGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGcatca       <  2:4171087/93‑1 (MQ=255)

                                                                                   caccaGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGcatcat      <  1:1292777/93‑1 (MQ=255)

                                                                                    accaGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATg     <  1:1790270/93‑1 (MQ=255)

                                                                                    accaGCATCAGTTCTACCTGCTTGCCGAGTTTTACCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATg     >  2:7503024/1‑93 (MQ=255)

                                                                                     ccaGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATGc    <  1:8318975/93‑1 (MQ=255)

                                                                                     ccaGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATGc    >  2:1288229/1‑93 (MQ=255)

                                                                                      caGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATGCg   >  2:7905620/1‑93 (MQ=255)

                                                                                       aGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATGCGa  >  2:3822949/1‑93 (MQ=255)

TGATCGAGGCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCGTCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATGCGA  >  NC_000913/1972198‑1972377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGTGATCGAGGCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCGTCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATGCGAATCG  >  NC_000913/1972196‑1972381

CGTGATCGAGGCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGATCAGTAGAACTGCCCACCAGCAT                                                                                              <  SRR4302540.5919511/94‑1 (MQ=60)

  TGATCGAGGCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCA                                                                                            >  SRR4302540.8514495/1‑94 (MQ=60)

   GATCGAGGCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAG                                                                                           >  SRR4302540.43894/1‑94 (MQ=60)

    GTCGAGGGTATTTCGTACCAGGTTGGTCAGCGGGTTCATAATGCGTTCTATCAGGCTTTTGTCGGGTTCAGTAGAACTGCCCACCAGCATCAGT                                                                                          <  SRR4302540.2206246/94‑1 (MQ=60)

    ATCGAGGCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCGTCAGT                                                                                          <  SRR4302540.128448/94‑1 (MQ=60)

    ATCGAGGCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGT                                                                                          <  SRR4302540.5010039/94‑1 (MQ=60)

    ATCGAGGCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGGTCAGTAGAACTGCCCACCAGCATCAGT                                                                                          >  SRR4302540.8997362/1‑94 (MQ=60)

     TCGAGGCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTT                                                                                         >  SRR4302540.2686125/1‑94 (MQ=60)

     TCGAGGCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTT                                                                                         <  SRR4302540.5191115/94‑1 (MQ=60)

       GAGGCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCT                                                                                       <  SRR4302540.786112/94‑1 (MQ=60)

       GAAGCTACTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCATTTCT                                                                                       <  SRR4302540.6711453/94‑1 (MQ=60)

         GGCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTAC                                                                                     >  SRR4302540.5530857/1‑94 (MQ=60)

         GGCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTAC                                                                                     >  SRR4302540.6096255/1‑94 (MQ=60)

          atctggaacttCCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACC                                                                                    <  SRR4302540.3310463/83‑1 (MQ=60)

          GCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACC                                                                                    <  SRR4302540.4452481/94‑1 (MQ=60)

            TATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTG                                                                                  >  SRR4302540.3626107/1‑94 (MQ=60)

            TATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTG                                                                                  >  SRR4302540.4000929/1‑94 (MQ=60)

            TATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTG                                                                                  <  SRR4302540.6389844/94‑1 (MQ=60)

            TATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTG                                                                                  <  SRR4302540.6711347/94‑1 (MQ=60)

            TATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTG                                                                                  <  SRR4302540.1858722/94‑1 (MQ=60)

            TATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGGCGAGGTCAGTAGAACTGCCCCCCAGCATCAGTTCTACCTG                                                                                  >  SRR4302540.4000930/1‑94 (MQ=60)

               TGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTT                                                                               <  SRR4302540.5730411/94‑1 (MQ=60)

                  GTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCC                                                                            <  SRR4302540.8397077/94‑1 (MQ=60)

                  GTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCC                                                                            <  SRR4302540.866525/94‑1 (MQ=60)

                  GTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCC                                                                            >  SRR4302540.9718233/1‑94 (MQ=60)

                   TACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCG                                                                           >  SRR4302540.2091420/1‑94 (MQ=60)

                    ACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGA                                                                          >  SRR4302540.7076008/1‑94 (MQ=60)

                     CCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAG                                                                         <  SRR4302540.1153080/94‑1 (MQ=60)

                     CCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAG                                                                         >  SRR4302540.6972490/1‑94 (MQ=60)

                       AGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTT                                                                       >  SRR4302540.869787/1‑94 (MQ=60)

                        GGTGGGTAAGCGGGTCGATGATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTT                                                                      <  SRR4302540.4186622/94‑1 (MQ=60)

                          TGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTC                                                                    >  SRR4302540.1424121/1‑94 (MQ=60)

                            GGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCC                                                                  <  SRR4302540.4208946/94‑1 (MQ=60)

                            GGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCC                                                                  >  SRR4302540.1660867/1‑94 (MQ=60)

                             GTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCG                                                                 <  SRR4302540.1154720/94‑1 (MQ=60)

                             GTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCG                                                                 <  SRR4302540.3744270/94‑1 (MQ=60)

                             GTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCG                                                                 <  SRR4302540.7946822/94‑1 (MQ=60)

                             GTCAGCGGGTCGATAATGCGTGCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCG                                                                 <  SRR4302540.1962912/94‑1 (MQ=60)

                              TCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGC                                                                >  SRR4302540.6681290/1‑94 (MQ=60)

                               CAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCC                                                               >  SRR4302540.8154836/1‑94 (MQ=60)

                                AGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCA                                                              >  SRR4302540.8463611/1‑94 (MQ=60)

                                AGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCA                                                              >  SRR4302540.3783619/1‑94 (MQ=60)

                                 GCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAG                                                             <  SRR4302540.8229046/94‑1 (MQ=60)

                                 GCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAG                                                             <  SRR4302540.4202911/94‑1 (MQ=60)

                                 GCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGATTTCCCGCCAG                                                             >  SRR4302540.2642779/1‑94 (MQ=60)

                                  CGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGA                                                            >  SRR4302540.2559189/1‑94 (MQ=60)

                                   GGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGAT                                                           <  SRR4302540.51130/94‑1 (MQ=60)

                                   GGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGAT                                                           <  SRR4302540.8877897/94‑1 (MQ=60)

                                   GGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGAT                                                           <  SRR4302540.8708326/94‑1 (MQ=60)

                                    GGTTGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATC                                                          >  SRR4302540.23580/1‑94 (MQ=60)

                                    GGTCGATAATGCGTTCTATCAGGCTTTTGTCGGGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATC                                                          >  SRR4302540.4122134/1‑94 (MQ=60)

                                    GGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATC                                                          <  SRR4302540.5352513/94‑1 (MQ=60)

                                    GGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATC                                                          >  SRR4302540.6848688/1‑94 (MQ=60)

                                     GTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCA                                                         <  SRR4302540.2985013/94‑1 (MQ=60)

                                      gcgataagtCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCAC                                                        <  SRR4302540.2134766/85‑1 (MQ=60)

                                      TCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCAC                                                        <  SRR4302540.667284/94‑1 (MQ=60)

                                       CGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACG                                                       >  SRR4302540.2647619/1‑94 (MQ=60)

                                       CGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACG                                                       <  SRR4302540.6899511/94‑1 (MQ=60)

                                       CGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACG                                                       >  SRR4302540.5113677/1‑94 (MQ=60)

                                        GATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGC                                                      >  SRR4302540.1220916/1‑94 (MQ=60)

                                         ATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCC                                                     >  SRR4302540.4699390/1‑94 (MQ=60)

                                         ATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCA                                                     >  SRR4302540.895605/1‑94 (MQ=60)

                                          TAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCAC                                                    >  SRR4302540.7502281/1‑94 (MQ=60)

                                          TAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCAC                                                    <  SRR4302540.7152989/94‑1 (MQ=60)

                                          TAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCAC                                                    >  SRR4302540.4281368/1‑94 (MQ=60)

                                          TAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCAC                                                    >  SRR4302540.7055941/1‑94 (MQ=60)

                                           AATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGACCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGCTCACGCACC                                                   >  SRR4302540.2247116/1‑94 (MQ=60)

                                              TCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGC                                                <  SRR4302540.2387736/94‑1 (MQ=60)

                                              GCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGC                                                <  SRR4302540.9236116/94‑1 (MQ=60)

                                              GCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGC                                                <  SRR4302540.5580784/94‑1 (MQ=60)

                                                GTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCAACAGCCG                                              >  SRR4302540.3907942/1‑94 (MQ=60)

                                                  TCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCGGCATCAGTTGTACCTGCTTGCCGAGGTTTCGCGCCAGATCACGAAACAGGCGGG                                            >  SRR4302540.1113681/1‑94 (MQ=60)

                                                  TCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGG                                            <  SRR4302540.7475509/94‑1 (MQ=60)

                                                  TCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGG                                            <  SRR4302540.9814652/94‑1 (MQ=60)

                                                   CTATCAGGCTTTGGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGG                                           <  SRR4302540.7542063/94‑1 (MQ=60)

                                                    TATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGA                                          >  SRR4302540.2440976/1‑94 (MQ=60)

                                                    TATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGA                                          >  SRR4302540.6383832/1‑94 (MQ=60)

                                                    TATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGA                                          <  SRR4302540.5608168/94‑1 (MQ=60)

                                                    TATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGA                                          <  SRR4302540.4552462/94‑1 (MQ=60)

                                                     ATCAGGCTTTTGTCGAGTTCAGTCGAACTGCCCACAAGCATAAGTTCTAACTGCTGGCCGGGTGTTCCCGCCAGAgccggcacccgccggggag                                         >  SRR4302540.8394492/1‑75 (MQ=30)

                                                     ATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGAT                                         >  SRR4302540.4358131/1‑94 (MQ=60)

                                                     ATCAGGCTTTTGGCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGAT                                         <  SRR4302540.7173819/94‑1 (MQ=60)

                                                       CAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAG                                       >  SRR4302540.9153848/1‑94 (MQ=60)

                                                       CAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAG                                       >  SRR4302540.8888120/1‑94 (MQ=60)

                                                       CAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAG                                       >  SRR4302540.4830440/1‑94 (MQ=60)

                                                       CAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGCGATAG                                       >  SRR4302540.8887909/1‑94 (MQ=60)

                                                         GGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCG                                     <  SRR4302540.9994855/94‑1 (MQ=60)

                                                          GCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGA                                    <  SRR4302540.6711414/94‑1 (MQ=60)

                                                          GCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGA                                    <  SRR4302540.667863/94‑1 (MQ=60)

                                                          GCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGA                                    >  SRR4302540.8384109/1‑94 (MQ=60)

                                                           CTTTTGTCGAGTTCATCAGAACTGCCCACAAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGAC                                   <  SRR4302540.3387447/94‑1 (MQ=60)

                                                           CTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGAC                                   >  SRR4302540.9640892/1‑94 (MQ=60)

                                                            TTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACT                                  >  SRR4302540.8422521/1‑94 (MQ=60)

                                                             TTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTA                                 >  SRR4302540.8122486/1‑94 (MQ=60)

                                                             TTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTA                                 <  SRR4302540.9254027/94‑1 (MQ=60)

                                                             TTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTA                                 >  SRR4302540.3281624/1‑94 (MQ=60)

                                                              TTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAA                                >  SRR4302540.8336170/1‑94 (MQ=60)

                                                              TTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAA                                >  SRR4302540.902739/1‑94 (MQ=60)

                                                                GTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAA                              >  SRR4302540.3738840/1‑94 (MQ=60)

                                                                GTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAA                              <  SRR4302540.7950185/94‑1 (MQ=60)

                                                                 TCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAA                             <  SRR4302540.6565491/94‑1 (MQ=60)

                                                                 TCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAA                             <  SRR4302540.4548595/94‑1 (MQ=60)

                                                                     GTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATA                         <  SRR4302540.7693757/94‑1 (MQ=60)

                                                                      TTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATAT                        <  SRR4302540.7300538/94‑1 (MQ=60)

                                                                      TTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATAT                        <  SRR4302540.2603633/94‑1 (MQ=60)

                                                                      TTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATAT                        <  SRR4302540.2630919/94‑1 (MQ=60)

                                                                       TCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATT                       <  SRR4302540.8243234/94‑1 (MQ=60)

                                                                         CGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCC                     <  SRR4302540.98167/94‑1 (MQ=60)

                                                                         AGTATAAGTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCC                     <  SRR4302540.6588934/94‑1 (MQ=60)

                                                                         AGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCC                     <  SRR4302540.1429959/94‑1 (MQ=60)

                                                                           TAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCAT                   <  SRR4302540.5764412/94‑1 (MQ=60)

                                                                            AGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATC                  <  SRR4302540.2959093/94‑1 (MQ=60)

                                                                            AGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATC                  <  SRR4302540.2959094/94‑1 (MQ=60)

                                                                            AGAACTCCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATC                  <  SRR4302540.7297676/94‑1 (MQ=60)

                                                                             GAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCG                 <  SRR4302540.3268307/94‑1 (MQ=60)

                                                                             GAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCG                 <  SRR4302540.761182/94‑1 (MQ=60)

                                                                              AACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGG                >  SRR4302540.9290426/1‑94 (MQ=60)

                                                                               ACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGC               >  SRR4302540.5565732/1‑94 (MQ=60)

                                                                                 TGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCAT             <  SRR4302540.8302353/94‑1 (MQ=60)

                                                                                 TGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCAT             <  SRR4302540.7123978/94‑1 (MQ=60)

                                                                                   CCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCA           <  SRR4302540.3516954/94‑1 (MQ=60)

                                                                                   CCCACAAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCA           <  SRR4302540.4376194/94‑1 (MQ=60)

                                                                                    CCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATACCATCGGCCTCAT          >  SRR4302540.16728/1‑94 (MQ=60)

                                                                                     CACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATG         <  SRR4302540.78/94‑1 (MQ=60)

                                                                                     CACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATG         <  SRR4302540.1306146/94‑1 (MQ=60)

                                                                                      ACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATGC        <  SRR4302540.1809729/94‑1 (MQ=60)

                                                                                       CCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATGCG       <  SRR4302540.8415545/94‑1 (MQ=60)

                                                                                        CAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATGCGA      >  SRR4302540.354496/1‑94 (MQ=60)

                                                                                         AGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATGCGAA     >  SRR4302540.3650341/1‑94 (MQ=60)

                                                                                         AGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATGCGAA     >  SRR4302540.2569481/1‑94 (MQ=60)

                                                                                         AGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATGCGAA     >  SRR4302540.4441155/1‑94 (MQ=60)

                                                                                          GCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATGCGAAT    <  SRR4302540.2937894/94‑1 (MQ=60)

                                                                                          GCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATGCGAAT    >  SRR4302540.4762910/1‑94 (MQ=60)

                                                                                           CATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATGCGAATC   >  SRR4302540.3935023/1‑94 (MQ=60)

                                                                                           CATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATGCGAATC   <  SRR4302540.8228174/94‑1 (MQ=60)

                                                                                           CATCAGTTCTACCTGCTTGCCGAGTATTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATGCGAATC   <  SRR4302540.6817278/94‑1 (MQ=60)

                                                                                            ATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCCTGCGAATCG  >  SRR4302540.4688094/1‑94 (MQ=60)

                                                                                            ATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATGCGAATCG  >  SRR4302540.3218972/1‑94 (MQ=60)

                                                                                            ATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATGCGAATCG  >  SRR4302540.4688095/1‑94 (MQ=60)

                                                                                            ATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATGCGAATCA  >  SRR4302540.5213156/1‑94 (MQ=60)

CGTGATCGAGGCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCGTCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATGCGAATCG  >  NC_000913/1972196‑1972381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`