Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,977,687 T→C intergenic (‑448/‑90) insA5 ← / → yecG IS1 protein InsA/putative regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,977,6870TC94.8% 1143.4 / 34.7 387intergenic (‑448/‑90)insA5/yecGIS1 protein InsA/putative regulator
Reads supporting (aligned to +/- strand):  ref base T (10/10);  new base C (203/164);  total (213/174)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.75e-01

Only 100 of 394 total aligned reads displayed.

CAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTTACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGTT  >  NC_000913/1977596‑1977776
                                                                                           |                                                                                         
cAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTTa                                                                                          <  2:7731711/93‑1 (MQ=255)
  gTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTTAcc                                                                                        <  2:3644101/93‑1 (MQ=255)
   tACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCt                                                                                       >  1:8057063/1‑93 (MQ=255)
     cacTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGt                                                                                     >  2:8900547/1‑93 (MQ=255)
     cacTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGt                                                                                     <  2:4957511/93‑1 (MQ=255)
      acTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGtt                                                                                    <  1:1433691/93‑1 (MQ=255)
      acTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGtt                                                                                    >  1:8495080/1‑93 (MQ=255)
      acTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGtt                                                                                    <  2:2784569/93‑1 (MQ=255)
       cTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGttt                                                                                   >  1:1218188/1‑93 (MQ=255)
       cTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGttt                                                                                   >  1:3866420/1‑93 (MQ=255)
          ttttCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGAc                                                                                >  2:672956/1‑93 (MQ=255)
            ttCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCg                                                                              <  1:975099/93‑1 (MQ=255)
             tCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGa                                                                             >  1:7196132/1‑93 (MQ=255)
                tataATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGtt                                                                          <  1:9656802/93‑1 (MQ=255)
                tataATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGtt                                                                          <  2:482124/93‑1 (MQ=255)
                 ataATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTg                                                                         <  2:2303312/93‑1 (MQ=255)
                 ataATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTg                                                                         >  2:3474162/1‑93 (MQ=255)
                  taATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTgg                                                                        <  2:5384923/93‑1 (MQ=255)
                  taATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTgg                                                                        >  1:1830400/1‑93 (MQ=255)
                    aTTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGa                                                                      <  2:656432/93‑1 (MQ=255)
                    aTTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGa                                                                      >  1:8174229/1‑93 (MQ=255)
                    aTTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGa                                                                      >  2:8263725/1‑93 (MQ=255)
                     ttACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGAt                                                                     >  1:8448919/1‑93 (MQ=255)
                      tACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGAtt                                                                    <  2:7146709/93‑1 (MQ=255)
                          tttGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGcgt                                                                <  1:6744471/93‑1 (MQ=255)
                               aaTTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGttt                                                           <  2:4167128/93‑1 (MQ=255)
                               aaTTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGttt                                                           <  1:9085574/93‑1 (MQ=255)
                               aaTTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGttt                                                           <  1:9526289/93‑1 (MQ=255)
                               aaTTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGttt                                                           <  1:3501689/93‑1 (MQ=255)
                                aTTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTtc                                                          <  2:3770455/93‑1 (MQ=255)
                                 ttATATGATTACCGGGAGTGATTGATTAATTATCTTTACTAATAATCCGACTAATATTTACCTGTTTGACCGAGATGGGAATGCGACGTTtct                                                         >  2:1813883/1‑93 (MQ=255)
                                 ttATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTTACCTGTTTGACCGAGTTGGGATTGCGTCGTTtct                                                         >  1:4628164/1‑93 (MQ=255)
                                 ttATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTtct                                                         >  1:293123/1‑93 (MQ=255)
                                   atatGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTcc                                                       >  1:1971214/1‑93 (MQ=255)
                                    tatGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCa                                                      <  1:9212439/93‑1 (MQ=255)
                                    tatGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCa                                                      <  1:26545/93‑1 (MQ=255)
                                     atGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGATTGACCGAGTTGGGATTGCGGCGTTTCTCCAt                                                     >  1:4936496/1‑93 (MQ=255)
                                      ttaTCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCAtt                                                    <  1:3374750/91‑1 (MQ=255)
                                      tGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCAtt                                                    >  1:5599285/1‑93 (MQ=255)
                                       gATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTa                                                   <  1:7708471/93‑1 (MQ=255)
                                       gATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTa                                                   <  2:7912243/93‑1 (MQ=255)
                                       gATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTa                                                   <  1:5996771/93‑1 (MQ=255)
                                        aTCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAg                                                  <  2:430244/93‑1 (MQ=255)
                                         tCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAgg                                                 <  1:2172550/93‑1 (MQ=255)
                                          cACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGa                                                >  2:3961444/1‑93 (MQ=255)
                                          cACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGa                                                <  2:1399255/93‑1 (MQ=255)
                                            ccGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGt                                              <  2:7080250/93‑1 (MQ=255)
                                               ggAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTaaa                                           >  2:4095374/1‑93 (MQ=255)
                                               ggAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTaaa                                           >  2:696837/1‑93 (MQ=255)
                                                gAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTACAg                                          >  1:4343914/1‑93 (MQ=255)
                                                 aGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGc                                         <  2:9023251/93‑1 (MQ=255)
                                                  gTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCt                                        <  2:9193324/93‑1 (MQ=255)
                                                   tgattgatTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCtt                                       >  2:6837594/1‑93 (MQ=255)
                                                    gattgattAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCttt                                      >  2:6337693/1‑93 (MQ=255)
                                                     attgattAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTa                                     <  1:4104499/93‑1 (MQ=255)
                                                      ttgattAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTaa                                    >  1:5154440/1‑93 (MQ=255)
                                                      ttgattAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTaa                                    <  2:5888324/93‑1 (MQ=255)
                                                      ttgattAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTaa                                    <  1:7050866/93‑1 (MQ=255)
                                                       tgattAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAAt                                   >  1:231128/1‑93 (MQ=255)
                                                        gattAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATg                                  >  2:455429/1‑93 (MQ=255)
                                                        gattAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATg                                  <  2:8491213/93‑1 (MQ=255)
                                                        gattAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATg                                  >  2:316452/1‑93 (MQ=255)
                                                        gattAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATg                                  >  1:6188784/1‑93 (MQ=255)
                                                         attaattaTCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGt                                 <  2:1718586/93‑1 (MQ=255)
                                                          ttaattaTCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTc                                <  2:9796104/93‑1 (MQ=255)
                                                           taattaTCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCa                               >  1:6404529/1‑93 (MQ=255)
                                                           taattaTCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCa                               >  1:8694681/1‑93 (MQ=255)
                                                             attaTCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCAcc                             >  1:1044647/1‑93 (MQ=255)
                                                             attaTCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCAcc                             >  1:4227715/1‑93 (MQ=255)
                                                              ttaTCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCt                            >  1:1544469/1‑93 (MQ=255)
                                                               taTCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTg                           <  2:9452156/93‑1 (MQ=255)
                                                                ttCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGa                          <  1:8357990/92‑1 (MQ=255)
                                                                 tCTTTACTAATAATCAGACTAATATTTACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGaa                         <  2:9667457/93‑1 (MQ=255)
                                                                 tCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGaa                         >  1:5339962/1‑93 (MQ=255)
                                                                 tCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGaa                         >  2:2499076/1‑93 (MQ=255)
                                                                  cTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAg                        <  2:5132314/93‑1 (MQ=255)
                                                                  cTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAg                        >  1:8856161/1‑93 (MQ=255)
                                                                      aCTAATAATCAGACTAATATTTACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTca                    >  1:581297/1‑93 (MQ=255)
                                                                      aCTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTca                    >  1:9960906/1‑93 (MQ=255)
                                                                       cTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTcac                   >  2:2086249/1‑93 (MQ=255)
                                                                       cTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTcac                   <  2:8746969/93‑1 (MQ=255)
                                                                       cTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTcac                   <  2:7985696/93‑1 (MQ=255)
                                                                          ataatCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGa                >  2:7382907/1‑93 (MQ=255)
                                                                           taatCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGaa               <  2:530390/93‑1 (MQ=255)
                                                                           taatCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGaa               >  2:3122795/1‑93 (MQ=255)
                                                                             atCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATa             <  2:1059335/93‑1 (MQ=255)
                                                                             atCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATa             <  2:293635/93‑1 (MQ=255)
                                                                             atCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATa             >  1:3339231/1‑93 (MQ=255)
                                                                              tCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATaa            >  1:7487598/1‑93 (MQ=255)
                                                                              tCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATaa            >  2:6222914/1‑93 (MQ=255)
                                                                                aGACTAATATTTACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAgg          >  2:5592655/1‑93 (MQ=255)
                                                                                aGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAgg          <  1:7903237/93‑1 (MQ=255)
                                                                                aGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAgg          <  2:1137616/93‑1 (MQ=255)
                                                                                aGACTAATATTCACCTGTTTGACCGAGGTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCCCCTGAAGTTCACAGAATAAga          >  1:2539321/1‑92 (MQ=255)
                                                                                 gACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGa         >  2:8130502/1‑93 (MQ=255)
                                                                                  aCTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGaa        <  1:7022086/93‑1 (MQ=255)
                                                                                     aaTATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAg     <  2:3003986/93‑1 (MQ=255)
                                                                                      atatTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAgg    >  2:1573129/1‑93 (MQ=255)
                                                                                      atatTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAgg    <  2:6386434/93‑1 (MQ=255)
                                                                                       tatTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGt   >  1:1720772/1‑93 (MQ=255)
                                                                                           |                                                                                         
CAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTTACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGTT  >  NC_000913/1977596‑1977776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 204 total aligned reads displayed.
ATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTTACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGTTATGA  >  NC_000913/1977594‑1977780
                                                                                             |                                                                                             
ATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTC                                                                                               <  SRR4302540.3273378/94‑1 (MQ=60)
ATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTC                                                                                               >  SRR4302540.3023153/1‑94 (MQ=60)
   AGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACC                                                                                            >  SRR4302540.1514669/1‑94 (MQ=60)
   AGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACC                                                                                            <  SRR4302540.5103531/94‑1 (MQ=60)
    GTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTTACCT                                                                                           <  SRR4302540.9260784/94‑1 (MQ=60)
    GTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCT                                                                                           >  SRR4302540.8150618/1‑94 (MQ=60)
    GTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCT                                                                                           >  SRR4302540.8150617/1‑94 (MQ=60)
    GTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCT                                                                                           >  SRR4302540.3695523/1‑94 (MQ=60)
    GTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCT                                                                                           <  SRR4302540.7109538/94‑1 (MQ=60)
    GTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCT                                                                                           <  SRR4302540.9664043/94‑1 (MQ=60)
     TACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTG                                                                                          >  SRR4302540.585398/1‑94 (MQ=60)
     TACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTG                                                                                          >  SRR4302540.7195833/1‑94 (MQ=60)
       CACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTT                                                                                        >  SRR4302540.8593793/1‑94 (MQ=60)
       CACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTT                                                                                        <  SRR4302540.3389876/94‑1 (MQ=60)
        ACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTT                                                                                       <  SRR4302540.1449234/94‑1 (MQ=60)
        ACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTT                                                                                       >  SRR4302540.3908449/1‑94 (MQ=60)
        ACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTT                                                                                       >  SRR4302540.1230658/1‑94 (MQ=60)
        ACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTT                                                                                       >  SRR4302540.1230659/1‑94 (MQ=60)
        ACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTT                                                                                       <  SRR4302540.8137246/94‑1 (MQ=60)
         CTATTTTCTTATAATTACATTTGAAATTATATGATCACCAGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTG                                                                                      >  SRR4302540.9091363/1‑94 (MQ=60)
          TATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGA                                                                                     >  SRR4302540.6831187/1‑94 (MQ=60)
           ATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGAC                                                                                    >  SRR4302540.2327759/1‑94 (MQ=60)
            TTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACC                                                                                   <  SRR4302540.4074355/94‑1 (MQ=60)
            TTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACC                                                                                   <  SRR4302540.4331979/94‑1 (MQ=60)
            TTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACC                                                                                   <  SRR4302540.1919102/94‑1 (MQ=60)
             TTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCG                                                                                  <  SRR4302540.3269775/94‑1 (MQ=60)
             TTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCG                                                                                  >  SRR4302540.8514808/1‑94 (MQ=60)
             TTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCG                                                                                  >  SRR4302540.1649279/1‑94 (MQ=60)
              TTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGA                                                                                 >  SRR4302540.8896387/1‑94 (MQ=60)
              TTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGA                                                                                 <  SRR4302540.985109/94‑1 (MQ=60)
              TTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGA                                                                                 >  SRR4302540.7276345/1‑94 (MQ=60)
                CTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGT                                                                               >  SRR4302540.4022367/1‑94 (MQ=60)
                 TTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTT                                                                              <  SRR4302540.4276861/94‑1 (MQ=60)
                 TTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTT                                                                              <  SRR4302540.7416311/94‑1 (MQ=60)
                  TATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTCATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTG                                                                             >  SRR4302540.5245285/1‑94 (MQ=60)
                  TATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTG                                                                             <  SRR4302540.9770480/94‑1 (MQ=60)
                  TATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTG                                                                             >  SRR4302540.9766209/1‑94 (MQ=60)
                  TATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTG                                                                             >  SRR4302540.6389776/1‑94 (MQ=60)
                   ATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGG                                                                            <  SRR4302540.2229513/94‑1 (MQ=60)
                   ATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGG                                                                            >  SRR4302540.1820555/1‑94 (MQ=60)
                   ATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGG                                                                            >  SRR4302540.1850426/1‑94 (MQ=60)
                    TAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGG                                                                           <  SRR4302540.2816533/94‑1 (MQ=60)
                    TAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGG                                                                           >  SRR4302540.9276517/1‑94 (MQ=60)
                     AATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGA                                                                          >  SRR4302540.8269179/1‑94 (MQ=60)
                     AATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGA                                                                          >  SRR4302540.3207685/1‑94 (MQ=60)
                      ATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGAT                                                                         >  SRR4302540.494225/1‑94 (MQ=60)
                      ATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGAT                                                                         >  SRR4302540.8547006/1‑94 (MQ=60)
                      ATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGAT                                                                         <  SRR4302540.21697/94‑1 (MQ=60)
                      ATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGAT                                                                         >  SRR4302540.8547005/1‑94 (MQ=60)
                      ATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGAT                                                                         <  SRR4302540.9295585/94‑1 (MQ=60)
                       TTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATT                                                                        >  SRR4302540.2573147/1‑94 (MQ=60)
                       TTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATT                                                                        <  SRR4302540.18998/94‑1 (MQ=60)
                       TTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATT                                                                        >  SRR4302540.9795965/1‑94 (MQ=60)
                       TTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATT                                                                        >  SRR4302540.7498555/1‑94 (MQ=60)
                         ACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGC                                                                      <  SRR4302540.1984129/94‑1 (MQ=60)
                           ATTT‑AAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTC                                                                   <  SRR4302540.1611724/94‑1 (MQ=60)
                            TTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTTACCTGTTTGACCGAGTTGGGATTGCGTC                                                                   <  SRR4302540.5368973/94‑1 (MQ=60)
                            TTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTC                                                                   >  SRR4302540.8896341/1‑94 (MQ=60)
                            TTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTC                                                                   <  SRR4302540.3475791/94‑1 (MQ=60)
                            TTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTC                                                                   <  SRR4302540.6818992/94‑1 (MQ=60)
                             TTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCG                                                                  <  SRR4302540.7589224/94‑1 (MQ=60)
                             TTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCG                                                                  >  SRR4302540.1792278/1‑94 (MQ=60)
                              TGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGT                                                                 >  SRR4302540.578645/1‑94 (MQ=60)
                              TGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGT                                                                 >  SRR4302540.1506126/1‑94 (MQ=60)
                                AAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGTATTGCGTCGTTT                                                               <  SRR4302540.6301985/94‑1 (MQ=60)
                                AAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTT                                                               >  SRR4302540.5433964/1‑94 (MQ=60)
                                AAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTT                                                               <  SRR4302540.5484706/94‑1 (MQ=60)
                                 AATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTC                                                              <  SRR4302540.8061957/94‑1 (MQ=60)
                                 AATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTC                                                              <  SRR4302540.3539828/94‑1 (MQ=60)
                                 AATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTC                                                              <  SRR4302540.9194729/94‑1 (MQ=60)
                                 AATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTC                                                              <  SRR4302540.9639081/94‑1 (MQ=60)
                                  ATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTTACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCT                                                             >  SRR4302540.4677745/1‑94 (MQ=60)
                                  ATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCT                                                             >  SRR4302540.2006588/1‑94 (MQ=60)
                                  ATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCT                                                             >  SRR4302540.296020/1‑94 (MQ=60)
                                    TATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCC                                                           >  SRR4302540.1992615/1‑94 (MQ=60)
                                    TATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCC                                                           <  SRR4302540.1554261/94‑1 (MQ=60)
                                     ATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCA                                                          <  SRR4302540.9912880/94‑1 (MQ=60)
                                      TATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCAT                                                         <  SRR4302540.8363706/94‑1 (MQ=60)
                                      TATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCAT                                                         <  SRR4302540.7935704/94‑1 (MQ=60)
                                      TATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCAT                                                         <  SRR4302540.26832/94‑1 (MQ=60)
                                      TATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCAT                                                         >  SRR4302540.6999776/1‑94 (MQ=60)
                                      TATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCAT                                                         <  SRR4302540.9322973/94‑1 (MQ=60)
                                      TATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGATTGACCGAGTTGGGATTGCGGCGTTTCTCCAT                                                         >  SRR4302540.4989748/1‑94 (MQ=60)
                                       ATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATT                                                        >  SRR4302540.5660886/1‑94 (MQ=60)
                                       ATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATT                                                        >  SRR4302540.1361341/1‑94 (MQ=60)
                                        TTATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTA                                                       <  SRR4302540.3411630/94‑1 (MQ=60)
                                        TGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTA                                                       <  SRR4302540.711993/94‑1 (MQ=60)
                                        TGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTA                                                       >  SRR4302540.8455515/1‑94 (MQ=60)
                                         GATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAG                                                      <  SRR4302540.5384794/94‑1 (MQ=60)
                                         GATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAG                                                      <  SRR4302540.7796938/94‑1 (MQ=60)
                                         GATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAG                                                      <  SRR4302540.6063223/94‑1 (MQ=60)
                                         GATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAG                                                      >  SRR4302540.9570566/1‑94 (MQ=60)
                                          ATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGG                                                     >  SRR4302540.9469066/1‑94 (MQ=60)
                                          ATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGG                                                     <  SRR4302540.2337249/94‑1 (MQ=60)
                                           TCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGCGTTGGGATTGCGTCGTTTCTCCATTAGGA                                                    >  SRR4302540.5997744/1‑94 (MQ=60)
                                           TCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGA                                                    <  SRR4302540.2195978/94‑1 (MQ=60)
                                            CACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAG                                                   <  SRR4302540.9157724/94‑1 (MQ=60)
                                             ACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGT                                                  <  SRR4302540.5206814/94‑1 (MQ=60)
                                             ACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGT                                                  >  SRR4302540.370804/1‑94 (MQ=60)
                                              CCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTA                                                 >  SRR4302540.7446696/1‑94 (MQ=60)
                                                GGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAA                                               <  SRR4302540.6069582/94‑1 (MQ=60)
                                                GGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAA                                               >  SRR4302540.2628883/1‑94 (MQ=60)
                                                 GGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTACAG                                              >  SRR4302540.4391197/1‑94 (MQ=60)
                                                 GGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAG                                              <  SRR4302540.4390869/94‑1 (MQ=60)
                                                 GGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAG                                              <  SRR4302540.5068972/94‑1 (MQ=60)
                                                  GAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGC                                             <  SRR4302540.9628271/94‑1 (MQ=60)
                                                     TGATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTT                                          <  SRR4302540.6162077/94‑1 (MQ=60)
                                                       ATTGATTAATTATCTTTACTAATAATCATACTAATATTTTCCTGTTTGACCGAGTTGGGATTGCGTCGTTTCGCCATTAGGAGTAAAGCTTTAA                                        <  SRR4302540.7161417/94‑1 (MQ=60)
                                                       ATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAA                                        >  SRR4302540.5210380/1‑94 (MQ=60)
                                                       ATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAA                                        <  SRR4302540.4148776/94‑1 (MQ=60)
                                                       ATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAA                                        >  SRR4302540.8773469/1‑94 (MQ=60)
                                                       ATTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAA                                        >  SRR4302540.7771937/1‑94 (MQ=60)
                                                        TTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAAT                                       <  SRR4302540.7129280/94‑1 (MQ=60)
                                                        TTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAAT                                       <  SRR4302540.7129281/94‑1 (MQ=60)
                                                        TTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAAT                                       >  SRR4302540.5998131/1‑94 (MQ=60)
                                                        TTGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAAT                                       >  SRR4302540.233363/1‑94 (MQ=60)
                                                         TGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATG                                      <  SRR4302540.8577033/94‑1 (MQ=60)
                                                         TGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATG                                      >  SRR4302540.221896/1‑94 (MQ=60)
                                                         TGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATG                                      <  SRR4302540.8577034/94‑1 (MQ=60)
                                                         TGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATG                                      >  SRR4302540.6257692/1‑94 (MQ=60)
                                                         TGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATG                                      >  SRR4302540.3800738/1‑94 (MQ=60)
                                                         TGATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATG                                      <  SRR4302540.2838431/94‑1 (MQ=60)
                                                         TGATTAATTATCTTTACTAATAATCAGACTAATATTCACATGTTTGCCCGAGTTGGGATTGCGTCGTTTCTCCtttacgtctacatgtttaatg                                      >  SRR4302540.4348641/1‑73 (MQ=60)
                                                          GATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGT                                     <  SRR4302540.4148110/94‑1 (MQ=60)
                                                          GATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGT                                     >  SRR4302540.1226268/1‑94 (MQ=60)
                                                          GATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGT                                     <  SRR4302540.9700215/94‑1 (MQ=60)
                                                          GATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGT                                     >  SRR4302540.6423786/1‑94 (MQ=60)
                                                           ATTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTC                                    <  SRR4302540.2826015/94‑1 (MQ=60)
                                                           ATTAATTATCTTTACTAATAATCAGACTAACATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTC                                    >  SRR4302540.1790341/1‑94 (MQ=60)
                                                            TTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCA                                   >  SRR4302540.8795619/1‑94 (MQ=60)
                                                            TTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCA                                   >  SRR4302540.6475819/1‑94 (MQ=60)
                                                            TTAATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCA                                   >  SRR4302540.6475818/1‑94 (MQ=60)
                                                              AATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACC                                 >  SRR4302540.4273741/1‑94 (MQ=60)
                                                              AATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACC                                 >  SRR4302540.1055263/1‑94 (MQ=60)
                                                               ATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTGGTTTCTCCATTCGGAGTAAAGCTTTAATGTCACCT                                >  SRR4302540.3488425/1‑94 (MQ=60)
                                                               ATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCT                                >  SRR4302540.5866697/1‑94 (MQ=60)
                                                               ATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCT                                >  SRR4302540.4533248/1‑94 (MQ=60)
                                                               ATTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCT                                >  SRR4302540.1561521/1‑94 (MQ=60)
                                                                TTATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTG                               <  SRR4302540.8455612/94‑1 (MQ=60)
                                                                 TATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGA                              >  SRR4302540.1563316/1‑94 (MQ=60)
                                                                  TTCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAA                             <  SRR4302540.8454978/94‑1 (MQ=60)
                                                                  ATCTTTACTAATAATGAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAA                             <  SRR4302540.8454976/94‑1 (MQ=60)
                                                                  ATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAA                             <  SRR4302540.8454977/94‑1 (MQ=60)
                                                                  ATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAA                             >  SRR4302540.5398511/1‑94 (MQ=60)
                                                                  ATCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAA                             <  SRR4302540.7843122/94‑1 (MQ=60)
                                                                   TCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAG                            <  SRR4302540.658760/94‑1 (MQ=60)
                                                                   TCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAG                            >  SRR4302540.8958628/1‑94 (MQ=60)
                                                                   TCTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAG                            >  SRR4302540.1433845/1‑94 (MQ=60)
                                                                    CTTTACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGT                           <  SRR4302540.5525864/94‑1 (MQ=60)
                                                                       TACTAATAATCAGACTAATATTTACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCA                        >  SRR4302540.587581/1‑94 (MQ=60)
                                                                       TACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCA                        >  SRR4302540.8662662/1‑94 (MQ=60)
                                                                       TACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCA                        >  SRR4302540.7164160/1‑94 (MQ=60)
                                                                       TACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCA                        >  SRR4302540.10077137/1‑94 (MQ=60)
                                                                       TACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTAA                        >  SRR4302540.3177765/1‑94 (MQ=60)
                                                                        ACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCAC                       <  SRR4302540.4170769/94‑1 (MQ=60)
                                                                        ACTAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCAC                       >  SRR4302540.4540504/1‑94 (MQ=60)
                                                                          TAATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAG                     >  SRR4302540.9932790/1‑94 (MQ=60)
                                                                            ATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAA                   <  SRR4302540.7079492/94‑1 (MQ=60)
                                                                            ATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAA                   <  SRR4302540.3442815/94‑1 (MQ=60)
                                                                            ATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAA                   >  SRR4302540.3401101/1‑94 (MQ=60)
                                                                            ATAATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAA                   >  SRR4302540.3396857/1‑94 (MQ=60)
                                                                            ATAATCAGACTAATATTCACCTGTTTGACCGAGATGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAA                   <  SRR4302540.9802312/94‑1 (MQ=60)
                                                                              AATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATA                 >  SRR4302540.3375890/1‑94 (MQ=60)
                                                                              AATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATA                 <  SRR4302540.88886/94‑1 (MQ=60)
                                                                               ATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAA                <  SRR4302540.8184484/94‑1 (MQ=60)
                                                                               ATCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAA                >  SRR4302540.7571180/1‑94 (MQ=60)
                                                                                TCAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAG               >  SRR4302540.6764286/1‑94 (MQ=60)
                                                                                 CAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGG              >  SRR4302540.2566702/1‑94 (MQ=60)
                                                                                 CAGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGG              >  SRR4302540.2543743/1‑94 (MQ=60)
                                                                                 CAGACTAATATTCACCTGTTTGACCGAGGTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCCCCTGAAGTTCACAGAATAAGA              >  SRR4302540.2566701/1‑94 (MQ=60)
                                                                                  AGACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGA             <  SRR4302540.7995408/94‑1 (MQ=60)
                                                                                    ACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAAC           >  SRR4302540.4941540/1‑94 (MQ=60)
                                                                                    ACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAAC           <  SRR4302540.7100088/94‑1 (MQ=60)
                                                                                    ACTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAAC           >  SRR4302540.9013075/1‑94 (MQ=60)
                                                                                    ACTAATATTCACCTGTTTGACCGAGATGGGATTGCGTCGTTACTCCATTAGGAGTAAAGCTTTAATGTCCCCTGAAGTTCACGGAATAAGGAAC           <  SRR4302540.7100089/94‑1 (MQ=60)
                                                                                     CTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACA          >  SRR4302540.492880/1‑94 (MQ=60)
                                                                                     CTAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACA          <  SRR4302540.5887416/94‑1 (MQ=60)
                                                                                      TAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACCG         >  SRR4302540.7786136/1‑94 (MQ=60)
                                                                                      TAATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAG         <  SRR4302540.2531086/94‑1 (MQ=60)
                                                                                       AATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGG        >  SRR4302540.1071624/1‑94 (MQ=60)
                                                                                        ATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGT       <  SRR4302540.4634714/94‑1 (MQ=60)
                                                                                        ATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGT       >  SRR4302540.8636252/1‑94 (MQ=60)
                                                                                        ATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGT       >  SRR4302540.1739347/1‑94 (MQ=60)
                                                                                        ATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGT       >  SRR4302540.2091697/1‑94 (MQ=60)
                                                                                        ATATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGT       <  SRR4302540.621723/94‑1 (MQ=60)
                                                                                         TATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGTT      >  SRR4302540.9820273/1‑94 (MQ=60)
                                                                                          ATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGCGAAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGTTA     >  SRR4302540.9179978/1‑94 (MQ=60)
                                                                                          ATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGTTA     <  SRR4302540.4238285/94‑1 (MQ=60)
                                                                                          ATTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGTTA     >  SRR4302540.4979346/1‑94 (MQ=60)
                                                                                           TTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGTTAT    <  SRR4302540.8347178/94‑1 (MQ=60)
                                                                                           TTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGTTAT    <  SRR4302540.2205325/94‑1 (MQ=60)
                                                                                           TTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGTTAT    >  SRR4302540.6762522/1‑94 (MQ=60)
                                                                                           TTCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGTTAT    >  SRR4302540.1262713/1‑94 (MQ=60)
                                                                                           TTCACCTGTGTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGTTAT    <  SRR4302540.2205323/94‑1 (MQ=60)
                                                                                            TCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGTTATG   <  SRR4302540.2200220/94‑1 (MQ=60)
                                                                                            TCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGTTATG   <  SRR4302540.281626/94‑1 (MQ=60)
                                                                                            TCACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGTTATG   >  SRR4302540.8091689/1‑94 (MQ=60)
                                                                                             CACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGACTAAGGAACAGGTTATGA  >  SRR4302540.9012644/1‑94 (MQ=60)
                                                                                             CACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGTTATGA  >  SRR4302540.5878683/1‑94 (MQ=60)
                                                                                             CACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGTTATGA  <  SRR4302540.535439/94‑1 (MQ=60)
                                                                                             |                                                                                             
ATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTTACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGTTATGA  >  NC_000913/1977594‑1977780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑