Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,017,469	G→A	T354T (ACG→ACA)	fliK →	flagellar hook‑length control protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,017,469	0	G	A	99.2%	854.6 / ‑5.9	246	T354T (ACG→ACA)	fliK	flagellar hook‑length control protein
Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (136/107); total (137/109)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 260 total aligned reads displayed.

CTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCC  >  NC_000913/2017379‑2017558

cTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACt                                                                                         >  1:6460125/1‑93 (MQ=255)

 tttAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACtt                                                                                        >  2:9891005/1‑93 (MQ=255)

 tttAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACtt                                                                                        <  2:3503801/93‑1 (MQ=255)

  ttAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTc                                                                                       <  2:6650330/93‑1 (MQ=255)

  ttAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTc                                                                                       >  1:1417163/1‑93 (MQ=255)

  ttAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTc                                                                                       >  2:5120299/1‑93 (MQ=255)

  ttAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTc                                                                                       <  2:9568841/93‑1 (MQ=255)

  ttAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTc                                                                                       >  1:2575746/1‑93 (MQ=255)

   tAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTcc                                                                                      <  2:2690386/93‑1 (MQ=255)

    aGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCg                                                                                     <  1:6670783/93‑1 (MQ=255)

     gTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCCAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTGCCgt                                                                                    >  2:5543400/1‑92 (MQ=255)

      tGGTCAGCAGCAGGCCGCTTCCCATCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGt                                                                                   >  1:4478288/1‑93 (MQ=255)

       ggTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGtt                                                                                  >  2:8524459/1‑93 (MQ=255)

         tCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTcc                                                                                >  1:3661710/1‑93 (MQ=255)

            gcagcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGt                                                                             <  2:2750772/93‑1 (MQ=255)

            gcagcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGt                                                                             <  1:9267494/93‑1 (MQ=255)

              agcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGtct                                                                           <  2:7939427/93‑1 (MQ=255)

                cagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAACCCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTctct                                                                         <  1:2281443/93‑1 (MQ=255)

                  ggCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCttt                                                                       <  2:6249181/93‑1 (MQ=255)

                   gCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTa                                                                      <  2:730048/93‑1 (MQ=255)

                     cGCTCCCCTGCAACAGCAAAGTCAACGCACAGCAAACTATGAACCTCTGGCGGGGGTAGACGACGATACACTTCCTGTTCCCGTCTCTCTACa                                                                    >  2:8923813/1‑93 (MQ=255)

                      gCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACaa                                                                   >  2:6102662/1‑93 (MQ=255)

                      gCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACaa                                                                   <  1:5361001/93‑1 (MQ=255)

                      gCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACaa                                                                   >  1:251241/1‑93 (MQ=255)

                       cTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAg                                                                  >  1:2069350/1‑93 (MQ=255)

                        ttCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAgg                                                                 <  2:3081920/93‑1 (MQ=255)

                         tCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAggg                                                                <  2:833978/93‑1 (MQ=255)

                            cagcaacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCgt                                                             <  2:7562146/93‑1 (MQ=255)

                             agcaacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCgtg                                                            >  2:4316849/1‑93 (MQ=255)

                             agcaacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCgtg                                                            <  1:1669578/93‑1 (MQ=255)

                             agcaacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAAGGCgtg                                                            >  1:1393509/1‑93 (MQ=255)

                              gcaacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCgtgt                                                           >  1:4325574/1‑93 (MQ=255)

                               caacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTa                                                          >  2:2249834/1‑93 (MQ=255)

                               caacagcaaAGCAAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTa                                                          <  2:5676499/93‑1 (MQ=255)

                               caacagcaaACCCAAATCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATCCACTTCCGGTTCTCGTCTCTTTACACGGGCGTGTa                                                          >  2:7766303/1‑93 (MQ=255)

                                aacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTaa                                                         <  2:8394150/93‑1 (MQ=255)

                                aacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTaa                                                         <  1:4001727/93‑1 (MQ=255)

                                 acagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAAc                                                        >  1:4219292/1‑93 (MQ=255)

                                  cagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACa                                                       <  1:6911864/93‑1 (MQ=255)

                                  cagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACa                                                       <  1:8597653/93‑1 (MQ=255)

                                    gcaaAGCCACCGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACCAGCGCGTGTAACAgg                                                     >  2:6877108/1‑93 (MQ=255)

                                    gcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAgg                                                     <  2:8112992/93‑1 (MQ=255)

                                    gcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAgg                                                     >  1:4474980/1‑93 (MQ=255)

                                       aaGCCAACGCTCAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCaa                                                  >  2:6553018/1‑93 (MQ=255)

                                        aGCCACCGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAAc                                                 <  2:3885503/93‑1 (MQ=255)

                                        aGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAAc                                                 <  1:5919177/93‑1 (MQ=255)

                                        aGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAAc                                                 <  2:3661710/93‑1 (MQ=255)

                                        aGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCAGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAAc                                                 >  2:5416861/1‑93 (MQ=255)

                                          ccACCGCACAGCAAACCATGACCCTCTGGCGGGGGAAGACGACGATACCCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAg                                               <  2:9062360/93‑1 (MQ=255)

                                          ccAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAg                                               <  1:4532325/93‑1 (MQ=255)

                                           cAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAgc                                              <  1:4340712/93‑1 (MQ=255)

                                           cAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCAGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAgc                                              >  1:4615696/1‑93 (MQ=255)

                                            aaCGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAgcg                                             >  1:9804454/1‑93 (MQ=255)

                                             aCGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAgcgg                                            <  2:3253782/93‑1 (MQ=255)

                                                cacaGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGt                                         >  1:7390558/1‑93 (MQ=255)

                                                  caGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTg                                       >  2:2951878/1‑93 (MQ=255)

                                                   aGCAAGCCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGa                                      <  1:3914316/93‑1 (MQ=255)

                                                    gCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGat                                     <  2:8174076/93‑1 (MQ=255)

                                                    gCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGat                                     >  1:1999877/1‑93 (MQ=255)

                                                     cAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGata                                    <  2:854655/93‑1 (MQ=255)

                                                       aaCCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAtt                                  >  1:2997784/1‑93 (MQ=255)

                                                       aaCCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAtt                                  >  1:8032657/1‑93 (MQ=255)

                                                        aCCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAttt                                 <  2:1898048/93‑1 (MQ=255)

                                                          cATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTc                               >  2:4213198/1‑93 (MQ=255)

                                                           aTGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGCCGTTGATATTTTCg                              >  1:6154677/1‑93 (MQ=255)

                                                            tGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGc                             >  1:3182531/1‑93 (MQ=255)

                                                            tGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGc                             <  2:7643782/93‑1 (MQ=255)

                                                             gAACCTCTTGCGGGGGGGGACGACGATACACTTCCGGTTCCCGTCTCTTTTCCTGGGCGTGTAACAGGGAAAAGCGGCGCTGATATTTTCgtt                            >  2:5917048/1‑91 (MQ=255)

                                                             gAACCTCGGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGcc                            <  2:7143213/93‑1 (MQ=255)

                                                                 ctctGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAAc                        >  1:4903587/1‑93 (MQ=255)

                                                                  tctGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACg                       <  2:7077290/93‑1 (MQ=255)

                                                                    tGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTc                     <  2:5076763/93‑1 (MQ=255)

                                                                    tGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTc                     >  1:3547460/1‑93 (MQ=255)

                                                                    tGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTc                     <  2:22056/93‑1 (MQ=255)

                                                                     ggCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCa                    >  1:5507002/1‑93 (MQ=255)

                                                                     ggCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCa                    <  1:7177345/93‑1 (MQ=255)

                                                                     ggCGGAGGAAGACGGCGATACTCTTCCGGTTCCTGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTATCGTCa                    <  2:2482529/93‑1 (MQ=255)

                                                                      gCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCag                   <  2:3393079/93‑1 (MQ=255)

                                                                       ggggggAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCaga                  <  1:3473970/92‑1 (MQ=255)

                                                                        gggggAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCagag                 >  1:7877881/1‑93 (MQ=255)

                                                                        gggggAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCagag                 >  1:5880520/1‑93 (MQ=255)

                                                                        gggggAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCagag                 >  1:8777209/1‑93 (MQ=255)

                                                                          gggAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCagagat               >  1:1021380/1‑91 (MQ=255)

                                                                          gggAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGt               >  2:6748977/1‑93 (MQ=255)

                                                                          gggAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGt               >  1:7582521/1‑93 (MQ=255)

                                                                           ggAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTa              >  2:9670313/1‑93 (MQ=255)

                                                                              aGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCa           <  2:5833694/93‑1 (MQ=255)

                                                                              aGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCa           <  2:1188478/93‑1 (MQ=255)

                                                                                acgacgATGCACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCAcc         <  1:4737342/93‑1 (MQ=255)

                                                                                acgacgATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCAcc         <  2:479229/93‑1 (MQ=255)

                                                                                acgacgATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCAcc         <  1:9553600/93‑1 (MQ=255)

                                                                                  gacgaTACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCga       >  2:8760792/1‑92 (MQ=255)

                                                                                  gacgaTACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGt       >  1:4882971/1‑93 (MQ=255)

                                                                                   acgaTACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTa      >  1:6418886/1‑93 (MQ=255)

                                                                                   acgaTACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTa      >  1:848453/1‑93 (MQ=255)

                                                                                     gaTACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAAt    >  1:562372/1‑93 (MQ=255)

                                                                                      aTACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATc   >  1:1161092/1‑93 (MQ=255)

                                                                                       tACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATcc  >  2:8293469/1‑93 (MQ=255)

                                                                                       tACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATcc  >  2:1745072/1‑93 (MQ=255)

                                                                                       tACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTGTCGCCTAACGTCAGAGGTAGCACCGTAATcc  >  1:9112472/1‑93 (MQ=255)

CTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCC  >  NC_000913/2017379‑2017558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCG  >  NC_000913/2017376‑2017561

AAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGGAGACGACGATACA                                                                                              >  SRR4302540.4835264/1‑94 (MQ=60)

 AGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGCCGACGATACAC                                                                                             >  SRR4302540.2126693/1‑94 (MQ=60)

 AGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACAC                                                                                             <  SRR4302540.35522/94‑1 (MQ=60)

  GCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACT                                                                                            >  SRR4302540.6532030/1‑94 (MQ=60)

   CTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTT                                                                                           <  SRR4302540.5773330/94‑1 (MQ=60)

    TTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACCGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTC                                                                                          >  SRR4302540.1432473/1‑94 (MQ=60)

    TTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTC                                                                                          <  SRR4302540.125277/94‑1 (MQ=60)

    TTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTC                                                                                          >  SRR4302540.1432474/1‑94 (MQ=60)

    TTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTC                                                                                          <  SRR4302540.6092370/94‑1 (MQ=60)

    TTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTC                                                                                          >  SRR4302540.2603513/1‑94 (MQ=60)

       AGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGG                                                                                       <  SRR4302540.6744491/94‑1 (MQ=60)

        GTGGTCAGCAGCAGGCCGCTTCCCATCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGT                                                                                      >  SRR4302540.4526859/1‑94 (MQ=60)

        GTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATTAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGT                                                                                      <  SRR4302540.3569981/94‑1 (MQ=60)

        GTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGACCCTCTGGCGGGGGAAGACGACGATACACTTCCGGT                                                                                      <  SRR4302540.2702970/94‑1 (MQ=60)

        GTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGT                                                                                      >  SRR4302540.4526858/1‑94 (MQ=60)

           GTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCC                                                                                   <  SRR4302540.4634073/94‑1 (MQ=60)

           GTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCC                                                                                   <  SRR4302540.5338146/94‑1 (MQ=60)

           GTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCC                                                                                   >  SRR4302540.3701790/1‑94 (MQ=60)

           GTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCC                                                                                   >  SRR4302540.4373681/1‑94 (MQ=60)

            TCAGCAGCAGCCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCC                                                                                  <  SRR4302540.4512892/94‑1 (MQ=60)

             CAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCG                                                                                 >  SRR4302540.7316426/1‑94 (MQ=60)

              AGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACCTCCTGTTCCCGT                                                                                >  SRR4302540.2041384/1‑94 (MQ=60)

               GCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTC                                                                               <  SRR4302540.9378330/94‑1 (MQ=60)

                CAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCT                                                                              >  SRR4302540.544708/1‑94 (MQ=60)

                 AGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTC                                                                             >  SRR4302540.2230074/1‑94 (MQ=60)

                  GCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCT                                                                            >  SRR4302540.7022352/1‑94 (MQ=60)

                  GCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCT                                                                            >  SRR4302540.3630506/1‑94 (MQ=60)

                  GCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCT                                                                            <  SRR4302540.8959320/94‑1 (MQ=60)

                   CAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAACCCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTT                                                                           <  SRR4302540.2305955/94‑1 (MQ=60)

                    AGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTT                                                                          >  SRR4302540.6668830/1‑94 (MQ=60)

                     GGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTA                                                                         >  SRR4302540.4533915/1‑94 (MQ=60)

                      GCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTAC                                                                        <  SRR4302540.6679447/94‑1 (MQ=60)

                       CCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACA                                                                       >  SRR4302540.1556156/1‑94 (MQ=60)

                        CGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAA                                                                      >  SRR4302540.253673/1‑94 (MQ=60)

                        CGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAA                                                                      <  SRR4302540.3865033/94‑1 (MQ=60)

                         GCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAG                                                                     >  SRR4302540.4667238/1‑94 (MQ=60)

                         GCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAG                                                                     >  SRR4302540.2091606/1‑94 (MQ=60)

                         GCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAG                                                                     <  SRR4302540.8084522/94‑1 (MQ=60)

                         GCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAG                                                                     <  SRR4302540.5419886/94‑1 (MQ=60)

                           TTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGG                                                                   >  SRR4302540.4374728/1‑94 (MQ=60)

                            TCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGC                                                                  >  SRR4302540.2590828/1‑94 (MQ=60)

                             CCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCG                                                                 >  SRR4302540.4530639/1‑94 (MQ=60)

                              CCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGT                                                                <  SRR4302540.6252430/94‑1 (MQ=60)

                               CAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAAGGCGTG                                                               >  SRR4302540.1408441/1‑94 (MQ=60)

                                AGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGT                                                              >  SRR4302540.4372668/1‑94 (MQ=60)

                                AGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGT                                                              <  SRR4302540.1687743/94‑1 (MQ=60)

                                 GCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTA                                                             >  SRR4302540.2450199/1‑94 (MQ=60)

                                  CAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAA                                                            >  SRR4302540.7872405/1‑94 (MQ=60)

                                   AACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAAC                                                           >  SRR4302540.4265225/1‑94 (MQ=60)

                                   AACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAAC                                                           <  SRR4302540.4044800/94‑1 (MQ=60)

                                    aaagtgaagtaaaggcactgtgacccaggaacctcggtcgggtgacGACGACGATACCTTTCCGGTTCCCGTCGCTTTACAAGGGCGTGTAACA                                                          <  SRR4302540.1460483/48‑1 (MQ=60)

                                    ACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACA                                                          <  SRR4302540.1460484/94‑1 (MQ=60)

                                     CAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAG                                                         <  SRR4302540.6988773/94‑1 (MQ=60)

                                     CAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAG                                                         <  SRR4302540.3297740/94‑1 (MQ=60)

                                     CAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAG                                                         <  SRR4302540.8697587/94‑1 (MQ=60)

                                      AGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGG                                                        >  SRR4302540.4523524/1‑94 (MQ=60)

                                        CAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCA                                                      <  SRR4302540.3268779/94‑1 (MQ=60)

                                         AAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAA                                                     >  SRR4302540.6818836/1‑94 (MQ=60)

                                         AAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAA                                                     <  SRR4302540.481550/94‑1 (MQ=60)

                                          AAGCCAACGCACAGCAAACCATTAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAAC                                                    <  SRR4302540.3183186/94‑1 (MQ=60)

                                          AAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAAC                                                    >  SRR4302540.4027955/1‑94 (MQ=60)

                                           AGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACA                                                   <  SRR4302540.5984569/94‑1 (MQ=60)

                                           AGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACA                                                   <  SRR4302540.4932966/94‑1 (MQ=60)

                                           AGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACA                                                   <  SRR4302540.4935908/94‑1 (MQ=60)

                                            GCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAG                                                  <  SRR4302540.596029/94‑1 (MQ=60)

                                             CCACCGCACAGCAAACCATGACCCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGC                                                 <  SRR4302540.3701705/94‑1 (MQ=60)

                                             CCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGC                                                 <  SRR4302540.4581381/94‑1 (MQ=60)

                                             CCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCAGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGC                                                 >  SRR4302540.4665207/1‑94 (MQ=60)

                                              CAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCG                                                <  SRR4302540.9955278/94‑1 (MQ=60)

                                              CAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCG                                                >  SRR4302540.9919227/1‑94 (MQ=60)

                                              CAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCG                                                >  SRR4302540.5603183/1‑94 (MQ=60)

                                              CAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCG                                                >  SRR4302540.8860047/1‑94 (MQ=60)

                                              CAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCG                                                <  SRR4302540.4387966/94‑1 (MQ=60)

                                               AACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGG                                               <  SRR4302540.5939305/94‑1 (MQ=60)

                                                ACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGC                                              <  SRR4302540.1205994/94‑1 (MQ=60)

                                                  GCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGT                                            >  SRR4302540.7472904/1‑94 (MQ=60)

                                                  GCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACGTCCGGTACCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGT                                            <  SRR4302540.4259569/94‑1 (MQ=60)

                                                     CAGCAAACCATGAGCCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGA                                         <  SRR4302540.6523132/94‑1 (MQ=60)

                                                     CAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGA                                         >  SRR4302540.1787997/1‑94 (MQ=60)

                                                     CAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGA                                         <  SRR4302540.6523131/94‑1 (MQ=60)

                                                      AGCAAGCCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGAT                                        <  SRR4302540.3956715/94‑1 (MQ=60)

                                                      AGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGAT                                        >  SRR4302540.2021535/1‑94 (MQ=60)

                                                         AAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATT                                     >  SRR4302540.3030814/1‑94 (MQ=60)

                                                         AAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATT                                     >  SRR4302540.8126043/1‑94 (MQ=60)

                                                         AAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGAAACAGCGGCGTTGATATT                                     >  SRR4302540.8126044/1‑94 (MQ=60)

                                                          AACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGTGAGTGTAACAGGCAACAGCGGCGTCGATAGTT                                    >  SRR4302540.1105378/1‑94 (MQ=60)

                                                             CATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCG                                 <  SRR4302540.3707018/94‑1 (MQ=60)

                                                             CATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCG                                 >  SRR4302540.9639268/1‑94 (MQ=60)

                                                             CATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCG                                 >  SRR4302540.258355/1‑94 (MQ=60)

                                                             CATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGCCGTTGATATTTTCG                                 >  SRR4302540.6223243/1‑94 (MQ=60)

                                                              ATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGC                                >  SRR4302540.3217110/1‑94 (MQ=60)

                                                              ATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGC                                >  SRR4302540.9418144/1‑94 (MQ=60)

                                                              ATGAACCTCTGGCGGGGGAAGACGACGATACACTGCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGC                                <  SRR4302540.4281084/94‑1 (MQ=60)

                                                               TGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCC                               >  SRR4302540.414782/1‑94 (MQ=60)

                                                                   CCTCTGGCGGGGGCATACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAAC                           <  SRR4302540.4506774/94‑1 (MQ=60)

                                                                   CCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAAC                           >  SRR4302540.4956349/1‑94 (MQ=60)

                                                                    CTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACG                          <  SRR4302540.8982618/94‑1 (MQ=60)

                                                                      CTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTC                        >  SRR4302540.3586209/1‑94 (MQ=60)

                                                                       TGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCA                       >  SRR4302540.5567651/1‑94 (MQ=60)

                                                                       TGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCA                       >  SRR4302540.7498445/1‑94 (MQ=60)

                                                                        GGCGGGGTAAGACGACGATACACTTCCGGTTCCCTTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAG                      <  SRR4302540.5110055/94‑1 (MQ=60)

                                                                        GGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAG                      >  SRR4302540.177417/1‑94 (MQ=60)

                                                                        GGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAG                      <  SRR4302540.7257354/94‑1 (MQ=60)

                                                                        GGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAG                      <  SRR4302540.6146352/94‑1 (MQ=60)

                                                                          GGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAG                    <  SRR4302540.3511799/94‑1 (MQ=60)

                                                                          CGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAG                    >  SRR4302540.8878863/1‑94 (MQ=60)

                                                                          CGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAG                    >  SRR4302540.5945514/1‑94 (MQ=60)

                                                                          CGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAG                    >  SRR4302540.7969890/1‑94 (MQ=60)

                                                                            GGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGT                  >  SRR4302540.7667645/1‑94 (MQ=60)

                                                                            GGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGAT                  >  SRR4302540.1031746/1‑94 (MQ=60)

                                                                                  GACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACC            >  SRR4302540.7722430/1‑94 (MQ=60)

                                                                                   ACGACGATGCACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCG           <  SRR4302540.4787909/94‑1 (MQ=60)

                                                                                   ACGACGATACACTTCCGGTTCCCGTCTCTTTACAATGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCG           >  SRR4302540.7101120/1‑94 (MQ=60)

                                                                                   ACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCG           <  SRR4302540.9666596/94‑1 (MQ=60)

                                                                                    CGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGT          >  SRR4302540.4935415/1‑94 (MQ=60)

                                                                                     GACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTA         >  SRR4302540.6490329/1‑94 (MQ=60)

                                                                                     GACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTA         <  SRR4302540.4720315/94‑1 (MQ=60)

                                                                                     GACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTA         >  SRR4302540.857451/1‑94 (MQ=60)

                                                                                      ACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAA        >  SRR4302540.874338/1‑94 (MQ=60)

                                                                                      ACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAA        <  SRR4302540.863054/94‑1 (MQ=60)

                                                                                      ACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAA        <  SRR4302540.9233390/94‑1 (MQ=60)

                                                                                      ACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAA        >  SRR4302540.7508303/1‑94 (MQ=60)

                                                                                       CGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAAT       >  SRR4302540.2874006/1‑94 (MQ=60)

                                                                                       CGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAAT       >  SRR4302540.568487/1‑94 (MQ=60)

                                                                                       CGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAAT       >  SRR4302540.568488/1‑94 (MQ=60)

                                                                                        GATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATC      >  SRR4302540.8181283/1‑94 (MQ=60)

                                                                                        GATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATC      >  SRR4302540.9708758/1‑94 (MQ=60)

                                                                                        GATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATC      >  SRR4302540.1173156/1‑94 (MQ=60)

                                                                                         ATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTGTCGCCTAACGTCAGAGGTAGCACCGTAATCC     >  SRR4302540.9221990/1‑94 (MQ=60)

                                                                                           ACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGC   >  SRR4302540.2126325/1‑94 (MQ=60)

                                                                                            CACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCG  >  SRR4302540.79330/1‑94 (MQ=60)

AAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCG  >  NC_000913/2017376‑2017561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`