Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,028,708 A→G V78A (GTG→GCG)  vsr ← DNA mismatch endonuclease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,028,7080AG99.6% 808.9 / ‑5.0 252V78A (GTG→GCG) vsrDNA mismatch endonuclease
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (122/129);  total (122/130)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.23e-01

Only 100 of 257 total aligned reads displayed.

CCGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCACTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGACGT  >  NC_000913/2028618‑2028797
                                                                                          |                                                                                         
ccGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCt                                                                                         <  2:6802536/93‑1 (MQ=255)
 cGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCtt                                                                                        >  1:7812780/1‑93 (MQ=255)
  gAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCttt                                                                                       >  2:9682147/1‑93 (MQ=255)
  gAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCttt                                                                                       <  2:4694831/93‑1 (MQ=255)
   aGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTa                                                                                      >  1:6552674/1‑93 (MQ=255)
    gTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAGCATTTTTCCCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTaa                                                                                     <  1:7065692/93‑1 (MQ=255)
     ttCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTaaa                                                                                    >  2:3802007/1‑93 (MQ=255)
     ttCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTaaa                                                                                    <  2:7917075/93‑1 (MQ=255)
       ccTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACa                                                                                  >  2:19126/1‑93 (MQ=255)
       ccTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGACAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACa                                                                                  >  1:5338620/1‑93 (MQ=255)
        cTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAg                                                                                 >  2:9574517/1‑93 (MQ=255)
         tGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGa                                                                                <  1:3147155/93‑1 (MQ=255)
           cAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATa                                                                              >  1:1662988/1‑93 (MQ=255)
             aGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGc                                                                            <  1:5900430/93‑1 (MQ=255)
                gACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTCCTCCAGCCAGACCTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGt                                                                         <  1:9667534/93‑1 (MQ=255)
                 aCTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGtg                                                                        >  1:5599463/1‑93 (MQ=255)
                  cTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGtga                                                                       <  1:2869747/93‑1 (MQ=255)
                  cTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGtga                                                                       <  2:8010672/93‑1 (MQ=255)
                  cTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGtga                                                                       <  1:1575046/93‑1 (MQ=255)
                      tatCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGtgatgat                                                                   >  1:4685638/1‑93 (MQ=255)
                       atCGCGGCGCGCGCTCTCAACATTTTTACCTATCTTCGCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGtgatgatg                                                                  <  2:2602251/93‑1 (MQ=255)
                        tCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGtgatgatga                                                                 >  2:925986/1‑93 (MQ=255)
                        tCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGtgatgatga                                                                 <  1:9728806/93‑1 (MQ=255)
                          gcggcgATCGCGCTCACCATTTTTACCTATCTTCTCCGGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGatgatgatg                                                               <  2:5228864/93‑1 (MQ=255)
                          gcggcgATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGatgatgatg                                                               <  2:4982199/93‑1 (MQ=255)
                          gcggcgATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGatgatgatg                                                               >  1:6180415/1‑93 (MQ=255)
                           cggcgATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGc                                                              <  2:9200938/93‑1 (MQ=255)
                           cggcgATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGc                                                              >  1:54411/1‑93 (MQ=255)
                             gcgATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCa                                                            >  1:2555666/1‑93 (MQ=255)
                              cgATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAg                                                           >  2:7078692/1‑93 (MQ=255)
                              cgATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAg                                                           <  2:2463158/93‑1 (MQ=255)
                               gATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGa                                                          >  2:8620534/1‑93 (MQ=255)
                                gtCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGaa                                                         <  1:6306629/92‑1 (MQ=255)
                                aTCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGaa                                                         >  1:1625206/1‑93 (MQ=255)
                                aTCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGaa                                                         >  1:9870721/1‑93 (MQ=255)
                                aTCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGaa                                                         >  2:2501072/1‑93 (MQ=255)
                                 tCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAag                                                        <  2:6755777/93‑1 (MQ=255)
                                  cgcgCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAagc                                                       <  2:8956068/93‑1 (MQ=255)
                                   gcgcTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAagca                                                      <  2:755488/93‑1 (MQ=255)
                                    cgcTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAagcag                                                     >  2:8412933/1‑93 (MQ=255)
                                     gcTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAagcagc                                                    <  1:8479425/93‑1 (MQ=255)
                                      cTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGcc                                                   >  1:2707573/1‑93 (MQ=255)
                                       tCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCa                                                  <  2:7634709/93‑1 (MQ=255)
                                        cAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCAt                                                 >  2:3050393/1‑93 (MQ=255)
                                        cAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCAt                                                 >  1:9080161/1‑93 (MQ=255)
                                        cAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATCCCAGAAGCAGCCAt                                                 >  1:4062045/1‑93 (MQ=255)
                                         aaCATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATg                                                <  1:8641448/93‑1 (MQ=255)
                                          aCATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATgg                                               <  2:9364935/93‑1 (MQ=255)
                                           cATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGGGATGATGATGCCAGAAGCAGCCATggg                                              >  1:7251669/1‑93 (MQ=255)
                                             tttttACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTa                                            >  2:8314869/1‑93 (MQ=255)
                                                ttACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAat                                         <  1:1422920/93‑1 (MQ=255)
                                                ttACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAat                                         <  2:1782256/93‑1 (MQ=255)
                                                ttACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAat                                         <  2:2441231/93‑1 (MQ=255)
                                                ttACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAat                                         >  2:4337036/1‑93 (MQ=255)
                                                 tACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAata                                        <  1:7026268/93‑1 (MQ=255)
                                                 tACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAata                                        >  1:5044372/1‑93 (MQ=255)
                                                  tccTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAatat                                       <  1:2602982/92‑1 (MQ=255)
                                                  aCCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAatat                                       <  1:4154583/93‑1 (MQ=255)
                                                   ccTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATc                                      <  2:5750556/93‑1 (MQ=255)
                                                    cTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCa                                     <  2:8180217/93‑1 (MQ=255)
                                                    cTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCa                                     >  2:3401367/1‑93 (MQ=255)
                                                     tATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCAc                                    >  2:2632198/1‑93 (MQ=255)
                                                     tATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCAc                                    >  1:4826520/1‑93 (MQ=255)
                                                     tATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGACAGAAGCAGCCATGGGTAAATATCAc                                    >  1:1768066/1‑93 (MQ=255)
                                                      aTCTTCTCCAGCCAGAACTCGTTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACg                                   <  1:4572962/93‑1 (MQ=255)
                                                      aTCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACg                                   >  2:7352873/1‑93 (MQ=255)
                                                      aTCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACg                                   >  1:4482543/1‑93 (MQ=255)
                                                       tcttctCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGc                                  >  1:7150947/1‑93 (MQ=255)
                                                       tcttctCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGc                                  >  1:9127696/1‑93 (MQ=255)
                                                       tcttctCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGc                                  >  1:3935479/1‑93 (MQ=255)
                                                        cttctCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCa                                 >  1:10008612/1‑93 (MQ=255)
                                                          tctcCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGc                               <  1:7304786/93‑1 (MQ=255)
                                                               agccagAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGatat                          >  2:2984525/1‑93 (MQ=255)
                                                               agccagAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGatat                          >  1:3059915/1‑93 (MQ=255)
                                                                gccagAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATAtt                         <  1:3620090/93‑1 (MQ=255)
                                                                 ccagAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATtc                        <  2:2062455/93‑1 (MQ=255)
                                                                 ccagAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATtc                        <  1:925986/93‑1 (MQ=255)
                                                                  cagAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATtca                       <  2:1306678/93‑1 (MQ=255)
                                                                  cagAACTCGGTTCGAGCCGCAGGCGCTTAAAACAGATAGCAGTGATGATGATGCAAGAAGCAGCAATGGGTAAATATCACGCAGCGATATtca                       <  2:5436284/93‑1 (MQ=255)
                                                                   agAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATtcat                      >  2:314045/1‑93 (MQ=255)
                                                                   agAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATtcat                      >  1:2076732/1‑93 (MQ=255)
                                                                     aaCTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGGTGATGCCAGAAGCAGACATGGGTAAATATCACGCAGCGATATtcatga                    >  2:9938884/1‑91 (MQ=255)
                                                                     aaCTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATtcatca                    >  1:1120253/1‑93 (MQ=255)
                                                                     aaCTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATtcatca                    >  2:2528113/1‑93 (MQ=255)
                                                                     aaCTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATtcatca                    >  1:774044/1‑93 (MQ=255)
                                                                         cGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGa                <  1:197720/93‑1 (MQ=255)
                                                                          ggTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGAc               >  1:2355461/1‑93 (MQ=255)
                                                                           gTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACa              <  2:8895821/93‑1 (MQ=255)
                                                                           gTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACa              <  2:4457358/93‑1 (MQ=255)
                                                                            ttCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACaa             <  1:1531378/93‑1 (MQ=255)
                                                                             tCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACaaa            <  1:67464/93‑1 (MQ=255)
                                                                              cGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACaaaa           <  2:6088827/93‑1 (MQ=255)
                                                                               gAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAAt          >  1:1851609/1‑93 (MQ=255)
                                                                                aGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATc         >  1:124977/1‑93 (MQ=255)
                                                                                 gTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATcc        >  1:2421494/1‑93 (MQ=255)
                                                                                    gCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGa     <  1:424548/93‑1 (MQ=255)
                                                                                    gCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGa     >  2:4363977/1‑93 (MQ=255)
                                                                                     cAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGAc    <  2:5925460/93‑1 (MQ=255)
                                                                                      aGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGACg   >  2:9630961/1‑93 (MQ=255)
                                                                                       ggCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGTGATATTCATCAACGACAAAATCCGGACGt  >  1:5618176/1‑93 (MQ=255)
                                                                                          |                                                                                         
CCGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCACTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGACGT  >  NC_000913/2028618‑2028797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCCGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCACTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGACGTCCGG  >  NC_000913/2028616‑2028801
                                                                                            |                                                                                             
AGCCGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGCACTCGGTTCGAGTCGCAGGCGC                                                                                              >  SRR4302540.4036836/1‑94 (MQ=60)
AGCCGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCCGGCGC                                                                                              >  SRR4302540.4036831/1‑94 (MQ=60)
AGCCGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGC                                                                                              <  SRR4302540.1944481/94‑1 (MQ=60)
AGCCGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGC                                                                                              >  SRR4302540.5395564/1‑94 (MQ=60)
AGCCGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGC                                                                                              >  SRR4302540.2652745/1‑94 (MQ=60)
AGCCGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGC                                                                                              >  SRR4302540.4036837/1‑94 (MQ=60)
AGCCGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGC                                                                                              >  SRR4302540.8938201/1‑94 (MQ=60)
 GCCGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCT                                                                                             <  SRR4302540.1075728/94‑1 (MQ=60)
 GCCGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCT                                                                                             >  SRR4302540.6394582/1‑94 (MQ=60)
 GCCGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCT                                                                                             <  SRR4302540.2450253/94‑1 (MQ=60)
 GCCGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCT                                                                                             >  SRR4302540.1320236/1‑94 (MQ=60)
  CCGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTT                                                                                            >  SRR4302540.7904080/1‑94 (MQ=60)
   CGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTT                                                                                           >  SRR4302540.5293179/1‑94 (MQ=60)
    GAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTA                                                                                          >  SRR4302540.6625347/1‑94 (MQ=60)
      GTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAGCATTTTTCCCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAA                                                                                        <  SRR4302540.7144272/94‑1 (MQ=60)
        TCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGACAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACA                                                                                      >  SRR4302540.5397149/1‑94 (MQ=60)
         CCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAG                                                                                     >  SRR4302540.1245437/1‑94 (MQ=60)
           TGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGAT                                                                                   >  SRR4302540.9217241/1‑94 (MQ=60)
           TGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGAT                                                                                   <  SRR4302540.3181440/94‑1 (MQ=60)
           TGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGAT                                                                                   <  SRR4302540.7029002/94‑1 (MQ=60)
            GCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATA                                                                                  >  SRR4302540.1681096/1‑94 (MQ=60)
            GCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTCACACAGATA                                                                                  >  SRR4302540.1681095/1‑94 (MQ=60)
             CAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAG                                                                                 <  SRR4302540.3982761/94‑1 (MQ=60)
               AGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCA                                                                               <  SRR4302540.5965588/94‑1 (MQ=60)
                  GACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTG                                                                            >  SRR4302540.5661068/1‑94 (MQ=60)
                  GACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTCCTCCAGCCAGACCTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTG                                                                            <  SRR4302540.9781323/94‑1 (MQ=60)
                    CTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGAT                                                                          <  SRR4302540.2900912/94‑1 (MQ=60)
                    CTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGAT                                                                          <  SRR4302540.1592284/94‑1 (MQ=60)
                    CTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGAT                                                                          <  SRR4302540.7398807/94‑1 (MQ=60)
                     TGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATG                                                                         >  SRR4302540.7152236/1‑94 (MQ=60)
                     TGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATG                                                                         >  SRR4302540.1582026/1‑94 (MQ=60)
                       ATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGAT                                                                       <  SRR4302540.539703/94‑1 (MQ=60)
                       ATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGAT                                                                       >  SRR4302540.4735756/1‑94 (MQ=60)
                         ATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGA                                                                     <  SRR4302540.6264383/94‑1 (MQ=60)
                          TCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGAT                                                                    <  SRR4302540.9843057/94‑1 (MQ=60)
                          TCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGAT                                                                    <  SRR4302540.9773724/94‑1 (MQ=60)
                          TCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGAT                                                                    <  SRR4302540.9340303/94‑1 (MQ=60)
                           CGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATG                                                                   >  SRR4302540.6249259/1‑94 (MQ=60)
                           CGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATG                                                                   <  SRR4302540.8765980/94‑1 (MQ=60)
                           CGCGGCAATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTGCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATG                                                                   <  SRR4302540.239174/94‑1 (MQ=60)
                            GCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGC                                                                  >  SRR4302540.54954/1‑94 (MQ=60)
                             CGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCC                                                                 >  SRR4302540.4158219/1‑94 (MQ=60)
                              GGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCA                                                                >  SRR4302540.2583200/1‑94 (MQ=60)
                                CGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGA                                                              <  SRR4302540.8697086/94‑1 (MQ=60)
                                 GATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGCA                                                             >  SRR4302540.2033954/1‑94 (MQ=60)
                                 GATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAA                                                             <  SRR4302540.4757518/94‑1 (MQ=60)
                                 GATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAA                                                             >  SRR4302540.9986002/1‑94 (MQ=60)
                                 GATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAA                                                             >  SRR4302540.1643003/1‑94 (MQ=60)
                                  GTCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAG                                                            <  SRR4302540.6376861/94‑1 (MQ=60)
                                  ATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAG                                                            <  SRR4302540.3001330/94‑1 (MQ=60)
                                  ATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAG                                                            >  SRR4302540.3106035/1‑94 (MQ=60)
                                  ATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAG                                                            >  SRR4302540.6824015/1‑94 (MQ=60)
                                  ATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAG                                                            >  SRR4302540.1774871/1‑94 (MQ=60)
                                   TCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGC                                                           >  SRR4302540.1470843/1‑94 (MQ=60)
                                    CGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCA                                                          <  SRR4302540.1115934/94‑1 (MQ=60)
                                     GCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAG                                                         <  SRR4302540.9856447/94‑1 (MQ=60)
                                       GCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCC                                                       >  SRR4302540.2736774/1‑94 (MQ=60)
                                       GCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCC                                                       <  SRR4302540.8577914/94‑1 (MQ=60)
                                       GCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGGCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCC                                                       >  SRR4302540.6630049/1‑94 (MQ=60)
                                        CTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCA                                                      >  SRR4302540.1605123/1‑94 (MQ=60)
                                         TCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCAT                                                     >  SRR4302540.9189241/1‑94 (MQ=60)
                                         TCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCAT                                                     <  SRR4302540.4494985/94‑1 (MQ=60)
                                         TCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCAT                                                     >  SRR4302540.2992653/1‑94 (MQ=60)
                                         TCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATCCCAGAAGCAGCCAT                                                     >  SRR4302540.4105842/1‑94 (MQ=60)
                                          CAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATG                                                    >  SRR4302540.1952754/1‑94 (MQ=60)
                                           AACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGG                                                   <  SRR4302540.4886388/94‑1 (MQ=60)
                                           AACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGG                                                   <  SRR4302540.8741825/94‑1 (MQ=60)
                                            ACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGG                                                  >  SRR4302540.7332588/1‑94 (MQ=60)
                                            ACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGGGATGATGATGCCAGAAGCAGCCATGGG                                                  >  SRR4302540.7332589/1‑94 (MQ=60)
                                             CATTTTTGCCTATTTGCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGT                                                 <  SRR4302540.5662116/94‑1 (MQ=60)
                                              ATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTA                                                <  SRR4302540.4561291/94‑1 (MQ=60)
                                               TTTTTACCTATCTTCTCCAGCCCGAACTCGGTTCCAGCCGCAGGCACTTTAAACAGATCGCAGTGATGATGATGCCAGAAGCAGCCATGGGCAA                                               >  SRR4302540.4223153/1‑94 (MQ=60)
                                                TTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAA                                              <  SRR4302540.2541893/94‑1 (MQ=60)
                                                TTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAA                                              >  SRR4302540.4085299/1‑94 (MQ=60)
                                                 TTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAAT                                             <  SRR4302540.7160611/94‑1 (MQ=60)
                                                 TTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAAT                                             <  SRR4302540.3205610/94‑1 (MQ=60)
                                                 TTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAAT                                             <  SRR4302540.3205609/94‑1 (MQ=60)
                                                  TTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATA                                            <  SRR4302540.1438324/94‑1 (MQ=60)
                                                  TTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATA                                            <  SRR4302540.1438323/94‑1 (MQ=60)
                                                  TTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATA                                            >  SRR4302540.5098932/1‑94 (MQ=60)
                                                  TTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATA                                            <  SRR4302540.4144321/94‑1 (MQ=60)
                                                   TACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATAT                                           <  SRR4302540.7104322/94‑1 (MQ=60)
                                                   TACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATAT                                           <  SRR4302540.2695805/94‑1 (MQ=60)
                                                   TACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATAT                                           >  SRR4302540.9841784/1‑94 (MQ=60)
                                                   TACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATAT                                           >  SRR4302540.9372498/1‑94 (MQ=60)
                                                   TACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATAT                                           <  SRR4302540.1755900/94‑1 (MQ=60)
                                                    TCCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATC                                          <  SRR4302540.2631058/94‑1 (MQ=60)
                                                    ACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATC                                          >  SRR4302540.2665094/1‑94 (MQ=60)
                                                    ACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATC                                          <  SRR4302540.4199586/94‑1 (MQ=60)
                                                      CTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCAC                                        >  SRR4302540.2888099/1‑94 (MQ=60)
                                                      CTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCAC                                        >  SRR4302540.4878206/1‑94 (MQ=60)
                                                      CTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGACAGAAGCAGCCATGGGTAAATATCAC                                        >  SRR4302540.1787249/1‑94 (MQ=60)
                                                       TATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACG                                       >  SRR4302540.4531159/1‑94 (MQ=60)
                                                        ATCTTCTCCAGCCAGAACTCGTTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGC                                      <  SRR4302540.4622308/94‑1 (MQ=60)
                                                        ATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGC                                      >  SRR4302540.7230636/1‑94 (MQ=60)
                                                        ATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGC                                      >  SRR4302540.9237410/1‑94 (MQ=60)
                                                        ATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGC                                      >  SRR4302540.3978015/1‑94 (MQ=60)
                                                        ATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGAAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGG                                      >  SRR4302540.7116132/1‑94 (MQ=60)
                                                         TCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCA                                     >  SRR4302540.10125164/1‑94 (MQ=60)
                                                          CTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAG                                    <  SRR4302540.6071512/94‑1 (MQ=60)
                                                          CTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAG                                    <  SRR4302540.5867909/94‑1 (MQ=60)
                                                           TTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGC                                   >  SRR4302540.8872643/1‑94 (MQ=60)
                                                           TTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCACCCATGGGTAAATATCACGCAGC                                   <  SRR4302540.1866358/94‑1 (MQ=60)
                                                            TCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCG                                  <  SRR4302540.8032647/94‑1 (MQ=60)
                                                            TCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCG                                  <  SRR4302540.7386328/94‑1 (MQ=60)
                                                              CCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGAT                                <  SRR4302540.824317/94‑1 (MQ=60)
                                                                CAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATAT                              >  SRR4302540.3093484/1‑94 (MQ=60)
                                                                CAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATAT                              >  SRR4302540.934221/1‑94 (MQ=60)
                                                                CAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATAT                              <  SRR4302540.4361541/94‑1 (MQ=60)
                                                                  GCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTC                            <  SRR4302540.3659695/94‑1 (MQ=60)
                                                                  GCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTC                            <  SRR4302540.7367829/94‑1 (MQ=60)
                                                                   CCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCA                           <  SRR4302540.4648870/94‑1 (MQ=60)
                                                                   CCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCA                           >  SRR4302540.7366183/1‑94 (MQ=60)
                                                                   CCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCA                           <  SRR4302540.935739/94‑1 (MQ=60)
                                                                    CAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCAT                          >  SRR4302540.2099051/1‑94 (MQ=60)
                                                                    CAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCAT                          <  SRR4302540.504165/94‑1 (MQ=60)
                                                                     AGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATC                         >  SRR4302540.5623262/1‑94 (MQ=60)
                                                                      GAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCA                        >  SRR4302540.782270/1‑94 (MQ=60)
                                                                      GAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCA                        <  SRR4302540.2394802/94‑1 (MQ=60)
                                                                      GAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCA                        >  SRR4302540.1131877/1‑94 (MQ=60)
                                                                      GAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCA                        <  SRR4302540.5578475/94‑1 (MQ=60)
                                                                       AACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAA                       >  SRR4302540.4264872/1‑94 (MQ=60)
                                                                       AACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAA                       <  SRR4302540.3889745/94‑1 (MQ=60)
                                                                        ACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAAC                      <  SRR4302540.5840841/94‑1 (MQ=60)
                                                                         CTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACG                     <  SRR4302540.5312404/94‑1 (MQ=60)
                                                                         CTCGGTTCGAGTCGCAGGCGCTGTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACG                     >  SRR4302540.1861664/1‑94 (MQ=60)
                                                                           CGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGAC                   <  SRR4302540.199677/94‑1 (MQ=60)
                                                                           CGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGAC                   >  SRR4302540.2380832/1‑94 (MQ=60)
                                                                            GGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGAGATATTCATCCACGACA                  >  SRR4302540.9850456/1‑94 (MQ=60)
                                                                              TTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAA                >  SRR4302540.1260395/1‑94 (MQ=60)
                                                                              TTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAA                <  SRR4302540.1548336/94‑1 (MQ=60)
                                                                               TCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAA               <  SRR4302540.68144/94‑1 (MQ=60)
                                                                                CGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAAT              >  SRR4302540.1871916/1‑94 (MQ=60)
                                                                                CGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAAT              <  SRR4302540.7440551/94‑1 (MQ=60)
                                                                                 GAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATC             >  SRR4302540.126303/1‑94 (MQ=60)
                                                                                 GAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATC             <  SRR4302540.1593782/94‑1 (MQ=60)
                                                                                  AGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCC            >  SRR4302540.2447587/1‑94 (MQ=60)
                                                                                    TCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGG          <  SRR4302540.7881872/94‑1 (MQ=60)
                                                                                    TCGCAGGCGCTTTAAACAGAAAGCAGTAATGATGATGCCAGAAGCAGCCATGAGTACATATCACGCAGCGATATTCATCAACGACAAAATCCGG          <  SRR4302540.2130496/94‑1 (MQ=60)
                                                                                     CGTAGGCGCTTTAAACAGATAGCAGTGATGATGACGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGA         <  SRR4302540.6286845/94‑1 (MQ=60)
                                                                                     CGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGA         <  SRR4302540.2586304/94‑1 (MQ=60)
                                                                                     CGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGA         <  SRR4302540.2586305/94‑1 (MQ=60)
                                                                                     CGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGA         >  SRR4302540.4677438/1‑94 (MQ=60)
                                                                                      GCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGAC        <  SRR4302540.429018/94‑1 (MQ=60)
                                                                                      GCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGAC        >  SRR4302540.2025871/1‑94 (MQ=60)
                                                                                      GCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGAC        <  SRR4302540.6433607/94‑1 (MQ=60)
                                                                                        AGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGTGATATTCATCAACGACAAAATCCGGACGT      >  SRR4302540.5679997/1‑94 (MQ=60)
                                                                                          GCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGACGTCC    <  SRR4302540.3775962/94‑1 (MQ=60)
                                                                                           CGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGACGTCCG   <  SRR4302540.873363/94‑1 (MQ=60)
                                                                                            GCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGACGTCCGG  <  SRR4302540.1132525/94‑1 (MQ=60)
                                                                                            GCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGACGTCCGG  <  SRR4302540.1499882/94‑1 (MQ=60)
                                                                                            |                                                                                             
AGCCGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCACTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGACGTCCGG  >  NC_000913/2028616‑2028801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑