Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,017,469	G→A	T354T (ACG→ACA)	fliK →	flagellar hook‑length control protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,017,469	0	G	A	99.6%	784.3 / NA	223	T354T (ACG→ACA)	fliK	flagellar hook‑length control protein
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (92/130); minor base C (0/1); total (92/131)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 228 total aligned reads displayed.

TTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATC  >  NC_000913/2017380‑2017557

tttAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACtt                                                                                       <  2:3182193/93‑1 (MQ=255)

   aGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGACCCTCTGGCGGGAGAAGACGACGATACCCTTCCg                                                                                    <  2:1738381/93‑1 (MQ=255)

     tGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGt                                                                                  <  2:4267870/93‑1 (MQ=255)

     tGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGt                                                                                  <  2:335746/93‑1 (MQ=255)

      ggTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGtt                                                                                 <  2:4185367/93‑1 (MQ=255)

       gTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTc                                                                                <  1:4263794/93‑1 (MQ=255)

       gTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGGTc                                                                                >  2:2663497/1‑93 (MQ=255)

        tCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTcc                                                                               <  1:4449092/93‑1 (MQ=255)

        tCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTcc                                                                               <  1:5439710/93‑1 (MQ=255)

         cagcagcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTccc                                                                              <  1:3740284/93‑1 (MQ=255)

         cagcagcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTccc                                                                              <  1:588779/93‑1 (MQ=255)

          agcagcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCg                                                                             >  2:4481290/1‑93 (MQ=255)

          agcagcagGCCGCTCCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGACCCTCGGGCGGGGGAAGACGACGATACACTTCCGGTTCCCg                                                                             <  2:3547349/93‑1 (MQ=255)

           gcagcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGt                                                                            >  1:2753193/1‑93 (MQ=255)

           gcagcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGt                                                                            <  2:4927899/93‑1 (MQ=255)

            cagcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGtc                                                                           <  2:4561155/93‑1 (MQ=255)

             agcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGATGATACACTTCCGGTTCCCGtct                                                                          >  1:4244933/1‑93 (MQ=255)

              gcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGtctc                                                                         >  2:1211884/1‑93 (MQ=255)

              gcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGtctc                                                                         >  1:5060903/1‑93 (MQ=255)

              gcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGtctc                                                                         >  1:1142669/1‑93 (MQ=255)

               cagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCGCGTctct                                                                        >  1:2204701/1‑93 (MQ=255)

                agGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCtt                                                                       <  2:1147469/93‑1 (MQ=255)

                agGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCtt                                                                       >  1:1523876/1‑93 (MQ=255)

                 ggCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCttt                                                                      >  2:5431658/1‑93 (MQ=255)

                      cTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAg                                                                 >  2:1767356/1‑93 (MQ=255)

                      cTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAg                                                                 <  1:1894587/93‑1 (MQ=255)

                      cTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAg                                                                 >  1:4124686/1‑93 (MQ=255)

                        tCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAggg                                                               <  2:3187857/93‑1 (MQ=255)

                        tCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAggg                                                               <  1:944789/93‑1 (MQ=255)

                        tCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAggg                                                               >  1:5532461/1‑93 (MQ=255)

                           cagcaacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCgt                                                            >  1:2318687/1‑93 (MQ=255)

                           cagcaacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCgt                                                            <  1:3092926/93‑1 (MQ=255)

                            agcaacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCgtg                                                           <  1:5059751/93‑1 (MQ=255)

                            agcaacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCgtg                                                           <  1:5258399/93‑1 (MQ=255)

                             gcaacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCgtgt                                                          <  1:5404176/93‑1 (MQ=255)

                              caacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTa                                                         >  2:4279340/1‑93 (MQ=255)

                                acagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAAc                                                       >  2:2508261/1‑93 (MQ=255)

                                acagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAAc                                                       >  2:1911635/1‑93 (MQ=255)

                                   gcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAgg                                                    >  2:5200914/1‑93 (MQ=255)

                                   gcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAgg                                                    <  1:5814400/93‑1 (MQ=255)

                                    caaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGc                                                   >  2:3854232/1‑93 (MQ=255)

                                     aaaGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCa                                                  >  2:2276503/1‑93 (MQ=255)

                                      aaGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTCCAAGGGCGTGTAACAGGCaa                                                 >  2:3927281/1‑93 (MQ=255)

                                       aGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAAc                                                <  1:3938162/93‑1 (MQ=255)

                                       aGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAAc                                                <  1:1969239/93‑1 (MQ=255)

                                       aGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAAc                                                <  1:719346/93‑1 (MQ=255)

                                         ccAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAg                                              <  2:1043760/93‑1 (MQ=255)

                                          cAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAgc                                             <  2:4773209/93‑1 (MQ=255)

                                          cAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAgc                                             <  1:4658610/93‑1 (MQ=255)

                                           aaCGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAgcg                                            <  2:1677982/93‑1 (MQ=255)

                                            aCGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAgcgg                                           <  2:545699/93‑1 (MQ=255)

                                            aCGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGGGTAATAGGCAACAgcgg                                           >  1:2471048/1‑93 (MQ=255)

                                              gCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACCAGGGCGTGTAACAGGCAACAgcggcg                                         >  1:3801246/1‑93 (MQ=255)

                                                acaGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTTACAGGCAACAgcggcgct                                       >  2:2291469/1‑91 (MQ=255)

                                                 caGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTg                                      <  2:247124/93‑1 (MQ=255)

                                                  aGCAAACCATGATCCTCTGGCGGGGGAAGACGACGATACACTGCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGa                                     <  1:4352578/93‑1 (MQ=255)

                                                  aGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGa                                     >  2:5787761/1‑93 (MQ=255)

                                                   gCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGat                                    <  2:2739607/93‑1 (MQ=255)

                                                   gCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGat                                    <  1:4491778/93‑1 (MQ=255)

                                                    cAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGata                                   <  1:4758079/93‑1 (MQ=255)

                                                    cAAACCATGAACCTCTGGCGGGGGAAGAAGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGata                                   <  2:4603733/93‑1 (MQ=255)

                                                      aaCCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAtt                                 >  2:3115318/1‑93 (MQ=255)

                                                      aaCCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAtt                                 >  1:2164837/1‑93 (MQ=255)

                                                      aaCCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAtt                                 >  1:1075530/1‑93 (MQ=255)

                                                      aaCCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAtt                                 >  1:4037639/1‑93 (MQ=255)

                                                       aCCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAttt                                >  2:3286706/1‑93 (MQ=255)

                                                       aCCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTATCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAttt                                >  1:3613655/1‑93 (MQ=255)

                                                        ccATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAtttt                               <  1:5978675/93‑1 (MQ=255)

                                                        ccATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAtttt                               >  1:5649784/1‑93 (MQ=255)

                                                         cATGAGCCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTc                              <  1:3025796/93‑1 (MQ=255)

                                                         cATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTc                              <  1:1405268/93‑1 (MQ=255)

                                                          aTGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCg                             <  2:6119537/93‑1 (MQ=255)

                                                           tGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGc                            <  1:5192677/93‑1 (MQ=255)

                                                            gAACCTCTGGCGGGGTAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGcc                           <  2:2782895/93‑1 (MQ=255)

                                                              aCCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTa                         >  2:3050366/1‑93 (MQ=255)

                                                               ccTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTaa                        >  1:3704376/1‑93 (MQ=255)

                                                                 cctGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACg                      <  1:17348/92‑1 (MQ=255)

                                                                     gCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCag                  <  1:2575356/93‑1 (MQ=255)

                                                                      cGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCaga                 >  2:4040586/1‑93 (MQ=255)

                                                                      cGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCaga                 <  1:1696533/93‑1 (MQ=255)

                                                                       gggggAAGCCGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCagag                <  2:1574544/93‑1 (MQ=255)

                                                                       gggggAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCagag                <  2:6185934/93‑1 (MQ=255)

                                                                        ggggAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAgg               >  2:90222/1‑93 (MQ=255)

                                                                        ggggAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAgg               <  1:2643294/93‑1 (MQ=255)

                                                                        ggggAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAgg               <  1:3220718/93‑1 (MQ=255)

                                                                        ggggAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAgg               <  2:5002799/93‑1 (MQ=255)

                                                                        ggggAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAgg               >  2:3916012/1‑93 (MQ=255)

                                                                          ggAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTa             <  1:5934344/93‑1 (MQ=255)

                                                                          ggAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTa             <  2:1727817/93‑1 (MQ=255)

                                                                              gacgacGATACACTTCCGGTTCTCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCAc         <  1:5656977/93‑1 (MQ=255)

                                                                              gacgacGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCAc         <  1:6138146/93‑1 (MQ=255)

                                                                              gacgacGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCAc         <  2:3688154/93‑1 (MQ=255)

                                                                              gacgacGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCAc         >  2:6294683/1‑93 (MQ=255)

                                                                                cgacgaTACACTGCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCg       >  1:3405517/1‑93 (MQ=255)

                                                                                   cgaTACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTaa    <  2:4429310/93‑1 (MQ=255)

                                                                                   cgaTACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTaa    <  1:2098507/93‑1 (MQ=255)

                                                                                    gaTACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAAt   >  2:5289424/1‑93 (MQ=255)

                                                                                     aTACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATc  <  1:664618/93‑1 (MQ=255)

                                                                                     aTACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATc  <  2:5152872/93‑1 (MQ=255)

                                                                                     aTACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATc  <  2:1874943/93‑1 (MQ=255)

TTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATC  >  NC_000913/2017380‑2017557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGT  >  NC_000913/2017376‑2017562

AAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACA                                                                                               >  SRR4302533.4446722/1‑94 (MQ=60)

AAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACA                                                                                               <  SRR4302533.832445/94‑1 (MQ=60)

 AGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACAC                                                                                              >  SRR4302533.1240411/1‑94 (MQ=60)

  GCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACT                                                                                             <  SRR4302533.1180302/94‑1 (MQ=60)

  GCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACT                                                                                             <  SRR4302533.6219236/94‑1 (MQ=60)

    TTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTC                                                                                           >  SRR4302533.5660008/1‑94 (MQ=60)

    TTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTC                                                                                           >  SRR4302533.3808606/1‑94 (MQ=60)

     TTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCC                                                                                          <  SRR4302533.2289151/94‑1 (MQ=60)

     TTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCC                                                                                          <  SRR4302533.5095496/94‑1 (MQ=60)

     TTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCC                                                                                          >  SRR4302533.1738300/1‑94 (MQ=60)

        GTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGT                                                                                       >  SRR4302533.4720075/1‑94 (MQ=60)

          GGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTC                                                                                     <  SRR4302533.5835305/94‑1 (MQ=60)

          GGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTC                                                                                     >  SRR4302533.430640/1‑94 (MQ=60)

           GTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCC                                                                                    <  SRR4302533.4289479/94‑1 (MQ=60)

           GTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCC                                                                                    <  SRR4302533.2501384/94‑1 (MQ=60)

            TCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCC                                                                                   <  SRR4302533.5472469/94‑1 (MQ=60)

            TCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCC                                                                                   <  SRR4302533.4475999/94‑1 (MQ=60)

             CAGCAGCAGGCCGCTTCCCAGGAACAGCAACGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCG                                                                                  <  SRR4302533.825329/94‑1 (MQ=60)

             CAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCG                                                                                  <  SRR4302533.3763223/94‑1 (MQ=60)

             CAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCG                                                                                  >  SRR4302533.5355904/1‑94 (MQ=60)

             CAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCG                                                                                  >  SRR4302533.5995330/1‑94 (MQ=60)

             CAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCG                                                                                  <  SRR4302533.592259/94‑1 (MQ=60)

              AGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGT                                                                                 >  SRR4302533.2770076/1‑94 (MQ=60)

               GCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTC                                                                                <  SRR4302533.408200/94‑1 (MQ=60)

                CAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGATGATACACTTCCGGTTCCCGTCT                                                                               >  SRR4302533.4270564/1‑94 (MQ=60)

                CAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCT                                                                               <  SRR4302533.2016299/94‑1 (MQ=60)

                CAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCT                                                                               <  SRR4302533.3060775/94‑1 (MQ=60)

                CAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGAAGATACACTTCCCGTTCCCGTCT                                                                               >  SRR4302533.4004633/1‑94 (MQ=60)

                 AGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTC                                                                              >  SRR4302533.3739508/1‑94 (MQ=60)

                 AGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTC                                                                              >  SRR4302533.914652/1‑94 (MQ=60)

                 AGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTC                                                                              >  SRR4302533.5091486/1‑94 (MQ=60)

                 AGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTC                                                                              >  SRR4302533.1149481/1‑94 (MQ=60)

                  GCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCGCGTCTCT                                                                             >  SRR4302533.2218294/1‑94 (MQ=60)

                   CAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTT                                                                            >  SRR4302533.1533050/1‑94 (MQ=60)

                   CAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTT                                                                            >  SRR4302533.5481485/1‑94 (MQ=60)

                   CAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTT                                                                            <  SRR4302533.314581/94‑1 (MQ=60)

                   CAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTT                                                                            <  SRR4302533.5803179/94‑1 (MQ=60)

                    AGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTT                                                                           <  SRR4302533.428313/94‑1 (MQ=60)

                    AGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTT                                                                           >  SRR4302533.3436679/1‑94 (MQ=60)

                       CCGCTTCCCAGCAGCAGCAAAGCCAACGCACAGCAAGCCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACA                                                                        <  SRR4302533.995926/94‑1 (MQ=60)

                       CCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACA                                                                        <  SRR4302533.5280797/94‑1 (MQ=60)

                         GCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAG                                                                      >  SRR4302533.4149725/1‑94 (MQ=60)

                         GCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAG                                                                      <  SRR4302533.3410752/94‑1 (MQ=60)

                         GCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAG                                                                      <  SRR4302533.1790915/94‑1 (MQ=60)

                         GCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAG                                                                      <  SRR4302533.2119696/94‑1 (MQ=60)

                          CTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGG                                                                     <  SRR4302533.1906153/94‑1 (MQ=60)

                          CTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGG                                                                     >  SRR4302533.2193335/1‑94 (MQ=60)

                          CTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGG                                                                     >  SRR4302533.4343217/1‑94 (MQ=60)

                           TTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGG                                                                    >  SRR4302533.5565863/1‑94 (MQ=60)

                            TCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGC                                                                   <  SRR4302533.950346/94‑1 (MQ=60)

                            TCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGC                                                                   <  SRR4302533.3741997/94‑1 (MQ=60)

                            TCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGC                                                                   <  SRR4302533.3959741/94‑1 (MQ=60)

                             CCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCG                                                                  <  SRR4302533.4489335/94‑1 (MQ=60)

                              CCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGT                                                                 >  SRR4302533.2333043/1‑94 (MQ=60)

                               CAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTG                                                                <  SRR4302533.3705088/94‑1 (MQ=60)

                               CAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTG                                                                <  SRR4302533.3111941/94‑1 (MQ=60)

                                AGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGT                                                               <  SRR4302533.5289972/94‑1 (MQ=60)

                                AGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGT                                                               <  SRR4302533.5090326/94‑1 (MQ=60)

                                 GCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTA                                                              <  SRR4302533.5436651/94‑1 (MQ=60)

                                     CAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAG                                                          <  SRR4302533.4746661/94‑1 (MQ=60)

                                     CAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAG                                                          <  SRR4302533.4200326/94‑1 (MQ=60)

                                       GCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGC                                                        <  SRR4302533.5690839/94‑1 (MQ=60)

                                       GCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGC                                                        <  SRR4302533.5849517/94‑1 (MQ=60)

                                       GCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGC                                                        <  SRR4302533.2764091/94‑1 (MQ=60)

                                          AAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAAC                                                     <  SRR4302533.2609056/94‑1 (MQ=60)

                                           AGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACA                                                    <  SRR4302533.1981282/94‑1 (MQ=60)

                                           AGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACA                                                    <  SRR4302533.3962199/94‑1 (MQ=60)

                                           AGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACA                                                    <  SRR4302533.723590/94‑1 (MQ=60)

                                           AGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACA                                                    <  SRR4302533.5454182/94‑1 (MQ=60)

                                             CCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGC                                                  <  SRR4302533.4872235/94‑1 (MQ=60)

                                              CAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCG                                                 <  SRR4302533.4686842/94‑1 (MQ=60)

                                               AACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGG                                                <  SRR4302533.1369264/94‑1 (MQ=60)

                                               AACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGGGTAATAGGCAACAGCGG                                                >  SRR4302533.2486288/1‑94 (MQ=60)

                                                 CGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACCAGGGCGTGTAACAGGCAACAGCGGCG                                              >  SRR4302533.3824510/1‑94 (MQ=60)

                                                  GCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGT                                             >  SRR4302533.5119403/1‑94 (MQ=60)

                                                     CAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGA                                          <  SRR4302533.3991129/94‑1 (MQ=60)

                                                      AGCAAACCATGATCCTCTGGCGGGGGAAGACGACGATACACTGCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGAT                                         <  SRR4302533.4378844/94‑1 (MQ=60)

                                                       GCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATA                                        <  SRR4302533.4518979/94‑1 (MQ=60)

                                                        CAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAT                                       <  SRR4302533.4786945/94‑1 (MQ=60)

                                                         AAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATT                                      >  SRR4302533.4062209/1‑94 (MQ=60)

                                                         AAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATT                                      >  SRR4302533.1081870/1‑94 (MQ=60)

                                                         AAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATT                                      >  SRR4302533.2178154/1‑94 (MQ=60)

                                                         AAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATT                                      >  SRR4302533.1584132/1‑94 (MQ=60)

                                                          AACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTATCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTT                                     >  SRR4302533.3635891/1‑94 (MQ=60)

                                                           ACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTT                                    <  SRR4302533.5892039/94‑1 (MQ=60)

                                                           ACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTT                                    >  SRR4302533.5683936/1‑94 (MQ=60)

                                                            CCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTC                                   <  SRR4302533.6014887/94‑1 (MQ=60)

                                                            CCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTC                                   >  SRR4302533.2017782/1‑94 (MQ=60)

                                                             CATGAGCCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCG                                  <  SRR4302533.3044311/94‑1 (MQ=60)

                                                             CATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCG                                  <  SRR4302533.1413770/94‑1 (MQ=60)

                                                               TGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCC                                <  SRR4302533.5223999/94‑1 (MQ=60)

                                                                GAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCT                               >  SRR4302533.5533770/1‑94 (MQ=60)

                                                                 AACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTA                              >  SRR4302533.4589320/1‑94 (MQ=60)

                                                                  ACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAA                             >  SRR4302533.2293729/1‑94 (MQ=60)

                                                                  ACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAA                             >  SRR4302533.3727129/1‑94 (MQ=60)

                                                                    CTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACG                           <  SRR4302533.6081687/94‑1 (MQ=60)

                                                                    CTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACG                           <  SRR4302533.2421812/94‑1 (MQ=60)

                                                                    CTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACG                           <  SRR4302533.1439308/94‑1 (MQ=60)

                                                                    CTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACG                           <  SRR4302533.5091300/94‑1 (MQ=60)

                                                                     CCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGT                          <  SRR4302533.17467/94‑1 (MQ=60)

                                                                         GCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGA                      <  SRR4302533.2591199/94‑1 (MQ=60)

                                                                         GCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGA                      <  SRR4302533.5956973/94‑1 (MQ=60)

                                                                          CGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAG                     <  SRR4302533.1706808/94‑1 (MQ=60)

                                                                          CGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAG                     >  SRR4302533.2675788/1‑94 (MQ=60)

                                                                           GGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGG                    >  SRR4302533.1085784/1‑94 (MQ=60)

                                                                            GGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGT                   <  SRR4302533.3240578/94‑1 (MQ=60)

                                                                            GGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGT                   <  SRR4302533.2659537/94‑1 (MQ=60)

                                                                             GGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTA                  <  SRR4302533.6131187/94‑1 (MQ=60)

                                                                              GGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAG                 <  SRR4302533.5970219/94‑1 (MQ=60)

                                                                               GAGGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGC                <  SRR4302533.1912345/94‑1 (MQ=60)

                                                                                AAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCA               <  SRR4302533.3212514/94‑1 (MQ=60)

                                                                                  GACGACGATACACTTCCGGTTCTCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACC             <  SRR4302533.5691162/94‑1 (MQ=60)

                                                                                  GACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACC             <  SRR4302533.441716/94‑1 (MQ=60)

                                                                                  GACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACC             <  SRR4302533.6175121/94‑1 (MQ=60)

                                                                                   ACGACGATACACTGCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCG            >  SRR4302533.3426550/1‑94 (MQ=60)

                                                                                    CGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGT           <  SRR4302533.1640515/94‑1 (MQ=60)

                                                                                     GACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTA          >  SRR4302533.680186/1‑94 (MQ=60)

                                                                                       CGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAAT        <  SRR4302533.2111454/94‑1 (MQ=60)

                                                                                        GATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATC       <  SRR4302533.2816028/94‑1 (MQ=60)

                                                                                         ATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCC      <  SRR4302533.6214470/94‑1 (MQ=60)

                                                                                         ATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCC      <  SRR4302533.668542/94‑1 (MQ=60)

                                                                                          TACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCG     >  SRR4302533.955193/1‑94 (MQ=60)

                                                                                           ACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGC    <  SRR4302533.1824223/94‑1 (MQ=60)

                                                                                            CACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCG   <  SRR4302533.2317811/94‑1 (MQ=60)

                                                                                             ACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGT  <  SRR4302533.1245064/94‑1 (MQ=60)

AAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGT  >  NC_000913/2017376‑2017562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`