Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,043,272	A→G	S130G (AGT→GGT)	b1978 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,043,272	0	A	G	100.0%	925.7 / NA	286	S130G (AGT→GGT)	b1978	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (118/168); total (118/168)

Only 100 of 289 total aligned reads displayed.

GCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAAGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGT  >  NC_000913/2043182‑2043361

gCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGt                                                                                         >  2:6264106/1‑93 (MQ=255)

  gTTGGAATCGGCCCAAAGCGTTGCCGAAGGTTTCGGTATTTCGGTGGCTGAGTTACGCTAATTCAAACATGTTTGTACGTTTGCTCGTGGttt                                                                                       >  2:4108603/1‑93 (MQ=255)

   ttGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGtttt                                                                                      <  1:9371610/93‑1 (MQ=255)

    tGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTg                                                                                     <  2:3521919/93‑1 (MQ=255)

      gAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGAt                                                                                   <  1:9857218/93‑1 (MQ=255)

       aaTCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATa                                                                                  <  2:2886934/93‑1 (MQ=255)

       aaTCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATa                                                                                  <  1:7066712/93‑1 (MQ=255)

        aTCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATaa                                                                                 <  1:8990997/93‑1 (MQ=255)

         tCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAAt                                                                                <  1:6365427/93‑1 (MQ=255)

          cGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATg                                                                               <  2:4683289/93‑1 (MQ=255)

          cGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATg                                                                               <  1:2499957/93‑1 (MQ=255)

           ggCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGt                                                                              <  2:3106798/93‑1 (MQ=255)

            gCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTc                                                                             <  2:4195699/93‑1 (MQ=255)

            gCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTc                                                                             <  1:1499558/93‑1 (MQ=255)

             cccAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTcc                                                                            <  2:6871352/93‑1 (MQ=255)

               cAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGc                                                                          >  2:4570881/1‑93 (MQ=255)

               cAAAGCGTTGCCCAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGGGGTTTTGATAATGTCCGc                                                                          <  2:5332128/93‑1 (MQ=255)

                aaaGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGcc                                                                         <  2:2160491/93‑1 (MQ=255)

                aaaGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGcc                                                                         >  1:6467388/1‑93 (MQ=255)

                   gCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAgg                                                                      <  1:3949084/93‑1 (MQ=255)

                   gCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAgg                                                                      >  2:3185313/1‑93 (MQ=255)

                    cGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAggg                                                                     >  1:5621561/1‑93 (MQ=255)

                      ttGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGTTCGAGGTTTTGATAATGTCCGCCAgggcg                                                                   >  1:5837793/1‑91 (MQ=255)

                        gCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGtgat                                                                 <  2:5507759/93‑1 (MQ=255)

                         ccGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGtgatg                                                                <  2:6436905/93‑1 (MQ=255)

                         ccGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGtgatg                                                                <  2:3719570/93‑1 (MQ=255)

                           gAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGaa                                                              >  1:8354187/1‑93 (MQ=255)

                            aaCGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAAc                                                             <  1:3259538/93‑1 (MQ=255)

                            aaCGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAAc                                                             <  2:636116/93‑1 (MQ=255)

                              cGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTg                                                           <  1:5728702/93‑1 (MQ=255)

                              cGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTg                                                           >  2:6645590/1‑93 (MQ=255)

                              cGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTg                                                           <  1:8557769/93‑1 (MQ=255)

                               gTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTgg                                                          >  2:4937047/1‑93 (MQ=255)

                                  tCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATg                                                       >  1:3615239/1‑93 (MQ=255)

                                  tCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATg                                                       >  1:4953754/1‑93 (MQ=255)

                                   cGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGt                                                      >  1:1008218/1‑93 (MQ=255)

                                     gTATTTCGGTGTCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTcc                                                    <  2:6138040/93‑1 (MQ=255)

                                     gTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTcc                                                    <  2:8111169/93‑1 (MQ=255)

                                     gTATTTCGATGGCTGACTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTcc                                                    <  2:7790723/93‑1 (MQ=255)

                                      tATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTccc                                                   <  2:1111416/93‑1 (MQ=255)

                                      tATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTccc                                                   >  1:951600/1‑93 (MQ=255)

                                         ttCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGc                                                <  1:9974131/93‑1 (MQ=255)

                                         ttCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGc                                                <  1:7508095/93‑1 (MQ=255)

                                           cGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGcac                                              <  2:9095024/93‑1 (MQ=255)

                                            ggTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGcaca                                             <  2:7211691/93‑1 (MQ=255)

                                            ggTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGcaca                                             >  2:5734911/1‑93 (MQ=255)

                                             gTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACaa                                            <  1:921456/93‑1 (MQ=255)

                                             gTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACaa                                            <  1:8102428/93‑1 (MQ=255)

                                             gTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACaa                                            <  2:2535667/93‑1 (MQ=255)

                                               ggCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTCGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGt                                          <  1:7353838/93‑1 (MQ=255)

                                                gCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGtt                                         <  1:8177972/93‑1 (MQ=255)

                                                 cTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTa                                        <  2:9422621/93‑1 (MQ=255)

                                                 cTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTa                                        <  1:4676760/93‑1 (MQ=255)

                                                  tGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAg                                       >  1:3707022/1‑93 (MQ=255)

                                                  tGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAg                                       <  1:4506641/93‑1 (MQ=255)

                                                  tGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAg                                       >  2:3139341/1‑93 (MQ=255)

                                                   gAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGt                                      <  1:2793858/93‑1 (MQ=255)

                                                     gTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGa                                    <  1:2305197/93‑1 (MQ=255)

                                                       tACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGaaa                                  >  2:328603/1‑93 (MQ=255)

                                                         cGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGaaaaa                                <  1:9620430/93‑1 (MQ=255)

                                                          gCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGaaaaaa                               <  2:8461896/93‑1 (MQ=255)

                                                          gCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTGTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGagaaaa                               >  1:6422847/1‑93 (MQ=255)

                                                            aaaCTCACCCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGaaaaaaaa                             <  1:610567/93‑1 (MQ=255)

                                                            aaaCTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGaaaaaaaa                             <  1:3124841/93‑1 (MQ=255)

                                                             aaCTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAAt                            >  2:7471986/1‑93 (MQ=255)

                                                             aaCTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAAt                            <  2:509164/93‑1 (MQ=255)

                                                                tCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTaa                         <  2:7425069/93‑1 (MQ=255)

                                                                tCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTaa                         >  1:1825873/1‑93 (MQ=255)

                                                                tCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGGCCCGGCACAAGTTAGTGAAAAAAAATTaa                         >  1:2174857/1‑93 (MQ=255)

                                                                 cAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCTCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAc                        <  2:3998745/93‑1 (MQ=255)

                                                                  aaCCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAcc                       <  1:7290159/93‑1 (MQ=255)

                                                                   aCCAGTTTCGTACGTTTGCTCGAGGTTTTGATATTGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAccc                      <  1:9702133/93‑1 (MQ=255)

                                                                   aCCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAccc                      >  1:6202237/1‑93 (MQ=255)

                                                                   aCCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAccc                      <  1:4387214/93‑1 (MQ=255)

                                                                   aCCAGTATCGTACGTTTGCTCGAGATTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAccc                      <  2:9242191/93‑1 (MQ=255)

                                                                    ccAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAcccc                     <  2:1446284/93‑1 (MQ=255)

                                                                       gTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCccgcc                  <  2:1711839/93‑1 (MQ=255)

                                                                       gTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCccgcc                  <  1:9262758/93‑1 (MQ=255)

                                                                         ttCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCccgccgc                >  1:2056143/1‑93 (MQ=255)

                                                                          tCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCccgccgcc               <  2:210906/93‑1 (MQ=255)

                                                                           cGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCccgccgccg              <  2:9769866/93‑1 (MQ=255)

                                                                            gTACGTTTGCTCGAGGTTTTGATAATGTCCGCCCGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACACCGCCGCCgg             >  1:6622355/1‑93 (MQ=255)

                                                                            gTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCgg             <  2:4792936/93‑1 (MQ=255)

                                                                             tACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCggg            >  1:5403083/1‑93 (MQ=255)

                                                                             tACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCggg            >  2:885602/1‑93 (MQ=255)

                                                                             tACGTTTGCTCGAGGTTTTGATAATGCCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCggg            >  2:7823548/1‑93 (MQ=255)

                                                                              aCGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCggga           >  2:7055883/1‑92 (MQ=255)

                                                                              aCGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCggga           >  1:6240337/1‑92 (MQ=255)

                                                                               cGTTTGCTGGAGGTTTTGATAGTGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTa          <  2:5921258/93‑1 (MQ=255)

                                                                               cGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTa          >  1:5140646/1‑93 (MQ=255)

                                                                               cGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTa          <  1:7743193/93‑1 (MQ=255)

                                                                                 tttGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAAt        <  2:5780186/93‑1 (MQ=255)

                                                                                 tttGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAAt        >  2:9537386/1‑93 (MQ=255)

                                                                                 tttGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAAt        <  2:3813274/93‑1 (MQ=255)

                                                                                 tttGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAAt        <  2:6905838/93‑1 (MQ=255)

                                                                                 tttGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAAt        >  2:4019982/1‑93 (MQ=255)

                                                                                  ttGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATa       <  2:2632627/93‑1 (MQ=255)

                                                                                   tGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAg      <  2:3406462/93‑1 (MQ=255)

                                                                                   tGCTCGAGGTGTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAg      <  1:1790572/93‑1 (MQ=255)

                                                                                     cTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCa    <  1:1195250/93‑1 (MQ=255)

GCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAAGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGT  >  NC_000913/2043182‑2043361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAAGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACA  >  NC_000913/2043179‑2043365

GGAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAG                                                                                               <  SRR4302540.7742069/94‑1 (MQ=60)

 GAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGG                                                                                              >  SRR4302540.9315385/1‑94 (MQ=60)

  AGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGT                                                                                             >  SRR4302540.1319304/1‑94 (MQ=60)

     GTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTT                                                                                          <  SRR4302540.2036723/94‑1 (MQ=60)

     GTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTT                                                                                          <  SRR4302540.6369243/94‑1 (MQ=60)

     GTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTT                                                                                          >  SRR4302540.738712/1‑94 (MQ=60)

      TTGGCATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTG                                                                                         <  SRR4302540.6491843/94‑1 (MQ=60)

      TTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTG                                                                                         <  SRR4302540.9483188/94‑1 (MQ=60)

      TTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGATTTG                                                                                         >  SRR4302540.6192406/1‑94 (MQ=60)

       TGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGA                                                                                        >  SRR4302540.4407428/1‑94 (MQ=60)

       TGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGA                                                                                        >  SRR4302540.5789299/1‑94 (MQ=60)

         GAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATA                                                                                      <  SRR4302540.9666545/94‑1 (MQ=60)

         GAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATA                                                                                      <  SRR4302540.9972406/94‑1 (MQ=60)

          AATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAA                                                                                     <  SRR4302540.7145307/94‑1 (MQ=60)

          AATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAA                                                                                     <  SRR4302540.7603946/94‑1 (MQ=60)

           ATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAAT                                                                                    >  SRR4302540.7288733/1‑94 (MQ=60)

           ATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAAT                                                                                    <  SRR4302540.9098682/94‑1 (MQ=60)

           ATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAAT                                                                                    >  SRR4302540.5572536/1‑94 (MQ=60)

           ATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAAT                                                                                    <  SRR4302540.10087172/94‑1 (MQ=60)

            TCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATG                                                                                   <  SRR4302540.6436301/94‑1 (MQ=60)

             CGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGT                                                                                  >  SRR4302540.3696624/1‑94 (MQ=60)

             CGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGT                                                                                  >  SRR4302540.36587/1‑94 (MQ=60)

             CGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGT                                                                                  <  SRR4302540.7081135/94‑1 (MQ=60)

             CGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGT                                                                                  <  SRR4302540.2526924/94‑1 (MQ=60)

               GCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCC                                                                                <  SRR4302540.5934764/94‑1 (MQ=60)

               GCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCC                                                                                <  SRR4302540.6248794/94‑1 (MQ=60)

               GCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCC                                                                                >  SRR4302540.7752413/1‑94 (MQ=60)

               GCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCC                                                                                <  SRR4302540.1516080/94‑1 (MQ=60)

                CCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCG                                                                               <  SRR4302540.959613/94‑1 (MQ=60)

                  CAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCC                                                                             >  SRR4302540.6539351/1‑94 (MQ=60)

                   AAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCA                                                                            <  SRR4302540.1618377/94‑1 (MQ=60)

                     AGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGG                                                                          <  SRR4302540.6348037/94‑1 (MQ=60)

                      GCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGG                                                                         >  SRR4302540.5683412/1‑94 (MQ=60)

                      GCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGG                                                                         <  SRR4302540.3991723/94‑1 (MQ=60)

                       CGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGT                                                                        >  SRR4302540.1830043/1‑94 (MQ=60)

                        GTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGTTCGAGGTTTTGATAATGTCCGCCAGGGCG                                                                       >  SRR4302540.5902344/1‑94 (MQ=60)

                        GTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTG                                                                       <  SRR4302540.1608671/94‑1 (MQ=60)

                        GTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTG                                                                       >  SRR4302540.5902343/1‑94 (MQ=60)

                        GTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTG                                                                       >  SRR4302540.1271874/1‑94 (MQ=60)

                         TTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGA                                                                      <  SRR4302540.9336721/94‑1 (MQ=60)

                            GCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGA                                                                   <  SRR4302540.2422522/94‑1 (MQ=60)

                             CGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAA                                                                  >  SRR4302540.8451150/1‑94 (MQ=60)

                             CGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAA                                                                  >  SRR4302540.8451151/1‑94 (MQ=60)

                             CGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAA                                                                  >  SRR4302540.2401924/1‑94 (MQ=60)

                             CGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAA                                                                  >  SRR4302540.5137301/1‑94 (MQ=60)

                             CGAACGTTTCGGTATTTCGGTGGCTGAGTGACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAA                                                                  <  SRR4302540.6503041/94‑1 (MQ=60)

                              GAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAAC                                                                 >  SRR4302540.10054216/1‑94 (MQ=60)

                              GAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAAC                                                                 <  SRR4302540.4990289/94‑1 (MQ=60)

                              GAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAAC                                                                 >  SRR4302540.4497524/1‑94 (MQ=60)

                               AACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACT                                                                <  SRR4302540.3295085/94‑1 (MQ=60)

                               AACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACT                                                                <  SRR4302540.3295086/94‑1 (MQ=60)

                                ACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTG                                                               <  SRR4302540.6686798/94‑1 (MQ=60)

                                 CGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGG                                                              >  SRR4302540.2088479/1‑94 (MQ=60)

                                 CGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGG                                                              <  SRR4302540.8657169/94‑1 (MQ=60)

                                 CGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGG                                                              <  SRR4302540.5791788/94‑1 (MQ=60)

                                  GTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGA                                                             >  SRR4302540.7539518/1‑94 (MQ=60)

                                  GTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGA                                                             >  SRR4302540.5605973/1‑94 (MQ=60)

                                  GTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGA                                                             <  SRR4302540.9304675/94‑1 (MQ=60)

                                  GTTTCGGTAATTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGCGATGAACTGGA                                                             >  SRR4302540.7539517/1‑94 (MQ=60)

                                   TTTCGGTATTTCGGTGGCTGAGTTACGCAAGCTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGAT                                                            <  SRR4302540.7050891/94‑1 (MQ=60)

                                   TTTCGGTATTTCGGTGGCTAAGTTCCGCCAACACAACCAGTTTCGTACGATTGCTCGAGGTTTTGATAATGTCCGCCAGGGTAATGAGCTGGGT                                                            <  SRR4302540.9047567/94‑1 (MQ=60)

                                    TTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATG                                                           >  SRR4302540.5007279/1‑94 (MQ=60)

                                    TTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATG                                                           >  SRR4302540.3654773/1‑94 (MQ=60)

                                    TTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATG                                                           >  SRR4302540.1135531/1‑94 (MQ=60)

                                     TCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGT                                                          >  SRR4302540.1018447/1‑94 (MQ=60)

                                       GGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCC                                                        >  SRR4302540.2611939/1‑94 (MQ=60)

                                        GTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCC                                                       <  SRR4302540.8439408/94‑1 (MQ=60)

                                        GTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCC                                                       <  SRR4302540.3184540/94‑1 (MQ=60)

                                        GTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCC                                                       >  SRR4302540.961487/1‑94 (MQ=60)

                                          ATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGG                                                     <  SRR4302540.5302481/94‑1 (MQ=60)

                                          ATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGG                                                     >  SRR4302540.2116419/1‑94 (MQ=60)

                                          ATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGG                                                     >  SRR4302540.9648965/1‑94 (MQ=60)

                                            TTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCA                                                   <  SRR4302540.7592012/94‑1 (MQ=60)

                                            TTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCA                                                   <  SRR4302540.10090465/94‑1 (MQ=60)

                                                GTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAG                                               <  SRR4302540.931176/94‑1 (MQ=60)

                                                GTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAG                                               >  SRR4302540.9940504/1‑94 (MQ=60)

                                                GTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAG                                               <  SRR4302540.8196466/94‑1 (MQ=60)

                                                GTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAG                                               >  SRR4302540.2821623/1‑94 (MQ=60)

                                                GTGGCTGAGTTACGCAAAATCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGATGATGAACTGGATGTCCCGGCACAAG                                               <  SRR4302540.3366592/94‑1 (MQ=60)

                                                 TGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGT                                              >  SRR4302540.5881810/1‑94 (MQ=60)

                                                 TGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGT                                              >  SRR4302540.4589927/1‑94 (MQ=60)

                                                 TGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGT                                              <  SRR4302540.9809143/94‑1 (MQ=60)

                                                 TGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGT                                              >  SRR4302540.8357616/1‑94 (MQ=60)

                                                  GGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTT                                             <  SRR4302540.722146/94‑1 (MQ=60)

                                                  GGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTCGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTT                                             <  SRR4302540.7435859/94‑1 (MQ=60)

                                                   GCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTA                                            <  SRR4302540.8272964/94‑1 (MQ=60)

                                                    CTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAG                                           <  SRR4302540.4726799/94‑1 (MQ=60)

                                                    CTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAG                                           >  SRR4302540.3747481/1‑94 (MQ=60)

                                                     TGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGT                                          <  SRR4302540.4555485/94‑1 (MQ=60)

                                                      GAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTG                                         <  SRR4302540.4453512/94‑1 (MQ=60)

                                                      GAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTG                                         <  SRR4302540.2824022/94‑1 (MQ=60)

                                                      GAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTG                                         <  SRR4302540.2824023/94‑1 (MQ=60)

                                                        GTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAA                                       <  SRR4302540.6517958/94‑1 (MQ=60)

                                                        GTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAA                                       <  SRR4302540.2329999/94‑1 (MQ=60)

                                                         TTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAA                                      <  SRR4302540.3950563/94‑1 (MQ=60)

                                                          TACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAA                                     >  SRR4302540.7898858/1‑94 (MQ=60)

                                                          TACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAA                                     >  SRR4302540.8927686/1‑94 (MQ=60)

                                                            CGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAA                                   <  SRR4302540.9733829/94‑1 (MQ=60)

                                                            CGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAA                                   >  SRR4302540.9110394/1‑94 (MQ=60)

                                                            CGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTGTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAGAAAA                                   >  SRR4302540.6494339/1‑94 (MQ=60)

                                                             GCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAACAAA                                  >  SRR4302540.4876709/1‑94 (MQ=60)

                                                             GCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAA                                  >  SRR4302540.4876710/1‑94 (MQ=60)

                                                               AAACTCACCCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAAT                                <  SRR4302540.617091/94‑1 (MQ=60)

                                                               AAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAAT                                <  SRR4302540.7374087/94‑1 (MQ=60)

                                                               AAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAAT                                <  SRR4302540.3158968/94‑1 (MQ=60)

                                                                AACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATT                               >  SRR4302540.9623209/1‑94 (MQ=60)

                                                                 ACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTA                              <  SRR4302540.9832877/94‑1 (MQ=60)

                                                                  CTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAA                             >  SRR4302540.1845840/1‑94 (MQ=60)

                                                                  CTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAA                             <  SRR4302540.9440353/94‑1 (MQ=60)

                                                                  CTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGGCCCGGCACAAGTTAGTGAAAAAAAATTAA                             >  SRR4302540.2198312/1‑94 (MQ=60)

                                                                   TCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAC                            >  SRR4302540.6643198/1‑94 (MQ=60)

                                                                   TCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAC                            <  SRR4302540.7932756/94‑1 (MQ=60)

                                                                    CAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACC                           >  SRR4302540.6304721/1‑94 (MQ=60)

                                                                     AACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCC                          >  SRR4302540.6271274/1‑94 (MQ=60)

                                                                     AACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCC                          <  SRR4302540.6304709/94‑1 (MQ=60)

                                                                     AACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCC                          <  SRR4302540.7371510/94‑1 (MQ=60)

                                                                      ACCAGTTTCGTACGTTTGCTCGAGGTTTTGATATTGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCC                         <  SRR4302540.9816189/94‑1 (MQ=60)

                                                                      ACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCC                         <  SRR4302540.4434837/94‑1 (MQ=60)

                                                                          GTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCG                     <  SRR4302540.9373560/94‑1 (MQ=60)

                                                                          GTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCG                     >  SRR4302540.8651129/1‑94 (MQ=60)

                                                                           TTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGC                    >  SRR4302540.2078277/1‑94 (MQ=60)

                                                                             TCGTGCGTTTGCGCGAGATTTTGAGAATGTCCGCGAGGGTGATGAGCTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCG                  <  SRR4302540.861787/94‑1 (MQ=60)

                                                                              CGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCCGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACACCGCCGCCGG                 >  SRR4302540.6695678/1‑94 (MQ=60)

                                                                               GTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGG                >  SRR4302540.5462485/1‑94 (MQ=60)

                                                                               GTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGG                >  SRR4302540.4191165/1‑94 (MQ=60)

                                                                               GTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGG                >  SRR4302540.5960156/1‑94 (MQ=60)

                                                                                TACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGA               >  SRR4302540.6309778/1‑94 (MQ=60)

                                                                                 ACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTA              >  SRR4302540.5196331/1‑94 (MQ=60)

                                                                                 ACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTA              <  SRR4302540.2271255/94‑1 (MQ=60)

                                                                                 ACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGGA              >  SRR4302540.9325421/1‑94 (MQ=60)

                                                                                  CGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAA             >  SRR4302540.186986/1‑94 (MQ=60)

                                                                                  CGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAA             <  SRR4302540.7833598/94‑1 (MQ=60)

                                                                                    TTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGACCTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATA           <  SRR4302540.9401694/94‑1 (MQ=60)

                                                                                      TGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGC         <  SRR4302540.4629218/94‑1 (MQ=60)

                                                                                      TGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGC         <  SRR4302540.1810307/94‑1 (MQ=60)

                                                                                      TGCTCGAGGTGTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGC         <  SRR4302540.1810034/94‑1 (MQ=60)

                                                                                       GCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCA        <  SRR4302540.5747951/94‑1 (MQ=60)

                                                                                       GCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCA        <  SRR4302540.5291853/94‑1 (MQ=60)

                                                                                       GCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCA        >  SRR4302540.3927363/1‑94 (MQ=60)

                                                                                        CTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAG       <  SRR4302540.1207558/94‑1 (MQ=60)

                                                                                         TCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGT      <  SRR4302540.3343797/94‑1 (MQ=60)

                                                                                         TCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGT      <  SRR4302540.2270331/94‑1 (MQ=60)

                                                                                         TCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGG      >  SRR4302540.8240696/1‑94 (MQ=60)

                                                                                          CGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGCG     >  SRR4302540.9594299/1‑94 (MQ=60)

                                                                                            AGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGAC   <  SRR4302540.8040325/94‑1 (MQ=60)

                                                                                             GGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACA  <  SRR4302540.1397124/94‑1 (MQ=60)

                                                                                             GGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACA  >  SRR4302540.7389065/1‑94 (MQ=60)

                                                                                             GGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACA  >  SRR4302540.789520/1‑94 (MQ=60)

GGAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAAGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACA  >  NC_000913/2043179‑2043365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`