Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,043,272	A→G	S130G (AGT→GGT)	b1978 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,043,272	0	A	G	100.0%	661.5 / NA	205	S130G (AGT→GGT)	b1978	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (88/117); total (88/117)

Only 100 of 212 total aligned reads displayed.

GCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAAGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGT  >  NC_000913/2043182‑2043361

gCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGt                                                                                         >  1:520445/1‑93 (MQ=255)

  gTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGttt                                                                                       >  2:5676908/1‑93 (MQ=255)

  gTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGttt                                                                                       <  1:5960720/93‑1 (MQ=255)

   ttGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGtttt                                                                                      <  1:5338213/93‑1 (MQ=255)

   ttGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGtttt                                                                                      >  2:1637677/1‑93 (MQ=255)

     ggAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGa                                                                                    <  2:4187934/93‑1 (MQ=255)

      gAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGAt                                                                                   >  2:1979467/1‑93 (MQ=255)

      gAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGATACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGAt                                                                                   >  1:399797/1‑93 (MQ=255)

       aaTCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATa                                                                                  <  2:5583927/93‑1 (MQ=255)

        aTCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATaa                                                                                 <  1:644849/93‑1 (MQ=255)

        aTCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATaa                                                                                 <  2:2959915/93‑1 (MQ=255)

         tCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAAt                                                                                >  1:1146947/1‑93 (MQ=255)

           ggCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGt                                                                              <  1:2826742/93‑1 (MQ=255)

            gCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTc                                                                             <  1:3709673/93‑1 (MQ=255)

             cccAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTcc                                                                            >  2:5975056/1‑93 (MQ=255)

              ccAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCg                                                                           >  1:2874445/1‑93 (MQ=255)

               cAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGc                                                                          >  2:1147851/1‑93 (MQ=255)

                aaaGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGcc                                                                         >  2:2519513/1‑93 (MQ=255)

                 aaGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCa                                                                        >  2:693772/1‑93 (MQ=255)

                 aaGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCa                                                                        <  1:2449068/93‑1 (MQ=255)

                  aGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAg                                                                       <  1:2324549/93‑1 (MQ=255)

                   gCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAgg                                                                      >  1:6168511/1‑93 (MQ=255)

                   gCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAgg                                                                      <  1:4485892/93‑1 (MQ=255)

                         ccGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGtgatg                                                                >  1:3793494/1‑93 (MQ=255)

                         ccGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGtgatg                                                                <  2:3211878/93‑1 (MQ=255)

                            aaCGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAAc                                                             >  2:519595/1‑93 (MQ=255)

                            aaCGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAAc                                                             >  2:626261/1‑93 (MQ=255)

                             aCGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACt                                                            >  1:4353373/1‑93 (MQ=255)

                              cGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTg                                                           <  1:5610196/93‑1 (MQ=255)

                               gTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTgg                                                          >  2:4294545/1‑93 (MQ=255)

                               gTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTgg                                                          <  2:161263/93‑1 (MQ=255)

                                 ttCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGAt                                                        >  2:4950077/1‑93 (MQ=255)

                                  tCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGAtt                                                       <  1:299107/93‑2 (MQ=255)

                                     gTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTcc                                                    <  2:2846569/93‑1 (MQ=255)

                                      tATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTccc                                                   <  1:1314862/93‑1 (MQ=255)

                                      tATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTccc                                                   >  2:3625098/1‑93 (MQ=255)

                                        tttCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCgg                                                 >  2:1416821/1‑93 (MQ=255)

                                        tttCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCgg                                                 <  2:4119855/93‑1 (MQ=255)

                                        tttCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCgg                                                 >  1:2151317/1‑93 (MQ=255)

                                          tCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGca                                               <  1:2553764/93‑1 (MQ=255)

                                             gTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACaa                                            <  2:1280796/93‑1 (MQ=255)

                                             gTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACaa                                            <  2:3654205/93‑1 (MQ=255)

                                              tGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAg                                           <  1:4269684/93‑1 (MQ=255)

                                               ggCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGt                                          >  2:3366602/1‑93 (MQ=255)

                                                  tGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAg                                       <  1:5254665/93‑1 (MQ=255)

                                                  tGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAg                                       >  2:4456353/1‑93 (MQ=255)

                                                  tGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAg                                       >  2:5427974/1‑93 (MQ=255)

                                                   gAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGt                                      <  2:2161479/93‑1 (MQ=255)

                                                   gAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGt                                      >  2:3469857/1‑93 (MQ=255)

                                                    aGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTg                                     <  1:4050785/93‑1 (MQ=255)

                                                    aGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTg                                     >  1:3483121/1‑93 (MQ=255)

                                                     gTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGa                                    <  2:5307553/93‑1 (MQ=255)

                                                        aCGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGaaaa                                 <  2:1560585/93‑1 (MQ=255)

                                                         cGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGaaaaa                                >  1:1206303/1‑93 (MQ=255)

                                                         cGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGaaaaa                                <  1:499075/93‑1 (MQ=255)

                                                          gCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGaaaaaa                               >  1:4584162/1‑93 (MQ=255)

                                                          gCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGaaaaaa                               >  1:4405039/1‑93 (MQ=255)

                                                          gCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGaaaaaa                               <  1:1979467/93‑1 (MQ=255)

                                                           cAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGaaaaaaa                              >  1:3594862/1‑93 (MQ=255)

                                                            aaaCTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGaaaaaaaa                             >  2:3424326/1‑93 (MQ=255)

                                                             aaCTCAACCAGTTTCGTATGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAAt                            <  1:1346019/93‑1 (MQ=255)

                                                             aaCTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCAGAAGTTAGTGAAAAAAAAt                            >  2:5122713/1‑93 (MQ=255)

                                                             aaCTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAAt                            <  1:1276445/93‑1 (MQ=255)

                                                             aaCTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAAt                            <  2:3809720/93‑1 (MQ=255)

                                                               cTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTa                          <  1:102548/93‑1 (MQ=255)

                                                               cTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTa                          <  1:759150/93‑1 (MQ=255)

                                                               cTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTa                          <  1:1706693/93‑1 (MQ=255)

                                                                tCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGACCCGGCACAAGTTAGTGAAAAAAAATTaa                         <  2:6207024/93‑1 (MQ=255)

                                                                 cAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAc                        <  2:3866487/93‑1 (MQ=255)

                                                                 cAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAc                        <  2:5375508/93‑1 (MQ=255)

                                                                  aaCCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTaaac                       >  2:5491780/1‑91 (MQ=255)

                                                                  aaCCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAcc                       >  1:2697222/1‑93 (MQ=255)

                                                                  aaCCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAcc                       <  1:3423727/93‑1 (MQ=255)

                                                                  aaCCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAcc                       <  1:3117484/93‑1 (MQ=255)

                                                                   aCCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAccc                      >  1:598249/1‑93 (MQ=255)

                                                                   aCCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAccc                      <  2:4528611/93‑1 (MQ=255)

                                                                   aCCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAcac                      <  2:3538550/93‑3 (MQ=255)

                                                                    ccAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAcccc                     <  1:4095325/93‑1 (MQ=255)

                                                                    ccAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAcccc                     >  2:1736367/1‑93 (MQ=255)

                                                                    ccAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAcccc                     <  2:6029211/93‑1 (MQ=255)

                                                                     cAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCccg                    >  2:2969998/1‑93 (MQ=255)

                                                                     cAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCccg                    >  2:2270798/1‑93 (MQ=255)

                                                                      aGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCccgc                   >  1:484181/1‑93 (MQ=255)

                                                                      aGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCccgc                   >  1:3016397/1‑93 (MQ=255)

                                                                       gTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCccgcc                  >  2:1101853/1‑93 (MQ=255)

                                                                        tttCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCccgccg                 >  2:4689063/1‑93 (MQ=255)

                                                                           cGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCccgccgccg              >  2:2535801/1‑93 (MQ=255)

                                                                            gTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCgg             <  1:165414/93‑1 (MQ=255)

                                                                             tACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCggg            >  2:4160576/1‑93 (MQ=255)

                                                                             tACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCggg            >  2:3707993/1‑93 (MQ=255)

                                                                              aCGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCgggc           >  1:5567771/1‑92 (MQ=255)

                                                                               cGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTa          >  2:560077/1‑93 (MQ=255)

                                                                                 tttGCTCGAGGTTTTGATAATGTCCTCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTact        <  1:6089718/93‑3 (MQ=255)

                                                                                 tttGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAAt        <  2:3464266/93‑1 (MQ=255)

                                                                                 tttGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAAt        <  1:4805700/93‑1 (MQ=255)

                                                                                 tttGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAAt        <  1:2422781/93‑1 (MQ=255)

                                                                                 tttGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAAt        >  1:1055506/1‑93 (MQ=255)

                                                                                   tgttCGAGGTTTTGATAATGTCCGCCGGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAg      <  1:514619/90‑1 (MQ=255)

                                                                                     cTCGAGGTTTTGATAATGTCCGCTAGGGTGATGAACTGGATGTCCAGGCACAAGTTAGTGAAAAAAAATTACCCCCGCCGCCGGGTAATAGCa    <  2:2594602/93‑1 (MQ=255)

                                                                                     cTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCa    >  1:2046306/1‑93 (MQ=255)

GCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAAGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGT  >  NC_000913/2043182‑2043361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAAGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACA  >  NC_000913/2043179‑2043365

GGAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAG                                                                                               <  SRR4302533.4357218/94‑1 (MQ=60)

 GAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGG                                                                                              <  SRR4302533.961634/94‑1 (MQ=60)

 GAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGG                                                                                              <  SRR4302533.4293355/94‑1 (MQ=60)

  AGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGT                                                                                             >  SRR4302533.523533/1‑94 (MQ=60)

    CGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTT                                                                                           >  SRR4302533.5500061/1‑94 (MQ=60)

     GTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTT                                                                                          >  SRR4302533.4539322/1‑94 (MQ=60)

     GTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTT                                                                                          <  SRR4302533.5996780/94‑1 (MQ=60)

      TTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTG                                                                                         <  SRR4302533.5370221/94‑1 (MQ=60)

       TGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGA                                                                                        >  SRR4302533.2308482/1‑94 (MQ=60)

        GGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGAT                                                                                       >  SRR4302533.4481908/1‑94 (MQ=60)

        GGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGATACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGAT                                                                                       >  SRR4302533.402209/1‑94 (MQ=60)

           ATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAAT                                                                                    <  SRR4302533.4876330/94‑1 (MQ=60)

           ATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAAT                                                                                    <  SRR4302533.648645/94‑1 (MQ=60)

           ATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAAT                                                                                    <  SRR4302533.655737/94‑1 (MQ=60)

           ATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAAT                                                                                    >  SRR4302533.1153793/1‑94 (MQ=60)

            TCGGCGCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCACCCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATG                                                                                   <  SRR4302533.3907718/94‑1 (MQ=60)

              GGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTC                                                                                 <  SRR4302533.2844134/94‑1 (MQ=60)

               GCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGGTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCC                                                                                <  SRR4302533.44206/94‑1 (MQ=60)

               GCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCC                                                                                <  SRR4302533.5449820/94‑1 (MQ=60)

               GCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCC                                                                                <  SRR4302533.3732456/94‑1 (MQ=60)

               GCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCC                                                                                >  SRR4302533.6014618/1‑94 (MQ=60)

               GCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCC                                                                                >  SRR4302533.1539041/1‑94 (MQ=60)

                CCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCG                                                                               >  SRR4302533.2892130/1‑94 (MQ=60)

                CCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCG                                                                               <  SRR4302533.5180630/94‑1 (MQ=60)

                CCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCG                                                                               >  SRR4302533.3746553/1‑94 (MQ=60)

                 CCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGC                                                                              <  SRR4302533.4210364/94‑1 (MQ=60)

                    AAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAG                                                                           <  SRR4302533.2464184/94‑1 (MQ=60)

                     AGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGG                                                                          <  SRR4302533.2338940/94‑1 (MQ=60)

                     AGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGG                                                                          >  SRR4302533.6205592/1‑94 (MQ=60)

                     AGCGTTGCCGAACGTTTCGGTATTTCAGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGG                                                                          <  SRR4302533.1295295/94‑1 (MQ=60)

                      GCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGG                                                                         <  SRR4302533.4513049/94‑1 (MQ=60)

                      GCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGG                                                                         <  SRR4302533.5242874/94‑1 (MQ=60)

                        GTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTG                                                                       <  SRR4302533.2717840/94‑1 (MQ=60)

                           GCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATG                                                                    >  SRR4302533.3816711/1‑94 (MQ=60)

                            CCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGA                                                                   >  SRR4302533.5291834/1‑94 (MQ=60)

                             CGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAA                                                                  >  SRR4302533.8809/1‑94 (MQ=60)

                               AACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACT                                                                <  SRR4302533.2524091/94‑1 (MQ=60)

                               AACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACT                                                                >  SRR4302533.4379649/1‑94 (MQ=60)

                                ACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTG                                                               >  SRR4302533.1345138/1‑94 (MQ=60)

                                ACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTG                                                               <  SRR4302533.1266124/94‑1 (MQ=60)

                                 CGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGG                                                              <  SRR4302533.5644103/94‑1 (MQ=60)

                                   TTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGAT                                                            <  SRR4302533.1091110/94‑1 (MQ=60)

                                   TTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGAT                                                            <  SRR4302533.371038/94‑1 (MQ=60)

                                     TCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATTT                                                          <  SRR4302533.300938/94‑1 (MQ=60)

                                       GGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCC                                                        >  SRR4302533.4096471/1‑94 (MQ=60)

                                        GTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCC                                                       <  SRR4302533.5880791/94‑1 (MQ=60)

                                         TATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCG                                                      <  SRR4302533.1322749/94‑1 (MQ=60)

                                          ATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGG                                                     >  SRR4302533.5610778/1‑94 (MQ=60)

                                          ATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGG                                                     >  SRR4302533.2164565/1‑94 (MQ=60)

                                             TCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCAC                                                  <  SRR4302533.2569476/94‑1 (MQ=60)

                                               GGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAA                                                <  SRR4302533.366265/94‑1 (MQ=60)

                                                GTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAG                                               >  SRR4302533.6057757/1‑94 (MQ=60)

                                                 TGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGT                                              <  SRR4302533.1037094/94‑1 (MQ=60)

                                                 TGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGT                                              <  SRR4302533.4295386/94‑1 (MQ=60)

                                                  GGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTT                                             <  SRR4302533.4642735/94‑1 (MQ=60)

                                                  GGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTT                                             >  SRR4302533.1697995/1‑94 (MQ=60)

                                                   GCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCAGGCACAAGTTA                                            <  SRR4302533.3093162/94‑1 (MQ=60)

                                                    CTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAG                                           >  SRR4302533.3584717/1‑94 (MQ=60)

                                                    CTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAG                                           <  SRR4302533.3017250/94‑1 (MQ=60)

                                                     TGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGT                                          <  SRR4302533.5286232/94‑1 (MQ=60)

                                                     TGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGT                                          <  SRR4302533.2097834/94‑1 (MQ=60)

                                                      GAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTG                                         >  SRR4302533.3504607/1‑94 (MQ=60)

                                                      GAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTG                                         <  SRR4302533.2406613/94‑1 (MQ=60)

                                                       AGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGA                                        <  SRR4302533.4075461/94‑1 (MQ=60)

                                                        GTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAA                                       <  SRR4302533.1765988/94‑1 (MQ=60)

                                                          TACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAA                                     <  SRR4302533.6299005/94‑1 (MQ=60)

                                                           ACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAA                                    >  SRR4302533.1213547/1‑94 (MQ=60)

                                                            CGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAA                                   <  SRR4302533.502046/94‑1 (MQ=60)

                                                            CGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAA                                   <  SRR4302533.5105783/94‑1 (MQ=60)

                                                            CGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAA                                   >  SRR4302533.4431635/1‑94 (MQ=60)

                                                            CGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAA                                   >  SRR4302533.4611933/1‑94 (MQ=60)

                                                             GCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAA                                  >  SRR4302533.3616980/1‑94 (MQ=60)

                                                             GCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAA                                  <  SRR4302533.1991589/94‑1 (MQ=60)

                                                               AAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAAT                                <  SRR4302533.328104/94‑1 (MQ=60)

                                                                AACTCAACCAGTTTCGTATGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATT                               <  SRR4302533.1354119/94‑1 (MQ=60)

                                                                AACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATT                               <  SRR4302533.4840245/94‑1 (MQ=60)

                                                                AACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATT                               <  SRR4302533.1284117/94‑1 (MQ=60)

                                                                  CTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAA                             <  SRR4302533.763618/94‑1 (MQ=60)

                                                                  CTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAA                             <  SRR4302533.1717034/94‑1 (MQ=60)

                                                                  CTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAA                             <  SRR4302533.103186/94‑1 (MQ=60)

                                                                   TCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAC                            <  SRR4302533.4071767/94‑1 (MQ=60)

                                                                    CAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACC                           >  SRR4302533.2713748/1‑94 (MQ=60)

                                                                     AACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCC                          >  SRR4302533.4378227/1‑94 (MQ=60)

                                                                     AACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCC                          >  SRR4302533.601773/1‑94 (MQ=60)

                                                                     AACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCC                          <  SRR4302533.3136650/94‑1 (MQ=60)

                                                                     AACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCC                          <  SRR4302533.3444878/94‑1 (MQ=60)

                                                                       CCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCG                        >  SRR4302533.2738863/1‑94 (MQ=60)

                                                                       CCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCG                        <  SRR4302533.4120257/94‑1 (MQ=60)

                                                                        CAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGC                       >  SRR4302533.487075/1‑94 (MQ=60)

                                                                        CAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGC                       >  SRR4302533.3034846/1‑94 (MQ=60)

                                                                             TCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCG                  >  SRR4302533.6160918/1‑94 (MQ=60)

                                                                              CGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGG                 <  SRR4302533.1200916/94‑1 (MQ=60)

                                                                               GTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGG                >  SRR4302533.2442670/1‑94 (MQ=60)

                                                                               GTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGG                <  SRR4302533.166465/94‑1 (MQ=60)

                                                                                TACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGT               >  SRR4302533.285069/1‑94 (MQ=60)

                                                                                TACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGC               >  SRR4302533.5601425/1‑94 (MQ=60)

                                                                                  CGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAA             <  SRR4302533.1739975/94‑1 (MQ=60)

                                                                                  CGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAA             >  SRR4302533.4569246/1‑94 (MQ=60)

                                                                                   GTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAAT            >  SRR4302533.1061714/1‑94 (MQ=60)

                                                                                    TTTGCTCGAGGTTTTGATAATGTCCTCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTACTA           <  SRR4302533.6126527/94‑1 (MQ=60)

                                                                                    TTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATA           <  SRR4302533.4834799/94‑1 (MQ=60)

                                                                                    TTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATA           <  SRR4302533.2437736/94‑1 (MQ=60)

                                                                                     TTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAG          <  SRR4302533.3467728/94‑1 (MQ=60)

                                                                                      TGTTCGAGGTTTTGATAATGTCCGCCGGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGC         <  SRR4302533.517676/94‑1 (MQ=60)

                                                                                       GCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCA        >  SRR4302533.2058924/1‑94 (MQ=60)

                                                                                        CTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAG       <  SRR4302533.2683004/94‑1 (MQ=60)

                                                                                         TCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGT      >  SRR4302533.2986608/1‑94 (MQ=60)

                                                                                         TCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGT      >  SRR4302533.2043160/1‑94 (MQ=60)

                                                                                           GAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGA    >  SRR4302533.2712860/1‑94 (MQ=60)

                                                                                            AGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGAC   <  SRR4302533.1809487/94‑1 (MQ=60)

                                                                                            AGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGAC   <  SRR4302533.5263735/94‑1 (MQ=60)

                                                                                             GGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACA  <  SRR4302533.2516768/94‑1 (MQ=60)

                                                                                             GGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACA  >  SRR4302533.96114/1‑94 (MQ=60)

                                                                                             GGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGGGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACA  >  SRR4302533.2023790/1‑94 (MQ=60)

GGAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAAGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACA  >  NC_000913/2043179‑2043365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`