Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,069,029	T→C	S169S (AGT→AGC)	yeeP →	putative histone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,069,029	0	T	C	99.5%	676.0 / ‑5.2	213	S169S (AGT→AGC)	yeeP	putative histone
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (103/109); total (103/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.34e-01

Only 100 of 234 total aligned reads displayed.

TCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGTCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGAT  >  NC_000913/2068939‑2069118

tCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGccc                                                                                         >  2:3705285/1‑93 (MQ=255)

  cGGTGGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCcccc                                                                                       <  2:7488572/93‑1 (MQ=255)

   ggTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCt                                                                                      <  2:5902594/93‑1 (MQ=255)

   ggTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCt                                                                                      >  2:1603333/1‑93 (MQ=255)

    gTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCtg                                                                                     >  2:131521/1‑93 (MQ=255)

     ttGTGTGCGTAGCGGCCCCCACCGGCTGGGAACTGGATACACTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGTCCCCtga                                                                                    <  2:6694539/93‑1 (MQ=255)

     ttGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCtga                                                                                    <  2:953555/93‑1 (MQ=255)

     ttGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCtga                                                                                    <  2:8873784/93‑1 (MQ=255)

     ttGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCtga                                                                                    >  1:894401/1‑93 (MQ=255)

      tgtgGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCtgat                                                                                   <  2:9828777/93‑1 (MQ=255)

       gtgGCCGTATCGGCCCGCACCGGCTGGGAACTGGCTACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCtgatg                                                                                  <  2:8404939/93‑1 (MQ=255)

           ccGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGGTTCCCGACCATGCCGCCAGCCCCCTGCTGAccc                                                                              >  2:7251629/1‑93 (MQ=255)

             gTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGa                                                                            <  1:2590100/93‑1 (MQ=255)

              tATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGAc                                                                           >  1:8424132/1‑93 (MQ=255)

              tATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGAc                                                                           <  2:5589230/93‑1 (MQ=255)

                tCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTg                                                                         >  1:4356509/1‑93 (MQ=255)

                tCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTg                                                                         >  2:3530744/1‑93 (MQ=255)

                tCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGATTCCCGACCATGCCGACAGCCCCCTGATGACCCGACTg                                                                         >  2:7169007/1‑93 (MQ=255)

                  cgCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCa                                                                       <  2:4003647/92‑1 (MQ=255)

                   gCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAg                                                                      <  1:1894316/93‑1 (MQ=255)

                    cccGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAgg                                                                     <  1:5856457/93‑1 (MQ=255)

                     ccGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGa                                                                    <  1:730331/93‑1 (MQ=255)

                      cGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGAc                                                                   >  1:4659207/1‑93 (MQ=255)

                       gCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACg                                                                  >  2:5163677/1‑93 (MQ=255)

                        cACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGa                                                                 <  1:9000896/93‑1 (MQ=255)

                         aCCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAg                                                                <  2:4739009/93‑1 (MQ=255)

                          ccGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGc                                                               <  1:9703258/93‑1 (MQ=255)

                          ccGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGc                                                               <  1:6516446/93‑1 (MQ=255)

                          ccGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGc                                                               >  2:9479564/1‑93 (MQ=255)

                            ggCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTg                                                             >  1:4573786/1‑93 (MQ=255)

                             gCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTgc                                                            >  2:122399/1‑93 (MQ=255)

                             gCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTgc                                                            <  1:1711432/93‑1 (MQ=255)

                                gggAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTgcgcc                                                         >  1:5161038/1‑92 (MQ=255)

                                gggAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCa                                                         <  2:5388033/93‑1 (MQ=255)

                                 ggAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCAc                                                        <  2:8394391/93‑1 (MQ=255)

                                   aaCTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACgg                                                      >  1:5036539/1‑93 (MQ=255)

                                    aCTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGa                                                     <  1:1055728/93‑1 (MQ=255)

                                    aCTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGa                                                     <  1:2325236/93‑1 (MQ=255)

                                     cTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGaa                                                    >  1:9179854/1‑93 (MQ=255)

                                       ggATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATc                                                  >  1:4008376/1‑93 (MQ=255)

                                          tACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGt                                               <  1:3584919/93‑1 (MQ=255)

                                          tACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGt                                               >  2:3376495/1‑93 (MQ=255)

                                           aCGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTc                                              <  2:7986535/93‑1 (MQ=255)

                                           aCGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTc                                              <  2:1856327/93‑1 (MQ=255)

                                            cGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTcc                                             <  2:9953885/93‑1 (MQ=255)

                                             gCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCTCTAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCg                                            <  1:6766357/93‑1 (MQ=255)

                                              cTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCgg                                           <  2:4586525/93‑1 (MQ=255)

                                              cTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCgg                                           <  1:5195979/93‑1 (MQ=255)

                                               tGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGt                                          >  2:4076552/1‑93 (MQ=255)

                                               tGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGt                                          >  2:2930372/1‑93 (MQ=255)

                                               tGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGt                                          >  2:2088565/1‑93 (MQ=255)

                                                ggTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGtc                                         >  2:3914688/1‑93 (MQ=255)

                                                 gTCAGTGCTCTCATGACAGCGCTGCCGGACCATGCAGCCGGCCCCGTGATGACCCGACTGCAGGACGAGGTGCGCACGGAATCTGTCCGGtct                                        <  1:9942860/93‑1 (MQ=255)

                                                 gTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGtct                                        >  1:185630/1‑93 (MQ=255)

                                                 gTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGtct                                        >  1:6229338/1‑93 (MQ=255)

                                                  tCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGtctc                                       >  2:1469497/1‑93 (MQ=255)

                                                   cAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCa                                      <  2:4973318/93‑1 (MQ=255)

                                                      tGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGc                                   <  1:7969869/93‑1 (MQ=255)

                                                      tGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGc                                   >  2:757355/1‑93 (MQ=255)

                                                      tGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGc                                   <  2:8587644/93‑1 (MQ=255)

                                                        cgcTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGccc                                 <  1:2708885/93‑1 (MQ=255)

                                                        cgcTCATGACAGCCCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGccc                                 <  1:7448195/93‑1 (MQ=255)

                                                           tCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTg                              >  1:1907564/1‑93 (MQ=255)

                                                           tCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTg                              >  1:3715415/1‑93 (MQ=255)

                                                            cATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGa                             <  2:6315662/93‑1 (MQ=255)

                                                            cATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGAACGGAATCTGTCCGGTCTCAGGCCCGTGa                             >  1:3870791/1‑93 (MQ=255)

                                                             aTGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGaa                            <  1:8516304/93‑1 (MQ=255)

                                                              tGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAAc                           >  2:236443/1‑93 (MQ=255)

                                                               gACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACa                          <  2:5309752/93‑1 (MQ=255)

                                                                aCAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAg                         >  1:276869/1‑93 (MQ=255)

                                                                aCAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAg                         <  1:7165943/93‑1 (MQ=255)

                                                                aCAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAg                         >  2:10032717/1‑93 (MQ=255)

                                                                 cAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGGCCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGt                        <  2:6915688/93‑1 (MQ=255)

                                                                      cTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTAcc                   <  1:3382492/93‑1 (MQ=255)

                                                                      cTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTAcc                   >  1:5752686/1‑93 (MQ=255)

                                                                       ttCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTTTCAGGCCCGTGAACAGTTTACcg                  >  2:6832082/1‑93 (MQ=255)

                                                                       ttCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcg                  <  1:1155855/93‑1 (MQ=255)

                                                                       ttCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcg                  <  2:2681833/93‑1 (MQ=255)

                                                                        tCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcgg                 <  2:42509/93‑1 (MQ=255)

                                                                        tCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcgg                 <  1:7509409/93‑1 (MQ=255)

                                                                        tCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcgg                 <  2:9026953/93‑1 (MQ=255)

                                                                         cccGACCATGGCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACATTTTACcggt                <  1:4471801/93‑1 (MQ=255)

                                                                         cccGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggt                <  2:8571173/93‑1 (MQ=255)

                                                                           cGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggtgg              >  1:8648552/1‑92 (MQ=255)

                                                                           cGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggtgc              <  1:4642051/93‑1 (MQ=255)

                                                                             aCCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggtgcgg            >  1:3264721/1‑93 (MQ=255)

                                                                             aCCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggtgcgg            >  1:2110223/1‑93 (MQ=255)

                                                                              ccATGCCGCCCGCCCCCTGCTGGCCCGGCTGCGGGCCGAGCCGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACACTTTACcggtgcggt           <  1:1429624/93‑1 (MQ=255)

                                                                              ccATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggtgcggg           >  2:2203359/1‑92 (MQ=255)

                                                                               cATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggtgcggtg          <  1:9309339/93‑1 (MQ=255)

                                                                                aTGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTgg         <  2:5022393/93‑1 (MQ=255)

                                                                                  gccgccAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGAc       <  1:5589404/93‑1 (MQ=255)

                                                                                   ccgccAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGAcc      <  1:3091186/93‑1 (MQ=255)

                                                                                     gccAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCgg    >  1:10029756/1‑93 (MQ=255)

                                                                                      ccAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGGCCGGa   >  2:1966609/1‑93 (MQ=255)

                                                                                      ccAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGa   <  1:162551/93‑1 (MQ=255)

                                                                                      ccAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGa   <  2:2821487/93‑1 (MQ=255)

                                                                                      ccAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGa   >  1:8886624/1‑93 (MQ=255)

                                                                                       cAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGat  >  1:220725/1‑93 (MQ=255)

                                                                                       cAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGat  <  1:7015071/93‑1 (MQ=255)

TCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGTCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGAT  >  NC_000913/2068939‑2069118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGTCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGATATTT  >  NC_000913/2068936‑2069122

ACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGC                                                                                               <  SRR4302540.235961/94‑1 (MQ=60)

ACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGC                                                                                               >  SRR4302540.8681346/1‑94 (MQ=60)

 CATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCCTGCCGCCAGCC                                                                                              >  SRR4302540.972177/1‑94 (MQ=60)

  ATCCGGTTGTGGCCGTATCGTCCCGCACCGGCTGGGAACTGGATACGCTGGTTAGTGCGCTCATGTCAGCGCTTCCCGACCATGCCGCCAGCCC                                                                                             >  SRR4302540.9065171/1‑94 (MQ=60)

  ATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCC                                                                                             >  SRR4302540.7404959/1‑94 (MQ=60)

  ATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCC                                                                                             >  SRR4302540.4030116/1‑94 (MQ=60)

  ATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCC                                                                                             >  SRR4302540.8808399/1‑94 (MQ=60)

   TCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCC                                                                                            <  SRR4302540.1927037/94‑1 (MQ=60)

       GTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGA                                                                                        >  SRR4302540.903907/1‑94 (MQ=60)

        TTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGAT                                                                                       <  SRR4302540.8115231/94‑1 (MQ=60)

         TGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGCTG                                                                                      >  SRR4302540.7335278/1‑94 (MQ=60)

         TGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATG                                                                                      <  SRR4302540.4693712/94‑1 (MQ=60)

           TGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGAC                                                                                    >  SRR4302540.9042669/1‑94 (MQ=60)

            GGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACC                                                                                   >  SRR4302540.2164123/1‑94 (MQ=60)

             GCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCC                                                                                  >  SRR4302540.305110/1‑94 (MQ=60)

              GCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCG                                                                                 <  SRR4302540.3352014/94‑1 (MQ=60)

                GTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGAC                                                                               <  SRR4302540.2618025/94‑1 (MQ=60)

                GTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGAC                                                                               >  SRR4302540.8521910/1‑94 (MQ=60)

                 TATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACT                                                                              >  SRR4302540.5226298/1‑94 (MQ=60)

                  ATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTG                                                                             >  SRR4302540.4403892/1‑94 (MQ=60)

                  ATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTG                                                                             >  SRR4302540.9963049/1‑94 (MQ=60)

                     GGCCCGCACCGGCTGGGAACTGGATACGCGGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAG                                                                          <  SRR4302540.3784557/94‑1 (MQ=60)

                      GCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGG                                                                         <  SRR4302540.1915049/94‑1 (MQ=60)

                      GCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGG                                                                         <  SRR4302540.2423735/94‑1 (MQ=60)

                       CCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGA                                                                        <  SRR4302540.5921225/94‑1 (MQ=60)

                       CCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGA                                                                        <  SRR4302540.5369501/94‑1 (MQ=60)

                       CCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGA                                                                        >  SRR4302540.9396024/1‑94 (MQ=60)

                       CCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGA                                                                        <  SRR4302540.8559352/94‑1 (MQ=60)

                        CCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGAC                                                                       <  SRR4302540.738150/94‑1 (MQ=60)

                        CCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGAC                                                                       <  SRR4302540.4444944/94‑1 (MQ=60)

                        CCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGAC                                                                       >  SRR4302540.4709074/1‑94 (MQ=60)

                         CGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGGCCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACG                                                                      <  SRR4302540.8493532/94‑1 (MQ=60)

                           CACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAG                                                                    >  SRR4302540.8825250/1‑94 (MQ=60)

                           CACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAG                                                                    <  SRR4302540.9108885/94‑1 (MQ=60)

                             CCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCT                                                                  <  SRR4302540.6588838/94‑1 (MQ=60)

                             CCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCT                                                                  <  SRR4302540.9817322/94‑1 (MQ=60)

                              CGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTG                                                                 >  SRR4302540.4623134/1‑94 (MQ=60)

                                GCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCG                                                               <  SRR4302540.1729919/94‑1 (MQ=60)

                                  TGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCC                                                             >  SRR4302540.5217145/1‑94 (MQ=60)

                                  TGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCA                                                             >  SRR4302540.1663698/1‑94 (MQ=60)

                                   GGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCAC                                                            >  SRR4302540.5927004/1‑94 (MQ=60)

                                   GGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCAC                                                            <  SRR4302540.4676320/94‑1 (MQ=60)

                                   GGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCAC                                                            >  SRR4302540.2056612/1‑94 (MQ=60)

                                     GAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGG                                                          >  SRR4302540.5091019/1‑94 (MQ=60)

                                     GAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGG                                                          >  SRR4302540.1827682/1‑94 (MQ=60)

                                       ACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAA                                                        <  SRR4302540.2350276/94‑1 (MQ=60)

                                       ACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAA                                                        <  SRR4302540.1066471/94‑1 (MQ=60)

                                       ACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAA                                                        >  SRR4302540.9289957/1‑94 (MQ=60)

                                       ACTGGATACGCGGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCTCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAA                                                        <  SRR4302540.1066472/94‑1 (MQ=60)

                                         TGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATC                                                      <  SRR4302540.5042495/94‑1 (MQ=60)

                                         TGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATC                                                      >  SRR4302540.9434493/1‑94 (MQ=60)

                                         TGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATC                                                      >  SRR4302540.4051533/1‑94 (MQ=60)

                                         TGGATACGCTGGTCAGTGCCCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTTATGACCCGACTGCAGGACGAGCTGCGCACGGAATC                                                      <  SRR4302540.3350658/94‑1 (MQ=60)

                                          GGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCT                                                     >  SRR4302540.746185/1‑94 (MQ=60)

                                          GGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCT                                                     <  SRR4302540.3676420/94‑1 (MQ=60)

                                           GATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTG                                                    <  SRR4302540.7572575/94‑1 (MQ=60)

                                           GATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTG                                                    >  SRR4302540.5735300/1‑94 (MQ=60)

                                             TACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTC                                                  <  SRR4302540.3184096/94‑1 (MQ=60)

                                             TACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTC                                                  <  SRR4302540.3624076/94‑1 (MQ=60)

                                               CGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCG                                                >  SRR4302540.1579644/1‑94 (MQ=60)

                                                GCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCTCTAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGG                                               <  SRR4302540.6841137/94‑1 (MQ=60)

                                                 CTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGT                                              <  SRR4302540.5252561/94‑1 (MQ=60)

                                                 CTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGT                                              >  SRR4302540.7752188/1‑94 (MQ=60)

                                                   GGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCT                                            >  SRR4302540.6298614/1‑94 (MQ=60)

                                                   GGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCT                                            >  SRR4302540.4739512/1‑94 (MQ=60)

                                                   GGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCT                                            >  SRR4302540.187475/1‑94 (MQ=60)

                                                    GTCAGTGCTCTCATGACAGCGCTGCCGGACCATGCAGCCGGCCCCGTGATGACCCGACTGCAGGACGAGGTGCGCACGGAATCTGTCCGGTCTC                                           <  SRR4302540.10058978/94‑1 (MQ=60)

                                                        GTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGC                                       >  SRR4302540.452897/1‑94 (MQ=60)

                                                        GTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGC                                       <  SRR4302540.7228506/94‑1 (MQ=60)

                                                        GTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGC                                       >  SRR4302540.2345633/1‑94 (MQ=60)

                                                         TGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCC                                      <  SRR4302540.8062737/94‑1 (MQ=60)

                                                           CGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCG                                    <  SRR4302540.2738094/94‑1 (MQ=60)

                                                           CGCTCATGACAGCCCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCG                                    <  SRR4302540.7531204/94‑1 (MQ=60)

                                                            GCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGG                                   >  SRR4302540.2629918/1‑94 (MQ=60)

                                                             CTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTG                                  >  SRR4302540.3755954/1‑94 (MQ=60)

                                                             CTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTG                                  >  SRR4302540.1928409/1‑94 (MQ=60)

                                                              TCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGA                                 <  SRR4302540.1309388/94‑1 (MQ=60)

                                                              TCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGA                                 >  SRR4302540.2751844/1‑94 (MQ=60)

                                                              TCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGAACGGAATCTGTCCGGTCTCAGGCCCGTGA                                 >  SRR4302540.3912868/1‑94 (MQ=60)

                                                              TCATGACAGCGATTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGACCTGCGAACGGAATCTGTCCGGGCTCGGGGCCGTGA                                 >  SRR4302540.3912867/1‑94 (MQ=60)

                                                               CATAACAGCCCCTCCCGACCATGACGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAA                                <  SRR4302540.3491112/94‑1 (MQ=60)

                                                                ATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAAC                               >  SRR4302540.3516706/1‑94 (MQ=60)

                                                                ATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAAC                               <  SRR4302540.8615275/94‑1 (MQ=60)

                                                                ATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAAC                               >  SRR4302540.1436757/1‑94 (MQ=60)

                                                                ATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAAC                               >  SRR4302540.2887017/1‑94 (MQ=60)

                                                                  GACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAG                             <  SRR4302540.106136/94‑1 (MQ=60)

                                                                  GACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAG                             >  SRR4302540.279592/1‑94 (MQ=60)

                                                                   ACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGT                            <  SRR4302540.7024342/94‑1 (MQ=60)

                                                                   ACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGT                            <  SRR4302540.7245835/94‑1 (MQ=60)

                                                                      GCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTA                         >  SRR4302540.4578755/1‑94 (MQ=60)

                                                                       CGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTAC                        <  SRR4302540.406447/94‑1 (MQ=60)

                                                                        GCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACC                       >  SRR4302540.5816178/1‑94 (MQ=60)

                                                                        GCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACC                       >  SRR4302540.8661136/1‑94 (MQ=60)

                                                                        GCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACC                       >  SRR4302540.5878192/1‑94 (MQ=60)

                                                                         CTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCG                      <  SRR4302540.3419414/94‑1 (MQ=60)

                                                                         CTGCCCGACCAGGCCGCCAGCCCCCTGATGGCCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCG                      <  SRR4302540.9621815/94‑1 (MQ=60)

                                                                          TTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGG                     <  SRR4302540.1167885/94‑1 (MQ=60)

                                                                           TCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGT                    <  SRR4302540.7593344/94‑1 (MQ=60)

                                                                            CCCGACCATGGCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACATTTTACCGGTG                   <  SRR4302540.4520300/94‑1 (MQ=60)

                                                                            CCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTG                   <  SRR4302540.6853375/94‑1 (MQ=60)

                                                                            CCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTG                   <  SRR4302540.9185939/94‑1 (MQ=60)

                                                                             CCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGG                  >  SRR4302540.8749017/1‑94 (MQ=60)

                                                                             CCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGC                  <  SRR4302540.1811695/94‑1 (MQ=60)

                                                                              CGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCG                 <  SRR4302540.4691748/94‑1 (MQ=60)

                                                                               GACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGG                >  SRR4302540.6743040/1‑94 (MQ=60)

                                                                               GACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGG                >  SRR4302540.2132872/1‑94 (MQ=60)

                                                                               GACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGG                >  SRR4302540.3300343/1‑94 (MQ=60)

                                                                                 CCATGCCGCCCGCCCCCTGCTGGCCCGGCTGCGGGCCGAGCCGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACACTTTACCGGTGCGGTG              <  SRR4302540.1445105/94‑1 (MQ=53)

                                                                                  CATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGCACAGTTTACCGGTGCGGTGG             >  SRR4302540.1884990/1‑94 (MQ=60)

                                                                                  CATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGG             >  SRR4302540.5192171/1‑94 (MQ=60)

                                                                                  CATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGG             <  SRR4302540.641636/94‑1 (MQ=60)

                                                                                  CATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGG             <  SRR4302540.9420481/94‑1 (MQ=60)

                                                                                   ATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGA            <  SRR4302540.3939212/94‑1 (MQ=60)

                                                                                   ATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGA            <  SRR4302540.9240118/94‑1 (MQ=60)

                                                                                   ATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGA            >  SRR4302540.6038633/1‑94 (MQ=60)

                                                                                     GCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACC          <  SRR4302540.5650916/94‑1 (MQ=60)

                                                                                      CCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCG         <  SRR4302540.3125093/94‑1 (MQ=60)

                                                                                      CCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCG         >  SRR4302540.2599750/1‑94 (MQ=60)

                                                                                       CGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGCCCGG        >  SRR4302540.2111495/1‑94 (MQ=60)

                                                                                       CGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGG        >  SRR4302540.10146435/1‑94 (MQ=60)

                                                                                       CGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGG        >  SRR4302540.5364091/1‑94 (MQ=60)

                                                                                        GCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGA       >  SRR4302540.8989501/1‑94 (MQ=60)

                                                                                         CCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGAT      <  SRR4302540.164181/94‑1 (MQ=60)

                                                                                         CCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGAT      >  SRR4302540.222868/1‑94 (MQ=60)

                                                                                          CAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGATA     <  SRR4302540.7092965/94‑1 (MQ=60)

                                                                                          CAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGACCGTGAACAGTTTACCGGTGCGGTGGACCGGATA     >  SRR4302540.6064072/1‑94 (MQ=60)

                                                                                           AGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGATAT    >  SRR4302540.1496834/1‑94 (MQ=60)

                                                                                            GCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGATATT   <  SRR4302540.140344/94‑1 (MQ=60)

                                                                                            GCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGATATT   <  SRR4302540.2416716/94‑1 (MQ=60)

                                                                                            GCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGATATT   <  SRR4302540.3984755/94‑1 (MQ=60)

                                                                                             CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGATATTT  <  SRR4302540.5116340/94‑1 (MQ=60)

ACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGTCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGATATTT  >  NC_000913/2068936‑2069122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`