Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,069,029	T→C	S169S (AGT→AGC)	yeeP →	putative histone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,069,029	0	T	C	99.5%	621.4 / NA	195	S169S (AGT→AGC)	yeeP	putative histone
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (91/103); minor base A (1/0); total (92/103)
Fisher's exact test for biased strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 209 total aligned reads displayed.

GGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGTCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGG  >  NC_000913/2068942‑2069116

ggTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCAAGACAGAGCTTCCCGAACATGGCGCCAGCCCCCt                                                                                    >  2:5574560/1‑93 (MQ=255)

 gttgttGCCGTATCTGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCtg                                                                                   <  2:2728699/93‑1 (MQ=255)

  ttGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCtga                                                                                  <  1:1575869/93‑1 (MQ=255)

    gtgGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCtgatg                                                                                <  1:3178175/93‑1 (MQ=255)

    gtgGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCtgatg                                                                                >  1:631999/1‑93 (MQ=255)

     tgGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCtgatga                                                                               <  2:3494342/93‑1 (MQ=255)

     tgGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCtgatga                                                                               <  1:6001522/93‑1 (MQ=255)

      ggCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGAc                                                                              >  2:5004811/1‑93 (MQ=255)

      ggCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGAc                                                                              <  1:896114/93‑1 (MQ=255)

      ggCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGACCCCTGATGAc                                                                              >  1:1376949/1‑93 (MQ=255)

        ccGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGAccc                                                                            >  1:5694411/1‑93 (MQ=255)

           tATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGAc                                                                         >  1:3990204/1‑93 (MQ=255)

           tATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGAc                                                                         <  1:5833980/93‑1 (MQ=255)

           tATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCCAc                                                                         >  2:2463744/1‑93 (MQ=255)

            aTCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACt                                                                        >  1:3779202/1‑93 (MQ=255)

              cGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGc                                                                      >  2:5367570/1‑93 (MQ=255)

               ggCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCa                                                                     <  1:99608/93‑1 (MQ=255)

               ggCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCa                                                                     >  1:4444983/1‑93 (MQ=255)

               ggCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCa                                                                     >  2:3245319/1‑93 (MQ=255)

                gCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAg                                                                    <  1:2801602/93‑1 (MQ=255)

                 cccGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAgg                                                                   <  2:4458976/93‑1 (MQ=255)

                  ccGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGa                                                                  <  1:1607915/93‑1 (MQ=255)

                  ccGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGa                                                                  <  1:5284281/93‑1 (MQ=255)

                   cGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGAc                                                                 <  2:4696709/93‑1 (MQ=255)

                     cACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGa                                                               >  2:280404/1‑93 (MQ=255)

                      aCCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAg                                                              >  1:2063360/1‑93 (MQ=255)

                       ccGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGc                                                             >  2:3070439/1‑93 (MQ=255)

                        cGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCt                                                            >  2:3888601/1‑93 (MQ=255)

                        cGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCt                                                            <  2:4063415/93‑1 (MQ=255)

                         ggCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTg                                                           >  2:4377498/1‑93 (MQ=255)

                          gCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTgc                                                          >  1:5053717/1‑93 (MQ=255)

                          gCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTgc                                                          >  1:4916908/1‑93 (MQ=255)

                          gCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTgc                                                          <  1:417653/93‑1 (MQ=255)

                          gCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTgc                                                          <  2:6226919/93‑1 (MQ=255)

                           cTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTgcg                                                         <  2:1236248/93‑1 (MQ=255)

                            tGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTgcgc                                                        >  2:1052071/1‑93 (MQ=255)

                            tGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTgcgc                                                        >  1:3306623/1‑93 (MQ=255)

                                     gATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCt                                               <  1:1798740/93‑1 (MQ=255)

                                        aCGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTc                                            <  1:1955608/93‑1 (MQ=255)

                                          gCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCg                                          >  1:4849730/1‑93 (MQ=255)

                                           cTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCgg                                         <  1:4991891/93‑1 (MQ=255)

                                           cTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCgg                                         >  2:2273257/1‑93 (MQ=255)

                                             ggTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGtc                                       >  2:201072/1‑93 (MQ=255)

                                             ggTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGtc                                       >  1:4668745/1‑93 (MQ=255)

                                               tCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGtctc                                     >  1:4642121/1‑93 (MQ=255)

                                                cattGCGCTCATTACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCa                                    <  2:2892948/90‑1 (MQ=255)

                                                cAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCa                                    <  2:5139220/93‑1 (MQ=255)

                                                cAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCa                                    <  1:5746211/93‑1 (MQ=255)

                                                cAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCa                                    >  1:1231464/1‑93 (MQ=255)

                                                 aGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAg                                   <  1:4695239/93‑1 (MQ=255)

                                                 aGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAg                                   >  1:1669823/1‑93 (MQ=255)

                                                 aGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAg                                   >  1:2745724/1‑93 (MQ=255)

                                                 aGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAg                                   <  1:2642651/93‑1 (MQ=255)

                                                 aGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAg                                   >  2:5866430/1‑93 (MQ=255)

                                                  gTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAgg                                  >  2:3943238/1‑93 (MQ=255)

                                                   tGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGc                                 <  2:3094578/93‑1 (MQ=255)

                                                    gcgcTCATGACAGCACTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGcc                                <  2:1845851/93‑1 (MQ=255)

                                                     cgcTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGccc                               <  1:5081846/93‑1 (MQ=255)

                                                       cTCATGACAGCGCTCCCCGACCATGCCGCGAGCCCTCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGt                             <  2:3183222/93‑1 (MQ=255)

                                                        tCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCACGTg                            >  1:1243061/1‑93 (MQ=255)

                                                          aTGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGaa                          <  2:1342303/93‑1 (MQ=255)

                                                          aTGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGaa                          >  2:5283342/1‑93 (MQ=255)

                                                           tGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAAc                         <  1:145166/93‑1 (MQ=255)

                                                            gACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACa                        <  1:849363/93‑1 (MQ=255)

                                                              cAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGt                      <  1:2201601/93‑1 (MQ=255)

                                                              cAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGt                      <  2:1788059/93‑1 (MQ=255)

                                                              cAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGt                      >  1:3576364/1‑93 (MQ=255)

                                                              cAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGt                      <  1:4783100/93‑1 (MQ=255)

                                                               aGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGtt                     <  2:5768942/93‑1 (MQ=255)

                                                                 cgcTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTa                   >  1:3866462/1‑93 (MQ=255)

                                                                 cgcTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTa                   <  2:4191487/93‑1 (MQ=255)

                                                                 cctgtCCCGACCATGCCGCCAGCCGCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCGGCCCGGTCGCAGGCCCGTGAACAGTGTa                   <  2:3544487/89‑1 (MQ=255)

                                                                  gcTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTAc                  <  2:4028495/93‑1 (MQ=255)

                                                                  gcTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTAc                  >  1:2467046/1‑93 (MQ=255)

                                                                  gcTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTAc                  <  1:3673378/93‑1 (MQ=255)

                                                                    ttCCCGCCCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcg                <  1:1001123/93‑1 (MQ=255)

                                                                    ttCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcg                <  1:6097789/93‑1 (MQ=255)

                                                                     tCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcgg               <  2:931602/93‑1 (MQ=255)

                                                                     tCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcgg               >  2:130084/1‑93 (MQ=255)

                                                                     tCCCGACCAGTCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcgg               <  2:5427713/93‑1 (MQ=255)

                                                                      cccGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggt              >  1:3394908/1‑93 (MQ=255)

                                                                      cccGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggt              <  2:611878/93‑1 (MQ=255)

                                                                       ccGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggtg             >  1:1347801/1‑93 (MQ=255)

                                                                       ccGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggtg             <  1:5430025/93‑1 (MQ=255)

                                                                        cGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggtgc            >  2:2227428/1‑93 (MQ=255)

                                                                        cGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggtgc            >  1:284928/1‑93 (MQ=255)

                                                                         gACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggtgcg           >  2:3547720/1‑93 (MQ=255)

                                                                         gACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggtgcg           <  1:5571489/93‑1 (MQ=255)

                                                                          gccgtGCCGCCAGCCCCCTAATGCCCCGACTGCAGAACGAGCTGCGCACGGAATCTGTCCGGTCTCAGCCCCGTGAACAGTTTACcggtgcgg          <  2:3642837/89‑1 (MQ=255)

                                                                           ccATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggtgcggt         <  2:850720/93‑1 (MQ=255)

                                                                           ccATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggtgcggt         <  2:1410543/93‑1 (MQ=255)

                                                                           ccATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggtgcggt         <  1:1914082/93‑1 (MQ=255)

                                                                            cATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggtgcggtg        <  1:295355/93‑1 (MQ=255)

                                                                            cATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggtgcggtg        >  2:547180/1‑93 (MQ=255)

                                                                              tGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGa      >  1:2541811/1‑93 (MQ=255)

                                                                              tGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGa      >  1:2403659/1‑93 (MQ=255)

                                                                                ccgccAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGAcc    <  1:3205088/93‑1 (MQ=255)

                                                                                ccgccAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGAcc    <  2:1376111/93‑1 (MQ=255)

                                                                                 cgccAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCg   >  1:1149365/1‑93 (MQ=255)

                                                                                  gccAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCgg  <  2:1612217/93‑1 (MQ=255)

GGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGTCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGG  >  NC_000913/2068942‑2069116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑TCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGATATT  >  NC_000913/2068937‑2069121

CATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CC                                                                                             >  SRR4302533.4698385/1‑94 (MQ=60)

     GGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTG                                                                                        <  SRR4302533.2786078/94‑1 (MQ=60)

     GGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTG                                                                                        >  SRR4302533.5146787/1‑94 (MQ=60)

     GGTTGTGGCCGTATCGGCCCGCACCGGATGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGGCAG‑CCCCCTG                                                                                        >  SRR4302533.2147982/1‑94 (MQ=60)

       TTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGAT                                                                                      <  SRR4302533.5948031/94‑1 (MQ=60)

       TTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGAT                                                                                      <  SRR4302533.4172384/94‑1 (MQ=60)

       TTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGAT                                                                                      <  SRR4302533.5495947/94‑1 (MQ=60)

       TTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGAT                                                                                      <  SRR4302533.1585356/94‑1 (MQ=60)

        TGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATG                                                                                     >  SRR4302533.635719/1‑94 (MQ=60)

         GTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGA                                                                                    <  SRR4302533.3197723/94‑1 (MQ=60)

          TGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGAC                                                                                   <  SRR4302533.6037878/94‑1 (MQ=60)

          TGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGAC                                                                                   <  SRR4302533.4007317/94‑1 (MQ=60)

          TGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑ACCCCTGATGAC                                                                                   >  SRR4302533.1385233/1‑94 (MQ=60)

           GGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACC                                                                                  <  SRR4302533.4804212/94‑1 (MQ=60)

           GGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACC                                                                                  <  SRR4302533.3068717/94‑1 (MQ=60)

           GGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACC                                                                                  <  SRR4302533.901377/94‑1 (MQ=60)

            GCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCC                                                                                 >  SRR4302533.5728800/1‑94 (MQ=60)

             CCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCG                                                                                <  SRR4302533.4941107/94‑1 (MQ=60)

               GTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGAC                                                                              >  SRR4302533.4014507/1‑94 (MQ=60)

               GTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGAC                                                                              <  SRR4302533.1080927/94‑1 (MQ=60)

                TATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACT                                                                             <  SRR4302533.5869237/94‑1 (MQ=60)

                TATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACT                                                                             >  SRR4302533.3802355/1‑94 (MQ=60)

                   CGGCCCGCACCGGCTGGGAAGTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCG                                                                          >  SRR4302533.913552/1‑94 (MQ=60)

                   CGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCA                                                                          >  SRR4302533.4471867/1‑94 (MQ=60)

                   CGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCA                                                                          >  SRR4302533.1198917/1‑94 (MQ=60)

                   CGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCA                                                                          >  SRR4302533.2455707/1‑94 (MQ=60)

                    GGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAG                                                                         <  SRR4302533.4712711/94‑1 (MQ=60)

                    GGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAG                                                                         >  SRR4302533.1002246/1‑94 (MQ=60)

                    GGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAG                                                                         >  SRR4302533.2842811/1‑94 (MQ=60)

                    GGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAG                                                                         <  SRR4302533.100224/94‑1 (MQ=60)

                     GCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGG                                                                        <  SRR4302533.2818839/94‑1 (MQ=60)

                       CCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGAC                                                                      <  SRR4302533.4562588/94‑1 (MQ=60)

                       CCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGAC                                                                      <  SRR4302533.5315937/94‑1 (MQ=60)

                       CCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGAC                                                                      <  SRR4302533.1617671/94‑1 (MQ=60)

                         GCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGC                                                                    >  SRR4302533.2033370/1‑94 (MQ=60)

                         GCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGA                                                                    >  SRR4302533.1626694/1‑94 (MQ=60)

                          CACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAG                                                                   >  SRR4302533.2076080/1‑94 (MQ=60)

                            CCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAC‑CCCCCTGATGACCCGACTGCAGGACGAGCT                                                                 <  SRR4302533.1964499/94‑1 (MQ=60)

                             CGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTG                                                                >  SRR4302533.2687501/1‑94 (MQ=60)

                             CGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTG                                                                <  SRR4302533.291331/94‑1 (MQ=60)

                              GGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGC                                                               >  SRR4302533.4946689/1‑94 (MQ=60)

                              GGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGC                                                               >  SRR4302533.5084271/1‑94 (MQ=60)

                              GGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CACCCTGATGACCCGACTGCAGGACGAGCTGC                                                               >  SRR4302533.1576315/1‑94 (MQ=60)

                               GCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCG                                                              <  SRR4302533.434352/94‑1 (MQ=60)

                               GCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCG                                                              <  SRR4302533.420158/94‑1 (MQ=60)

                               GCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCG                                                              >  SRR4302533.4990595/1‑94 (MQ=60)

                                CTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGC                                                             >  SRR4302533.3327040/1‑94 (MQ=60)

                                   GGACCTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACG                                                          <  SRR4302533.5680784/94‑1 (MQ=60)

                                          GATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTG                                                   <  SRR4302533.1809686/94‑1 (MQ=60)

                                          GATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTG                                                   <  SRR4302533.1349191/94‑1 (MQ=60)

                                           ATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGT                                                  >  SRR4302533.5569504/1‑94 (MQ=60)

                                             ACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCC                                                <  SRR4302533.1967523/94‑1 (MQ=60)

                                             ACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCC                                                <  SRR4302533.2379495/94‑1 (MQ=60)

                                             ACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCC                                                <  SRR4302533.3714828/94‑1 (MQ=60)

                                              CGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCG                                               >  SRR4302533.871418/1‑94 (MQ=60)

                                              CGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCG                                               <  SRR4302533.5110437/94‑1 (MQ=60)

                                              CGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCG                                               >  SRR4302533.4879120/1‑94 (MQ=60)

                                                CTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGT                                             <  SRR4302533.5022154/94‑1 (MQ=60)

                                                 TGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTC                                            >  SRR4302533.4697035/1‑94 (MQ=60)

                                                   GTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTC                                          >  SRR4302533.3368574/1‑94 (MQ=60)

                                                   GTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTC                                          >  SRR4302533.4670246/1‑94 (MQ=60)

                                                    gcgGTGCGCTCATGACAGCGCTTCCCGACCATGCCGACAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCA                                         <  SRR4302533.574095/91‑1 (MQ=60)

                                                    TCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCA                                         >  SRR4302533.1238836/1‑94 (MQ=60)

                                                     CAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAG                                        >  SRR4302533.2762559/1‑94 (MQ=60)

                                                     CAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAG                                        >  SRR4302533.1679912/1‑94 (MQ=60)

                                                     CAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAG                                        <  SRR4302533.5780884/94‑1 (MQ=60)

                                                     CAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAG                                        <  SRR4302533.3289473/94‑1 (MQ=60)

                                                      AGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGG                                       <  SRR4302533.2658889/94‑1 (MQ=60)

                                                      AGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGG                                       <  SRR4302533.4723691/94‑1 (MQ=60)

                                                          CGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCG                                   >  SRR4302533.2485191/1‑94 (MQ=60)

                                                          CGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCG                                   <  SRR4302533.5112559/94‑1 (MQ=60)

                                                            CTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTG                                 >  SRR4302533.3558712/1‑94 (MQ=60)

                                                            CTCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCACGTG                                 >  SRR4302533.1250483/1‑94 (MQ=60)

                                                             TCATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGA                                <  SRR4302533.1781494/94‑1 (MQ=60)

                                                              CATGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAA                               >  SRR4302533.582848/1‑94 (MQ=60)

                                                                TGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACA                             <  SRR4302533.146096/94‑1 (MQ=60)

                                                                TGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACA                             <  SRR4302533.506792/94‑1 (MQ=60)

                                                                TGACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACA                             <  SRR4302533.4502172/94‑1 (MQ=60)

                                                                 GACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAG                            <  SRR4302533.854334/94‑1 (MQ=60)

                                                                  ACAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGT                           >  SRR4302533.3598405/1‑94 (MQ=60)

                                                                   CAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTT                          <  SRR4302533.2215168/94‑1 (MQ=60)

                                                                   CAGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTT                          <  SRR4302533.4812077/94‑1 (MQ=60)

                                                                    AGCGCTTCCCGACCATGCCGCGAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTT                         <  SRR4302533.1839625/94‑1 (MQ=60)

                                                                    AGCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTT                         <  SRR4302533.726899/94‑1 (MQ=60)

                                                                     GCGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTA                        >  SRR4302533.3890065/1‑94 (MQ=60)

                                                                      CGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTAC                       >  SRR4302533.535760/1‑94 (MQ=60)

                                                                      CGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTAC                       >  SRR4302533.2482263/1‑94 (MQ=60)

                                                                      CGCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTAC                       >  SRR4302533.3116384/1‑94 (MQ=60)

                                                                       GCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACC                      >  SRR4302533.6112060/1‑94 (MQ=60)

                                                                       GCTTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACC                      <  SRR4302533.3695953/94‑1 (MQ=60)

                                                                         TTCCCGCCCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGG                    <  SRR4302533.1006968/94‑1 (MQ=60)

                                                                         TTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGG                    <  SRR4302533.1446522/94‑1 (MQ=60)

                                                                         TTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGG                    <  SRR4302533.6134617/94‑1 (MQ=60)

                                                                         TTCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGG                    <  SRR4302533.3679869/94‑1 (MQ=60)

                                                                          TCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGT                   >  SRR4302533.3415875/1‑94 (MQ=60)

                                                                          TCCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGT                   <  SRR4302533.3208981/94‑1 (MQ=60)

                                                                           CCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTG                  >  SRR4302533.1355912/1‑94 (MQ=60)

                                                                           CCCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTG                  <  SRR4302533.1243540/94‑1 (MQ=60)

                                                                            CCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGC                 <  SRR4302533.5462728/94‑1 (MQ=60)

                                                                            CCGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGC                 >  SRR4302533.286670/1‑94 (MQ=60)

                                                                             CGACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCG                <  SRR4302533.3824322/94‑1 (MQ=60)

                                                                              GACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGG               <  SRR4302533.5605162/94‑1 (MQ=60)

                                                                              GACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGAGG               >  SRR4302533.2573052/1‑94 (MQ=60)

                                                                               ACCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGT              <  SRR4302533.5754669/94‑1 (MQ=60)

                                                                                CCATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTG             <  SRR4302533.1925765/94‑1 (MQ=60)

                                                                                 CATGCCGCCAGCCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTG             >  SRR4302533.4675600/1‑94 (MQ=60)

                                                                                 CATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGG            <  SRR4302533.297158/94‑1 (MQ=60)

                                                                                 CATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCGGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGG            <  SRR4302533.1603808/94‑1 (MQ=60)

                                                                                  ATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGA           >  SRR4302533.2557458/1‑94 (MQ=60)

                                                                                  ATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGA           <  SRR4302533.6331276/94‑1 (MQ=60)

                                                                                  ATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGA           >  SRR4302533.2418503/1‑94 (MQ=60)

                                                                                  ATGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGA           >  SRR4302533.4837738/1‑94 (MQ=60)

                                                                                   TGCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGAC          <  SRR4302533.1372315/94‑1 (MQ=60)

                                                                                    GCCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACC         <  SRR4302533.1954538/94‑1 (MQ=60)

                                                                                     CCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCG        <  SRR4302533.3224821/94‑1 (MQ=60)

                                                                                     CCGCCAG‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCG        >  SRR4302533.1156231/1‑94 (MQ=60)

                                                                                           G‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGATATT  >  SRR4302533.468783/1‑94 (MQ=60)

                                                                                           G‑CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGGGGACCGGATATT  >  SRR4302533.4059636/1‑94 (MQ=60)

CATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAG‑TCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGATATT  >  NC_000913/2068937‑2069121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`