Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,123,880 T→C Q123R (CAG→CGG)  wcaH ← GDP‑mannose mannosyl hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,123,8800TC100.0% 607.6 / NA 187Q123R (CAG→CGG) wcaHGDP‑mannose mannosyl hydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (89/98);  total (89/98)

Only 100 of 200 total aligned reads displayed.

TTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAG  >  NC_000913/2123790‑2123969
                                                                                          |                                                                                         
  ctcAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTc                                                                                       <  1:2305468/93‑1 (MQ=255)
   tcAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCa                                                                                      <  2:6157327/93‑1 (MQ=255)
     aGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATc                                                                                    <  2:2450890/93‑1 (MQ=255)
      gCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATcc                                                                                   <  1:9750592/93‑1 (MQ=255)
      gCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATcc                                                                                   <  1:7524207/93‑1 (MQ=255)
      gCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATcc                                                                                   >  1:458369/1‑93 (MQ=255)
       cGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCg                                                                                  <  1:5655015/93‑1 (MQ=255)
        gagaAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCgg                                                                                 >  1:5380118/1‑93 (MQ=255)
         agaAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGc                                                                                <  2:6310222/93‑1 (MQ=255)
          gaAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCa                                                                               <  1:5614484/93‑1 (MQ=255)
          gaAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCa                                                                               >  1:984513/1‑93 (MQ=255)
            aaaTAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAga                                                                             >  2:8532695/1‑92 (MQ=255)
             aaTAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTa                                                                            <  2:1383950/93‑1 (MQ=255)
              aTAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCATCCATCGGTAATCGTCATGCCGCTCATCCCGCAGTaa                                                                           >  2:6136561/1‑93 (MQ=255)
               aaGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAAc                                                                          <  1:8305984/92‑1 (MQ=255)
               aaGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAAc                                                                          <  1:8166719/92‑1 (MQ=255)
                aGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACa                                                                         <  2:6900057/93‑1 (MQ=255)
                aGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACa                                                                         >  1:4995354/1‑93 (MQ=255)
                aGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACa                                                                         <  2:9795043/93‑1 (MQ=255)
                  gcgcGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGc                                                                       <  1:7829122/93‑1 (MQ=255)
                    gcgGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGctc                                                                     <  2:791391/93‑1 (MQ=255)
                    gcgGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGctc                                                                     <  2:77691/93‑1 (MQ=255)
                     cgGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGctct                                                                    <  1:3391278/93‑1 (MQ=255)
                     cgGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGctct                                                                    <  2:5077996/93‑1 (MQ=255)
                      ggCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCtctt                                                                   >  1:709907/1‑93 (MQ=255)
                      ggCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCtctt                                                                   >  2:482025/1‑93 (MQ=255)
                        cTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCtcttct                                                                 <  2:8735361/93‑1 (MQ=255)
                         tGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTcttctt                                                                >  1:5144746/1‑93 (MQ=255)
                         tGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTcttctt                                                                <  1:124945/93‑1 (MQ=255)
                          gTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCttcttc                                                               >  1:3158412/1‑93 (MQ=255)
                          gTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCttcttc                                                               <  1:1872178/93‑1 (MQ=255)
                           ttAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTcc                                                              >  1:6331907/1‑93 (MQ=255)
                           ttAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTcc                                                              <  2:9275090/93‑1 (MQ=255)
                             aGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGa                                                            <  1:10039944/93‑1 (MQ=255)
                             aGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGa                                                            <  2:5826023/93‑1 (MQ=255)
                               cagtaaCATTATCACTGGCTAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATa                                                          <  1:1438232/89‑1 (MQ=255)
                               aaTGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATa                                                          <  2:160354/92‑1 (MQ=255)
                                 tGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCCGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATAct                                                        >  2:7359235/1‑93 (MQ=255)
                                       ttATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAg                                                  >  1:2905975/1‑93 (MQ=255)
                                       ttATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAg                                                  >  2:1593690/1‑93 (MQ=255)
                                        tATCACTGGCGAGCAGCTCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGc                                                 <  1:9307718/93‑1 (MQ=255)
                                        tATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGc                                                 <  2:5402016/93‑1 (MQ=255)
                                          tCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGa                                               >  1:1566505/1‑93 (MQ=255)
                                          tCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGa                                               <  1:3611206/93‑1 (MQ=255)
                                          tCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGa                                               >  1:2775695/1‑93 (MQ=255)
                                           cACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGaa                                              >  2:9367594/1‑93 (MQ=255)
                                            aCTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGaaa                                             >  2:3365578/1‑93 (MQ=255)
                                            aCTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGaaa                                             >  1:592385/1‑93 (MQ=255)
                                            aCTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGaaa                                             >  1:2341827/1‑93 (MQ=255)
                                            aCTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGaaa                                             >  2:5796245/1‑93 (MQ=255)
                                             cgggCGAGCAGCGCGTCCGCCGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGaaaa                                            <  1:740731/91‑1 (MQ=255)
                                             cTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGaaaa                                            <  2:4223944/93‑1 (MQ=255)
                                              tGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAAc                                           <  2:3439486/93‑1 (MQ=255)
                                                gagAGCAGCGCGTCCGACGTCAGCCAGCGGTAATTGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCg                                         >  2:6979787/3‑93 (MQ=255)
                                                 cGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGa                                        <  1:1169490/93‑1 (MQ=255)
                                                  gAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAg                                       >  1:6875012/1‑93 (MQ=255)
                                                  gAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAg                                       >  1:7006571/1‑93 (MQ=255)
                                                   agcagcGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGGAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGc                                      >  1:3453893/1‑93 (MQ=255)
                                                   agcagcGCGTCCGACGTCAGCCAGCGGTAATCGGCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGc                                      <  1:9679368/93‑1 (MQ=255)
                                                    gcagcGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGca                                     >  1:6841468/1‑93 (MQ=255)
                                                    gcagcGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGca                                     <  2:7624522/93‑1 (MQ=255)
                                                     cagcGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGcac                                    <  2:1278459/93‑1 (MQ=255)
                                                      agcGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGcacc                                   <  1:3774939/93‑1 (MQ=255)
                                                       gcgcGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGcacca                                  >  1:3041949/1‑93 (MQ=255)
                                                       gcgcGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGcacca                                  >  1:2219791/1‑93 (MQ=255)
                                                       gcgcGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGcacca                                  >  2:3970634/1‑93 (MQ=255)
                                                       gcgcGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGcacca                                  >  2:4959644/1‑93 (MQ=255)
                                                       gcgcGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAAACGAGcacca                                  >  2:3117436/1‑93 (MQ=255)
                                                        cgcgTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGcaccac                                 <  1:6131060/93‑1 (MQ=255)
                                                          cgTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACAt                               >  2:3592385/1‑93 (MQ=255)
                                                            tCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATag                             >  1:4272705/1‑93 (MQ=255)
                                                             ccGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGATGCGAAAACCGAGCACCACATagt                            >  2:5533153/1‑93 (MQ=255)
                                                              cGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGCAAACCGAGCACCACATagtg                           >  2:1065994/1‑93 (MQ=255)
                                                              cGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATagtg                           >  2:8042232/1‑93 (MQ=255)
                                                               gACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATagtga                          <  1:5767844/93‑1 (MQ=255)
                                                               gACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATagtga                          <  2:6753884/93‑1 (MQ=255)
                                                                   tCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAgtgg                      <  1:6730464/93‑1 (MQ=255)
                                                                   tCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAgtgg                      <  2:2020773/93‑1 (MQ=255)
                                                                   tCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAgtgg                      >  2:1843126/1‑93 (MQ=255)
                                                                   tCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAgtgg                      >  2:6825841/1‑93 (MQ=255)
                                                                     agccagcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAgtggtg                    <  1:4417993/93‑1 (MQ=255)
                                                                     agccagcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAgtggtg                    <  2:3533609/93‑1 (MQ=255)
                                                                     agccagcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTGCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAgtggtg                    <  1:8450548/93‑1 (MQ=255)
                                                                       ccagcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGaa                  >  1:4056249/1‑93 (MQ=255)
                                                                        cagcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGaaa                 >  1:9787849/1‑93 (MQ=255)
                                                                        cagcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGaaa                 >  2:2343055/1‑93 (MQ=255)
                                                                        cagcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGaaa                 >  1:8807098/1‑93 (MQ=255)
                                                                         agcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAAt                >  1:2707416/1‑93 (MQ=255)
                                                                          gcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATc               <  1:8597420/93‑1 (MQ=255)
                                                                           cGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATcc              >  2:9062494/1‑93 (MQ=255)
                                                                            ggTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCg             >  2:2086596/1‑93 (MQ=255)
                                                                             gTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGt            <  2:2661189/93‑1 (MQ=255)
                                                                             gTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGt            <  2:847635/93‑1 (MQ=255)
                                                                                 tCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCa        >  1:8777558/1‑93 (MQ=255)
                                                                                     cATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAgaga    >  2:10044503/1‑93 (MQ=255)
                                                                                     cATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAgaga    >  1:2470649/1‑93 (MQ=255)
                                                                                     cATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAgaga    >  2:9842659/1‑93 (MQ=255)
                                                                                      aTGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGaa   <  2:5990099/93‑1 (MQ=255)
                                                                                       tgccgcTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAg  >  2:2760068/1‑93 (MQ=255)
                                                                                       tgccgcTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAg  <  1:3014166/93‑1 (MQ=255)
                                                                                          |                                                                                         
TTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAG  >  NC_000913/2123790‑2123969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAGTTAT  >  NC_000913/2123787‑2123973
                                                                                             |                                                                                             
CGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCC                                                                                               >  SRR4302540.6674130/1‑94 (MQ=60)
  CTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGC                                                                                             >  SRR4302540.1559822/1‑94 (MQ=60)
   TTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCT                                                                                            >  SRR4302540.4415991/1‑94 (MQ=60)
   TTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCT                                                                                            >  SRR4302540.969339/1‑94 (MQ=60)
   TTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCT                                                                                            >  SRR4302540.9883592/1‑94 (MQ=60)
   TTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCT                                                                                            <  SRR4302540.2199645/94‑1 (MQ=60)
     CTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCA                                                                                          <  SRR4302540.2330279/94‑1 (MQ=60)
       CAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATC                                                                                        <  SRR4302540.5955529/94‑1 (MQ=60)
       CAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATC                                                                                        >  SRR4302540.8032156/1‑94 (MQ=60)
        ccagtGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCC                                                                                       <  SRR4302540.2627558/89‑1 (MQ=60)
        AGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCC                                                                                       >  SRR4302540.463258/1‑94 (MQ=60)
        AGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCC                                                                                       >  SRR4302540.9920507/1‑94 (MQ=60)
         GCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCG                                                                                      <  SRR4302540.9865010/94‑1 (MQ=60)
         GCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCG                                                                                      <  SRR4302540.7608390/94‑1 (MQ=60)
         GCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCG                                                                                      >  SRR4302540.1341294/1‑94 (MQ=60)
          CGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGG                                                                                     <  SRR4302540.5717283/94‑1 (MQ=60)
          CGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGG                                                                                     >  SRR4302540.5439286/1‑94 (MQ=60)
            AGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCA                                                                                   >  SRR4302540.994580/1‑94 (MQ=60)
            AGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCA                                                                                   <  SRR4302540.5335224/94‑1 (MQ=60)
             GAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAG                                                                                  <  SRR4302540.5676248/94‑1 (MQ=60)
              AAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGT                                                                                 >  SRR4302540.376320/1‑94 (MQ=60)
              AAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCCAGCAGCGCGTCGGATGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGcaaga                                                                                 >  SRR4302540.6934244/1‑89 (MQ=60)
              AAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCCAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGT                                                                                 >  SRR4302540.6934245/1‑94 (MQ=60)
                 ATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAAC                                                                              <  SRR4302540.9808730/94‑1 (MQ=60)
                  TAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACA                                                                             >  SRR4302540.5049428/1‑94 (MQ=60)
                  AAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACA                                                                             <  SRR4302540.8261557/94‑1 (MQ=60)
                  AAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACA                                                                             <  SRR4302540.8402427/94‑1 (MQ=60)
                   AGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAG                                                                            <  SRR4302540.4214437/94‑1 (MQ=60)
                   AGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAG                                                                            <  SRR4302540.1184503/94‑1 (MQ=60)
                     GCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCT                                                                          <  SRR4302540.7920607/94‑1 (MQ=60)
                     GCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCT                                                                          >  SRR4302540.1681091/1‑94 (MQ=60)
                      CGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTC                                                                         <  SRR4302540.2961060/94‑1 (MQ=60)
                        CGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTT                                                                       >  SRR4302540.717533/1‑94 (MQ=60)
                        CGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTT                                                                       <  SRR4302540.3428276/94‑1 (MQ=60)
                        CGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTT                                                                       <  SRR4302540.3428277/94‑1 (MQ=60)
                         GGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTC                                                                      >  SRR4302540.3543612/1‑94 (MQ=60)
                           CTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTT                                                                    >  SRR4302540.5200468/1‑94 (MQ=60)
                            TGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTC                                                                   <  SRR4302540.126270/94‑1 (MQ=60)
                            TGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTC                                                                   >  SRR4302540.3192782/1‑94 (MQ=60)
                             GTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCG                                                                  >  SRR4302540.6013497/1‑94 (MQ=60)
                             GTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCC                                                                  <  SRR4302540.1892690/94‑1 (MQ=60)
                             GTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCC                                                                  >  SRR4302540.6402415/1‑94 (MQ=60)
                              TTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCG                                                                 >  SRR4302540.271831/1‑94 (MQ=60)
                              TTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCG                                                                 >  SRR4302540.7607821/1‑94 (MQ=60)
                                AGCATGATCATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGAT                                                               <  SRR4302540.9230499/94‑1 (MQ=60)
                                AGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGAT                                                               <  SRR4302540.10156686/94‑1 (MQ=60)
                                  cagtAACATTATCACTGGCTAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATAC                                                             <  SRR4302540.1453836/90‑1 (MQ=60)
                                  CATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATAC                                                             >  SRR4302540.6888477/1‑94 (MQ=60)
                                  CATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATAC                                                             <  SRR4302540.4684135/94‑1 (MQ=60)
                                   ATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACT                                                            >  SRR4302540.2344842/1‑94 (MQ=60)
                                   ATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACT                                                            <  SRR4302540.8263825/94‑1 (MQ=60)
                                   ATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACT                                                            >  SRR4302540.7057301/1‑94 (MQ=60)
                                    TGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTC                                                           >  SRR4302540.2822874/1‑94 (MQ=60)
                                     GAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCT                                                          >  SRR4302540.5951532/1‑94 (MQ=60)
                                     GAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCAATCGGTAACCGCCATGCCGCTCATCCGGCAGTAACAACTCTTCTCCCTATACTCG                                                          >  SRR4302540.5951530/1‑94 (MQ=60)
                                        CATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAA                                                       <  SRR4302540.6758421/94‑1 (MQ=60)
                                         ATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAG                                                      >  SRR4302540.2937702/1‑94 (MQ=60)
                                           TATCACTGGCGAGCAGCTCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCG                                                    <  SRR4302540.9418855/94‑1 (MQ=60)
                                            ATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGA                                                   >  SRR4302540.2805686/1‑94 (MQ=60)
                                            ATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGA                                                   >  SRR4302540.1583690/1‑94 (MQ=60)
                                            ATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGA                                                   >  SRR4302540.2805687/1‑94 (MQ=60)
                                             TCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAA                                                  <  SRR4302540.3650681/94‑1 (MQ=60)
                                              CACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAA                                                 <  SRR4302540.3150159/94‑1 (MQ=60)
                                              CACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAA                                                 >  SRR4302540.598758/1‑94 (MQ=60)
                                              CACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAA                                                 >  SRR4302540.2367060/1‑94 (MQ=60)
                                                ttggctagcagcgcggccgacgactgccatcgggaacctgcttttTGCTCAGCCGGCAGTAACAGCTCTTCTGCCGATACTCGGAAGCGATAAC                                               >  SRR4302540.9046484/46‑94 (MQ=48)
                                                CGGGCGAGCAGCGCGTCCGCCGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAAC                                               <  SRR4302540.748641/94‑1 (MQ=60)
                                                    CGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAG                                           >  SRR4302540.7084333/1‑94 (MQ=60)
                                                    CGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAG                                           >  SRR4302540.6951207/1‑94 (MQ=60)
                                                    CGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAG                                           <  SRR4302540.1181602/94‑1 (MQ=60)
                                                     GAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGGAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGC                                          >  SRR4302540.3491475/1‑94 (MQ=60)
                                                      AGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCA                                         >  SRR4302540.6917251/1‑94 (MQ=60)
                                                      AGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGGCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCA                                         <  SRR4302540.9793254/94‑1 (MQ=60)
                                                        CAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACC                                       >  SRR4302540.7681100/1‑94 (MQ=60)
                                                         AGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCA                                      <  SRR4302540.2275216/94‑1 (MQ=60)
                                                         AGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCA                                      >  SRR4302540.3075331/1‑94 (MQ=60)
                                                         AGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCA                                      >  SRR4302540.2243726/1‑94 (MQ=60)
                                                         AGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCA                                      <  SRR4302540.3816124/94‑1 (MQ=60)
                                                          GCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCAC                                     <  SRR4302540.5682045/94‑1 (MQ=60)
                                                           CGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACA                                    <  SRR4302540.6199347/94‑1 (MQ=60)
                                                           CGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACA                                    <  SRR4302540.8573687/94‑1 (MQ=60)
                                                              GTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAG                                 >  SRR4302540.4319170/1‑94 (MQ=60)
                                                                  GACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAG                             <  SRR4302540.5831538/94‑1 (MQ=60)
                                                                   ACGTCAGCCAGGGGTAATCGTCATGCTGCTCATCCGGCAGGAACAGCTCTTCTTCCGATACTCTTAAGCGAAAACCGAGCACCACATAGTGAGT                            >  SRR4302540.2792406/1‑94 (MQ=60)
                                                                      TCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGT                         <  SRR4302540.5640738/94‑1 (MQ=60)
                                                                      TCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGT                         <  SRR4302540.6804775/94‑1 (MQ=60)
                                                                        AGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGA                       <  SRR4302540.8548655/94‑1 (MQ=60)
                                                                        AGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGA                       <  SRR4302540.4465863/94‑1 (MQ=60)
                                                                        AGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGA                       >  SRR4302540.7110813/1‑94 (MQ=60)
                                                                        AGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTGCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGA                       <  SRR4302540.8548656/94‑1 (MQ=60)
                                                                         GCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAA                      >  SRR4302540.4099981/1‑94 (MQ=60)
                                                                          CCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAA                     >  SRR4302540.9902525/1‑94 (MQ=60)
                                                                          CCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAA                     >  SRR4302540.8908917/1‑94 (MQ=60)
                                                                           CAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAAT                    >  SRR4302540.2736615/1‑94 (MQ=60)
                                                                             GCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCC                  <  SRR4302540.8697349/94‑1 (MQ=60)
                                                                              CGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCG                 >  SRR4302540.7306504/1‑94 (MQ=60)
                                                                               GGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGT                >  SRR4302540.3952341/1‑94 (MQ=60)
                                                                                GTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTG               >  SRR4302540.1079630/1‑94 (MQ=60)
                                                                                 TAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGC              <  SRR4302540.8896797/94‑1 (MQ=60)
                                                                                   ATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCA            >  SRR4302540.8879212/1‑94 (MQ=60)
                                                                                   ATCGTCATGCCGCTCATCAGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCA            >  SRR4302540.88551/1‑94 (MQ=60)
                                                                                     CGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGA          >  SRR4302540.3226073/1‑94 (MQ=60)
                                                                                      GTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAG         >  SRR4302540.2373795/1‑94 (MQ=60)
                                                                                       TCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGA        <  SRR4302540.4594099/94‑1 (MQ=60)
                                                                                       TCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGA        >  SRR4302540.2497247/1‑94 (MQ=60)
                                                                                        CATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGCA       >  SRR4302540.306306/1‑94 (MQ=60)
                                                                                        CATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAA       >  SRR4302540.5194629/1‑94 (MQ=60)
                                                                                         ATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAG      >  SRR4302540.6332536/1‑94 (MQ=60)
                                                                                          TGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAGT     >  SRR4302540.5566896/1‑94 (MQ=60)
                                                                                          TGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAGT     <  SRR4302540.3047309/94‑1 (MQ=60)
                                                                                           GCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAGTT    <  SRR4302540.921198/94‑1 (MQ=60)
                                                                                             CGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAGTTAT  >  SRR4302540.5422273/1‑94 (MQ=60)
                                                                                             |                                                                                             
CGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAGTTAT  >  NC_000913/2123787‑2123973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑