Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,170,345 C→T G184E (GGA→GAA)  gatD ← galactitol‑1‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,170,3450CT99.7% 1420.6 / ‑5.8 387G184E (GGA→GAA) gatDgalactitol‑1‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (189/196);  total (189/198)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.03e-01

Only 100 of 400 total aligned reads displayed.

TCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAA  >  NC_000913/2170255‑2170434
                                                                                          |                                                                                         
   cTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAgc                                                                                      >  1:4216455/1‑93 (MQ=255)
    tACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAgcg                                                                                     >  1:7639699/1‑93 (MQ=255)
    tACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAgcg                                                                                     >  1:294740/1‑93 (MQ=255)
    tACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAgcg                                                                                     >  1:2149842/1‑93 (MQ=255)
     aCTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAgcgc                                                                                    >  2:9263613/1‑93 (MQ=255)
     aCTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAgcgc                                                                                    >  2:2098108/1‑93 (MQ=255)
      cTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGcgcg                                                                                   >  2:2442317/1‑93 (MQ=255)
       tGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGa                                                                                  >  1:4358188/1‑93 (MQ=255)
       tGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGa                                                                                  >  1:3904986/1‑93 (MQ=255)
       tGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGa                                                                                  >  1:3784214/1‑93 (MQ=255)
       tGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGa                                                                                  >  1:9803120/1‑93 (MQ=255)
         ttAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACa                                                                                >  1:8527356/1‑93 (MQ=255)
          tAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAg                                                                               >  2:5866380/1‑93 (MQ=255)
           aaaTGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAgc                                                                              <  2:4143320/93‑1 (MQ=255)
           aaaTGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAgc                                                                              >  1:3425320/1‑93 (MQ=255)
             aTGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAgcgc                                                                            >  1:4539326/1‑93 (MQ=255)
               ttttCCATCGCGCAGAAAGTTTTTGCAATTCCGAGTTTTTCGAACCTAATGTCGCCCGCCGTCACCCTTTTTGCTCCCAGCGCGACGGCGCAc                                                                          <  2:5570256/92‑1 (MQ=255)
                tttGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACt                                                                         <  1:6693339/93‑1 (MQ=255)
                  tGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGa                                                                       <  1:622673/93‑1 (MQ=255)
                    cATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAAt                                                                     <  1:1161667/93‑1 (MQ=255)
                     aTCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATg                                                                    >  1:2624574/1‑93 (MQ=255)
                      tCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATgg                                                                   >  2:2953786/1‑93 (MQ=255)
                      tCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATgg                                                                   >  2:9806379/1‑93 (MQ=255)
                      tCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATgg                                                                   <  1:4075187/93‑1 (MQ=255)
                       cGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGc                                                                  <  1:3109989/93‑1 (MQ=255)
                        gCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGcc                                                                 <  2:5681328/93‑1 (MQ=255)
                          aCCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCcag                                                               >  1:7807185/1‑93 (MQ=255)
                            cGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCcagca                                                             <  1:4139379/93‑1 (MQ=255)
                            cGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCcagca                                                             >  1:1604195/1‑93 (MQ=255)
                             gAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCcagcag                                                            >  2:216508/1‑93 (MQ=255)
                             gAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCcagcag                                                            >  1:5575152/1‑93 (MQ=255)
                             gAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCcagcag                                                            >  1:2678259/1‑93 (MQ=255)
                              aaaGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAgg                                                           >  2:3199419/1‑93 (MQ=255)
                               aaGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGc                                                          <  2:7452720/93‑1 (MQ=255)
                                aGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGcc                                                         <  2:5441448/93‑1 (MQ=255)
                                 gATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCa                                                        >  1:2724227/1‑93 (MQ=255)
                                 gATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCa                                                        <  1:5393491/93‑1 (MQ=255)
                                 gATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCC‑TCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGccga                                                       <  2:7687497/93‑3 (MQ=255)
                                  aTTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCaa                                                       <  1:1686786/93‑1 (MQ=255)
                                   ttttGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAAt                                                      >  2:4687145/1‑93 (MQ=255)
                                   ttatgccagtgccagTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAAt                                                      >  2:6559518/4‑93 (MQ=255)
                                     ttGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATgg                                                    <  2:141777/93‑1 (MQ=255)
                                     ttGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATgg                                                    <  1:8397905/93‑1 (MQ=255)
                                     ttGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACCGAATGGCCAGCAGGCCAATgg                                                    >  2:3437647/1‑93 (MQ=255)
                                     ttGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATgg                                                    <  2:6950823/93‑1 (MQ=255)
                                        ccagtgccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTc                                                 <  2:8621979/93‑1 (MQ=255)
                                        ccagtgccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTc                                                 <  1:488515/93‑1 (MQ=255)
                                         cagtgccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTcc                                                <  1:9113317/93‑1 (MQ=255)
                                         cagtgccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTcc                                                >  1:5668533/1‑93 (MQ=255)
                                          agtgccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCg                                               <  2:3870257/93‑1 (MQ=255)
                                           gtgccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCgg                                              <  2:907926/93‑1 (MQ=255)
                                            tgccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGc                                             <  1:878885/93‑1 (MQ=255)
                                               cagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCAcc                                          >  2:6186263/1‑93 (MQ=255)
                                                agtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGCCAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCAcaa                                         >  1:8253312/1‑91 (MQ=255)
                                                 gtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCaa                                        <  1:3267747/93‑1 (MQ=255)
                                                  tttttCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCaat                                       <  1:1441961/93‑1 (MQ=255)
                                                  tttttCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCaat                                       <  2:5282286/93‑1 (MQ=255)
                                                  tttttCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGAACTGAATAGGCAGCAGGCCAATGGTTCCGCCACCaaa                                       >  1:2011335/1‑92 (MQ=255)
                                                   ttttCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCaata                                      <  2:1945858/93‑1 (MQ=255)
                                                   ttttCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCaata                                      <  2:4015869/93‑1 (MQ=255)
                                                    tttCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCaataa                                     <  2:2713867/93‑1 (MQ=255)
                                                      tCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCaataata                                   >  1:7985230/1‑93 (MQ=255)
                                                         gAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAataataata                                >  2:7161840/1‑93 (MQ=255)
                                                          aaCTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAAtaataataa                               <  1:6375236/93‑1 (MQ=255)
                                                           aCTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAAc                              >  1:5997760/1‑93 (MQ=255)
                                                           aCTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAAc                              <  2:8326319/93‑1 (MQ=255)
                                                             tAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGt                            <  1:82276/93‑1 (MQ=255)
                                                              aaTGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGtt                           >  1:2370504/1‑93 (MQ=255)
                                                               aTGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGttt                          >  1:9221936/1‑93 (MQ=255)
                                                               aTGGCGATCGCCGTCACAGTCTTTGCTGCCAGCGCGTCAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGttt                          >  2:7069017/1‑93 (MQ=255)
                                                                tGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGtttt                         <  1:10022288/93‑1 (MQ=255)
                                                                tGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGtttt                         <  1:7398735/93‑1 (MQ=255)
                                                                  tCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTa                       <  1:682013/93‑1 (MQ=255)
                                                                   cGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTAt                      <  1:1058397/93‑1 (MQ=255)
                                                                    gATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTAtt                     <  1:9306654/93‑1 (MQ=255)
                                                                    gATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTAtt                     <  1:7283973/93‑1 (MQ=255)
                                                                     aTCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATCACGTTTTTAttt                    >  2:7630197/1‑92 (MQ=255)
                                                                     aTCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATtc                    >  2:2556968/1‑93 (MQ=255)
                                                                     aTCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATtc                    >  2:9449409/1‑93 (MQ=255)
                                                                     aTCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATtc                    <  2:8283055/93‑1 (MQ=255)
                                                                      tCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATtct                   <  1:988159/93‑1 (MQ=255)
                                                                      tCGCCGTCACACTCTATGCTTCCAGCGCGACAGCGCACTGAATGACCAGCGGGCAAATGTTCCCGGCACCAATAATAATATCGTTTTTATtct                   <  2:7894533/93‑1 (MQ=255)
                                                                        gCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTca                 >  1:952375/1‑93 (MQ=255)
                                                                         ccGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTcac                <  2:2743829/93‑1 (MQ=255)
                                                                         ccGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTcac                >  2:3661568/1‑93 (MQ=255)
                                                                         ccGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTcac                <  1:6998446/93‑1 (MQ=255)
                                                                         ccGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTcac                >  1:4718952/1‑93 (MQ=255)
                                                                          cGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTcaca               >  2:4981131/1‑93 (MQ=255)
                                                                          cGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTcaca               >  2:936384/1‑93 (MQ=255)
                                                                            tCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAAc             <  2:8758259/93‑1 (MQ=255)
                                                                            tCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAAc             >  2:1082392/1‑93 (MQ=255)
                                                                              acacTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCt           >  1:1324882/1‑93 (MQ=255)
                                                                              acacTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCt           <  2:9691943/93‑1 (MQ=255)
                                                                               cacTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCtt          <  2:6313605/93‑1 (MQ=255)
                                                                               cacTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCtt          <  2:332937/93‑1 (MQ=255)
                                                                                 ctctTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTgc        >  2:6700564/1‑93 (MQ=255)
                                                                                  tctTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATACTAACGTTTTTATTCTCACAACCTTgcg       >  1:5170883/1‑93 (MQ=255)
                                                                                    tttGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCt     >  2:1825999/1‑93 (MQ=255)
                                                                                      tGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTaa   >  2:4819219/1‑93 (MQ=255)
                                                                                      tGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTaa   <  2:4446949/93‑1 (MQ=255)
                                                                                          |                                                                                         
TCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAA  >  NC_000913/2170255‑2170434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 218 total aligned reads displayed.
ATTTCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAA  >  NC_000913/2170252‑2170438
                                                                                             |                                                                                             
ATTTCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTT                                                                                               >  SRR4302540.354908/1‑94 (MQ=60)
ATTTCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTT                                                                                               >  SRR4302540.6832874/1‑94 (MQ=60)
 TTTCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTC                                                                                              <  SRR4302540.5489801/94‑1 (MQ=60)
   TCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCA                                                                                            >  SRR4302540.2470694/1‑94 (MQ=60)
    CGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAG                                                                                           <  SRR4302540.2711411/94‑1 (MQ=60)
    CGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAG                                                                                           <  SRR4302540.836893/94‑1 (MQ=60)
    CGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAG                                                                                           >  SRR4302540.4327287/1‑94 (MQ=60)
    CGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAG                                                                                           <  SRR4302540.145119/94‑1 (MQ=60)
     GCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGC                                                                                          >  SRR4302540.8859453/1‑94 (MQ=60)
     GCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGC                                                                                          <  SRR4302540.6828690/94‑1 (MQ=60)
     GCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGC                                                                                          >  SRR4302540.8859454/1‑94 (MQ=60)
     GCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGC                                                                                          >  SRR4302540.4262360/1‑94 (MQ=60)
     GCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGC                                                                                          <  SRR4302540.152980/94‑1 (MQ=60)
      CTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCG                                                                                         >  SRR4302540.1152652/1‑94 (MQ=60)
      CTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCG                                                                                         >  SRR4302540.297650/1‑94 (MQ=60)
      CTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCG                                                                                         >  SRR4302540.7725669/1‑94 (MQ=60)
       TACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGC                                                                                        >  SRR4302540.8339204/1‑94 (MQ=60)
       TACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGC                                                                                        >  SRR4302540.5867378/1‑94 (MQ=60)
         CTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGA                                                                                      >  SRR4302540.4405587/1‑94 (MQ=60)
         CTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGA                                                                                      >  SRR4302540.4908942/1‑94 (MQ=60)
         CTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGA                                                                                      >  SRR4302540.3947329/1‑94 (MQ=60)
         CTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGA                                                                                      >  SRR4302540.3825486/1‑94 (MQ=60)
         CTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGA                                                                                      >  SRR4302540.2623380/1‑94 (MQ=60)
         CTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGA                                                                                      >  SRR4302540.9917879/1‑94 (MQ=60)
         CTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGA                                                                                      >  SRR4302540.943702/1‑94 (MQ=60)
          TGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGAC                                                                                     >  SRR4302540.10105520/1‑94 (MQ=60)
          TGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGAC                                                                                     >  SRR4302540.8246203/1‑94 (MQ=60)
           GTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACA                                                                                    >  SRR4302540.8626439/1‑94 (MQ=60)
            TTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAG                                                                                   >  SRR4302540.6230014/1‑94 (MQ=60)
             TAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGC                                                                                  >  SRR4302540.4864189/1‑94 (MQ=60)
             TAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGC                                                                                  >  SRR4302540.3462557/1‑94 (MQ=60)
              AAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCG                                                                                 <  SRR4302540.318243/94‑1 (MQ=60)
              AAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCG                                                                                 >  SRR4302540.8660801/1‑94 (MQ=60)
               AATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGC                                                                                >  SRR4302540.4588455/1‑94 (MQ=60)
               AATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGC                                                                                >  SRR4302540.4588454/1‑94 (MQ=60)
               AATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGC                                                                                >  SRR4302540.4763465/1‑94 (MQ=60)
               AATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGC                                                                                >  SRR4302540.9981111/1‑94 (MQ=60)
                ATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCA                                                                               >  SRR4302540.9353368/1‑94 (MQ=60)
                  TTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACT                                                                             <  SRR4302540.3159991/94‑1 (MQ=60)
                  GTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACT                                                                             >  SRR4302540.2465665/1‑94 (MQ=60)
                   TTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTG                                                                            <  SRR4302540.6767232/94‑1 (MQ=60)
                    TTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGA                                                                           <  SRR4302540.1696099/94‑1 (MQ=60)
                     TGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAA                                                                          <  SRR4302540.6737657/94‑1 (MQ=60)
                     TGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAA                                                                          <  SRR4302540.9000446/94‑1 (MQ=60)
                      GCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAAT                                                                         >  SRR4302540.1605662/1‑94 (MQ=60)
                      GCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAAT                                                                         >  SRR4302540.4614011/1‑94 (MQ=60)
                      GCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAAT                                                                         <  SRR4302540.120214/94‑1 (MQ=60)
                      GCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAAT                                                                         >  SRR4302540.9568015/1‑94 (MQ=60)
                       CATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATG                                                                        <  SRR4302540.1173731/94‑1 (MQ=60)
                       CATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATG                                                                        <  SRR4302540.6371703/94‑1 (MQ=60)
                       CATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATG                                                                        >  SRR4302540.4497736/1‑94 (MQ=60)
                       CATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATG                                                                        >  SRR4302540.2652959/1‑94 (MQ=60)
                       CATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATG                                                                        >  SRR4302540.2658200/1‑94 (MQ=60)
                        ATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGG                                                                       >  SRR4302540.1667078/1‑94 (MQ=60)
                        ATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGG                                                                       >  SRR4302540.2696339/1‑94 (MQ=60)
                        ATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGG                                                                       <  SRR4302540.9924431/94‑1 (MQ=60)
                         TCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGC                                                                      <  SRR4302540.4119113/94‑1 (MQ=60)
                          CGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCC                                                                     >  SRR4302540.2112453/1‑94 (MQ=60)
                            CACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAG                                                                   >  SRR4302540.7898419/1‑94 (MQ=60)
                            CACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAG                                                                   <  SRR4302540.8615856/94‑1 (MQ=60)
                             ACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGC                                                                  >  SRR4302540.4239517/1‑94 (MQ=60)
                             ACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGC                                                                  >  SRR4302540.150094/1‑94 (MQ=60)
                             ACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGC                                                                  >  SRR4302540.5426649/1‑94 (MQ=60)
                             ACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGC                                                                  >  SRR4302540.4239516/1‑94 (MQ=60)
                              CCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCA                                                                 >  SRR4302540.1621745/1‑94 (MQ=60)
                              CCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCA                                                                 >  SRR4302540.3602594/1‑94 (MQ=60)
                              CCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCA                                                                 <  SRR4302540.6513767/94‑1 (MQ=60)
                              CCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCA                                                                 <  SRR4302540.6513766/94‑1 (MQ=60)
                               CGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAG                                                                >  SRR4302540.5636529/1‑94 (MQ=60)
                               CGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAG                                                                <  SRR4302540.2911004/94‑1 (MQ=60)
                               CGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAG                                                                <  SRR4302540.4184172/94‑1 (MQ=60)
                               CGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAG                                                                <  SRR4302540.2079623/94‑1 (MQ=60)
                               CGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGAGCGACAGCGCTCTGAATGacgcgcag                                                                >  SRR4302540.5636528/1‑86 (MQ=60)
                                GAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCTACAGCGCACTGAATGGCCAGCAGG                                                               <  SRR4302540.9779487/94‑1 (MQ=60)
                                GAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGG                                                               <  SRR4302540.10146091/94‑1 (MQ=60)
                                GAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGG                                                               >  SRR4302540.9938583/1‑94 (MQ=60)
                                GAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGG                                                               >  SRR4302540.9547953/1‑94 (MQ=60)
                                GAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGG                                                               <  SRR4302540.5520723/94‑1 (MQ=60)
                                  AAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCC                                                             <  SRR4302540.770759/94‑1 (MQ=60)
                                   AGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCA                                                            >  SRR4302540.2753601/1‑94 (MQ=60)
                                    GATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAA                                                           <  SRR4302540.5452793/94‑1 (MQ=60)
                                    GATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAA                                                           >  SRR4302540.8519630/1‑94 (MQ=60)
                                    GATTTTGACAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAA                                                           <  SRR4302540.5452792/94‑1 (MQ=60)
                                     ATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAAT                                                          <  SRR4302540.6255384/94‑1 (MQ=60)
                                     ATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAAT                                                          <  SRR4302540.1705082/94‑1 (MQ=60)
                                      TTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATG                                                         <  SRR4302540.23899/94‑1 (MQ=60)
                                      TTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATG                                                         <  SRR4302540.3362896/94‑1 (MQ=60)
                                        TTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGT                                                       <  SRR4302540.8495364/94‑1 (MQ=60)
                                         TGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTT                                                      >  SRR4302540.2721544/1‑94 (MQ=60)
                                         TGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTT                                                      >  SRR4302540.3090762/1‑94 (MQ=60)
                                           CCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCC                                                    <  SRR4302540.493745/94‑1 (MQ=60)
                                           CCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCC                                                    <  SRR4302540.6971762/94‑1 (MQ=60)
                                           CCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCC                                                    >  SRR4302540.5730956/1‑94 (MQ=60)
                                           CCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCC                                                    <  SRR4302540.7172094/94‑1 (MQ=60)
                                           CCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCC                                                    <  SRR4302540.8327236/94‑1 (MQ=60)
                                           CCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATCGCCAGCAGGCCAATGGTTCC                                                    <  SRR4302540.9855707/94‑1 (MQ=60)
                                            CAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCG                                                   <  SRR4302540.7404418/94‑1 (MQ=60)
                                            CAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCG                                                   >  SRR4302540.7502577/1‑94 (MQ=60)
                                            CAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCG                                                   <  SRR4302540.9222841/94‑1 (MQ=60)
                                             AGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGG                                                  <  SRR4302540.2653934/94‑1 (MQ=60)
                                             AGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGG                                                  >  SRR4302540.8377851/1‑94 (MQ=60)
                                             AGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGG                                                  >  SRR4302540.1159350/1‑94 (MQ=60)
                                              GTGCCAGTTTTTCTGAACTAATTGCGATCGCCGTCAGACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTTCGGC                                                 >  SRR4302540.1859192/1‑94 (MQ=60)
                                              GTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGC                                                 <  SRR4302540.5325467/94‑1 (MQ=60)
                                              GTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGC                                                 >  SRR4302540.2068196/1‑94 (MQ=60)
                                              GTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGC                                                 <  SRR4302540.6177652/94‑1 (MQ=60)
                                               TGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCA                                                <  SRR4302540.5533291/94‑1 (MQ=60)
                                               TGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCA                                                <  SRR4302540.8444578/94‑1 (MQ=60)
                                                GCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCAC                                               >  SRR4302540.9310104/1‑94 (MQ=60)
                                                GCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCAC                                               >  SRR4302540.9310108/1‑94 (MQ=60)
                                                  CAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCA                                             <  SRR4302540.3970547/94‑1 (MQ=60)
                                                  CAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCA                                             <  SRR4302540.1109348/94‑1 (MQ=60)
                                                  CAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCACCAGGCCAATGGTTCCGGCAACA                                             >  SRR4302540.8349238/1‑94 (MQ=60)
                                                   AGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAA                                            <  SRR4302540.6027854/94‑1 (MQ=60)
                                                   AGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAA                                            <  SRR4302540.3158907/94‑1 (MQ=60)
                                                    GTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAAT                                           >  SRR4302540.9774911/1‑94 (MQ=60)
                                                    GTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAAT                                           <  SRR4302540.3303410/94‑1 (MQ=60)
                                                    GTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGAACTGAATAGGCAGCAGGCCAATGGTTCCGCCACCAAA                                           >  SRR4302540.2033087/1‑94 (MQ=60)
                                                     TTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATA                                          <  SRR4302540.1457615/94‑1 (MQ=60)
                                                     TTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATA                                          >  SRR4302540.427456/1‑94 (MQ=60)
                                                     TTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATA                                          <  SRR4302540.1457614/94‑1 (MQ=60)
                                                      TTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAA                                         <  SRR4302540.9253853/94‑1 (MQ=60)
                                                       TTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAAT                                        >  SRR4302540.10006959/1‑94 (MQ=60)
                                                       TTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAAT                                        >  SRR4302540.1411065/1‑94 (MQ=60)
                                                       TTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAAT                                        <  SRR4302540.5047058/94‑1 (MQ=60)
                                                        TTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATA                                       >  SRR4302540.8078220/1‑94 (MQ=60)
                                                          CTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAAT                                     >  SRR4302540.5669166/1‑94 (MQ=60)
                                                            GAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAA                                   >  SRR4302540.3879620/1‑94 (MQ=60)
                                                            GAACTAATGGCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAA                                   <  SRR4302540.4170115/94‑1 (MQ=60)
                                                             AACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAAC                                  <  SRR4302540.6446218/94‑1 (MQ=60)
                                                             AACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAAC                                  <  SRR4302540.2330351/94‑1 (MQ=60)
                                                             AACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAAC                                  <  SRR4302540.7662663/94‑1 (MQ=60)
                                                             AACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAAC                                  >  SRR4302540.6064226/1‑94 (MQ=60)
                                                             AACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAAC                                  <  SRR4302540.8133268/94‑1 (MQ=60)
                                                              ACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACG                                 >  SRR4302540.6438000/1‑94 (MQ=60)
                                                              ACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACG                                 >  SRR4302540.4227987/1‑94 (MQ=60)
                                                               CTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGT                                <  SRR4302540.4124696/94‑1 (MQ=60)
                                                                TAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTT                               <  SRR4302540.83140/94‑1 (MQ=60)
                                                                TAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTT                               >  SRR4302540.1276830/1‑94 (MQ=60)
                                                                TAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTT                               >  SRR4302540.2396036/1‑94 (MQ=60)
                                                                 AATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTT                              <  SRR4302540.3990718/94‑1 (MQ=60)
                                                                 AATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTT                              >  SRR4302540.9332526/1‑94 (MQ=60)
                                                                 AATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTT                              <  SRR4302540.307820/94‑1 (MQ=60)
                                                                  ATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTT                             >  SRR4302540.1059070/1‑94 (MQ=60)
                                                                  ATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTT                             <  SRR4302540.3851879/94‑1 (MQ=60)
                                                                  ATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTT                             <  SRR4302540.344990/94‑1 (MQ=60)
                                                                  ATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTT                             <  SRR4302540.9332963/94‑1 (MQ=60)
                                                                  ATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTT                             >  SRR4302540.5212423/1‑94 (MQ=60)
                                                                   TGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTT                            <  SRR4302540.7481167/94‑1 (MQ=60)
                                                                   TGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTT                            <  SRR4302540.10138918/94‑1 (MQ=60)
                                                                   TGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTT                            <  SRR4302540.2389589/94‑1 (MQ=60)
                                                                    GTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTA                           >  SRR4302540.7034092/1‑94 (MQ=60)
                                                                    GTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTA                           >  SRR4302540.4532757/1‑94 (MQ=60)
                                                                     TCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTAT                          <  SRR4302540.3988273/94‑1 (MQ=60)
                                                                     TCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTAT                          <  SRR4302540.689321/94‑1 (MQ=60)
                                                                      CGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATT                         <  SRR4302540.1069173/94‑1 (MQ=60)
                                                                      CGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCAGGAACCAATAATAATAACGTTTCTATT                         >  SRR4302540.6428796/1‑94 (MQ=60)
                                                                       GCTCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTC                        <  SRR4302540.9417787/94‑1 (MQ=60)
                                                                       GATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTC                        <  SRR4302540.7365256/94‑1 (MQ=60)
                                                                       GATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTC                        <  SRR4302540.7365257/94‑1 (MQ=60)
                                                                       GATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTC                        >  SRR4302540.342607/1‑94 (MQ=60)
                                                                       GATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAACGGTTCCGGCACCAATAATAATAACGTTTTTATTT                        >  SRR4302540.2824104/1‑94 (MQ=60)
                                                                        ATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCT                       >  SRR4302540.1913731/1‑94 (MQ=60)
                                                                        ATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCT                       <  SRR4302540.8071092/94‑1 (MQ=60)
                                                                         TCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTC                      >  SRR4302540.434325/1‑94 (MQ=60)
                                                                          CGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCA                     >  SRR4302540.3037671/1‑94 (MQ=60)
                                                                          CGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCA                     >  SRR4302540.34959/1‑94 (MQ=60)
                                                                          CGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCA                     >  SRR4302540.962265/1‑94 (MQ=60)
                                                                           GCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCAC                    >  SRR4302540.8249513/1‑94 (MQ=60)
                                                                           GCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCAC                    >  SRR4302540.4769359/1‑94 (MQ=60)
                                                                           GCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCAC                    <  SRR4302540.680528/94‑1 (MQ=60)
                                                                           GCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCAC                    <  SRR4302540.5783270/94‑1 (MQ=60)
                                                                            CCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACA                   <  SRR4302540.9143901/94‑1 (MQ=60)
                                                                            CCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACA                   <  SRR4302540.5508609/94‑1 (MQ=60)
                                                                            CCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACA                   <  SRR4302540.7076116/94‑1 (MQ=60)
                                                                             CGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAA                  >  SRR4302540.7002681/1‑94 (MQ=60)
                                                                              GTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAAC                 >  SRR4302540.7902278/1‑94 (MQ=60)
                                                                              GTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAAC                 >  SRR4302540.5851734/1‑94 (MQ=60)
                                                                              GTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAAC                 >  SRR4302540.1861178/1‑94 (MQ=60)
                                                                               TCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACC                >  SRR4302540.10141655/1‑94 (MQ=60)
                                                                                CACCCTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCT               <  SRR4302540.2782157/94‑1 (MQ=60)
                                                                                CACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCT               >  SRR4302540.1338703/1‑94 (MQ=60)
                                                                                 ACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTT              >  SRR4302540.9204256/1‑94 (MQ=60)
                                                                                  CACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTG             >  SRR4302540.486008/1‑94 (MQ=60)
                                                                                   ACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGC            >  SRR4302540.7816849/1‑94 (MQ=60)
                                                                                   ACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGC            <  SRR4302540.7336758/94‑1 (MQ=60)
                                                                                    CTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATACTAACGTTTTTATTCTCACAACCTTGCG           >  SRR4302540.5227148/1‑94 (MQ=60)
                                                                                     TCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATCATAACGTTTTTATTCTCACAACCTTCCGC          >  SRR4302540.7737175/1‑94 (MQ=60)
                                                                                     TCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGC          >  SRR4302540.7737174/1‑94 (MQ=60)
                                                                                      CTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCT         >  SRR4302540.8503524/1‑94 (MQ=60)
                                                                                       TTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTA        <  SRR4302540.734664/94‑1 (MQ=60)
                                                                                       TTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTA        >  SRR4302540.3852608/1‑94 (MQ=60)
                                                                                          GCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAAT     >  SRR4302540.1766098/1‑94 (MQ=60)
                                                                                          GCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAAT     <  SRR4302540.2726168/94‑1 (MQ=60)
                                                                                           CTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATG    >  SRR4302540.7574877/1‑94 (MQ=60)
                                                                                            TTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGA   <  SRR4302540.5931237/94‑1 (MQ=60)
                                                                                            TTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGA   >  SRR4302540.7500658/1‑94 (MQ=60)
                                                                                            TTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGA   >  SRR4302540.7500659/1‑94 (MQ=60)
                                                                                            TTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGA   >  SRR4302540.3836051/1‑94 (MQ=60)
                                                                                             TCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAA  <  SRR4302540.2002179/94‑1 (MQ=60)
                                                                                             |                                                                                             
ATTTCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAA  >  NC_000913/2170252‑2170438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑