Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,170,345 C→T G184E (GGA→GAA)  gatD ← galactitol‑1‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,170,3450CT100.0% 815.1 / NA 220G184E (GGA→GAA) gatDgalactitol‑1‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (100/120);  total (100/120)

Only 100 of 222 total aligned reads displayed.

CGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCT  >  NC_000913/2170256‑2170431
                                                                                         |                                                                                      
cGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCa                                                                                     <  2:4640739/93‑1 (MQ=255)
  cTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAgc                                                                                   >  2:1895602/1‑93 (MQ=255)
  cTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAgc                                                                                   <  1:896554/93‑1 (MQ=255)
   tACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAgcg                                                                                  >  2:3113917/1‑93 (MQ=255)
   tACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAgcg                                                                                  >  1:2416018/1‑93 (MQ=255)
   tACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAgcg                                                                                  >  2:5588981/1‑93 (MQ=255)
       gTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGAc                                                                              <  1:6159287/93‑1 (MQ=255)
        ttAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACa                                                                             <  1:3053815/93‑1 (MQ=255)
         tAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAg                                                                            <  1:1531039/93‑1 (MQ=255)
          aaaTGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAgc                                                                           <  1:3303324/93‑1 (MQ=255)
            aTGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAgcgc                                                                         >  2:1534747/1‑93 (MQ=255)
             tGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCa                                                                        >  1:2251966/1‑93 (MQ=255)
              gTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCAc                                                                       <  1:3795257/93‑1 (MQ=255)
              gTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCCTCCAGCGCGACAGCGCAc                                                                       <  1:5307442/93‑1 (MQ=255)
               tttGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACt                                                                      <  1:1718942/93‑1 (MQ=255)
               tttGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACt                                                                      >  2:646237/1‑93 (MQ=255)
                  gCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGaa                                                                   <  2:5940595/93‑1 (MQ=255)
                  gCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGaa                                                                   <  2:2553570/93‑1 (MQ=255)
                  gCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGaa                                                                   >  2:2073582/1‑93 (MQ=255)
                  gCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGaa                                                                   <  1:5852548/93‑1 (MQ=255)
                  gCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGaa                                                                   <  1:4269532/93‑1 (MQ=255)
                  gCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGaa                                                                   <  2:3921916/93‑1 (MQ=255)
                    aTCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATg                                                                 <  2:2378054/93‑1 (MQ=255)
                    aTCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATg                                                                 >  2:4200826/1‑93 (MQ=255)
                    aTCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATg                                                                 <  1:1894346/93‑1 (MQ=255)
                    aTCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATg                                                                 <  1:3837342/93‑1 (MQ=255)
                     tCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATgg                                                                >  1:3084758/1‑93 (MQ=255)
                     tCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATgg                                                                <  2:2163316/93‑1 (MQ=255)
                      cGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGc                                                               <  1:1136005/93‑1 (MQ=255)
                        cACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCca                                                             <  1:3554295/93‑1 (MQ=255)
                         aCCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCcag                                                            >  2:4724762/1‑93 (MQ=255)
                          ccGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCcagc                                                           >  2:1848290/1‑93 (MQ=255)
                           cGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCcagca                                                          >  2:795943/1‑93 (MQ=255)
                           cGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCcagca                                                          >  2:3809082/1‑93 (MQ=255)
                           cGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCcagca                                                          >  2:1614712/1‑93 (MQ=255)
                           cGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCcagca                                                          <  1:2608294/93‑1 (MQ=255)
                           cGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCcagca                                                          <  2:4766024/93‑1 (MQ=255)
                            gAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCcagcag                                                         <  1:3657325/93‑1 (MQ=255)
                             aaaGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAgg                                                        >  1:454031/1‑93 (MQ=255)
                             aaaGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAgg                                                        <  2:3640275/93‑1 (MQ=255)
                              aaGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGc                                                       <  1:2373352/93‑1 (MQ=255)
                              aaGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGc                                                       >  2:4252238/1‑93 (MQ=255)
                               aGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGcc                                                      >  2:1243971/1‑93 (MQ=255)
                               aGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGcc                                                      >  1:5571189/1‑93 (MQ=255)
                               aGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGcc                                                      <  1:959154/93‑1 (MQ=255)
                                gATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCa                                                     >  1:4721509/1‑93 (MQ=255)
                                  ttttGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAAt                                                   <  1:3152988/93‑1 (MQ=255)
                                     tgccagtgccagTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGt                                                >  2:3324574/1‑93 (MQ=255)
                                     tgccagtgccagTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGt                                                <  1:1180777/93‑1 (MQ=255)
                                       ccagtgccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTc                                              >  2:5066242/1‑93 (MQ=255)
                                       ccagtgccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTc                                              <  2:5164278/93‑1 (MQ=255)
                                         agtgccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCg                                            <  1:4398455/93‑1 (MQ=255)
                                          gtgccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCgg                                           >  2:6117826/1‑93 (MQ=255)
                                           tgccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGc                                          >  2:3171154/1‑93 (MQ=255)
                                           tgccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCAGGc                                          >  2:829263/1‑93 (MQ=255)
                                             ccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCAc                                        <  2:3663557/93‑1 (MQ=255)
                                              cagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCAcc                                       >  1:4142328/1‑93 (MQ=255)
                                               agtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCa                                      >  2:3799342/1‑93 (MQ=255)
                                                gtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCaa                                     <  1:6299989/93‑1 (MQ=255)
                                                 tttttCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCaat                                    >  1:5050736/1‑93 (MQ=255)
                                                 tttttCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCaat                                    <  1:5660724/93‑1 (MQ=255)
                                                   tttCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCaataa                                  <  1:2927677/93‑1 (MQ=255)
                                                   tttCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCaataa                                  <  1:1779360/93‑1 (MQ=255)
                                                   tttCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCaataa                                  <  1:5876230/93‑1 (MQ=255)
                                                   tttCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCaataa                                  <  1:5925410/93‑1 (MQ=255)
                                                   tttCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCaataa                                  <  1:676145/93‑1 (MQ=255)
                                                   tttCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCaataa                                  >  1:1224664/1‑93 (MQ=255)
                                                       tGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCaataataat                              >  2:3426451/1‑93 (MQ=255)
                                                        gAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAataataata                             >  2:4357469/1‑93 (MQ=255)
                                                          aCTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAAc                           <  2:2638271/93‑1 (MQ=255)
                                                          aCTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAAc                           <  1:2887971/93‑1 (MQ=255)
                                                          aCTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAAc                           <  2:5435646/93‑1 (MQ=255)
                                                           cTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACg                          <  2:2672435/93‑1 (MQ=255)
                                                           cTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACg                          <  1:4940548/93‑1 (MQ=255)
                                                           cTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACg                          >  1:4210428/1‑93 (MQ=255)
                                                            tAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGt                         <  1:1110665/93‑1 (MQ=255)
                                                               tGTCGATCGCCGTCACTCTCTTCGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGtttt                      <  2:2737526/93‑1 (MQ=255)
                                                               tGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGtttt                      <  1:794021/93‑1 (MQ=255)
                                                               tGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGtttt                      <  2:572310/93‑1 (MQ=255)
                                                               tGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGtttt                      <  1:1494066/93‑1 (MQ=255)
                                                               tGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGtttt                      >  2:3476787/1‑93 (MQ=255)
                                                                  cGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTAt                   >  2:106850/1‑93 (MQ=255)
                                                                   gATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTAtt                  >  1:2316453/1‑93 (MQ=255)
                                                                       gCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTca              <  1:1753201/93‑1 (MQ=255)
                                                                         cGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTcaca            >  2:72325/1‑93 (MQ=255)
                                                                         cGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTcaca            >  2:5259653/1‑93 (MQ=255)
                                                                          gTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACaa           >  2:4858721/1‑93 (MQ=255)
                                                                          gTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACaa           >  2:6239028/1‑93 (MQ=255)
                                                                           tCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAAc          <  1:691792/93‑1 (MQ=255)
                                                                             acacTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCt        >  1:391975/1‑93 (MQ=255)
                                                                             acacTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCt        >  1:1808975/1‑93 (MQ=255)
                                                                              cacTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCtt       >  1:39897/1‑93 (MQ=255)
                                                                              cacTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCtt       >  2:2332488/1‑93 (MQ=255)
                                                                              cacTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCtt       <  1:2809869/93‑1 (MQ=255)
                                                                               acTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACACCCTTg      >  2:3449618/1‑93 (MQ=255)
                                                                               acTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTg      >  1:1992415/1‑93 (MQ=255)
                                                                                ctctTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTgc     <  2:5770941/93‑1 (MQ=255)
                                                                                 tctTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTgcg    >  2:3765664/1‑93 (MQ=255)
                                                                                  ctTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTgcgc   >  1:1308104/1‑93 (MQ=255)
                                                                                   tttGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCt  >  1:1837026/1‑93 (MQ=255)
                                                                                         |                                                                                      
CGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCT  >  NC_000913/2170256‑2170431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAA  >  NC_000913/2170258‑2170438
                                                                                       |                                                                                             
CTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCG                                                                                         <  SRR4302533.901817/94‑1 (MQ=60)
CTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCG                                                                                         <  SRR4302533.2485226/94‑1 (MQ=60)
CTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCG                                                                                         >  SRR4302533.2430941/1‑94 (MQ=60)
 TACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGC                                                                                        >  SRR4302533.611583/1‑94 (MQ=60)
    TGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTCTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGAC                                                                                     >  SRR4302533.6342832/1‑94 (MQ=60)
     GTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACA                                                                                    <  SRR4302533.1798367/94‑1 (MQ=60)
     GTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACA                                                                                    >  SRR4302533.3168788/1‑94 (MQ=60)
     GTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACA                                                                                    <  SRR4302533.6196331/94‑1 (MQ=60)
      TTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAG                                                                                   >  SRR4302533.1377725/1‑94 (MQ=60)
      TTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAG                                                                                   <  SRR4302533.4641529/94‑1 (MQ=60)
      TTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAG                                                                                   <  SRR4302533.3072524/94‑1 (MQ=60)
       TAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGC                                                                                  <  SRR4302533.1540241/94‑1 (MQ=60)
        AAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCG                                                                                 <  SRR4302533.3323726/94‑1 (MQ=60)
        AAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCG                                                                                 >  SRR4302533.2286151/1‑94 (MQ=60)
          ATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCA                                                                               <  SRR4302533.4284041/94‑1 (MQ=60)
          ATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCA                                                                               >  SRR4302533.2265889/1‑94 (MQ=60)
           TGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCAC                                                                              <  SRR4302533.744644/94‑1 (MQ=60)
            GTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACT                                                                             <  SRR4302533.3818484/94‑1 (MQ=60)
            GTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCCTCCAGCGCGACAGCGCACT                                                                             <  SRR4302533.5339251/94‑1 (MQ=60)
             TTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTG                                                                            <  SRR4302533.1729366/94‑1 (MQ=60)
               TGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAA                                                                          <  SRR4302533.3926163/94‑1 (MQ=60)
                GCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAAT                                                                         <  SRR4302533.4295233/94‑1 (MQ=60)
                GCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAAT                                                                         <  SRR4302533.5887930/94‑1 (MQ=60)
                 CATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATG                                                                        <  SRR4302533.3334965/94‑1 (MQ=60)
                  ATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGG                                                                       >  SRR4302533.3103725/1‑94 (MQ=60)
                  ATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGG                                                                       <  SRR4302533.1905911/94‑1 (MQ=60)
                  ATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGG                                                                       <  SRR4302533.3860787/94‑1 (MQ=60)
                    CGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCC                                                                     <  SRR4302533.1142759/94‑1 (MQ=60)
                      CACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAG                                                                   <  SRR4302533.3576195/94‑1 (MQ=60)
                       ACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGC                                                                  >  SRR4302533.2956836/1‑94 (MQ=60)
                        CCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCA                                                                 >  SRR4302533.1192208/1‑94 (MQ=60)
                         CGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAG                                                                <  SRR4302533.2624311/94‑1 (MQ=60)
                         CGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAG                                                                >  SRR4302533.1838256/1‑94 (MQ=60)
                         CGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAG                                                                <  SRR4302533.435153/94‑1 (MQ=60)
                         CGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAG                                                                >  SRR4302533.5345955/1‑94 (MQ=60)
                          GAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGG                                                               <  SRR4302533.3679810/94‑1 (MQ=60)
                          GAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGG                                                               >  SRR4302533.456746/1‑94 (MQ=60)
                           AAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGC                                                              >  SRR4302533.3282948/1‑94 (MQ=60)
                            AAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCC                                                             <  SRR4302533.2388040/94‑1 (MQ=60)
                            AAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCC                                                             >  SRR4302533.5604862/1‑94 (MQ=60)
                             AGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCA                                                            >  SRR4302533.4750136/1‑94 (MQ=60)
                             AGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCA                                                            <  SRR4302533.964793/94‑1 (MQ=60)
                               ATTTTGCCAGTGCCGGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAAT                                                          <  SRR4302533.236588/94‑1 (MQ=60)
                               ATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAAT                                                          >  SRR4302533.5005145/1‑94 (MQ=60)
                                TTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATG                                                         <  SRR4302533.3581164/94‑1 (MQ=60)
                                TTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATG                                                         <  SRR4302533.3172370/94‑1 (MQ=60)
                                TTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATG                                                         <  SRR4302533.5902647/94‑1 (MQ=60)
                                TTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATG                                                         <  SRR4302533.2423687/94‑1 (MQ=60)
                                 TTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGG                                                        >  SRR4302533.3968211/1‑94 (MQ=60)
                                 TTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGG                                                        <  SRR4302533.2246247/94‑1 (MQ=60)
                                   TGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTT                                                      <  SRR4302533.1187870/94‑1 (MQ=60)
                                     CCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCC                                                    <  SRR4302533.3749601/94‑1 (MQ=60)
                                      CAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCG                                                   >  SRR4302533.2938042/1‑94 (MQ=60)
                                      CAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCG                                                   >  SRR4302533.489218/1‑94 (MQ=60)
                                      CAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCG                                                   <  SRR4302533.1731492/94‑1 (MQ=60)
                                      CAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCG                                                   <  SRR4302533.1604917/94‑1 (MQ=60)
                                      CAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCG                                                   <  SRR4302533.1383136/94‑1 (MQ=60)
                                       AGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGG                                                  <  SRR4302533.4425010/94‑1 (MQ=60)
                                         TGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCA                                                <  SRR4302533.5916034/94‑1 (MQ=60)
                                         TGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCA                                                <  SRR4302533.4199920/94‑1 (MQ=60)
                                           CCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACC                                              >  SRR4302533.4167444/1‑94 (MQ=60)
                                            CAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCA                                             >  SRR4302533.701422/1‑94 (MQ=60)
                                             AGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAA                                            >  SRR4302533.1876700/1‑94 (MQ=60)
                                             AGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAA                                            >  SRR4302533.5786361/1‑94 (MQ=60)
                                              GTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAAT                                           >  SRR4302533.1914388/1‑94 (MQ=60)
                                              GTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAAT                                           >  SRR4302533.5081280/1‑94 (MQ=60)
                                              GTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAAT                                           <  SRR4302533.2837193/94‑1 (MQ=60)
                                              GTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAAT                                           <  SRR4302533.6337605/94‑1 (MQ=60)
                                               TTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATA                                          <  SRR4302533.685902/94‑1 (MQ=60)
                                               TTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATA                                          <  SRR4302533.5694926/94‑1 (MQ=60)
                                                TTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAA                                         >  SRR4302533.2005133/1‑94 (MQ=60)
                                                TTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAA                                         >  SRR4302533.1232005/1‑94 (MQ=60)
                                                TTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAA                                         <  SRR4302533.1782332/94‑1 (MQ=60)
                                                TTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAA                                         <  SRR4302533.411622/94‑1 (MQ=60)
                                                 TTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAAT                                        <  SRR4302533.680140/94‑1 (MQ=60)
                                                 TTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAAT                                        <  SRR4302533.5911755/94‑1 (MQ=60)
                                                 TTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAAT                                        <  SRR4302533.2945590/94‑1 (MQ=60)
                                                 TTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAAT                                        <  SRR4302533.5961232/94‑1 (MQ=60)
                                                 TTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAAT                                        <  SRR4302533.1790195/94‑1 (MQ=60)
                                                 TTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAAT                                        <  SRR4302533.279487/94‑1 (MQ=60)
                                                   TCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAA                                      <  SRR4302533.3858199/94‑1 (MQ=60)
                                                    CTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAAT                                     >  SRR4302533.3102836/1‑94 (MQ=60)
                                                       AACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAAC                                  <  SRR4302533.26307/94‑1 (MQ=60)
                                                       AACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAAC                                  <  SRR4302533.3721410/94‑1 (MQ=60)
                                                       AACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAAC                                  >  SRR4302533.1512158/1‑94 (MQ=60)
                                                        ACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACG                                 <  SRR4302533.2905724/94‑1 (MQ=60)
                                                        ACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACG                                 >  SRR4302533.4235896/1‑94 (MQ=60)
                                                        ACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACG                                 <  SRR4302533.180922/94‑1 (MQ=60)
                                                         CTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGT                                <  SRR4302533.4970456/94‑1 (MQ=60)
                                                          TAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTT                               <  SRR4302533.1117254/94‑1 (MQ=60)
                                                           AATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTT                              >  SRR4302533.1157960/1‑94 (MQ=60)
                                                            ATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTT                             <  SRR4302533.6239414/94‑1 (MQ=60)
                                                            ATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTT                             <  SRR4302533.1139818/94‑1 (MQ=60)
                                                             TGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTT                            <  SRR4302533.798692/94‑1 (MQ=60)
                                                             TGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTT                            <  SRR4302533.1503072/94‑1 (MQ=60)
                                                              GTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTA                           >  SRR4302533.5580642/1‑94 (MQ=60)
                                                               TCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTAT                          <  SRR4302533.5202693/94‑1 (MQ=60)
                                                                CGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGTCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATT                         <  SRR4302533.4073667/94‑1 (MQ=60)
                                                                CGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATT                         >  SRR4302533.2330795/1‑94 (MQ=60)
                                                                CGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATT                         >  SRR4302533.3078658/1‑94 (MQ=60)
                                                                 GATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTC                        <  SRR4302533.5623833/94‑1 (MQ=60)
                                                                  ATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCT                       <  SRR4302533.4438139/94‑1 (MQ=60)
                                                                    CGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCA                     <  SRR4302533.6092240/94‑1 (MQ=60)
                                                                     GCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCAC                    <  SRR4302533.1763879/94‑1 (MQ=60)
                                                                     CCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCAC                    <  SRR4302533.4571170/94‑1 (MQ=60)
                                                                      CCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACA                   <  SRR4302533.1241104/94‑1 (MQ=60)
                                                                      CCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACA                   >  SRR4302533.836138/1‑94 (MQ=60)
                                                                         TCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACC                <  SRR4302533.695879/94‑1 (MQ=60)
                                                                          CACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCT               >  SRR4302533.394349/1‑94 (MQ=60)
                                                                          CACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCT               >  SRR4302533.1819981/1‑94 (MQ=60)
                                                                           ACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTT              >  SRR4302533.40163/1‑94 (MQ=60)
                                                                            CACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTG             >  SRR4302533.2004629/1‑94 (MQ=60)
                                                                            CACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTG             <  SRR4302533.2827160/94‑1 (MQ=60)
                                                                              CTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCG           <  SRR4302533.913543/94‑1 (MQ=60)
                                                                               TCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGC          >  SRR4302533.1315958/1‑94 (MQ=60)
                                                                                CTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCT         >  SRR4302533.1848229/1‑94 (MQ=60)
                                                                                     CTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATACCGTTTTTATTCTCACAACCTTGCGCTAAATG    <  SRR4302533.5528233/94‑1 (MQ=60)
                                                                                     CTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATG    >  SRR4302533.2812650/1‑94 (MQ=60)
                                                                                     CTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATG    >  SRR4302533.6319025/1‑94 (MQ=60)
                                                                                      TTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGA   >  SRR4302533.3243557/1‑94 (MQ=60)
                                                                                       TCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAA  <  SRR4302533.3069721/94‑1 (MQ=60)
                                                                                       TCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCAGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAA  >  SRR4302533.5892946/1‑94 (MQ=60)
                                                                                       |                                                                                             
CTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAA  >  NC_000913/2170258‑2170438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑