Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,207,023	A→G	D346G (GAC→GGC)	yehP →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,207,023	0	A	G	99.5%	658.6 / ‑5.1	208	D346G (GAC→GGC)	yehP	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (93/114); total (94/114)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

Only 100 of 214 total aligned reads displayed.

TACTGACGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGACCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTGCGGAGAATC  >  NC_000913/2206933‑2207112

tACTGACGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGcc                                                                                         <  2:437147/93‑1 (MQ=255)

  cTGACGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCcgc                                                                                       >  1:10002704/1‑93 (MQ=255)

  cTGACGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCcgc                                                                                       <  2:5893677/93‑1 (MQ=255)

    gACGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGTCTGGCAGCGCTCGATAGCACCGCAGCACCTTGCTATGGCCGCGa                                                                                     >  2:3678093/1‑93 (MQ=255)

    gACGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGa                                                                                     <  1:7243241/93‑1 (MQ=255)

      cGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATa                                                                                   <  1:3314257/93‑1 (MQ=255)

      cGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATa                                                                                   <  2:4490006/93‑1 (MQ=255)

      cGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGACCGCGATa                                                                                   >  1:4403956/1‑93 (MQ=255)

       gCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATAc                                                                                  >  2:4965558/1‑93 (MQ=255)

       gCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATAc                                                                                  <  2:5285079/93‑1 (MQ=255)

        cATCAGGTGAAAAAGTGTGTCCAGAGCGGCATTAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACg                                                                                 >  1:9475464/1‑93 (MQ=255)

        cATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACg                                                                                 >  1:5440220/1‑93 (MQ=255)

           cAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGAGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGcc                                                                              >  2:9727961/1‑93 (MQ=255)

            aGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGccc                                                                             <  2:1089436/93‑1 (MQ=255)

            aGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGccc                                                                             <  2:1850149/93‑1 (MQ=255)

            aGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGccc                                                                             <  2:6991214/93‑1 (MQ=255)

            aGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGccc                                                                             <  1:6630221/93‑1 (MQ=255)

             ggTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCa                                                                            <  2:3446285/93‑1 (MQ=255)

              gTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAg                                                                           <  2:7252668/93‑1 (MQ=255)

               tGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAgg                                                                          <  2:3113989/93‑1 (MQ=255)

               tGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAgg                                                                          <  2:2056189/93‑1 (MQ=255)

                 aaaaaGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGgcg                                                                        >  1:6493086/1‑93 (MQ=255)

                  aaaaGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGgcgc                                                                       >  2:8068204/1‑93 (MQ=255)

                   aaaGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCt                                                                      >  1:5210514/1‑93 (MQ=255)

                   aaaGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCt                                                                      >  2:5444636/1‑93 (MQ=255)

                    aaGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTg                                                                     <  1:2743645/93‑1 (MQ=255)

                     aGTGTGTGCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTgg                                                                    <  1:8312053/93‑1 (MQ=255)

                       tgtgtCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGtt                                                                  >  1:4760061/1‑93 (MQ=255)

                       tgtgtCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGC‑ACACCTTGCGATGGGCGCGGTACGGGCCAGGCGCTTGTTa                                                                 >  2:6002465/1‑93 (MQ=255)

                         tgtCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTaa                                                                <  1:5931098/93‑1 (MQ=255)

                            ccAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGt                                                             <  1:9971115/93‑1 (MQ=255)

                             cAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTc                                                            >  1:1457754/1‑93 (MQ=255)

                             cAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTc                                                            <  1:3810041/93‑1 (MQ=255)

                              agagCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCg                                                           <  2:6416673/93‑1 (MQ=255)

                               gagCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCATCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCgg                                                          >  2:4892650/1‑93 (MQ=255)

                               gagCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCgg                                                          >  2:3283721/1‑93 (MQ=255)

                                agCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGgc                                                         <  1:7602566/93‑1 (MQ=255)

                                 gCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATCCGGCCCAGGCGCTGGTTAATGTCGgcg                                                        >  2:2583087/1‑93 (MQ=255)

                                 gCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGgcg                                                        >  2:336584/1‑93 (MQ=255)

                                 gCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGgcg                                                        <  2:3987001/93‑1 (MQ=255)

                                  cGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGgcgc                                                       <  2:9713068/93‑1 (MQ=255)

                                   ggCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGca                                                      <  1:7162620/93‑1 (MQ=255)

                                    gCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGcac                                                     >  1:9042180/1‑93 (MQ=255)

                                      aTCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACaa                                                   <  2:9024551/93‑1 (MQ=255)

                                       tCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACaaa                                                  >  1:1410993/1‑93 (MQ=255)

                                       tCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACaaa                                                  >  2:3007850/1‑93 (MQ=255)

                                       tCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACaaa                                                  >  1:926640/1‑93 (MQ=255)

                                        cAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAAt                                                 <  2:8721439/93‑1 (MQ=255)

                                         aaaGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATa                                                <  1:4198135/93‑1 (MQ=255)

                                          aaGTGCTGGGATTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAg                                               >  2:8896564/1‑93 (MQ=255)

                                           aGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAgc                                              <  2:9110147/93‑1 (MQ=255)

                                            gTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAgcc                                             <  2:8500873/93‑1 (MQ=255)

                                              gCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAgccgc                                           <  2:6762307/93‑1 (MQ=255)

                                               cTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAgccgcc                                          <  1:4523814/93‑1 (MQ=255)

                                                tGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCa                                         <  1:4007643/93‑1 (MQ=255)

                                                 gggACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCAt                                        >  1:3237586/1‑93 (MQ=255)

                                                   gACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGa                                      <  1:9729314/93‑1 (MQ=255)

                                                   gACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGa                                      <  2:7706266/93‑1 (MQ=255)

                                                   gACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGa                                      <  1:3904545/93‑1 (MQ=255)

                                                    aCTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGAc                                     <  1:1385370/93‑1 (MQ=255)

                                                    aCTGGCAGCGCTCGATACCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGAc                                     <  2:2281519/93‑1 (MQ=255)

                                                      tGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGc                                   <  2:9586750/93‑1 (MQ=255)

                                                      tGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGc                                   <  2:1660655/93‑1 (MQ=255)

                                                      tGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGc                                   >  1:5098063/1‑93 (MQ=255)

                                                       ggCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACACATAGCCGCCATGACGcc                                  >  1:9819327/1‑93 (MQ=255)

                                                        gCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCg                                 >  2:2860625/1‑93 (MQ=255)

                                                        gCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCg                                 >  1:3967183/1‑93 (MQ=255)

                                                        gCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCg                                 <  1:6688358/93‑1 (MQ=255)

                                                         cAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCTCCATGACGCCgg                                >  2:2975946/1‑93 (MQ=255)

                                                         cAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCgg                                <  2:7375320/93‑1 (MQ=255)

                                                           gcgcTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCgggg                              >  2:4872670/1‑92 (MQ=255)

                                                           gcgcTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGc                              >  1:3706984/1‑93 (MQ=255)

                                                            cgcTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGACATGACGCAGGGCg                             >  2:2344404/1‑93 (MQ=255)

                                                               tCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGTCGGGCGAGc                          <  2:6272746/93‑1 (MQ=255)

                                                                 gATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTg                        <  1:5754069/93‑1 (MQ=255)

                                                                  aTAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTgg                       >  1:1922518/1‑93 (MQ=255)

                                                                  aTAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTgg                       <  1:5869595/93‑1 (MQ=255)

                                                                   tAGCACCGCAACCCCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGc                      <  1:8691559/93‑1 (MQ=255)

                                                                   tAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGc                      >  1:629860/1‑93 (MQ=255)

                                                                    aGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGca                     <  1:2304675/93‑1 (MQ=255)

                                                                     gCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGcat                    <  2:146843/93‑1 (MQ=255)

                                                                       aCCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGcatca                  >  1:6103054/1‑93 (MQ=255)

                                                                        ccGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGcatcat                 >  1:806695/1‑93 (MQ=255)

                                                                        ccGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGcatcat                 <  1:4862987/93‑1 (MQ=255)

                                                                        ccGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGcatcat                 >  1:6151093/1‑93 (MQ=255)

                                                                          gCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATgg               <  2:7056075/93‑1 (MQ=255)

                                                                          gCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATgg               >  1:8054744/1‑93 (MQ=255)

                                                                           cAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGc              >  2:6649310/1‑93 (MQ=255)

                                                                            aaCACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCt             >  2:4791617/1‑93 (MQ=255)

                                                                            aaCACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCt             <  2:2392801/93‑1 (MQ=255)

                                                                            aaCACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCt             >  2:5341919/1‑93 (MQ=255)

                                                                               cccTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTGc          <  1:9539271/92‑1 (MQ=255)

                                                                               cccTTGCTAGGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTGc          <  2:6392625/92‑1 (MQ=255)

                                                                               acCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGCTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTGc          <  2:4688418/93‑1 (MQ=255)

                                                                                ccTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCttttg         >  2:6502457/1‑90 (MQ=255)

                                                                                ccTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCttttg         >  1:7715614/1‑90 (MQ=255)

                                                                                 cTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCttttgg        >  1:8955237/1‑89 (MQ=255)

                                                                                    gCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTTCGggga     >  2:4119250/1‑93 (MQ=255)

                                                                                    gCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTGCGggga     >  1:1834583/1‑93 (MQ=255)

                                                                                     cTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTGCGGAGaa    <  1:9743387/93‑1 (MQ=255)

TACTGACGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGACCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTGCGGAGAATC  >  NC_000913/2206933‑2207112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CATTACTGACGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGACCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTGCGGAGAATCTTCA  >  NC_000913/2206930‑2207116

CATTACTGACGCATCAGGTGAAAAAGTGTGTCCAGAGCGTCATCAAAGTGCTGGGACTGTCAGCGCTCGATAGCACCGCAACACCTTGCTATGG                                                                                               <  SRR4302540.1428321/94‑1 (MQ=60)

CATTACTGACGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGG                                                                                               <  SRR4302540.1903753/94‑1 (MQ=60)

CATTACTGACGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGG                                                                                               >  SRR4302540.6463788/1‑94 (MQ=60)

 ATTACTGACGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGC                                                                                              >  SRR4302540.62815/1‑94 (MQ=60)

    ACTGACGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGC                                                                                           <  SRR4302540.2828915/94‑1 (MQ=60)

    ACTGACGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGC                                                                                           >  SRR4302540.10119222/1‑94 (MQ=60)

    ACTGACGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGC                                                                                           <  SRR4302540.814513/94‑1 (MQ=60)

      TGACGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGA                                                                                         >  SRR4302540.675595/1‑94 (MQ=60)

       GACGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGAT                                                                                        <  SRR4302540.7324040/94‑1 (MQ=60)

        ACGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATA                                                                                       >  SRR4302540.8655450/1‑94 (MQ=60)

        ACGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGACCGCGATA                                                                                       >  SRR4302540.4451707/1‑94 (MQ=60)

         CGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATAC                                                                                      >  SRR4302540.5878026/1‑94 (MQ=60)

         CGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATAC                                                                                      <  SRR4302540.3350583/94‑1 (MQ=60)

         CGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATAC                                                                                      <  SRR4302540.6994993/94‑1 (MQ=60)

          GCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATTAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACG                                                                                     >  SRR4302540.9587840/1‑94 (MQ=60)

          GCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCCCCGCAACACCTTGCTATGGCCGCGCTACG                                                                                     >  SRR4302540.9587841/1‑94 (MQ=60)

          GCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACG                                                                                     >  SRR4302540.5500074/1‑94 (MQ=60)

          GCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACG                                                                                     >  SRR4302540.445325/1‑94 (MQ=60)

           CATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGG                                                                                    <  SRR4302540.10086542/94‑1 (MQ=60)

           CATCAGGTGAAAAAGTGTGTCCAGAGCCGCATCAAAGTGCTGGGACTGCCAGCGCTCGATAGCACCGCAACCCCTTGCTATGGCCGCGATACGG                                                                                    <  SRR4302540.1977667/94‑1 (MQ=60)

            ATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGC                                                                                   <  SRR4302540.3982241/94‑1 (MQ=60)

              CAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCC                                                                                 <  SRR4302540.7301061/94‑1 (MQ=60)

              CAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCC                                                                                 <  SRR4302540.6647760/94‑1 (MQ=60)

               AGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCA                                                                                <  SRR4302540.6703615/94‑1 (MQ=60)

               AGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCA                                                                                <  SRR4302540.4176264/94‑1 (MQ=60)

               AGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCA                                                                                <  SRR4302540.3890884/94‑1 (MQ=60)

                GGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAG                                                                               <  SRR4302540.5942393/94‑1 (MQ=60)

                 GTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGG                                                                              <  SRR4302540.7785854/94‑1 (MQ=60)

                   GAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCG                                                                            >  SRR4302540.6565296/1‑94 (MQ=60)

                   GAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCG                                                                            >  SRR4302540.6565295/1‑94 (MQ=60)

                   GAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCG                                                                            >  SRR4302540.9575268/1‑94 (MQ=60)

                     AAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCT                                                                          >  SRR4302540.5267282/1‑94 (MQ=60)

                      AAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTG                                                                         >  SRR4302540.1812675/1‑94 (MQ=60)

                       AAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGG                                                                        <  SRR4302540.2773271/94‑1 (MQ=60)

                        TGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGT                                                                       >  SRR4302540.4413131/1‑94 (MQ=60)

                        AGTGTGTGCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGT                                                                       <  SRR4302540.8408543/94‑1 (MQ=60)

                         GTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTT                                                                      >  SRR4302540.4810857/1‑94 (MQ=60)

                         GTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTT                                                                      <  SRR4302540.9845639/94‑1 (MQ=60)

                           GTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAA                                                                    <  SRR4302540.1407066/94‑1 (MQ=60)

                            TGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAAT                                                                   <  SRR4302540.5996637/94‑1 (MQ=60)

                            TGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAAT                                                                   <  SRR4302540.6216126/94‑1 (MQ=60)

                              TCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGT                                                                 <  SRR4302540.3486763/94‑1 (MQ=60)

                               CCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTC                                                                <  SRR4302540.10087429/94‑1 (MQ=60)

                               CCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTC                                                                >  SRR4302540.1473615/1‑94 (MQ=60)

                                CAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCG                                                               <  SRR4302540.9917481/94‑1 (MQ=60)

                                CAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCG                                                               >  SRR4302540.9801153/1‑94 (MQ=60)

                                CAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCG                                                               <  SRR4302540.3851535/94‑1 (MQ=60)

                                  GAGCGGCATCAAAGTGCTGGGACTGGCAGTGTTCGACAGCACCGCAACACCTTGCTATGGCCGCGACACGGCCCAGGCGCTGGTTAATGTCAGC                                                             >  SRR4302540.3960584/1‑94 (MQ=60)

                                   AGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCG                                                            <  SRR4302540.7687986/94‑1 (MQ=60)

                                      GGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCAC                                                         <  SRR4302540.7242471/94‑1 (MQ=60)

                                      GGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCAC                                                         >  SRR4302540.835171/1‑94 (MQ=60)

                                      GGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCAC                                                         >  SRR4302540.9150721/1‑94 (MQ=60)

                                       GCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACA                                                        <  SRR4302540.782835/94‑1 (MQ=60)

                                         ATCAAAGTGCTGGGACTGGTAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCACCGGCGCTGGTTAATGTCGGCGCACAAA                                                      >  SRR4302540.6118778/1‑94 (MQ=60)

                                         ATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAA                                                      >  SRR4302540.936397/1‑94 (MQ=60)

                                         ATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAA                                                      >  SRR4302540.1426203/1‑94 (MQ=60)

                                            AAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCTGCGCCCAAATAG                                                   >  SRR4302540.8954124/1‑94 (MQ=60)

                                            AAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAG                                                   <  SRR4302540.4243812/94‑1 (MQ=60)

                                             AAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGC                                                  <  SRR4302540.2720299/94‑1 (MQ=60)

                                              AGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCC                                                 >  SRR4302540.4996442/1‑94 (MQ=60)

                                                  CTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCA                                             <  SRR4302540.4572803/94‑1 (MQ=60)

                                                   TGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCAT                                            >  SRR4302540.3272804/1‑94 (MQ=60)

                                                   TGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCAT                                            <  SRR4302540.10100604/94‑1 (MQ=60)

                                                   TGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCAT                                            >  SRR4302540.5533985/1‑94 (MQ=60)

                                                   TGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCAT                                            <  SRR4302540.4050791/94‑1 (MQ=60)

                                                      GACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGAC                                         <  SRR4302540.3946888/94‑1 (MQ=60)

                                                      GACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGAC                                         >  SRR4302540.6464945/1‑94 (MQ=60)

                                                      GACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGAC                                         <  SRR4302540.9843569/94‑1 (MQ=60)

                                                      GACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGAC                                         <  SRR4302540.6222871/94‑1 (MQ=60)

                                                       ACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACG                                        <  SRR4302540.1400176/94‑1 (MQ=60)

                                                       ACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACG                                        <  SRR4302540.5410445/94‑1 (MQ=60)

                                                       ACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACG                                        <  SRR4302540.916582/94‑1 (MQ=60)

                                                        CTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGC                                       >  SRR4302540.5153233/1‑94 (MQ=60)

                                                         TGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACACATAGCCGCCATGACGCC                                      >  SRR4302540.9934220/1‑94 (MQ=60)

                                                         TGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCC                                      <  SRR4302540.9541173/94‑1 (MQ=60)

                                                          GGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCG                                     >  SRR4302540.4009951/1‑94 (MQ=60)

                                                          GGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCG                                     >  SRR4302540.4009950/1‑94 (MQ=60)

                                                           GCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGG                                    <  SRR4302540.7860080/94‑1 (MQ=60)

                                                           GCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGG                                    <  SRR4302540.6762206/94‑1 (MQ=60)

                                                             AGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGC                                  >  SRR4302540.9130183/1‑94 (MQ=60)

                                                             AGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGC                                  >  SRR4302540.3747443/1‑94 (MQ=60)

                                                                GCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAG                               <  SRR4302540.5207406/94‑1 (MQ=60)

                                                                 CTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGC                              >  SRR4302540.4751029/1‑94 (MQ=60)

                                                                  CCGATAGCCCCGCAACCCCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCT                             <  SRR4302540.1238995/94‑1 (MQ=60)

                                                                    GATAGCACCGCACCACCTGGTTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGG                           <  SRR4302540.755523/94‑1 (MQ=60)

                                                                    GATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGG                           <  SRR4302540.5817582/94‑1 (MQ=60)

                                                                    GATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGG                           >  SRR4302540.1943479/1‑94 (MQ=60)

                                                                     ATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCGCAAATAGCCGCCATGACGCCGGGCGAGCTGGC                          >  SRR4302540.1456227/1‑94 (MQ=60)

                                                                     ATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGC                          <  SRR4302540.5934476/94‑1 (MQ=60)

                                                                     ATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGC                          >  SRR4302540.636560/1‑94 (MQ=60)

                                                                      TAGCACCGCAACCCCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCA                         <  SRR4302540.8792466/94‑1 (MQ=60)

                                                                       AGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCAT                        <  SRR4302540.2329474/94‑1 (MQ=60)

                                                                         CACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCA                      >  SRR4302540.6171015/1‑94 (MQ=60)

                                                                          ACCGCAACCCCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCAT                     <  SRR4302540.3127674/94‑1 (MQ=60)

                                                                          ACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCAT                     >  SRR4302540.815265/1‑94 (MQ=60)

                                                                          ACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCAT                     >  SRR4302540.6219602/1‑94 (MQ=60)

                                                                           CCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATG                    <  SRR4302540.4915137/94‑1 (MQ=60)

                                                                           CCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATG                    <  SRR4302540.3780950/94‑1 (MQ=60)

                                                                            CGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGG                   >  SRR4302540.8148281/1‑94 (MQ=60)

                                                                              CAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCT                 <  SRR4302540.1929886/94‑1 (MQ=60)

                                                                                 CACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTGC              <  SRR4302540.4223491/94‑1 (MQ=60)

                                                                                  CCCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTGCG             <  SRR4302540.9652163/94‑1 (MQ=60)

                                                                                  ACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTttg             >  SRR4302540.7804651/1‑91 (MQ=60)

                                                                                   CCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTttgg            >  SRR4302540.9060972/1‑90 (MQ=60)

                                                                                   CCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTTCGG            >  SRR4302540.4844516/1‑94 (MQ=60)

                                                                                   CCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTGCGG            <  SRR4302540.5488098/94‑1 (MQ=60)

                                                                                     TTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTttgggg          >  SRR4302540.8198825/1‑88 (MQ=60)

                                                                                      TGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTGCGGGGA         >  SRR4302540.1854660/1‑94 (MQ=60)

                                                                                        CTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTttggggaaa       >  SRR4302540.4257686/1‑85 (MQ=60)

                                                                                        CTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTGCGGAGAAT       <  SRR4302540.9857742/94‑1 (MQ=60)

                                                                                          ATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTtccgaggatat     >  SRR4302540.3698962/1‑83 (MQ=60)

                                                                                          ATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTGCGGAGGATCT     >  SRR4302540.8257074/1‑94 (MQ=60)

                                                                                          ATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTGCGGAGAATCT     <  SRR4302540.3337594/94‑1 (MQ=60)

                                                                                           TGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTGCGGAGAATCTT    <  SRR4302540.6039627/94‑1 (MQ=60)

                                                                                           TGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTGCGGAGAATCTT    <  SRR4302540.7836295/94‑1 (MQ=60)

                                                                                           TGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTGCGGAGAATCTT    <  SRR4302540.659839/94‑1 (MQ=60)

                                                                                            GGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTtcggcggaacatt   >  SRR4302540.9646478/1‑81 (MQ=60)

                                                                                             GCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTttcggggacttttc  >  SRR4302540.4300860/1‑80 (MQ=60)

                                                                                             GCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTGtggggaatttttc  >  SRR4302540.7726958/1‑81 (MQ=60)

                                                                                             GCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTGCGGAGAATCTTCA  <  SRR4302540.6556847/94‑1 (MQ=60)

                                                                                             GCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTGCGGAGAATCTTCA  <  SRR4302540.2980051/94‑1 (MQ=60)

                                                                                             GCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTGCGGAGAATCTTCA  <  SRR4302540.6370791/94‑1 (MQ=60)

                                                                                             GCCGCCATACGGCCCCGGCGCTGGGTAATGGCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTGCGGAGAATCTTCA  <  SRR4302540.2980050/94‑1 (MQ=60)

CATTACTGACGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGACCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTGCGGAGAATCTTCA  >  NC_000913/2206930‑2207116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`