Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,208,505 G→A R462R (CGG→CGA)  b2122 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,208,5050GA99.2% 815.4 / ‑5.6 236R462R (CGG→CGA) b2122hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (122/111);  total (123/113)
Fisher's exact test for biased strand distribution p-value = 2.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.59e-01

Only 100 of 247 total aligned reads displayed.

TGCCTGGCAAATGTTGAGCGCG‑CCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGGCCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATGC  >  NC_000913/2208415‑2208594
                                                                                           |                                                                                         
tGCCTGGCAAATGTTGAGCGCG‑CCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGAcc                                                                                         >  1:3888832/1‑93 (MQ=255)
   cTGGCAAATGTTGAGCGCG‑CCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACctct                                                                                      >  2:5683688/1‑93 (MQ=255)
   cTGGCAAATGTTGAGCGCG‑CCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACcgct                                                                                      <  2:8116953/93‑1 (MQ=255)
     gtcAAATGTTGA‑CGCGCCCGTTAAGCGCGCCGGCGATTCTGGCTTTGCGGGAATGTTTGCCACAGAGTCCAACCGCCTGCATACGCCCTCTTa                                                                                    <  2:5408395/91‑1 (MQ=255)
       cAAATGTTGAGCGCG‑CCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAAt                                                                                  >  1:6027888/1‑93 (MQ=255)
        aaaTGTTGAGCGCG‑CCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCTGCGTCCCCCCTCCTTCCTACCCCCTCTCAATc                                                                                 >  1:9692406/1‑93 (MQ=255)
          aTGTTGAGCGCG‑CCGTTACGCCATCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAg                                                                               <  2:7219638/93‑1 (MQ=255)
           tGTTGAGCGCG‑CCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAgg                                                                              >  2:7435673/1‑93 (MQ=255)
            gTTGAGCGCG‑CGGTTACGCCAGCCGGGCATTGTGGCTTGGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGt                                                                             <  1:4604833/93‑1 (MQ=255)
            gTTGAGCGCG‑CCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGt                                                                             <  2:2365436/93‑1 (MQ=255)
            gTTGAGCGCG‑CCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGt                                                                             >  1:5535473/1‑93 (MQ=255)
             ttGAGCGCG‑CCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTc                                                                            <  2:9190735/93‑1 (MQ=255)
             ttGAGCGCG‑CCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTc                                                                            >  2:3755507/1‑93 (MQ=255)
               gAGCGCG‑CCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGa                                                                          >  1:7573350/1‑93 (MQ=255)
                   gcg‑cCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAAc                                                                      >  1:6844657/1‑93 (MQ=255)
                    cg‑cCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACt                                                                     >  2:2213208/1‑93 (MQ=255)
                    cg‑cCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACt                                                                     >  1:162046/1‑93 (MQ=255)
                    cg‑cCGTTACGCCAGCCGGGCATTGTGGCTTGGCGTTATTATTTACGCCGGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACt                                                                     <  2:5451059/93‑1 (MQ=255)
                     g‑cCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACtt                                                                    <  1:3805043/93‑1 (MQ=255)
                     g‑cCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACtt                                                                    <  1:3856564/93‑1 (MQ=255)
                       ccGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACttt                                                                   >  2:8620936/1‑92 (MQ=255)
                       ccGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACtta                                                                   >  1:9916717/1‑93 (MQ=255)
                         gTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACttatt                                                                 >  2:6501945/1‑93 (MQ=255)
                         gTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACttatt                                                                 <  1:934382/93‑1 (MQ=255)
                         gTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACttatt                                                                 <  2:2385077/93‑1 (MQ=255)
                         gTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCCTAATCAGGTCGATAACttatt                                                                 <  1:3094386/93‑1 (MQ=255)
                          ttACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACttattt                                                                >  1:3486075/1‑93 (MQ=255)
                          ttACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACttattt                                                                <  1:8777470/93‑1 (MQ=255)
                          ttACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACttattt                                                                >  1:7732856/1‑93 (MQ=255)
                          ttACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACttattt                                                                <  2:8780187/93‑1 (MQ=255)
                           tACGCCAGCCGGGCATTGTGGCTTTGCGTGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACttattta                                                               <  2:957062/93‑1 (MQ=255)
                           tACGCCAGCCGGGCATTGTGGCTTTGCGGGAATCTTTACGCCAGCGTCCCCCCGCCTGCATACGCCCTCTTAATCAGGTCGATAACttattta                                                               >  1:7282819/1‑93 (MQ=255)
                           tACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACttattta                                                               <  2:5226110/93‑1 (MQ=255)
                            aCGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACttatttat                                                              <  2:6015247/93‑1 (MQ=255)
                            aCGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGAGAACttatttat                                                              >  1:6642088/1‑93 (MQ=255)
                            aCGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGAATACGACCTCTTAATCAGGTCGATAACttatttat                                                              <  1:1791302/93‑1 (MQ=255)
                             cGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTtatttatt                                                             <  1:6525033/93‑1 (MQ=255)
                              gCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTc                                                            <  1:4751615/93‑1 (MQ=255)
                              gCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTc                                                            <  2:2738901/93‑1 (MQ=255)
                              gCCAGCCGGGCATTGTGGCTGTGCGGGAATATTTACTCCAGCGACCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTc                                                            <  2:6780667/93‑1 (MQ=255)
                               ccAGCCGGGCATTGTGGCTTGGCGGGAATATTTACGCCAGCGCCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCt                                                           <  2:7544260/93‑1 (MQ=255)
                                 aGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGc                                                         >  2:4545353/1‑93 (MQ=255)
                                 aGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGc                                                         >  2:9679595/1‑93 (MQ=255)
                                 aGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGc                                                         <  1:4401546/93‑1 (MQ=255)
                                  gCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGcc                                                        >  1:6270866/1‑93 (MQ=255)
                                    cGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCgg                                                      <  1:3760059/93‑1 (MQ=255)
                                       gCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATTCGGCCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCg                                                   <  2:2850370/93‑1 (MQ=255)
                                          ttGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCTGGTCGCTg                                                >  1:9450740/1‑93 (MQ=255)
                                          ttGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTg                                                >  1:7127992/1‑93 (MQ=255)
                                          ttGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTg                                                >  1:9404593/1‑93 (MQ=255)
                                          ttGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTg                                                >  1:5706215/1‑93 (MQ=255)
                                          ttGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTg                                                >  1:5229027/1‑93 (MQ=255)
                                          ttGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTg                                                >  1:1460450/1‑93 (MQ=255)
                                          ttGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTg                                                >  1:302117/1‑93 (MQ=255)
                                           tgtgGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGa                                               <  2:1121968/93‑1 (MQ=255)
                                            gtgGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAg                                              >  2:2600693/1‑93 (MQ=255)
                                                 tttGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTAt                                         <  2:4687307/93‑1 (MQ=255)
                                                 tttGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTAt                                         >  2:3079848/1‑93 (MQ=255)
                                                    gCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTAGCTGAGTGTATTTc                                      >  1:4423519/1‑93 (MQ=255)
                                                      gggAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGc                                    >  1:109678/1‑93 (MQ=255)
                                                        gAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTc                                  <  2:1516499/93‑1 (MQ=255)
                                                        gAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTc                                  >  1:5381783/1‑93 (MQ=255)
                                                         aaTATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCg                                 >  1:2176958/1‑93 (MQ=255)
                                                           tatTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGt                               >  2:729598/1‑93 (MQ=255)
                                                           tatTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGt                               >  1:2662223/1‑93 (MQ=255)
                                                           tatTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGt                               <  2:5668922/93‑1 (MQ=255)
                                                            atTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGtt                              <  2:4189397/93‑1 (MQ=255)
                                                            atTTACGCCAGCGGCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGtt                              <  2:1792507/93‑1 (MQ=255)
                                                             tttACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTg                             <  2:141204/93‑1 (MQ=255)
                                                             tttACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTg                             >  1:1323094/1‑93 (MQ=255)
                                                              ttccGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTgg                            <  2:2149835/90‑1 (MQ=255)
                                                               tACGCCAGCGTCCCCCCGCCTGCCTACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTggg                           <  1:8953760/93‑1 (MQ=255)
                                                               tACGCCAGCGCCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTggg                           <  2:1066914/93‑1 (MQ=255)
                                                                 cGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGAc                         <  1:7987918/93‑1 (MQ=255)
                                                                   ccAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGAcag                       >  2:8876248/1‑93 (MQ=255)
                                                                    cAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGAcagc                      <  1:7275358/93‑1 (MQ=255)
                                                                     aGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGAcagca                     >  1:5912774/1‑93 (MQ=255)
                                                                      gCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGAcagcag                    >  1:2452391/1‑93 (MQ=255)
                                                                      gCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGAcagcag                    >  2:8528653/1‑93 (MQ=255)
                                                                       cGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACagcagc                   <  2:4736519/93‑1 (MQ=255)
                                                                       cGTCCCCCCGCCTGCAGACAACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACagcagc                   <  2:6234541/93‑1 (MQ=255)
                                                                        gTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAgcc                  >  1:6737168/1‑93 (MQ=255)
                                                                         tCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAgccg                 >  2:7515252/1‑93 (MQ=255)
                                                                         tCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAgccg                 >  2:792983/1‑93 (MQ=255)
                                                                         tCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAgccg                 >  2:6654429/1‑93 (MQ=255)
                                                                          ccccccGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAgccgc                >  2:5858657/1‑93 (MQ=255)
                                                                             cccGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCaa             >  1:4157430/1‑92 (MQ=255)
                                                                              ccGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGa            <  2:686622/93‑1 (MQ=255)
                                                                               cGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGAc           >  2:8700272/1‑93 (MQ=255)
                                                                               cGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGAc           >  1:101163/1‑93 (MQ=255)
                                                                                gCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGGATTTCGCTCGGTTGGGACAGCAGCCACCAGACg          >  2:9113082/1‑93 (MQ=255)
                                                                                  cTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCt        >  2:6955988/1‑93 (MQ=255)
                                                                                   tGCATACTACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTg       <  1:6390031/93‑1 (MQ=255)
                                                                                   tGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTg       >  1:1974994/1‑93 (MQ=255)
                                                                                    gCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTgg      <  2:7826349/93‑1 (MQ=255)
                                                                                    gCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTgg      <  1:3366256/93‑1 (MQ=255)
                                                                                     cATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGa     <  1:2252006/93‑1 (MQ=255)
                                                                                     cATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGa     <  1:8366109/93‑1 (MQ=255)
                                                                                     cATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCAGCGAGACGCTGGa     >  2:5071895/1‑93 (MQ=255)
                                                                                        aCGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATgc  <  2:1961405/93‑1 (MQ=255)
                                                                                           |                                                                                         
TGCCTGGCAAATGTTGAGCGCG‑CCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGGCCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATGC  >  NC_000913/2208415‑2208594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGGCCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATGCGCAG  >  NC_000913/2208412‑2208598
                                                                                              |                                                                                             
TGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGGGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGA                                                                                               >  SRR4302540.297435/1‑94 (MQ=60)
 GATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGAC                                                                                              <  SRR4302540.2045807/94‑1 (MQ=60)
 GATCCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGAC                                                                                              <  SRR4302540.2380793/94‑1 (MQ=60)
  ATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACC                                                                                             >  SRR4302540.3931060/1‑94 (MQ=60)
   TGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCACCCGCCTGCATACGACCT                                                                                            <  SRR4302540.9526528/94‑1 (MQ=60)
    GCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTC                                                                                           >  SRR4302540.1761794/1‑94 (MQ=60)
     CCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCT                                                                                          <  SRR4302540.5463450/94‑1 (MQ=60)
      CTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTT                                                                                         >  SRR4302540.2818564/1‑94 (MQ=60)
      CTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTT                                                                                         >  SRR4302540.1991778/1‑94 (MQ=60)
      CTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTATGCCTTTGGGGGAATATTTACGCCAGCCT‑CCCCCCGCCAGCACACGCCCTCTT                                                                                         >  SRR4302540.2818565/1‑94 (MQ=60)
       TGGCAAATGTTGAGCGCCCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTA                                                                                        <  SRR4302540.8157972/94‑1 (MQ=60)
        GGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAA                                                                                       <  SRR4302540.673781/94‑1 (MQ=60)
         GCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAAT                                                                                      >  SRR4302540.6094776/1‑94 (MQ=60)
         GCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAAT                                                                                      >  SRR4302540.6094775/1‑94 (MQ=60)
          CAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATC                                                                                     >  SRR4302540.3702176/1‑94 (MQ=60)
            AATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTCCGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAG                                                                                   <  SRR4302540.3902959/94‑1 (MQ=60)
            AATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCCGCCTGCATACGACCTCTTAATCA                                                                                    <  SRR4302540.1104762/94‑1 (MQ=60)
              TGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGT                                                                                 >  SRR4302540.5596441/1‑94 (MQ=60)
              TGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGT                                                                                 <  SRR4302540.7133000/94‑1 (MQ=60)
              TGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGC                                                                                 >  SRR4302540.7405833/1‑94 (MQ=60)
               GTTGAGCGCGCGGTTACGCCAGCCGGGCATTGTGGCTTGGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTC                                                                                <  SRR4302540.4654299/94‑1 (MQ=60)
               GTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTC                                                                                <  SRR4302540.9297920/94‑1 (MQ=60)
                TTGAGCGCGCCTTTCCTCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTAG                                                                               <  SRR4302540.6044482/94‑1 (MQ=60)
                TTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCCGCGT‑CCCCCCGCCTGCATACGCCCTCTTAATGAGGTCG                                                                               >  SRR4302540.1623088/1‑94 (MQ=60)
                TTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCG                                                                               <  SRR4302540.4371394/94‑1 (MQ=60)
                 TGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGA                                                                              >  SRR4302540.7658311/1‑94 (MQ=60)
                  TAGCGCCCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGAT                                                                             <  SRR4302540.2063685/94‑1 (MQ=60)
                     CGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAAC                                                                          >  SRR4302540.6920482/1‑94 (MQ=60)
                     CGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAAC                                                                          <  SRR4302540.7286669/94‑1 (MQ=60)
                     CGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAAC                                                                          >  SRR4302540.8792259/1‑94 (MQ=60)
                      GCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACT                                                                         >  SRR4302540.163671/1‑94 (MQ=60)
                      GCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACT                                                                         <  SRR4302540.3115874/94‑1 (MQ=60)
                        GCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTA                                                                       >  SRR4302540.6682263/1‑94 (MQ=60)
                        GCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTA                                                                       <  SRR4302540.3898491/94‑1 (MQ=60)
                        GCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTA                                                                       <  SRR4302540.3846487/94‑1 (MQ=60)
                        GCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTA                                                                       >  SRR4302540.10032531/1‑94 (MQ=60)
                           GTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTT                                                                    >  SRR4302540.3524040/1‑94 (MQ=60)
                           GTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTT                                                                    >  SRR4302540.9629624/1‑94 (MQ=60)
                           GTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTT                                                                    <  SRR4302540.944180/94‑1 (MQ=60)
                           GTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTT                                                                    >  SRR4302540.7823126/1‑94 (MQ=60)
                           GTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCCTAATCAGGTCGATAACTTATTT                                                                    <  SRR4302540.3128312/94‑1 (MQ=60)
                            TTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATCTTTACGCCAGCGT‑CCCCCCGCCTGCATACGCCCTCTTAATCAGGTCGATAACTTATTTA                                                                   >  SRR4302540.7364090/1‑94 (MQ=60)
                            TTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTA                                                                   >  SRR4302540.7364089/1‑94 (MQ=60)
                            TTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTA                                                                   <  SRR4302540.8879124/94‑1 (MQ=60)
                             TACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTAT                                                                  <  SRR4302540.41024/94‑1 (MQ=60)
                             TACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGAGAACTTATTTAT                                                                  >  SRR4302540.6715580/1‑94 (MQ=60)
                              ACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGAATACGACCTCTTAATCAGGTCGATAACTTATTTATT                                                                 <  SRR4302540.1810767/94‑1 (MQ=60)
                               CGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTC                                                                <  SRR4302540.3521790/94‑1 (MQ=60)
                               CGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTC                                                                <  SRR4302540.6597502/94‑1 (MQ=60)
                                GCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCT                                                               <  SRR4302540.4802324/94‑1 (MQ=60)
                                GCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCT                                                               <  SRR4302540.4802322/94‑1 (MQ=60)
                                GCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCT                                                               <  SRR4302540.5841838/94‑1 (MQ=60)
                                CCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCT                                                               <  SRR4302540.2754834/94‑1 (MQ=60)
                                  CAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGC                                                             >  SRR4302540.5027942/1‑94 (MQ=60)
                                   AGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCC                                                            >  SRR4302540.5473083/1‑94 (MQ=60)
                                   AGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCC                                                            >  SRR4302540.6340635/1‑94 (MQ=60)
                                   AGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCC                                                            >  SRR4302540.6340637/1‑94 (MQ=60)
                                   AGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCC                                                            <  SRR4302540.4449278/94‑1 (MQ=60)
                                     CCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGG                                                          >  SRR4302540.7651369/1‑94 (MQ=60)
                                      CGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGT                                                         <  SRR4302540.3801079/94‑1 (MQ=60)
                                      CGGGCATTGGGGCTTTGCGGGAATCTTTCCGCCGGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGT                                                         <  SRR4302540.3801081/94‑1 (MQ=60)
                                       GGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTC                                                        >  SRR4302540.3911021/1‑94 (MQ=60)
                                       GGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTC                                                        <  SRR4302540.9534654/94‑1 (MQ=60)
                                       CGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGGCCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTC                                                        <  SRR4302540.10090913/94‑1 (MQ=60)
                                        GGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCG                                                       <  SRR4302540.9466777/94‑1 (MQ=60)
                                        GGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCG                                                       <  SRR4302540.9138117/94‑1 (MQ=60)
                                        GGCATTGTGGCTGTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCG                                                       <  SRR4302540.9840193/94‑1 (MQ=60)
                                           ATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCTGGTCGCTG                                                    >  SRR4302540.9562924/1‑94 (MQ=60)
                                           ATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTG                                                    >  SRR4302540.4623477/1‑94 (MQ=60)
                                           ATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTG                                                    >  SRR4302540.6575151/1‑94 (MQ=60)
                                           ATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTG                                                    >  SRR4302540.9516455/1‑94 (MQ=60)
                                           ATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTG                                                    >  SRR4302540.5286050/1‑94 (MQ=60)
                                           ATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTG                                                    >  SRR4302540.6575150/1‑94 (MQ=60)
                                           ATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTG                                                    >  SRR4302540.1476354/1‑94 (MQ=60)
                                           ATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTG                                                    >  SRR4302540.6889845/1‑94 (MQ=60)
                                           ATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTG                                                    >  SRR4302540.5769036/1‑94 (MQ=60)
                                           ATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTG                                                    >  SRR4302540.7207386/1‑94 (MQ=60)
                                           ATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTG                                                    >  SRR4302540.305117/1‑94 (MQ=60)
                                            TTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGA                                                   >  SRR4302540.9862236/1‑94 (MQ=60)
                                             TGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAG                                                  >  SRR4302540.4624187/1‑94 (MQ=60)
                                               TGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATACCTTATTTATTCTGCCGGTCGCTGAGTG                                                >  SRR4302540.3162882/1‑94 (MQ=60)
                                                 GCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTA                                              <  SRR4302540.5408813/94‑1 (MQ=60)
                                                  CTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTAT                                             <  SRR4302540.7269981/94‑1 (MQ=60)
                                                  CTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTAT                                             >  SRR4302540.1660919/1‑94 (MQ=60)
                                                    TTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTT                                           <  SRR4302540.7923157/94‑1 (MQ=60)
                                                     TGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTC                                          >  SRR4302540.4471446/1‑94 (MQ=60)
                                                     TGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTAGCTGAGTGTATTTC                                          >  SRR4302540.4471447/1‑94 (MQ=60)
                                                       CGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGC                                        >  SRR4302540.110850/1‑94 (MQ=60)
                                                         GGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTC                                      >  SRR4302540.5440975/1‑94 (MQ=60)
                                                          GAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCG                                     <  SRR4302540.2135460/94‑1 (MQ=60)
                                                          GAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCG                                     >  SRR4302540.2200440/1‑94 (MQ=60)
                                                            ATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGT                                   >  SRR4302540.2691103/1‑94 (MQ=60)
                                                              ATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTG                                 >  SRR4302540.435502/1‑94 (MQ=60)
                                                              ATTTACGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTG                                 >  SRR4302540.1336888/1‑94 (MQ=60)
                                                                TTACGCCACCCT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTAATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGG                               <  SRR4302540.3650171/94‑1 (MQ=60)
                                                                 TACGCCAGCGT‑CCCCCCGCCTGCCTACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGA                              <  SRR4302540.9059413/94‑1 (MQ=60)
                                                                   CGCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACA                            <  SRR4302540.8080929/94‑1 (MQ=60)
                                                                    GCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAG                           <  SRR4302540.6898722/94‑1 (MQ=60)
                                                                    GCCAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAG                           >  SRR4302540.1440131/1‑94 (MQ=60)
                                                                      CAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCA                         >  SRR4302540.327337/1‑94 (MQ=60)
                                                                      CAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCA                         <  SRR4302540.7356536/94‑1 (MQ=60)
                                                                      CAGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCA                         >  SRR4302540.5978098/1‑94 (MQ=60)
                                                                       AGCGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAG                        >  SRR4302540.2478792/1‑94 (MQ=60)
                                                                         CGT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCC                      >  SRR4302540.6811583/1‑94 (MQ=60)
                                                                          GT‑CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCG                     >  SRR4302540.8492134/1‑94 (MQ=60)
                                                                          GT‑CCCCCCGCCTGAATACGACCTCTTAATCAGGTAGATAGCTTATTTCTTATGCCGGTCGCTGAGTGTATTTCGCTGGGTTGGGggggtagcag                     >  SRR4302540.8492135/1‑84 (MQ=58)
                                                                              CCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCA                  >  SRR4302540.3851458/1‑94 (MQ=60)
                                                                               CCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAG                 <  SRR4302540.5944870/94‑1 (MQ=60)
                                                                               CCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAG                 <  SRR4302540.118961/94‑1 (MQ=60)
                                                                               CCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAA                 >  SRR4302540.4202476/1‑94 (MQ=60)
                                                                                 CCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGAC               >  SRR4302540.2594214/1‑94 (MQ=60)
                                                                                 CCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGAC               >  SRR4302540.102235/1‑94 (MQ=60)
                                                                                 CCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGAC               >  SRR4302540.5764055/1‑94 (MQ=60)
                                                                                 CCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGAC               <  SRR4302540.147469/94‑1 (MQ=60)
                                                                                  CGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACG              >  SRR4302540.243769/1‑94 (MQ=60)
                                                                                     CTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTG           >  SRR4302540.4999596/1‑94 (MQ=60)
                                                                                     CTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTG           >  SRR4302540.1996424/1‑94 (MQ=60)
                                                                                     CTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTG           >  SRR4302540.6179429/1‑94 (MQ=60)
                                                                                      TGCATACTACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGG          <  SRR4302540.6461162/94‑1 (MQ=60)
                                                                                       GCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGA         <  SRR4302540.3403078/94‑1 (MQ=60)
                                                                                        CATACGCCCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGAT        <  SRR4302540.2276175/94‑1 (MQ=60)
                                                                                        CATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGAT        <  SRR4302540.8463191/94‑1 (MQ=60)
                                                                                        CATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGAT        <  SRR4302540.2276178/94‑1 (MQ=60)
                                                                                        CATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGAT        >  SRR4302540.7923000/1‑94 (MQ=60)
                                                                                        CATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGAT        <  SRR4302540.7661033/94‑1 (MQ=60)
                                                                                        CATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGAT        <  SRR4302540.2464504/94‑1 (MQ=60)
                                                                                         ATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATG       >  SRR4302540.6009804/1‑94 (MQ=60)
                                                                                            CGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATGCGC    >  SRR4302540.8593668/1‑94 (MQ=60)
                                                                                             GACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATGCGCA   >  SRR4302540.2229977/1‑94 (MQ=60)
                                                                                             GACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATGCGCA   >  SRR4302540.10108080/1‑94 (MQ=60)
                                                                                              ACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATGCGCAG  >  SRR4302540.5312619/1‑94 (MQ=60)
                                                                                              |                                                                                             
TGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGT‑CCCCCCGCCTGCATACGGCCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATGCGCAG  >  NC_000913/2208412‑2208598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑