Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,208,505 G→A R462R (CGG→CGA)  b2122 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,208,5050GA99.6% 881.2 / NA 246R462R (CGG→CGA) b2122hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (123/122);  minor base T (0/1);  total (123/123)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 249 total aligned reads displayed.

TGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGGCCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATGC  >  NC_000913/2208415‑2208594
                                                                                          |                                                                                         
tGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGAcc                                                                                         >  2:2041260/1‑93 (MQ=255)
  ccTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACctc                                                                                       <  2:3333671/93‑1 (MQ=255)
    tGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCtt                                                                                     >  1:638446/1‑93 (MQ=255)
     ggCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTa                                                                                    <  1:5604171/93‑1 (MQ=255)
      gCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTaa                                                                                   >  2:5681049/1‑93 (MQ=255)
      gCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTaa                                                                                   >  1:1821681/1‑93 (MQ=255)
       cAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAAt                                                                                  >  1:852763/1‑93 (MQ=255)
        aaaTGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTAGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATc                                                                                 >  1:3375660/1‑93 (MQ=255)
         aaTGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCa                                                                                >  1:1717827/1‑93 (MQ=255)
          aTGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAg                                                                               >  1:3126225/1‑93 (MQ=255)
           tGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAgg                                                                              >  2:4914457/1‑93 (MQ=255)
             ttGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTc                                                                            <  1:5365731/93‑1 (MQ=255)
             ttGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTc                                                                            >  1:1524840/1‑93 (MQ=255)
             ttGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTc                                                                            <  2:5048045/93‑1 (MQ=255)
             ttGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTc                                                                            >  2:5981977/1‑93 (MQ=255)
             ttGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTGGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTc                                                                            <  2:3845629/93‑1 (MQ=255)
              tGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCg                                                                           <  1:4711294/93‑1 (MQ=255)
                aGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGAt                                                                         >  2:5337712/1‑93 (MQ=255)
                 gcgcgcCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATa                                                                        <  1:2210122/93‑1 (MQ=255)
                    cgcCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACt                                                                     <  2:4559568/93‑1 (MQ=255)
                    cgcCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACt                                                                     >  2:4096985/1‑93 (MQ=255)
                     gcCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACtt                                                                    >  1:1442265/1‑93 (MQ=255)
                      ccGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACtta                                                                   >  1:5984079/1‑93 (MQ=255)
                       cGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTCCGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACttat                                                                  <  1:4213085/93‑1 (MQ=255)
                        gTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACttatt                                                                 >  1:576258/1‑93 (MQ=255)
                        gTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACttatt                                                                 >  1:6156603/1‑93 (MQ=255)
                        gTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACttatt                                                                 <  1:2041603/93‑1 (MQ=255)
                         ttACGCGAGCCGGGCATTGTGGCTTTGCGGGAATATTTCCGCCAGCGCCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACttattt                                                                <  2:1014138/93‑1 (MQ=255)
                          tACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACttattta                                                               <  2:5416965/93‑1 (MQ=255)
                             gCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTCATTTATTc                                                            <  2:6165667/93‑1 (MQ=255)
                             cccAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTc                                                            <  1:6121882/92‑1 (MQ=255)
                              ccAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCt                                                           <  1:6120308/93‑1 (MQ=255)
                               cAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTg                                                          <  2:6110011/93‑1 (MQ=255)
                                 gCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGcc                                                        <  2:4460720/93‑1 (MQ=255)
                                  ccGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCg                                                       <  1:3749137/93‑1 (MQ=255)
                                  ccGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCg                                                       <  2:3411338/93‑1 (MQ=255)
                                  ccGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCg                                                       <  1:4578830/93‑1 (MQ=255)
                                  ccGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCg                                                       >  1:2915929/1‑93 (MQ=255)
                                  ccGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCg                                                       <  1:737389/93‑1 (MQ=255)
                                   cGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCgg                                                      >  2:2868622/1‑93 (MQ=255)
                                    gggCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGt                                                     >  1:3993633/1‑93 (MQ=255)
                                    gggCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGt                                                     >  1:2138297/1‑93 (MQ=255)
                                     ggCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTc                                                    >  1:434898/1‑93 (MQ=255)
                                     ggCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTc                                                    >  2:3939468/1‑93 (MQ=255)
                                     ggCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCACTTAATCAGGGCGATAACTTATTTATTCTGCCGGTc                                                    >  2:1179412/1‑93 (MQ=255)
                                       cATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGc                                                  >  2:719694/1‑93 (MQ=255)
                                        aTTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCt                                                 >  2:2466736/1‑93 (MQ=255)
                                         ttGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTg                                                >  2:4552313/1‑93 (MQ=255)
                                          tgtgGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGa                                               <  1:408153/93‑1 (MQ=255)
                                          tggggCTTTGCGAAAATATTTACGCCACCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGCCGATAACTTATTTATTCTGCCGGTCGCTGa                                               <  2:5111659/90‑1 (MQ=255)
                                           gtgGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAg                                              <  1:1136683/93‑1 (MQ=255)
                                           gtgGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAg                                              >  1:5473471/1‑93 (MQ=255)
                                             ggCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAgtg                                            <  1:669775/93‑1 (MQ=255)
                                              gCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAgtgt                                           <  2:5586755/93‑1 (MQ=255)
                                                tttGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTAt                                         >  2:1382084/1‑93 (MQ=255)
                                                tttGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTAt                                         <  2:2377439/93‑1 (MQ=255)
                                                 ttGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTAtt                                        <  1:4918373/93‑1 (MQ=255)
                                                   gCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTc                                      >  2:1638963/1‑93 (MQ=255)
                                                    cGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCg                                     >  2:5238736/1‑93 (MQ=255)
                                                    cGGGAATATTTACGCCAGCGCCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTTTATTTCg                                     <  1:5613944/93‑1 (MQ=255)
                                                     gggAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCgt                                    >  2:4366875/1‑92 (MQ=255)
                                                     gggAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGc                                    >  1:5095047/1‑93 (MQ=255)
                                                      ggAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCt                                   >  1:2841376/1‑93 (MQ=255)
                                                       gAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTc                                  <  1:1630094/93‑1 (MQ=255)
                                                           atTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGtt                              <  1:3211967/93‑1 (MQ=255)
                                                             ttACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTgg                            >  1:3877124/1‑93 (MQ=255)
                                                                cGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGAc                         <  2:1946733/93‑1 (MQ=255)
                                                                 gCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGAca                        >  2:5847872/1‑93 (MQ=255)
                                                                    agagTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACCTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGAcagca                     >  2:5493943/4‑93 (MQ=255)
                                                                    aGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGAcagca                     >  1:804111/1‑93 (MQ=255)
                                                                    aGCGCCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGAcagca                     <  2:284322/93‑1 (MQ=255)
                                                                     gCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGAcagcag                    <  2:1627705/93‑1 (MQ=255)
                                                                      cGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACagcagc                   <  2:1918936/93‑1 (MQ=255)
                                                                      cGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACagcagc                   <  1:4271226/93‑1 (MQ=255)
                                                                        tCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACagcagcag                 >  1:1024533/1‑91 (MQ=255)
                                                                         ccccccGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAgccgc                <  1:5873977/93‑1 (MQ=255)
                                                                          cccccGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAgccgcc               >  1:1567010/1‑93 (MQ=255)
                                                                          cccccGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAgccgcc               >  1:2498441/1‑93 (MQ=255)
                                                                           ccccGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCa              >  1:1046688/1‑93 (MQ=255)
                                                                           ccccGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCa              <  1:1290178/93‑1 (MQ=255)
                                                                            cccGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAg             >  1:5946254/1‑93 (MQ=255)
                                                                             ccGCCTTCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGa            <  2:1506827/93‑1 (MQ=255)
                                                                             ccGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGa            >  2:3663882/1‑93 (MQ=255)
                                                                             ccGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGa            <  1:4019826/93‑1 (MQ=255)
                                                                              cGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGAc           >  1:3573751/1‑93 (MQ=255)
                                                                                ccTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGc         >  2:2087859/1‑93 (MQ=255)
                                                                                 cTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCt        <  2:2727796/93‑1 (MQ=255)
                                                                                 cTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCt        >  1:34124/1‑93 (MQ=255)
                                                                                  tGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTg       >  1:4865903/1‑93 (MQ=255)
                                                                                  tGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTg       >  2:5786732/1‑93 (MQ=255)
                                                                                   gCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTgg      <  2:4749036/93‑1 (MQ=255)
                                                                                    cATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGa     <  2:116015/93‑1 (MQ=255)
                                                                                    cATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGa     <  2:4212676/93‑1 (MQ=255)
                                                                                    cATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGa     <  1:3662278/93‑1 (MQ=255)
                                                                                     aTACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGAt    <  1:2687048/93‑1 (MQ=255)
                                                                                     aTACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGAt    >  1:1424756/1‑93 (MQ=255)
                                                                                     aTACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGAt    >  1:5253873/1‑93 (MQ=255)
                                                                                      tACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATg   >  1:499047/1‑93 (MQ=255)
                                                                                      tACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATg   <  1:5496395/93‑1 (MQ=255)
                                                                                      tACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATg   <  1:125670/93‑1 (MQ=255)
                                                                                          |                                                                                         
TGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGGCCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATGC  >  NC_000913/2208415‑2208594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGGCCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATGCGCAG  >  NC_000913/2208412‑2208598
                                                                                             |                                                                                             
TGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGA                                                                                               >  SRR4302533.560998/1‑94 (MQ=60)
TGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGA                                                                                               <  SRR4302533.1372652/94‑1 (MQ=60)
TGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCACGCCTGCATACGA                                                                                               >  SRR4302533.2831533/1‑94 (MQ=60)
 GATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGAC                                                                                              <  SRR4302533.1076301/94‑1 (MQ=60)
  ATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACC                                                                                             <  SRR4302533.1789162/94‑1 (MQ=60)
      CTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTT                                                                                         >  SRR4302533.642201/1‑94 (MQ=60)
      CTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTT                                                                                         <  SRR4302533.145565/94‑1 (MQ=60)
      CTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTT                                                                                         >  SRR4302533.2867315/1‑94 (MQ=60)
       TGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGTATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTA                                                                                        <  SRR4302533.6333982/94‑1 (MQ=60)
        GGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAA                                                                                       >  SRR4302533.1832806/1‑94 (MQ=60)
        GGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAA                                                                                       <  SRR4302533.5638029/94‑1 (MQ=60)
         GCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAAT                                                                                      <  SRR4302533.4073264/94‑1 (MQ=60)
         GCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAAT                                                                                      >  SRR4302533.857756/1‑94 (MQ=60)
         GCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAAT                                                                                      >  SRR4302533.375000/1‑94 (MQ=60)
         GCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTGGCGGGAATATTTACGCCAGCGCCCCCCCGCCTGCATACGACCTCTTAAT                                                                                      <  SRR4302533.2405568/94‑1 (MQ=60)
          CAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATC                                                                                     >  SRR4302533.668827/1‑94 (MQ=60)
          CAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTAGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATC                                                                                     >  SRR4302533.3396506/1‑94 (MQ=60)
           AAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCA                                                                                    >  SRR4302533.1728241/1‑94 (MQ=60)
            AATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAG                                                                                   >  SRR4302533.3145441/1‑94 (MQ=60)
             ATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGG                                                                                  <  SRR4302533.3201432/94‑1 (MQ=60)
               GTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTC                                                                                >  SRR4302533.1534020/1‑94 (MQ=60)
               GTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTC                                                                                <  SRR4302533.5855509/94‑1 (MQ=60)
                TTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCG                                                                               <  SRR4302533.5397963/94‑1 (MQ=60)
                TTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCG                                                                               <  SRR4302533.5509787/94‑1 (MQ=60)
                 TGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGA                                                                              <  SRR4302533.4739848/94‑1 (MQ=60)
                  GAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGAT                                                                             <  SRR4302533.530584/94‑1 (MQ=60)
                   AGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATA                                                                            <  SRR4302533.1574988/94‑1 (MQ=60)
                    GCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAA                                                                           <  SRR4302533.2223753/94‑1 (MQ=60)
                    GCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAA                                                                           >  SRR4302533.4967599/1‑94 (MQ=60)
                     CGCGCCGTTACGCCAGCCGGGCATTGGGTCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAAC                                                                          <  SRR4302533.3129519/94‑1 (MQ=60)
                      GCGCCTTTACGCCAGCCGTGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGTCCTCTTAATCAGGTCGATAACT                                                                         <  SRR4302533.5723796/94‑1 (MQ=60)
                       CGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTT                                                                        >  SRR4302533.3837609/1‑94 (MQ=60)
                       CGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTT                                                                        >  SRR4302533.1450966/1‑94 (MQ=60)
                        GCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTA                                                                       >  SRR4302533.5578571/1‑94 (MQ=60)
                        GCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTA                                                                       >  SRR4302533.614185/1‑94 (MQ=60)
                        GCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTA                                                                       >  SRR4302533.5137683/1‑94 (MQ=60)
                        GCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTA                                                                       >  SRR4302533.393378/1‑94 (MQ=60)
                        GCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTA                                                                       >  SRR4302533.6020319/1‑94 (MQ=60)
                          CGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTCCGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATT                                                                     <  SRR4302533.4238571/94‑1 (MQ=60)
                          CGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATT                                                                     >  SRR4302533.6193641/1‑94 (MQ=60)
                          CGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATT                                                                     >  SRR4302533.3665251/1‑94 (MQ=60)
                          CGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATT                                                                     >  SRR4302533.4912169/1‑94 (MQ=60)
                          CGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATT                                                                     >  SRR4302533.579658/1‑94 (MQ=60)
                           TTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTT                                                                    <  SRR4302533.5283579/94‑1 (MQ=60)
                           GTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTT                                                                    >  SRR4302533.3720684/1‑94 (MQ=60)
                           GTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTT                                                                    <  SRR4302533.4797618/94‑1 (MQ=60)
                           GTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTT                                                                    <  SRR4302533.1444698/94‑1 (MQ=60)
                           GTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTT                                                                    <  SRR4302533.2054184/94‑1 (MQ=60)
                           CTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTT                                                                    <  SRR4302533.557481/94‑1 (MQ=60)
                            TTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTCCGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTA                                                                   <  SRR4302533.1603462/94‑1 (MQ=60)
                            TTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTA                                                                   <  SRR4302533.1217794/94‑1 (MQ=60)
                            TTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTA                                                                   <  SRR4302533.4076257/94‑1 (MQ=60)
                             TACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGCCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTAT                                                                  <  SRR4302533.6184015/94‑1 (MQ=60)
                               CGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTC                                                                <  SRR4302533.1348216/94‑1 (MQ=60)
                                GCGAGCGGGGCGTTGTGGCTGTGCGGTAATATTTACGCCAGCTACCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCT                                                               <  SRR4302533.834586/94‑1 (MQ=60)
                                CCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCT                                                               <  SRR4302533.6158795/94‑1 (MQ=60)
                                CCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGCCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCT                                                               <  SRR4302533.5936334/94‑1 (MQ=60)
                                 CCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTG                                                              <  SRR4302533.6157214/94‑1 (MQ=60)
                                  CAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGC                                                             <  SRR4302533.4114994/94‑1 (MQ=60)
                                   AGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCATCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCC                                                            >  SRR4302533.6209655/1‑94 (MQ=60)
                                    GCTGGACATTGTGCCTTTGCTGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCG                                                           <  SRR4302533.4210821/94‑1 (MQ=60)
                                    GCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCG                                                           >  SRR4302533.2933810/1‑94 (MQ=60)
                                     CCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGG                                                          <  SRR4302533.741731/94‑1 (MQ=60)
                                     CCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGG                                                          <  SRR4302533.3772121/94‑1 (MQ=60)
                                     CCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGG                                                          >  SRR4302533.2297979/1‑94 (MQ=60)
                                     CCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGG                                                          <  SRR4302533.4606581/94‑1 (MQ=60)
                                      CGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGT                                                         >  SRR4302533.2151481/1‑94 (MQ=60)
                                      CGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGT                                                         >  SRR4302533.4017952/1‑94 (MQ=60)
                                      CGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGT                                                         >  SRR4302533.1735705/1‑94 (MQ=60)
                                       GGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTC                                                        >  SRR4302533.437489/1‑94 (MQ=60)
                                       GGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTC                                                        <  SRR4302533.4646161/94‑1 (MQ=60)
                                        GGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCG                                                       <  SRR4302533.5642132/94‑1 (MQ=60)
                                         GCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGC                                                      >  SRR4302533.3286534/1‑94 (MQ=60)
                                         GCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGC                                                      <  SRR4302533.2763401/94‑1 (MQ=60)
                                         GCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGC                                                      >  SRR4302533.1136275/1‑94 (MQ=60)
                                          CATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCT                                                     >  SRR4302533.604222/1‑94 (MQ=60)
                                          CATGGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCT                                                     <  SRR4302533.49623/94‑1 (MQ=60)
                                             TGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAG                                                  >  SRR4302533.5506463/1‑94 (MQ=60)
                                             TGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAG                                                  <  SRR4302533.410606/94‑1 (MQ=60)
                                              GTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGT                                                 <  SRR4302533.1143442/94‑1 (MQ=60)
                                                GGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGT                                               <  SRR4302533.3474438/94‑1 (MQ=60)
                                                GGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGT                                               <  SRR4302533.673734/94‑1 (MQ=60)
                                                 GCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTA                                              <  SRR4302533.4452655/94‑1 (MQ=60)
                                                 GCTTTGCGAGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTA                                              <  SRR4302533.719535/94‑1 (MQ=60)
                                                  CTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTAT                                             >  SRR4302533.1148509/1‑94 (MQ=60)
                                                  CTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTAT                                             >  SRR4302533.4373434/1‑94 (MQ=60)
                                                   TTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTNNTCTGCCGGTCGCTGGNTGTNTT                                            >  SRR4302533.4837659/1‑94 (MQ=60)
                                                    TTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTT                                           <  SRR4302533.4948168/94‑1 (MQ=60)
                                                    TTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTT                                           >  SRR4302533.2197569/1‑94 (MQ=60)
                                                     tgggggaagttttacgcgccgGTCCCCCCGCCTGCATACGACCGCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTC                                          <  SRR4302533.1525731/73‑1 (MQ=60)
                                                     TGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTC                                          <  SRR4302533.1046136/94‑1 (MQ=60)
                                                     TGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTC                                          <  SRR4302533.5051502/94‑1 (MQ=60)
                                                       CGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGC                                        >  SRR4302533.5125827/1‑94 (MQ=60)
                                                       CGGGAATATTTACGCCAGCGCCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTTTATTTCGC                                        <  SRR4302533.5647876/94‑1 (MQ=60)
                                                        GGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCT                                       >  SRR4302533.2858861/1‑94 (MQ=60)
                                                          GAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCG                                     <  SRR4302533.4267623/94‑1 (MQ=60)
                                                          GAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCG                                     <  SRR4302533.1639969/94‑1 (MQ=60)
                                                          GAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCG                                     <  SRR4302533.833140/94‑1 (MQ=60)
                                                           AATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGG                                    >  SRR4302533.4256518/1‑94 (MQ=60)
                                                            ATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGT                                   >  SRR4302533.4037152/1‑94 (MQ=60)
                                                            ATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGT                                   <  SRR4302533.2234505/94‑1 (MQ=60)
                                                            ATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGT                                   >  SRR4302533.2059527/1‑94 (MQ=60)
                                                             TATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTT                                  >  SRR4302533.2843066/1‑94 (MQ=60)
                                                             TATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTT                                  >  SRR4302533.1730160/1‑94 (MQ=60)
                                                              ATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTG                                 <  SRR4302533.3231751/94‑1 (MQ=60)
                                                               TTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGG                                <  SRR4302533.3974867/94‑1 (MQ=60)
                                                               TTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGG                                >  SRR4302533.3900786/1‑94 (MQ=60)
                                                                 TACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGA                              >  SRR4302533.3353229/1‑94 (MQ=60)
                                                                  ACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGAC                             <  SRR4302533.1924374/94‑1 (MQ=60)
                                                                  ACGCAAGCGGCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGAC                             <  SRR4302533.1683154/94‑1 (MQ=60)
                                                                    GCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAG                           >  SRR4302533.2101197/1‑94 (MQ=60)
                                                                     caacccTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGC                          <  SRR4302533.4863254/88‑1 (MQ=60)
                                                                      CAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCA                         >  SRR4302533.808835/1‑94 (MQ=60)
                                                                       AGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAG                        >  SRR4302533.5451966/1‑94 (MQ=60)
                                                                       AGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAG                        >  SRR4302533.3428770/1‑94 (MQ=60)
                                                                       AGCGCCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAG                        <  SRR4302533.5932307/94‑1 (MQ=60)
                                                                        GCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGC                       <  SRR4302533.1592794/94‑1 (MQ=60)
                                                                         CGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCC                      >  SRR4302533.5449122/1‑94 (MQ=60)
                                                                         CGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCC                      <  SRR4302533.4296933/94‑1 (MQ=60)
                                                                          GTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCG                     >  SRR4302533.6158400/1‑94 (MQ=60)
                                                                          GTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCAG                     >  SRR4302533.1030556/1‑94 (MQ=60)
                                                                            CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCC                   >  SRR4302533.2513855/1‑94 (MQ=60)
                                                                            CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCC                   >  SRR4302533.1576445/1‑94 (MQ=60)
                                                                            CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCC                   <  SRR4302533.5909481/94‑1 (MQ=60)
                                                                             CCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCA                  >  SRR4302533.1395367/1‑94 (MQ=60)
                                                                             CCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCA                  >  SRR4302533.1052838/1‑94 (MQ=60)
                                                                             CCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCA                  <  SRR4302533.3445685/94‑1 (MQ=60)
                                                                              CCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAG                 <  SRR4302533.1297946/94‑1 (MQ=60)
                                                                              CCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAG                 >  SRR4302533.5982215/1‑94 (MQ=60)
                                                                              CCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAG                 >  SRR4302533.904277/1‑94 (MQ=60)
                                                                               CCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGA                <  SRR4302533.684365/94‑1 (MQ=60)
                                                                               CCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGA                >  SRR4302533.1514188/1‑94 (MQ=60)
                                                                                CCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGAC               <  SRR4302533.4044304/94‑1 (MQ=60)
                                                                                CCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGAC               >  SRR4302533.3595772/1‑94 (MQ=60)
                                                                                   CCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCT            >  SRR4302533.2613203/1‑94 (MQ=60)
                                                                                   CCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCT            >  SRR4302533.34356/1‑94 (MQ=60)
                                                                                    CTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTG           >  SRR4302533.4895390/1‑94 (MQ=60)
                                                                                    CTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTG           <  SRR4302533.6018208/94‑1 (MQ=60)
                                                                                    CTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTG           <  SRR4302533.5763317/94‑1 (MQ=60)
                                                                                     TGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGG          >  SRR4302533.794744/1‑94 (MQ=60)
                                                                                      GCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGA         >  SRR4302533.4580264/1‑94 (MQ=60)
                                                                                      GCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGA         <  SRR4302533.1271641/94‑1 (MQ=60)
                                                                                       CATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGAT        >  SRR4302533.1433374/1‑94 (MQ=60)
                                                                                       CATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGAT        >  SRR4302533.5285438/1‑94 (MQ=60)
                                                                                       CATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGAT        <  SRR4302533.3684792/94‑1 (MQ=60)
                                                                                        ATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATG       >  SRR4302533.502017/1‑94 (MQ=60)
                                                                                        ATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATG       <  SRR4302533.2703521/94‑1 (MQ=60)
                                                                                         TACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATGC      <  SRR4302533.5529547/94‑1 (MQ=60)
                                                                                         TACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATGC      <  SRR4302533.126455/94‑1 (MQ=60)
                                                                                          ACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATGCG     <  SRR4302533.3008338/94‑1 (MQ=60)
                                                                                          ACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATGCG     >  SRR4302533.4817056/1‑94 (MQ=60)
                                                                                           CGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATGCGC    >  SRR4302533.370707/1‑94 (MQ=60)
                                                                                            GACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATGCGCA   >  SRR4302533.2870109/1‑94 (MQ=60)
                                                                                            GACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATGCGCA   <  SRR4302533.4533564/94‑1 (MQ=60)
                                                                                             ACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATGCGCAG  >  SRR4302533.2172513/1‑94 (MQ=60)
                                                                                             ACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATGCGCAG  <  SRR4302533.514365/94‑1 (MQ=60)
                                                                                             ACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATGCGCAG  >  SRR4302533.2055677/1‑94 (MQ=60)
                                                                                             |                                                                                             
TGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGGCCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATGCGCAG  >  NC_000913/2208412‑2208598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑