Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,232,157 T→C S35S (AGT→AGC)  b2146 → putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,232,1570TC95.0% 884.0 / 23.1 303S35S (AGT→AGC) b2146putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (7/8);  new base C (149/139);  total (156/147)
Fisher's exact test for biased strand distribution p-value = 7.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.99e-01

Only 100 of 314 total aligned reads displayed.

TTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGA  >  NC_000913/2232067‑2232246
                                                                                          |                                                                                         
ttATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCa                                                                                         <  2:4058193/93‑1 (MQ=255)
  aTCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAgg                                                                                       >  1:1548895/1‑93 (MQ=255)
  aTCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAgg                                                                                       <  2:7457410/93‑1 (MQ=255)
  aTCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAgg                                                                                       >  1:5381468/1‑93 (MQ=255)
  aTCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTAACGACGATCCCTGTACCCAgg                                                                                       >  2:3215096/1‑93 (MQ=255)
    cTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCt                                                                                     <  2:1983438/93‑1 (MQ=255)
    cTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCt                                                                                     <  2:5458361/93‑1 (MQ=255)
      ggATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTg                                                                                   >  2:9088047/1‑93 (MQ=255)
        aTGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGcc                                                                                 <  2:8343949/93‑1 (MQ=255)
         tGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGccc                                                                                <  2:2432136/93‑1 (MQ=255)
         tGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGccc                                                                                <  1:2546050/93‑1 (MQ=255)
         tGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTAACGAGGCTCCCTGTGGCAAGGCTTcccg                                                                                >  2:3399432/1‑92 (MQ=255)
            actcactcCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGc                                                                             <  2:3572094/93‑1 (MQ=255)
               cactcCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGcaca                                                                          <  1:926603/93‑1 (MQ=255)
               cactcCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGcaca                                                                          <  2:1261051/93‑1 (MQ=255)
               cactcCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGcaca                                                                          <  1:7156753/93‑1 (MQ=255)
                 ctcCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGa                                                                        <  1:7909938/93‑1 (MQ=255)
                 ctcCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGa                                                                        >  1:986623/1‑93 (MQ=255)
                 ctcCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGa                                                                        <  1:5540805/93‑1 (MQ=255)
                  tcCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGAc                                                                       >  1:4295972/1‑93 (MQ=255)
                  tcCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGAc                                                                       <  1:10013599/93‑1 (MQ=255)
                  tcCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGAc                                                                       <  1:494452/93‑1 (MQ=255)
                     ggCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGa                                                                    <  2:6585127/93‑1 (MQ=255)
                     ggCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGa                                                                    >  2:110301/1‑93 (MQ=255)
                        ttttACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATcc                                                                 <  2:8293112/93‑1 (MQ=255)
                        ttttACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATcc                                                                 >  2:7923492/1‑93 (MQ=255)
                         tttACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCg                                                                >  2:986906/1‑93 (MQ=255)
                          ttACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCgg                                                               >  2:8753690/1‑93 (MQ=255)
                          ttACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGCCACAGACCGATGCgg                                                               >  1:3221514/1‑93 (MQ=255)
                           tACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCggg                                                              <  2:4305044/93‑1 (MQ=255)
                           tACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCggg                                                              >  2:4478319/1‑93 (MQ=255)
                               tCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGaaa                                                          >  1:5663860/1‑93 (MQ=255)
                               tCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGaaa                                                          >  2:4020148/1‑93 (MQ=255)
                                cTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGAGAAAt                                                         >  2:3262006/1‑93 (MQ=255)
                                 tttACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAAtt                                                        >  2:9237187/1‑93 (MQ=255)
                                 tttACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAAtt                                                        <  1:6441947/93‑1 (MQ=255)
                                     cTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTAtt                                                    <  2:2664893/93‑1 (MQ=255)
                                     cTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTAtt                                                    <  2:6706099/93‑1 (MQ=255)
                                      tGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTc                                                   >  2:4605203/1‑93 (MQ=255)
                                      tGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTc                                                   <  1:3410299/93‑1 (MQ=255)
                                       ggCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCg                                                  <  2:1387676/93‑1 (MQ=255)
                                        gCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGc                                                 <  2:4113430/93‑1 (MQ=255)
                                          gATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTc                                               <  1:5693882/93‑1 (MQ=255)
                                          gATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATACGGGGAAATTTATTCGCTc                                               >  2:2956531/1‑93 (MQ=255)
                                           aTTAAATAAACCTCTCGCTGTTTATTATGTCACGGCCCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCa                                              <  1:6961973/93‑1 (MQ=255)
                                           aTTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGCAATTTATTCGCTCa                                              >  1:2309149/1‑93 (MQ=255)
                                           aTTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCa                                              >  2:6464903/1‑93 (MQ=255)
                                            ttAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGCTCCGGGGAACTTTATTCGCTCaa                                             >  2:3950259/1‑93 (MQ=255)
                                             tAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAAt                                            <  2:5233395/93‑1 (MQ=255)
                                             tAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAAt                                            >  2:5574879/1‑93 (MQ=255)
                                              aaaGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATc                                           >  1:2885491/1‑93 (MQ=255)
                                               aagaagCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCt                                          >  1:1077246/1‑93 (MQ=255)
                                                agaagCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGACATTTATTCGCTCAATCTa                                         >  2:1216435/1‑93 (MQ=255)
                                                agaagCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTa                                         >  2:5001083/1‑93 (MQ=255)
                                                 gaagCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTAc                                        >  1:8345647/1‑93 (MQ=255)
                                                   agCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCAACtt                                      <  2:8981960/93‑1 (MQ=255)
                                                    gCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACttt                                     >  1:7788469/1‑93 (MQ=255)
                                                     ccTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTc                                    <  2:5420369/93‑1 (MQ=255)
                                                      ctctCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCg                                   >  1:1440651/1‑93 (MQ=255)
                                                         tcGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTaa                                >  1:8120410/1‑93 (MQ=255)
                                                         tcGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTaa                                <  2:9377129/93‑1 (MQ=255)
                                                          cGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAAt                               <  2:4225004/93‑1 (MQ=255)
                                                             tGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAAtttt                            >  2:1525283/1‑93 (MQ=255)
                                                             tGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAAtttt                            >  1:4658161/1‑93 (MQ=255)
                                                             tGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAAtttt                            >  2:6213191/1‑93 (MQ=255)
                                                              gTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTATTTTCGTGATTTTa                           >  2:8493500/1‑93 (MQ=255)
                                                                ttattaTGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTaaa                         <  1:6192524/93‑1 (MQ=255)
                                                                ttattaTGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTaaa                         <  1:1787782/93‑1 (MQ=255)
                                                                ttattaTGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTaaa                         >  1:9375974/1‑93 (MQ=255)
                                                                  attatGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAgg                       <  1:8571454/93‑1 (MQ=255)
                                                                  attatGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAgg                       >  1:3550893/1‑93 (MQ=255)
                                                                  attatGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAgg                       <  2:9736450/93‑1 (MQ=255)
                                                                  attatGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAgg                       >  2:5624648/1‑93 (MQ=255)
                                                                   ttatGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGgc                      <  1:4465452/93‑1 (MQ=255)
                                                                   ttatGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGgc                      <  1:8001318/93‑1 (MQ=255)
                                                                    tatGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGCAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGgcg                     >  2:5949643/1‑93 (MQ=255)
                                                                    tatGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGgcg                     <  2:2046846/93‑1 (MQ=255)
                                                                     atGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGgcgc                    <  1:3644880/93‑1 (MQ=255)
                                                                     atGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGgcgc                    <  1:1682322/93‑1 (MQ=255)
                                                                       gTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTg                  >  1:6556800/1‑93 (MQ=255)
                                                                        tCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGc                 <  1:3924385/93‑1 (MQ=255)
                                                                        tCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGc                 >  1:6710212/1‑93 (MQ=255)
                                                                        tCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGc                 <  2:2215520/93‑1 (MQ=255)
                                                                        tCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTAGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGc                 <  1:5834475/93‑1 (MQ=255)
                                                                         cACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGcc                <  2:71199/93‑1 (MQ=255)
                                                                             acgCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCgaga            <  2:5857578/93‑1 (MQ=255)
                                                                              cgCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGAc           <  1:2100305/93‑1 (MQ=255)
                                                                                 tCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAAt        <  1:5253626/93‑1 (MQ=255)
                                                                                 tCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAAt        <  2:7000998/93‑1 (MQ=255)
                                                                                  cccTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAAtt       <  2:2574984/93‑1 (MQ=255)
                                                                                  cccTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAAtt       >  2:3020325/1‑93 (MQ=255)
                                                                                   ccTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTc      <  1:4136680/93‑1 (MQ=255)
                                                                                   ccTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGCGACAATTc      >  2:2865314/1‑93 (MQ=255)
                                                                                   ccTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTc      >  1:7625331/1‑93 (MQ=255)
                                                                                   ccTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTc      >  1:9563150/1‑93 (MQ=255)
                                                                                   ccTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTc      >  1:4816366/1‑93 (MQ=255)
                                                                                   ccTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTc      <  1:8856601/93‑1 (MQ=255)
                                                                                    cTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTcg     <  1:6970836/93‑1 (MQ=255)
                                                                                     tGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTcgc    >  1:1139496/1‑93 (MQ=255)
                                                                                       tAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGa  >  2:6098266/1‑93 (MQ=255)
                                                                                          |                                                                                         
TTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGA  >  NC_000913/2232067‑2232246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAAT  >  NC_000913/2232065‑2232250
                                                                                            |                                                                                             
AATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCC                                                                                              >  SRR4302540.2617646/1‑94 (MQ=60)
AATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCC                                                                                              <  SRR4302540.3262361/94‑1 (MQ=60)
   TATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGG                                                                                           >  SRR4302540.6550034/1‑94 (MQ=60)
   TATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGG                                                                                           >  SRR4302540.5440656/1‑94 (MQ=60)
   TATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGG                                                                                           >  SRR4302540.1565975/1‑94 (MQ=60)
   TATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGG                                                                                           >  SRR4302540.6772395/1‑94 (MQ=60)
      CTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTT                                                                                        <  SRR4302540.4225551/94‑1 (MQ=60)
      CTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTT                                                                                        <  SRR4302540.168481/94‑1 (MQ=60)
       TGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTC                                                                                       >  SRR4302540.3113032/1‑94 (MQ=60)
         GATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCC                                                                                     <  SRR4302540.8100786/94‑1 (MQ=60)
         GATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCC                                                                                     <  SRR4302540.8624318/94‑1 (MQ=60)
          ATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCC                                                                                    >  SRR4302540.1720387/1‑94 (MQ=60)
           TGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCG                                                                                   <  SRR4302540.2573493/94‑1 (MQ=60)
             AACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGC                                                                                 >  SRR4302540.2634576/1‑94 (MQ=60)
             AACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGCCACGCCGCTCCCTGTAGCCAGGCTGGCCCGGC                                                                                 >  SRR4302540.2634581/1‑94 (MQ=60)
               CTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCAC                                                                               <  SRR4302540.7185621/94‑1 (MQ=60)
               CTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCAC                                                                               >  SRR4302540.8409177/1‑94 (MQ=60)
               CTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCAC                                                                               >  SRR4302540.9553517/1‑94 (MQ=60)
                TCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACA                                                                              >  SRR4302540.5982114/1‑94 (MQ=60)
                 CACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAG                                                                             <  SRR4302540.936360/94‑1 (MQ=60)
                 CACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAG                                                                             <  SRR4302540.7236521/94‑1 (MQ=60)
                 CACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAG                                                                             <  SRR4302540.8203027/94‑1 (MQ=60)
                  CCTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGA                                                                            <  SRR4302540.6951087/94‑1 (MQ=60)
                  ACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTgtccccgcacagc                                                                            >  SRR4302540.996384/1‑81 (MQ=60)
                  ACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGA                                                                            >  SRR4302540.996705/1‑94 (MQ=60)
                  ACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGA                                                                            >  SRR4302540.2533807/1‑94 (MQ=60)
                   CTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGAC                                                                           <  SRR4302540.8002172/94‑1 (MQ=60)
                   CTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGAC                                                                           <  SRR4302540.4511646/94‑1 (MQ=60)
                   CTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGAC                                                                           >  SRR4302540.4342718/1‑94 (MQ=60)
                   CTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGAC                                                                           <  SRR4302540.5601833/94‑1 (MQ=60)
                    TCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACC                                                                          <  SRR4302540.499753/94‑1 (MQ=60)
                    TCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACC                                                                          <  SRR4302540.10130181/94‑1 (MQ=60)
                       GGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGAT                                                                       <  SRR4302540.1554270/94‑1 (MQ=60)
                       GGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGAT                                                                       >  SRR4302540.3464363/1‑94 (MQ=60)
                       CGCTTTTACGTCTTTACTGGCGATTAGAGAAGCCTCTCGCTGTTTATTATGTCAAGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGAT                                                                       <  SRR4302540.2255960/94‑1 (MQ=60)
                        GCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATC                                                                      <  SRR4302540.7868476/94‑1 (MQ=60)
                         CTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCC                                                                     <  SRR4302540.660823/94‑1 (MQ=60)
                         CTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCC                                                                     <  SRR4302540.3473079/94‑1 (MQ=60)
                          TTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCG                                                                    >  SRR4302540.7091259/1‑94 (MQ=60)
                          TTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCG                                                                    <  SRR4302540.5940357/94‑1 (MQ=60)
                          TTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCG                                                                    >  SRR4302540.1292868/1‑94 (MQ=60)
                          TTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCG                                                                    >  SRR4302540.5694820/1‑94 (MQ=60)
                          TTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTACCTGAAGCCAGGCTTGCTCGGCACCGACCGATCCG                                                                    >  SRR4302540.7091258/1‑94 (MQ=60)
                           TTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGCCACAGACCGATGCGG                                                                   >  SRR4302540.3256525/1‑94 (MQ=60)
                            TTACGTCTTTACTGTCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGG                                                                  <  SRR4302540.9950270/94‑1 (MQ=60)
                            TTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGG                                                                  <  SRR4302540.4670277/94‑1 (MQ=60)
                            TTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGG                                                                  >  SRR4302540.4324962/1‑94 (MQ=60)
                            TTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCAAAGAACGATCCGGG                                                                  >  SRR4302540.9284823/1‑94 (MQ=60)
                              ACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGA                                                                >  SRR4302540.5399858/1‑94 (MQ=60)
                              ACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTAATTATTATGTCACGACGCTCCATGTAGCCAGGGTTGCCCGGCACAGACCGATCCGGCGA                                                                >  SRR4302540.5399857/1‑94 (MQ=60)
                               CGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGGA                                                               >  SRR4302540.5750034/1‑94 (MQ=60)
                               CGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAA                                                               <  SRR4302540.2255708/94‑1 (MQ=60)
                                GTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAA                                                              >  SRR4302540.5726233/1‑94 (MQ=60)
                                GTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAA                                                              <  SRR4302540.6700671/94‑1 (MQ=60)
                                GTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAA                                                              <  SRR4302540.1063630/94‑1 (MQ=60)
                                GTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAA                                                              >  SRR4302540.1588221/1‑94 (MQ=60)
                                GTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAA                                                              <  SRR4302540.9645155/94‑1 (MQ=60)
                                  CTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATT                                                            >  SRR4302540.150996/1‑94 (MQ=60)
                                  CTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATT                                                            >  SRR4302540.5803041/1‑94 (MQ=60)
                                   TTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTT                                                           <  SRR4302540.6513645/94‑1 (MQ=60)
                                      TCTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATT                                                        >  SRR4302540.2998658/1‑94 (MQ=60)
                                      ACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATT                                                        >  SRR4302540.8087510/1‑94 (MQ=60)
                                      ACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATT                                                        >  SRR4302540.3833210/1‑94 (MQ=60)
                                      ACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAACCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATT                                                        <  SRR4302540.8700125/94‑1 (MQ=60)
                                      ACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGGCACGACGCTCCCTGAAACCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATT                                                        <  SRR4302540.8700124/94‑1 (MQ=60)
                                        TGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCG                                                      <  SRR4302540.3447444/94‑1 (MQ=60)
                                            GATTAAAGAAGCCTCTCGCTGTTTATTATGTCCCGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCA                                                  <  SRR4302540.4538278/94‑1 (MQ=60)
                                            GATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGCAATTTATTCGCTCA                                                  >  SRR4302540.2334007/1‑94 (MQ=60)
                                            GATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCA                                                  <  SRR4302540.1288763/94‑1 (MQ=60)
                                            GATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCA                                                  <  SRR4302540.5756574/94‑1 (MQ=60)
                                            GATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCA                                                  >  SRR4302540.9444131/1‑94 (MQ=60)
                                            GATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCA                                                  <  SRR4302540.4090999/94‑1 (MQ=60)
                                            GATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCA                                                  <  SRR4302540.10156152/94‑1 (MQ=60)
                                             ATTAAATAAACCTCTCGCTGTTTATTATGTCACGGCCCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAA                                                 <  SRR4302540.7039405/94‑1 (MQ=60)
                                             ATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAA                                                 >  SRR4302540.920341/1‑94 (MQ=60)
                                               TAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATC                                               <  SRR4302540.5500219/94‑1 (MQ=60)
                                               TAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATC                                               >  SRR4302540.2916905/1‑94 (MQ=60)
                                               TAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATC                                               <  SRR4302540.575152/94‑1 (MQ=60)
                                                AAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCT                                              <  SRR4302540.1803198/94‑1 (MQ=60)
                                                AAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCT                                              >  SRR4302540.1088254/1‑94 (MQ=60)
                                                AAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCT                                              <  SRR4302540.2175762/94‑1 (MQ=60)
                                                 AAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATATA                                             >  SRR4302540.19122/1‑94 (MQ=60)
                                                  AGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTAC                                            >  SRR4302540.7046998/1‑94 (MQ=60)
                                                  AGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTAC                                            <  SRR4302540.8045060/94‑1 (MQ=60)
                                                  AGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTAC                                            >  SRR4302540.8442511/1‑94 (MQ=60)
                                                   GAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACT                                           <  SRR4302540.6303827/94‑1 (MQ=60)
                                                   GAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACT                                           <  SRR4302540.7734739/94‑1 (MQ=60)
                                                   AAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACT                                           <  SRR4302540.3417019/94‑1 (MQ=60)
                                                     AGCCTCTCGCTTTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTT                                         <  SRR4302540.7918141/94‑1 (MQ=60)
                                                     AGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTT                                         >  SRR4302540.7879508/1‑94 (MQ=60)
                                                     AGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCCCCACAGACCGATCCGGGGAAATTTATTCCCTCAATCTCCTTT                                         >  SRR4302540.7879509/1‑94 (MQ=60)
                                                      GCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTC                                        <  SRR4302540.4854089/94‑1 (MQ=60)
                                                       CCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCT                                       >  SRR4302540.2334993/1‑94 (MQ=60)
                                                       CCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCG                                       >  SRR4302540.1456291/1‑94 (MQ=60)
                                                        CTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGT                                      >  SRR4302540.6416511/1‑94 (MQ=60)
                                                        CTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGT                                      >  SRR4302540.5069121/1‑94 (MQ=60)
                                                         TCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTA                                     <  SRR4302540.5714596/94‑1 (MQ=60)
                                                         TCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTA                                     <  SRR4302540.3633477/94‑1 (MQ=60)
                                                         TCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTA                                     <  SRR4302540.3633479/94‑1 (MQ=60)
                                                         CCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTA                                     <  SRR4302540.5283154/94‑1 (MQ=60)
                                                          CTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAA                                    >  SRR4302540.8214649/1‑94 (MQ=60)
                                                          CTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAA                                    >  SRR4302540.1098676/1‑94 (MQ=60)
                                                          CTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAA                                    <  SRR4302540.9033536/94‑1 (MQ=60)
                                                              CTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTT                                >  SRR4302540.4708017/1‑94 (MQ=60)
                                                              CTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTT                                >  SRR4302540.3689217/1‑94 (MQ=60)
                                                               TGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTA                               >  SRR4302540.9469509/1‑94 (MQ=60)
                                                               TGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTA                               >  SRR4302540.4506913/1‑94 (MQ=60)
                                                                 TTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAA                             >  SRR4302540.9487587/1‑94 (MQ=60)
                                                                  TTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAG                            <  SRR4302540.58415/94‑1 (MQ=60)
                                                                  TTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAG                            <  SRR4302540.1807205/94‑1 (MQ=60)
                                                                  TTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAG                            <  SRR4302540.6261478/94‑1 (MQ=60)
                                                                   TATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGG                           >  SRR4302540.3589690/1‑94 (MQ=60)
                                                                   TATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGG                           >  SRR4302540.4041183/1‑94 (MQ=60)
                                                                   TATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGG                           >  SRR4302540.637517/1‑94 (MQ=60)
                                                                    ATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGC                          <  SRR4302540.8671033/94‑1 (MQ=60)
                                                                    ATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGC                          >  SRR4302540.7694139/1‑94 (MQ=60)
                                                                     TTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCG                         <  SRR4302540.8094443/94‑1 (MQ=60)
                                                                     TTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCG                         <  SRR4302540.4513864/94‑1 (MQ=60)
                                                                      TATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGC                        >  SRR4302540.7382660/1‑94 (MQ=60)
                                                                       ATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCT                       <  SRR4302540.1700584/94‑1 (MQ=60)
                                                                       ATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCT                       >  SRR4302540.5056357/1‑94 (MQ=60)
                                                                       ATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCT                       <  SRR4302540.3684806/94‑1 (MQ=60)
                                                                        TGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTG                      >  SRR4302540.6629503/1‑94 (MQ=60)
                                                                        TGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTG                      >  SRR4302540.6905297/1‑94 (MQ=60)
                                                                         GTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGC                     >  SRR4302540.4878561/1‑94 (MQ=60)
                                                                         GTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGC                     >  SRR4302540.6784259/1‑94 (MQ=60)
                                                                          TCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCC                    >  SRR4302540.3702731/1‑94 (MQ=60)
                                                                          TCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCC                    <  SRR4302540.3966847/94‑1 (MQ=60)
                                                                          TCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTAGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCC                    <  SRR4302540.5898989/94‑1 (MQ=60)
                                                                           CACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCG                   >  SRR4302540.3290477/1‑94 (MQ=60)
                                                                               ACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGAC               <  SRR4302540.192188/94‑1 (MQ=60)
                                                                               ACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGAA               >  SRR4302540.8466221/1‑94 (MQ=60)
                                                                                CGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACA              <  SRR4302540.2122851/94‑1 (MQ=60)
                                                                                CGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACA              <  SRR4302540.4794583/94‑1 (MQ=60)
                                                                                   TCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATT           <  SRR4302540.5310930/94‑1 (MQ=60)
                                                                                   TCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCCGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATT           >  SRR4302540.5522264/1‑94 (MQ=60)
                                                                                    CCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTC          >  SRR4302540.9676194/1‑94 (MQ=60)
                                                                                    CCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTC          >  SRR4302540.7711083/1‑94 (MQ=60)
                                                                                    CCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTC          >  SRR4302540.4867919/1‑94 (MQ=60)
                                                                                    CCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTGTCGGAATTTTAAAGGCGCTGCCGAGACAATTC          >  SRR4302540.7711084/1‑94 (MQ=60)
                                                                                     CCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCG         <  SRR4302540.4181440/94‑1 (MQ=60)
                                                                                     CCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCG         <  SRR4302540.8959077/94‑1 (MQ=60)
                                                                                      CTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGC        <  SRR4302540.7048146/94‑1 (MQ=60)
                                                                                      CTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGC        <  SRR4302540.7048319/94‑1 (MQ=60)
                                                                                      CTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGC        <  SRR4302540.8277683/94‑1 (MQ=60)
                                                                                      CTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGC        >  SRR4302540.1151415/1‑94 (MQ=60)
                                                                                      CTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGC        >  SRR4302540.1151416/1‑94 (MQ=60)
                                                                                       TGTACTCAGGAGTGCCGGGCACAGACCGAGCCGGGGAAAGTTATTCGCTCCATCTCCTTTCGGAATTGTAAAGGCGCTGCCGAGACAATTCGCG       <  SRR4302540.4816412/94‑1 (MQ=48)
                                                                                        GTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGA      >  SRR4302540.4878719/1‑94 (MQ=60)
                                                                                         TAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAA     >  SRR4302540.10114400/1‑94 (MQ=60)
                                                                                          AGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAA    <  SRR4302540.8907773/94‑1 (MQ=60)
                                                                                            CCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACCTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAAT  >  SRR4302540.609082/1‑94 (MQ=60)
                                                                                            |                                                                                             
AATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAAT  >  NC_000913/2232065‑2232250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑