Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,254,317 T→C T347A (ACG→GCG)  yeiM ← putative transport system permease protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,254,3170TC100.0% 835.1 / NA 261T347A (ACG→GCG) yeiMputative transport system permease protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (103/158);  total (103/158)

Only 100 of 278 total aligned reads displayed.

AAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGTGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGAT  >  NC_000913/2254227‑2254406
                                                                                          |                                                                                         
aaaaTGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAgcgcgc                                                                                         >  2:2938554/1‑93 (MQ=255)
aaaaTGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAgcgcgc                                                                                         >  1:1539837/1‑93 (MQ=255)
aaaaTGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAgcgcgc                                                                                         <  1:52840/93‑1 (MQ=255)
aaaaTGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAgcgcgc                                                                                         <  2:4027707/93‑1 (MQ=255)
 aaaTGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCgcc                                                                                        <  2:5069240/93‑1 (MQ=255)
  aaTGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCgccg                                                                                       >  2:6670569/1‑93 (MQ=255)
   aTGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGcgccgc                                                                                      >  2:2601912/1‑93 (MQ=255)
    tGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCgccgcc                                                                                     >  1:1079585/1‑93 (MQ=255)
    tGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCgccgcc                                                                                     <  1:5833216/93‑1 (MQ=255)
     gctccAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGccc                                                                                    <  2:908448/90‑1 (MQ=255)
         cAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGttt                                                                                <  2:6736420/93‑1 (MQ=255)
           aCGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGc                                                                              >  1:4286214/1‑93 (MQ=255)
            cGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCa                                                                             >  2:7875059/1‑93 (MQ=255)
             gACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAg                                                                            >  1:5412035/1‑93 (MQ=255)
              acaacaCCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAgg                                                                           >  1:3538407/1‑93 (MQ=255)
              acaacaCCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAgg                                                                           <  2:287429/93‑1 (MQ=255)
              acaacaCCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAgg                                                                           <  1:3968133/93‑1 (MQ=255)
                aacaCCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTa                                                                         <  2:7466891/93‑1 (MQ=255)
                 acacCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTAt                                                                        <  2:552562/93‑1 (MQ=255)
                 acacCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTAt                                                                        >  1:1163431/1‑93 (MQ=255)
                 acacCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTAt                                                                        >  1:6134518/1‑93 (MQ=255)
                  cacCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATg                                                                       <  2:5941396/93‑1 (MQ=255)
                  cacCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATg                                                                       <  2:1449006/93‑1 (MQ=255)
                   acCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATgg                                                                      <  2:8761963/93‑1 (MQ=255)
                    ccGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATggg                                                                     >  1:8607801/1‑93 (MQ=255)
                     cGATAGAACCAAAGTTAGCAAACCCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATgggg                                                                    <  2:8641352/93‑1 (MQ=255)
                      gATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGga                                                                   >  1:5161381/1‑93 (MQ=255)
                      gATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGga                                                                   >  2:3913491/1‑93 (MQ=255)
                      gATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGga                                                                   >  1:2620300/1‑93 (MQ=255)
                      gATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGCga                                                                   >  1:8485836/1‑93 (MQ=255)
                       aTAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGgag                                                                  >  1:6981580/1‑93 (MQ=255)
                           aaCCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAAc                                                              <  2:6043141/93‑1 (MQ=255)
                            aCCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTGACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGCAACt                                                             >  1:8886037/1‑93 (MQ=255)
                              cAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCa                                                           <  2:9396830/93‑1 (MQ=255)
                              cAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGATTGCAGGTATGGGGAGAAACTCa                                                           <  2:5424587/93‑1 (MQ=255)
                              cAAAGTTAGCAAAAACACAAAAACCAAAGGAGAAAATAGCAATGGTTTTTACTTCCATCGCCCCGCCCCCCTGCCGGTATGGGGGGAGACTCa                                                           >  2:9776755/1‑93 (MQ=255)
                                aaGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAgg                                                         <  1:1855030/93‑1 (MQ=255)
                                aaGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAgg                                                         >  1:8527485/1‑93 (MQ=255)
                                  gTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCTGTTTGCAGGTATGGGGAGAAACTCAGGTa                                                       <  1:4405623/93‑1 (MQ=255)
                                  gTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAGCTCAGGTa                                                       >  2:6376004/1‑93 (MQ=255)
                                  gTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTa                                                       <  1:9594525/93‑1 (MQ=255)
                                  gTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTa                                                       <  1:7906887/93‑1 (MQ=255)
                                   ttAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTaa                                                      <  2:4338411/93‑1 (MQ=255)
                                   ttAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTaa                                                      <  1:8002325/93‑1 (MQ=255)
                                   ttAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTaa                                                      >  2:264765/1‑93 (MQ=255)
                                     aGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGc                                                    >  1:2093706/1‑93 (MQ=255)
                                      gCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGcg                                                   <  2:6781503/93‑1 (MQ=255)
                                       cAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGcga                                                  <  2:7574555/93‑1 (MQ=255)
                                       cAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGcga                                                  <  2:7240639/93‑1 (MQ=255)
                                        aaaaCCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGcgac                                                 >  1:940833/1‑93 (MQ=255)
                                        aaaaCCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGcgac                                                 <  2:8423056/93‑1 (MQ=255)
                                         aaaCCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGcgacg                                                >  1:1693740/1‑93 (MQ=255)
                                          aaCCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGcgacga                                               <  1:8362351/93‑1 (MQ=255)
                                          aaCCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGcgacga                                               <  1:1559106/93‑1 (MQ=255)
                                           aCCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGaa                                              <  1:8690851/93‑1 (MQ=255)
                                             cacaAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAAtt                                            <  2:1147941/93‑1 (MQ=255)
                                              acaAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTc                                           <  2:9647636/93‑1 (MQ=255)
                                              acaAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTc                                           <  2:4844630/93‑1 (MQ=255)
                                              acaAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTc                                           >  1:6633510/1‑93 (MQ=255)
                                              acaAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTc                                           <  2:4575379/93‑1 (MQ=255)
                                                aaaGCGAAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGt                                         <  2:1751700/93‑1 (MQ=255)
                                                 aaGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGtt                                        >  2:5235587/1‑93 (MQ=255)
                                                  aGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTa                                       <  1:6308266/93‑1 (MQ=255)
                                                   gcgcAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTaa                                      <  1:3428653/93‑1 (MQ=255)
                                                   gcgcAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTaa                                      >  2:2257251/1‑93 (MQ=255)
                                                   gcgcAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTaa                                      <  2:5633413/93‑1 (MQ=255)
                                                     gcAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATc                                    >  1:4977194/1‑93 (MQ=255)
                                                     gcAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATc                                    >  1:3303594/1‑93 (MQ=255)
                                                     gcAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATc                                    <  1:9730426/93‑1 (MQ=255)
                                                      cAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGC‑GGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGc                                  <  2:1884070/93‑1 (MQ=255)
                                                       aaaGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGc                                  <  2:4174276/93‑1 (MQ=255)
                                                       aaaGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGc                                  >  2:8596150/1‑93 (MQ=255)
                                                        aaGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGAGACGAATTCGTTAATCGcc                                 >  2:2757663/1‑93 (MQ=255)
                                                          ggAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAg                               <  2:899897/93‑1 (MQ=255)
                                                             gaTAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGttt                            >  2:6506877/1‑93 (MQ=255)
                                                             gaTAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGttt                            >  2:1503670/1‑93 (MQ=255)
                                                              aTAATGGCATTGGTTGTCACTTCCAGCCCGCCGCCCGTTTGCAGGTATGGGGAGAAGCTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTc                           <  1:3913266/93‑1 (MQ=255)
                                                              aTAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTc                           <  2:1676525/93‑1 (MQ=255)
                                                              aTAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTc                           <  1:5986909/93‑1 (MQ=255)
                                                                  tCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGcc                       <  2:9521004/93‑1 (MQ=255)
                                                                   cGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGccc                      >  2:3582901/1‑93 (MQ=255)
                                                                   cGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGccc                      >  1:9235143/1‑93 (MQ=255)
                                                                      aaTGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAAt                   >  2:1929982/1‑93 (MQ=255)
                                                                      aaTGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAAt                   <  1:6348810/93‑1 (MQ=255)
                                                                      aaTGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAAt                   >  2:8151150/1‑93 (MQ=255)
                                                                        tGGTTTTCCCTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCa                 <  2:3328897/93‑1 (MQ=255)
                                                                        tGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCa                 >  2:7532614/1‑93 (MQ=255)
                                                                        tGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCa                 >  1:9837322/1‑93 (MQ=255)
                                                                        tGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCa                 <  1:8820420/93‑1 (MQ=255)
                                                                        tGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCa                 >  1:7270392/1‑93 (MQ=255)
                                                                          gTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAgg               <  2:469726/93‑1 (MQ=255)
                                                                           ttttCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGc              >  2:3424537/1‑93 (MQ=255)
                                                                           ttttCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGc              <  1:2793013/93‑1 (MQ=255)
                                                                            tttCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCt             <  1:1025517/93‑1 (MQ=255)
                                                                               cACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTAcc          <  1:7427128/93‑1 (MQ=255)
                                                                                aCTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTAccc         >  2:9944694/1‑93 (MQ=255)
                                                                                 cTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCg        >  1:2412219/1‑93 (MQ=255)
                                                                                    ccAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCaa     <  2:6185562/93‑1 (MQ=255)
                                                                                      aGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGa   >  1:4187167/1‑93 (MQ=255)
                                                                                       gcgcgcCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGAt  >  1:1256666/1‑93 (MQ=255)
                                                                                          |                                                                                         
AAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGTGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGAT  >  NC_000913/2254227‑2254406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGAAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGTGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGATTGGC  >  NC_000913/2254224‑2254410
                                                                                             |                                                                                             
CCGAAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGC                                                                                               <  SRR4302540.7002943/94‑1 (MQ=60)
CCGAAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGATTTCACTTCCAGCGC                                                                                               >  SRR4302540.9968538/1‑94 (MQ=60)
 CGAAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCG                                                                                              >  SRR4302540.721367/1‑94 (MQ=60)
 CGAAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCG                                                                                              >  SRR4302540.2271499/1‑94 (MQ=60)
  GAAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGC                                                                                             >  SRR4302540.1556859/1‑94 (MQ=60)
   AAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCC                                                                                            <  SRR4302540.53367/94‑1 (MQ=60)
    AAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCG                                                                                           >  SRR4302540.899050/1‑94 (MQ=60)
    AAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCG                                                                                           <  SRR4302540.287322/94‑1 (MQ=60)
      ATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCC                                                                                         >  SRR4302540.1090629/1‑94 (MQ=60)
      ATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCC                                                                                         >  SRR4302540.7067324/1‑94 (MQ=60)
       TGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCC                                                                                        <  SRR4302540.5897720/94‑1 (MQ=60)
       GGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCC                                                                                        <  SRR4302540.601735/94‑1 (MQ=60)
       AGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCC                                                                                        <  SRR4302540.1072146/94‑1 (MQ=60)
        GCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCG                                                                                       <  SRR4302540.5113515/94‑1 (MQ=60)
          GCCAACGACAACACCGATAGAACCAAAGTTAGCAAAGCCACAAATCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTT                                                                                     <  SRR4302540.8849133/94‑1 (MQ=60)
          GCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTT                                                                                     >  SRR4302540.715402/1‑94 (MQ=60)
           CCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTT                                                                                    <  SRR4302540.8905545/94‑1 (MQ=60)
           CCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTT                                                                                    >  SRR4302540.7423043/1‑94 (MQ=60)
           CCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTT                                                                                    <  SRR4302540.1395512/94‑1 (MQ=60)
           CCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTT                                                                                    >  SRR4302540.2736555/1‑94 (MQ=60)
           CCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGCTTTCACTTCCAGCGCGCCGCCCGTTT                                                                                    >  SRR4302540.2027772/1‑94 (MQ=60)
            CAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTG                                                                                   >  SRR4302540.1189996/1‑94 (MQ=60)
             AACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGC                                                                                  >  SRR4302540.4332840/1‑94 (MQ=60)
               CGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAG                                                                                >  SRR4302540.5471538/1‑94 (MQ=60)
               CGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAG                                                                                >  SRR4302540.5506205/1‑94 (MQ=60)
               CGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAG                                                                                <  SRR4302540.4155545/94‑1 (MQ=60)
                GACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGG                                                                               >  SRR4302540.9516388/1‑94 (MQ=60)
                GACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGG                                                                               >  SRR4302540.3577041/1‑94 (MQ=60)
                 ACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGT                                                                              <  SRR4302540.4010911/94‑1 (MQ=60)
                   AACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTAT                                                                            >  SRR4302540.5685446/1‑94 (MQ=60)
                   AACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTAT                                                                            <  SRR4302540.3901403/94‑1 (MQ=60)
                   AACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTAT                                                                            >  SRR4302540.1175508/1‑94 (MQ=60)
                   AACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTAT                                                                            >  SRR4302540.6202844/1‑94 (MQ=60)
                   AACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTAT                                                                            <  SRR4302540.6418255/94‑1 (MQ=60)
                   AACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTAT                                                                            <  SRR4302540.9405425/94‑1 (MQ=60)
                    ACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATG                                                                           <  SRR4302540.95298/94‑1 (MQ=60)
                    ACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATG                                                                           <  SRR4302540.6827178/94‑1 (MQ=60)
                      ACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGG                                                                         <  SRR4302540.3441312/94‑1 (MQ=60)
                      ACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGG                                                                         >  SRR4302540.8707827/1‑94 (MQ=60)
                        CGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGA                                                                       >  SRR4302540.5217492/1‑94 (MQ=60)
                        CGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGA                                                                       >  SRR4302540.2648624/1‑94 (MQ=60)
                        CGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGA                                                                       >  SRR4302540.7005859/1‑94 (MQ=60)
                        CGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGCGA                                                                       >  SRR4302540.8584419/1‑94 (MQ=60)
                         GATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAG                                                                      >  SRR4302540.7059130/1‑94 (MQ=60)
                          ATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGA                                                                     <  SRR4302540.4253369/94‑1 (MQ=60)
                          ATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGA                                                                     >  SRR4302540.2494221/1‑94 (MQ=60)
                            AGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAA                                                                   >  SRR4302540.8190067/1‑94 (MQ=60)
                            AGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAA                                                                   <  SRR4302540.9882335/94‑1 (MQ=60)
                             GAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAAC                                                                  >  SRR4302540.8033131/1‑94 (MQ=60)
                             GAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGCGAGAAAC                                                                  >  SRR4302540.2377463/1‑94 (MQ=60)
                              AACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTGACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGCAACT                                                                 >  SRR4302540.8988896/1‑94 (MQ=60)
                               ACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTC                                                                >  SRR4302540.3444195/1‑94 (MQ=60)
                                 CAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAG                                                              <  SRR4302540.4338090/94‑1 (MQ=60)
                                  AAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGG                                                             >  SRR4302540.8626568/1‑94 (MQ=60)
                                  AAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCCCGCCGCCCGTTTGCAGGTATGGGGAGAAAATCAGG                                                             >  SRR4302540.8626569/1‑94 (MQ=60)
                                   AAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGT                                                            <  SRR4302540.1875374/94‑1 (MQ=60)
                                   AAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGT                                                            >  SRR4302540.2069909/1‑94 (MQ=60)
                                     GTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCTGTTTGCAGGTATGGGGAGAAACTCAGGTAA                                                          <  SRR4302540.4453387/94‑1 (MQ=60)
                                     GTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAA                                                          <  SRR4302540.7979452/94‑1 (MQ=60)
                                     GTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAA                                                          <  SRR4302540.7999095/94‑1 (MQ=60)
                                     GTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAA                                                          <  SRR4302540.9707749/94‑1 (MQ=60)
                                     GTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAA                                                          <  SRR4302540.1202373/94‑1 (MQ=60)
                                     GTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAA                                                          <  SRR4302540.9707748/94‑1 (MQ=60)
                                     GTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAA                                                          >  SRR4302540.8489360/1‑94 (MQ=60)
                                      TTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAG                                                         <  SRR4302540.8095461/94‑1 (MQ=60)
                                       TAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGC                                                        >  SRR4302540.2116186/1‑94 (MQ=60)
                                        AGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCG                                                       <  SRR4302540.5221419/94‑1 (MQ=60)
                                          CAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGAC                                                     <  SRR4302540.1575774/94‑1 (MQ=60)
                                          CAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGAC                                                     <  SRR4302540.3492845/94‑1 (MQ=60)
                                          CAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGAC                                                     >  SRR4302540.950665/1‑94 (MQ=60)
                                           AAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACG                                                    >  SRR4302540.1712104/1‑94 (MQ=60)
                                             AACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAA                                                  <  SRR4302540.1576232/94‑1 (MQ=60)
                                             AACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAA                                                  <  SRR4302540.8459393/94‑1 (MQ=60)
                                             AACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAA                                                  <  SRR4302540.8777136/94‑1 (MQ=60)
                                              ACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAAT                                                 <  SRR4302540.6434304/94‑1 (MQ=60)
                                              ACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAAT                                                 <  SRR4302540.6568190/94‑1 (MQ=60)
                                              ACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAAT                                                 <  SRR4302540.8791752/94‑1 (MQ=60)
                                                CACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTC                                               >  SRR4302540.6706927/1‑94 (MQ=60)
                                                CACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTC                                               <  SRR4302540.4256682/94‑1 (MQ=60)
                                                  CAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGT                                             <  SRR4302540.3143879/94‑1 (MQ=60)
                                                    AAGAGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAAACCAGGTAAGCGACGAATTCGTTA                                           <  SRR4302540.1822893/94‑1 (MQ=60)
                                                     AGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAA                                          <  SRR4302540.5381646/94‑1 (MQ=60)
                                                     AGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAA                                          <  SRR4302540.6378514/94‑1 (MQ=60)
                                                      GCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAAT                                         <  SRR4302540.4470072/94‑1 (MQ=60)
                                                      GCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAAT                                         <  SRR4302540.3465911/94‑1 (MQ=60)
                                                       CGCAAAGGAGATAATCGCAATGTTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATC                                        <  SRR4302540.16765/94‑1 (MQ=60)
                                                       CGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATC                                        >  SRR4302540.3339767/1‑94 (MQ=60)
                                                       CGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATC                                        >  SRR4302540.1321105/1‑94 (MQ=60)
                                                       CGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATC                                        >  SRR4302540.5031065/1‑94 (MQ=60)
                                                       CGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATC                                        >  SRR4302540.6324649/1‑94 (MQ=60)
                                                        GCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCG                                       <  SRR4302540.9844686/94‑1 (MQ=60)
                                                         CAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAATTCAGGgacgggcgaaattctgtagagca                                      >  SRR4302540.270123/1‑71 (MQ=60)
                                                         CAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGC                                      >  SRR4302540.4942803/1‑94 (MQ=60)
                                                          AAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCC                                     >  SRR4302540.8579697/1‑94 (MQ=60)
                                                          AAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCC                                     >  SRR4302540.1138927/1‑94 (MQ=60)
                                                          AAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCC                                     >  SRR4302540.1744384/1‑94 (MQ=60)
                                                          AAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCC                                     <  SRR4302540.8609913/94‑1 (MQ=60)
                                                           AAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCA                                    <  SRR4302540.9624988/94‑1 (MQ=60)
                                                             GGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGT                                  <  SRR4302540.8109945/94‑1 (MQ=60)
                                                               AGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTT                                >  SRR4302540.9756624/1‑94 (MQ=60)
                                                                GATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTC                               <  SRR4302540.8791203/94‑1 (MQ=60)
                                                                GATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTC                               <  SRR4302540.1119671/94‑1 (MQ=60)
                                                                 ATACTCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGTGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCT                              <  SRR4302540.6064449/94‑1 (MQ=60)
                                                                 ATAATGGCATTGGTTGTCACTTCCAGCCCGCCGCCCGTTTGCAGGTATGGGGAGAAGCTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCT                              <  SRR4302540.3955661/94‑1 (MQ=60)
                                                                 ATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCT                              <  SRR4302540.6064451/94‑1 (MQ=60)
                                                                 ATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCT                              <  SRR4302540.6053219/94‑1 (MQ=60)
                                                                 ATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCT                              <  SRR4302540.6053218/94‑1 (MQ=60)
                                                                  TAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTG                             <  SRR4302540.8677189/94‑1 (MQ=60)
                                                                  TAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTC                             >  SRR4302540.4009800/1‑94 (MQ=60)
                                                                  TAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAACCGACGAATTCGTTACTCGCCAGTTTCTG                             >  SRR4302540.6359126/1‑94 (MQ=60)
                                                                    ATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTTCC                           >  SRR4302540.8349306/1‑94 (MQ=60)
                                                                     TCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCC                          >  SRR4302540.9345803/1‑94 (MQ=60)
                                                                      CGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCA                         >  SRR4302540.1634499/1‑94 (MQ=60)
                                                                         AATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAATTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTTCCCAATC                      >  SRR4302540.9029239/1‑94 (MQ=60)
                                                                         AATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATC                      >  SRR4302540.8047609/1‑94 (MQ=60)
                                                                         AATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATC                      >  SRR4302540.2721405/1‑94 (MQ=60)
                                                                         AATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATC                      <  SRR4302540.6419512/94‑1 (MQ=60)
                                                                          ATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCA                     >  SRR4302540.9952348/1‑94 (MQ=60)
                                                                          ATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCA                     >  SRR4302540.3998147/1‑94 (MQ=60)
                                                                          ATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCA                     >  SRR4302540.7351519/1‑94 (MQ=60)
                                                                           TGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAG                    <  SRR4302540.8922410/94‑1 (MQ=60)
                                                                            GGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGG                   >  SRR4302540.2160231/1‑94 (MQ=60)
                                                                             GTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGC                  >  SRR4302540.8983137/1‑94 (MQ=60)
                                                                              TTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCT                 <  SRR4302540.5241327/94‑1 (MQ=60)
                                                                              TTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCT                 <  SRR4302540.2823165/94‑1 (MQ=60)
                                                                               TTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTA                <  SRR4302540.1035918/94‑1 (MQ=60)
                                                                                TTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTAC               <  SRR4302540.4191060/94‑1 (MQ=60)
                                                                                  CACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCC             <  SRR4302540.7509867/94‑1 (MQ=60)
                                                                                   ACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCG            >  SRR4302540.2438221/1‑94 (MQ=60)
                                                                                       CCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAG        <  SRR4302540.5137518/94‑1 (MQ=60)
                                                                                       CCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAG        <  SRR4302540.3792450/94‑1 (MQ=60)
                                                                                        CAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGA       >  SRR4302540.4232687/1‑94 (MQ=60)
                                                                                         AGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGAT      >  SRR4302540.1269547/1‑94 (MQ=60)
                                                                                          GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGATT     <  SRR4302540.783719/94‑1 (MQ=60)
                                                                                           CGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGATTG    >  SRR4302540.1643371/1‑94 (MQ=60)
                                                                                             CGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGATTGGC  >  SRR4302540.4467824/1‑94 (MQ=60)
                                                                                             |                                                                                             
CCGAAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGTGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGATTGGC  >  NC_000913/2254224‑2254410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑