Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,259,919	C→T	R156H (CGC→CAC)	fruK ←	fructose‑1‑phosphate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,259,919	0	C	T	99.6%	962.3 / NA	273	R156H (CGC→CAC)	fruK	fructose‑1‑phosphate kinase
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (132/140); minor base G (0/1); total (132/141)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 282 total aligned reads displayed.

GGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTC  >  NC_000913/2259829‑2260008

ggTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGa                                                                                         <  1:4482941/93‑1 (MQ=255)

 gTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAg                                                                                        <  1:7256433/93‑1 (MQ=255)

 gTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAg                                                                                        >  2:2637032/1‑93 (MQ=255)

  tttCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGt                                                                                       >  2:9076707/1‑93 (MQ=255)

   ttCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTc                                                                                      <  2:371804/93‑1 (MQ=255)

     cACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCAt                                                                                    <  2:1316659/93‑1 (MQ=255)

     cACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCAt                                                                                    >  1:6058579/1‑93 (MQ=255)

      aCCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATc                                                                                   <  2:3561197/93‑1 (MQ=255)

       ccAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATcc                                                                                  <  2:948794/93‑1 (MQ=255)

        cAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCa                                                                                 <  2:9221524/93‑1 (MQ=255)

        cAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCa                                                                                 <  2:9281277/93‑1 (MQ=255)

         cgCCACGCGGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAg                                                                                <  1:5124485/92‑1 (MQ=255)

         agacACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAg                                                                                >  2:6189031/4‑93 (MQ=255)

            cACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCg                                                                             >  1:9366738/1‑93 (MQ=255)

             aCGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCgg                                                                            >  1:9750327/1‑93 (MQ=255)

               ggTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTg                                                                          <  2:8078029/93‑1 (MQ=255)

                 tGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGaa                                                                        >  2:1512223/1‑93 (MQ=255)

                  gCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAAc                                                                       >  1:1207537/1‑93 (MQ=255)

                  gCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAAc                                                                       >  2:6474784/1‑93 (MQ=255)

                  gCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAAc                                                                       >  1:8764406/1‑93 (MQ=255)

                  gCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAAc                                                                       >  1:4809812/1‑93 (MQ=255)

                  gCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAAc                                                                       <  2:8834645/93‑1 (MQ=255)

                   ccGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACg                                                                      >  1:6822099/1‑93 (MQ=255)

                    cGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGc                                                                     <  2:8440223/93‑1 (MQ=255)

                      cTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCtt                                                                   <  1:9262867/93‑1 (MQ=255)

                      cTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCtt                                                                   <  2:8644900/93‑1 (MQ=255)

                       tttCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTc                                                                  <  1:10039825/93‑1 (MQ=255)

                        ttCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTcc                                                                 >  2:8200571/1‑93 (MQ=255)

                         tCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCg                                                                >  1:2023144/1‑93 (MQ=255)

                         tCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCg                                                                <  1:7383608/93‑1 (MQ=255)

                          cAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCgg                                                               >  1:8383517/1‑93 (MQ=255)

                           aaaCCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCggc                                                              >  1:4149655/1‑92 (MQ=255)

                               cTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGa                                                          <  1:9516615/93‑1 (MQ=255)

                               cTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGa                                                          <  2:1950924/93‑1 (MQ=255)

                               cTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGa                                                          <  2:6035372/93‑1 (MQ=255)

                               cTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGa                                                          <  1:3344696/93‑1 (MQ=255)

                                tGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGAc                                                         >  1:6170394/1‑93 (MQ=255)

                                tGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGAc                                                         <  2:1071924/93‑1 (MQ=255)

                                tGCTACTAACGCTTCACGGCTACTAACAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGAc                                                         <  2:2557770/93‑1 (MQ=255)

                                  ctactaACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGc                                                       >  2:2228990/1‑93 (MQ=255)

                                   tactaACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGcc                                                      >  1:3715690/1‑93 (MQ=255)

                                   tactaACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGcc                                                      >  1:16627/1‑93 (MQ=255)

                                   tactaACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGcc                                                      <  2:1268174/93‑1 (MQ=255)

                                    actaACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCt                                                     <  2:9788602/93‑1 (MQ=255)

                                    actaACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCt                                                     >  2:2125025/1‑93 (MQ=255)

                                      taACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTgc                                                   >  2:5026698/1‑92 (MQ=255)

                                      taACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGa                                                   >  2:426008/1‑93 (MQ=255)

                                      taACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGa                                                   <  1:807993/93‑1 (MQ=255)

                                      taACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAAAGCTTCCGGGCTGACGCCTGa                                                   >  2:7307975/1‑93 (MQ=255)

                                        aCGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACg                                                 <  2:7604718/93‑1 (MQ=255)

                                        aCGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGGCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACg                                                 >  2:7874655/1‑93 (MQ=255)

                                         cGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACgg                                                <  2:6627193/93‑1 (MQ=255)

                                          gCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGt                                               >  2:404183/1‑93 (MQ=255)

                                          gCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGt                                               >  1:1731234/1‑93 (MQ=255)

                                           cTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTa                                              <  2:1729782/93‑1 (MQ=255)

                                           cTTCACGGCTACTATCAAAGATAATGCAAGGACACGGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTa                                              >  1:1793866/1‑93 (MQ=255)

                                             tCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAg                                            >  1:5220692/1‑93 (MQ=255)

                                             tCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAg                                            >  2:5708904/1‑93 (MQ=255)

                                              cACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGc                                           <  2:2352965/93‑1 (MQ=255)

                                              cACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGc                                           <  2:4511448/93‑1 (MQ=255)

                                                 ggCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTc                                        <  2:1820747/93‑1 (MQ=255)

                                                 ggCTAATATCAAAGATAATGCCAGGACACTGAATACGCAGGTGAGTCATCCAGTAGGTGAACGCTTCCGGGCTGACGCCTGACGGTAACCTTc                                        <  2:9837831/93‑1 (MQ=255)

                                                  gCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTcc                                       >  2:8585190/1‑93 (MQ=255)

                                                   ctactaTCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCg                                      <  2:5867664/93‑1 (MQ=255)

                                                     actaTCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCt                                    <  2:6781151/93‑1 (MQ=255)

                                                     actaTCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCt                                    >  1:5770980/1‑93 (MQ=255)

                                                      ctaTCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTg                                   >  2:3014478/1‑93 (MQ=255)

                                                       taTCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGa                                  >  2:7017038/1‑93 (MQ=255)

                                                       taTCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGa                                  >  2:5947042/1‑93 (MQ=255)

                                                       taTCAAAGATAATCCAAGGACATTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCGGACGGTAAGCTTCCGCTGa                                  <  2:8845357/93‑1 (MQ=255)

                                                        aTCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGac                                 >  1:2859836/1‑93 (MQ=255)

                                                          cAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGacac                               <  2:625329/93‑1 (MQ=255)

                                                           aaaGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGAcaca                              <  1:2171804/93‑1 (MQ=255)

                                                           aaaGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGAcaca                              <  1:2261237/93‑1 (MQ=255)

                                                           aaaGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGAcaca                              >  1:5540073/1‑93 (MQ=255)

                                                              gATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGAc                           >  2:9125107/1‑93 (MQ=255)

                                                               aTAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGAcc                          <  1:4567268/93‑1 (MQ=255)

                                                                tAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCa                         <  1:7158565/93‑1 (MQ=255)

                                                                tAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCa                         <  2:3735136/93‑1 (MQ=255)

                                                                  aTGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCata                       <  1:5408102/93‑1 (MQ=255)

                                                                  aTGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCata                       <  1:2332162/93‑1 (MQ=255)

                                                                  aTGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCata                       <  1:3045994/93‑1 (MQ=255)

                                                                   tGCAAGGACACTGACTACGTAGGTGAGTCTTCCAGTCGGTGAACGCTTCCGGGCTGACGTCTGATGGGTTGCTTCCGCTGACACAGACCatat                      >  1:8960734/1‑93 (MQ=255)

                                                                   tGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCatat                      <  1:2723036/93‑1 (MQ=255)

                                                                   tGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCatat                      <  1:9618274/93‑1 (MQ=255)

                                                                    gCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATc                     <  2:5218394/93‑1 (MQ=255)

                                                                     cAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCg                    <  2:4784253/93‑1 (MQ=255)

                                                                       aGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGaa                  <  1:1353699/93‑1 (MQ=255)

                                                                       aGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGaa                  <  2:3856832/93‑1 (MQ=255)

                                                                          acacTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTg               <  1:5170315/93‑1 (MQ=255)

                                                                           cacTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACtac              >  1:64195/1‑91 (MQ=255)

                                                                           cacTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGa              >  1:3985280/1‑93 (MQ=255)

                                                                           cacTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGa              <  1:2410027/93‑1 (MQ=255)

                                                                             cTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGAcc            <  2:7170299/93‑1 (MQ=255)

                                                                               gACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGa          <  2:6416762/93‑1 (MQ=255)

                                                                                  tACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGcc       <  2:3416710/93‑1 (MQ=255)

                                                                                       aGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTc  <  2:5457500/93‑1 (MQ=255)

                                                                                       aGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTc  <  1:8651641/93‑1 (MQ=255)

                                                                                       aGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTc  >  1:7807013/1‑93 (MQ=255)

                                                                                       aGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGAACATATCGAACTGACCGAGCCAGCTc  >  1:1432547/1‑93 (MQ=255)

GGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTC  >  NC_000913/2259829‑2260008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAG  >  NC_000913/2259826‑2260010

TTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGT                                                                                             <  SRR4302540.5424092/94‑1 (MQ=60)

   GGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAG                                                                                          <  SRR4302540.4531559/94‑1 (MQ=60)

   GGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAG                                                                                          <  SRR4302540.225128/94‑1 (MQ=60)

    GTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGT                                                                                         <  SRR4302540.7337404/94‑1 (MQ=60)

     TTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTC                                                                                        <  SRR4302540.2344023/94‑1 (MQ=60)

     TTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTC                                                                                        >  SRR4302540.3174119/1‑94 (MQ=60)

       TCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCAT                                                                                      <  SRR4302540.9803152/94‑1 (MQ=60)

       TCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCAT                                                                                      >  SRR4302540.6125914/1‑94 (MQ=60)

        CACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATC                                                                                     >  SRR4302540.8489187/1‑94 (MQ=60)

          CCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCA                                                                                   <  SRR4302540.9579490/94‑1 (MQ=60)

           CAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAG                                                                                  <  SRR4302540.7749788/94‑1 (MQ=60)

           CAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAG                                                                                  <  SRR4302540.156405/94‑1 (MQ=60)

            cgccacgcgGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGT                                                                                 <  SRR4302540.5179987/85‑1 (MQ=60)

            AGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGT                                                                                 >  SRR4302540.238928/1‑94 (MQ=60)

              CCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCG                                                                               >  SRR4302540.9478268/1‑94 (MQ=60)

               CACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGG                                                                              >  SRR4302540.9864741/1‑94 (MQ=60)

               CACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGG                                                                              >  SRR4302540.3426413/1‑94 (MQ=60)

               CACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGG                                                                              >  SRR4302540.6179880/1‑94 (MQ=60)

                  GGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGA                                                                           <  SRR4302540.1400476/94‑1 (MQ=60)

                  GGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGA                                                                           >  SRR4302540.5580943/1‑94 (MQ=60)

                    TGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAAC                                                                         >  SRR4302540.8866010/1‑94 (MQ=60)

                    TGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAAC                                                                         >  SRR4302540.1219930/1‑94 (MQ=60)

                    TGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAAC                                                                         >  SRR4302540.4861292/1‑94 (MQ=60)

                    TGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAAC                                                                         >  SRR4302540.4861290/1‑94 (MQ=60)

                    TGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTAACTACGCAAGTGAGTCATACAGTCGGTGCAC                                                                         >  SRR4302540.4861289/1‑94 (MQ=60)

                     GCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACG                                                                        >  SRR4302540.6897626/1‑94 (MQ=60)

                     GCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACG                                                                        <  SRR4302540.7179250/94‑1 (MQ=60)

                        GCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTT                                                                     <  SRR4302540.7265447/94‑1 (MQ=60)

                         CTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTC                                                                    <  SRR4302540.9373670/94‑1 (MQ=60)

                         CTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTC                                                                    >  SRR4302540.824773/1‑94 (MQ=60)

                          TTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCC                                                                   <  SRR4302540.10156566/94‑1 (MQ=60)

                           TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCG                                                                  >  SRR4302540.2045008/1‑94 (MQ=60)

                           TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCG                                                                  <  SRR4302540.9058711/94‑1 (MQ=60)

                            TCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGG                                                                 <  SRR4302540.2330693/94‑1 (MQ=60)

                            TCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGG                                                                 <  SRR4302540.7465878/94‑1 (MQ=60)

                            TCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGG                                                                 >  SRR4302540.8480787/1‑94 (MQ=60)

                             CAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGC                                                                >  SRR4302540.4194584/1‑94 (MQ=60)

                               AACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCTTCCAGTCGGTGAACGCTTCCGGGCT                                                              <  SRR4302540.1336560/94‑1 (MQ=60)

                                ACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTG                                                             <  SRR4302540.9406241/94‑1 (MQ=60)

                                 CCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGA                                                            >  SRR4302540.5867050/1‑94 (MQ=60)

                                 CCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGA                                                            >  SRR4302540.2939859/1‑94 (MQ=60)

                                  CTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGAC                                                           >  SRR4302540.900574/1‑94 (MQ=60)

                                  CTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGAC                                                           <  SRR4302540.3381391/94‑1 (MQ=60)

                                  CTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGAC                                                           >  SRR4302540.6239139/1‑94 (MQ=60)

                                  CTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGAC                                                           >  SRR4302540.6239138/1‑94 (MQ=60)

                                  CTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGAC                                                           <  SRR4302540.9629324/94‑1 (MQ=60)

                                   TGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCAAGTCGGTGAACGCTTCCGGGCTGACG                                                          >  SRR4302540.6130866/1‑94 (MQ=60)

                                   CGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACGCTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACG                                                          <  SRR4302540.6648865/94‑1 (MQ=60)

                                     CTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCC                                                        >  SRR4302540.2389071/1‑94 (MQ=60)

                                     CTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCC                                                        >  SRR4302540.3756230/1‑94 (MQ=60)

                                     CTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCC                                                        >  SRR4302540.16810/1‑94 (MQ=60)

                                     CTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCC                                                        <  SRR4302540.314297/94‑1 (MQ=60)

                                        CTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACTCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGTCGCCTGA                                                     >  SRR4302540.113107/1‑94 (MQ=60)

                                        CTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGA                                                     >  SRR4302540.7611092/1‑94 (MQ=60)

                                        CTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGA                                                     >  SRR4302540.2698155/1‑94 (MQ=60)

                                        CTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACCGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGA                                                     >  SRR4302540.4071417/1‑94 (MQ=60)

                                         TAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGAC                                                    <  SRR4302540.816579/94‑1 (MQ=60)

                                          AACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACG                                                   >  SRR4302540.9636952/1‑94 (MQ=60)

                                          AACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACG                                                   <  SRR4302540.10032714/94‑1 (MQ=60)

                                            CGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGT                                                 >  SRR4302540.1749919/1‑94 (MQ=60)

                                            CGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCCGGCTGACGACTGACGGC                                                 >  SRR4302540.1749918/1‑94 (MQ=60)

                                             GCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGCCGCCTGACGGTA                                                >  SRR4302540.7301423/1‑94 (MQ=60)

                                             GCTTCACGGCTACTATCAAAGATAATGCAAGGACACGGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTA                                                >  SRR4302540.1813365/1‑94 (MQ=60)

                                               TTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAG                                              <  SRR4302540.4321119/94‑1 (MQ=60)

                                               TTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAG                                              >  SRR4302540.5552597/1‑94 (MQ=60)

                                               TTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAG                                              >  SRR4302540.5277610/1‑94 (MQ=60)

                                                TCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGC                                             <  SRR4302540.5432182/94‑1 (MQ=60)

                                                 CACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCT                                            >  SRR4302540.518175/1‑94 (MQ=60)

                                                     GCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCG                                        <  SRR4302540.6730728/94‑1 (MQ=60)

                                                     GCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCG                                        >  SRR4302540.5114090/1‑94 (MQ=60)

                                                       TACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCT                                      >  SRR4302540.5834716/1‑94 (MQ=60)

                                                       TACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCT                                      >  SRR4302540.4612471/1‑94 (MQ=60)

                                                        ACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTG                                     <  SRR4302540.6409746/94‑1 (MQ=60)

                                                        ACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTG                                     >  SRR4302540.5606244/1‑94 (MQ=60)

                                                         CTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGA                                    >  SRR4302540.5315615/1‑94 (MQ=60)

                                                          TATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGAC                                   >  SRR4302540.2890834/1‑94 (MQ=60)

                                                           ATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACA                                  >  SRR4302540.8307608/1‑94 (MQ=60)

                                                           ATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACA                                  >  SRR4302540.2881525/1‑94 (MQ=60)

                                                            TCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCTGGCTGCAGCCTGACGGTAAGCTTCCGCTCACAC                                 >  SRR4302540.5273157/1‑94 (MQ=60)

                                                            TCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACAC                                 <  SRR4302540.7995784/94‑1 (MQ=60)

                                                            TCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACAC                                 >  SRR4302540.5273154/1‑94 (MQ=60)

                                                             CAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACA                                >  SRR4302540.2010166/1‑94 (MQ=60)

                                                             CAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACA                                >  SRR4302540.5601093/1‑94 (MQ=60)

                                                              AAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAG                               <  SRR4302540.2285498/94‑1 (MQ=60)

                                                              AAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAG                               <  SRR4302540.2195222/94‑1 (MQ=60)

                                                                AGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCCGACACAGAC                             >  SRR4302540.2274152/1‑94 (MQ=60)

                                                                AGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCAGCTGACACAGAC                             >  SRR4302540.1515167/1‑94 (MQ=60)

                                                                 GATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACC                            <  SRR4302540.2931373/94‑1 (MQ=60)

                                                                  ATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCA                           >  SRR4302540.2082938/1‑94 (MQ=60)

                                                                  ATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCA                           <  SRR4302540.4616579/94‑1 (MQ=60)

                                                                  ATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCA                           >  SRR4302540.4004431/1‑94 (MQ=60)

                                                                   TAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCAT                          <  SRR4302540.7238351/94‑1 (MQ=60)

                                                                    AATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATA                         <  SRR4302540.7420536/94‑1 (MQ=60)

                                                                     ATGCAAGGACACTGACTACGTAGGTGAGTCTTCCAGTCGGTGAACGCTTCCGGGCTGACGTCTGATGGGTTGCTTCCGCTGACACAGACCATAT                        >  SRR4302540.9066744/1‑94 (MQ=60)

                                                                     ATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATAT                        >  SRR4302540.1968883/1‑94 (MQ=60)

                                                                     ATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATAT                        <  SRR4302540.2357292/94‑1 (MQ=60)

                                                                     ATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATAT                        <  SRR4302540.7024651/94‑1 (MQ=60)

                                                                     ATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATAT                        <  SRR4302540.5467571/94‑1 (MQ=60)

                                                                     ATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATAT                        <  SRR4302540.3079416/94‑1 (MQ=60)

                                                                      TGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATC                       <  SRR4302540.9731660/94‑1 (MQ=60)

                                                                      TGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATC                       <  SRR4302540.2752394/94‑1 (MQ=60)

                                                                      TGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATC                       >  SRR4302540.4353748/1‑94 (MQ=60)

                                                                      TGCAAGGACACTGACAACGCAGATAAGTCATCCGGTCGGTGAACGATTCCGGGCTGACGCATGACGGTAAGCTTCCGCTGACCCAGACCATAAC                       <  SRR4302540.9091100/94‑1 (MQ=60)

                                                                         AAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAA                    <  SRR4302540.9673849/94‑1 (MQ=60)

                                                                         AAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAA                    >  SRR4302540.2087707/1‑94 (MQ=60)

                                                                         AAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAA                    >  SRR4302540.1691508/1‑94 (MQ=60)

                                                                          AGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAAC                   <  SRR4302540.1367948/94‑1 (MQ=60)

                                                                          AGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACCCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAAC                   <  SRR4302540.5637141/94‑1 (MQ=60)

                                                                            GACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTG                 >  SRR4302540.9378114/1‑94 (MQ=60)

                                                                            GACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTG                 <  SRR4302540.135428/94‑1 (MQ=60)

                                                                             ACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTac                >  SRR4302540.64841/1‑92 (MQ=60)

                                                                             ACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGA                <  SRR4302540.5226568/94‑1 (MQ=60)

                                                                             ACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGA                <  SRR4302540.366440/94‑1 (MQ=60)

                                                                             ACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGA                >  SRR4302540.4028193/1‑94 (MQ=60)

                                                                              CACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGAC               <  SRR4302540.2436010/94‑1 (MQ=60)

                                                                               ACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACC              <  SRR4302540.9582367/94‑1 (MQ=60)

                                                                               ACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACC              <  SRR4302540.4060118/94‑1 (MQ=60)

                                                                                CTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCG             <  SRR4302540.1751167/94‑1 (MQ=60)

                                                                                CTGACTACGCAGGGGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCG             <  SRR4302540.4612234/94‑1 (MQ=60)

                                                                                    CTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCC         >  SRR4302540.7929078/1‑94 (MQ=60)

                                                                                    CTACGCAGGTGAGCCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGCCACACACCATATCGAACTGACCGAtcg         >  SRR4302540.9117922/1‑91 (MQ=60)

                                                                                     TACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCA        <  SRR4302540.1408089/94‑1 (MQ=60)

                                                                                        GCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCT     <  SRR4302540.5012561/94‑1 (MQ=60)

                                                                                        GCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCT     >  SRR4302540.2853726/1‑94 (MQ=60)

                                                                                         CAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTC    >  SRR4302540.7898242/1‑94 (MQ=60)

                                                                                         CAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGAACATATCGAACTGACCGAGCCAGCTC    >  SRR4302540.1448073/1‑94 (MQ=60)

                                                                                          AGGTGAGTCGTCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACGGACCGAGCCAGCTCA   <  SRR4302540.8752143/94‑1 (MQ=60)

                                                                                          AGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCA   >  SRR4302540.5313326/1‑94 (MQ=60)

                                                                                          AGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCA   <  SRR4302540.8752144/94‑1 (MQ=60)

                                                                                          AGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACCCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCA   <  SRR4302540.8752145/94‑1 (MQ=60)

                                                                                           GGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCCTATCGAACTGACCGAGCAAGCTCAG  >  SRR4302540.8030035/1‑94 (MQ=60)

                                                                                           GGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAG  >  SRR4302540.3383256/1‑94 (MQ=60)

                                                                                           GGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAG  >  SRR4302540.9956477/1‑94 (MQ=60)

                                                                                           GGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAG  <  SRR4302540.2028478/94‑1 (MQ=60)

                                                                                           GGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAG  >  SRR4302540.2046562/1‑94 (MQ=60)

                                                                                           GGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAG  <  SRR4302540.7059535/94‑1 (MQ=60)

                                                                                           GGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAG  <  SRR4302540.7042336/94‑1 (MQ=60)

                                                                                           GGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAG  >  SRR4302540.3468616/1‑94 (MQ=60)

                                                                                           GGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAG  >  SRR4302540.9104004/1‑94 (MQ=60)

                                                                                           GGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAG  >  SRR4302540.4876088/1‑94 (MQ=60)

TTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAG  >  NC_000913/2259826‑2260010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`