Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,259,919	C→T	R156H (CGC→CAC)	fruK ←	fructose‑1‑phosphate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,259,919	0	C	T	99.5%	746.0 / NA	209	R156H (CGC→CAC)	fruK	fructose‑1‑phosphate kinase
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (108/100); minor base A (0/1); total (108/101)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 212 total aligned reads displayed.

GTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTC  >  NC_000913/2259830‑2260008

    cACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCAt                                                                                    <  1:913455/93‑1 (MQ=255)

     aCCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATc                                                                                   <  1:1124100/93‑1 (MQ=255)

       cAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCa                                                                                 <  1:1063211/93‑1 (MQ=255)

       cAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCa                                                                                 <  2:3876583/93‑1 (MQ=255)

        aGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAg                                                                                >  1:3879974/1‑93 (MQ=255)

         gCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGt                                                                               <  1:3087617/93‑1 (MQ=255)

          ccACGGTTCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAACGCAAGGACACTGACTACGCAGGAGAGTCACCCAGTc                                                                              <  2:5671655/93‑1 (MQ=255)

          ccACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTc                                                                              <  1:5362957/93‑1 (MQ=255)

          ccACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTc                                                                              <  1:3788007/93‑1 (MQ=255)

           cACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCg                                                                             >  1:726219/1‑93 (MQ=255)

           cACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCg                                                                             >  2:4125183/1‑93 (MQ=255)

            ccGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCgg                                                                            <  1:2263794/92‑1 (MQ=255)

            aCGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCgg                                                                            <  2:1110353/93‑1 (MQ=255)

            aCGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCgg                                                                            <  2:2413912/93‑1 (MQ=255)

            aCGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCgg                                                                            <  1:522246/93‑1 (MQ=255)

            aCGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCgg                                                                            >  2:5442811/1‑93 (MQ=255)

            aCGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCgg                                                                            >  2:1072456/1‑93 (MQ=255)

            aCGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCgg                                                                            >  2:5260190/1‑93 (MQ=255)

            aCGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCgg                                                                            <  2:4616587/93‑1 (MQ=255)

             cGGTGCCGCTTTCAAACCTGCTTCTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGt                                                                           >  2:4153827/1‑93 (MQ=255)

              ggTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTg                                                                          <  1:1789833/93‑1 (MQ=255)

                tGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGaa                                                                        <  1:1183126/93‑1 (MQ=255)

                 gCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAAc                                                                       <  1:1812134/93‑1 (MQ=255)

                 gCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAAc                                                                       <  2:3115659/93‑1 (MQ=255)

                   cGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGc                                                                     <  2:5830456/93‑1 (MQ=255)

                   cGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGc                                                                     >  2:1757972/1‑93 (MQ=255)

                    gCTTTCAACCCTGCTACTAACGCTTCACGGCTACTATCAAGGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCt                                                                    <  2:3714754/93‑1 (MQ=255)

                     cTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCtt                                                                   >  2:5795963/1‑93 (MQ=255)

                        tCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCg                                                                <  1:200246/93‑1 (MQ=255)

                        tCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCg                                                                <  1:5991314/93‑1 (MQ=255)

                         cAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCgg                                                               >  2:5060566/1‑93 (MQ=255)

                         cAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCgg                                                               <  1:2825467/93‑1 (MQ=255)

                            aCCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCTGTGAACGCTTCCGGGCt                                                            <  2:254000/93‑1 (MQ=255)

                             ccTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTg                                                           >  2:5360394/1‑93 (MQ=255)

                             ccTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTg                                                           >  2:601030/1‑93 (MQ=255)

                              cTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGa                                                          <  2:4823400/93‑1 (MQ=255)

                              cTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGa                                                          <  1:2659916/93‑1 (MQ=255)

                              cTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGa                                                          <  2:3442188/93‑1 (MQ=255)

                               tGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGAc                                                         <  1:2581680/93‑1 (MQ=255)

                                gCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACg                                                        <  1:4158949/93‑1 (MQ=255)

                                gCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACg                                                        >  1:5464522/1‑93 (MQ=255)

                                 ctactaACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGc                                                       <  2:3057235/93‑1 (MQ=255)

                                 ctactaACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGc                                                       >  1:5149589/1‑93 (MQ=255)

                                 ctactaACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGc                                                       >  1:5200406/1‑93 (MQ=255)

                                  tactaACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAAGGCTTCCGGGCTGACGcc                                                      >  1:991298/1‑93 (MQ=255)

                                  tactaACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGcc                                                      >  1:1514123/1‑93 (MQ=255)

                                   actaACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCt                                                     >  1:2370369/1‑93 (MQ=255)

                                   actaACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCt                                                     >  1:5391452/1‑93 (MQ=255)

                                     taACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGa                                                   >  2:3992598/1‑93 (MQ=255)

                                     taACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGa                                                   >  1:2145962/1‑93 (MQ=255)

                                      aaCGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGAc                                                  <  1:4909717/93‑1 (MQ=255)

                                      aaCGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGAc                                                  >  2:302739/1‑93 (MQ=255)

                                      aaCGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGAc                                                  >  1:2632685/1‑93 (MQ=255)

                                          cTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTa                                              <  1:6148463/93‑1 (MQ=255)

                                          cTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTa                                              <  2:5035952/93‑1 (MQ=255)

                                           ttCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTaa                                             >  1:6189143/1‑93 (MQ=255)

                                           ttCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTaa                                             >  2:5199121/1‑93 (MQ=255)

                                           ttCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTaa                                             <  1:195922/93‑1 (MQ=255)

                                           ttCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTaa                                             >  1:512141/1‑93 (MQ=255)

                                           ttCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGGGAACGCTTCCGGGCTGACGCCTGCCGGTaa                                             >  1:1267937/1‑93 (MQ=255)

                                            tCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAg                                            >  2:4435633/1‑93 (MQ=255)

                                            tCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAg                                            <  2:2367835/93‑1 (MQ=255)

                                             cACGTCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGc                                           <  2:4898214/93‑1 (MQ=255)

                                             cACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGc                                           <  1:6257807/93‑1 (MQ=255)

                                               cGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGATGACGCCTGACGGTAAGCtc                                         >  1:3397566/1‑92 (MQ=255)

                                                 gCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTcc                                       >  2:821057/1‑93 (MQ=255)

                                                  ctactaTCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCg                                      >  1:2513424/1‑93 (MQ=255)

                                                  ctactaTCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCg                                      <  1:2156273/93‑1 (MQ=255)

                                                   tactaTCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGc                                     <  2:631006/93‑1 (MQ=255)

                                                    actaTCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCt                                    >  1:4753758/1‑93 (MQ=255)

                                                       aTCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGac                                 >  2:4300348/1‑93 (MQ=255)

                                                         cAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGacac                               >  2:4182319/1‑93 (MQ=255)

                                                          aaaGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGAcaca                              >  1:3018687/1‑93 (MQ=255)

                                                          aaaGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGAcaca                              >  1:440304/1‑93 (MQ=255)

                                                          aaaGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGAcaca                              >  2:3958668/1‑93 (MQ=255)

                                                          aaaGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGAcaca                              <  1:5047605/93‑1 (MQ=255)

                                                            aGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGa                            <  1:3262012/93‑1 (MQ=255)

                                                             gATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGAc                           <  2:2140665/93‑1 (MQ=255)

                                                               tAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCa                         >  2:2764777/1‑93 (MQ=255)

                                                                aaTGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGGTTCCGCTGACCCAGACCat                        <  2:6166525/93‑1 (MQ=255)

                                                                aaTGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCat                        >  2:2213398/1‑93 (MQ=255)

                                                                 aTGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCata                       <  1:3111338/93‑1 (MQ=255)

                                                                  tGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCatat                      >  2:320623/1‑93 (MQ=255)

                                                                   gCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATc                     <  2:1900162/93‑1 (MQ=255)

                                                                     aaGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGa                   >  1:807775/1‑93 (MQ=255)

                                                                      aGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGaa                  >  1:4624787/1‑93 (MQ=255)

                                                                         acacTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTg               <  1:5888467/93‑1 (MQ=255)

                                                                          cacTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGa              >  1:3822417/1‑93 (MQ=255)

                                                                            cTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGAcc            <  1:5742372/93‑1 (MQ=255)

                                                                             tGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCg           >  1:5424965/1‑93 (MQ=255)

                                                                              gACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGa          >  1:399657/1‑93 (MQ=255)

                                                                              gACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGa          <  2:1320178/93‑1 (MQ=255)

                                                                               aCTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAg         >  2:728156/1‑93 (MQ=255)

                                                                                  aCGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCa      >  2:3327942/1‑93 (MQ=255)

                                                                                  aCGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCa      <  2:230235/93‑1 (MQ=255)

                                                                                   cGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAg     >  1:3477329/1‑93 (MQ=255)

                                                                                    gCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGc    >  1:2912581/1‑93 (MQ=255)

                                                                                     cAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCt   >  1:4464438/1‑93 (MQ=255)

                                                                                      aGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTc  >  1:3634791/1‑93 (MQ=255)

                                                                                      aGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTc  >  1:3971131/1‑93 (MQ=255)

GTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTC  >  NC_000913/2259830‑2260008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGAG  >  NC_000913/2259826‑2260012

TTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGT                                                                                               <  SRR4302533.706191/94‑1 (MQ=60)

 TAGGTTCCCCCAGCCATGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTG                                                                                              <  SRR4302533.514188/94‑1 (MQ=60)

   GGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAG                                                                                            >  SRR4302533.1183941/1‑94 (MQ=60)

        CACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATC                                                                                       <  SRR4302533.918823/94‑1 (MQ=60)

         ACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCC                                                                                      <  SRR4302533.1130770/94‑1 (MQ=60)

          CCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCA                                                                                     >  SRR4302533.5241007/1‑94 (MQ=60)

           CAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAG                                                                                    <  SRR4302533.1069459/94‑1 (MQ=60)

           CAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAG                                                                                    >  SRR4302533.3903648/1‑94 (MQ=60)

           CAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAG                                                                                    <  SRR4302533.6195301/94‑1 (MQ=60)

             GCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTC                                                                                  <  SRR4302533.3106600/94‑1 (MQ=60)

              CCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCG                                                                                 <  SRR4302533.3042489/94‑1 (MQ=60)

              CCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCG                                                                                 <  SRR4302533.3811204/94‑1 (MQ=60)

              CCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCG                                                                                 <  SRR4302533.5395166/94‑1 (MQ=60)

              CCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCG                                                                                 >  SRR4302533.730509/1‑94 (MQ=60)

                CCGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGT                                                                               <  SRR4302533.2277801/94‑1 (MQ=60)

                ACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGT                                                                               <  SRR4302533.525346/94‑1 (MQ=60)

                 CGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTG                                                                              <  SRR4302533.1701679/94‑1 (MQ=60)

                  GGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGA                                                                             <  SRR4302533.1800715/94‑1 (MQ=60)

                    TGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAAC                                                                           <  SRR4302533.1190232/94‑1 (MQ=60)

                    TGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAAC                                                                           >  SRR4302533.3272692/1‑94 (MQ=60)

                     GCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACG                                                                          <  SRR4302533.1823204/94‑1 (MQ=60)

                       CGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCT                                                                        <  SRR4302533.518024/94‑1 (MQ=60)

                       CGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCT                                                                        >  SRR4302533.5362622/1‑94 (MQ=60)

                         CTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTC                                                                      <  SRR4302533.241210/94‑1 (MQ=60)

                          TTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCC                                                                     <  SRR4302533.620573/94‑1 (MQ=60)

                           TTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCG                                                                    >  SRR4302533.1373178/1‑94 (MQ=60)

                            TCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGG                                                                   <  SRR4302533.6027603/94‑1 (MQ=60)

                            TCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGG                                                                   <  SRR4302533.201516/94‑1 (MQ=60)

                            TCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGG                                                                   >  SRR4302533.4674124/1‑94 (MQ=60)

                             CAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGG                                                                  <  SRR4302533.2842854/94‑1 (MQ=60)

                              AAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGC                                                                 >  SRR4302533.6288710/1‑94 (MQ=60)

                              AAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACGACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGC                                                                 <  SRR4302533.5193869/94‑1 (MQ=60)

                               AACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCT                                                                >  SRR4302533.5477779/1‑94 (MQ=60)

                                ACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTG                                                               <  SRR4302533.4456002/94‑1 (MQ=60)

                                  CTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGAC                                                             <  SRR4302533.5692433/94‑1 (MQ=60)

                                  CTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGAC                                                             >  SRR4302533.79867/1‑94 (MQ=60)

                                  CTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGAC                                                             <  SRR4302533.2676251/94‑1 (MQ=60)

                                   TGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACG                                                            >  SRR4302533.5497451/1‑94 (MQ=60)

                                   TGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACG                                                            >  SRR4302533.2094325/1‑94 (MQ=60)

                                   TGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACG                                                            <  SRR4302533.2597561/94‑1 (MQ=60)

                                    GCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGC                                                           <  SRR4302533.4184153/94‑1 (MQ=60)

                                    GCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGC                                                           >  SRR4302533.5180701/1‑94 (MQ=60)

                                    GCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGC                                                           >  SRR4302533.5231755/1‑94 (MQ=60)

                                     CTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAAGGCTTCCGGGCTGACGCC                                                          >  SRR4302533.997094/1‑94 (MQ=60)

                                     CTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCC                                                          >  SRR4302533.1523246/1‑94 (MQ=60)

                                      TACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCT                                                         >  SRR4302533.2385032/1‑94 (MQ=60)

                                      TACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCT                                                         >  SRR4302533.5423846/1‑94 (MQ=60)

                                       ACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTG                                                        <  SRR4302533.3847129/94‑1 (MQ=60)

                                        CTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGA                                                       >  SRR4302533.2159179/1‑94 (MQ=60)

                                         TAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGAC                                                      >  SRR4302533.2648865/1‑94 (MQ=60)

                                         TAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGAC                                                      <  SRR4302533.1817138/94‑1 (MQ=60)

                                          AACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACG                                                     <  SRR4302533.4939460/94‑1 (MQ=60)

                                           ACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGG                                                    <  SRR4302533.2783009/94‑1 (MQ=60)

                                           ACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGG                                                    >  SRR4302533.2666536/1‑94 (MQ=60)

                                            CGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGT                                                   >  SRR4302533.3360519/1‑94 (MQ=60)

                                            CGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGT                                                   <  SRR4302533.996501/94‑1 (MQ=60)

                                              CTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAA                                                 >  SRR4302533.6226285/1‑94 (MQ=60)

                                              CTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAA                                                 <  SRR4302533.5166443/94‑1 (MQ=60)

                                              CTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAA                                                 <  SRR4302533.6185477/94‑1 (MQ=60)

                                              CTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAA                                                 <  SRR4302533.2159719/94‑1 (MQ=60)

                                              CTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAA                                                 >  SRR4302533.515184/1‑94 (MQ=60)

                                              CTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGGGAACGCTTCCGGGCTGACGCCTGCCGGTAA                                                 >  SRR4302533.1275527/1‑94 (MQ=60)

                                               TTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAG                                                <  SRR4302533.197160/94‑1 (MQ=60)

                                                 CACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCT                                              <  SRR4302533.6295229/94‑1 (MQ=60)

                                                  ACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGATGACGCCTGACGGTAAGCTC                                             >  SRR4302533.3418549/1‑94 (MQ=60)

                                                   CGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTC                                            >  SRR4302533.1254249/1‑94 (MQ=60)

                                                    GGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCC                                           >  SRR4302533.3471518/1‑94 (MQ=60)

                                                    GGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCC                                           <  SRR4302533.4000357/94‑1 (MQ=60)

                                                    GGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCC                                           <  SRR4302533.2000995/94‑1 (MQ=60)

                                                     GCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCG                                          >  SRR4302533.2528913/1‑94 (MQ=60)

                                                      CTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGC                                         <  SRR4302533.2169549/94‑1 (MQ=60)

                                                       TACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCT                                        <  SRR4302533.463657/94‑1 (MQ=60)

                                                       TACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCT                                        <  SRR4302533.2704035/94‑1 (MQ=60)

                                                       TACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCT                                        >  SRR4302533.4782575/1‑94 (MQ=60)

                                                         CTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGA                                      >  SRR4302533.5137729/1‑94 (MQ=60)

                                                         CTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGA                                      >  SRR4302533.357914/1‑94 (MQ=60)

                                                             CAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAAGGCTTCCGGGCTGACGCCTGCCGGTAAGCTTCCGCTGACACA                                  >  SRR4302533.1859259/1‑94 (MQ=60)

                                                             CAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACA                                  >  SRR4302533.442933/1‑94 (MQ=60)

                                                             CAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACA                                  >  SRR4302533.4408560/1‑94 (MQ=60)

                                                             CAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACA                                  >  SRR4302533.5418220/1‑94 (MQ=60)

                                                             CAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACA                                  >  SRR4302533.3037168/1‑94 (MQ=60)

                                                              AAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAG                                 >  SRR4302533.291256/1‑94 (MQ=60)

                                                              AAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAG                                 <  SRR4302533.5078136/94‑1 (MQ=60)

                                                               AAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGA                                <  SRR4302533.5832093/94‑1 (MQ=60)

                                                               AAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGA                                <  SRR4302533.4621967/94‑1 (MQ=60)

                                                                AGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGAC                               <  SRR4302533.3282136/94‑1 (MQ=60)

                                                                 GATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACC                              <  SRR4302533.5011986/94‑1 (MQ=60)

                                                                  ATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCA                             >  SRR4302533.4162675/1‑94 (MQ=60)

                                                                   TAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCAT                            <  SRR4302533.3522617/94‑1 (MQ=60)

                                                                     ATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATAT                          <  SRR4302533.3130464/94‑1 (MQ=60)

                                                                     ATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATAT                          >  SRR4302533.293432/1‑94 (MQ=60)

                                                                     ATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATAT                          <  SRR4302533.692748/94‑1 (MQ=60)

                                                                      TGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATC                         <  SRR4302533.3816568/94‑1 (MQ=60)

                                                                       GCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCG                        >  SRR4302533.3952161/1‑94 (MQ=60)

                                                                        CAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGA                       <  SRR4302533.5270433/94‑1 (MQ=60)

                                                                        CAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGA                       >  SRR4302533.812523/1‑94 (MQ=60)

                                                                         AAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAA                      >  SRR4302533.4652819/1‑94 (MQ=60)

                                                                           GGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACT                    <  SRR4302533.5987286/94‑1 (MQ=60)

                                                                            GACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTG                   <  SRR4302533.2105265/94‑1 (MQ=60)

                                                                             ACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGA                  <  SRR4302533.2223101/94‑1 (MQ=60)

                                                                             ACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGA                  >  SRR4302533.3845787/1‑94 (MQ=60)

                                                                             ACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGA                  <  SRR4302533.5924043/94‑1 (MQ=60)

                                                                                CTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCG               >  SRR4302533.5457637/1‑94 (MQ=60)

                                                                                CTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCG               <  SRR4302533.5777022/94‑1 (MQ=60)

                                                                                 TGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGA              >  SRR4302533.916750/1‑94 (MQ=60)

                                                                                 TGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGA              >  SRR4302533.402069/1‑94 (MQ=60)

                                                                                  GACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAG             <  SRR4302533.1099096/94‑1 (MQ=60)

                                                                                   ACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGC            >  SRR4302533.2647772/1‑94 (MQ=60)

                                                                                    CTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGCGCC           >  SRR4302533.544036/1‑94 (MQ=60)

                                                                                    CTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCC           >  SRR4302533.479016/1‑94 (MQ=60)

                                                                                    CTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCC           >  SRR4302533.3754256/1‑94 (MQ=60)

                                                                                      ACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAG         <  SRR4302533.6110486/94‑1 (MQ=60)

                                                                                      ACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAG         >  SRR4302533.3498785/1‑94 (MQ=60)

                                                                                      ACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAG         <  SRR4302533.2538826/94‑1 (MQ=60)

                                                                                       CGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGC        >  SRR4302533.2930456/1‑94 (MQ=60)

                                                                                        GCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCT       >  SRR4302533.4491441/1‑94 (MQ=60)

                                                                                         CAGGTGAGTTATCCAGTCAGTGATCGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTC      <  SRR4302533.3129865/94‑1 (MQ=60)

                                                                                         CAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTC      >  SRR4302533.3995317/1‑94 (MQ=60)

                                                                                         CAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTC      >  SRR4302533.3657145/1‑94 (MQ=60)

                                                                                         CAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTC      <  SRR4302533.2335986/94‑1 (MQ=60)

                                                                                           GGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAG    >  SRR4302533.387808/1‑94 (MQ=60)

                                                                                           GGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAG    <  SRR4302533.4458345/94‑1 (MQ=60)

                                                                                           GGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAG    <  SRR4302533.2377500/94‑1 (MQ=60)

                                                                                            GTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGA   <  SRR4302533.1484654/94‑1 (MQ=60)

                                                                                            GTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGA   <  SRR4302533.3233967/94‑1 (MQ=60)

                                                                                             TGAGTCACCCAGTCGGTGAACGCTTCCGGGCGGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGAG  <  SRR4302533.701461/94‑1 (MQ=60)

TTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGAG  >  NC_000913/2259826‑2260012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`