Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,261,454 G→A A21V (GCG→GTG)  fruB ← PTS system, fructose‑specific IIA/fpr component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,261,4540GA98.9% 968.8 / NA 286A21V (GCG→GTG) fruBPTS system, fructose‑specific IIA/fpr component
Reads supporting (aligned to +/- strand):  ref base G (0/2);  major base A (164/116);  minor base C (0/3);  total (164/122)
Fisher's exact test for biased strand distribution p-value = 7.33e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 295 total aligned reads displayed.

GAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGA  >  NC_000913/2261364‑2261544
                                                                                          |                                                                                          
gAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCAcc                                                                                          <  2:6845237/93‑1 (MQ=255)
  ggTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACctc                                                                                        >  2:1911195/1‑93 (MQ=255)
   gTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACctct                                                                                       <  1:7662778/93‑1 (MQ=255)
   gTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACctct                                                                                       >  1:2394296/1‑93 (MQ=255)
    tttGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCtctt                                                                                      >  1:6716503/1‑93 (MQ=255)
    tttGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCtctt                                                                                      >  1:4749661/1‑93 (MQ=255)
       gCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTcttctt                                                                                   >  2:5438886/1‑93 (MQ=255)
        cTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCttt                                                                                  <  2:754536/93‑1 (MQ=255)
         tGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTg                                                                                 >  1:1592071/1‑93 (MQ=255)
          gTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGt                                                                                >  1:8146705/1‑93 (MQ=255)
          gTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGt                                                                                <  2:2819435/93‑1 (MQ=255)
           ttCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGtc                                                                               <  1:1114747/93‑1 (MQ=255)
           ttCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGtc                                                                               <  2:9968227/93‑1 (MQ=255)
              gcgcgcCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTcc                                                                            <  2:1543765/93‑1 (MQ=255)
                 cgcCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGc                                                                         >  1:2965164/1‑93 (MQ=255)
                    cAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCtt                                                                      <  2:2367144/93‑1 (MQ=255)
                       cATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTc                                                                   >  1:1687636/1‑93 (MQ=255)
                       cATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTc                                                                   <  1:2113702/93‑1 (MQ=255)
                         tGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGc                                                                 >  1:959021/1‑93 (MQ=255)
                         tGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGc                                                                 >  1:741566/1‑93 (MQ=255)
                         tGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGc                                                                 >  1:2562736/1‑93 (MQ=255)
                           ccATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGccc                                                               >  1:1306510/1‑93 (MQ=255)
                             aTTGACGTAGCCTTCTGCTACATTCCCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCgg                                                             >  2:2691678/1‑93 (MQ=255)
                             aTTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCgg                                                             >  1:3985805/1‑93 (MQ=255)
                              ttGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCgga                                                            <  1:1210465/93‑1 (MQ=255)
                              ttGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCgga                                                            >  2:3183433/1‑93 (MQ=255)
                              ttGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCgga                                                            >  2:4843496/1‑93 (MQ=255)
                              ttGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCgga                                                            <  2:7772710/93‑1 (MQ=255)
                              ttGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCaga                                                            >  2:3478275/1‑93 (MQ=255)
                              ttGACCTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCgga                                                            <  1:3278104/93‑1 (MQ=255)
                                gACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCggatg                                                          >  1:9244618/1‑93 (MQ=255)
                                 aCGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCggatgg                                                         <  2:2251822/93‑1 (MQ=255)
                                 aCGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCggatgg                                                         >  2:5159453/1‑93 (MQ=255)
                                  cGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCggatgga                                                        >  1:2689640/1‑93 (MQ=255)
                                   gTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCggatggat                                                       <  1:7843573/93‑1 (MQ=255)
                                   gTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCggatggat                                                       >  1:3335673/1‑93 (MQ=255)
                                    tAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGgatggatg                                                      >  2:2541163/1‑93 (MQ=255)
                                      gCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTc                                                    >  2:8226290/1‑93 (MQ=255)
                                      gCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTc                                                    >  1:6877834/1‑93 (MQ=255)
                                      gCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTc                                                    >  2:4867637/1‑93 (MQ=255)
                                         ttCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTg                                                 >  1:4833122/1‑93 (MQ=255)
                                         ttCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTg                                                 <  1:3508351/93‑1 (MQ=255)
                                          tCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGt                                                >  1:7100688/1‑93 (MQ=255)
                                          tCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGt                                                >  1:5934747/1‑93 (MQ=255)
                                                aCATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGAt                                          >  1:4072532/1‑93 (MQ=255)
                                                 cATTCCCGGCCTGCCCCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATa                                         <  1:1407091/93‑1 (MQ=255)
                                                 cATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATa                                         >  2:982564/1‑93 (MQ=255)
                                                  aTTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATaa                                        >  1:5566564/1‑93 (MQ=255)
                                                  aTTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATaa                                        >  1:4339359/1‑93 (MQ=255)
                                                  aTTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATaa                                        <  2:6583388/93‑1 (MQ=255)
                                                    tACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACt                                      >  2:5854328/1‑93 (MQ=255)
                                                      ccGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTgg                                    >  1:9501455/1‑93 (MQ=255)
                                                       cGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGa                                   <  1:3880667/93‑1 (MQ=255)
                                                       cGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGa                                   <  1:6540930/93‑1 (MQ=255)
                                                       cGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGa                                   >  1:8072798/1‑93 (MQ=255)
                                                        ggCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGaa                                  <  2:1120279/93‑1 (MQ=255)
                                                           cTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACAt                               >  1:3599376/1‑93 (MQ=255)
                                                            tGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATa                              >  2:9458581/1‑93 (MQ=255)
                                                            tGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATa                              >  1:4518329/1‑93 (MQ=255)
                                                            tGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATa                              <  2:7182750/93‑1 (MQ=255)
                                                              cACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGt                            <  2:3769588/93‑1 (MQ=255)
                                                               aCCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGtt                           <  1:3762244/93‑1 (MQ=255)
                                                                ccAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATTACTGGAACATAGTtc                          >  2:7867928/1‑93 (MQ=255)
                                                                 cAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTtct                         <  2:8034059/93‑1 (MQ=255)
                                                                 cAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTtct                         >  1:4884005/1‑93 (MQ=255)
                                                                  aGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTtctc                        <  2:6160895/93‑1 (MQ=255)
                                                                   gcgcCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTctcc                       >  2:9593693/1‑93 (MQ=255)
                                                                   gcgcCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTctcc                       >  2:2105430/1‑93 (MQ=255)
                                                                   gcgcAGCAGCGACCTGTCAAATCACCTCTTCTTTGTCTCCGTCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTctcc                       <  2:7450171/93‑1 (MQ=255)
                                                                    cgccgcAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTctcct                      <  1:9850568/93‑1 (MQ=255)
                                                                     gccgcAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTctcctc                     <  1:2290554/93‑1 (MQ=255)
                                                                       cgcAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTAGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCctctc                   <  2:2700664/93‑1 (MQ=255)
                                                                        gcAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCctctct                  >  2:4158837/1‑93 (MQ=255)
                                                                         cAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCtt                 >  1:7291213/1‑93 (MQ=255)
                                                                          aGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTg                >  2:4667948/1‑93 (MQ=255)
                                                                           gCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGc               >  2:9385427/1‑93 (MQ=255)
                                                                             gACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTg             <  1:8863003/93‑1 (MQ=255)
                                                                              gccTGGCGAATCCCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGa            <  1:2019283/92‑1 (MQ=255)
                                                                              aCCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGa            >  2:4637719/1‑93 (MQ=255)
                                                                              aCCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGa            >  2:6250918/1‑93 (MQ=255)
                                                                               ccTGGCGAATCCCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGaa           <  2:4001020/93‑1 (MQ=255)
                                                                                cTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAAt          >  2:8110303/1‑93 (MQ=255)
                                                                                cTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAAt          >  1:1195142/1‑93 (MQ=255)
                                                                                cTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAAt          >  2:2921735/1‑93 (MQ=255)
                                                                                cTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAAt          <  1:9428886/93‑1 (MQ=255)
                                                                                 tGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAAtt         >  2:6348954/1‑93 (MQ=255)
                                                                                 tGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAAtt         >  2:6716707/1‑93 (MQ=255)
                                                                                  ggCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTg        <  1:2480168/93‑1 (MQ=255)
                                                                                   gCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGa       >  2:6071363/1‑93 (MQ=255)
                                                                                   gCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGa       <  2:8415497/93‑1 (MQ=255)
                                                                                    cGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTACTCTCTTGCTGAATTGaa      >  1:9119417/1‑93 (MQ=255)
                                                                                     gAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGaaa     <  1:5116092/93‑1 (MQ=255)
                                                                                     gAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGaaa     <  1:1911195/93‑1 (MQ=255)
                                                                                     gAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGaaa     <  2:6948560/93‑1 (MQ=255)
                                                                                      aaTCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAAc    <  2:6548402/93‑1 (MQ=255)
                                                                                      aaTCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAAc    >  1:7444442/1‑93 (MQ=255)
                                                                                       aTCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACg   >  1:4144981/1‑93 (MQ=255)
                                                                                       aTCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACg   >  2:9009806/1‑93 (MQ=255)
                                                                                       aTCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACg   >  1:7654319/1‑93 (MQ=255)
                                                                                       aTCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACg   >  1:8589552/1‑93 (MQ=255)
                                                                                          |                                                                                          
GAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGA  >  NC_000913/2261364‑2261544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTC  >  NC_000913/2261361‑2261547
                                                                                             |                                                                                             
GTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCA                                                                                               >  SRR4302540.4907709/1‑94 (MQ=60)
GTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCA                                                                                               >  SRR4302540.4907710/1‑94 (MQ=60)
  TGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACC                                                                                             >  SRR4302540.2930373/1‑94 (MQ=60)
   GAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCT                                                                                            <  SRR4302540.9955023/94‑1 (MQ=60)
   GAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCT                                                                                            <  SRR4302540.1664016/94‑1 (MQ=60)
     GGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCT                                                                                          <  SRR4302540.7049714/94‑1 (MQ=60)
     GGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCT                                                                                          >  SRR4302540.4085392/1‑94 (MQ=60)
     GGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCT                                                                                          >  SRR4302540.2420095/1‑94 (MQ=60)
      GTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTT                                                                                         >  SRR4302540.4800350/1‑94 (MQ=60)
      GTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTT                                                                                         <  SRR4302540.7749084/94‑1 (MQ=60)
      GTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTT                                                                                         >  SRR4302540.6790600/1‑94 (MQ=60)
      GTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTT                                                                                         <  SRR4302540.1333756/94‑1 (MQ=60)
          GCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTT                                                                                     <  SRR4302540.2435065/94‑1 (MQ=60)
           CTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTG                                                                                    >  SRR4302540.1609464/1‑94 (MQ=60)
            TGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGT                                                                                   >  SRR4302540.6653703/1‑94 (MQ=60)
            TGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGT                                                                                   >  SRR4302540.8241278/1‑94 (MQ=60)
              TTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCT                                                                                 <  SRR4302540.1126293/94‑1 (MQ=60)
                CGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCC                                                                               >  SRR4302540.30859/1‑94 (MQ=60)
                 GCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCG                                                                              <  SRR4302540.3137021/94‑1 (MQ=60)
                  CGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGG                                                                             >  SRR4302540.8484237/1‑94 (MQ=60)
                   GCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGCATCACCTCTTCTTTGTCTCCGGC                                                                            >  SRR4302540.3970879/1‑94 (MQ=60)
                   GCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGC                                                                            >  SRR4302540.2997884/1‑94 (MQ=60)
                    CGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCC                                                                           >  SRR4302540.3478908/1‑94 (MQ=60)
                      CCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTT                                                                         >  SRR4302540.1197711/1‑94 (MQ=60)
                       CAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTT                                                                        >  SRR4302540.4434330/1‑94 (MQ=60)
                       CAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGACTGCACCAGCGCCGCAGCGACCCGGCGAATCACCTCTTCTTTGTCTCCGGCCTTT                                                                        >  SRR4302540.77383/1‑94 (MQ=60)
                         GCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTC                                                                      >  SRR4302540.1705940/1‑94 (MQ=60)
                         GCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTC                                                                      >  SRR4302540.1705939/1‑94 (MQ=60)
                         GCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTC                                                                      <  SRR4302540.2656348/94‑1 (MQ=60)
                         GCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTC                                                                      >  SRR4302540.2088056/1‑94 (MQ=60)
                          CATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCG                                                                     >  SRR4302540.1544642/1‑94 (MQ=60)
                          CATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCG                                                                     <  SRR4302540.2136387/94‑1 (MQ=60)
                          CATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCG                                                                     >  SRR4302540.8576168/1‑94 (MQ=60)
                           ATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGC                                                                    >  SRR4302540.3729472/1‑94 (MQ=60)
                           ATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGC                                                                    >  SRR4302540.968952/1‑94 (MQ=60)
                           ATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGC                                                                    >  SRR4302540.749483/1‑94 (MQ=60)
                           ATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGC                                                                    >  SRR4302540.2590364/1‑94 (MQ=60)
                           ATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGC                                                                    >  SRR4302540.5625473/1‑94 (MQ=60)
                            TGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCC                                                                   >  SRR4302540.7801231/1‑94 (MQ=60)
                             GCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCC                                                                  >  SRR4302540.1320063/1‑94 (MQ=60)
                             GCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCC                                                                  >  SRR4302540.4193285/1‑94 (MQ=60)
                             GCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCC                                                                  >  SRR4302540.3735783/1‑94 (MQ=60)
                              CCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCG                                                                 <  SRR4302540.8902105/94‑1 (MQ=60)
                               CATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGG                                                                >  SRR4302540.4028724/1‑94 (MQ=60)
                                 TTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGAT                                                              <  SRR4302540.1222875/94‑1 (MQ=60)
                                 TTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGAT                                                              <  SRR4302540.8916876/94‑1 (MQ=60)
                                 TTGACCTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGAT                                                              <  SRR4302540.3313905/94‑1 (MQ=60)
                                 TCGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCCCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGAT                                                              <  SRR4302540.433288/94‑1 (MQ=60)
                                  TGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATG                                                             >  SRR4302540.9355321/1‑94 (MQ=60)
                                    ACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGA                                                           >  SRR4302540.2718699/1‑94 (MQ=60)
                                     CGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGAT                                                          >  SRR4302540.5779478/1‑94 (MQ=60)
                                     CGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGAT                                                          >  SRR4302540.3372308/1‑94 (MQ=60)
                                      GTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATG                                                         <  SRR4302540.7935222/94‑1 (MQ=60)
                                       TAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGT                                                        >  SRR4302540.2387965/1‑94 (MQ=60)
                                        AGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTC                                                       >  SRR4302540.6954069/1‑94 (MQ=60)
                                          CCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCT                                                     <  SRR4302540.2836274/94‑1 (MQ=60)
                                          CCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCT                                                     >  SRR4302540.1216970/1‑94 (MQ=60)
                                          CCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCT                                                     <  SRR4302540.750314/94‑1 (MQ=60)
                                          CCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCT                                                     >  SRR4302540.8179724/1‑94 (MQ=60)
                                           CTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTG                                                    >  SRR4302540.4884897/1‑94 (MQ=60)
                                           CTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTG                                                    <  SRR4302540.4146945/94‑1 (MQ=60)
                                           CTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTG                                                    >  SRR4302540.3627214/1‑94 (MQ=60)
                                           CTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTG                                                    >  SRR4302540.4884899/1‑94 (MQ=60)
                                           CTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTG                                                    >  SRR4302540.5776742/1‑94 (MQ=60)
                                            TTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCTGGATGGATGTCCTGT                                                   <  SRR4302540.7600799/94‑1 (MQ=60)
                                            TTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGT                                                   <  SRR4302540.3546565/94‑1 (MQ=60)
                                            TTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGT                                                   >  SRR4302540.6000343/1‑94 (MQ=60)
                                            TTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGT                                                   >  SRR4302540.7179698/1‑94 (MQ=60)
                                            TTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGT                                                   <  SRR4302540.10047018/94‑1 (MQ=60)
                                            TTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGT                                                   >  SRR4302540.4641212/1‑94 (MQ=60)
                                            TTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGT                                                   <  SRR4302540.7600800/94‑1 (MQ=60)
                                             TCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTA                                                  >  SRR4302540.7161652/1‑94 (MQ=60)
                                              CTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTAC                                                 >  SRR4302540.9676076/1‑94 (MQ=60)
                                              CTGCTACATTACCAGACTGCACCAGCGCAGCAGCGACCTGGAGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTAC                                                 <  SRR4302540.9082220/94‑1 (MQ=60)
                                               TGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACG                                                >  SRR4302540.2293011/1‑94 (MQ=60)
                                                 CTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGA                                              >  SRR4302540.7839887/1‑94 (MQ=60)
                                                  TACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGAT                                             >  SRR4302540.9327089/1‑94 (MQ=60)
                                                  TACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGAT                                             >  SRR4302540.4116430/1‑94 (MQ=60)
                                                  TACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGAT                                             <  SRR4302540.175317/94‑1 (MQ=60)
                                                   ACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATA                                            <  SRR4302540.3818322/94‑1 (MQ=60)
                                                    CATTCCCGGCCTGCCCCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAA                                           <  SRR4302540.1422243/94‑1 (MQ=60)
                                                    CATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAA                                           >  SRR4302540.4386599/1‑94 (MQ=60)
                                                    CATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAA                                           >  SRR4302540.5627861/1‑94 (MQ=60)
                                                      TTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACT                                         <  SRR4302540.4504416/94‑1 (MQ=60)
                                                       TACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTG                                        >  SRR4302540.4877636/1‑94 (MQ=60)
                                                        ACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGG                                       >  SRR4302540.9614041/1‑94 (MQ=60)
                                                         CCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGA                                      >  SRR4302540.8166468/1‑94 (MQ=60)
                                                         CCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGA                                      <  SRR4302540.3564374/94‑1 (MQ=60)
                                                          CGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAA                                     <  SRR4302540.6613512/94‑1 (MQ=60)
                                                          CGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAA                                     >  SRR4302540.2171410/1‑94 (MQ=60)
                                                          CGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAA                                     <  SRR4302540.3922833/94‑1 (MQ=60)
                                                            GCCTGCCCCAGCGCCGCAGCGTCCTGGCCAATCCCCTCTTCTTTGTCACCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACA                                   <  SRR4302540.2554110/94‑1 (MQ=60)
                                                            GCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACA                                   >  SRR4302540.1945261/1‑94 (MQ=60)
                                                            GCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACA                                   <  SRR4302540.7185444/94‑1 (MQ=60)
                                                            GCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACA                                   <  SRR4302540.2624200/94‑1 (MQ=60)
                                                             CCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACAT                                  >  SRR4302540.8181248/1‑94 (MQ=60)
                                                             CCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACAT                                  >  SRR4302540.3638709/1‑94 (MQ=60)
                                                             CCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACAT                                  <  SRR4302540.750644/94‑1 (MQ=60)
                                                              CTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATG                                 >  SRR4302540.6888612/1‑94 (MQ=60)
                                                              CTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATA                                 >  SRR4302540.6888614/1‑94 (MQ=60)
                                                              CTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATA                                 >  SRR4302540.6375045/1‑94 (MQ=60)
                                                              CTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATA                                 >  SRR4302540.8133125/1‑94 (MQ=60)
                                                              CTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATA                                 >  SRR4302540.4567269/1‑94 (MQ=60)
                                                              CTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATA                                 >  SRR4302540.4029697/1‑94 (MQ=60)
                                                               GGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAG                                <  SRR4302540.7388548/94‑1 (MQ=60)
                                                                 CACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTT                              <  SRR4302540.256113/94‑1 (MQ=60)
                                                                 CACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTT                              <  SRR4302540.958039/94‑1 (MQ=60)
                                                                 CACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTT                              <  SRR4302540.3938745/94‑1 (MQ=60)
                                                                  ACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTC                             <  SRR4302540.3803288/94‑1 (MQ=60)
                                                                  ACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTC                             <  SRR4302540.7462506/94‑1 (MQ=60)
                                                                  ACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTC                             >  SRR4302540.3529100/1‑94 (MQ=60)
                                                                  ACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTC                             >  SRR4302540.9221116/1‑94 (MQ=60)
                                                                  ACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTC                             >  SRR4302540.121895/1‑94 (MQ=60)
                                                                   CCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCT                            >  SRR4302540.4936464/1‑94 (MQ=60)
                                                                    CAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTC                           <  SRR4302540.9330524/94‑1 (MQ=60)
                                                                     AGCGCCGCAGCGACCTGGCGAATCACCTCGTCTTTGTCTCCGGCCTTCTAGCCCAGATGGATGTCgcacaagaacaactacaacagcggtaccc                          >  SRR4302540.4087033/1‑65 (MQ=47)
                                                                       CGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTC                        <  SRR4302540.9965703/94‑1 (MQ=60)
                                                                       CGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTC                        >  SRR4302540.2364292/1‑94 (MQ=60)
                                                                        GCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCT                       >  SRR4302540.2063324/1‑94 (MQ=60)
                                                                        GCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCT                       <  SRR4302540.2315202/94‑1 (MQ=60)
                                                                        GCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCT                       <  SRR4302540.1574562/94‑1 (MQ=60)
                                                                         CCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTC                      >  SRR4302540.3359922/1‑94 (MQ=60)
                                                                         CCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTC                      <  SRR4302540.4913674/94‑1 (MQ=60)
                                                                         CCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTC                      >  SRR4302540.1641854/1‑94 (MQ=60)
                                                                         CCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTC                      >  SRR4302540.3359921/1‑94 (MQ=60)
                                                                         CCGCAGCCACCTTGCGAATCACCTCTTCCTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTC                      <  SRR4302540.4913663/94‑1 (MQ=60)
                                                                           GCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTT                    >  SRR4302540.4181109/1‑94 (MQ=60)
                                                                           GCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTT                    >  SRR4302540.9717550/1‑94 (MQ=60)
                                                                           GCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTT                    >  SRR4302540.7372576/1‑94 (MQ=60)
                                                                           GCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTT                    <  SRR4302540.9576339/94‑1 (MQ=60)
                                                                              GCGACGTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCT                 <  SRR4302540.1361334/94‑1 (MQ=60)
                                                                              GCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCT                 >  SRR4302540.1935511/1‑94 (MQ=60)
                                                                                GACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGA               <  SRR4302540.8965565/94‑1 (MQ=60)
                                                                                 GCCTGGCGAATCCCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAA              <  SRR4302540.2041109/94‑1 (MQ=60)
                                                                                  CCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAAT             >  SRR4302540.1297384/1‑94 (MQ=60)
                                                                                  CCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAAT             >  SRR4302540.1207450/1‑94 (MQ=60)
                                                                                   CTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACGTAGTTCTCCTCTCTTGCTGAATT            >  SRR4302540.9451866/1‑94 (MQ=60)
                                                                                   CTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATT            <  SRR4302540.4657283/94‑1 (MQ=60)
                                                                                   CTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATT            <  SRR4302540.9540905/94‑1 (MQ=60)
                                                                                   CTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATT            >  SRR4302540.1241843/1‑94 (MQ=60)
                                                                                   CTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATT            >  SRR4302540.736177/1‑94 (MQ=60)
                                                                                   CTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATT            >  SRR4302540.250341/1‑94 (MQ=60)
                                                                                     GGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGA          <  SRR4302540.2506883/94‑1 (MQ=60)
                                                                                     GGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGA          <  SRR4302540.6265848/94‑1 (MQ=60)
                                                                                      GCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATTGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAA         <  SRR4302540.4990590/94‑1 (MQ=60)
                                                                                      GCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTACTCTCTTGCTGAATTGAA         >  SRR4302540.9229029/1‑94 (MQ=60)
                                                                                       CGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAA        >  SRR4302540.8116492/1‑94 (MQ=60)
                                                                                        GAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGACAC       >  SRR4302540.502057/1‑94 (MQ=60)
                                                                                        GAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAAC       >  SRR4302540.7527386/1‑94 (MQ=60)
                                                                                        GAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAAC       <  SRR4302540.5171479/94‑1 (MQ=60)
                                                                                        GAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAAC       <  SRR4302540.5171480/94‑1 (MQ=60)
                                                                                        GAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAAC       <  SRR4302540.1932076/94‑1 (MQ=60)
                                                                                        GAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAAC       >  SRR4302540.9669162/1‑94 (MQ=60)
                                                                                         AATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACG      <  SRR4302540.9376583/94‑1 (MQ=60)
                                                                                         AATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACG      >  SRR4302540.8689408/1‑94 (MQ=60)
                                                                                         AATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACG      >  SRR4302540.7740490/1‑94 (MQ=60)
                                                                                         AATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACG      >  SRR4302540.4189839/1‑94 (MQ=60)
                                                                                          ATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGA     >  SRR4302540.1608822/1‑94 (MQ=60)
                                                                                          ATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGA     >  SRR4302540.1601305/1‑94 (MQ=60)
                                                                                          ATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGA     >  SRR4302540.4928219/1‑94 (MQ=60)
                                                                                          ATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGA     >  SRR4302540.4928220/1‑94 (MQ=60)
                                                                                          ATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGA     >  SRR4302540.5793137/1‑94 (MQ=60)
                                                                                           TCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATTTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGAT    <  SRR4302540.6655336/94‑1 (MQ=60)
                                                                                           TCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGAT    <  SRR4302540.1963927/94‑1 (MQ=60)
                                                                                           TCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGAT    <  SRR4302540.1963926/94‑1 (MQ=60)
                                                                                           TCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGAT    <  SRR4302540.6655335/94‑1 (MQ=60)
                                                                                            CACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATATTTCTCCTCTCTTGCTGAATTGAAACGATT   >  SRR4302540.10161685/1‑94 (MQ=60)
                                                                                            CACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATT   >  SRR4302540.5639115/1‑94 (MQ=60)
                                                                                            CACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATT   <  SRR4302540.6393153/94‑1 (MQ=60)
                                                                                            CACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATT   >  SRR4302540.7752244/1‑94 (MQ=60)
                                                                                            CACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATT   <  SRR4302540.9899010/94‑1 (MQ=60)
                                                                                             ACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTC  >  SRR4302540.4688915/1‑94 (MQ=60)
                                                                                             ACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTC  >  SRR4302540.7367001/1‑94 (MQ=60)
                                                                                             ACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTC  <  SRR4302540.1363208/94‑1 (MQ=60)
                                                                                             ACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTC  <  SRR4302540.1363201/94‑1 (MQ=60)
                                                                                             ACCTCTTCTTTGCCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTC  >  SRR4302540.10096300/1‑94 (MQ=60)
                                                                                             |                                                                                             
GTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTC  >  NC_000913/2261361‑2261547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑