Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,261,454 G→A A21V (GCG→GTG)  fruB ← PTS system, fructose‑specific IIA/fpr component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,261,4540GA99.5% 669.8 / NA 196A21V (GCG→GTG) fruBPTS system, fructose‑specific IIA/fpr component
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (98/96);  total (98/98)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 200 total aligned reads displayed.

GAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATT  >  NC_000913/2261364‑2261546
                                                                                          |                                                                                            
gAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCAcc                                                                                            >  1:2496515/1‑93 (MQ=255)
  ggTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCTGCAGCGACCTGGCGAATCACctc                                                                                          >  1:4881355/1‑93 (MQ=255)
   gTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACctct                                                                                         >  1:3393985/1‑93 (MQ=255)
   gTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACctct                                                                                         <  1:6208671/93‑1 (MQ=255)
    tttGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCtctt                                                                                        >  1:685599/1‑93 (MQ=255)
    tttGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCtctt                                                                                        <  1:3993079/93‑1 (MQ=255)
    tttGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATGACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCtctt                                                                                        <  1:4090168/93‑1 (MQ=255)
     ttGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCtcttc                                                                                       >  2:5169682/1‑93 (MQ=255)
     ttGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCtcttc                                                                                       <  1:5178521/93‑1 (MQ=255)
     ttGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCtcttc                                                                                       <  1:3774345/93‑1 (MQ=255)
      tGCTGTTCGCGCGCCAGCATGCCATTGACGTAGTCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCtcttct                                                                                      <  1:2439015/93‑1 (MQ=255)
      tGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCtcttct                                                                                      >  2:344444/1‑93 (MQ=255)
        cTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCttt                                                                                    >  1:1608471/1‑93 (MQ=255)
        cTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCCGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCttt                                                                                    <  2:5523012/93‑1 (MQ=255)
         tGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTg                                                                                   >  1:3294855/1‑93 (MQ=255)
         tGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTg                                                                                   >  1:2389075/1‑93 (MQ=255)
          gTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGt                                                                                  <  1:3936743/93‑1 (MQ=255)
          gTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGt                                                                                  >  1:5650768/1‑93 (MQ=255)
           ttCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGtc                                                                                 <  2:555735/93‑1 (MQ=255)
            tCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGtct                                                                                >  1:1178474/1‑93 (MQ=255)
             cgcgcgCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGtctc                                                                               <  1:3782575/93‑1 (MQ=255)
              gcgcgcCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTcc                                                                              <  2:1159830/93‑1 (MQ=255)
               cgcgcCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCg                                                                             <  1:5324202/93‑1 (MQ=255)
               cgcgcCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCg                                                                             <  1:1386676/93‑1 (MQ=255)
                  gcCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGcc                                                                          <  1:4982150/93‑1 (MQ=255)
                  gcCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGcc                                                                          >  2:329711/1‑93 (MQ=255)
                   ccAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCt                                                                         >  2:2106965/1‑93 (MQ=255)
                    cAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCtt                                                                        <  1:1082064/93‑1 (MQ=255)
                    cAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCtt                                                                        >  1:4162108/1‑93 (MQ=255)
                     aGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCttt                                                                       >  2:46251/1‑93 (MQ=255)
                     aGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCttt                                                                       >  2:2374613/1‑93 (MQ=255)
                      gCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCtttt                                                                      >  2:65889/1‑93 (MQ=255)
                      gCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCtttt                                                                      <  1:6081067/93‑1 (MQ=255)
                       cATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTc                                                                     >  1:5533495/1‑93 (MQ=255)
                        aTGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCg                                                                    >  1:1828045/1‑93 (MQ=255)
                          tccATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGcc                                                                  <  1:516557/92‑1 (MQ=255)
                          gCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGcc                                                                  <  2:3622847/93‑1 (MQ=255)
                          gCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGcc                                                                  >  2:3699237/1‑93 (MQ=255)
                           ccATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGccc                                                                 <  1:1939838/93‑1 (MQ=255)
                            cATTGACGTAGCCTTCTGCTTCATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCg                                                                >  2:6064637/1‑93 (MQ=255)
                            cATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCg                                                                >  1:2860622/1‑93 (MQ=255)
                              ttGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCgga                                                              >  2:4408416/1‑93 (MQ=255)
                              ttGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCgga                                                              <  1:203168/93‑1 (MQ=255)
                                 aCGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCggatgg                                                           <  2:4496381/93‑1 (MQ=255)
                                   gTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCggatggat                                                         <  2:4447995/93‑1 (MQ=255)
                                      gCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTc                                                      >  1:2665213/1‑93 (MQ=255)
                                       ccTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTcc                                                     >  2:1660954/1‑93 (MQ=255)
                                        cTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCt                                                    <  1:3784231/93‑1 (MQ=255)
                                        cTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCt                                                    <  2:4381799/93‑1 (MQ=255)
                                          tCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGt                                                  <  2:2932942/93‑1 (MQ=255)
                                           cTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTa                                                 <  1:2279732/93‑1 (MQ=255)
                                            tGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTAc                                                <  2:4149478/93‑1 (MQ=255)
                                            tGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTAc                                                <  1:6244748/93‑1 (MQ=255)
                                               tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCTTTTTCGCCCGGATGGATGTCCTGTACGGa                                             <  2:1885300/93‑1 (MQ=255)
                                               tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGa                                             <  2:1412292/93‑1 (MQ=255)
                                               tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGa                                             >  1:3499150/1‑93 (MQ=255)
                                               tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGa                                             >  2:1018217/1‑93 (MQ=255)
                                                aCATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTCCGGAt                                            <  1:627851/93‑1 (MQ=255)
                                                aCATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGAt                                            <  2:1479729/93‑1 (MQ=255)
                                                aCATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGAt                                            >  1:2542712/1‑93 (MQ=255)
                                                 cATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGGCCGGATGGATGTCCTGTACGGATa                                           >  2:4482016/1‑93 (MQ=255)
                                                   ttACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAAc                                         >  2:6170860/1‑93 (MQ=255)
                                                   ttACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAAc                                         >  2:2291886/1‑93 (MQ=255)
                                                     aCCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTg                                       >  2:2684601/1‑93 (MQ=255)
                                                      ccGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTgg                                      >  1:705947/1‑93 (MQ=255)
                                                      ccGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTgg                                      >  1:1233639/1‑93 (MQ=255)
                                                      ccGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTgg                                      <  1:2079901/93‑1 (MQ=255)
                                                        ggCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGaa                                    <  2:2550173/93‑1 (MQ=255)
                                                           cTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACAt                                 >  2:3869729/1‑93 (MQ=255)
                                                            tGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATa                                <  2:1755829/93‑1 (MQ=255)
                                                              cACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGt                              >  1:4287482/1‑93 (MQ=255)
                                                              cACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGt                              <  2:6269827/93‑1 (MQ=255)
                                                                ccAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTtc                            >  2:3548879/1‑93 (MQ=255)
                                                                   gcgcCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTctcc                         >  1:4192847/1‑93 (MQ=255)
                                                                     cccgcAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTctcctc                       <  2:4021423/92‑1 (MQ=255)
                                                                         cAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATCGATGTCCTGTACGGAGAACTGGAACAGAGTTCTCCTCTCtt                   >  1:3131247/1‑93 (MQ=255)
                                                                          aGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTg                  >  1:5880542/1‑93 (MQ=255)
                                                                          aGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTg                  >  2:4227952/1‑93 (MQ=255)
                                                                          aGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTg                  <  2:1599681/93‑1 (MQ=255)
                                                                           gCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGc                 >  1:1904915/1‑93 (MQ=255)
                                                                           gCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGc                 <  2:6133471/93‑1 (MQ=255)
                                                                            cGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCt                >  2:422657/1‑93 (MQ=255)
                                                                            cGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCt                <  2:3036536/93‑1 (MQ=255)
                                                                            cGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCt                <  2:1815640/93‑1 (MQ=255)
                                                                              aCCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGa              <  1:1676778/93‑1 (MQ=255)
                                                                              aCCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGa              >  2:492081/1‑93 (MQ=255)
                                                                               ccTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGaa             <  1:1447118/93‑1 (MQ=255)
                                                                                 tGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAAtt           >  1:3063696/1‑93 (MQ=255)
                                                                                 tGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAAtt           <  1:2697973/93‑1 (MQ=255)
                                                                                 tGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAAtt           <  2:3843604/93‑1 (MQ=255)
                                                                                 tGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAAtt           >  1:444835/1‑93 (MQ=255)
                                                                                 cggCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAAtt           <  1:469970/92‑1 (MQ=255)
                                                                                  ggCGAATCCCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTg          <  2:2139008/93‑1 (MQ=255)
                                                                                     gAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGaaa       <  2:4619231/93‑1 (MQ=255)
                                                                                     gAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGaaa       <  2:4949707/93‑1 (MQ=255)
                                                                                     gAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGaaa       >  1:1355385/1‑93 (MQ=255)
                                                                                     gAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGaaa       >  2:3904624/1‑93 (MQ=255)
                                                                                     gAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAAATGGAACATAGTTCTCCTCTCTTGCTGAATTGaaa       >  2:2177355/1‑93 (MQ=255)
                                                                                      aaTCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAAc      <  2:5868581/93‑1 (MQ=255)
                                                                                          gCCTCATCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCGCCGCTCTTGCTGAATTGAAACGAtt  <  1:2091912/93‑1 (MQ=255)
                                                                                          |                                                                                            
GAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATT  >  NC_000913/2261364‑2261546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTC  >  NC_000913/2261362‑2261547
                                                                                            |                                                                                             
TTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCAC                                                                                              >  SRR4302533.4267503/1‑94 (MQ=60)
 TGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACC                                                                                             >  SRR4302533.2511915/1‑94 (MQ=60)
  GAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCT                                                                                            <  SRR4302533.2651641/94‑1 (MQ=60)
   AGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCTGCAGCGACCTGGCGAATCACCTC                                                                                           >  SRR4302533.4910928/1‑94 (MQ=60)
    GGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCT                                                                                          >  SRR4302533.3414946/1‑94 (MQ=60)
     GTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTT                                                                                         >  SRR4302533.689654/1‑94 (MQ=60)
     GTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTT                                                                                         <  SRR4302533.6245911/94‑1 (MQ=60)
      TTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTC                                                                                        <  SRR4302533.4017391/94‑1 (MQ=60)
      TTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATGACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTC                                                                                        <  SRR4302533.4115068/94‑1 (MQ=60)
       TTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCT                                                                                       <  SRR4302533.3835102/94‑1 (MQ=60)
       TTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCT                                                                                       <  SRR4302533.5209794/94‑1 (MQ=60)
       TTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCT                                                                                       <  SRR4302533.5213929/94‑1 (MQ=60)
       TTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCT                                                                                       <  SRR4302533.1741737/94‑1 (MQ=60)
       TTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCT                                                                                       <  SRR4302533.4129394/94‑1 (MQ=60)
       TTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCT                                                                                       <  SRR4302533.3797483/94‑1 (MQ=60)
        TGCTGTTCGCGCGCCAGCATGCCATTGACGTAGTCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTT                                                                                      <  SRR4302533.2454077/94‑1 (MQ=60)
        TGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTT                                                                                      >  SRR4302533.3023442/1‑94 (MQ=60)
         GCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTT                                                                                     >  SRR4302533.1618229/1‑94 (MQ=60)
          CTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTG                                                                                    >  SRR4302533.2689089/1‑94 (MQ=60)
          CTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTG                                                                                    >  SRR4302533.3315189/1‑94 (MQ=60)
          CTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTG                                                                                    >  SRR4302533.2403831/1‑94 (MQ=60)
           TGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGT                                                                                   >  SRR4302533.5684926/1‑94 (MQ=60)
            GTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTTCTACATTACCGGCCTGCCCCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTC                                                                                  <  SRR4302533.3960774/94‑1 (MQ=60)
            GTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTC                                                                                  <  SRR4302533.3960775/94‑1 (MQ=60)
             TTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCT                                                                                 <  SRR4302533.678090/94‑1 (MQ=60)
             TTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCT                                                                                 >  SRR4302533.1185551/1‑94 (MQ=60)
               CGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCC                                                                               <  SRR4302533.5045899/94‑1 (MQ=60)
               CGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCC                                                                               <  SRR4302533.3805741/94‑1 (MQ=60)
                 CGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGG                                                                             <  SRR4302533.1395022/94‑1 (MQ=60)
                 CGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGG                                                                             <  SRR4302533.4684303/94‑1 (MQ=60)
                 CGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGG                                                                             <  SRR4302533.5356110/94‑1 (MQ=60)
                  GCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGC                                                                            >  SRR4302533.3368932/1‑94 (MQ=60)
                    GCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCT                                                                          >  SRR4302533.1102528/1‑94 (MQ=60)
                    GCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCT                                                                          >  SRR4302533.1971522/1‑94 (MQ=60)
                    GCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCT                                                                          <  SRR4302533.5012356/94‑1 (MQ=60)
                     CCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTT                                                                         >  SRR4302533.2491357/1‑94 (MQ=60)
                     CCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTT                                                                         >  SRR4302533.4187330/1‑94 (MQ=60)
                      CAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTT                                                                        <  SRR4302533.1088448/94‑1 (MQ=60)
                       AGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTT                                                                       <  SRR4302533.227551/94‑1 (MQ=60)
                        GCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTC                                                                      <  SRR4302533.2515241/94‑1 (MQ=60)
                        GCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTC                                                                      >  SRR4302533.5566903/1‑94 (MQ=60)
                        GCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTC                                                                      <  SRR4302533.2050977/94‑1 (MQ=60)
                        GCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTC                                                                      <  SRR4302533.6117840/94‑1 (MQ=60)
                         CATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCG                                                                     >  SRR4302533.4333239/1‑94 (MQ=60)
                         CATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCG                                                                     >  SRR4302533.1839207/1‑94 (MQ=60)
                         CATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCG                                                                     <  SRR4302533.3966576/94‑1 (MQ=60)
                            TCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCC                                                                  <  SRR4302533.519623/94‑1 (MQ=60)
                             CCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCG                                                                 >  SRR4302533.1582623/1‑94 (MQ=60)
                             CCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCG                                                                 <  SRR4302533.1951670/94‑1 (MQ=60)
                             CCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCG                                                                 >  SRR4302533.2878221/1‑94 (MQ=60)
                             CCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCG                                                                 >  SRR4302533.2098472/1‑94 (MQ=60)
                               ATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGA                                                               >  SRR4302533.5454744/1‑94 (MQ=60)
                                TTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGAT                                                              <  SRR4302533.204460/94‑1 (MQ=60)
                                TGGACGTAGCCTTCTGCTACATTACCGGCCTGCCCCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGAT                                                              <  SRR4302533.3814648/94‑1 (MQ=60)
                                 TGGCGTAGCCTTCTTCTAAATTACCGGCCTGCCCCAGCCCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATG                                                             <  SRR4302533.3997798/94‑1 (MQ=60)
                                 TGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATG                                                             <  SRR4302533.3122493/94‑1 (MQ=60)
                                   ACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGA                                                           <  SRR4302533.6191691/94‑1 (MQ=60)
                                      TAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGT                                                        >  SRR4302533.4776371/1‑94 (MQ=60)
                                       AGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTC                                                       >  SRR4302533.2681569/1‑94 (MQ=60)
                                          CTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTG                                                    <  SRR4302533.4269864/94‑1 (MQ=60)
                                          CTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTG                                                    <  SRR4302533.3807404/94‑1 (MQ=60)
                                            TCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTA                                                  <  SRR4302533.1636785/94‑1 (MQ=60)
                                             CTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTGCTGTAC                                                 >  SRR4302533.877742/1‑94 (MQ=60)
                                             CTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTAC                                                 <  SRR4302533.2293849/94‑1 (MQ=60)
                                              TGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACG                                                <  SRR4302533.6282127/94‑1 (MQ=60)
                                              TGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACG                                                <  SRR4302533.4200870/94‑1 (MQ=60)
                                                CTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGA                                              >  SRR4302533.3520729/1‑94 (MQ=60)
                                                 TACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGAT                                             >  SRR4302533.704508/1‑94 (MQ=60)
                                                 TACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGAT                                             >  SRR4302533.2558360/1‑94 (MQ=60)
                                                  ACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTCCGGATA                                            <  SRR4302533.631541/94‑1 (MQ=60)
                                                   CATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAA                                           >  SRR4302533.3540583/1‑94 (MQ=60)
                                                   CATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAA                                           <  SRR4302533.5599177/94‑1 (MQ=60)
                                                   CATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAA                                           >  SRR4302533.1861360/1‑94 (MQ=60)
                                                       ACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGG                                       >  SRR4302533.710115/1‑94 (MQ=60)
                                                       ACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGG                                       >  SRR4302533.1241019/1‑94 (MQ=60)
                                                        CCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGA                                      <  SRR4302533.1215162/94‑1 (MQ=60)
                                                        CCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGA                                      <  SRR4302533.2092698/94‑1 (MQ=60)
                                                          GGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAAC                                    >  SRR4302533.2443943/1‑94 (MQ=60)
                                                             CTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATA                                 >  SRR4302533.1255404/1‑94 (MQ=60)
                                                              TGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAG                                >  SRR4302533.1411089/1‑94 (MQ=60)
                                                               GCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGT                               >  SRR4302533.4313263/1‑94 (MQ=60)
                                                               GCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGT                               >  SRR4302533.629743/1‑94 (MQ=60)
                                                                 ACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTC                             <  SRR4302533.3212929/94‑1 (MQ=60)
                                                                   CAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTC                           <  SRR4302533.3802617/94‑1 (MQ=60)
                                                                   CAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTC                           <  SRR4302533.1386143/94‑1 (MQ=60)
                                                                   CAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCATTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTC                           >  SRR4302533.4112678/1‑94 (MQ=60)
                                                                    AGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCC                          >  SRR4302533.4218225/1‑94 (MQ=60)
                                                                    AGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCC                          <  SRR4302533.6254559/94‑1 (MQ=60)
                                                                         CGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCT                     >  SRR4302533.2365875/1‑94 (MQ=60)
                                                                          GCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTT                    <  SRR4302533.5197507/94‑1 (MQ=60)
                                                                          GCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTT                    >  SRR4302533.5879459/1‑94 (MQ=60)
                                                                          GCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATCGATGTCCTGTACGGAGAACTGGAACAGAGTTCTCCTCTCTT                    >  SRR4302533.3150500/1‑94 (MQ=60)
                                                                           CAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTG                   >  SRR4302533.5916078/1‑94 (MQ=60)
                                                                            AGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGC                  >  SRR4302533.1916537/1‑94 (MQ=60)
                                                                                ACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAA              <  SRR4302533.1686931/94‑1 (MQ=60)
                                                                                 CCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAAT             <  SRR4302533.1455850/94‑1 (MQ=60)
                                                                                  CTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATT            >  SRR4302533.3082507/1‑94 (MQ=60)
                                                                                  CTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATT            >  SRR4302533.447496/1‑94 (MQ=60)
                                                                                   TGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTG           <  SRR4302533.4915549/94‑1 (MQ=60)
                                                                                   TGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTG           <  SRR4302533.2714506/94‑1 (MQ=60)
                                                                                   CGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTG           <  SRR4302533.472778/94‑1 (MQ=60)
                                                                                    GGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGA          <  SRR4302533.2723024/94‑1 (MQ=60)
                                                                                      CGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAA        >  SRR4302533.1363531/1‑94 (MQ=60)
                                                                                      CGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAA        >  SRR4302533.3459408/1‑94 (MQ=60)
                                                                                       GAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAAC       >  SRR4302533.1932568/1‑94 (MQ=60)
                                                                                        AATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACG      <  SRR4302533.5956312/94‑1 (MQ=60)
                                                                                         ATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGA     >  SRR4302533.3265445/1‑94 (MQ=60)
                                                                                         ATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGA     >  SRR4302533.2874857/1‑94 (MQ=60)
                                                                                           CACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATT   >  SRR4302533.4944403/1‑94 (MQ=60)
                                                                                           CACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATT   >  SRR4302533.2343014/1‑94 (MQ=60)
                                                                                            GCCTCATCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCGCCGCTCTTGCTGAATTGAAACGATTC  <  SRR4302533.2104795/94‑1 (MQ=60)
                                                                                            ACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTC  >  SRR4302533.4076186/1‑94 (MQ=60)
                                                                                            |                                                                                             
TTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTC  >  NC_000913/2261362‑2261547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑